Calibration auxiliary structure for fluorescent immune layer detection reagent

Abstract

The utility model discloses an auxiliary structure for fluorescent immune layer detection reagent calibration, which relates to the technical field of reagent calibration and comprises a guide hopper, a mixing cylinder is arranged in the middle of the bottom end of the guide hopper, a sealing tube is detachably mounted at the bottom of the mixing cylinder, a thin film is arranged at the bottom of the sealing tube, and the thin film is arranged in the guide hopper. Stand columns are arranged at the four corners of the bottom end of the guide hopper, the bottom ends of the four stand columns are jointly connected with an upper sealing plate, a crevasse converter is placed at the top end of the upper sealing plate, the top of the crevasse converter is matched with the bottom of a sealing pipe, a liquid outlet is formed in one side of the crevasse converter, and a liquid outlet is formed in the other side of the crevasse converter. A crevasse cone is arranged in the middle of the interior of the crevasse converter. According to the utility model, a series of structures are arranged, so that a reagent can be dropped on the test paper without a dropper or other tools during operation, the situation that liquid cannot accurately fall on the preset position of the test paper due to hand shaking or other factors of an operator is avoided, and the calibration efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of reagent calibration technology, specifically to an auxiliary structure for calibrating a fluorescent immunoassay reagent. Background Technology

[0002] Fluorescent immunochromatographic assay reagent calibration is an important process to ensure the accuracy and reliability of the assay reagents. It is mainly used to verify the performance indicators of fluorescent immunochromatographic assay reagents, including both quantitative and qualitative methods. Fluorescent immunochromatography uses fluorescently labeled specific antibodies or antigens as standard reagents for the analysis, identification and quantitative determination of the corresponding antigens or antibodies.

[0003] Most current calibration methods for fluorescent immunoassay reagents involve the operator mixing the reagent and standard sample, and then using a dropper or other aspiration device to apply the liquid to a predetermined position on the test strip. This method is prone to errors due to operator tremors or external factors, resulting in the liquid not falling precisely at the predetermined position, thus affecting the accuracy of the calibration results and is also inefficient. Utility Model Content

[0004] The purpose of this invention is to provide an auxiliary structure for calibrating a fluorescent immunoassay reagent to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a calibration auxiliary structure for a fluorescent immunoassay reagent, comprising a guide bucket, a mixing cylinder disposed at the middle of the bottom of the guide bucket, a sealing tube detachably installed at the bottom of the mixing cylinder, a thin film disposed at the bottom of the sealing tube, columns disposed at the four corners of the bottom of the guide bucket, an upper sealing plate connected to the bottom of the four columns, a break converter disposed at the top of the upper sealing plate, the top of the break converter matching the bottom of the sealing tube, an outlet disposed on one side of the break converter, a break cone disposed at the middle of the interior of the break converter, a drainer matching the outlet disposed at the top of the upper sealing plate near the break converter, a locking hole matching the break converter being opened at the top of the upper sealing plate near the drainer, a through opening being opened at the bottom of the drainer and the top of the upper sealing plate, a base hinged to the bottom of the upper sealing plate, a placement groove being opened at the top of the base, and a test strip being placed inside the placement groove.

[0006] Preferably, the test strip has a waterproof membrane at its top, and the top of the waterproof membrane has a contact opening that matches the opening. The waterproof membrane protects the top of the test strip, preventing liquid from seeping into the bottom of the upper sealing plate when it comes into contact with the test strip, thus avoiding stickiness and making it difficult to clean.

[0007] Preferably, the top of the upper sealing plate below the guide bucket is provided with four positioning holes that match the columns, so that the four columns can be placed at the top of the upper sealing plate in a predetermined position, ensuring that the sealing tube can be connected with the breaking converter.

[0008] Preferably, an auxiliary groove is provided on one side of the rupture cone near the liquid outlet. When the rupture cone punctures the film at the bottom of the sealing tube, the film may adhere to the surface of the rupture cone. At this time, the liquid can flow down through the auxiliary groove to avoid blockage.

[0009] Preferably, one end of the upper sealing plate is provided with a notch, and two buckles matching the notch are provided at the top edge of the base. The buckles and the notch work together to lock the upper sealing plate to the top of the base.

[0010] Preferably, an agitator is provided on the inner wall of the mixing cylinder through a slot, and a vertical shaft is provided at the middle position of the agitator. The top of the vertical shaft extends to the top of the guide bucket. After the sample to be tested is poured into the mixing cylinder, the vertical shaft is rotated to make the agitator rotate inside the mixing cylinder to fully mix the internal liquid.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] This fluorescent immunochromatographic assay calibration auxiliary structure features a guide funnel at the top of the mixing cylinder for easy sample and reagent addition. The sample and reagent flow into the mixing cylinder for mixing. After mixing, the mixing cylinder is placed on top of the upper sealing plate, aligning the sealing tube at the bottom of the mixing cylinder with the puncture converter at the top of the upper sealing plate. The puncture cone inside the puncture converter punctures the membrane at the bottom of the sealing tube, allowing the liquid to flow through the puncture converter and guide to the test strip position. This simplifies the calibration process, eliminating the need for droppers or other tools to apply reagents to the test strip. It also prevents operator errors due to shaky hands or other factors from causing the liquid to fall inaccurately at the intended location on the test strip, thus improving calibration efficiency. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a schematic diagram of the overall structure of this utility model in disassembled state;

