Rapid dyeing and decolorizing device for albumen glue coomassie brilliant blue

Abstract

The utility model relates to the technical field of albumen glue coomassie brilliant blue processing, and discloses an albumen glue coomassie brilliant blue rapid dyeing and decoloring device which comprises a water bath box, the four supporting legs are fixedly connected to the bottom surface of the water bath box; the through groove penetrates through the front surface of the water bath box; the glass window is fixedly connected to the inner wall of the through groove; the inner wall of the box cover is in threaded connection with the inner wall of the water bath box through bolts; the dyeing and decoloring bottle is positioned in the water bath box; the top end surface of the dyeing and decoloring bottle penetrates through the top bottom surface of the box cover and the inner wall of the through hole and extends to the upper part of the box cover; and the sealing plug is positioned above the dyeing and decoloring bottle. Through the rotating assembly, the clamping plate is used for driving the rubber plate to clamp and fix the dyeing and decoloring bottle in the annular plate, and the motor is used for driving the supporting frame and the annular plate to rotate, so that the dyeing and decoloring bottle rotates, a solution in the dyeing and decoloring bottle is uniformly shaken, and manual shaking is reduced.

Description

Technical Field

[0001] This utility model relates to the field of protein glue Coomassie Brilliant Blue processing technology, specifically a protein glue Coomassie Brilliant Blue rapid staining and decolorization device. Background Technology

[0002] Coomassie Brilliant Blue is a reagent used for staining protein electrophoresis gels. Its main components are Coomassie Brilliant Blue G-250 and Coomassie Brilliant Blue R-250.

[0003] Current experiments involving Coomassie Brilliant Blue staining and destaining with protein gel mostly involve pouring the solution into a large petri dish, placing the protein gel inside, manually shaking the dish, and then heating it in a water bath. However, manually shaking the petri dish is labor-intensive and can result in uneven staining. Therefore, this paper proposes a rapid Coomassie Brilliant Blue staining and destaining device for protein gel. Utility Model Content

[0004] The purpose of this invention is to provide a rapid staining and destaining device for Coomassie Brilliant Blue using protein glue, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a protein gel Coomassie brilliant blue rapid staining and destaining device, including a water bath;

[0006] Four support legs are fixedly connected to the bottom of the water bath tank;

[0007] A through slot is opened on the front of the water bath, and a glass window is fixedly connected to the inner wall of the through slot to observe the dyeing and decolorization process in the dyeing and decolorization bottles.

[0008] The lid is located on the top of the water bath, and the inner wall of the lid is threadedly connected to the inner wall of the water bath by bolts.

[0009] The staining and decolorizing bottles are located inside the water bath.

[0010] A through hole is opened on the top surface of the box lid, and the top surface of the dyeing and decolorizing bottle extends through the top and bottom surfaces of the box lid and the inner wall of the through hole to the top of the box lid;

[0011] And a sealing plug located above the dyeing and decolorizing bottle, the surface of the sealing plug being sealed and squeezed to the inner wall of the dyeing and decolorizing bottle, and a rotating component being provided below the dyeing and decolorizing bottle;

[0012] An exhaust gas treatment component is installed above the cover of the tank.

[0013] Preferably, the rotating assembly includes an annular plate located below the dyeing and decolorizing bottle. A support frame is fixedly connected to the bottom surface of the annular plate. The bottom end face of the dyeing and decolorizing bottle extends through the top surface of the annular plate to the inner side of the annular plate. A motor is fixedly connected to the bottom surface of the water bath. The top end face of the motor output rod extends through the bottom surface of the water bath to the inside of the water bath. The top end face of the motor output rod is fixedly connected to the bottom surface of the support frame. Two clamping plates are provided inside the annular plate. The two clamping plates are symmetrically arranged on the left and right sides of the dyeing and decolorizing bottle. A pull rod is fixedly connected to the right side of the clamping plate. The right end face of the pull rod extends through the inner side of the annular plate to the right side of the annular plate. A spring is sleeved on the surface of the pull rod. The left and right end faces of the spring are fixedly connected to the right side of the clamping plate and the inner side of the annular plate, respectively. A rubber plate is fixedly connected to the inner side of the clamping plate. The inner side of the rubber plate is pressed against the surface of the dyeing and decolorizing bottle. The surface of the dyeing and decolorizing bottle is in contact with the inner wall of the through hole.

