Cellulose enzymolysis device for corn seeds
By utilizing a combination of a stirring rod and a dosing tube in the cellulose enzymatic hydrolysis device for corn seeds, uniform pH adjustment was achieved, solving the problem of uneven pH in the enzymatic hydrolysis reaction and improving the hydrolysis efficiency.
Patent Information
- Application Number
- CN202520162301.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-24
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2035-01-24
AI Technical Summary
In existing technologies, uneven pH adjustment during enzymatic hydrolysis leads to inconsistent reaction efficiency, thus affecting the overall efficiency of the enzymatic hydrolysis reaction.
A cellulosic hydrolysis device for corn seeds is used, including a reaction vessel, a stirring rod, a dosing tube, and a dosing assembly. The position of the dosing hole is moved by the rotation of the stirring rod to uniformly spray the pH adjusting liquid. Combined with the baffle and agitation mechanism, the uniformity of pH adjustment and the efficiency of the enzymatic hydrolysis reaction are ensured.
This achieves uniform pH adjustment, improves the efficiency and consistency of the enzymatic hydrolysis reaction, and ensures the smooth progress of the enzymatic hydrolysis process.
Smart Images
Figure CN223837433U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of enzymatic hydrolysis reactions, and in particular to an enzymatic hydrolysis device for corn seeds. Background Technology
[0002] Currently, cellulase has wide applications in agriculture and industry, especially in the processing of corn seeds. Enzymatic hydrolysis can release starch and protein trapped inside the fiber, thereby improving the utilization rate of these resources.
[0003] In existing technologies, corn seeds are typically crushed before enzymatic hydrolysis. Cellulase is then added to the enzymatic hydrolysis tank, and the pH value is adjusted. Alternatively, the pH value can be adjusted first, followed by the addition of cellulase. However, pH adjustment is usually done by adding an additive. When the additive does not contact the original solution evenly, the reaction efficiency at different locations will be inconsistent, resulting in inaccurate pH adjustment and affecting the efficiency of the enzymatic hydrolysis reaction. Utility Model Content
[0004] To reduce the impact on the efficiency of the enzymatic hydrolysis reaction, this application provides a cellulose enzymatic hydrolysis device for corn seeds.
[0005] This application provides a cellulose enzymatic hydrolysis device for corn seeds, which adopts the following technical solution:
[0006] A cellulose enzymatic hydrolysis device for corn seeds, comprising:
[0007] The reaction vessel has a reaction chamber, a feeding port, and a detachable cap for filling raw materials.
[0008] The temperature control component is located on the inner wall of the reaction vessel and is used to control the temperature inside the reaction chamber.
[0009] A stirring rod rotates on the reaction vessel and is located inside the reaction chamber;
[0010] A drive assembly is disposed on the reaction vessel and connected to the stirring rod;
[0011] An additive tube is mounted on the stirring rod and has an additive hole.
[0012] A reagent supply assembly is disposed on the reaction vessel or on one side of the reaction vessel and connected to the reagent addition pipe for supplying a liquid reagent for pH adjustment.
[0013] By adopting the above technical solution, firstly, the user needs to prepare corn seeds as raw materials, then crush and sieve them to form corn seed granules; next, open the lid on the reaction tank and fill the reaction chamber of the reaction tank through the feeding hole; after filling, close the lid again; before the reaction starts, the temperature in the reaction chamber is preset and adjusted by the temperature control component to ensure that the enzymatic hydrolysis reaction is carried out at a suitable temperature; start the drive component to drive the stirring rod to rotate in the reaction chamber; the stirring rod not only helps to mix the raw materials evenly, but also promotes the enzymatic hydrolysis reaction; during the enzymatic hydrolysis process, cellulase acts on the cellulose components of the corn seeds, breaking them down into smaller molecules.
