Glass slide with high adhesion to suspension cells

Abstract

The utility model relates to the technical field of biomedical experiment equipment. The utility model discloses a high-adhesion suspension cell glass slide, which comprises a body and a cover glass, the body is provided with an inward concave sample loading area, one side of the body close to the edge is provided with an outward convex body projection, at least one fixing groove and a plurality of drainage grooves are arranged on the body projection in a penetrating manner, and the cover glass is arranged on the fixing groove. A cover glass protruding part corresponding to the fixing groove is arranged on one side of the cover glass, and the cover glass is fixedly attached to the body through the body protruding part. The body protruding part is matched with the cover glass protruding part, so that accurate alignment and rapid installation are provided, and the operation process is simplified; due to the design of a frosted cover glass protruding part and an anti-skid supporting point, the operation safety and the placement stability of the glass slide are improved; and liquid management is optimized through the reasonable liquid supplementing tank and plane layout, and the pollution risk is reduced.

Description

Technical Field

[0001] This utility model relates to the field of biomedical experimental equipment technology, specifically to a glass slide with highly adhesive suspended cells. Background Technology

[0002] In biomedical research, glass slides are essential tools for holding and observing samples such as cells and tissue sections. For studies of suspended cells, ensuring stable cell adhesion to the slide is crucial. Traditional slide designs have several shortcomings, especially when handling suspended cells, often facing the following problems: Traditional slides have smooth surfaces that fail to provide sufficient adhesive force to secure suspended cells, leading to cell detachment or drift, affecting subsequent microscopic observation and analysis. Even with coatings to improve adhesion, the lack of a well-designed structure cannot guarantee long-term stable cell adhesion. When covering with coverslips, the absence of a clear stress point requires operators to maintain high concentration and careful control to prevent sample misalignment or damage. Even after successful covering, slight contact can cause the coverslip to shift, distorting the sample image and affecting experimental results. When placing slides containing detection substances into the detection device, spring clips or other clamps are typically used to secure the slides. However, due to the extremely smooth surface of the slide and the small contact area between the spring clip and the slide, this fixing method has poor stability. The spring clip may also slip or shift, further reducing the reliability and repeatability of experimental data. Existing slide designs often neglect convenience and safety in practical operation. For example, the lack of an effective drainage mechanism may lead to liquid residue, increasing the risk of contamination; the lack of a design to prevent moisture adsorption affects the stability of the slide placement. The liquid replenishment process is complex, and the unreasonable layout of the replenishment section and plane increases the difficulty of operation and the potential error rate. Utility Model Content

[0003] To address the shortcomings of existing technologies, this utility model discloses a high-adhesion suspended cell slide and its matching coverslip fixation structure that can effectively solve the above problems.

[0004] This utility model discloses a glass slide with high adhesion of suspended cells, which includes a body and a coverslip. The body is provided with an inwardly recessed sample carrying area, and an outwardly protruding body protrusion is provided on one side of the body near the edge. At least one fixing groove and several drainage grooves are passed through the body protrusion. One side of the coverslip is provided with a coverslip protrusion corresponding to the fixing groove. The coverslip is fixedly attached to the body through the body protrusion.

[0005] Furthermore, a liquid replenishment groove is passed through the protruding part of the main body, and a liquid replenishment part is provided on one side of the main body that protrudes outward corresponding to the liquid replenishment groove. The liquid replenishment part extends into the sample loading area. The plane of the main body near the protruding part of the main body is taken as the horizontal plane, the plane of the liquid replenishment part is taken as the liquid replenishment part plane, and the plane of the sample loading area is taken as the sample loading area plane. The distance between the sample loading area plane and the horizontal plane is greater than the distance between the liquid replenishment part plane and the horizontal plane.

[0006] Furthermore, the thickness of the body is 2-2.4 cm, the height of the protrusion of the body is 0.5-0.8 cm, the thickness of the liquid replenishment part is 0.5-0.8 cm, and the distance between the sample loading area and the protrusion of the body is 0.7-1.2 cm.

[0007] Furthermore, the corners around the sample loading area are rounded.

[0008] Furthermore, at least four outwardly protruding parts are provided on the side of the main body away from the protruding part of the main body. The protruding parts are provided on the side close to the edge of the main body. The end of the protruding part away from the main body is rough and the distance between the end of the protruding part away from the main body and the main body is 0.5-1cm.

[0009] Furthermore, the protruding part of the cover glass is frosted.

[0010] Furthermore, the side of the slide near the protruding part of the body is coated with a coating, such as an aminosilane coating, a poly-L-lysine coating, a collagen coating, or a fibronectin coating.

[0011] The beneficial effects of this utility model are:

[0012] The interlocking of the main body protrusion and the coverslip protrusion provides precise alignment and rapid installation, simplifying the operation process. The frosted coverslip protrusion and anti-slip support points enhance operational safety and slide placement stability. The rationally designed replenishment tank and planar layout optimize liquid management and reduce the risk of contamination. In summary, this novel slide design not only addresses many shortcomings of traditional slides but also provides users with an efficient, reliable, and easy-to-manage experimental platform, particularly suitable for research scenarios requiring strict control of cell adhesion conditions. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of a glass slide in one embodiment of this application.

