Immune cell culture device

Abstract

The utility model relates to the technical field of cell culture devices, in particular to an immune cell culture device which comprises a culture box main body, one side of the culture box main body is rotatably connected with a sealing door, one end of the culture box main body is slidably connected with a fixing mechanism, the fixing mechanism comprises a telescopic column and penetrates into the culture box main body, and the telescopic column is connected with the sealing door. A sealing door is arranged in the incubator body and located on the side, away from the sealing door, of the incubator body, an isolation mechanism is fixedly connected to the interior of the incubator body and comprises a plurality of supporting plates, and the supporting plates are fixedly connected to the inner wall of the incubator body. According to the incubator disclosed by the utility model, limiting and locking of internal parts of the isolation mechanism can be relieved through mutual matching of the switch sealing door and the internal parts of the fixing mechanism, and culture dishes of the incubator main body can be distinguished and isolated through mutual matching of the internal parts of the isolation mechanism; therefore, the influence of frequent opening and closing of the sealing door on cell culture dishes in the incubator main body is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture device technical field, concretely relates to an immune cell culture device. BACKGROUND

[0002] Immune cells refer to cells participating in or related to immune response, including lymphocytes, dendritic cells, monocytes or macrophages, granulocytes, mast cells, etc., immune cells can be divided into many kinds, various immune cells play an important role in the human body, immune cells are commonly known as white blood cells, including innate lymphocytes, various phagocytes and lymphocytes capable of recognizing antigens and producing specific immune response, therefore, the research on immune cells is extremely important.

[0003] Cell culture refers to a method of simulating in-vivo environment in vitro to make cells survive, grow, reproduce and maintain main structure and function, cell culture generally includes adherent culture, suspension culture and immobilized culture, wherein adherent culture refers to culture of cells adhering to a certain solid surface, cells spread rapidly after adhering, then start mitosis and enter logarithmic growth phase soon, generally spread on the surface of culture vessel and form dense cell monolayer after several days.

[0004] With the continuous development of cell culture and monitoring technology, the existing cell culture generally completes preliminary culture of immune cells in a sterile room and transplants into a culture dish, and the culture dish is placed in a culture device for a period of time for sampling observation after being cultured and divided by cells themselves, but in the use of the existing culture device, due to the different growth periods of cells in the culture dish, the frequent opening and closing of the culture device when the experimenters sample and observe the cells in different culture dishes in the culture device will affect the culture effect of the cells in the culture dish, thereby affecting the experimental effect.

[0005] Therefore, it is necessary to provide an immune cell culture device to solve the above problems. UTILITY MODEL CONTENT

[0006] The utility model aims at providing an immune cell culture device, through the mutual cooperation between the sealing door and the internal parts of the fixing mechanism, the limiting locking of the internal parts of the isolation mechanism can be released, through the mutual cooperation between the internal parts of the isolation mechanism, the culture dishes of the culture box main body can be distinguished and isolated, thereby reducing the influence of frequent opening and closing of the sealing door on the culture dishes in the culture box main body, to solve the problem that in the prior art, due to the different growth periods of cells in the culture dish, the frequent opening and closing of the culture device when the experimenters sample and observe the cells in different culture dishes in the culture device will affect the culture effect of the cells in the culture dish, thereby affecting the experimental effect.

[0007] In order to achieve the above object, the utility model provides following technical scheme: an immune cell culture device, the one side of culture box main part is rotatively connected with sealed door, the one end of culture box main part is slidably connected with fixed mechanism, and it is through to the inside of culture box main part, and it is located the one side of culture box main part away from sealed door, the inside of culture box main part is connected with the fixed isolation mechanism of wall, and it is located the inner wall of culture box main part.