[0014] During enzymatic hydrolysis, a pH-adjusting liquid may need to be added to the reaction chamber through the dosing tube to maintain a suitable acidity or alkalinity. The dosing assembly is responsible for delivering the liquid from the storage container to the dosing tube and spraying it into the reaction chamber through the dosing orifice. The position of the dosing orifice rotates with the position of the stirring rod, making the pH adjustment faster and more uniform, reducing local pH inconsistencies, and maintaining the efficiency of the cellulose enzymatic hydrolysis reaction. Users can adjust the amount and timing of the liquid supply as needed to ensure the smooth progress of the enzymatic hydrolysis reaction. Furthermore, the pH can be adjusted at any time during the reaction to maintain optimal reaction efficiency, and the reaction efficiency can also be controlled by adjusting the pH.
[0015] Optionally, the agent supply assembly includes:
[0016] A connecting ring is disposed on the stirring rod and located outside the reaction vessel, connected to the dosing pipe, and forming a liquid dosing chamber;
[0017] A sealing ring is disposed on the reaction vessel, rotatably and sealingly connected with the connecting ring, and has a liquid supply chamber communicating with the liquid addition chamber;
[0018] A liquid addition tank, located on one side of the reaction vessel, is used to store the pH adjustment liquid agent and is connected to the sealing ring via a power component.
[0019] By adopting the above technical solution, before starting the enzymatic hydrolysis reaction, ensure that the addition tank is filled with an appropriate amount of pH adjustment solution; the power component (such as a pump) is in standby mode, ready to transport the solution from the addition tank to the supply chamber; the connecting ring is fixed on the stirring rod and located outside the reaction vessel. The addition tube is connected to the connecting ring to form a closed addition chamber; the sealing ring is tightly fitted to the reaction vessel and rotates and seals with the connecting ring. In this way, even when the stirring rod rotates within the reaction chamber, the seal between the addition chamber and the supply chamber is maintained. The supply chamber and the addition chamber are connected by a channel on the sealing ring, ensuring that the liquid can be smoothly transported from the addition tank to the addition chamber and further into the reaction chamber. When it is necessary to adjust the pH within the reaction chamber, the power unit (such as a pump) is activated. The power unit transports the liquid from the addition tank to the supply chamber of the sealing ring through a pipeline. Due to the sealed connection between the connecting ring and the sealing ring, the liquid will not leak outside the reaction vessel. The liquid enters the addition chamber through the supply chamber and is evenly sprayed into the reaction chamber through the addition hole on the addition pipe. During the enzymatic hydrolysis reaction, the power unit is activated to supply the liquid as needed and based on monitoring results. By precisely controlling the amount and timing of the liquid supply, the pH within the reaction chamber can be maintained within a suitable range. Furthermore, the use of the above structure allows for pH adjustment during stirring.
[0020] Optionally, the stirring rod is rotatably equipped with baffles, which rotate and move closer to or further away from the additive orifice.
[0021] By adopting the above technical solution, during the addition process or during stirring, the raw materials in the reaction vessel are disturbed, causing the turbulence blades to rotate. Alternatively, the turbulence blades can be rotated under the impetus of the pH adjustment liquid sprayed from the addition orifice. By controlling the spray force, the rotation angle of the turbulence blades can be adjusted to increase the disturbance to the raw materials, thereby making the contact between the enzyme and cellulose more sufficient and facilitating the promotion of reaction efficiency.
[0022] Optionally, the deflector blades are tilted.
[0023] By adopting the above technical solution, the inclined turbulence blades increase the turbulence area of the stirring blades, thereby increasing the turbulence effect and further enhancing the contact between the enzyme and cellulose, which facilitates the promotion of reaction efficiency.
[0024] Optionally, the turbulence vanes can block the dosing orifice.
[0025] By adopting the above technical solution, when the dosing hole is not spraying the liquid agent for pH adjustment, the turbulence blades will block the dosing hole under their own gravity, reducing the amount of raw material entering the dosing hole and reducing the contamination of the dosing hole and dosing pipe.
[0026] Optionally, the sealing ring is provided with an air supply assembly, the air supply assembly comprising:
[0027] An air supply ring is disposed on the sealing ring, forming an air storage chamber, and has an air filling hole communicating with the liquid supply chamber;
[0028] A gas supply unit, installed on the reaction vessel and connected to the gas supply ring, is used to supply oxygen or other inert gases.
