Ultrasound mediated gene transfection device

Abstract

The utility model discloses an ultrasound mediation gene transfection device which comprises a transfection box, a supporting partition plate is fixedly connected to the lower end of an inner cavity of the transfection box, an ultrasonic generator is fixedly installed at the middle end of the bottom of the supporting partition plate, a through hole is formed in the surface of the supporting partition plate, and a transfection container is movably connected to the top of the supporting partition plate. By arranging the transfection box and the supporting partition plate, a transfection container containing cell sap can be supported in the gene transfection process, and by arranging the driving motor, the first screw rod, the fixed frame, the movable frame, the movable frame, the energy converter, the through hole, the double-shaft motor, the second screw rod, the movable plate, the push rod and the ultrasonic generator, the cell sap can be supported in the transfection process. The purpose of ultrasound-mediated gene transfection is achieved, and corresponding operation can be automatically performed on cell sap in the transfection container one by one in the gene transfection process, so that the transfection operation quality is effectively ensured.

Description

Technical Field

[0001] This utility model relates to the field of gene transfection technology, specifically to an ultrasound-mediated gene transfection device. Background Technology

[0002] Ultrasound-mediated gene transfection (UMTZ) is a technique that uses ultrasound and microbubble contrast agents to promote the efficient and safe transfection of genes into cells. Ultrasound-microbubble-mediated gene transfection technology refers to the enrichment and release of ultrasound energy at the target tissue through the cavitation effect of microbubbles, thereby promoting the internalization of gene drugs by cells in that tissue and achieving the goal of efficient transfection. This technology utilizes the physical properties of ultrasound and microbubble contrast agents as carriers. Through the cavitation effect generated by ultrasound in liquids, bubbles are formed and ruptured, thereby creating transient gaps in the cell membrane, increasing cell membrane permeability, and facilitating the entry of genes and carrier solutions into the cells, thereby improving gene transfection and expression.

[0003] For example, the Chinese Utility Model Application provides "An Ultrasonic-Mediated Gene Transfection Device" (Announcement No.: CN218372344U). This application includes an operating table, an ultrasonic transfection instrument mounted on the top of the operating table, an ultrasonic probe mounted on the top of the ultrasonic transfection instrument, an operating box mounted on the side of the top of the operating table away from the ultrasonic transfection instrument, an internal cavity in the operating box, a heating tube mounted at the bottom of the cavity, a placement plate in the cavity, multiple transfection tubes mounted on the top of the placement plate, and self-rotation drive components on both sides of the placement plate. This utility model allows the placement plate to be rotated by pulling the pull block upwards and moving the round rod upwards, thereby disengaging the round rod from the fixed plate. Then, by rotating the handle, the rotating shaft is rotated, which in turn rotates the fixed frame, thereby rotating the placement plate. After rotation, by lowering the pull block, the fixed plate, under the push of the spring, drives the round rod to be inserted into the limiting groove of the rotating shaft for fixation.

[0004] The ultrasound-mediated gene transfection device described above often requires manual handling of the ultrasound probe during use, ensuring that the probe contacts the bottom of the cell culture plate. Because the distance between the transfection tube and the ultrasound probe varies during this process, the degree of cavitation effect in the liquid within each transfection tube differs, which in turn affects the quality of transfection of each group of cells, thus failing to meet the user's needs. Utility Model Content

[0005] The purpose of this invention is to provide an ultrasound-mediated gene transfection device that can operate automatically, making it convenient for personnel while effectively improving the quality of cell transfection.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an ultrasound-mediated gene transfection device, comprising a transfection chamber, a supporting partition fixedly connected to the lower end of the inner cavity of the transfection chamber, an ultrasonic generator fixedly installed at the middle of the bottom of the supporting partition, a through hole opened on the surface of the supporting partition, a transfection container movably connected to the top of the supporting partition, a drive motor fixedly installed at the lower end of the right side of the outer surface of the transfection chamber, a first screw fixedly installed at the output end of the drive motor, and the left side of the first screw movably connected to the left side of the inner cavity of the transfection chamber via a bearing. At the lower end, a fixed frame is threadedly connected to the middle end of the first screw, and a movable frame is fixedly connected to the bottom of the fixed frame. A dual-axis motor is fixedly installed at the middle end of the bottom of the movable frame. A second screw is fixedly installed on both sides of the output end of the dual-axis motor. The surface of the second screw is movably connected to the lower end of the movable frame through a bearing. A movable plate is threadedly connected to the middle end of the second screw. A push rod is movably connected to the upper end of the movable plate through a pin. A movable frame is movably connected to the upper end of the push rod through a pin. A transducer is fixedly installed on the top of the movable frame.

