Nucleic acid amplification detection device

Abstract

The utility model belongs to the technical field of enzymology or microbiology devices, and particularly relates to a nucleic acid amplification detection device, which comprises a shell, a plurality of detection devices, a plurality of detection devices and a control device, the shell is internally provided with a bearing frame, the bearing frame is provided with a plurality of limiting grooves for placing test tubes, and the lower end part of each limiting groove is provided with a light inlet hole and a light outlet hole; the lamp panel is located on the outer side of the light inlet hole, at least one light emitting source is arranged on the lamp panel, and the light emitting source is suitable for emitting exciting light to the test tube in the limiting groove through the corresponding light inlet hole so as to induce a reagent in the test tube to emit a fluorescence signal; the shading assembly is arranged on the outer side of the light outlet hole; according to the nucleic acid amplification detection device, the shading assembly is arranged on one side of the bearing frame, the periphery of the fluorescent signal receiving module is covered with the shading assembly, and therefore the fluorescent signal receiving module is isolated from natural light; and meanwhile, the formed light path channels are mutually independent, so that the interference of mutual optical crosstalk is reduced.

Description

Technical Field

[0001] This invention belongs to the field of enzymology or microbiology device technology, specifically relating to a nucleic acid amplification and detection device. Background Technology

[0002] Nucleic acid amplification detection is a method that uses enzymes to amplify the nucleic acid sequence to be tested, and then detects it.

[0003] In related technologies, when nucleic acid amplification detection devices are used outdoors, the fluorescence signal intensity of the reagents is very weak compared to natural light, which greatly interferes with the detection components and ultimately affects the result determination.

[0004] Therefore, how to solve the technical problem of natural light interfering with the detection components when used outdoors is a problem that urgently needs to be solved by those skilled in the art.

[0005] It should be noted that the information disclosed in this background section is only for understanding the background technology of the present application concept, and therefore, the above description is not considered to constitute prior art information. Utility Model Content

[0006] This disclosure provides at least one nucleic acid amplification detection device.

[0007] This disclosure provides a nucleic acid amplification detection device, comprising: a shell containing a support frame, the support frame having a plurality of limiting grooves for placing test tubes, the lower end of each limiting groove having a light inlet and a light outlet; a lamp plate located outside the light inlet, the lamp plate having at least one light source, the light source being adapted to emit excitation light into the test tubes in the limiting grooves through corresponding light inlets to induce the reagents in the test tubes to emit fluorescence signals; a light-shielding assembly located outside the light outlet; and a fluorescence signal receiving module located inside the light-shielding assembly, having a plurality of receiving sources thereon, each receiving source being adapted to receive the fluorescence signal emitted from a corresponding light outlet.

[0008] In one optional embodiment, a reflector is provided inside the support frame, and the reflector and the light-emitting hole are located on opposite sides of the limiting groove; wherein the reflector is adapted to guide the fluorescent signal emitted by the reagent into the light-emitting hole.

[0009] In one alternative embodiment, the bottom of the support frame has mounting holes for embedding a temperature sensor.

[0010] In one optional embodiment, the light-shielding assembly includes: a light-shielding housing and a light-shielding housing cover; wherein a first retainer is disposed within the light-shielding housing, and a light-shielding chamber is formed between the first retainer, the light-shielding housing, and the light-shielding housing cover; and the fluorescence signal receiving module is disposed within the light-shielding chamber.

[0011] In one optional embodiment, the first holder has a plurality of first isolation slots on the side facing the fluorescence signal receiving module, and the bottom of the first isolation slot has a first light-passing hole; wherein each of the receiving sources is respectively disposed in the corresponding first isolation slot; the first light-passing hole and the light-emitting hole are coaxially arranged.

[0012] In one alternative embodiment, the first retainer has a first mounting groove on the side facing the support frame; a first filter is disposed in the first mounting groove.

[0013] In one optional embodiment, a second retainer is provided on the outer side of the light inlet hole; a plurality of second isolation grooves are provided on the side of the second retainer away from the support frame, and a second light-passing hole is provided at the bottom of the second isolation groove; wherein each of the light sources is respectively disposed in the corresponding second isolation groove; the second light-passing hole is coaxially arranged with the light inlet hole.

[0014] In one alternative embodiment, the second retainer has a second mounting groove on the side facing the support frame; a second filter is disposed in the second mounting groove.

[0015] In one optional embodiment, the outer casing is provided with a flip cover; the inner side of the flip cover is provided with a sealing cover; when the flip cover is closed with the outer casing, the sealing cover is adapted to cover the test tube protruding from the limiting groove to block light.