[0008] Preferably, the fixed mechanism includes telescopic column, the telescopic column has a plurality of, a plurality of the telescopic column is located the one side of culture box main part away from sealed door, and it is slidably through to the inside of culture box main part, the one end of telescopic column is connected with the fixed telescopic spring that is through to the inside of culture box main part, the one side of telescopic column is slidably connected with conical block close to sealed door, and it is located the inside of culture box main part, the one end of conical block is connected with the fixed limit stop that is close to sealed door, the one side of limit stop is connected with the fixed limit spring that is located the one end of conical block.

[0009] Preferably, the isolation mechanism includes a plurality of support plates, a plurality of the support plates are connected and fixed to the inner wall of the culture box main part, and are located between the sealed door and the telescopic column, the one end is attached with a isolation plate, and is rotatably connected to the inner wall of the culture box main part, the one end of the support plate close to the isolation plate is installed and fixed with a rebounder, and penetrates into the inside of the support plate, the one end of the rebounder penetrating into the inside of the connecting column is slidably connected with the connecting column, the top end of the support plate is slidably connected with a tray, the bottom end of the tray is connected and fixed with a sliding block, and penetrates into the inside of the support plate, the outer wall of the sliding block is connected and fixed with a damping block on both sides, the one end of the sliding block away from the rebounder is connected and fixed with a support spring, and is located in the inside of the support plate, the bottom end of the connecting column is connected and fixed with a C-shaped block, and penetrates into the one end of the sliding block through the connecting column, the bottom end of the C-shaped block is connected and fixed with a return spring.

[0010] Preferably, the inside of the culture box main part is provided with a connecting groove matched with the telescopic column, the sealed door is rotatably connected with the culture box main part through a hinge, and the telescopic column is provided with a conical groove matched with the conical block on one side.

[0011] Preferably, the one side of the isolation plate close to the conical block is provided with a fixing groove matched with the conical block, the isolation plate is rotatably connected with the culture box main part through a hinge, the top end of the support plate is provided with a sliding groove matched with the sliding block, and the sliding groove is provided with a damping groove matched with the damping block on both sides.

[0012] Preferably, the inside of the support plate is provided with a support groove matched with the C-shaped block, the inside of the connecting column is provided with a limiting groove matched with the C-shaped block, and the limiting groove in the inside of the connecting column and the top end of the C-shaped block are both provided with a circular arc surface.

[0013] In the above technical solutions, the technical effects and advantages provided by the utility model are as follows:

[0014] 1. By opening the sealing door, the sealing door is separated from the incubator main body, the sealing door releases the extrusion on the telescopic column, the telescopic column releases the extrusion on the telescopic spring, the telescopic spring pushes the telescopic column to reset, the telescopic column moves inside the incubator main body to release the extrusion on the conical block, the conical block drives the limiting plate to release the extrusion on the limiting spring, the limiting spring resets to push the limiting plate to move, the limiting plate moves to drive the conical block to move, the conical block moves out of the isolation plate, and the rotation locking of the isolation plate is released.

[0015] 2. By releasing the rotation locking of the isolation plate by the conical block, the isolation plate is pushed to rotate and extrude the rebounder, the rebounder is pushed out to push the isolation plate to move and release the isolation operation on the support plate, the rebounder is pushed out to drive the connecting column to move, the connecting column moves to extrude the C-shaped block, the C-shaped block extrudes the reset spring to shrink and move, the C-shaped block moves to release the sliding limiting operation on the sliding block, the supporting spring pushes the sliding block to move, the isolation plate is opened, the isolation plate drives the isolation operation on the top tray of the support plate to be released, the sliding block moves through the damping blocks on both sides to drive the tray to slide off the support plate, so that the staff can select the culture dishes on the tray, the staff can select the culture dishes on different support plates according to the needs, and the unselected support plates are isolated and sealed by the isolation plate, so that the influence of the opening of the sealing door on the culture dishes is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments or prior art, the drawings needed in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments described in the present application, and other drawings can also be obtained by those skilled in the art according to these drawings.