[0007] As a preferred embodiment, the top of the transfection box is movably connected to a protective cover plate via a hinge, a rubber block is fixedly connected to the front surface of the protective cover plate, hooks are fixedly connected to both ends of the top of the protective cover plate, buckles are fixedly connected to both ends of the front surface of the transfection box, and rubber bases are fixedly connected to the bottom of the outer surface of the transfection box around all four sides.

[0008] As a preferred embodiment, an annular positioning frame is fixedly connected to the top of the supporting partition, the lower end of the outer surface of the transfection container is movably connected to the inner cavity of the annular positioning frame, a rubber ring is fixedly connected to the inner cavity of the annular positioning frame, and the lower end of the outer surface of the transfection container is movably connected to the surface of the rubber ring.

[0009] As a preferred embodiment, heating tubes are fixedly installed at the upper ends of both sides of the inner cavity of the transfection chamber, a temperature sensor is fixedly installed at the middle end of the top of the support partition, and a temperature controller is fixedly installed at the middle end of the front surface of the transfection chamber.

[0010] As a preferred embodiment, both ends of the bottom of the movable frame are movably connected to guide crossbars, and the two sides of the guide crossbars are fixedly connected to the lower end of the inner cavity of the transfection box.

[0011] As a preferred embodiment, a fixing block is fixedly connected to the lower ends of both sides of the outer surface of the movable frame, and a support spring is fixedly connected between the top of the fixing block and the bottom of the movable frame.

[0012] As a preferred embodiment, guide slide rods are fixedly connected to both sides of the movable frame, and the surface of the movable frame is movably connected to the surface of the guide slide rods.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0014] 1. This utility model, through the setting of a transfection box and a supporting partition, can support the transfection container containing cell fluid during gene transfection. By setting up a drive motor, a first screw, a fixed frame, a moving frame, a moving bracket, a transducer, a through hole, a dual-axis motor, a second screw, a moving plate, a push rod, and an ultrasonic generator, the purpose of ultrasound-mediated gene transfection is achieved. Moreover, because the cell fluid inside the transfection container can be manipulated one by one automatically during gene transfection, the quality of the transfection operation is effectively guaranteed, while bringing great convenience to the personnel.

[0015] 2. This utility model, through the design of hooks, buckles, a protective cover, and rubber blocks, facilitates the locking of the protective cover to the transfection chamber during gene transfection. While protecting the top of the transfection chamber, the rubber blocks contact the top of the transfection container during locking, effectively limiting its movement. The annular positioning frame and rubber ring enhance the contact between the transfection container and the frame, while also effectively limiting the container's movement around the perimeter, preventing displacement along the top of the support partition. The inclusion of a heating element, temperature sensor, and temperature controller further enhances the design. This design allows personnel to regulate the internal temperature of the transfection chamber during gene transfection, facilitating effective cell incubation. The guide crossbars provide support and guidance to the moving frame, preventing tilting during movement. The fixed blocks and support springs stretch the springs as the frame moves upwards, generating tension. This tension then pulls the frame downwards as the two moving plates move closer together. The guide slides further guide the frame, preventing tilting during movement. Attached Figure Description

[0016] Figure 1 This is a perspective view of the present utility model;

[0017] Figure 2 This is a schematic diagram of the front cross-sectional structure of the transfer box of this utility model;

[0018] Figure 3 This is a schematic cross-sectional view of the left side of the transfection box of this utility model;

[0019] Figure 4 This utility model Figure 3 A magnified view of section A in the image.

[0020] In the diagram: 1. Dyeing box; 2. Rubber base; 3. Fastener; 4. Dyeing container; 5. Temperature controller; 6. Protective cover; 7. Rubber block; 8. Hook; 9. Heating tube; 10. Temperature sensor; 11. Drive motor; 12. Support partition; 13. Ultrasonic generator; 14. Fixing frame; 15. Moving frame; 16. Annular positioning frame; 17. First screw; 18. Transducer; 19. Guide slide bar; 20. Support spring; 21. Fixing block; 22. Push rod; 23. Rubber ring; 24. Dual-axis motor; 25. Second screw; 26. Through hole; 27. Moving plate; 28. Moving frame; 29. ​​Guide crossbar. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0023] Example 1:

