Facultative anaerobic fermentation device

Abstract

The facultative anaerobic fermentation device comprises a fermentation bottle, a rubber plug, an exhaust pipe and a fermentation bolt, the rubber plug is detachably connected with a bottle opening of the fermentation bottle, an exhaust port penetrating through the upper surface and the lower surface of the rubber plug is formed in the rubber plug, one end of the exhaust pipe penetrates through the exhaust port to be arranged in the fermentation bottle, and the other end of the exhaust pipe is connected with the fermentation bolt in a sealed mode. A sampling opening penetrating through the inner side wall and the outer side wall of the fermentation bottle is formed in the side wall, close to the bottle bottom, of the fermentation bottle, one end of the sampling pipe is a sample outlet with a downward opening, the other end of the sampling pipe is communicated with the sampling opening, a switch is arranged on the sampling pipe, when the switch is closed, the sampling pipe is kept sealed, and when the switch is opened, the sampling pipe is unsealed. The sampling pipe with the downward opening and the matched piston switch further enhance the sealing performance, ensure that external air or other pollutants are not introduced in the sampling process, allow a user to perform sampling operation under the condition that the fermentation environment is not interfered, and ensure the safety and stability of the fermentation process.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of facultative anaerobic fermentation device, especially to facultative anaerobic fermentation device. BACKGROUND

[0002] The microorganism that can grow and breed in aerobic or anaerobic environment is called facultative anaerobe, and the facultative anaerobic fermentation refers to the fermentation using facultative anaerobe. The researchers who study facultative anaerobe usually need to use specific fermentation device to ferment facultative anaerobe, so as to obtain the growth state and metabolic condition of the strain under different conditions. At present, the researchers usually use special fermentation equipment to carry out facultative anaerobic fermentation, but the special fermentation equipment is expensive, so the simple fermentation device used in the laboratory includes basic components such as fermentation bottle, rubber plug, exhaust pipe and fermentation plug. The rubber plug is detachably connected with the bottle opening of the fermentation bottle, the rubber plug is provided with an exhaust port penetrating the upper surface and the lower surface, one end of the exhaust pipe is arranged in the fermentation bottle through the exhaust port, and the other end is sealingly connected with the fermentation plug. The simple fermentation device is low in price and easy to operate, but usually has some shortcomings. For example, the fermentation plug and the fermentation bottle are combined to realize facultative anaerobic fermentation, but the device cannot take samples in the process, and usually needs to open the rubber plug at the bottle opening to take samples. However, the fermentation plug contains concentrated sulfuric acid in the experiment process, and opening the rubber plug is very dangerous because the concentrated sulfuric acid is easy to spill. At the same time, the sampling process contacts air, which can destroy the fermentation environment and thus cannot monitor the growth state and metabolic condition of the bacteria in different periods. How to improve the simple fermentation device to take samples in the process of facultative anaerobic fermentation becomes a difficult problem. CONTENT OF THE UTILITY MODEL

[0003] The technical problem to be solved by the utility model is to provide a facultative anaerobic fermentation device to solve the problem of sampling in the process of facultative anaerobic fermentation.

[0004] The utility model adopts the technical scheme that:

[0005] The facultative anaerobic fermentation device comprises a fermentation bottle, a rubber plug, an exhaust pipe and a fermentation plug. The rubber plug is detachably connected with the bottle opening of the fermentation bottle. The rubber plug is provided with an exhaust port penetrating the upper surface and the lower surface. One end of the exhaust pipe is arranged in the fermentation bottle through the exhaust port, and the other end is sealingly connected with the fermentation plug. The device comprises a sampling pipe. A sampling port penetrating the inner side wall and the outer side wall of the fermentation bottle is formed in the side wall of the fermentation bottle close to the bottle bottom. One end of the sampling pipe is provided with a sample outlet facing downward, and the other end is in communication with the sampling port. A switch is arranged on the sampling pipe. When the switch is closed, the sampling pipe is kept sealed. When the switch is opened, the sampling pipe is unsealed.

[0006] Further, a bellows type device is arranged on the exhaust pipe.

