Bioreactor

Abstract

The utility model discloses a bioreactor which comprises a stirring device, an aeration device and a bag body, the stirring device comprises an impeller, the impeller is arranged in the bag body, and the aeration device comprises a base; an air passage; a bearing part is arranged in the aeration disc and the air passage, and the bearing part is provided with a first sealing surface facing the impeller; a second sealing surface surrounding the peripheral side of the aeration disc is also arranged in the air passage; the aeration device further comprises a peripheral sealing part which is respectively propped against the peripheral surface of the aeration disc and the second sealing surface; the inner sealing part is respectively propped against the outer surface of the aeration disc and the first sealing surface. According to the utility model, through the inner sealing part and the peripheral sealing part, gas in the gas passage can be prevented from escaping from the gas passage through the gap between the aeration disc and the gas passage to form escape bubbles with larger volume, so that the gas in the gas passage mainly escapes from the aeration disc to form diffusion bubbles with smaller volume and higher flow speed; therefore, the diffused bubbles rapidly flow to the impeller and are scattered by the impeller to be transmitted to all positions of the bag body, and the stability of the KLa value is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of biological pharmacy, concretely relates to a kind of bioreactor. BACKGROUND

[0002] In biological pharmacy process, in order to realize commercialization mass production of enzyme, antibody or antibiotic biological product, usually, animal, plant or microbial cell is placed in bioreactor to carry out sterile culture, during which, through the way such as control mixing system, gas supply and inductive probe monitoring, culture process is controlled, and the desired biological product is obtained.In recent years, with the quality requirement of biological product, operation convenience and cost control are considered, and bioreactor is widely used in biological cell culture market.

[0003] Currently used bioreactor is mostly disposable bag, bag is equipped with stirring device and aeration device, aeration device provides gas into bag, stirring device scatters gas and transmits to each place of bag, so as to meet the culture demand of cell.KLa value is an important parameter in bioreactor process, and maintaining appropriate KLa value is one of important conditions to ensure cell growth and product yield and quality stability.Traditional aeration device is usually installed aeration disc on base provided with gas chamber, aeration disc covers gas chamber, so that gas in gas chamber escapes into bag as diffusion bubble through aeration disc, due to the error of processing technology, gap is easily generated between aeration disc and base, gas in gas chamber escapes as escape bubble through gap, compared with normal diffusion bubble escaping from gas hole of aeration disc, escape bubble is larger in size and smaller in diffusion speed, which causes escape bubble to be easily diffused away from stirring device center under the push of stirring device, so that large size escape bubble cannot be fully scattered in time by stirring device, thereby affecting the stability of KLa value. UTILITARIAN CONTENT

[0004] The technical problem to be solved by the utility model is to provide a kind of bioreactor, to solve the problem of large size escape bubble generated between aeration disc and base of existing bioreactor due to poor sealing.

[0005] To solve the above technical problems, the utility model discloses the following technical scheme: a bioreactor, including stirring device, aeration device and the bag body by the flexible material, stirring device includes the impeller, and the impeller is located in the bag body, and aeration device includes: base, is located in the bag body, including the base inner surface towards the impeller and the base outer surface away from the impeller, gas channel, is located on the base, has the gas inlet end and the gas outlet end towards the impeller setting, aeration disc, at least partial embedding in the gas channel, it has the aeration disc inner surface towards the impeller, the aeration disc outer surface away from the impeller and the aeration disc peripheral surface and be located in the aeration disc peripheral edge, the gas channel is equipped with the receiving part in, and the receiving part has the first sealing surface towards the gas outlet end, the gas channel is equipped with the second sealing surface in also around the aeration disc peripheral side, aeration device still includes, peripheral sealing part, around the aeration disc peripheral side, and the sealing is formed between the two by respectively with the aeration disc peripheral surface and the second sealing surface abuts, inner sealing part, the sealing is formed between the two by respectively with the aeration disc outer surface and the first sealing surface abuts, and inner sealing part and peripheral sealing part sealing connection, and along the radial of aeration disc from peripheral sealing part to the central position of it extends.

[0006] The technical scheme has the following technical effects:

[0007] The utility model discloses a support part is set up in the air channel, and the support part can support the aeration disc, and the contact area between the air channel and the aeration disc is increased, and the gap length between the aeration disc and the air channel is increased, and the peripheral sealing part is arranged in the gap between the peripheral surface of the aeration disc and the second sealing surface on the inner side wall of the air channel, and the gap between the peripheral surface of the aeration disc and the second sealing surface is filled, and the peripheral surface of the aeration disc and the second sealing surface are sealed, and the inner sealing part is arranged in the gap between the outer surface of the aeration disc and the first sealing surface on the support part, and the gap between the outer surface of the aeration disc and the first sealing surface is filled, and the outer surface of the aeration disc and the first sealing surface are sealed, and the peripheral sealing part seals the gap between the peripheral surface of the aeration disc and the air channel, and the inner sealing part seals the gap between the outer surface of the aeration disc and the air channel, and the aeration disc embedded in the air channel is double sealed, the inner sealing part seals the aeration disc and the air channel for the first time, and the peripheral sealing part seals the aeration disc and the air channel for the second time, and the sealing effect between the aeration disc and the air channel is improved, and the peripheral sealing part and the inner sealing part are connected, and the gap between the air channel and the aeration disc is continuously filled, and the sealing path of the peripheral sealing part and the inner sealing part is continuous, and the sealing effect is further improved.

[0008] In the biological reactor, the radial extension length of the inner sealing part is greater than the maximum radial extension length of the support part. By setting the radial extension length of the inner sealing part along the aeration disc to be greater than the radial extension length of the support part along the aeration disc, the inner sealing part and the support part are completely matched, the sealing between the inner sealing part and the support part is maximized, the sealing area between the inner sealing part and the aeration disc is increased, and the sealing effect between the inner sealing part and the aeration disc is enhanced, so that the gas in the air channel is prevented from escaping from the gap between the inner sealing part and the support part or the gap between the inner sealing part and the aeration disc.