[0024] Please see Figures 1-4As shown, this utility model provides an ultrasound-mediated gene transfection device, including a transfection chamber 1. A support partition 12 is fixedly connected to the lower end of the inner cavity of the transfection chamber 1. An ultrasonic generator 13 is fixedly installed at the middle of the bottom of the support partition 12. A through hole 26 is opened on the surface of the support partition 12. A transfection container 4 is movably connected to the top of the support partition 12. A drive motor 11 is fixedly installed at the lower end of the right side of the outer surface of the transfection chamber 1. A first screw 17 is fixedly installed at the output end of the drive motor 11. The left side of the first screw 17 is movably connected to the lower end of the left side of the inner cavity of the transfection chamber 1 through a bearing. A fixed frame 14 is threadedly connected to the middle of the 7. A movable frame 15 is fixedly connected to the bottom of the fixed frame 14. A dual-axis motor 24 is fixedly installed at the middle of the bottom of the movable frame 15. A second screw 25 is fixedly installed on both sides of the output end of the dual-axis motor 24. The surface of the second screw 25 is movably connected to the lower end of the inner cavity of the movable frame 15 through a bearing. A movable plate 27 is threadedly connected to the middle of the second screw 25. A push rod 22 is movably connected to the upper end of the movable plate 27 through a pin. A movable frame 28 is movably connected to the upper end of the push rod 22 through a pin. A transducer 18 is fixedly installed on the top of the movable frame 28.

[0025] In this technical solution, the transfection box 1 and the supporting partition 12 are used to support the transfection container 4 containing cell fluid during gene transfection. The drive motor 11, the first screw 17, the fixed frame 14, the moving frame 15, the moving frame 28, the transducer 18, the through hole 26, the dual-axis motor 24, the second screw 25, the moving plate 27, the push rod 22 and the ultrasonic generator 13 are used to achieve the purpose of ultrasound-mediated gene transfection. In addition, since the cell fluid inside the transfection container 4 can be operated one by one and automatically during the gene transfection process, the quality of the transfection operation is effectively guaranteed, and the work of personnel is greatly facilitated.

[0026] Example 2:

[0027] Based on Embodiment 1, this utility model is as follows: Figures 1-4As shown, a protective cover 6 is movably connected to the top of the transfection box 1 via a hinge. A rubber stop 7 is fixedly connected to the front surface of the protective cover 6. Hooks 8 are fixedly connected to both ends of the top of the protective cover 6. Fasteners 3 are fixedly connected to both ends of the front surface of the transfection box 1. Rubber bases 2 are fixedly connected to the bottom of the outer surface of the transfection box 1. An annular positioning frame 16 is fixedly connected to the top of the supporting partition 12. The lower end of the outer surface of the transfection container 4 is movably connected to the inner cavity of the annular positioning frame 16. A rubber ring 23 is fixedly connected to the inner cavity of the annular positioning frame 16. The lower end of the outer surface of the transfection container 4 is movably connected to the surface of the rubber ring 23. Heating tubes 9 are fixedly installed on the upper ends of both sides of the inner cavity. Temperature sensor 10 is fixedly installed at the middle of the top of the support partition 12. PLC controller 5 is fixedly installed at the middle of the front surface of the dyeing box 1. Guide crossbars 29 are movably connected to both ends of the bottom of the moving frame 15. The two sides of the guide crossbars 29 are fixedly connected to the lower end of the inner cavity of the dyeing box 1. Fixing blocks 21 are fixedly connected to the lower ends of both sides of the outer surface of the moving frame 15. Support springs 20 are fixedly connected between the top of the fixing blocks 21 and the bottom of the moving frame 28. Guide slide rods 19 are fixedly connected to both sides of the moving frame 15. The surface of the moving frame 28 is movably connected to the surface of the guide slide rods 19.

[0028] In this technical solution, the hook 8, buckle 3, protective cover 6, and rubber stop 7 facilitate the locking of the protective cover 6 to the transfection chamber 1 during gene transfection. This protects the top of the transfection chamber 1 while allowing the rubber stop 7 to contact the top of the transfection container 4 during locking, effectively limiting its movement. The annular positioning frame 16 and rubber ring 23 enhance the contact between the transfection container 4 and the annular positioning frame 16, effectively limiting the movement of the transfection container 4 around its perimeter and preventing displacement along the top of the support partition 12. The heating tube 9, temperature sensor 10, and temperature controller 5 further enhance the temperature control. During gene transfection, the personnel can regulate the internal temperature of the transfection chamber 1 to facilitate effective incubation of the cell solution. The guide bar 29 is used to support and guide the moving frame 15, preventing it from tilting during movement. The fixing block 21 and the support spring 20 are used to stretch the support spring 20 as the moving frame 28 moves upward, thus generating tension on the moving frame 28. This allows the support spring 20 to pull the moving frame 28 downward as the two sets of moving plates 27 move closer to each other. The guide slide bar 19 is used to guide the moving frame 28, preventing it from tilting during movement.