[0014] Preferably, the exhaust gas treatment assembly includes a treatment box fixedly connected to the top surface of the box cover. The treatment box is provided with a vent pipe and an exhaust pipe. The exhaust pipe is located to the lower right of the vent pipe. The bottom right end of the vent pipe extends through the top surface of the treatment box and the top surface of the sealing plug to the bottom of the sealing plug. The surface of the vent pipe is rotatably connected to the inner wall of the sealing plug through a bearing seat. The surface of the vent pipe is fixedly connected to the inner wall of the treatment box. The left end of the exhaust pipe extends through the top surface of the treatment box to the top of the treatment box.

[0015] Preferably, the support frame is configured as a cross-shaped plate, which provides support for the ring plate and the dyeing and decolorizing bottles.

[0016] Preferably, an addition tube is provided above the sealing plug, the bottom end of the addition tube is fixedly extended through the top surface of the sealing plug to the bottom of the sealing plug, and a first tube cap is threadedly connected to the top surface of the addition tube to prevent the solution in the dyeing and decolorizing bottle from evaporating.

[0017] Preferably, a water inlet pipe is provided above the cover, and the bottom end of the water inlet pipe is fixedly inserted through the top surface of the cover and extends to the bottom of the cover. A drain pipe is provided on the right side of the water bath, and the left end of the drain pipe is fixedly inserted through the right side of the water bath and extends into the interior of the water bath. A first valve is provided inside the drain pipe, and the drain pipe facilitates the discharge of water from the water bath.

[0018] Preferably, a liquid inlet pipe is provided above the treatment box, and the bottom end of the liquid inlet pipe is fixedly inserted through the top surface of the treatment box and extends into the interior of the treatment box. A liquid outlet pipe is provided on the left side of the treatment box, and the right end of the liquid outlet pipe is fixedly inserted through the left side of the treatment box and extends into the interior of the treatment box.

[0019] Preferably, a second valve is provided inside the drain pipe, and a second cap is threaded onto the top surface of the filling pipe, so that the filling pipe can easily add the treatment liquid that absorbs acetic acid into the treatment tank.

[0020] Compared with the prior art, the beneficial effects of this utility model are:

[0021] 1. This rapid staining and destaining device for Coomassie Brilliant Blue using protein gel involves rotating components and using a clamping plate to drive a rubber plate to clamp and fix the staining and destaining bottle in a ring plate. A motor drives the support frame and the ring plate to rotate, thereby rotating the staining and destaining bottle and uniformly shaking the solution inside the bottle. This reduces the unevenness that occurs with manual shaking and improves the efficiency of the device in staining and destaining Coomassie Brilliant Blue using protein gel.

[0022] 2. This rapid staining and decolorizing device for Coomassie Brilliant Blue using protein glue utilizes a tail gas treatment component and a treatment liquid heated in the treatment chamber that can absorb acetic acid to treat the acetic acid waste gas generated during the staining and decolorizing process of Coomassie Brilliant Blue using protein glue. This avoids environmental pollution from waste gas and improves the environmental friendliness of the device during the staining and decolorizing process of Coomassie Brilliant Blue using protein glue. Attached Figure Description

[0023] Figure 1 This is a front sectional perspective view of the present invention;

[0024] Figure 2 This is a perspective view of the overall main view of this utility model;

[0025] Figure 3 This is a left-side perspective view of the entire utility model;

[0026] Figure 4 This is a front perspective view of the dyeing and decolorizing bottle of this utility model;

[0027] Figure 5 This is a bottom-view perspective view of the support frame of this utility model.