[0029] By adopting the above technical solution, during the reaction process, oxygen is supplied to the reaction vessel through the gas supply device and the dosing pipe to create a good enzymatic hydrolysis environment and promote enzymatic hydrolysis efficiency. At the same time, the injection of pH adjustment liquid can be pressurized, and when pH adjustment liquid is not added, oxygen or other gases can be supplied to reduce the blockage of the dosing pipe and increase the disturbance to the raw materials in the reaction vessel.
[0030] Optionally, the reaction vessel is provided with a disturbance mechanism, the disturbance mechanism comprising:
[0031] The disturbance rod slides within the reaction vessel and moves vertically.
[0032] A disturbance leaf is disposed on the disturbance rod;
[0033] A sliding assembly is disposed inside the reaction vessel and connected to the disturbance rod, used to drive the disturbance rod to slide.
[0034] By adopting the above technical solution, during the enzymatic hydrolysis process, the sliding component drives the disturbance rod to slide, and the disturbance rod drives the disturbance blade to disturb the raw materials in the reaction vessel, thereby increasing the mixing effect and improving the enzymatic hydrolysis efficiency.
[0035] Optionally, the sliding component includes:
[0036] A drive ring, connected to the disturbance rod, has a drive groove.
[0037] The drive block is mounted on the stirring rod and located on the side of the drive ring near the bottom wall of the reaction vessel, and can slide into or out of the drive groove.
[0038] By adopting the above technical solution, as the stirring rod rotates, the stirring rod drives the drive block to abut against the drive ring and rotate. When the drive block enters the drive groove, the drive ring drives the disturbance rod and disturbance blade to sink. Then, as the drive block slides out of the drive groove, the drive ring moves upward, causing the disturbance blade to move upward in the raw material, thereby completing the disturbance of the raw material, increasing the contact between the enzyme and the corn seeds, and thus enhancing the enzymatic hydrolysis efficiency.
[0039] In summary, this application includes at least one of the following beneficial technical effects:
[0040] 1. During enzymatic hydrolysis, a pH-adjusting liquid may need to be added to the reaction chamber through the dosing tube to maintain a suitable pH level. The dosing assembly is responsible for delivering the liquid from the storage container to the dosing tube and spraying it into the reaction chamber through the dosing orifice. The position of the dosing orifice rotates with the position of the stirring rod, making pH adjustment faster and more uniform, reducing local pH inconsistencies, and maintaining the efficiency of the cellulose enzymatic hydrolysis reaction. Users can adjust the supply amount and timing of the liquid as needed to ensure the smooth progress of the enzymatic hydrolysis reaction. Furthermore, pH can be adjusted continuously during the reaction to maintain optimal reaction efficiency and control the reaction efficiency.
[0041] 2. As the stirring rod rotates, it drives the drive block to abut against the drive ring and rotate. When the drive block enters the drive groove, the drive ring drives the disturbance rod and disturbance blade to sink. Then, as the drive block slides out of the drive groove, the drive ring moves upward, causing the disturbance blade to move upward in the raw material, thereby disturbing the raw material, increasing the contact between the enzyme and the corn seeds, and thus enhancing the enzymatic hydrolysis efficiency. Attached Figure Description
[0042] Figure 1 This is a cross-sectional view of the enzymatic hydrolysis device in the embodiments of this application;
[0043] Figure 2 yes Figure 1 Enlarged view of A in the middle;
[0044] Figure 3 yes Figure 1 Enlarged view of B in the middle;
[0045] Figure 4 yes Figure 1 A magnified view of C.
[0046] Reference numerals: 100, reaction vessel; 110, reaction chamber; 200, stirring rod; 300, drive assembly; 400, dosing pipe; 410, dosing hole; 500, supply assembly; 510, connecting ring; 511, liquid filling chamber; 520, sealing ring; 521, liquid supply chamber; 530, liquid filling tank; 610, turbulence vane; 620, support; 700, gas supply assembly; 710, gas supply ring; 711, gas storage chamber; 712, gas filling hole; 720, gas supply component; 800, disturbance mechanism; 810, disturbance rod; 820, disturbance vane; 830, sliding assembly; 831, drive ring; 832, drive block; 833, drive groove. Detailed Implementation
[0047] The following combination Figures 1 to 4 This application will be described in further detail.
