Efficient preparation device for exosome

Abstract

The utility model relates to the technical field of exosome preparation, in particular to an efficient preparation device for exosomes, which comprises a tank body, a cover plate is covered at the upper end of the tank body, a net cylinder is arranged in the tank body, a central shaft is arranged at the bottom of the tank body, the lower end of the central shaft is fixedly assembled with the bottom of the tank body through a screw, and a shaft seat is arranged at the lower end of the net cylinder. When centrifugal filtration is needed, the filter cartridge is firstly put into the net cartridge, the filter cartridge forms a stable frame filter element structure through the combination of the two annular plates and the connecting rods, the stable support of the filter cartridge can be realized through the contact of the annular plate II and the support block, the assembly and disassembly of the filter cartridge and the net cartridge can be realized through the butt joint of the elastic clamp and the clamping groove, and then the centrifugal filtration is carried out. The mesh cylinder and the sealing cover are in butt joint, the mesh cylinder and the sealing cover can be fixed through mutual threaded butt joint of the external threads and the internal threads, and then exosome preparation substances needing centrifugal filtration can be injected into the filter cylinder through the material nozzle.

Description

Technical Field

[0001] This utility model relates to the field of exosome preparation technology, and in particular to a high-efficiency preparation device for exosomes. Background Technology

[0002] The preparation of exosomes is a complex process involving the isolation and purification of exosomes from cell culture medium or biological fluids such as blood, urine, saliva, etc.

[0003] As disclosed in Chinese Patent Publication No. CN221016640U, this invention provides a highly efficient device for preparing exosomes, comprising a separation chamber, a connecting cylinder at its center, a separation chamber installed inside the connecting cylinder, a preparation tank inside the separation chamber, a clamping mechanism for the preparation tank inside the separation cylinder, a connecting box fixedly connected to the bottom of the connecting cylinder, a connecting column fixedly connected to the bottom of the connecting cylinder, a first sliding column sleeved inside the connecting column, the first sliding column being fitted inside the connecting cylinder, a sliding mechanism for sliding the first sliding column inside the connecting box, a trough at the bottom of the preparation tank, multiple leak holes at the bottom of the trough, the multiple leak holes being evenly distributed in a ring, and a sludge discharge mechanism at the bottom of the preparation tank. This invention, through the setting of multiple limiting columns, provides greater stability during exosome preparation and facilitates the discharge of precipitates, thereby improving the effectiveness of the device.

[0004] Currently, when preparing exosomes, it is necessary to separate them. The most common method is centrifugation and filtration. However, the filter screen of the conventional centrifuge is not easy to disassemble, which makes cleaning and disinfection after each use quite cumbersome. In addition, after centrifugation, the exosome preparation material is very easy to adhere to the storage container, which is also relatively troublesome to clean and collect. Utility Model Content

[0005] The purpose of this invention is to address the aforementioned shortcomings in the existing technology by proposing a highly efficient device for the preparation of exosomes.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] Design an efficient preparation device for exosomes, including a tank, the upper end of which is covered with a cover plate, a mesh cylinder is arranged inside the tank, a central shaft is arranged at the bottom of the tank, the lower end of the central shaft is fixedly assembled to the bottom of the tank by screws, a shaft seat is arranged at the lower end of the mesh cylinder, the upper end of the shaft seat is welded and fixed to the bottom of the mesh cylinder, and the interior of the shaft seat is rotatably sleeved with the surface of the central shaft.

[0008] A bearing is installed through the center of the cover plate. The bearing is fixed to the cover plate by welding. A drive shaft is installed through the inside of the bearing. The outer wall of the drive shaft is interference-fitted with the inner wall of the bearing. A cover is installed at the lower end of the drive shaft. The cover is sleeved with the end of the mesh cylinder. The upper end of the cover is fixed to the lower end of the drive shaft by screws.

[0009] A motor is installed above the cover plate, and a drive shaft is installed inside the motor. The end of the drive shaft is fixedly connected to the drive shaft via a coupling.

[0010] A feeding nozzle is provided through one end of the cap, and the connection between the feeding nozzle and the cap is fixed by screws.

[0011] The inner side of the cover is provided with an internal thread, and the outer wall of the mesh cylinder is provided with an external thread, with the external thread and the internal thread being threadedly connected to each other.