[0016] In one optional embodiment, a second retainer is provided on the outer side of the light inlet hole; wherein a sliding groove is formed on the side of the second retainer away from the support frame, and a plurality of second light-passing holes coaxial with the corresponding light inlet holes are formed in the sliding groove; a second mounting groove is formed on the side of the second retainer facing the support frame, and a second filter is provided in the second mounting groove; the lamp plate is slidably disposed in the sliding groove, and a light source is provided on the lamp plate; a light-shielding sleeve is fitted around the light source; the lamp plate is disposed on a slider, and the slider is disposed on a lead screw; one end of the lead screw is connected to a servo motor; wherein the servo motor is connected to a control module, and the control module is configured to control the servo motor to drive the lead screw to rotate, so that the light source on the lamp plate moves between the second light-passing holes.

[0017] The beneficial effects of this utility model are that, by setting a light-shielding component on one side of the support frame, the light-shielding component covers the periphery of the fluorescence signal receiving module, thereby isolating the fluorescence signal receiving module from natural light; at the same time, the formed optical paths are independent of each other, reducing interference from cross-lighting.

[0018] Other features and advantages of this invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objectives and other advantages of this invention are realized and obtained through the structures particularly pointed out in the description and the accompanying drawings.

[0019] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, preferred embodiments are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0020] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0021] Figure 1 A perspective view of a nucleic acid amplification and detection device provided in an embodiment of this disclosure;

[0022] Figure 2 This is a schematic cross-sectional view of a nucleic acid amplification and detection device provided in an embodiment of the present disclosure;

[0023] Figure 3 A schematic diagram of the mounting position structure of a reflector provided in an embodiment of this disclosure;

[0024] Figure 4 This is a schematic diagram of a support frame provided in an embodiment of the present disclosure;

[0025] Figure 5 This is a schematic diagram of the position structure of a mounting hole provided in an embodiment of the present disclosure;

[0026] Figure 6 This is a schematic diagram of the structure of a light-shielding component provided in an embodiment of the present disclosure;

[0027] Figure 7 This is a schematic diagram of the structure of a first cage provided in an embodiment of the present disclosure;

[0028] Figure 8 A cross-sectional view of a first cage provided in an embodiment of this disclosure;

[0029] Figure 9 A cross-sectional view of a second cage provided in an embodiment of this disclosure;

[0030] Figure 10 This is a schematic diagram of a flip cover structure provided in an embodiment of the present disclosure;

[0031] Figure 11 This is a schematic diagram of the structure of a single light source provided in an embodiment of the present disclosure;

[0032] Figure 12 This is a schematic diagram of the driving structure of a lamp board with a single light source, provided in an embodiment of this disclosure.

[0033] In the picture:

[0034] 1. Outer shell, 11. Support frame, 12. Limiting groove, 13. Light outlet hole, 14. Light inlet hole, 15. Flip cover, 16. Sealing cover, 17. Mounting hole;

[0035] 2. Light panel; 21. Light source; 22. Reflector; 23. Light shield;

[0036] 3. Light-shielding component; 31. Light-shielding housing; 32. Light-shielding housing cover; 33. Light-shielding chamber;

[0037] Fluorescence signal receiving module 4, receiving source 41;

[0038] First retainer 5, first isolation groove 51, first light-passing hole 52, first mounting groove 53, first filter 54;

[0039] Second retainer 6, second isolation groove 61, second light passage 62, second mounting groove 63, second filter 64, slide groove 65;

[0040] Test tube 7, reagent 71;

[0041] Slider 81, lead screw 82, servo motor 83. Detailed Implementation

[0042] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0043] It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures. Furthermore, in the figures, the thickness of parts may be exaggerated or reduced for the purpose of effectively depicting the technical content.

[0044] The following detailed description, with reference to the accompanying drawings, describes some embodiments of the present invention. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0045] like Figures 1 to 3 As shown, at least one embodiment provides a nucleic acid amplification detection device, including: a shell 1 and a support frame 11, a lamp plate 2, and a fluorescence signal receiving module 4 disposed within the shell 1. The support frame 11 is used to place test tubes 7 to be tested. The lamp plate 2 emits excitation light to the reagents in the test tubes 7 to induce the reagents 71 in the test tubes 7 to emit fluorescence signals. Finally, the fluorescence signals are acquired by the receiving sources 41 of the fluorescence signal receiving module 4.