[0014] Figure 2 This is a schematic diagram of another structure of the glass slide in the embodiments of this application.

[0015] In the figure: slide 100, body 11, body protrusion 111, liquid replenishment part 112, protrusion 113, coverslip 12, coverslip protrusion 121. Detailed Implementation

[0016] To enable those skilled in the art to better understand the present invention, the technical solutions in the specific embodiments of the present invention will be clearly and completely described below.

[0017] This utility model discloses a glass slide 100 with high adhesion of suspended cells, such as... Figure 1 As shown, it includes a body 11 and a cover glass 12, as... Figure 2As shown, the body 11 has an inwardly recessed sample-carrying area, and a protruding body protrusion 111 is provided on one side of the body 11 near the edge. At least one fixing groove and several drainage grooves are passed through the body protrusion 111. A coverslip protrusion 121 corresponding to the fixing groove is provided on one side of the coverslip 12. The coverslip 12 is fixedly attached to the body 11 via the body protrusion 111. The inwardly recessed sample-carrying area provides a relatively closed and stable microenvironment for the cells, helping them to better adhere to the surface of the slide 100 and reducing cell loss due to liquid flow or shaking. The design of the sample-carrying area ensures that the cells are evenly distributed in the sample solution, avoiding problems of excessively high or low local concentrations and improving the representativeness of the sample. The fixing groove on the body protrusion 111 cooperates with the coverslip protrusion 121, allowing the coverslip 12 to be quickly and firmly attached to the body, simplifying the operation steps and reducing the time and difficulty of manual adjustment. The presence of several drainage channels allows excess liquid to drain promptly during sample loading, preventing spillage and contamination of other areas, while also helping to maintain appropriate humidity within the sample loading area. The coverslip 12 fits snugly against the body 11, forming a sealed space that protects the sample from external dust, contaminants, or airflow, ensuring sample purity and integrity. The engagement of the fixing groove with the coverslip protrusion 121 ensures that the coverslip 12 automatically aligns to the correct position during installation, reducing the need for manual adjustments and improving assembly accuracy. This is particularly important for applications requiring precise positioning. By providing clearly defined stress points, this design effectively prevents accidental disturbance to the sample during coverslip coverage, ensuring stable cell attachment to the slide 100 and avoiding cell detachment or drift due to improper operation. Precise positioning prevents the coverslip 12 from causing physical damage to the sample during coverage, such as crushing or scratching cells, thereby protecting sample integrity and the accuracy of experimental results. The tight fit between the fixing groove and the coverslip protrusion 121 provides a stable connection without increasing mechanical stress, avoiding the loosening or detachment problems that may occur with traditional fixing methods, and enhancing the overall structural stability. In summary, the limiting effect provided by the fixing groove on the body protrusion 111 and the coverslip protrusion 121 significantly improves the functionality and practicality of the highly adhesive suspended cell slide 100. This design not only simplifies the operation process and improves the stability of cell adhesion and the consistency of experimental results, but also enhances the safety and reliability of the equipment, providing users with an efficient, reliable, and easy-to-manage experimental platform, particularly suitable for the separation and analysis of suspended cells in molecular biology, cell biology, and clinical diagnostics.

[0018] In one embodiment, a replenishment tank extends through the protrusion 111 of the main body. A replenishment part 112, corresponding to the replenishment tank, is provided on one side of the main body 11, protruding outwards. The replenishment part 112 extends into the sample loading area. Taking the plane of the main body 11 closest to the protrusion 111 as the horizontal plane, the plane of the replenishment part 112 is called the replenishment part 112 plane, and the plane of the sample loading area is on the sample loading area plane. The distance between the sample loading area plane and the horizontal plane is greater than the distance between the replenishment part 112 plane and the horizontal plane. The replenishment part 112 directly connects to and extends into the sample loading area, making the replenishment process more direct and convenient, reducing liquid transfer steps, and lowering the risk of contamination caused by multiple transfers. Users can easily add or adjust the sample liquid volume through the replenishment tank, ensuring accurate volume and location for each replenishment. Because the sample loading area plane is higher than the replenishment part 112 plane, even if slight overflow occurs during replenishment, the liquid will first remain in the replenishment part 112 area and will not flow directly into the sample loading area, avoiding the risk of sample dilution or contamination. The design of the replenishment section 112 reduces the potential impact on already attached cells when adding liquid directly to the sample loading area, protecting cell integrity and distribution, and ensuring the accuracy of subsequent microscopic observation and analysis. The higher plane of the sample loading area helps maintain a relatively closed and stable microenvironment, reducing the influence of external factors such as airflow and temperature changes on the sample, and enhancing sample stability throughout the experiment. The height difference promotes the natural flow of liquid from the replenishment section 112 to the sample loading area, forming a reasonable flow path, ensuring uniform liquid distribution, and avoiding localized over- or under-replenishment.