[0009] In the above-mentioned bioreactor, the inner sealing part extends along the radial direction of the aeration disc by a length L1, and the receiving part extends along the radial direction of the aeration disc by a length L2, and 0.8≤L1 / L2≤1.5 is satisfied. If the ratio of L1 and L2 is too large, it means that L1 is too large, and the inner sealing part will block too much of the aeration disc, and the part of the aeration disc blocked by the inner sealing part cannot be aerated, which reduces the effective aeration area of the aeration disc, causes the gas in the gas channel to be discharged slowly, increases the gas pressure in the gas channel, and high gas pressure will push the aeration disc out of the gas channel, causing the aeration disc to loosen and not be able to be stably sealed with the gas channel. If the ratio of L1 and L2 is too small, it means that the part of the inner sealing part extending inwardly beyond the receiving part is shorter, i.e., the inner sealing part extends less along the sealing path, resulting in poor sealing effect. By setting L1 / L2 in the range of 0.8≤L1 / L2≤1.5, the sealing path of the inner sealing part to the aeration disc is lengthened, thereby extending the bubble escape time, prompting most of the bubbles to further escape through the aeration disc, thereby increasing the number of diffused bubbles, which is conducive to the diffused bubbles being fully dispersed, and ultimately conducive to ensuring the stability of the KLa value.

[0010] In the above-mentioned bioreactor, the gas channel includes, in sequence along the gas flow direction, a first gas guiding section away from the impeller and a second gas guiding section close to the impeller, and the inner diameter of the second gas guiding section is greater than that of the first gas guiding section to form a receiving part at the connection between the second gas guiding section and the first gas guiding section. By setting the inner diameter of the second gas guiding section to be greater than that of the first gas guiding section, a stepped receiving part is formed at the connection between the second gas guiding section and the first gas guiding section, and the receiving part is arranged at the part of the gas channel close to the impeller, facilitating the installation and removal of the aeration disc. At the same time, the stepped receiving part has high strength and is not prone to breaking, and has good support effect on the aeration disc. Alternatively, an annular protruding rib protruding from the inner side wall of the gas channel is arranged on the gas channel to form the receiving part. The receiving part is formed by the annular protruding rib protruding from the inner side wall of the gas channel, which is convenient for production and processing and has good gas guiding effect.

[0011] In the above-mentioned bioreactor, a first flow disturbing part is arranged at a position close to the outer edge of the inner surface of the aeration disc. The first flow disturbing part is arranged at a position close to the outer edge of the inner surface of the aeration disc to promote the flow disturbing effect on the fluid at this position, increase the turbulent flow of the liquid at this position, and thereby enable the diffused bubbles generated by the aeration disc to be dispersed in time at this position and spread in the bag body, which is conducive to improving the stability of the KLa value.

[0012] In the above-mentioned bioreactor, the end surface of the first turbulence portion facing the impeller is a turbulence surface, and the turbulence surface protrudes from the inner surface of the aeration disc. The first turbulence portion is provided to protrude from the inner surface of the aeration disc, and the first turbulence portion is a continuous annular structure. The first turbulence portion is continuously provided on the diffusion path of the diffusion bubbles and protrudes from the inner surface of the aeration disc, so that a large number of diffusion bubbles can collide with the first turbulence portion and break, and the dispersion effect is better.

[0013] In the above-mentioned bioreactor, the first turbulence portion at least meets one of the following four conditions: (1) the distance between the turbulence surface and the inner surface of the base in the axial direction of the aeration disc is D1, the distance between the turbulence surface and the inner surface of the aeration disc in the axial direction of the aeration disc is D2, and 1.5≤D1 / D2≤5 is met; if the ratio of D1 and D2 is too small, the turbulence effect of the first turbulence portion on the fluid is poor; if the ratio of D1 and D2 is too large, the height of the first turbulence portion is too high, which easily causes interference with the rotation of the impeller; by controlling the ratio of D1 and D2 within the range of 1.5≤D1 / D2≤5, the rotation of the impeller is avoided from being interfered by the first turbulence portion, and the turbulence effect is promoted at the same time. (2) the distance between the turbulence surface and the inner surface of the aeration disc in the axial direction of the aeration disc is D2, and the effective gas outlet area of the inner surface of the aeration disc is S; 0.05≤D2 / S≤1 is met; the effective gas outlet area of the inner surface of the aeration disc refers to the area of the region exposed inside the bag body and available for gas passing through without being blocked by any shielding object; if the ratio of D2 and S is too large, the distance between the turbulence surface and the inner surface of the aeration disc in the axial direction of the aeration disc is too large, and in the actual use or processing process, the base may be bent towards the direction of the impeller, so that the first turbulence portion is likely to interfere with the rotation of the impeller; if the ratio of D2 and S is too small, the distance between the turbulence surface and the inner surface of the aeration disc in the axial direction of the aeration disc is too small, so that the turbulence of the first turbulence portion on the inner surface of the aeration disc is insufficient, resulting in small turbulence near the inner surface of the aeration disc; by controlling the ratio of D2 and S within the range of 0.05≤D2 / S≤1, the rotation of the impeller is avoided from being interfered, and the amount of turbulence of the inner surface of the aeration disc is increased at the same time, so that the diffusion bubbles can be dispersed and quickly diffused on the inner surface of the aeration disc. (3) the thickness of the inner sealing portion in the axial direction of the aeration disc is H1, the thickness of the first turbulence portion in the axial direction of the aeration disc is H2, and 0.3≤H1 / H2≤3 is met; if the ratio of H1 and H2 is too large, the axial height of the first turbulence portion is too small, resulting in poor turbulence effect of the first turbulence portion; if the ratio of H1 and H2 is too small, the axial height of the inner sealing portion is too small, and the deformation amount of the thin inner sealing portion is small; if the aeration disc is loose and displaced away from the receiving portion, the inner sealing portion cannot fill the excess gap between the aeration disc and the receiving portion by a certain amount of deformation, so that the sealing effect between the aeration disc and the receiving portion is affected, resulting in poor sealing effect between the aeration disc and the receiving portion. By controlling the ratio of H1 and H2 within the range of 0.3≤H1 / H2≤3, the sealing between the aeration disc and the receiving portion is ensured, and the turbulence effect of the first turbulence portion is enhanced at the same time.(4) The width of the inner sealing part along the radial direction of the aerator plate is L1, and the width of the first turbulence part along the radial direction of the aerator plate is L3, and 0.5≤L1 / L3≤3 is satisfied; if the ratio of L1 and L3 is too large or too small, the inner sealing part will excessively shield the aerator plate, thereby reducing the effective air inlet area of the outer surface of the aerator plate, and the effective air inlet area of the outer surface of the aerator plate refers to the area of the outer surface of the aerator plate that is not shielded by any shielding object and can be passed by gas; the reduction of the effective air inlet area of the outer surface of the aerator plate will cause the gas in the air channel to be unable to flow out quickly through the aerator plate, thereby increasing the air pressure in the air channel; by controlling the ratio of L1 and L3 within the range of 0.5≤L1 / L3≤3, the airflow can be ensured to pass through the aerator plate quickly, and the air pressure in the air channel can be prevented from rising.