[0012] In detail, the upper edge of the tank body is provided with threaded posts, the lower end of the threaded posts is welded and fixed to the end face of the tank body, the surface of the cover plate is provided with an inner sleeve, the connection position between the inner sleeve and the cover plate is fixed by welding, and the threaded posts and the inner sleeve are slidably sleeved together.

[0013] In detail, the end of the threaded column is threadedly connected to a threaded sleeve, and the end face of the threaded sleeve is tightly fitted to the end face of the inner sleeve.

[0014] In detail, the inside of the mesh cylinder is provided with a slidingly sleeved annular plate one and annular plate two, and a number of connecting rods are provided between annular plate one and annular plate two. The two ends of the connecting rods are fixed to the surface of annular plate one and the surface of annular plate two respectively by screws.

[0015] In detail, a filter cartridge is bonded to the upper end face of the second annular plate, and the upper open end face of the filter cartridge is bonded and fixed to the lower end face of the first annular plate. The filter cartridge is made of polypropylene material.

[0016] In detail, several support blocks are fixed to the lower end of the inner wall of the filter cylinder, and the upper end of the support blocks is closely attached to the lower end face of the filter cylinder.

[0017] In detail, the support block has slots inside, and the lower end of the filter cartridge is fixed with elastic clips of the same number as the slots, and the elastic clips and slots are interlocked.

[0018] In detail, the elastic card and the groove are both hexagonal in shape, and the elastic card is made of rubber material.

[0019] The design scheme proposed in this utility model has the following beneficial effects in application:

[0020] 1. When centrifugal filtration is required, first place the filter cartridge into the mesh cylinder. The filter cartridge forms a stable frame filter element structure through the combination of two annular plates and connecting rods. The filter cartridge is stably supported by the contact between the two annular plates and the support block. The filter cartridge and the mesh cylinder can be assembled and disassembled by the elastic clip and the slot. Then, the mesh cylinder is connected to the cover. The mesh cylinder and the cover can be fixed by the mutual threading of the external and internal threads. Then, the exosome preparation material to be centrifuged and filtered can be injected into the filter cartridge through the feed nozzle.

[0021] 2. The rotation of the motor causes the rotating shaft to drive the drive shaft to rotate, thereby enabling the cover and the screen cylinder to rotate. This allows the filter cylinder to perform centrifugal filtration. The rotation of the shaft seat on the central shaft ensures the stability of the lower end of the screen cylinder during rotation. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0023] Figure 2 This is a schematic diagram of the internal structure of the present invention;

[0024] Figure 3 This is a front cross-sectional view of the present invention;

[0025] Figure 4 This is an enlarged schematic diagram of point A of this utility model.

[0026] In the diagram: 1. Tank body; 11. Cover plate; 12. Central shaft; 13. Shaft seat; 14. Cover; 15. Mesh cylinder; 16. Bearing; 17. Drive shaft; 18. Motor; 19. Nozzle; 110. Internal thread; 111. External thread; 2. Internal sleeve; 21. Threaded column; 22. Threaded sleeve; 3. Annular plate one; 31. Annular plate two; 32. Connecting rod; 33. Filter cartridge; 3001. Support block; 3002. Slot; 3003. Elastic clip. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0028] Reference Figures 1-4A highly efficient preparation device for exosomes includes a tank 1, the upper end of which is covered by a cover plate 11, a mesh cylinder 15 is provided inside the tank 1, a central shaft 12 is provided at the bottom of the tank 1, the lower end of the central shaft 12 is fixedly assembled to the bottom of the tank 1 by screws, a shaft seat 13 is provided at the lower end of the mesh cylinder 15, the upper end of the shaft seat 13 is welded and fixed to the bottom of the mesh cylinder 15, and the interior of the shaft seat 13 is rotatably sleeved with the surface of the central shaft 12.

[0029] A bearing 16 is installed through the center of the cover plate 11. The bearing 16 is fixed to the cover plate 11 by welding. A drive shaft 17 is installed through the inside of the bearing 16. The outer wall of the drive shaft 17 is press-fitted with the inner wall of the bearing 16. A cover 14 is installed at the lower end of the drive shaft 17. The cover 14 is sleeved with the end of the mesh cylinder 15. The upper end of the cover 14 is fixed to the lower end of the drive shaft 17 by screws.

