Multi-row reaction device for polypeptide synthesis

Abstract

The utility model belongs to the field of polypeptide synthesis, and provides a multi-row reaction device for polypeptide synthesis, which comprises a reaction box, a storage component and a swing component, a box cover is hinged on the reaction box, a plurality of rotating shafts are rotatably connected in the reaction box, the storage component is arranged in the middle of the rotating shafts, and a reaction column is arranged in the storage component. A swinging assembly is arranged on the outer side of the reaction box and is used for driving the multiple groups of rotating shafts to rotate to drive the reaction column to swing; the reciprocating driving piece is matched with the T-shaped frame, the T-shaped frame can be driven to drive the row teeth to continuously and transversely reciprocate under the combined action of the motor, the rotating disc and the push column, the row teeth are meshed with the gears to drive the reaction columns to shake left and right, polypeptide synthesis reaction is promoted, reaction efficiency is improved, energy consumption and mechanical abrasion are reduced, and the service life of the reaction columns is prolonged. The push column is adjustably mounted on the turntable through the positioning piece, so that the shaking amplitude of the reaction column can be adjusted as required, and the operability is relatively high.

Description

Technical Field

[0001] This invention belongs to the field of polypeptide synthesis, specifically a multi-row reaction device for polypeptide synthesis. Background Technology

[0002] Polypeptides are bioactive substances that are related to various cellular functions in organisms. Their molecular structure is between that of amino acids and proteins. They are compounds formed by multiple amino acids linked together by peptide bonds in a certain sequence. A multi-stage reaction device for polypeptide synthesis is a device used for the batch synthesis of polypeptides by solid-phase synthesis. This device usually has multiple reaction channels and can carry out multiple polypeptide synthesis reactions simultaneously, thereby improving production efficiency.

[0003] Chinese patent CN219273023U discloses a multi-row reaction device for polypeptide synthesis. The device places the polypeptide into a reaction column and seals it. Then, the reaction column is passed through a first support plate to the bottom and enters a groove. An electric push rod is activated to drive the connecting block to move back and forth. The connecting block drives the gear to rotate through a rack. The gear drives the first support plate to rotate through a bearing, thereby causing the reaction column to shake. A fan is activated to blow air onto the heating rod.

[0004] However, the aforementioned peptide synthesis reaction apparatus uses an electric push rod as a power source to drive the reaction column to shake. During the shaking process, the electric push rod needs to be continuously extended and shortened, which leads to increased energy consumption and aggravated mechanical wear, and it is inconvenient to adjust the shaking amplitude.

[0005] To address the problems raised in the background art, those skilled in the art have proposed a multi-row reaction apparatus for polypeptide synthesis. Utility Model Content

[0006] To address the aforementioned technical problems, this invention provides a multi-row reaction device for polypeptide synthesis, which solves the problems in the prior art where an electric push rod is used as a power source to drive the reaction column to shake, resulting in increased energy consumption, aggravated mechanical wear, and inconvenience in adjusting the shaking amplitude.

[0007] A multi-row reaction apparatus for polypeptide synthesis, comprising:

[0008] The reaction chamber has a hinged lid and multiple rotating shafts inside. A storage assembly is located in the middle of the rotating shafts, and a reaction column is located inside the storage assembly.

[0009] A swing assembly, located outside the reaction chamber, drives multiple sets of rotating shafts to swing the reaction column. It is covered by a protective cover mounted on the outer wall of the reaction chamber, and a maintenance door is hinged to the protective cover.

[0010] The heating wire mesh is located inside the reaction chamber, below the storage assembly, and is used to heat the reaction products inside the reaction chamber.

[0011] Preferably, the placement assembly includes a top ring, a base, a vertical shaft, and a fixing member. The top ring is located in the middle of the rotating shaft and is provided with a fixing member for limiting the position of the reaction column. The bottom of the top ring is connected to the base through the vertical shaft, and the top of the base has a placement groove that fits against the outer wall of the bottom end of the reaction column.

[0012] Preferably, the fastener includes a nut block, a screw, and a handle. The nut block is integrally formed on the outer side of the top ring, and the screw is internally threaded onto the nut block. One end of the screw extends to the inner side of the top ring and abuts against the outer wall of the reaction column, and the other end of the screw is connected to a handle.