[0007] Further, the switch is a piston, and a through hole is formed in one side wall of the sampling tube, and the switch is in interference fit with the through hole.

[0008] Further, the through hole is upwardly open.

[0009] Further, the sampling tube is a glass straight tube, and an angle between a central axis of the sampling tube and a horizontal plane is not less than 10 degrees.

[0010] Further, the bottom of the fermentation bottle is provided with a cushion.

[0011] Further, the bottle body of the fermentation bottle is provided with a scale.

[0012] The beneficial effects of the present application are as follows:

[0013] The side wall close to the bottom of the fermentation bottle is provided with a sampling port penetrating the inner side wall and the outer side wall of the fermentation bottle, one end of the exhaust pipe is a sample outlet with an opening downward, the other end is in communication with the sampling port, and the sampling tube is provided with a switch, so that the user can perform sampling operation without opening the fermentation bottle, and the risk of concentrated sulfuric acid spilling is avoided. The downwardly open sampling tube and the piston switch further enhance the sealing performance, ensure that external air or other pollutants are not introduced during the sampling process, allow the user to perform sampling operation without disturbing the fermentation environment, and ensure the safety and stability of the fermentation process. Especially when the laboratory needs to frequently monitor the fermentation progress, this design is particularly important.

[0014] The bellows type device arranged on the exhaust pipe can prevent back suction and maintain the high sterility of the fermentation environment.

[0015] The through hole of the sampling tube is upwardly open, which makes the operation of the operator more convenient. The possibility of misoperation is reduced, and the convenience of use is further improved.

[0016] The bottle body of the fermentation bottle is provided with a scale, which facilitates the user to accurately measure the volume change of the fermentation liquid. This is of great significance for accurately controlling the fermentation conditions, adjusting the amount of reagent addition, etc., and helps to improve the quality of the fermentation sample. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments.

[0018] Figure 1 is a structure schematic view of a facultative anaerobic fermentation device.

[0019] In the figure, 1 is a fermentation bottle, 2 is a rubber plug, 3 is a fermentation plug, 4 is a sampling tube, and 5 is a switch. DETAILED DESCRIPTION

[0020] The present application will be further described below in combination with the drawings.

[0021] To make the objectives, technical solutions, and advantages of this utility model clearer, the following description, in conjunction with the accompanying drawings and embodiments, will provide a more detailed explanation of this utility model. It should be understood that the specific embodiments described below are only used to explain this utility model and are not intended to limit this utility model.

[0022] In the description of this utility model, it should be understood that the terms "upper surface", "lower surface", "one side", "opening downward", etc., indicating the orientation or positional relationship should be interpreted broadly and are not used to limit the protection scope of this utility model. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0023] like Figure 1 As shown, the facultative anaerobic fermentation device includes a fermentation bottle 1, a rubber stopper 2, an exhaust pipe, and a fermentation plug 3. The rubber stopper 2 is detachably connected to the mouth of the fermentation bottle 1. The rubber stopper 2 has an exhaust port that penetrates the upper and lower surfaces. One end of the exhaust pipe passes through the exhaust port and is placed inside the fermentation bottle 1, while the other end is sealed to the fermentation plug 3. The device also includes a sampling tube 4. A sampling port that penetrates the inner and outer walls of the fermentation bottle 1 is opened on the side wall near the bottom of the bottle. One end of the sampling tube 4 is a sample outlet with its opening facing downwards, and the other end is connected to the sampling port. The sampling tube 4 is equipped with a switch 5. When the switch 5 is closed, the sampling tube 4 remains sealed. When the switch 5 is opened, the sampling tube 4 is unsealed.