[0030] A motor 18 is provided above the cover plate 11. The motor 18 has a drive shaft inside. The end of the drive shaft is fixedly connected to the drive shaft 17 via a coupling.

[0031] A feed nozzle 19 is provided through one end of the cover 14, and the connection between the feed nozzle 19 and the cover 14 is fixed by screws.

[0032] The inner side of the cover 14 is provided with an internal thread 110, and the outer wall of the mesh cylinder 15 is provided with an external thread 111. The external thread 111 and the internal thread 110 are threaded together.

[0033] It should be further explained that threaded posts 21 are distributed on the upper edge of the tank body 1, and the lower end of the threaded posts 21 is welded and fixed to the end face of the tank body 1. An inner sleeve 2 is provided through the surface of the cover plate 11, and the connection position between the inner sleeve 2 and the cover plate 11 is fixed by welding. The threaded posts 21 and the inner sleeve 2 are slidably sleeved together.

[0034] It should be further noted that the threaded end of the threaded column 21 is threadedly connected to a threaded sleeve 22, and the end face of the threaded sleeve 22 is tightly fitted to the end face of the inner sleeve 2.

[0035] It should be further explained that the inside of the screen cylinder 15 is provided with a sliding sleeve of annular plate 3 and annular plate 31. Several connecting rods 32 are provided between annular plate 3 and annular plate 31. The two ends of the connecting rods 32 are fixed to the surface of annular plate 3 and annular plate 31 respectively by screws. This facilitates the assembly and disassembly of the structure of the filter part with the tank 1, so that the filtered material can be easily recycled after filtration.

[0036] It should be further noted that a filter cartridge 33 is bonded to the upper end face of the second annular plate 31. The upper open end face of the filter cartridge 33 is bonded and fixed to the lower end face of the first annular plate 3. The filter cartridge 33 is made of polypropylene material. When the exosome preparation material is centrifuged, the filter cartridge 33 is usually centrifuged at a centrifugal force of 300-500g for 10 minutes to remove cells and larger cell debris.

[0037] It should be further noted that several support blocks 3001 are fixed at the lower end of the inner wall of the mesh cylinder 15, and the upper end of the support block 3001 is closely attached to the lower end face of the filter cylinder 33.

[0038] It should be further noted that the support block 3001 has a slot 3002 inside, and the lower end of the filter cylinder 33 is fixed with the same number of elastic clips 3003 as the slot 3002. The elastic clips 3003 and the slot 3002 are interlocked, which facilitates the assembly and disassembly of the filter cylinder 33 and the mesh cylinder 15.

[0039] It should be further noted that the shape of the elastic card 3003 and the inner shape of the slot 3002 are both regular hexagons. The elastic card 3003 is made of rubber material, which can ensure the stability of the engagement between the elastic card 3003 and the slot 3002 and the ease of disassembly.

[0040] Working method: When centrifugal filtration is required, the filter cartridge 33 is first placed into the mesh cylinder 15. The filter cartridge 33 forms a stable frame filter element structure through the combination of two annular plates and connecting rod 32. The filter cartridge 33 can be stably supported by the contact between the second annular plate 31 and the support block 3001. The filter cartridge 33 and the mesh cylinder 15 can be assembled and disassembled by the docking of the elastic clip 3003 and the slot 3002. Then, the mesh cylinder 15 is docked with the cover 14. The mesh cylinder 15 and the cover 14 can be fixed by the mutual threading of the external thread 111 and the internal thread 110. Then, the exosome preparation material to be centrifuged and filtered can be injected into the filter cartridge 33 through the feed nozzle 19.

[0041] The rotation of motor 18 causes the rotating shaft to drive the drive shaft 17 to rotate, thereby enabling the rotation of the cap 14 and the screen cylinder 15. This allows the filter cylinder 33 to perform centrifugal filtration. The rotation of the bearing seat 13 on the central shaft 12 ensures the stability of the lower end of the screen cylinder 15 during rotation, allowing the exosome preparation material filtered out by the filter cylinder 33 to be filtered out a second time through the screen cylinder 15 and collected inside the tank 1.