[0028] In the diagram: 1. Water bath tank; 2. Glass window; 3. Cover; 4. Support leg; 5. Dyeing and decolorizing bottle; 60. Rotating component; 601. Motor; 602. Support frame; 603. Ring plate; 604. Clamping plate; 605. Rubber plate; 606. Pull rod; 607. Spring; 608. Water inlet pipe; 609. Drain pipe; 6010. Adding pipe; 61. Exhaust gas treatment component; 611. Ventilation pipe; 612. Treatment box; 613. Exhaust pipe; 614. Liquid adding pipe; 615. Liquid draining pipe; 7. Sealing plug. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] Example 1: Please refer to Figure 1 - Figure 5 This utility model provides a technical solution: a protein gel Coomassie brilliant blue rapid staining and destaining device, including a water bath 1;

[0031] Four support legs 4 are fixedly connected to the bottom of the water bath tank 1;

[0032] A glass window 2 is fixedly connected to the inner wall of the water bath tank 1 through a through groove on the front.

[0033] The cover 3 is located on the top surface of the water bath 1, and the inner wall of the cover 3 is threadedly connected to the inner wall of the water bath 1 by bolts;

[0034] The staining and decolorizing bottle 5 is located inside the water bath 1;

[0035] A through hole is opened on the top surface of the box cover 3, and the top surface of the dyeing and decolorizing bottle 5 extends through the top and bottom surfaces of the box cover 3 and the inner wall of the through hole to the top of the box cover 3.

[0036] And a sealing plug 7 located above the dyeing and decolorizing bottle 5, the surface of the sealing plug 7 is sealed and squeezed to the inner wall of the dyeing and decolorizing bottle 5, and a rotating component 60 is provided below the dyeing and decolorizing bottle 5;

[0037] An exhaust gas treatment component 61 is installed above the cover 3.

[0038] The rotating assembly 60 includes an annular plate 603 located below the dyeing and decolorizing bottle 5. A support frame 602 is fixedly connected to the bottom surface of the annular plate 603. The bottom surface of the dyeing and decolorizing bottle 5 extends through the top surface of the annular plate 603 to the inner side of the annular plate 603. A motor 601 is fixedly connected to the bottom surface of the water bath 1. The top surface of the output rod of the motor 601 extends through the bottom surface of the water bath 1 to the interior of the water bath 1. The top surface of the output rod of the motor 601 is fixedly connected to the bottom surface of the support frame 602. Two clamping plates 604 are provided inside the annular plate 603. The two clamping plates 604 are symmetrically arranged on the left and right sides of the dyeing and decolorizing bottle 5. A pull rod 606 is fixedly connected to the right side of the clamping plate 604. The right end of the pull rod 606 extends through the inner side of the annular plate 603 to the right side of the annular plate 603. A sleeve is fitted on the surface of the pull rod 606. A spring 607 is provided, with its left and right ends fixedly connected to the right side of the clamping plate 604 and the inner side of the ring plate 603, respectively. A rubber plate 605 is fixedly connected to the inner side of the clamping plate 604, and the inner side of the rubber plate 605 is pressed against the surface of the staining and decolorizing bottle 5. The surface of the staining and decolorizing bottle 5 is in contact with the inner wall of the through hole. By rotating the assembly 60, the clamping plate 604 drives the rubber plate 605 to clamp and fix the staining and decolorizing bottle 5 in the ring plate 603. The motor 601 drives the support frame 602 and the ring plate 603 to rotate, thereby causing the staining and decolorizing bottle 5 to rotate. This causes the solution inside the staining and decolorizing bottle 5 to be shaken evenly, reducing the unevenness caused by manual shaking and improving the efficiency of the device for protein gel Coomassie brilliant blue staining and decolorization.

[0039] The support frame 602 is set as a cross-shaped plate.