[0019] In one embodiment, the thickness of the body 11 is 2-2.4 cm, the height of the protrusion 111 is 0.5-0.8 cm, the thickness of the liquid replenishment part 112 is 0.5-0.8 cm, and the distance between the sample loading area and the protrusion 111 is 0.7-1.2 cm. The relatively thick body 11 (2-2.4 cm) provides sufficient mechanical strength to ensure that the slide 100 remains stable under various operating conditions, is not easily deformed or damaged, and extends the service life of the equipment.

[0020] As one implementation method, the corners around the sample loading area are rounded. Rounding eliminates cleaning dead spots that may exist in traditional right-angle designs, allowing cleaning tools such as cotton swabs and brushes to more easily reach and thoroughly clean every corner, ensuring the surface of the slide 100 is completely clean. The smooth edges reduce the possibility of dirt, cell debris, or chemical residues, lowering the risk of cross-contamination, which is especially important in frequently used laboratory environments. Rounding reduces friction between cleaning tools and the edges of the slide 100, reducing the risk of wear and tear due to prolonged use, thereby extending the lifespan of the equipment. The smooth edges reduce the risk of scratches to operators' hands or other contact points, improving the safety of experimental operations.

[0021] In one implementation, at least four outwardly protruding portions 113 are provided on the side of the body 11 away from the protruding portion 111. The protruding portions 113 are located near the edge of the body 11, with the end of each protruding portion 113 away from the body 11 having a rough finish. The distance between this end and the body 11 is 0.5-1 cm. The presence of the protruding portions 113 maintains a certain gap of 0.5-1 cm between the slide 100 and the test platform, reducing the direct contact area and significantly reducing adhesion caused by residual moisture, making the slide 100 easier to remove. The gap promotes airflow at the bottom of the slide 100, facilitating rapid evaporation of residual moisture and further reducing the possibility of adhesion. Even in humid environments, the design of the protruding portions 113 ensures that the slide 100 does not adhere tightly to the test platform, allowing users to easily pick up or place the slide 100, reducing operational difficulty and time costs.

[0022] As one implementation, the cover glass protrusion 121 is frosted. The frosted surface increases the friction between the fingers and the cover glass 12, making it easier for the user to grip and move the cover glass 12 and reducing the risk of slipping due to the smooth surface.

[0023] In one embodiment, the side of the slide 100 near the protrusion 111 of the body is coated with a coating, such as an aminosilane coating, a poly-L-lysine coating, a collagen coating, or a fibronectin coating. These coating materials, such as aminosilane, poly-L-lysine, collagen, and fibronectin, have high biocompatibility and hydrophilicity, which can significantly improve the adhesion of suspended cells to the slide 100 and reduce the risk of cell detachment or drift.

[0024] It should be understood that those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims.

Claims

1. A glass slide with highly adhesive suspended cells, characterized in that, include: The main body has an inwardly recessed sample carrying area and an outwardly protruding part on one side of the main body near the edge. At least one fixing groove and several drainage grooves are passed through the protruding part of the main body. A cover glass slide has a cover glass slide protrusion on one side that corresponds to a fixing groove. The cover glass slide is fixedly attached to the body through the protrusion.

2. The glass slide for highly adhesive suspended cells according to claim 1, characterized in that: A liquid replenishment groove runs through the protruding part of the main body. A liquid replenishment part is provided on one side of the main body, which protrudes outward corresponding to the liquid replenishment groove. The liquid replenishment part extends into the sample loading area. The plane of the main body near the protruding part is the horizontal plane, the plane of the liquid replenishment part is the liquid replenishment part plane, and the plane of the sample loading area is the sample loading area plane. The distance between the sample loading area plane and the horizontal plane is greater than the distance between the liquid replenishment part plane and the horizontal plane.

3. The glass slide for highly adhesive suspended cells according to claim 2, characterized in that: The thickness of the main body is 2-2.4cm, the height of the protrusion of the main body is 0.5-0.8cm, the thickness of the liquid replenishment part is 0.5-0.8cm, and the distance between the sample loading area and the protrusion of the main body is 0.7-1.2cm.

4. The glass slide for highly adhesive suspended cells according to claim 1, characterized in that: The edges and corners around the sample loading area are rounded.

5. The glass slide for highly adhesive suspended cells according to claim 1, characterized in that: At least four outwardly protruding parts are provided on the side of the main body away from the protruding part of the main body. The protruding parts are located on the side close to the edge of the main body. The end of the protruding part away from the main body is rough. The distance between the end of the protruding part away from the main body and the main body is 0.5-1cm.

6. The glass slide for highly adhesive suspended cells according to claim 1, characterized in that: The protruding part of the cover glass is frosted.

7. The glass slide for highly adhesive suspended cells according to claim 1, characterized in that: The side of the slide near the protrusion of the body is coated with a coating, such as an aminosilane coating, a poly-L-lysine coating, a collagen coating, or a fibronectin coating.

Images