[0014] In the above-mentioned bioreactor, a second turbulence part is arranged on the inner surface of the base and surrounds the peripheral sealing part. The second turbulence part is arranged around the peripheral sealing part to promote the turbulence of the fluid at this position and increase the turbulent flow of the liquid at this position, so as to assist the impeller in timely breaking up the diffusion bubbles generated by the aerator plate and improve the stability of the KLa value.

[0015] In the above-mentioned bioreactor, the second turbulence part is arranged to protrude from the inner surface of the base, the spacing between the turbulence surface and the end surface of the second turbulence part facing the impeller in the axial direction of the aerator plate is D3, and the spacing between the turbulence surface and the inner surface of the base in the axial direction of the aerator plate is D1, and 0.1≤D3 / D1≤2 is satisfied; the second turbulence part protruding from the inner surface of the base not only enhances the bubble breaking effect of the second turbulence part, but also increases the strength of the base at this position, which is beneficial to avoiding the interference of the rotation of the impeller when the base bends towards the impeller during actual use or processing. Because when the spacing D3 between the end surface of the second turbulence part facing the impeller and the turbulence surface in the axial direction of the aerator plate increases, the spacing D1 between the turbulence surface and the inner surface of the base in the axial direction of the aerator plate also increases, and the two are positively correlated, therefore, by setting the ratio of D3 and D1 within the range of 0.1≤D3 / D1≤2, the turbulence and the interference effect of the height of the first turbulence part and the second turbulence part on the impeller can be considered. Alternatively, the second turbulence part is arranged to be concave in the inner surface of the base, the spacing between the end surface of the second turbulence part facing the impeller and the turbulence surface in the radial direction of the aerator plate is D4, and 5≤D4 / D1≤10 is satisfied. By setting the ratio of D4 and D1 within the range of 5≤D4 / D1≤10, the turbulence and the interference of the height of the first turbulence part and the second turbulence part on the impeller can be considered.

[0016] In the above-mentioned bioreactor, the first turbulence part extends along the radial direction of the aeration disc and abuts against the inner surface of the aeration disc to seal the space therebetween, and the outer periphery of the first turbulence part is connected to the peripheral sealing part to form a sealing groove for embedding the periphery of the aeration disc together with the first turbulence part and the peripheral sealing part. The periphery of the aeration disc is embedded in the sealing groove, so that the first turbulence part not only increases the turbulent flow on the aeration disc, but also extends the sealing area between the inner sealing part, the peripheral sealing part and the aeration disc as a sealing structure. The first turbulence part, the peripheral sealing part and the inner sealing part collectively wrap around the periphery of the aeration disc, and form a continuous and uninterrupted sealing path around the periphery of the aeration disc, which is conducive to improving the sealing effect and preventing the peripheral sealing part and the inner sealing part from being detached from the aeration disc, thereby enhancing the sealing effect of the periphery of the aeration disc and preventing the gas in the gas channel from escaping between the aeration disc and the gas channel.

[0017] In the above-mentioned bioreactor, the base is provided with a turbulence groove, and the opening of the turbulence groove faces the impeller; or the bag body is provided with a base plate, the impeller is rotationally connected to the base plate, the base plate is provided with a turbulence groove, and the opening of the turbulence groove faces the impeller; or the bag body is provided with a base plate, the impeller is rotationally connected to the base plate, the base and the base plate are an integral structure, a turbulence groove is arranged at the connection between the base and the base plate, and the opening of the turbulence groove faces the impeller; or the bag body is provided with a base plate, the impeller is rotationally connected to the base plate, the base and the base plate are a split structure, the base surrounds the base plate, the inner diameter of the base is greater than the outer diameter of the base plate, so as to form a turbulence gap between the base plate and the base, a flexible member is connected between the base plate and the base, and the flexible member seals one end of the turbulence gap away from the impeller, so as to form a turbulence groove with an opening facing the impeller. The inner bottom surface of the turbulence groove and the inner surface of the base have a height difference, so that the fluid flowing through the turbulence groove forms a turbulent flow near the turbulence groove, increases the turbulent flow near the turbulence groove, and enhances the bubble breaking effect near the turbulence groove.

[0018] In the above-mentioned bioreactor, the impeller includes an impeller shaft and a plurality of blades, the plurality of blades are distributed along the circumferential direction of the impeller shaft, each blade includes a mounting end connected to the impeller shaft and a free end away from the impeller shaft, and the side of the blade close to the aeration device is a stirring edge connected between the mounting end and the free end, and a part of the stirring edge is provided with a turbulence part in a zigzag shape. The turbulence part is used to enhance the turbulent flow effect of the stirring edge, so that the diffusion bubbles generated by the aeration disc can be broken and diffused in time by the impeller, and the stability of the KLa value is ensured.

[0019] In the aforementioned bioreactor, the projection plane is a plane perpendicular to the impeller axis. The projections of the base and the turbulence channel on this plane all fall within the projection of the rotating region of the turbulent section. The diffused bubbles generated by the aeration disc, after leaving the aeration disc, diffuse towards the impeller along its axial direction. By concentrating the turbulent section at the position opposite the aeration disc on the stirring edge, the turbulent section of the impeller can generate a large amount of turbulence along the path of the diffused bubbles as the impeller rotates, thus dispersing the diffused bubbles and assisting in their rapid diffusion.

[0020] The features and advantages of this utility model will be disclosed in detail in the following specific embodiments and accompanying drawings. Attached Figure Description

[0021] The present invention will be further described below with reference to the accompanying drawings and specific embodiments:

[0022] Figure 1 This is a schematic diagram of the overall structure of the stirring device and aeration device in Example 1;

[0023] Figure 2 This is a cross-sectional view of the stirring device and the aeration device in Example 1;

[0024] Figure 3 This is a schematic diagram of the aeration device in Example 1;

[0025] Figure 4 This is a partial cross-sectional view of the aeration device in Example 1;

[0026] Figure 5 for Figure 4 Enlarged view of part A;

[0027] Figure 6 for Figure 4 Enlarged view of part B;

[0028] Figure 7 This is a partial cross-sectional view of the aeration device in Example 2.