[0040] An adding tube 6010 is provided above the sealing plug 7. The bottom end of the adding tube 6010 is fixedly inserted through the top surface of the sealing plug 7 and extends to the bottom of the sealing plug 7. A first tube cap is threadedly connected to the top surface of the adding tube 6010.

[0041] A water inlet pipe 608 is provided above the cover 3. The bottom end of the water inlet pipe 608 is fixedly inserted through the top surface of the cover 3 and extends to the bottom of the cover 3. A drain pipe 609 is provided on the right side of the water bath 1. The left end of the drain pipe 609 is fixedly inserted through the right side of the water bath 1 and extends into the interior of the water bath 1. A first valve is provided inside the drain pipe 609.

[0042] Example 2: Based on Example 1, a preferred embodiment of the protein gel Coomassie Brilliant Blue rapid staining and destaining device provided by this utility model is as follows: Figure 1 - Figure 3As shown: The exhaust gas treatment assembly 61 includes a treatment box 612 fixedly connected to the top surface of the box cover 3. The treatment box 612 is equipped with a vent pipe 611 and an exhaust pipe 613. The exhaust pipe 613 is located to the lower right of the vent pipe 611. The bottom right end of the vent pipe 611 extends through the top surface of the inside of the treatment box 612 and the top surface of the sealing plug 7 to the bottom of the sealing plug 7. The surface of the vent pipe 611 is rotatably connected to the inner wall of the sealing plug 7 through a bearing seat. The surface of the vent pipe 611 is fixedly connected to the inner wall of the treatment box 612. The left end of the exhaust pipe 613 is fixedly extended through the top surface of the inside of the treatment box 612 to the top of the treatment box 612. Through the exhaust gas treatment assembly 61, the acetic acid waste gas generated during the protein glue Coomassie Brilliant Blue staining and decolorization process is treated by the treatment liquid heated by the treatment box 612, which can absorb acetic acid, thereby avoiding waste gas pollution and improving the environmental friendliness of the device during protein glue Coomassie Brilliant Blue staining and decolorization.

[0043] A liquid inlet pipe 614 is provided above the treatment box 612. The bottom end of the liquid inlet pipe 614 is fixedly inserted through the top surface of the treatment box 612 and extends into the interior of the treatment box 612. A liquid outlet pipe 615 is provided on the left side of the treatment box 612. The right end of the liquid outlet pipe 615 is fixedly inserted through the left side of the treatment box 612 and extends into the interior of the treatment box 612.

[0044] The drain pipe 615 is equipped with a second valve inside, and the top surface of the filling pipe 614 is threaded with a second pipe cap.

[0045] In use, pull rod 606, pull rod 602 drives clamping plate 604 to move, so that clamping plate 604 squeezes spring 607, placing the dyeing and decolorizing bottle on support frame 602. Release pull rod 602, spring 607 pushes clamping plate 604 to move, so that clamping plate 604 drives rubber plate 605 to clamp and fix dyeing and decolorizing bottle 5 in ring plate 603;

[0046] Move the lid 3 downwards so that the top of the dyeing and decolorizing bottle 5 passes through the through hole, and connect the lid 3 to the water bath 1 with bolts. Move the sealing plug 7 downwards into the dyeing and decolorizing bottle 5.

[0047] The acetic acid-absorbing treatment solution is added to the treatment tank 612 through the liquid addition pipe 614, and the acetic acid-absorbing treatment solution covers the bottom surface of the exhaust pipe 613. Then, an appropriate amount of dyeing solution is added to the dyeing and decolorizing bottle 5 through the addition pipe 6010, and hot water is added to the water bath 1 through the water inlet pipe 608. The motor 601 is turned on, and the output rod of the motor 601 drives the connected support frame 602 to rotate, which in turn drives the ring plate 603 to rotate, thereby causing the dyeing and decolorizing bottle 5 to rotate, thereby shaking the solution in the dyeing and decolorizing bottle 5 and heating it in the water bath.