[0015] Figure 3 For the present utility model Figure 2 Enlarged schematic diagram of the structure at point A in the middle;

[0016] Figure 4 This is a schematic diagram of the disassembled base structure of this utility model;

[0017] Figure 5This is a cross-sectional schematic diagram of the structure of the break converter of this utility model;

[0018] Figure 6 This is a partial cross-sectional schematic diagram of the mixing cylinder structure of this utility model.

[0019] In the diagram: 1. Guide hopper; 2. Mixing cylinder; 3. Base; 4. Top sealing plate; 5. Column; 6. Drainage device; 7. Positioning hole; 8. Locking hole; 9. Break converter; 10. Notch; 11. Contact port; 12. Waterproof membrane; 13. Test paper; 14. Buckle; 15. Through port; 16. Placement slot; 17. Break cone; 18. Auxiliary slot; 19. Liquid outlet; 20. Sealing tube; 21. Membrane; 22. Vertical shaft; 23. Agitator. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0022] like Figures 1 to 6As shown in the figure, this embodiment provides a calibration auxiliary structure for a fluorescent immunoassay reagent, including a guide bucket 1. A mixing cylinder 2 is located at the center of the bottom of the guide bucket 1. The inner wall of the guide bucket 1 is a smooth arc-shaped surface. Calibration samples and standards are poured into the guide bucket 1, allowing the liquid to flow into the mixing cylinder 2 for mixing. A sealing tube 20 is detachably installed at the bottom of the mixing cylinder 2, and a film 21 is provided at the bottom of the sealing tube 20. A column 5 is provided at each of the four corners of the bottom of the guide bucket 1. When reagent samples are added, the guide bucket 1 can be stably placed on a flat surface by the four columns 5. The upper sealing plate 4 is connected to the upper sealing plate 4. A rupture converter 9 is placed on the top of the upper sealing plate 4. The top of the rupture converter 9 matches the bottom of the sealing tube 20. An outlet 19 is provided on one side of the rupture converter 9. A rupture cone 17 is provided in the middle of the interior of the rupture converter 9. After the reagent sample is mixed, the four columns 5 are placed on the top of the upper sealing plate 4, so that the bottom of the sealing tube 20 is aligned with the rupture converter 9 and inserted. The thin film 21 at the bottom of the sealing tube 20 is punctured by the rupture cone 17 inside the rupture converter 9, so that the sample inside the mixing tube 2 flows out to the outlet 19. A guide 6, matching the outlet 19, is located near the break converter 9. When the liquid inside the mixing cylinder 2 flows into the outlet 19, the liquid enters the guide 6 along the outlet 19. A locking hole 8, matching the break converter 9, is provided at the top of the upper sealing plate 4 near the guide 6. A through-hole 15 is provided at the top of the upper sealing plate 4 at the bottom of the guide 6. The guide 6 guides the mixed sample to the through-hole 15. A base 3 is hinged to the bottom of the upper sealing plate 4. A placement groove 16 is provided at the top of the base 3. The test strip 13 is placed inside the placement groove 16. The test strip 13 is positioned by the placement slot 16, allowing it to receive the sample left inside the opening 15. Inside the test strip 13, the sample pad, latex pad, NC membrane, and absorbent paper are sequentially overlapped. The latex pad is coated with lanthanide-labeled monoclonal antibody I for the test antigen and fluorescent dye-labeled BSA goat polyclonal antibody. The detection line of the NC membrane is coated with a mixture of monoclonal antibody II for the test antigen and BSA. The control line is coated with goat anti-mouse IgG antibody or rabbit anti-mouse IgG antibody. With this structure and composition, the detection precision can be controlled within 3%-6% using the BSA calibration system, meeting the clinical requirements for accurate quantitative detection and facilitating reagent calibration.

[0023] Specifically, a waterproof membrane 12 is provided at the top of the test strip 13. The top of the waterproof membrane 12 has a contact port 11 that matches the opening 15. The waterproof membrane 12 protects the top of the test strip 13, preventing liquid from seeping into the bottom of the upper sealing plate 4 when it comes into contact with the test strip 13, thus avoiding stickiness and making it difficult to clean.

[0024] Furthermore, the top of the upper sealing plate 4 below the guide bucket 1 is provided with four positioning holes 7 that match the columns 5, so that the four columns 5 can be placed at the top of the upper sealing plate 4 in a predetermined position, ensuring that the sealing tube 20 can be connected with the breaking converter 9.

[0025] Furthermore, an auxiliary groove 18 is provided on one side of the rupture cone 17 near the liquid outlet 19. When the rupture cone 17 punctures the film 21 at the bottom of the sealing tube 20, the film 21 may adhere to the surface of the rupture cone 17. At this time, the liquid can flow down through the auxiliary groove 18 to avoid blockage.