[0029] Figure label:

[0030] 100. Impeller; 110. Impeller shaft; 120. Blade; 121. Mounting end; 122. Free end; 123. Agitator edge;

[0031] 200. Substrate;

[0032] 310. Fluid flow channel; 320. Flexible component;

[0033] 400, Base; 410, Inner surface of base; 420, Outer surface of base; 430, Second spoiler;

[0034] 500, air passage; 510, first air passage section; 511, air inlet end; 520, second air passage section; 521, air outlet end; 530, second sealing surface; 540, receiving portion; 541, first sealing surface;

[0035] 600, aerator plate; 610, inner surface of aerator plate; 620, outer surface of aerator plate; 630, peripheral surface of aerator plate;

[0036] 700, peripheral sealing portion;

[0037] 800, inner sealing portion;

[0038] 900, first turbulence portion; 910, turbulence surface. DETAILED DESCRIPTION

[0039] The utility model provides a kind of bioreactor, including stirring device, aeration device and the bag body made of flexible material, stirring device includes impeller, and impeller is located in bag body, aeration device includes: pedestal, positioned in bag body, including the inner surface of pedestal towards impeller and the outer surface of pedestal away from impeller;Air passage, located on pedestal, with air inlet end and the air outlet end towards impeller arrangement;Aerator, at least part is embedded in air passage, it has the inner surface of aerator towards impeller, the outer surface of aerator away from impeller and the aerator peripheral edge surface being located in aerator peripheral edge;Air passage is equipped with receiving part, receiving part has the first sealing surface towards air outlet end;Air passage is also equipped with the second sealing surface around aerator peripheral side;Aeration device also includes, peripheral edge sealing part, around setting in aerator peripheral side, to form sealing between the two by respectively with aerator peripheral edge surface and the second sealing surface abuts;Inner sealing part, to form sealing between the two by respectively with aerator outer surface and the first sealing surface abuts, inner sealing part is sealed with peripheral edge sealing part Connection, and along the radial direction of aerator from peripheral edge sealing part to its central position extends.The utility model is equipped with receiving part in air passage, so that receiving part not only can support aerator, to facilitate aerator installation, can also increase the contact area between air passage and aerator, to further increase the gap length generated between aerator and air passage, so that it can be in the gap between aerator peripheral edge surface and the second sealing surface on the inner side wall of air passage Setting peripheral edge sealing part, to fill the gap vertically extending between aerator peripheral edge surface and the second sealing surface, to seal between the two;Also can be in the gap between aerator outer surface and the first sealing surface on receiving part Setting inner sealing part, to fill the gap horizontally extending between aerator outer surface and the first sealing surface, to seal between the two, namely, peripheral edge sealing part seals between aerator peripheral edge surface and air passage, inner sealing part seals between aerator outer surface and air passage, so that the aerator embedded in air passage has double sealing, inner sealing part seals between aerator and air passage for the first time, peripheral edge sealing part seals between aerator and air passage for the second time, greatly improve the sealing effect between aerator and air passage, because peripheral edge sealing part and inner sealing part are sealed connection, so that peripheral edge sealing part and inner sealing part form continuous filling between the gap of air passage and aerator, so that the sealing path of peripheral edge sealing part and inner sealing part for aerator is continuous without interruption, further improve the sealing effect.The inner sealing part and the peripheral sealing part are beneficial to prevent the gas in the gas channel from escaping from the gas channel through the gap between the aeration disc and the gas channel to form large-sized escape bubbles, because the large-sized escape bubbles are slow to diffuse and are extremely easy to be diffused away from the center of the stirring device under the pushing of the impeller, resulting in that the escape bubbles cannot be fully dispersed by the impeller or attached to the impeller, and causing the fluctuation of the KLa value, therefore, the setting of the inner sealing part and the peripheral sealing part makes the gas in the gas channel only escape from the aeration disc to form small-sized diffusion bubbles with fast flow speed, so that the diffusion bubbles flow to the impeller quickly and are dispersed by the impeller to be transmitted to each part of the bag body, and the stability of the KLa value is ensured.

[0040] The technical solutions of the embodiments of the utility model will be explained and described below in combination with the drawings of the embodiments of the utility model. However, the following embodiments are only preferred embodiments of the utility model, not all. Based on the embodiments in the embodiments, other embodiments obtained by the person skilled in the art without creative labor all belong to the protection scope of the utility model.

[0041] In the description of the utility model, it is understood that the orientation or position relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "clockwise", "counterclockwise" and the like are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, and cannot be understood as indicating or implying that the indicated device or element must have a particular orientation, a particular orientation and operation, therefore, cannot be understood as a limitation on the utility model.

[0042] In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more features. In the description of the utility model, unless otherwise specified, the meaning of "multiple" is two or more, unless otherwise explicitly limited.

[0043] In the utility model, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood in a broad sense, for example, can be fixedly connected, or can be detachably connected, or integrally connected, can be mechanically connected, or electrically connected, can be directly connected, or indirectly connected through an intermediate medium, can be the communication inside two elements. For the person skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0044] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0045] Example 1:

[0046] A bioreactor, such as Figures 1 to 7 As shown, the device includes a stirring device, an aeration device, and a bag. The bag is made of a flexible material known to those skilled in the art, and will not be described in detail here. The stirring device includes a rotatable impeller 100 located inside the bag. The aeration device includes a base 400 and an aeration disc 600. The base 400 is positioned (e.g., by welding) on ​​the bag. In this embodiment, the base 400 is an integral structure. In other embodiments, multiple through holes may be opened on the bag, and the base 400 may be set as multiple independent components, with each base 400 welded to a different through hole.

[0047] In this embodiment, the base 400 includes an inner surface 410 and an outer surface 420. The inner surface 410 faces the impeller 100, and the outer surface 420 faces away from the impeller 100. An air passage 500 is provided on the base 400, having an inlet end 511 for air intake and an outlet end 521 for air exhaust. In this embodiment, as shown... Figure 4 As shown, the air passage 500 is along the axial direction of the impeller 100 (i.e., Figure 4The gas channel 500 is arranged through the base 400 in the vertical direction (the vertical direction in the figure), and the end of the gas channel 500 away from the impeller 100 is the air inlet end 511, and the end of the gas channel 500 toward the impeller 100 is the air outlet end 521. Of course, it can be understood that in other embodiments, the gas channel 500 can also be arranged in a bent manner or have other shapes, that is, the air inlet end 511 of the gas channel 500 and the air outlet end 521 of the gas channel 500 can be arranged on the same face or different faces of the base 400, as long as the air outlet end 521 of the gas channel 500 is arranged toward the impeller 100. The aerator disc 600 is embedded in the gas channel 500, and the aerator disc 600 has an aerator disc inner surface 610, an aerator disc outer surface 620, and an aerator disc peripheral surface 630. The aerator disc inner surface 610 is arranged toward the impeller 100, the aerator disc outer surface 620 is arranged away from the impeller 100, and the aerator disc peripheral surface 630 is arranged at the periphery of the aerator disc 600. The aerator disc 600 is wholly or partially located in the gas channel 500, that is, the aerator disc inner surface 610 can be arranged higher than the base inner surface 410, can be flush with the base inner surface 410, or can be lower than the base inner surface 410.