[0042] When the cover plate 11 needs to be opened, rotate the threaded sleeve 22 so that the threaded sleeve 22 and the threaded post 21 are separated by threads, which can realize the disengagement operation of the inner sleeve 2 and the threaded post 21. Thus, the tank body 1 and the cover plate 11 can be separated, so that the exosome preparation material that has been centrifuged and filtered into the tank body 1 can be poured out.

[0043] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A highly efficient apparatus for preparing exosomes, comprising a container (1), characterized in that: The upper end of the tank (1) is covered with a cover plate (11), a mesh cylinder (15) is provided inside the tank (1), a central shaft (12) is provided at the bottom of the tank (1), the lower end of the central shaft (12) is fixedly assembled with the bottom of the tank (1) by screws, a shaft seat (13) is provided at the lower end of the mesh cylinder (15), the upper end of the shaft seat (13) is welded and fixed to the bottom of the mesh cylinder (15), and the interior of the shaft seat (13) is rotatably sleeved with the surface of the central shaft (12); A bearing (16) is provided through the center of the cover plate (11). The bearing (16) and the cover plate (11) are fixed by welding. A drive shaft (17) is provided through the inside of the bearing (16). The outer wall of the drive shaft (17) is press-fitted with the inner surface of the bearing (16). A cover (14) is provided at the lower end of the drive shaft (17). The cover (14) is sleeved with the end of the mesh cylinder (15). The upper end of the cover (14) is fixed with the lower end of the drive shaft (17) by screws. A motor (18) is provided above the cover plate (11). The motor (18) has a drive shaft inside. The end of the drive shaft is fixedly connected to the drive shaft (17) through a coupling. A feed nozzle (19) is provided through one end of the cover (14), and the connection between the feed nozzle (19) and the cover (14) is fixedly assembled by screws; The inner side of the cover (14) is provided with an internal thread (110), and the outer wall of the mesh cylinder (15) is provided with an external thread (111). The external thread (111) and the internal thread (110) are threaded together.

2. The efficient apparatus for preparing exosomes according to claim 1, characterized in that: The upper edge of the tank (1) is provided with threaded columns (21), the lower end of the threaded columns (21) is welded and fixed to the end face of the tank (1), and an inner sleeve (2) is provided through the surface of the cover plate (11). The connection position between the inner sleeve (2) and the cover plate (11) is fixed by welding, and the threaded columns (21) and the inner sleeve (2) are slidably sleeved together.

3. The efficient apparatus for preparing exosomes according to claim 2, characterized in that: The threaded column (21) has a threaded sleeve (22) at its end, and the end face of the threaded sleeve (22) is closely attached to the end face of the inner sleeve (2).

4. The efficient apparatus for preparing exosomes according to claim 1, characterized in that: The inside of the mesh cylinder (15) is provided with a sliding sleeve of an annular plate one (3) and an annular plate two (31). A number of connecting rods (32) are provided between the annular plate one (3) and the annular plate two (31). The two ends of the connecting rods (32) are fixed to the surface of the annular plate one (3) and the surface of the annular plate two (31) respectively by screws.

5. The efficient apparatus for preparing exosomes according to claim 4, characterized in that: A filter cartridge (33) is bonded to the upper end face of the second annular plate (31). The upper open end face of the filter cartridge (33) is bonded and fixed to the lower end face of the first annular plate (3). The filter cartridge (33) is made of polypropylene material.

6. The efficient apparatus for preparing exosomes according to claim 5, characterized in that: The lower end of the inner wall of the mesh cylinder (15) is fixed with several support blocks (3001), and the upper end of the support blocks (3001) is closely attached to the lower end face of the filter cylinder (33).

7. The efficient apparatus for preparing exosomes according to claim 6, characterized in that: The support block (3001) has a slot (3002) inside, and the lower end of the filter cartridge (33) is fixed with an elastic clip (3003) in the same number as the slot (3002). The elastic clip (3003) and the slot (3002) are interlocked.

8. The efficient apparatus for preparing exosomes according to claim 7, characterized in that: The elastic card (3003) and the groove of the card slot (3002) are both regular hexagonal, and the elastic card (3003) is made of rubber material.

Images