[0048] This embodiment discloses a cellulose enzymatic hydrolysis device for corn seeds.
[0049] Reference Figure 1 , Figure 2 and Figure 3 A cellulosic hydrolysis apparatus for corn seeds includes: a reaction vessel 100 for providing a reaction chamber 110; a cap detachably connected to the top wall of the reaction vessel 100; a temperature control component disposed on the reaction vessel 100; a stirring rod 200 rotatably disposed on the top wall of the reaction vessel 100; a drive assembly 300 disposed on the reaction vessel 100 and connected to the stirring rod 200; a dosing tube 400 disposed on the stirring rod 200 and rotating with the stirring rod 200; and a supply assembly 500 disposed on the reaction vessel 100 for supplying a pH-adjusting liquid agent to the dosing tube 400. In the enzymatic hydrolysis of cellulose, the corn seeds are first crushed, and then the crushed corn seed particles are screened using a sieve of a specified particle size. The screened corn seed particles are then added into the reaction tank 100 through a cap. Then, a pH adjusting agent is added into the reaction tank 100 through the agent supply component 500 and the agent addition tube 400 to adjust the pH value. During the agent addition process, the stirring rod 200 is rotated by the drive component 300. After the pH value is adjusted, cellulase is added, and then the temperature is adjusted by the temperature control component to carry out the enzymatic hydrolysis.
[0050] Cellulase is not a monomeric enzyme, but a multi-component enzyme system that works synergistically. It is a complex enzyme mainly composed of the following enzymes: exo-β-glucanase: acts on the ends of cellulose polysaccharide chains to release glucose or cellobiose; endo-β-glucanase: randomly cleaves the amorphous regions inside the cellulose polysaccharide chains to produce oligosaccharides of different lengths and the ends of new chains; β-glucosidase: hydrolyzes cellobiose to produce two molecules of glucose; in addition, the cellulase system also includes enzymes such as highly active xylanase.
[0051] The reaction vessel 100 is cylindrical and is placed on the ground by support legs. A feeding hole is provided on the upper top wall of the reaction vessel 100, and a cover is placed on the feeding hole and is detachably connected by bolts.
[0052] The temperature control component includes a coil installed on the inner wall of the reaction vessel 100, which can be filled with hot or cold water to heat or cool the reaction chamber 110. The stirring rod 200 includes a vertical pipe section and multiple diffusion sections perpendicular to the vertical pipe section. The diffusion sections and the vertical pipe section form a tree-like or fishbone-like shape. Multiple dosing pipes 400 are provided and correspond to the diffusion sections.
[0053] The drive assembly 300 includes a drive motor, the output shaft of which is connected to the stirring rod 200; the supply assembly 500 includes a connecting ring 510, which is fixedly connected to one end of multiple dosing tubes 400 near the drive motor and fixedly connected to the stirring rod 200. A liquid addition chamber 511 is provided inside the connecting ring 510, communicating with the dosing tubes 400. Multiple dosing holes 410 are provided on the dosing tubes 400, communicating with the reaction chamber 110 of the reaction vessel 100; a bracket 620 is fixedly connected to the reaction vessel 100, and a... A sealing ring 520 is attached, which is sleeved on the outside of the connecting ring 510 and is rotatably and sealingly connected to the connecting ring 510. A connecting hole is provided on the outer wall of the connecting ring 510, and a liquid supply chamber 521 is formed on the side wall of the sealing ring 520 near the connecting ring 510. The liquid supply chamber 521 is connected to the liquid addition chamber 511 through the connecting hole. A liquid addition tank 530 is provided on the reaction vessel 100 or on one side of the reaction vessel 100. The liquid addition tank 530 is connected to the sealing ring 520 through a hose and a pump. A storage chamber is formed inside the liquid addition tank 530, and the storage chamber is filled with a pH adjustment liquid.