[0048] As Figure 3The gas channel 500 is also provided with a receiving portion 540 for receiving the aeration disc 600. In the embodiment, the gas channel 500 is divided into two sections, i.e. a first gas guiding section 510 and a second gas guiding section 520 along the gas flow direction. The first gas guiding section 510 is arranged away from the impeller 100, and the second gas guiding section 520 is arranged close to the impeller 100. That is, the gas first passes through the first gas guiding section 510 and then passes through the second gas guiding section 520 in the gas channel 500. The receiving portion 540 can be arranged at any position in the gas channel 500. In the embodiment, the inner diameter of the second gas guiding section 520 is greater than that of the first gas guiding section 510, so that a stepped receiving portion 540 is formed at the connection between the second gas guiding section 520 and the first gas guiding section 510. Thus, the receiving portion 540 is arranged at the portion of the gas channel 500 close to the impeller 100, so as to facilitate the installation and removal of the aeration disc 600. Meanwhile, the stepped receiving portion 540 has high strength and is not easy to break, and has good supporting effect on the aeration disc 600. Of course, in some other embodiments, the first gas guiding section 510 and the second gas guiding section 520 can have the same inner diameter, and the receiving portion 540 is formed by an annular protruding rib protruding from the inner side wall of the gas channel. The annular protruding rib is arranged in the first gas guiding section 510 and surrounds the central axis of the gas channel 500, so as to facilitate production and processing and have good gas guiding effect. The receiving portion 540 has a first sealing surface 541 arranged towards the gas outlet end 521. The gas channel 500 is also provided with a second sealing surface 530 arranged around the peripheral surface 630 of the aeration disc. The aeration device further comprises a peripheral sealing portion 700 and an inner sealing portion 800. The peripheral sealing portion 700 is arranged around the peripheral side of the aeration disc 600 and abuts against the peripheral surface 630 of the aeration disc and the second sealing surface 530, so as to form a seal therebetween. The inner sealing portion 800 is sealingly connected with the peripheral sealing portion 700 (the sealing connection herein includes that the inner sealing portion 800 and the peripheral sealing portion 700 are separate and sealingly abut against each other, and also includes that the inner sealing portion 800 and the peripheral sealing portion 700 are integrated). The inner sealing portion 800 extends from the peripheral sealing portion 700 to the central position of the aeration disc 600 along the radial direction of the aeration disc 600. The inner sealing portion 800 abuts against the outer surface 620 of the aeration disc and the first sealing surface 541, so as to form a seal therebetween. The gas in the gas channel 500 escapes from the aeration disc 600 to form diffusion bubbles flowing towards the impeller 100. In the embodiment, the aeration of the aeration disc 600 can be realized by a plurality of gas holes penetrating the aeration disc 600, or the aeration disc 600 can be made of sintered sheets having a gas permeable function.

[0049] The utility model discloses a support 540 is arranged in the air channel 500, makes the support 540 not only can support the aeration disc 600 to facilitate the aeration disc 600 installation, can also increase the contact area between air channel 500 and aeration disc 600, and then increase the gap length between aeration disc 600 and air channel 500, make can set up the circumferential sealing part 700 in the gap between aeration disc peripheral surface 630 and the second sealing surface 530 on the inner wall of air channel 500, fill the gap between aeration disc peripheral surface 630 and the second sealing surface 530 and seal between both, can also set up the inner sealing part 800 in the gap between aeration disc outer surface 620 and the first sealing surface 541 on the support 540, fill the gap between aeration disc outer surface 620 and the first sealing surface 541 and seal between both, that is, the circumferential sealing part 700 seals between aeration disc peripheral surface 630 and air channel 500, the inner sealing part 800 seals between aeration disc outer surface 620 and air channel 500, makes the aeration disc 600 embedded in air channel 500 has double sealing, the inner sealing part 800 has primary sealing between aeration disc 600 and air channel 500, the circumferential sealing part 700 has secondary sealing between aeration disc 600 and air channel 500, greatly improves the sealing effect between aeration disc 600 and air channel 500, and because the circumferential sealing part 700 and the inner sealing part 800 are sealedly connected, so the circumferential sealing part 700 and the inner sealing part 800 form continuous filling to the gap between air channel 500 and aeration disc 600, make the sealing path of circumferential sealing part 700 and the inner sealing part 800 continuous without interruption to aeration disc 600, further improve the sealing effect.

[0050] As Figure 4 And 5As shown, the extension length of the inner sealing portion 800 along the radial direction of the aerator plate 600 is L1, and the extension length of the receiving portion 540 along the radial direction of the aerator plate 600 is L2. In this embodiment, L1>L2, that is, the extension length of the inner sealing portion 800 along the radial direction of the aerator plate 600 is greater than the extension length of the receiving portion 540 along the radial direction of the aerator plate 600. By setting the extension length of the inner sealing portion 800 along the radial direction of the aerator plate 600 to be greater than the extension length of the receiving portion 540 along the radial direction of the aerator plate 600, the inner sealing portion 800 and the receiving portion 540 are completely attached to the entire first sealing surface 541. This not only maximizes the sealing between the inner sealing portion 800 and the receiving portion 540, but also increases the sealing area between the inner sealing portion 800 and the aerator plate 600, thereby enhancing the sealing effect between the inner sealing portion 800 and the aerator plate 600 and preventing the gas in the gas channel 500 from escaping between the inner sealing portion 800 and the receiving portion 540 or between the inner sealing portion 800 and the aerator plate 600. Preferably, 0.8≤L1 / L2≤1.5. By setting L1 / L2 in the range of 0.8≤L1 / L2≤1.5, the inner sealing portion 800 prolongs the bubble escape time by prolonging the sealing path of the outer edge of the aerator plate 600, which promotes the further escape of most bubbles through the aerator plate, thereby increasing the number of diffusion bubbles and facilitating the sufficient dispersion of the diffusion bubbles, which ultimately helps to stabilize the KLa value.