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a schematic diagram of the cross-sectional structure of the incubator main body of the utility model;

[0019] Figure 3 It is a schematic diagram of the connecting structure of the isolation plate of the utility model;

[0020] Figure 4 It is a schematic diagram of the cross-sectional structure of the support plate of the utility model;

[0021] Figure 5 It is a schematic diagram of the Figure 2 The enlarged structure of position A in the middle. The enlarged structure of position A in the middle.

[0022] Reference signs:

[0023] 1, incubator main body; 101, sealing door; 2, fixing mechanism; 201, telescopic column; 202, telescopic spring; 203, conical block; 204, limiting plate; 205, limiting spring; 3, isolation mechanism; 301, support plate; 302, isolation plate; 303, rebounder; 304, connecting column; 305, tray; 306, sliding block; 307, damping block; 308, supporting spring; 309, C-shaped block; 310, reset spring. DETAILED DESCRIPTION

[0024] In order to make the technical personnel in the art better understand the technical scheme of the utility model, the utility model will be described in further detail below with reference to the drawings.

[0025] The utility model provides a kind of immune cell culture device as Figures 1-5 As shown in the drawing, it includes incubator main body 1, one side of incubator main body 1 is rotatably connected with sealing door 101, one end of incubator main body 1 is slidably connected with fixing mechanism 2, and it is connected to the inside of incubator main body 1, and it is located at the side of incubator main body 1 away from sealing door 101, the inside of incubator main body 1 is fixedly connected with isolation mechanism 3, and it is located at the inner wall of incubator main body 1, by the mutual cooperation between the inside parts of sealing door 101 and fixing mechanism 2, the limiting locking of the inside parts of isolation mechanism 3 can be released, and by the mutual cooperation between the inside parts of isolation mechanism 3, the culture dish of incubator main body 1 can be distinguished and isolated, so as to reduce the influence of frequent opening and closing of sealing door 101 on the cell culture dish in the inside of incubator main body 1.

[0026] Referring to the drawings Figures 1-5 In the drawings, fixing mechanism 2 includes telescopic column 201, and there are multiple telescopic columns 201, multiple telescopic columns 201 are located at the side of incubator main body 1 away from sealing door 101, and are slidably connected to the inside of incubator main body 1, one end of telescopic column 201 connected to the inside of incubator main body 1 is fixedly connected with telescopic spring 202, one side of telescopic column 201 close to sealing door 101 is slidably connected with conical block 203, and it is located in the inside of incubator main body 1, one end of conical block 203 is fixedly connected with limiting plate 204, one side of limiting plate 204 is fixedly connected with limiting spring 205, and it is located at one end of conical block 203, by the mutual cooperation between the inside parts of sealing door 101 and fixing mechanism 2, the limiting locking of the inside parts of isolation mechanism 3 can be released.

[0027] Referring to the drawings Figures 1-5The isolation mechanism 3 comprises a plurality of support plates 301 fixedly connected to the inner wall of the incubator body 1 and located between the sealing door 101 and the telescopic column 201, one end of each support plate 301 is attached to an isolation plate 302 rotatably connected to the inner wall of the incubator body 1, one end of each support plate 301 close to the isolation plate 302 is fixedly installed with a rebounder 303 penetrating into the inside of the support plate 301, one end of the rebounder 303 penetrating into the inside of the connecting column 304 is slidably connected with the connecting column 304, the top end of the support plate 301 is slidably connected with a tray 305, the bottom end of the tray 305 is fixedly connected with a sliding block 306 penetrating into the inside of the support plate 301, the outer wall of the sliding block 306 is fixedly connected with a damping block 307, the end of the sliding block 306 away from the rebounder 303 is fixedly connected with a supporting spring 308 located in the inside of the support plate 301, the bottom end of the connecting column 304 is fixedly connected with a C-shaped block 309 penetrating into the sliding block 306 through the connecting column 304, the bottom end of the C-shaped block 309 is fixedly connected with a reset spring 310, through the mutual cooperation between the internal parts of the isolation mechanism 3, the culture dishes in the incubator body 1 can be distinguished and isolated, thereby reducing the influence of the frequent opening and closing of the sealing door 101 on the cell culture dishes in the incubator body 1.