[0054] A gas supply assembly 700 is provided on the sealing ring 520. The gas supply assembly 700 includes a gas supply ring 710 fixedly connected to the sealing ring 520. The gas supply ring 710 is located on the side of the sealing ring 520 away from the reaction vessel 100 and is coaxially connected. A gas storage chamber 711 is opened in the gas supply ring 710. A gas filling hole 712 is opened on the side wall of the gas supply ring 710 near the sealing ring 520. The gas storage chamber 711 is connected to the liquid supply chamber 521 through the gas filling hole 712. A gas supply component 720 is provided on the reaction vessel 100 or on one side of the reaction vessel 100. The gas supply component 720 can be a pressure vessel, a blower, or a fan. When oxygen supply or inert gas supply is required, it can be achieved by storing a specific gas in the pressure vessel. In this embodiment, a pressure vessel is preferred. The pressure vessel is filled with oxygen for supplying oxygen.
[0055] To facilitate the sealing of the dosing hole 410 on the dosing tube 400, a baffle 610 is rotatably connected to the stirring blade or the dosing tube 400. In this embodiment, the baffle 610 is preferably set on the dosing tube 400. The baffle 610 is set at an angle and is close to or away from the dosing hole 410. When there is no power blowing, it can cover the dosing hole 410 by its own weight. When the baffle 610 rotates with the stirring rod 200, it can disturb the corn seed particles.
[0056] A disturbance mechanism 800 is provided on the reaction vessel 100. The disturbance mechanism 800 includes a plurality of disturbance rods 810 that slide vertically within the reaction vessel 100. The plurality of disturbance rods 810 are equally spaced along the circumference of the reaction vessel 100. A disturbance leaf 820 is fixedly connected to one end of the disturbance rod 810 located in the corn seed kernel. The plurality of disturbance rods 810 are connected to a sliding assembly 830. The sliding assembly 830 is provided on the reaction vessel 100 and is used to drive the disturbance rods 810 to slide vertically.
[0057] Reference Figure 1 and Figure 4 The sliding assembly 830 includes a drive ring 831 sleeved on the outside of the stirring rod 200. The drive ring 831 can slide freely along the length of the stirring rod 200. The drive ring 831 is fixedly connected to one end of a plurality of disturbance rods 810 away from the disturbance blade 820. A plurality of drive grooves 833 are provided on the side wall of the drive ring 831 near the disturbance blade 820, and the drive grooves 833 penetrate the drive ring 831. A drive block 832 is fixedly connected to the stirring shaft. A plurality of drive blocks 832 can be provided. In this example, two are preferred. The two fixed blocks face the drive ring 831 and can abut against the side wall of the drive ring 831 near the disturbance blade 820. Their positions correspond to the drive grooves 833. As the drive blocks 832 rotate, they can slide into the drive grooves 833 and slide out of the drive grooves 833.
[0058] The implementation principle of the cellulose enzymatic hydrolysis device for corn seeds in this application embodiment is as follows: First, the corn seeds are crushed, and then the crushed corn seed particles are screened using a sieve of a specified particle size to ensure uniform particle size, which is beneficial to the subsequent enzymatic hydrolysis reaction. Next, the cover on the reaction tank 100 is opened, and the screened corn seed particles are added into the reaction tank 100 through the feeding hole. After the corn seed particles are added to the reaction tank 100, pH adjustment begins. The liquid tank 530 in the agent supply assembly 500 stores pH adjustment liquid, which is delivered to the supply chamber 521 in the sealing ring 520 through a pump and hose. Since the connecting ring 510 and the sealing ring 520 are rotatably sealed, and the agent supply pipe 400 is connected to the supply chamber 511 in the connecting ring 510, when the stirring rod 200 rotates, the agent supply pipe 400 also rotates, and the pH adjustment liquid is evenly sprayed onto the corn seed particles in the reaction tank 100 through the agent supply hole 410. During this process, the drive motor in the drive assembly 300 drives the stirring rod 200 to rotate, which helps to fully mix the liquid with the corn seed particles.
[0059] If oxygen is required for the enzymatic hydrolysis process, it can be supplied to the gas storage chamber 711 through the pressure tank in the gas supply assembly 700. As the stirring rod 200 rotates, the oxygen in the gas storage chamber 711 enters the liquid supply chamber 521 through the gas filling hole 712, and may further contact the corn seed particles, providing the necessary oxygen environment for the enzymatic hydrolysis reaction. After the pH value is adjusted, cellulase is added. At this time, the temperature control assembly starts working, heating or cooling the reaction vessel 100 with hot or cold water in the coil to maintain the suitable temperature required for the enzymatic hydrolysis reaction. The stirring rod 200 continues to rotate to ensure that the cellulase and corn seed particles are fully mixed and the enzymatic hydrolysis reaction occurs.
