Temperature uniformity adjusting plate in cell differentiation process

Abstract

The utility model relates to the technical field of cell differentiation temperature adjusting plates, and discloses a temperature uniformity adjusting plate in a cell differentiation process, which comprises a heat preservation box, a mounting plate is fixedly connected to the bottom of the inner wall of the heat preservation box, and a temperature control plate is fixedly connected to the inner wall of the mounting plate. Graphene cooling fins I are fixedly connected to the top of the mounting plate, a gel heat insulation plate is fixedly connected to the top of the graphene cooling fins I, graphene cooling fins II are fixedly connected to the periphery of the top of the mounting plate, and gel heat insulation plates are fixedly connected to the outer walls of the multiple graphene cooling fins II; the rear side of the outer wall of the second graphene cooling fin is fixed to the periphery of the inner wall of the heat preservation box. According to the utility model, the bottom of the inner wall of the heat preservation box is fixedly connected with the mounting plate, and meanwhile, the inner wall of the temperature control plate is fixedly connected with the temperature control plate, so that cells in the cell plate placed at the top of the placing plate can be smoothly differentiated.

Description

Technical Field

[0001] This utility model relates to the field of cell differentiation temperature regulation plate technology, and in particular to a temperature uniformity regulation plate during cell differentiation. Background Technology

[0002] Cell differentiation is one of the core research areas in biology. It is of vital importance for understanding the development of organisms, the mechanisms of disease, and the development of regenerative medicine. Temperature is a key environmental factor in the cell differentiation process, which has a significant impact on cell growth, metabolism, and gene expression. As cell differentiation research continues to deepen, researchers have increasingly higher requirements for the precision of experimental conditions, especially the precise control and uniform distribution of temperature. In experiments that induce pluripotent stem cells to differentiate into cardiomyocytes, precise temperature control can improve differentiation efficiency and cell quality, providing a more reliable cell model for the treatment of myocardial diseases. Therefore, the development of a regulating plate that can precisely adjust temperature uniformity has become an urgent need for the development of cell differentiation research.

[0003] Currently available cell differentiation temperature control plates mainly consist of temperature control elements and temperature uniformity protection components. However, due to limitations in heating and cooling methods, traditional culture equipment makes it difficult to guarantee temperature uniformity within the culture space. Temperature gradients may exist within the incubator, with significant temperature differences between areas near heating or cooling elements and areas further away. This temperature inhomogeneity leads to inconsistent cell differentiation at different locations, greatly reducing the reliability of experimental results. The issue of temperature uniformity is particularly prominent when conducting large-scale cell culture for differentiation research, severely impacting the scalability of experiments and the accuracy of data. Furthermore, traditional temperature control equipment is often designed to meet general cell culture needs and is not optimized for the specific temperature requirements of cell differentiation. Its functions are relatively limited, failing to meet the demands for dynamic temperature regulation, precise control, and coordinated control with other experimental conditions in cell differentiation experiments. Some cell differentiation experiments require precise gradient control of temperature changes at different stages, a complex temperature regulation function that traditional equipment struggles to achieve. Utility Model Content

[0004] To overcome the above shortcomings, this utility model provides a temperature uniformity adjustment plate during cell differentiation, aiming to improve the problem that temperature uniformity in the culture space is difficult to guarantee in traditional culture equipment due to limitations in heating and cooling methods.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a temperature uniformity regulating plate for cell differentiation, comprising an incubator, an mounting plate fixedly connected to the bottom of the inner wall of the incubator, a temperature control plate fixedly connected to the inner wall of the mounting plate, a graphene heat sink I fixedly connected to the top of the mounting plate, a gel insulation plate fixedly connected to the top of the graphene heat sink I, graphene heat sink II fixedly connected to all four sides of the top of the mounting plate, gel insulation plates fixedly connected to the outer walls of multiple graphene heat sink II, the rear side of the outer wall of the graphene heat sink II fixed to all four sides of the inner wall of the incubator, a temperature sensor fixedly connected to the outer wall of the rear gel insulation plate, a limiting plate fixedly connected to the inner wall of the incubator near the top, a placement plate slidably connected to the inner side of the outer wall of the limiting plate, and a fixing mechanism provided on all four sides of the inner wall of the incubator for fixing the cell differentiation plate.