[0026] Furthermore, one end of the upper sealing plate 4 is provided with a notch 10, and two buckles 14 that match the notch 10 are provided at the top edge of the base 3. The buckles 14 and the notch 10 work together to lock the upper sealing plate 4 to the top of the base 3.

[0027] Furthermore, an agitator 23 is installed on the inner wall of the mixing cylinder 2 through a slot, and a vertical shaft 22 is installed at the middle position of the agitator 23. The top of the vertical shaft 22 extends out of the top of the guide bucket 1. After the sample to be tested is poured into the mixing cylinder 2, the vertical shaft 22 is rotated to make the agitator 23 rotate inside the mixing cylinder 2 to drive the internal liquid to mix thoroughly.

[0028] The usage method of this embodiment is as follows: When calibrating the fluorescent immunoassay reagent, the guide bucket 1 can be stably placed on a flat surface by the four pillars 5. Then, the standard sample and reagent are poured into the guide bucket 1, and the reagent and sample will flow into the mixing cylinder 2. Then, the vertical shaft 22 is rotated to make the stirrer 23 rotate inside the mixing cylinder 2 to fully mix the internal liquid. After mixing, the four pillars 5 are placed on the top of the upper sealing plate 4, so that the bottom of the sealing tube 20 is aligned with the rupture converter 9 and inserted. The thin film 21 set at the bottom of the sealing tube 20 is punctured by the rupture cone 17 inside the rupture converter 9, so that the sample inside the mixing cylinder 2 flows out to the outlet 19. When the liquid inside the mixing cylinder 2 flows to the outlet... When the liquid enters the outlet 19, the liquid will flow into the drain 6 through the outlet 19. The drain 6 will guide the mixed sample to the opening 15. The test strip 13 can receive the sample left inside the opening 15. Inside the test strip 13, the sample pad, latex pad, NC membrane and absorbent paper are sequentially overlapped. The latex pad is coated with monoclonal antibody I of the test antigen labeled with lanthanide element and BSA goat polyclonal antibody labeled with fluorescent dye. The detection line of the NC membrane is coated with a mixture of monoclonal antibody II of the test antigen and BSA. The quality control line is coated with goat anti-mouse IgG antibody or rabbit anti-mouse IgG antibody. With this structure and composition, the detection precision can be controlled at 3%-6% using the BSA calibration system, which meets the clinical requirements for accurate quantitative detection and facilitates reagent calibration.

[0029] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A calibration auxiliary structure for a fluorescent immunoassay reagent, comprising a guide chamber (1), characterized in that: A mixing cylinder (2) is provided at the middle of the bottom end of the guide bucket (1). A sealing tube (20) is detachably installed at the bottom of the mixing cylinder (2). A film (21) is provided at the bottom of the sealing tube (20). A column (5) is provided at each of the four corners of the bottom end of the guide bucket (1). The bottom ends of the four columns (5) are connected to an upper sealing plate (4). A break converter (9) is placed at the top of the upper sealing plate (4). The top of the break converter (9) matches the bottom of the sealing tube (20). An outlet (19) is provided on one side of the break converter (9). A rupture cone (17) is provided in the middle of the interior of the upper sealing plate (4). A drainer (6) matching the outlet (19) is provided at the top of the upper sealing plate (4) near the rupture converter (9). A locking hole (8) matching the rupture converter (9) is provided at the top of the upper sealing plate (4) near the drainer (6). A through opening (15) is provided at the top of the upper sealing plate (4) at the bottom of the drainer (6). A base (3) is hinged to the bottom of the upper sealing plate (4). A placement groove (16) is provided at the top of the base (3). A test strip (13) is placed inside the placement groove (16).

2. The calibration auxiliary structure for a fluorescent immunoassay reagent according to claim 1, characterized in that: The test paper (13) is provided with a waterproof membrane (12) at the top, and the top of the waterproof membrane (12) is provided with a contact port (11) that matches the opening (15).

3. The calibration auxiliary structure for a fluorescent immunoassay reagent according to claim 1, characterized in that: The top of the upper sealing plate (4) below the guide bucket (1) is provided with four positioning holes (7) that match the column (5).

4. The calibration auxiliary structure for a fluorescent immunoassay reagent according to claim 1, characterized in that: An auxiliary groove (18) is provided on one side of the rupture cone (17) near the liquid outlet (19).

5. The calibration auxiliary structure for a fluorescent immunoassay reagent according to claim 1, characterized in that: One end of the upper sealing plate (4) is provided with a notch (10), and two buckles (14) matching the notch (10) are provided at the top edge of the base (3).

6. The calibration auxiliary structure for a fluorescent immunoassay reagent according to claim 1, characterized in that: The inner wall of the mixing cylinder (2) is provided with an agitator (23) through a slot, and a vertical shaft (22) is provided at the middle position of the agitator (23), with the top of the vertical shaft (22) extending out of the top of the guide bucket (1).

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