[0060] During enzymatic hydrolysis, the agitation mechanism 800 further promotes the mixing and agitation of the corn seed particles. As the stirring rod 200 rotates, the drive block 832 enters and slides out of the drive groove 833, driving the drive ring 831 and the multiple agitation rods 810 fixed thereto to slide vertically downwards. The agitation blades 820 at the ends of the agitation rods 810 agitate the corn seed particles, which helps to ensure the uniformity of the enzymatic hydrolysis reaction.
[0061] Furthermore, if the pH value changes during the enzymatic hydrolysis process, pH adjustment liquid can be added through the dosing tube 400 to adjust the pH value.
[0062] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.
Claims
1. A cellulose enzymatic hydrolysis device for corn seeds, characterized in that: include: The reaction vessel (100) has a reaction chamber (110), a feeding port, and a detachable cap for filling raw materials; A temperature control component is installed on the inner wall of the reaction vessel (100) to control the temperature inside the reaction chamber (110); A stirring rod (200) rotates on the reaction vessel (100) and is located inside the reaction chamber (110); A drive assembly (300) is disposed on the reaction vessel (100) and connected to the stirring rod (200); An additive tube (400) is disposed on the stirring rod (200) and has an additive hole (410). A supply assembly (500) is disposed on the reaction vessel (100) or on one side of the reaction vessel (100) and connected to the dosing pipe (400) for supplying a liquid agent for pH adjustment.
2. The enzymatic hydrolysis device for corn seeds according to claim 1, characterized in that: The agent supply assembly (500) includes: A connecting ring (510) is disposed on the stirring rod (200) and located outside the reaction vessel (100), connected to the dosing tube (400), and forming a liquid dosing chamber (511). A sealing ring (520) is disposed on the reaction vessel (100), and is rotatably and sealingly connected with the connecting ring (510), and has a liquid supply chamber (521) communicating with the liquid addition chamber (511). A liquid addition tank (530) is located on one side of the reaction vessel (100) for storing pH adjustment liquid and is connected to a sealing ring (520) via a power component.
3. The enzymatic hydrolysis device for corn seeds according to claim 2, characterized in that: The stirring rod (200) is rotatably equipped with a baffle (610), which rotates and moves closer to or further away from the additive hole (410).
4. The enzymatic hydrolysis device for corn seeds according to claim 3, characterized in that: The deflector (610) is inclined.
5. The enzymatic hydrolysis device for corn seeds according to claim 3 or 4, characterized in that: The turbulence vane (610) can block the additive orifice (410).
6. The enzymatic hydrolysis device for corn seeds according to any one of claims 2-4, characterized in that: An air supply assembly (700) is provided on the sealing ring (520), the air supply assembly (700) comprising: An air supply ring (710) is disposed on the sealing ring (520) to form an air storage chamber (711) and an air filling hole (712) communicating with the liquid supply chamber (521). A gas supply unit (720) is installed on the reaction vessel (100) and connected to the gas supply ring (710) for supplying oxygen or inert gas.
7. The enzymatic hydrolysis device for corn seeds according to claim 6, characterized in that: The reaction vessel (100) is equipped with a disturbance mechanism (800), which includes: The disturbance rod (810) slides within the reaction vessel (100) and slides vertically. A disturbance blade (820) is disposed on the disturbance rod (810); A sliding assembly (830) is disposed inside the reaction vessel (100) and connected to the disturbance rod (810) for driving the disturbance rod (810) to slide.
8. The enzymatic hydrolysis device for corn seeds according to claim 7, characterized in that: The sliding assembly (830) includes: The drive ring (831) is connected to the disturbance rod (810) and has a drive groove (833). The drive block (832) is disposed on the stirring rod (200) and located on the side of the drive ring (831) near the bottom wall of the reaction vessel (100), and can slide into or out of the drive groove (833).