[0006] As a further description of the above technical solution:

[0007] The fixing mechanism includes a rotating shaft, with both ends of the multiple rotating shafts rotatably connected to the inner walls of the insulation box. A connecting sleeve is fixedly connected to the outer wall of the rotating shaft, and a spring is fixedly connected to the inner wall of the connecting sleeve. The other end of the spring is fixedly connected to a connecting shaft, and a fixing plate is fixedly connected to the top of the connecting shaft. The outer walls of the placement plate are provided with engaging grooves, and the outer walls of the multiple connecting shafts engage with the outer walls of the engaging grooves.

[0008] As a further description of the above technical solution:

[0009] Protective plates are fixedly connected to the front and rear sides of the bottom of the outer wall of the insulated box, and multiple protective plates are symmetrically arranged on the front and rear sides of the outer wall of the insulated box.

[0010] As a further description of the above technical solution:

[0011] The outer wall of the insulated box is fixedly connected to the top left and right sides, and the outer walls of the multiple handles are fixedly connected to anti-slip sleeves.

[0012] As a further description of the above technical solution:

[0013] Protective plates are fixedly connected to the bottom left and right sides of the outer wall of the insulated box, and multiple protective plates are symmetrically arranged on the left and right sides of the outer wall of the insulated box.

[0014] As a further description of the above technical solution:

[0015] A cell plate is provided on the top of the placement plate, and soft pads are fixedly connected to the four corners of the top of the incubator.

[0016] As a further description of the above technical solution:

[0017] Protective pads are fixedly connected to the top left and right sides of the outer wall of the insulated box, and multiple protective pads are symmetrically arranged on the left side of the outer wall of the insulated box.

[0018] As a further description of the above technical solution:

[0019] Protective strips are fixedly connected to the bottom of the outer wall of the insulated box, and anti-slip pads are fixedly connected to the four corners of the bottom of the insulated box.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, an installation plate is fixedly connected to the bottom of the inner wall of the insulated box, and a temperature control plate is fixedly connected to the inner wall of the temperature control plate to regulate the temperature. A graphene heat sink is fixedly connected to the top of the installation plate to enhance the uniform conduction of heat within the regulating plate. Graphene heat sinks are fixedly connected to all four sides of the top of the installation plate. Gel insulation plates are fixedly connected to the outer walls of both graphene heat sinks and graphene heat sinks to ensure that the cells inside the cell plate placed on top of the placement plate can differentiate smoothly.

[0022] 2. In this utility model, in order to better fix and disassemble the placement plate, a rotating shaft is rotatably connected around the outer wall of the insulated box, and a connecting sleeve is fixedly connected to the outer wall of the rotating shaft. A spring is fixedly connected to the inner wall of the connecting sleeve, and a connecting shaft is fixedly connected to the other end of the spring. By rotating the connecting sleeve, the fixing plate fixed at the top of the connecting shaft can fix the placement plate under the action of the spring. Attached Figure Description

[0023] Figure 1 This is a front perspective view of an insulated box with a temperature uniformity regulating plate during cell differentiation, as proposed in this utility model.

[0024] Figure 2 This is a partial structural diagram of a cell plate for regulating temperature uniformity during cell differentiation, as proposed in this utility model.

[0025] Figure 3 This is a partial structural diagram of a gel insulation plate for regulating temperature uniformity during cell differentiation, as proposed in this utility model.

[0026] Figure 4 This is a partial structural diagram of a graphene heat sink II, a temperature uniformity regulating plate for cell differentiation proposed in this utility model.

[0027] Figure 5 This is a partial structural diagram of a temperature control plate for regulating temperature uniformity during cell differentiation, as proposed in this utility model.

[0028] Figure 6 This is a partial structural diagram of a temperature uniformity regulating leaf spring proposed in this utility model for cell differentiation.