[0046] In this embodiment, reagent 71 contains a fluorescent group. When excitation light irradiates reagent 71, the excitation light induces the fluorescent group to emit a fluorescent signal, which is then collected by the receiving source 41 of the fluorescence signal receiving module 4 to complete the detection requirement. The working principle of the fluorescence signal receiving module 4 is the prior art and will not be improved.

[0047] like Figure 4 As shown, in some embodiments, the support frame 11 is provided with a plurality of limiting grooves 12 for placing test tubes 7.

[0048] like Figure 2 As shown, in some embodiments, a light inlet hole 14 and a light outlet hole 13 are provided at the lower end of the limiting groove 12.

[0049] In this embodiment, the light source 21 on the lamp plate 2 emits excitation light into the test tube 7 in the limiting groove 12 through the light inlet hole 14. The reagent 71 in the test tube 7 emits a fluorescence signal that is emitted from the light outlet hole 13 and collected by the receiving source 41 of the fluorescence signal receiving module 4. The limiting grooves 12 are not interconnected, thereby preventing the fluorescence signals in the test tubes 7 from affecting each other.

[0050] like Figure 2 As shown, in some embodiments, the lamp panel 2 is located outside the light inlet hole 14, and at least one light source 21 is provided on the lamp panel 2.

[0051] In one application scenario, the number of light sources 21 on the light panel 2 is the same as the number of limiting slots 12, with one light source 21 corresponding to one limiting slot 12; wherein, the light source 21 may, but is not limited to, using LED monochrome lamp beads.

[0052] In related technologies, when nucleic acid amplification detection devices are used outdoors, the fluorescence intensity emitted by reagent 71 is very weak compared to natural light, which greatly interferes with the detection components (i.e., receiver 41) and ultimately affects the result determination.

[0053] To address the aforementioned issues, in some embodiments, a light-shielding component 3 is provided on the outside of the light-emitting hole 13. The light-shielding component 3 covers the periphery of the fluorescence signal receiving module 4, thereby isolating the fluorescence signal receiving module 4 from natural light and preventing interference from natural light to the fluorescence signal receiving module 4.

[0054] In this embodiment, the fluorescence signal receiving module is provided with a plurality of receiving sources 41, the number of receiving sources 41 being the same as the number of limiting grooves 12, and one receiving source 41 receiving the fluorescence signal emitted by the reagent 71 in one limiting groove 12.

[0055] like Figure 6 As shown, specifically, the light-shielding component 3 includes: a light-shielding housing 31 and a light-shielding housing cover 32; wherein a first retainer 5 is provided inside the light-shielding housing 31, and a light-shielding chamber 33 is formed between the first retainer 5, the light-shielding housing 31 and the light-shielding housing cover 32; and a fluorescence signal receiving module 4 is disposed inside the light-shielding chamber 33.

[0056] In this embodiment, the light-shielding housing 31 and the light-shielding housing cover 32 may be, but are not limited to, using light-shielding engineering plastics; the light-shielding housing 31 is set close to the support frame 2, thereby preventing natural light from entering the light-shielding chamber 33; the light-shielding chamber 33 provides a light-shielding and enclosed environment for the fluorescent signal receiving module 4, avoiding interference from ambient natural light.

[0057] like Figure 9 As shown, in some embodiments, a second retainer 6 is provided on the outer side of the light inlet 14; a plurality of second isolation grooves 61 are provided on the side of the second retainer 6 away from the support frame 11, and a second light-passing hole 62 is provided at the bottom of the second isolation groove 61; wherein each light source 21 is respectively disposed in the corresponding second isolation groove 61; the second light-passing hole 62 is coaxially disposed with the light inlet 14.

[0058] In this embodiment, the second isolation slots 61 are not interconnected, thereby reducing crosstalk interference between them.

[0059] like Figure 9 As shown, in some embodiments, the second retainer 6 has a second mounting groove 63 on the side facing the support frame 11; a second filter 64 is disposed in the second mounting groove 63.

[0060] In this embodiment, the LED monochromatic lamp bead is used as the excitation light, with a center wavelength of 470nm or 530nm. The center wavelength of the second filter 64 is also 470nm or 530nm, thereby filtering out unwanted spectral components in the excitation light. After the excitation light passes through the second light-passing hole 62, the second filter 64, and the light-entry hole 14 in sequence, it irradiates the reagent 71, thereby inducing the reagent 71 to emit a fluorescence signal.