[0028] Referring to the drawings Figures 1-5 The inside of the incubator body 1 is provided with a connecting groove matched with the telescopic column 201, the sealing door 101 is rotatably connected to the incubator body 1 through a hinge, and the side of the telescopic column 201 is provided with a tapered groove matched with the tapered block 203, through the rotatable connection of the sealing door 101 to the incubator body 1 through a hinge, the sealing door 101 is convenient to rotate and extrude the telescopic column 201 to move inside the incubator body 1.

[0029] Referring to the drawings Figures 1-5 The side of the isolation plate 302 close to the tapered block 203 is provided with a fixing groove matched with the tapered block 203, the isolation plate 302 is rotatably connected to the incubator body 1 through a hinge, the top end of the support plate 301 is provided with a sliding groove matched with the sliding block 306, the two sides of the sliding groove are provided with damping grooves matched with the damping blocks 307, through the fixing groove matched with the tapered block 203 on the side of the isolation plate 302 close to the tapered block 203, the tapered block 203 is convenient to limit and fix the isolation plate 302.

[0030] Referring to the drawings Figures 1-5 The inside of the support plate 301 is provided with a supporting groove matched with the C-shaped block 309, the inside of the connecting column 304 is provided with a limiting groove matched with the C-shaped block 309, the limiting groove in the inside of the connecting column 304 and the top end contact surface of the C-shaped block 309 are both arc surfaces, through the supporting groove matched with the C-shaped block 309 in the inside of the support plate 301, the C-shaped block 309 is convenient to limit the sliding block 306.

[0031] The utility model discloses a working principle:

[0032] With reference to the description accompanying drawings Figures 1-5 Through opening the sealed door 101, the sealed door 101 is separated from the incubator main body 1, the sealed door 101 removes the extrusion to the telescopic column 201, the telescopic column 201 removes the extrusion to the telescopic spring 202, the telescopic spring 202 resets the telescopic column 201, the telescopic column 201 removes the extrusion to the conical block 203 in the incubator main body 1 inside, makes the conical block 203 drive the limiting plate 204 remove the extrusion to the limiting spring 205, and the limiting spring 205 resets the limiting plate 204 and moves, and the limiting plate 204 moves and drives the conical block 203 to move, makes the conical block 203 move and remove from the inside of the isolation plate 302, can remove the rotation locking of the isolation plate 302 to the isolation plate 302;

[0033] With reference to the description accompanying drawings Figures 1-5 Through the conical block 203 and remove the rotation locking of the isolation plate 302 to the isolation plate 302, push the isolation plate 302 and rotate the extrusion rebounder 303, make the rebounder 303 pop out and push the isolation plate 302 and move and remove the isolation operation to the support plate 301, and simultaneously the rebounder 303 pops out and drives the connecting column 304 to move, makes the connecting column 304 and moves and extrudes C-shaped block 309, makes C-shaped block 309 and extrudes reset spring 310 and contracts and moves, and the C-shaped block 309 moves and removes the sliding limiting operation to the sliding block 306, makes the support spring 308 push the sliding block 306 and move, and simultaneously opens the isolation plate 302, and the isolation plate 302 drives the isolation operation to the support plate 301 top tray 305, and simultaneously the sliding block 306 moves through the damping block 307 on both sides and drives the tray 305 to slide off the support plate 301 on the support plate 301, so that the staff is convenient to the selection of the petri dish on the tray 305, that is, the staff can conveniently select the petri dish on different support plate 301 according to the demand, and simultaneously the support plate 301 not selected is isolated and sealed by the isolation plate 302, so as to reduce the influence of the sealed door 101 opening to the petri dish.