[0029] Legend:

[0030] 1. Insulation box; 2. Fixing mechanism; 201. Rotating shaft; 202. Connecting sleeve; 203. Spring; 204. Connecting shaft; 205. Fixing plate; 206. Engaging groove; 3. Mounting plate; 4. Temperature control plate; 5. Limiting plate; 6. Graphene heat sink one; 7. Graphene heat sink two; 8. Gel insulation plate; 9. Temperature sensor; 10. Placement plate; 11. Handle; 12. Anti-slip sleeve; 13. Protective plate; 14. Protective strip; 15. Soft pad; 16. Cell plate; 17. Protective plate; 18. Anti-slip pad; 19. Protective mat. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Please see the appendix Figure 3 - Appendix Figure 5 This utility model provides an embodiment of a temperature uniformity regulating plate during cell differentiation, comprising an incubator 1, an mounting plate 3 fixedly connected to the bottom of the inner wall of the incubator 1, a temperature control plate 4 fixedly connected to the inner wall of the mounting plate 3, a graphene heat sink 6 fixedly connected to the top of the mounting plate 3, a gel insulation plate 8 fixedly connected to the top of the graphene heat sink 6, graphene heat sinks 7 fixedly connected to all four sides of the top of the mounting plate 3, and gel insulation plates 8 fixedly connected to the outer walls of the multiple graphene heat sinks 7. The rear side of the outer wall of the graphene heat sinks 7 is fixedly... Temperature sensors 9 are fixedly connected to the outer wall of the rear gel insulation plate 8 around the inner wall of the incubator 1. Multiple graphene heat sinks 7 are fixed to the outer wall of the rear side of the inner wall of the incubator 1 by a fixing method. Temperature sensors 9 are fixedly connected to the outer wall of these gel insulation plates 8 for real-time monitoring of temperature changes. A limiting plate 5 is fixedly connected to the inner wall of the incubator 1 near the top. A placement plate 10 is slidably connected to the inner side of the outer wall of the limiting plate 5. Fixing mechanisms 2 are provided around the inner wall of the incubator 1 for fixing the cell differentiation plate.

[0033] Specifically, an installation plate 3 is fixedly connected to the bottom of the inner wall of the incubator 1. A temperature control plate 4 is fixedly connected to the inner wall of the installation plate 3. A graphene heat sink 6 is fixedly connected to the top of the installation plate 3. A gel insulation plate 8 is fixedly connected to the top of the graphene heat sink 6. In addition, graphene heat sinks 7 are fixedly connected to the top of the installation plate 3. The outer walls of the graphene heat sinks 7 are fixedly connected to the gel insulation plate 8. To further improve the insulation effect, a limiting plate 5 is fixedly connected to the inner wall of the incubator 1 near the top. A placement plate 10 is connected to the inner side of the outer wall of the limiting plate 5 through a sliding connection, so that the placement plate 10 can slide under the guidance of the limiting plate 5. Finally, a fixing mechanism 2 is provided on the inner wall of the incubator 1. This fixing mechanism 2 is designed to fix the cell differentiation plate and ensure the stability during the experiment.

[0034] Please see the appendix Figure 4 - Appendix Figure 6 The fixing mechanism 2 includes a rotating shaft 201. Both ends of the multiple rotating shafts 201 are rotatably connected to the inner walls of the insulation box 1. A connecting sleeve 202 is fixedly connected to the outer wall of the rotating shaft 201. A spring 203 is fixedly connected to the inner wall of the connecting sleeve 202. A connecting shaft 204 is fixedly connected to the other end of the spring 203. A fixing plate 205 is fixedly connected to the top of the connecting shaft 204. The top of the connecting shaft 204 is designed to be fixedly connected to the fixing plate 205. This structural design makes the rotating shaft 201, the connecting sleeve 202, the spring 203 and the connecting shaft 204 form a stable assembly. The outer walls of the placement plate 10 are provided with engaging grooves 206. The outer walls of the multiple connecting shafts 204 engage with the outer walls of the engaging grooves 206.

[0035] Specifically, the outer wall of the rotating shaft 201 is designed to be fixedly connected to the connecting sleeve 202. This connection method ensures the stability and reliability between the rotating shaft 201 and the sleeve. The inner wall of the connecting sleeve 202 is further fixedly connected to the spring 203. This design allows the spring 203 to exert its elastic force inside the sleeve. The other end of the spring 203 is fixedly connected to the connecting shaft 204, so that the entire structure can maintain a certain elasticity when subjected to external force. In addition, the outer walls of the placement plate 10 are provided with engaging grooves 206. The outer walls of these engaging grooves 206 engage with the outer walls of multiple connecting shafts 204, thereby ensuring a tight connection between the placement plate 10 and the rotating shaft 201 assembly. This engaging structure not only improves the overall stability of the device, but also facilitates quick disassembly and assembly when needed.