[0029] The working principle of this utility model is as follows: The transfection box 1 and the supporting partition 12 provide support for the transfection container 4 containing cell fluid during gene transfection. When ultrasound-mediated gene transfection is required, the drive motor 11 rotates the first screw 17, which in turn moves the fixed frame 14 and the moving frame 15. The moving frame 15 then moves the moving frame 28 and the transducer 18, allowing the transducer 18 to be positioned below the through-holes 26 at different locations. Simultaneously, the dual-axis motor 24 rotates the second screw 25, which in turn moves the two sets of moving plates 27 to opposite sides. As the moving plates 27 move, they pass the push rod 22. The moving frame 28 and transducer 18 are moved upwards until the top of transducer 18 can be inserted into the through hole 26 and contact the bottom of transfection container 4. At the same time, under the action of ultrasonic generator 13 and transducer 18, transducer 18 can convert the electrical signal generated by ultrasonic generator 13 into high-frequency vibration and ultrasonic waves. Under the action of ultrasonic wave transmission, cavitation effect is generated in the cell fluid inside transfection container 4, thereby creating transient gaps on the cell membrane, increasing cell membrane permeability, and promoting the entry of gene and vector solution into the cell, thus achieving the purpose of ultrasound-mediated gene transfection. Moreover, since the cell fluid inside transfection container 4 can be operated one by one and automatically during gene transfection, the quality of transfection operation is effectively guaranteed, and the work of personnel is greatly facilitated.

[0030] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit the scope of protection of this utility model. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this utility model without departing from the essence and scope of the technical solutions of this utility model.

Claims

1. An ultrasound-mediated gene transfection device, comprising a transfection chamber (1), characterized in that: A support partition (12) is fixedly connected to the lower end of the inner cavity of the transfection box (1). An ultrasonic generator (13) is fixedly installed at the middle of the bottom of the support partition (12). A through hole (26) is opened on the surface of the support partition (12). A transfection container (4) is movably connected to the top of the support partition (12). A drive motor (11) is fixedly installed at the lower end of the right side of the outer surface of the transfection box (1). A first screw (17) is fixedly installed at the output end of the drive motor (11). The left side of the first screw (17) is movably connected to the lower end of the left side of the inner cavity of the transfection box (1) through a bearing. A fixing bracket (14) is threadedly connected to the middle end of the first screw (17). The bottom of the fixed frame (14) is fixedly connected to a movable frame (15). A dual-axis motor (24) is fixedly installed at the middle of the bottom of the inner cavity of the movable frame (15). A second screw (25) is fixedly installed on both sides of the output end of the dual-axis motor (24). The surface of the second screw (25) is movably connected to the lower end of the inner cavity of the movable frame (15) through a bearing. A movable plate (27) is threadedly connected to the middle end of the second screw (25). A push rod (22) is movably connected to the upper end of the movable plate (27) through a pin. A movable frame (28) is movably connected to the upper end of the push rod (22) through a pin. A transducer (18) is fixedly installed on the top of the movable frame (28).

2. The ultrasound-mediated gene transfection device according to claim 1, characterized in that: The top of the dyeing box (1) is connected to a protective cover plate (6) via a hinge. A rubber block (7) is fixedly connected to the front surface of the protective cover plate (6). Hooks (8) are fixedly connected to both ends of the top of the protective cover plate (6). Fasteners (3) are fixedly connected to both ends of the front surface of the dyeing box (1). Rubber bases (2) are fixedly connected to the bottom of the outer surface of the dyeing box (1).

3. The ultrasound-mediated gene transfection device according to claim 1, characterized in that: The top of the support partition (12) is fixedly connected to an annular positioning frame (16), and the lower end of the outer surface of the transfection container (4) is movably connected to the inner cavity of the annular positioning frame (16). The inner cavity of the annular positioning frame (16) is fixedly connected to a rubber ring (23), and the lower end of the outer surface of the transfection container (4) is movably connected to the surface of the rubber ring (23).

4. The ultrasound-mediated gene transfection device according to claim 1, characterized in that: Heating tubes (9) are fixedly installed on the upper ends of both sides of the inner cavity of the transfection box (1), a temperature sensor (10) is fixedly installed at the middle of the top of the support partition (12), and a temperature controller (5) is fixedly installed at the middle of the front surface of the transfection box (1).

5. The ultrasound-mediated gene transfection device according to claim 1, characterized in that: The bottom ends of the movable frame (15) are movably connected to guide crossbars (29), and the two sides of the guide crossbars (29) are fixedly connected to the lower end of the inner cavity of the dyeing box (1).

6. The ultrasound-mediated gene transfection device according to claim 1, characterized in that: Fixing blocks (21) are fixedly connected to the lower ends of both sides of the outer surface of the movable frame (15), and a supporting spring (20) is fixedly connected between the top of the fixing block (21) and the bottom of the movable frame (28).

7. The ultrasound-mediated gene transfection device according to claim 1, characterized in that: Guide slide rods (19) are fixedly connected to both sides of the movable frame (15), and the surface of the movable frame (28) is movably connected to the surface of the guide slide rods (19).

Images