[0013] Preferably, the swing assembly includes a T-shaped frame, a guide, a toothed rack, gears, and a reciprocating drive. The outer wall of the reaction chamber is slidably connected to the T-shaped frame via the guide. A toothed rack is provided on the horizontal section of the T-shaped frame. One end of multiple rotating shafts passes through the reaction chamber and is connected to gears. Multiple gears are meshed with the toothed rack. A reciprocating drive for driving the T-shaped frame to move back and forth is also provided on the outside of the reaction chamber.

[0014] Preferably, the guide includes a guide frame and a connecting sleeve. The guide frame is connected to the top and bottom of the outer wall of the reaction chamber. The connecting sleeve is slidably sleeved on the guide frame and installed on the back of the T-shaped frame.

[0015] Preferably, the reciprocating drive includes a motor, a turntable, a pusher, and a positioning component. The motor is installed on the outer wall of the reaction chamber, and the output end of the motor is connected to the turntable. The eccentric part of the turntable end face is connected to the pusher through the positioning component. A through slot is opened on the vertical section of the T-shaped frame, and one end of the pusher extends into the through slot.

[0016] Preferably, the positioning component includes a threaded pin, and multiple sets of threaded grooves are linearly and equidistantly formed on the turntable. A through hole is formed in the middle of the push column, and the shank of the threaded pin passes through the through hole and is threadedly connected to the corresponding threaded groove.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. This utility model is equipped with a reciprocating drive component that works in conjunction with a T-shaped frame. Under the combined action of the motor, turntable, and push column, the T-shaped frame can drive the toothed column to move continuously laterally and reciprocally. The toothed column meshes with the gear, thereby driving multiple reaction columns to sway left and right, which helps to promote the peptide synthesis reaction, improve the reaction efficiency, and avoid the situation of frequent extension and retraction required by using an electric push rod as a power source, thus reducing energy consumption and mechanical wear.

[0019] 2. This utility model adjustably mounts the push column on the turntable using positioning components. By adjusting the position of the push column on the turntable, the reciprocating movement range of the T-shaped frame is indirectly adjusted. Thus, the swaying amplitude of the reaction column can be adjusted according to actual needs using the mechanical structure. It does not require a complex electronic control system to control the swaying amplitude of the reaction column, making it highly operable. Attached Figure Description

[0020] Figure 1 This is the main view of the present invention.

[0021] Figure 2 This is a side sectional view of the present invention;

[0022] Figure 3 This is a partial side view of the present invention;

[0023] Figure 4 This is a structural diagram of the protective cover of this utility model without structural details;

[0024] Figure 5 This utility model Figure 4 A partial structural diagram;

[0025] Figure 6 This utility model Figure 5 Enlarged structural diagram of part A.

[0026] In the picture:

[0027] 1. Reaction chamber; 2. Chamber cover; 3. Rotating shaft; 4. Reaction column; 5. Protective cover; 6. Maintenance door; 7. Heating wire mesh; 8. Top ring; 9. Base; 10. Vertical shaft; 11. Placement slot; 12. Nut block; 13. Screw; 14. Handle; 15. T-shaped frame; 16. Toothed rack; 17. Gear; 18. Guide frame; 19. Connecting sleeve; 20. Motor; 21. Turntable; 22. Push column; 23. Through slot; 24. Threaded pin; 25. Threaded groove. Detailed Implementation

[0028] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0029] Example 1:

[0030] As attached Figure 1 To be continued Figure 6 As shown:

[0031] This utility model provides a multi-row reaction device for polypeptide synthesis, including a reaction chamber 1, a storage assembly, and a swing assembly. A cover 2 is hinged to the reaction chamber 1. Multiple rotating shafts 3 are rotatably connected inside the reaction chamber 1. The storage assembly is located in the middle of the rotating shafts 3. A reaction column 4 is located inside the storage assembly. The swing assembly is located on the outside of the reaction chamber 1. The swing assembly is used to drive the multiple sets of rotating shafts 3 to rotate and drive the reaction column 4 to swing. A protective cover 5 installed on the outer wall of the reaction chamber 1 is provided on the outside of the swing assembly. A maintenance door 6 is hinged on the protective cover 5. An electric heating wire mesh 7 is located on the inside of the reaction chamber 1 below the storage assembly. It is used to heat the reaction products inside the reaction chamber 1.

[0032] refer to Figure 2 The placement assembly includes a top ring 8, a base 9, a vertical shaft 10, and a fixing member. The top ring 8 is located in the middle of the rotating shaft 3 and is provided with a fixing member for limiting the reaction column 4. The bottom of the top ring 8 is connected to the base 9 through the vertical shaft 10. The top of the base 9 has a placement groove 11 that fits against the outer wall of the bottom end of the reaction column 4.