[0036] Please see the appendix Figure 2 - Appendix Figure 4 Protective plates 17 are fixedly connected to the bottom front and rear sides of the outer wall of the insulated box 1. Multiple protective plates 17 are symmetrically arranged on the front and rear sides of the outer wall of the insulated box 1. Handles 11 are fixedly connected to the top left and right sides of the outer wall of the insulated box 1. Anti-slip sleeves 12 are fixedly connected to the outer walls of multiple handles 11 to provide a better grip experience and prevent hand slippage. Protective plates 13 are fixedly connected to the bottom left and right sides of the outer wall of the insulated box 1. Multiple protective plates 13 are symmetrically arranged on the left and right sides of the outer wall of the insulated box 1.

[0037] Specifically, protective plates 17 are fixedly connected to the front and rear sides of the bottom of the outer wall of the insulated box 1. These protective plates 17 are designed to be symmetrically arranged on the front and rear sides of the outer wall of the insulated box 1 to ensure that the insulated box 1 can be fully protected during transportation or movement. In order to further enhance the durability and protective performance of the insulated box 1, protective plates 13 are fixedly connected to the left and right sides of the bottom of its outer wall. These protective plates 13 are also symmetrically arranged on the left and right sides of the outer wall of the insulated box 1 to ensure that the insulated box 1 can maintain its structural integrity in various usage environments.

[0038] Please see the appendix Figure 1 - Appendix Figure 3 A cell plate 16 is provided on the top of the placement plate 10. Soft pads 15 are fixedly connected to the four corners of the top of the incubator 1. Protective pads 19 are fixedly connected to the top left and right sides of the outer wall of the incubator 1. Multiple protective pads 19 are symmetrically arranged on the left side of the outer wall of the incubator 1. The setting of these protective pads 19 further enhances the durability and impact resistance of the incubator 1. The symmetrical arrangement of the protective pads 19 on the left side of the outer wall of the incubator 1 not only ensures the structural balance of the incubator 1, but also makes the incubator 1 more visually coordinated and beautiful. Protective strips 14 are fixedly connected to the bottom four sides of the outer wall of the incubator 1. Anti-slip pads 18 are fixedly connected to the four corners of the bottom of the incubator 1.

[0039] Specifically, a cell plate 16 is carefully designed and installed on the top of the placement plate 10. This design can provide additional protection and support for the items placed in the insulated box 1. Soft pads 15 are fixedly connected to the four corners of the top of the insulated box 1. These pads 15 not only increase the aesthetics of the insulated box 1, but also effectively prevent damage caused by collision or friction when the insulated box 1 is handled or moved. In order to further protect the outer wall of the insulated box 1, protective strips 14 are fixedly connected to the bottom four sides. These protective strips 14 can effectively prevent damage caused by friction with the ground when the insulated box 1 is placed or moved. Finally, anti-slip pads 18 are fixedly connected to the four corners of the bottom of the insulated box 1. These anti-slip pads 18 can not only improve the stability of the insulated box 1 on different ground surfaces and prevent it from sliding, but also absorb impact to a certain extent, thereby protecting the safety of the insulated box 1 and its internal items.

[0040] Working principle: An installation plate 3 is fixedly connected to the bottom of the inner wall of the incubator 1, and a temperature control plate 4 is fixedly connected to the inner wall of the temperature control plate 4 to regulate the temperature. A graphene heat sink 6 is fixedly connected to the top of the installation plate 3 to ensure uniform heat conduction within the regulating plate. Graphene heat sinks 7 are fixedly connected to the top of the installation plate 3 around its perimeter. Gel insulation plates 8 are fixedly connected to the outer walls of both graphene heat sinks 7 and 6. This ensures heat conduction while preventing direct contact between the regulating plate and the temperature plate, which could affect cell differentiation. A placement plate 10 is slidably connected to the inner wall of the incubator 1 near the top-fixed limiting plate 5. A temperature sensor 9 installed on the outer wall of the gel insulation plate 8 monitors the temperature inside, ensuring that the cells inside the cell plate 16 placed on top of the placement plate 10 can differentiate smoothly.