[0024] When conducting facultative anaerobic experiments, first check the sealing of sampling tube 4, ensuring that switch 5 is in the closed position. Add culture medium and microorganisms to fermentation bottle 1, connect fermentation plug 3 to fermentation bottle 1, add concentrated sulfuric acid or other reagents to fermentation plug 3 and seal it. Initially, the microorganisms utilize the oxygen in fermentation bottle 1 for aerobic fermentation. After the oxygen in fermentation bottle 1 is consumed, anaerobic fermentation begins. Gases such as carbon dioxide produced during fermentation are discharged through the exhaust pipe. Samples can be taken through the sampling port during fermentation. Air cannot enter fermentation bottle 1. After sampling, turn off switch 5 and continue the experiment. Sampling during the experiment is safe, pollution-free, and inexpensive.

[0025] Furthermore, the exhaust pipe is equipped with a plenum-type device.

[0026] Carbon dioxide gas is produced during fermentation, and this gas needs to be safely released to prevent excessive pressure inside the fermentation vessel from causing the bottle to rupture or other safety hazards. A belly-type device ensures that the gas can be smoothly released during fermentation, preventing backflow of concentrated sulfuric acid.

[0027] Furthermore, switch 5 is a piston, and a through hole is opened on one side wall of sampling tube 4. Switch 5 is interference-fitted with the through hole.

[0028] Switch 5 can also be selected as a pneumatic lock or a valve, but considering cost savings and reducing device complexity, this solution prefers to use a piston.

[0029] Furthermore, the through hole opening faces upwards.

[0030] The through-hole on the exhaust pipe faces upwards for easy operation.

[0031] Furthermore, sampling tube 4 is a straight glass tube, and the angle between the central axis of sampling tube 4 and the horizontal plane is not less than 10 degrees. This design further improves the sealing performance of the device.

[0032] Furthermore, a cushion is provided at the bottom of the fermentation bottle 1. When the sampling tube 4 is a straight glass tube and the angle between the central axis of the sampling tube 4 and the horizontal plane is not less than 10 degrees, a cushion is provided at the bottom of the fermentation bottle 1 to prevent the device from being unstable.

[0033] Furthermore, fermentation flask 1 is equipped with graduations. This allows operators to accurately measure the volume change of the fermentation broth. This is crucial for precisely controlling fermentation conditions and adjusting reagent dosages, thus contributing to improved sample quality.

[0034] The facultative anaerobic fermentation device provided by this utility model has no limitations on its preparation process and treatment method. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A facultative anaerobic fermentation apparatus, comprising a fermentation bottle (1), a rubber stopper (2), an exhaust pipe, and a fermentation plug (3), wherein the rubber stopper (2) is detachably connected to the mouth of the fermentation bottle (1), the rubber stopper (2) has an exhaust port penetrating the upper and lower surfaces, one end of the exhaust pipe passes through the exhaust port and is placed inside the fermentation bottle (1), and the other end is sealed to the fermentation plug (3), characterized in that, The sample tube (4) is included. A sampling port is opened on one side wall of the fermentation bottle (1) near the bottom of the bottle, which runs through the inner and outer walls of the fermentation bottle (1). One end of the sampling tube (4) is the sample outlet with the opening facing downwards, and the other end is connected to the sampling port. A switch (5) is provided on the sampling tube (4). When the switch (5) is closed, the inside of the sampling tube (4) remains sealed. When the switch (5) is opened, the inside of the sampling tube (4) is unsealed.

2. The facultative anaerobic fermentation apparatus according to claim 1, characterized in that, The exhaust pipe is equipped with a ventilated device.

3. The facultative anaerobic fermentation apparatus according to claim 1, characterized in that, The switch (5) is a piston, and a through hole is opened on the side wall of the sampling tube (4). The switch (5) is interference-fitted with the through hole.

4. The facultative anaerobic fermentation apparatus according to claim 3, characterized in that, The through hole faces upwards.

5. The facultative anaerobic fermentation apparatus according to claim 1, characterized in that, The sampling tube (4) is a straight glass tube, and the angle between the central axis of the sampling tube (4) and the horizontal plane is not less than 10 degrees.

6. The facultative anaerobic fermentation apparatus according to claim 5, characterized in that, The bottom of the fermentation bottle (1) is provided with a cushion.

7. The facultative anaerobic fermentation apparatus according to claim 1, characterized in that, The fermentation bottle (1) has graduations on its body.

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