[0033] refer to Figure 2 The fasteners include a nut block 12, a screw 13, and a handle 14. The nut block 12 is integrally formed on the outer side of the top ring 8. The screw 13 is internally threaded onto the nut block 12. One end of the screw 13 extends to the inner side of the top ring 8 and abuts against the outer wall of the reaction column 4. The other end of the screw 13 is connected to the handle 14.

[0034] In actual operation, the sealed reaction column 4 is placed between the base 9 and the top ring 8, and the handle 14 is rotated. The handle 14 drives the screw 13 to rotate inward relative to the top ring 8 in cooperation with the nut block 12, thereby fixing the reaction column 4 and preventing it from colliding with the placement component when shaking.

[0035] refer to Figure 3 and Figure 4 The swing assembly includes a T-shaped frame 15, a guide, a toothed rack 16, a gear 17, and a reciprocating drive. The outer wall of the reaction chamber 1 is slidably connected to the T-shaped frame 15 through the guide. The toothed rack 16 is provided on the horizontal section of the T-shaped frame 15. One end of multiple rotating shafts 3 passes through the reaction chamber 1 and is connected to the gear 17. All gears 17 are meshed with the toothed rack 16. The outer side of the reaction chamber 1 is also provided with a reciprocating drive for driving the T-shaped frame 15 to move back and forth.

[0036] During operation, the T-shaped frame 15 is driven to move laterally and reciprocally under the limit of the guide frame 18 by the reciprocating drive component. During the movement of the T-shaped frame 15, it drives the toothed rack 16 to move synchronously. When the toothed rack 16 moves, it meshes with the gear 17. The meshing force drives the placement component and the reaction column 4 to swing left and right through the rotating shaft 3.

[0037] refer to Figure 4The guide includes a guide frame 18 and a connecting sleeve 19. The top and bottom of the outer wall of the reaction chamber 1 are connected to the guide frame 18, and the connecting sleeve 19 is slidably sleeved on the guide frame 18. The connecting sleeve 19 is installed on the back of the T-shaped frame 15.

[0038] The guide frame 18 and the connecting sleeve 19 work together to limit the movement trajectory of the T-shaped frame 15, so that the connecting sleeve 19 can move steadily along the guide frame 18 during the movement.

[0039] Example 2:

[0040] As attached Figure 1 To be continued Figure 6 As shown:

[0041] This invention provides a multi-row reaction device for polypeptide synthesis. Based on the above embodiments, this embodiment also details the specific components of the reciprocating drive and its driving method for the T-shaped frame 15.

[0042] refer to Figure 3 and Figure 4 The reciprocating drive includes a motor 20, a turntable 21, a pusher 22, and a positioning component. The motor 20 is installed on the outer wall of the reaction chamber 1. The output end of the motor 20 is connected to the turntable 21. The eccentric part of the end face of the turntable 21 is connected to the pusher 22 through the positioning component. A through groove 23 is opened on the vertical section of the T-shaped frame 15. One end of the pusher 22 extends into the through groove 23.

[0043] During operation, the motor 20 drives the turntable 21 to rotate, which in turn drives the push column 22 to make a circular motion. While the push column 22 is moving in a circular motion, it pushes the T-shaped frame 15 to move laterally back and forth.

[0044] refer to Figure 5 and Figure 6 The positioning component includes a threaded pin 24. Multiple sets of threaded grooves 25 are linearly and equidistantly opened on the turntable 21. A through hole is opened in the middle of the push column 22. The rod of the threaded pin 24 passes through the through hole and is threadedly connected to the corresponding threaded groove 25.

[0045] By connecting the push column 22 to the threaded grooves 25 at different positions using the threaded pin 24, the position of the push column 22 can be adjusted, thereby adjusting the circumferential movement range of the push column 22, which in turn adjusts the lateral movement range of the T-shaped frame 15.