[0041] To better fix and disassemble the placement plate 10, a rotating shaft 201 is rotatably connected around the outer wall of the insulation box 1. At the same time, a connecting sleeve 202 is fixedly connected to the outer wall of the rotating shaft 201. A spring 203 is fixedly connected to the inner wall of the connecting sleeve 202. A connecting shaft 204 is fixedly connected to the other end of the spring 203. By rotating the connecting sleeve 202, the connecting shaft 204 can be engaged in the engaging groove 206 opened on the outer wall of the placement plate 10, so that the fixing plate 205 fixed at the top of the connecting shaft 204 can fix the placement plate 10 under the action of the spring 203.

[0042] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A temperature homogeneity regulation plate for cell differentiation processes, comprising an incubator (1), characterized in that: The inner wall bottom of the incubator (1) is fixedly connected with a mounting plate (3), the inner wall of the mounting plate (3) is fixedly connected with a temperature control plate (4), the top of the mounting plate (3) is fixedly connected with a graphene heat sink I (6), the top of the graphene heat sink I (6) is fixedly connected with a gel heat insulation plate (8), the top of the mounting plate (3) is fixedly connected with a graphene heat sink II (7) around, the outer wall of the plurality of graphene heat sink II (7) is fixedly connected with a gel heat insulation plate (8), the outer wall of the rear side of the graphene heat sink II (7) is fixed to the inner wall of the incubator (1) around, the outer wall of the rear side of the gel heat insulation plate (8) is fixedly connected with a temperature sensor (9), the inner wall of the incubator (1) is fixedly connected with a limiting plate (5) near the top, the outer wall of the limiting plate (5) is slidably connected with a placing plate (10), the inner wall of the incubator (1) is provided with a fixing mechanism (2) around, and the fixing mechanism (2) is used for fixing the cell differentiation plate.

2. A temperature uniformity adjustment plate for use in cell differentiation processes according to claim 1, characterized in that: The fixing mechanism (2) comprises a rotating shaft (201), the inner wall of the incubator (1) is rotatably connected around the two ends of the plurality of rotating shafts (201), the outer wall of the rotating shaft (201) is fixedly connected with a connecting sleeve (202), the inner wall of the connecting sleeve (202) is fixedly connected with a spring (203), the other end of the spring (203) is fixedly connected with a connecting shaft (204), the top of the connecting shaft (204) is fixedly connected with a fixing plate (205), the outer wall of the placing plate (10) is provided with a clamping groove (206) around, and the outer wall of the plurality of connecting shafts (204) is clamped with the outer wall of the clamping groove (206).

3. The temperature uniformity adjustment plate for cell differentiation processes of claim 1, wherein: The outer wall bottom of the incubator (1) is fixedly connected with a protection plate (17) on the front and rear sides, and the plurality of protection plates (17) are symmetrically processed on the front and rear sides of the outer wall of the incubator (1).

4. The temperature uniformity adjustment plate for cell differentiation processes of claim 1, wherein: The outer wall top of the incubator (1) is fixedly connected with a handle (11) on the left and right sides, and the outer wall of the plurality of handles (11) is fixedly connected with an anti-skid sleeve (12).

5. The temperature uniformity adjustment plate for cell differentiation processes of claim 1, wherein: The outer wall bottom of the incubator (1) is fixedly connected with a protection plate (13) on the left and right sides, and the plurality of protection plates (13) are symmetrically processed on the left and right sides of the outer wall of the incubator (1).

6. The temperature uniformity adjustment plate for cell differentiation processes of claim 1, wherein: The outer wall top of the incubator (1) is fixedly connected with a protection pad (19) on the left and right sides, and the plurality of protection pads (19) are symmetrically processed on the left side of the outer wall of the incubator (1).

7. The temperature uniformity adjustment plate for cell differentiation processes of claim 1, wherein: The outer wall bottom of the incubator (1) is fixedly connected with a protection strip (14) around, and the bottom of the incubator (1) is fixedly connected with an anti-skid pad (18) at the four corners.

8. The temperature uniformity adjustment plate for cell differentiation processes of claim 1, wherein: The top of the placing plate (10) is provided with a cell plate (16), and the top of the incubator (1) is fixedly connected with a soft pad (15) at the four corners. The outer wall top of the incubator (1) is fixedly connected with a protection pad (19) on the left and right sides, and the plurality of protection pads (19) are symmetrically processed on the left side of the outer wall of the incubator (1). The outer wall bottom of the incubator (1) is fixedly connected with a protection strip (14) around, and the bottom of the incubator (1) is fixedly connected with an anti-skid pad (18) at the four corners.

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