[0061] like Figure 6 , Figure 7 As shown, in some embodiments, the first retainer 5 has a plurality of first isolation grooves 51 on the side facing the fluorescent signal receiving module 4, and a first light-passing hole 52 is provided at the bottom of the first isolation groove 51; wherein each receiving source 41 is respectively arranged in the corresponding first isolation groove 51; the first light-passing hole 52 is coaxially arranged with the light-emitting hole 13.

[0062] In this embodiment, the first isolation slots 51 are not interconnected, thereby reducing crosstalk interference between them.

[0063] like Figure 8 As shown, in some embodiments, the first retainer 5 has a first mounting groove 53 on the side facing the support frame 11; a first filter 54 is disposed in the first mounting groove 53.

[0064] In this embodiment, the fluorescence signal emitted by reagent 71 is collected by receiving source 41 after passing through light-emitting hole 13, first filter 54, and first light-passing hole 52 in sequence; wherein, when passing through the first filter 54, the first filter 54 will filter out unwanted spectral components.

[0065] In some embodiments, the excitation light in the second isolation groove 61 passes sequentially through the second light-passing hole 62, the second filter 64, and the light-entry hole 14, and then irradiates the reagent 71, thereby inducing the reagent 71 to emit a fluorescence signal. The fluorescence signal emitted by the reagent 71 passes sequentially through the light-exiting hole 13, the first filter 54, and the first light-passing hole 52, and is then collected by the receiving source 41 in the first isolation groove 51. The second isolation groove 61, the second light-passing hole 62, the second filter 64, the light-entry hole 14, the limiting groove 12, the light-exiting hole 13, the first filter 54, the first light-passing hole 52, and the first isolation groove 51 form an optical path channel. The components of the optical path channel are sealed with silicone sealing rings to reduce cross-light interference between the holes.

[0066] In related technologies, the fluorescent signal emitted by reagent 71 is emitted in all directions, resulting in low detection sensitivity.

[0067] To solve the above problems, such as Figure 2 As shown, in some embodiments, a reflector 22 is provided inside the support frame 11, and the reflector 22 and the light emission hole 13 are located on both sides of the limiting groove 12; wherein the reflector 22 is adapted to guide the fluorescent signal emitted by the reagent into the light emission hole 13.

[0068] In this embodiment, a portion of the fluorescence signal emitted by the reagent 71 is emitted by the reflector 22, allowing more fluorescence signals to enter the light outlet 13 and be collected by the receiving source 41, thereby further improving the detection sensitivity.

[0069] like Figure 5As shown, in some embodiments, the bottom of the support frame 11 has a mounting hole 17 for embedding a temperature sensor.

[0070] In this embodiment, after the temperature sensor is embedded in the mounting hole 17, the mounting hole 17 is filled with thermally conductive silicone grease. Embedding the temperature sensor inside the support frame 11 can reduce the influence of external ambient air heat convection exchange, accurately measure the temperature of the support frame 11, and improve temperature control accuracy.

[0071] like Figure 10 As shown, in some embodiments, a flip cover 15 is provided on the outer shell 1; a sealing cover 16 is provided on the inner side of the flip cover 15; when the flip cover 15 is closed with the outer shell 1, the sealing cover 16 is adapted to cover the test tube 7 protruding from the limiting groove 12 to block light.

[0072] In this embodiment, the sealing cover 16 may be, but is not limited to, a silicone sealing cover. When the flip cover 15 is closed with the outer shell 1, the sealing cover 16 abuts against the support frame 11, so that the upper part of the test tube 7 is blocked from light.

[0073] In another application scenario, such as Figure 11 , Figure 12 As shown, a second retainer 6 is provided on the outer side of the light inlet 14; a sliding groove 65 is provided on the side of the second retainer 6 away from the support frame 11, and a plurality of second light-passing holes 62 coaxial with the corresponding light inlet 14 are provided in the sliding groove 65. A second mounting groove 63 is provided on the side of the second retainer 6 facing the support frame 11, and a second filter 64 is provided in the second mounting groove 63; the lamp plate 2 is slidably disposed in the sliding groove 65, and a light source 21 is provided on the lamp plate 2. A light-shielding sleeve 23 is provided around the light source 21. The lamp plate 2 is disposed on a slider 81, and the slider 81 is disposed on a lead screw 82. One end of the lead screw 82 is connected to a servo motor 83; the servo motor 83 is connected to a control module, and the control module is configured to control the servo motor 83 to drive the lead screw 82 to rotate, so that the light source 21 on the lamp plate 2 moves between the second light-passing holes 62.