[0051] The first turbulence portion 900 is provided on the inner surface 610 of the aerator plate in this embodiment. The first turbulence portion 900 is arranged at a position close to the outer edge of the inner surface 610 of the aerator plate to promote the turbulence of the fluid at this position and increase the turbulent flow of the liquid at this position, thereby allowing the diffusion bubbles generated by the aerator plate 600 to be promptly broken up and dispersed in the bag body at this position, which helps to improve the stability of the KLa value. The first turbulence portion 900 can be a continuous structure or arranged intermittently, and can be a protruding structure protruding from the inner surface 610 of the aerator plate or a recessed structure provided on the inner surface 610 of the aerator plate. The end surface of the first turbulence portion 900 facing the impeller 100 is a turbulence surface 910. In this embodiment, the turbulence surface 910 protrudes from the inner surface 610 of the aerator plate, that is, the first turbulence portion 900 protrudes from the inner surface 610 of the aerator plate, and the first turbulence portion 900 is a continuous annular structure. The first turbulence portion 900 is continuously arranged on the diffusion path of the diffusion bubbles and protrudes from the inner surface 610 of the aerator plate, so that a large number of diffusion bubbles can collide with the first turbulence portion 900 and break up, thereby achieving better dispersion effect.

[0052] The first turbulence portion 900 can be integrally arranged with the aerator disc 600, or can be a separate structure from the aerator disc 600. In the present embodiment, the first turbulence portion 900 is arranged separately from the aerator disc 600. The first turbulence portion 900 extends along the radial direction of the aerator disc 600 and is integrally arranged with the peripheral sealing portion 700. That is, the first turbulence portion 900 abuts against the outer periphery of the inner surface 610 of the aerator disc 600 to seal the inner surface 610 of the aerator disc 600. The outer periphery of the first turbulence portion 900 is connected to the peripheral sealing portion 700, so that the first turbulence portion 900, the peripheral sealing portion 700 and the inner sealing portion 800 jointly form the sealing groove 900. The peripheral edge of the aerator disc 600 is embedded in the sealing groove 900. The first turbulence portion 900 not only increases the turbulent flow on the aerator disc 600, but also extends the sealing area between the inner sealing portion 800, the peripheral sealing portion 700 and the aerator disc 600. The first turbulence portion 900, the peripheral sealing portion 700 and the inner sealing portion 800 jointly wrap around the peripheral edge of the aerator disc 600, and form a continuous and uninterrupted sealing path around the peripheral edge of the aerator disc 600. This is conducive to improving the sealing effect, and also avoids the inner sealing portion 800 and the peripheral sealing portion 700 from being separated from the aerator disc 600, thereby enhancing the sealing effect of the peripheral edge of the aerator disc 600 and preventing the gas in the gas channel 500 from escaping between the aerator disc 600 and the gas channel 500.

[0053] In the present embodiment, the first turbulence portion 900 at least satisfies one of the following four conditions. That is, the first turbulence portion 900 can satisfy one of the following four conditions, or a combination of any two of the following conditions, or a combination of any three of the following conditions, or all of the following four conditions.

[0054] Condition (1) provides that the distance between the turbulence surface 910 and the inner surface 410 of the base in the axial direction of the aerator disc 600 is D1, the distance between the turbulence surface 910 and the inner surface 610 of the aerator disc in the axial direction of the aerator disc 600 is D2, and 1.5≤D1 / D2≤5 is satisfied. If the ratio of D1 and D2 is too small, the first turbulence portion 900 has poor turbulence effect on the fluid. If the ratio of D1 and D2 is too large, the height of the first turbulence portion 900 is too high, which easily interferes with the rotation of the impeller 100. By controlling the ratio of D1 and D2 to be within the range of 1.5≤D1 / D2≤5, the rotation of the impeller 100 is prevented from being interfered by the first turbulence portion 900, and the turbulence effect is improved.

[0055] In case (2), it is provided that the distance between the spoiler surface 910 and the inner surface 610 of the aerator disc 600 in the axial direction of the aerator disc 600 is D2, and the effective gas outlet area of the inner surface 610 of the aerator disc is S; 0.05≤D2 / S≤1 is satisfied. The effective gas outlet area of the inner surface 610 of the aerator disc refers to the area of the region exposed inside the bag body and available for gas to pass through without being blocked by any obstruction. If the ratio of D2 to S is too large, it means that the distance between the spoiler surface 910 and the inner surface 610 of the aerator disc 600 in the axial direction of the aerator disc 600 is too large, i.e., the first spoiler portion 900 is too high, and in the actual use or processing process, the base 400 will bend towards the impeller 100, so that the high first spoiler portion 900 will interfere with the rotation of the impeller 100. If the ratio of D2 to S is too small, it means that the distance between the spoiler surface 910 and the inner surface 610 of the aerator disc 600 in the axial direction of the aerator disc 600 is too small, so that the first spoiler portion 900 does not disturb the inner surface 610 of the aerator disc sufficiently, resulting in a small amount of turbulence near the inner surface 610 of the aerator disc. By controlling the ratio of D2 to S within the range of 0.05≤D2 / S≤1, the amount of turbulence of the inner surface 610 of the aerator disc can be increased without interfering with the rotation of the impeller 100, so that the diffusion bubbles can be dispersed and rapidly diffused on the inner surface 610 of the aerator disc.

[0056] In case (3), it is provided that the thickness of the inner sealing portion 800 in the axial direction of the aerator disc 600 is H1, and the thickness of the first spoiler portion 900 in the axial direction of the aerator disc 600 is H2, and 0.3≤H1 / H2≤3 is satisfied. If the ratio of H1 to H2 is too large, it means that the axial height of the first spoiler portion 900 is too small, resulting in poor disturbance effect of the first spoiler portion 900. If the ratio of H1 to H2 is too small, it means that the axial height of the inner sealing portion 800 is too small, and the deformation amount of the thin inner sealing portion 800 is small. If the aerator disc 600 is loose and moves away from the receiving portion 540, the inner sealing portion 800 cannot compensate for the excess gap between them by a certain amount of deformation, so that the sealing effect between the aerator disc 600 and the receiving portion 540 is affected, resulting in poor sealing effect between the aerator disc 600 and the receiving portion 540. By controlling the ratio of H1 to H2 within the range of 0.3≤H1 / H2≤3, the sealing between the aerator disc 600 and the receiving portion 540 can be ensured while the disturbance effect of the first spoiler portion 900 is enhanced.