[0046] Working principle: The sealed reaction column 4 is inserted between the top ring 8 and the base 9, and then fixed with fasteners. The sealing cover is then closed, the heating wire mesh 7 is opened, and the motor 20 is started. The motor 20 drives the push column 22 to make a circular motion through the turntable 21. When the push column 22 moves, it drives the T-shaped frame 15 to move laterally back and forth on the guide frame 18. When the T-shaped frame 15 moves, it drives multiple rotating shafts 3 to rotate back and forth through the meshing of the gear 16 and the gear 17, thereby shaking the reaction column 4 and improving its reaction efficiency. When it is necessary to adjust the shaking amplitude of the reaction column 4, the maintenance door 6 is opened, the threaded pin 24 is unscrewed with a tool, and then it is fixed on the outside of the threaded groove 25 in another position. At this time, when the turntable 21 rotates, the circumferential range of the push column 22 changes, and the reciprocating movement range of the T-shaped frame 15 changes synchronously, thus adjusting the shaking amplitude of the reaction column 4. The whole process does not require a complex electronic control system to control the shaking amplitude of the reaction column 4, making it highly operable.

[0047] The embodiments of this utility model are given for the purpose of illustration and description. Although embodiments of this utility model have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the utility model. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this utility model, which is defined by the appended claims and their equivalents.

Claims

1. A multi-row reaction apparatus for polypeptide synthesis, characterized in that, include: The reaction chamber (1) is hinged with a cover (2) and has multiple rotating shafts (3) inside. A storage assembly is provided in the middle of the rotating shaft (3), and a reaction column (4) is provided inside the storage assembly. A swing assembly, located outside the reaction chamber (1), is used to drive multiple sets of rotating shafts (3) to rotate and cause the reaction column (4) to swing. It is covered by a protective cover (5) installed on the outer wall of the reaction chamber (1), and a maintenance door (6) is hinged to the protective cover (5); and The electric heating wire mesh (7) is located inside the reaction chamber (1) below the storage assembly and is used to heat the reaction products inside the reaction chamber (1).

2. The multi-row reaction apparatus for polypeptide synthesis as described in claim 1, characterized in that: The placement assembly includes a top ring (8), a base (9), a vertical shaft (10), and a fixing member. The top ring (8) is located in the middle of the rotating shaft (3), and a fixing member is provided on it for limiting the reaction column (4). The bottom of the top ring (8) is connected to the base (9) through the vertical shaft (10). The top of the base (9) has a placement groove (11) that fits against the outer wall of the bottom end of the reaction column (4).

3. The multi-row reaction apparatus for polypeptide synthesis as described in claim 2, characterized in that: The fasteners include a nut block (12), a screw (13) and a handle (14). The nut block (12) is integrally formed on the outer side of the top ring (8). The screw (13) is internally threaded onto the nut block (12). One end of the screw (13) extends to the inner side of the top ring (8) and abuts against the outer wall of the reaction column (4). The other end of the screw (13) is connected to the handle (14).

4. The multi-row reaction apparatus for polypeptide synthesis as described in claim 1, characterized in that: The swing assembly includes a T-shaped frame (15), a guide, a toothed rack (16), a gear (17), and a reciprocating drive. The outer wall of the reaction chamber (1) is slidably connected to the T-shaped frame (15) through the guide. A toothed rack (16) is provided on the horizontal section of the T-shaped frame (15). One end of multiple rotating shafts (3) passes through the reaction chamber (1) and is connected to a gear (17). All gears (17) are meshed with the toothed rack (16). A reciprocating drive for driving the T-shaped frame (15) to move back and forth is also provided on the outside of the reaction chamber (1).

5. The multi-row reaction apparatus for polypeptide synthesis as described in claim 4, characterized in that: The guide includes a guide frame (18) and a connecting sleeve (19). The top and bottom of the outer wall of the reaction chamber (1) are connected to the guide frame (18). The connecting sleeve (19) is slidably sleeved on the guide frame (18). The connecting sleeve (19) is installed on the back of the T-shaped frame (15).

6. The multi-row reaction apparatus for polypeptide synthesis as described in claim 4, characterized in that: The reciprocating drive includes a motor (20), a turntable (21), a pusher (22), and a positioning component. The motor (20) is installed on the outer wall of the reaction chamber (1). The output end of the motor (20) is connected to the turntable (21). The eccentric part of the end face of the turntable (21) is connected to the pusher (22) through the positioning component. A through groove (23) is opened on the vertical section of the T-shaped frame (15). One end of the pusher (22) extends into the through groove (23).

7. The multi-row reaction apparatus for polypeptide synthesis as described in claim 6, characterized in that: The positioning component includes a threaded pin (24), and multiple sets of threaded grooves (25) are linearly and equidistantly opened on the turntable (21). A through hole is opened in the middle of the push column (22), and the rod of the threaded pin (24) passes through the through hole and is threadedly connected to the corresponding threaded groove (25).

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