[0074] In this embodiment, the control module may be, but is not limited to, a PLC; two buttons connected to the control module may be provided on the housing 1. By pressing the buttons, the forward and reverse rotation of the servo motor 83 is controlled, thereby driving the lamp plate 2 to move within the slide groove 65. During the movement of the lamp plate 2, the light source 21 does not work to prevent it from affecting the reagent 71 in other limiting grooves 12. This embodiment uses a single light source 21 to ensure the uniformity of the light source and avoid the difference between multiple light sources 21 affecting the detection effect.

[0075] In summary, this nucleic acid amplification detection device isolates the fluorescence signal receiving module 4 from natural light by providing a light-shielding component 3 on one side of the support frame 2, thereby covering the fluorescence signal receiving module 4. At the same time, the formed optical paths are independent of each other, reducing crosstalk interference.

[0076] In this document, when it is said that the first component is located on the second component, this can mean that the first component can be directly formed on the second component, or that the third component can be inserted between the first component and the second component.

[0077] In this document, when an element or layer is referred to as “located,” “joined to,” “connected to,” “attached to,” or “coupled to” another element or layer, it may be directly located, joined, connected, attached to, or coupled to the other element or layer, or there may be intermediate elements or layers present. Conversely, when an element is referred to as “directly on another element or layer,” “directly joined to,” “directly connected to,” “directly attached to,” or “directly coupled to” another element or layer, there may be no intermediate elements or layers present. Other terms used to describe relationships between elements should be interpreted in a similar manner (e.g., “between” versus “directly between,” “adjacent” versus “directly adjacent,” etc.). As used herein, the term “and / or” includes any and all combinations of one or more of the related listed items.

[0078] In this document, exemplary embodiments of the present disclosure will be described in more detail with reference to the accompanying drawings. As used herein, expressions such as “at least one of…” modify the entire list of elements when following a list of elements, rather than individual elements in the list. For example, the expression “at least one of a, b, and c” should be understood to include only a, only b, only c, both a and b, both a and c, both b and c, or all of a, b, and c.

[0079] The terminology used herein is for the purpose of describing specific exemplary configurations only and is not intended to be limiting. As used herein, the singular articles “a,” “an,” and “the” may also be intended to include plural forms unless otherwise clearly stated herein. The terms “comprising,” “including,” and “having” are inclusive and thus specify the presence of features, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, steps, operations, elements, components, and / or combinations thereof. The method steps, processes, and operations described herein should not be construed as requiring them to be performed in the specific order discussed or shown, unless specifically identified as such. Additional or alternative steps may be employed.

[0080] As used herein, the phrases “in one embodiment,” “according to one embodiment,” “in some embodiments,” etc., generally refer to the fact that a particular feature, structure, or characteristic following the phrase can be included in at least one embodiment of this disclosure. Therefore, a particular feature, structure, or characteristic can be included in more than one embodiment of this disclosure, such that these phrases do not necessarily refer to the same embodiment. As used herein, the terms “example,” “exemplary,” etc., are used to “serve as an example, instance, or illustration.” Any implementation, aspect, or design described herein as “example” or “exemplary” is not necessarily to be construed as preferred or superior to other implementations, aspects, or designs. Rather, the use of the terms “example,” “exemplary,” etc., is intended to present concepts in a specific manner.

[0081] In the description of the embodiments of this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0082] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, terms such as "first," "second," and other numerical terms used herein do not imply order or sequence unless expressly indicated herein. Therefore, without departing from the teachings of the exemplary embodiments, the first element, component, region, layer, or segment discussed above may be referred to as the second element, component, region, layer, or segment.

[0083] Spatially relative terms, such as “inside,” “outside,” “below,” “below,” “down,” “above,” “up,” etc., may be used herein to describe the relationship between one element or feature illustrated in the figures and another element or feature. In addition to the orientations depicted in the figures, spatially relative terms may be intended to cover different orientations of the device in use or operation. For example, if the device in the figure is flipped, an element described as “below” or “below” other elements or features would be oriented as “above” other elements or features. Thus, the example term “below” can cover both above and below orientations. The device may be oriented in other ways (rotated 90 degrees or in other orientations), and the spatially relative descriptors used herein are interpreted accordingly.

[0084] In the above discussion, unless otherwise stated, when used to describe numerical values, the terms “about,” “approximately,” “basically,” etc., indicate a change of + / - 10% in that value.