[0057] In case (4), the inner sealing part 800 has a width L1 along the radial direction of the aerator disc 600, and the first turbulence part 900 has a width L3 along the radial direction of the aerator disc 600, and 0.5≤L1 / L3≤3 is satisfied. If the ratio of L1 to L3 is too large or too small, the inner sealing part 800 will excessively shield the aerator disc 600, thereby reducing the effective air inlet area of the outer surface 620 of the aerator disc, and the effective air inlet area of the outer surface 620 of the aerator disc refers to the area of the outer surface 620 of the aerator disc that is not shielded by any shielding object and can be used for gas to pass through. The reduction of the effective air inlet area of the outer surface 620 of the aerator disc will result in that the gas in the gas channel 500 cannot flow out of the aerator disc 600 quickly, and the gas pressure in the gas channel 500 is increased. By controlling the ratio of L1 to L3 to be within the range of 0.5≤L1 / L3≤3, it can be ensured that the gas flow passes through the aerator disc 600 quickly, and the gas pressure in the gas channel 500 is prevented from being increased.

[0058] In the embodiment, the second turbulence part 430 is arranged on the inner surface 410 of the base, and the second turbulence part 430 can be protruded from the inner surface 410 of the base or can be a groove arranged on the inner surface 410 of the base. The second turbulence part 430 can be arranged continuously or discontinuously, and preferably, the second turbulence part 430 is arranged around the peripheral sealing part 700 to promote the turbulence of the fluid at the position and increase the turbulent flow of the liquid at the position, so as to assist the impeller 100 to disperse the diffusion bubbles generated by the aerator disc 600 in time and improve the stability of the KLa value.

[0059] In the embodiment, the second turbulence part 430 is arranged on the inner surface 410 of the base, and the second turbulence part 430 can be protruded from the inner surface 410 of the base or can be a groove arranged on the inner surface 410 of the base. The second turbulence part 430 can be arranged continuously or discontinuously, and preferably, the second turbulence part 430 is arranged around the peripheral sealing part 700 to promote the turbulence of the fluid at the position and increase the turbulent flow of the liquid at the position, so as to assist the impeller 100 to disperse the diffusion bubbles generated by the aerator disc 600 in time and improve the stability of the KLa value.

[0060] The distance between the turbulence surface 910 and the inner surface 410 of the base along the axial direction of the aeration disc 600 is D1, and the distance between the turbulence surface 910 and the end face of the second turbulence part 430 facing the impeller 100 along the axial direction of the aeration disc 600 is D3. D1 and D3 satisfy the following relationship: 0.1≤D3 / D1≤2. Because when the distance D3 between the end face of the second turbulence part 430 facing the impeller 100 and the turbulence surface 910 along the axial direction of the aeration disc 600 increases, the distance D1 between the turbulence surface 910 and the inner surface 410 of the base along the axial direction of the aeration disc 600 also increases, and the two are positively correlated. Therefore, setting the ratio of D3 to D1 in the range of 0.1≤D3 / D1≤2 can take into account turbulence and avoid interference between the heights of the first turbulence part 900 and the second turbulence part 430 and the impeller 100.

[0061] like Figure 1 and Figure 6 As shown, in this embodiment, a base plate 200 is provided inside the bag body, and the base plate 200 can also be welded to the bag body 200. The impeller 100 is rotatably connected to the base plate 200. The base 400 and the base plate 200 are separately arranged. The base 400 is annular and surrounds the base plate 200. The inner diameter of the base 400 is larger than the outer diameter of the base plate 200, so that a turbulence gap is formed between the inner sidewall of the base 400 and the outer sidewall of the base plate 200. A flexible member 320 is connected between the base plate 200 and the base 400. The flexible member 320 provides a turbulence gap away from the impeller 100. The end is closed to form a turbulence groove 310 with its opening facing the impeller 100. There is a height difference between the inner bottom surface of the turbulence groove 310 and the inner surface 410 of the base, so that the fluid flowing through the turbulence groove 310 will form turbulence near the turbulence groove 310, increasing the turbulent flow rate near the turbulence groove 310 and enhancing the bubble breaking effect near the turbulence groove 310. The setting of the flexible member 320 allows for a certain amount of movement between the substrate 200 and the base 400. The relative movement between the two can also generate turbulence on the fluid, promote the generation of turbulence, and further improve the bubble breaking effect. Of course, it is understood that the above method is only a preferred solution of this embodiment. In other embodiments, the turbulence groove 310 facing the impeller 100 can be directly formed on the substrate 200, or the turbulence groove 310 facing the impeller 100 can be directly formed on the base 400, or the base 400 and the substrate 200 can be integrally formed, and the turbulence groove 310 facing the impeller 100 can be formed at any position such as the base 400, the substrate 200, or the connection between the base 400 and the substrate 200.

[0062] In the embodiment, the impeller 100 comprises an impeller shaft 110 and a plurality of (two or more) blades 120 distributed along the circumference of the impeller shaft 110, the blade 120 comprises a mounting end 121 connected with the impeller shaft 110 and a free end 122, the free end 122 is an end of the blade 120 away from the mounting end 121, the impeller shaft 110 drives the plurality of blades 120 to rotate to agitate the fluid in the bag, the blade 120 in the embodiment is obliquely arranged, the side of the blade 120 close to the aeration device is a stirring edge 123, the stirring edge 123 is between the mounting end 121 and the free end 122, a part of the stirring edge 123 is provided with a turbulent flow part (not shown in the figure), the turbulent flow part is zigzag-shaped to enhance the turbulent flow effect of the stirring edge 123, so that the diffusion bubbles generated by the aeration disc 600 can be dispersed and diffused in time by the impeller 100, to ensure the stability of the KLa value. Preferably, as shown in Figure 2 the turbulent flow part is concentratedly arranged at the position opposite to the stirring edge 123 and the aeration disc 600, that is, taking the plane perpendicular to the impeller shaft 110 as a projection plane, the projections of the base 400 and the turbulent flow groove 310 on the projection plane all fall into the projection on the projection plane of the rotation area of the turbulent flow part, which is beneficial to the diffusion bubbles generated by the aeration disc 600 to flow along the axial direction of the impeller 100 after leaving the aeration disc 600, by concentratingly arranging the turbulent flow part at the position opposite to the stirring edge 123 and the aeration disc 600, the turbulent flow part of the impeller 100 can form a large amount of turbulent flow on the path of the diffusion bubbles during the rotation of the impeller 100, so as to disperse the diffusion bubbles and assist the rapid diffusion of the diffusion bubbles. Of course, the blade 120 can be arranged in various shapes, and blades 120 of various shapes and sizes can be suitable for the stirring device.