[0034] The above only describes certain exemplary embodiments of the utility model through the description mode, without doubt, for ordinary skilled person in the art, under the condition that deviating from the spirit and scope of the utility model, the described embodiment can be modified in various different modes. Therefore, the above drawing and description are illustrative in nature, and should not be understood as the limitation of the utility model claim protection scope.

Claims

1. An immune cell culture device comprising a culture cabinet main body (1), characterized in that: One side of the incubator body (1) is rotatably connected with a sealing door (101), one end of the incubator body (1) is slidably connected with a fixing mechanism (2), and penetrates into the inside of the incubator body (1) and is located on the side of the incubator body (1) away from the sealing door (101), the inside of the incubator body (1) is fixedly connected with an isolation mechanism (3) and is located on the inner wall of the incubator body (1).

2. The immune cell culture device of claim 1, wherein: The fixing mechanism (2) comprises telescopic columns (201), the telescopic columns (201) are a plurality of, the plurality of telescopic columns (201) are located on the side of the incubator body (1) away from the sealing door (101) and slidably penetrate into the inside of the incubator body (1), one end of the telescopic column (201) penetrating into the inside of the incubator body (1) is fixedly connected with a telescopic spring (202), the side of the telescopic column (201) close to the sealing door (101) is slidably connected with a conical block (203) and is located in the inside of the incubator body (1), one end of the conical block (203) is fixedly connected with a limiting plate (204), one side of the limiting plate (204) is fixedly connected with a limiting spring (205) and is located at one end of the conical block (203).

3. The immune cell culture device of claim 2, wherein: The isolation mechanism (3) comprises a plurality of support plates (301), the plurality of support plates (301) are fixedly connected to the inner wall of the incubator body (1) and are located between the sealing door (101) and the telescopic column (201), one end of the support plate (301) is fixedly connected with an isolation plate (302) and is rotatably connected to the inner wall of the incubator body (1), one end of the support plate (301) close to the isolation plate (302) is fixedly installed with a rebounder (303) and penetrates into the inside of the support plate (301), one end of the rebounder (303) penetrating into the inside of the connecting column (304) is slidably connected with a connecting column (304), the top end of the support plate (301) is slidably connected with a tray (305), the bottom end of the tray (305) is fixedly connected with a sliding block (306) and penetrates into the inside of the support plate (301), the outer wall of the sliding block (306) is fixedly connected with a damping block (307) on both sides, one end of the sliding block (306) away from the rebounder (303) is fixedly connected with a supporting spring (308) and is located in the inside of the support plate (301), the bottom end of the connecting column (304) is fixedly connected with a C-shaped block (309) and penetrates into the sliding block (306) from one end of the connecting column (304), the bottom end of the C-shaped block (309) is fixedly connected with a reset spring (310).

4. The immune cell culture device of claim 2, wherein: The inside of the incubator body (1) is provided with a connecting groove matched with the telescopic column (201), the sealing door (101) is rotatably connected with the incubator body (1) through a hinge, one side of the telescopic column (201) is provided with a conical groove matched with the conical block (203).

5. The immune cell culture device of claim 3, wherein: The isolation plate (302) is provided with a fixed groove matched with the conical block (203) on one side close to the conical block (203), the isolation plate (302) is rotatably connected with the incubator main body (1) through a hinge, and the top end of the supporting plate (301) is provided with a sliding groove matched with the sliding block (306), and the two sides of the sliding groove are provided with damping grooves matched with the damping blocks (307).

6. The immune cell culture device of claim 3, wherein: The inside of the supporting plate (301) is provided with a supporting groove matched with the C-shaped block (309), the inside of the connecting column (304) is provided with a limiting groove matched with the C-shaped block (309), and the limiting groove in the connecting column (304) and the top end contact surface of the C-shaped block (309) are both provided as arc surfaces.

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