[0085] Based on the above-described preferred embodiments of this utility model, and through the foregoing description, those skilled in the art can make various changes and modifications without departing from the technical concept of this utility model. The technical scope of this utility model is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. A nucleic acid amplification and detection device, characterized in that, include: The outer shell (1) has a support frame (11) inside. The support frame (11) has several limiting grooves (12) for placing test tubes (7). The lower end of the limiting groove (12) has a light inlet hole (14) and a light outlet hole (13). The lamp plate (2) is located outside the light inlet hole (14). At least one light source (21) is provided on the lamp plate (2). The light source (21) is adapted to emit excitation light into the test tube (7) in the limiting groove (12) through the corresponding light inlet hole (14) to induce the reagent (71) in the test tube (7) to emit a fluorescent signal. A light-shielding component (3) is disposed on the outside of the light-emitting hole (13); The fluorescence signal receiving module (4) is located inside the light-shielding component (3) and is provided with a plurality of receiving sources (41). Each of the receiving sources (41) is adapted to receive the fluorescence signal emitted from the corresponding light-emitting hole (13).

2. The nucleic acid amplification and detection device as described in claim 1, characterized in that, A reflector (22) is provided inside the support frame (11), and the reflector (22) and the light outlet (13) are located on opposite sides of the limiting groove (12); wherein The reflector (22) is adapted to guide the fluorescent signal emitted by the reagent into the light outlet (13).

3. The nucleic acid amplification and detection device as described in claim 1, characterized in that, The bottom of the support frame (11) is provided with a mounting hole (17) for embedding a temperature sensor.

4. The nucleic acid amplification and detection device as described in claim 1, characterized in that, The light-shielding component (3) includes: a light-shielding housing (31) and a light-shielding housing cover (32); wherein A first retainer (5) is provided inside the light-shielding housing (31), and a light-shielding chamber (33) is formed between the first retainer (5), the light-shielding housing (31) and the light-shielding housing cover (32). The fluorescence signal receiving module (4) is located inside the light-shielding chamber (33).

5. The nucleic acid amplification and detection device as described in claim 4, characterized in that, The first retainer (5) has a plurality of first isolation grooves (51) on the side facing the fluorescence signal receiving module (4), and a first light-passing hole (52) is formed at the bottom of the first isolation groove (51); wherein Each of the receiving sources (41) is respectively disposed in the corresponding first isolation slot (51); The first light-passing aperture (52) and the light-exiting aperture (13) are coaxially arranged.

6. The nucleic acid amplification and detection device as described in claim 5, characterized in that, The first retainer (5) has a first mounting groove (53) on the side facing the support frame (11); A first filter (54) is provided in the first mounting slot (53).

7. The nucleic acid amplification and detection device as described in claim 1, characterized in that, A second retainer (6) is provided on the outside of the light inlet (14). The second retainer (6) has a plurality of second isolation grooves (61) on the side away from the support frame (11), and the bottom of the second isolation grooves (61) has a second light-passing hole (62); wherein Each of the light sources (21) is respectively disposed in the corresponding second isolation groove (61); The second light-passing hole (62) is coaxially arranged with the light-entry hole (14).

8. The nucleic acid amplification and detection device as described in claim 7, characterized in that, The second retainer (6) has a second mounting groove (63) on the side facing the support frame (11); A second filter (64) is provided in the second mounting slot (63).

9. The nucleic acid amplification and detection device as described in claim 1, characterized in that, The outer casing (1) is provided with a flip cover (15); A sealing cover (16) is provided on the inner side of the flip cover (15). When the flip cover (15) is closed with the outer shell (1), the sealing cover (16) is adapted to cover the test tube (7) protruding from the limiting groove (12) to block light.

10. The nucleic acid amplification and detection device as described in claim 1, characterized in that, A second retainer (6) is provided on the outer side of the light inlet (14); wherein The second retainer (6) has a sliding groove (65) on the side away from the support frame (11), and a plurality of second light-passing holes (62) coaxial with the corresponding light-entry holes (14) are provided in the sliding groove (65). The second retainer (6) has a second mounting groove (63) on the side facing the support frame (11), and a second filter (64) is provided in the second mounting groove (63). The lamp plate (2) is slidably disposed in the slide groove (65). A light source (21) is disposed on the lamp plate (2). A light shield (23) is fitted around the light source (21). The lamp plate (2) is disposed on a slider (81). The slider (81) is disposed on a lead screw (82). One end of the lead screw (82) is connected to a servo motor (83). The servo motor (83) is connected to the control module, which is configured to control the servo motor (83) to drive the lead screw (82) to rotate so that the light source (21) on the lamp board (2) moves between each of the second light holes (62).

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