[0063] Embodiment two:

[0064] As shown in Figure 7 the embodiment, the difference between the embodiment and the embodiment one is that in the embodiment, the second turbulent flow part 430 is arranged on the inner surface 410 of the base and is recessed into the base 400, the distance between the end surface of the second turbulent flow part 430 facing the impeller and the turbulent flow surface 910 in the radial direction of the aeration disc 600 is D4, and 5≤D4 / D1≤10 is satisfied. By setting the ratio of D4 and D1 in the range of 5≤D4 / D1≤10, the turbulent flow can be considered and the height of the first turbulent flow part 900 and the second turbulent flow part 430 can be avoided to interfere with the impeller 100.

[0065] The above merely is preferred implementation manner of the present application, the protection scope of the present application is not only limited to the above examples, and belongs to the technical scheme under the idea of the present application all belongs to the protection scope of the present application. It should be pointed out that, for ordinary skilled person in the art, under the premise of not departing from the principle of the present application, some improvements and decorations, these improvements and decorations should also be considered as the protection scope of the present application.

Claims

1. A bioreactor comprising a stirring device, an aeration device and a bag made of flexible material, the stirring device comprising an impeller arranged in the bag, the aeration device comprising: a base arranged in the bag, comprising a base inner surface facing the impeller and a base outer surface facing away from the impeller; an air channel arranged on the base, having an air inlet end and an air outlet end arranged towards the impeller; an aeration disc at least partially embedded in the air channel, having an aeration disc inner surface facing the impeller, an aeration disc outer surface facing away from the impeller and an aeration disc peripheral surface arranged at the peripheral edge of the aeration disc; characterized in that: a receiving portion is arranged in the air channel, the receiving portion having a first sealing surface facing the air outlet end; a second sealing surface is arranged around the peripheral edge of the aeration disc; the aeration device further comprises: a peripheral sealing portion arranged around the peripheral edge of the aeration disc, forming a seal between the aeration disc peripheral surface and the second sealing surface; and an inner sealing portion arranged to form a seal between the aeration disc outer surface and the first sealing surface, the inner sealing portion being sealingly connected to the peripheral sealing portion and extending from the peripheral sealing portion to the center of the aeration disc along the radial direction of the aeration disc; the radial extension length of the inner sealing portion is greater than the maximum radial extension length of the receiving portion; the radial extension length of the inner sealing portion along the aeration disc is L1, and the radial extension length of the receiving portion along the aeration disc is L2, satisfying 0.8≤L1 / L2≤1.5; the air channel comprises, in sequence along the gas flow direction, a first gas guide section away from the impeller and a second gas guide section close to the impeller, the inner diameter of the second gas guide section is greater than the inner diameter of the first gas guide section, so as to form the receiving portion at the connection between the second gas guide section and the first gas guide section; or, an annular protruding rib is arranged on the air channel, protruding from the inner side wall of the air channel, the annular protruding rib is arranged around the central axis of the air channel, so as to form the receiving portion; the first turbulence portion is arranged at a position close to the outer edge of the aeration disc inner surface; the end surface of the first turbulence portion facing the impeller is a turbulence surface, the turbulence surface protrudes from the aeration disc inner surface; (1) the distance between the turbulence surface and the base inner surface along the axial direction of the aeration disc is D1, and the distance between the turbulence surface and the aeration disc inner surface along the axial direction of the aeration disc is D2, satisfying 1.5≤D1 / D2≤5; (2) the distance between the turbulence surface and the aeration disc inner surface along the axial direction of the aeration disc is D2, and the effective air outlet area of the aeration disc inner surface is S, satisfying 0.05≤D2 / S≤1; (3) the thickness of the inner sealing portion along the axial direction of the aeration disc is H1, and the thickness of the first turbulence portion along the axial direction of the aeration disc is H2, satisfying 0.3≤H1 / H2≤3; (4) the width of the inner sealing portion along the radial direction of the aeration disc is L1, and the width of the first turbulence portion along the radial direction of the aeration disc is L3, satisfying 0.5≤L1 / L3≤3; the first turbulence portion at least satisfies one of the above four conditions. ​ ​ ​ ​ ​ ​ ​ ​ 2. A bioreactor according to claim 1, characterized in that: ​ 3. A bioreactor according to claim 2, wherein: ​ 4. The bioreactor of claim 1, wherein: ​ 5. The bioreactor of claim 1, wherein: ​ 6. A bioreactor according to claim 5, wherein: ​ 7. A bioreactor according to claim 6, wherein: ​ ​ ​ ​ ​ 8. A bioreactor according to any of claims 6-7, c h a ra cte ri zed i n that: The second turbulence part is arranged on the inner surface of the base and surrounds the peripheral sealing part.

9. A bioreactor according to claim 8, wherein: The second turbulence part is arranged on the inner surface of the base and surrounds the peripheral sealing part.

10. The bioreactor of claim 6, wherein: The first turbulence part extends along the radial direction of the aerator and abuts against the inner surface of the aerator to seal the space therebetween.

11. The bioreactor of claim 1, wherein: The base is provided with a turbulence groove, and the opening of the turbulence groove faces the impeller; or the bag body is provided with a base plate, the impeller is rotationally connected to the base plate, the base plate is provided with a turbulence groove, and the opening of the turbulence groove faces the impeller; or the bag body is provided with a base plate, the impeller is rotationally connected to the base plate, the base and the base plate are an integral structure, a turbulence groove is arranged at the connection position of the base and the base plate, and the opening of the turbulence groove faces the impeller; or the bag body is provided with a base plate, the impeller is rotationally connected to the base plate, the base and the base plate are a split structure, the base surrounds the base plate, the inner diameter of the base is greater than the outer diameter of the base plate, a turbulence gap is formed between the base plate and the base, a flexible member is connected between the base plate and the base, the flexible member seals one end of the turbulence gap away from the impeller, and a turbulence groove with an opening facing the impeller is formed.

12. A bioreactor according to claim 11, wherein: The impeller comprises an impeller shaft and a plurality of blades, the plurality of blades are distributed along the circumferential direction of the impeller shaft, the blade comprises a mounting end connected to the impeller shaft and a free end away from the impeller shaft, and the side of the blade close to the aerator is a stirring edge connected between the mounting end and the free end.

13. A bioreactor according to claim 12, wherein: The projection of the base and the turbulence groove on the projection plane perpendicular to the impeller shaft falls within the projection of the rotating area of the turbulence part on the projection plane.

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