[0048] Acetic acid gas generated during the heating process of the solution in dyeing and decolorizing bottle 5 enters the treatment chamber 612 through the vent pipe 611. After being adsorbed by the treatment liquid, the gas is discharged through the exhaust pipe 613.

[0049] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A rapid staining and destaining device for Coomassie Brilliant Blue protein gel, comprising a water bath (1). Four support legs (4) are fixedly connected to the bottom of the water bath (1); A through slot is opened on the front of the water bath tank (1), and a glass window (2) is fixedly connected to the inner wall of the through slot. The cover (3) is located on the top surface of the water bath (1), and the inner wall of the cover (3) is threadedly connected to the inner wall of the water bath (1) by bolts; The staining and decolorizing bottle (5) is located inside the water bath (1); The top surface of the dyeing and decolorizing bottle (5) extends through the top and bottom surfaces of the box cover (3) and the inner wall of the through hole to the top of the box cover (3); and a sealing plug (7) located above the dyeing and decolorizing bottle (5), the surface of the sealing plug (7) being sealed and pressed together with the inner wall of the dyeing and decolorizing bottle (5), characterized in that: A rotating component (60) is provided below the dyeing and decolorizing bottle (5); An exhaust gas treatment component (61) is provided above the cover (3).

2. The apparatus for rapid staining and destaining of Coomassie Brilliant Blue using protein gel as described in claim 1, characterized in that: The rotating assembly (60) includes a ring plate (603) located below the dyeing and decolorizing bottle (5). A support frame (602) is fixedly connected to the bottom surface of the ring plate (603). The bottom end face of the dyeing and decolorizing bottle (5) extends through the top surface of the ring plate (603) to the inner side of the ring plate (603). A motor (601) is fixedly connected to the bottom surface of the water bath (1). The top surface of the output rod of the motor (601) extends through the bottom surface of the water bath (1) to the inside of the water bath (1). The top surface of the output rod of the motor (601) is fixedly connected to the bottom surface of the support frame (602). Two clamping plates (604) are provided inside the ring plate (603). The clamping plate (604) is symmetrically arranged on the left and right sides of the dyeing and decolorizing bottle (5). A pull rod (606) is fixedly connected to the right side of the clamping plate (604). The right end of the pull rod (606) extends through the inner side of the ring plate (603) to the right side of the ring plate (603). A spring (607) is sleeved on the surface of the pull rod (606). The left and right ends of the spring (607) are fixedly connected to the right side of the clamping plate (604) and the inner side of the ring plate (603), respectively. A rubber plate (605) is fixedly connected to the inner side of the clamping plate (604). The inner side of the rubber plate (605) is pressed against the surface of the dyeing and decolorizing bottle (5). The surface of the dyeing and decolorizing bottle (5) is in contact with the inner wall of the through hole.

3. The apparatus for rapid staining and destaining of protein gel Coomassie Brilliant Blue according to claim 1, characterized in that: The exhaust gas treatment assembly (61) includes a treatment box (612) fixedly connected to the top surface of the box cover (3). The treatment box (612) is provided with a vent pipe (611) and an exhaust pipe (613). The exhaust pipe (613) is located to the lower right of the vent pipe (611). The bottom right end of the vent pipe (611) extends through the top surface of the inside of the treatment box (612) and the top surface of the sealing plug (7) to the bottom of the sealing plug (7). The surface of the vent pipe (611) is rotatably connected to the inner wall of the sealing plug (7) through a bearing seat. The surface of the vent pipe (611) is fixedly connected to the inner wall of the treatment box (612). The left end of the exhaust pipe (613) is fixedly connected through the top surface of the inside of the treatment box (612) to the top of the treatment box (612).

4. The apparatus for rapid staining and destaining of protein gel Coomassie Brilliant Blue according to claim 2, characterized in that: The support frame (602) is configured as a cross-shaped plate.

5. The apparatus for rapid staining and destaining of Coomassie Brilliant Blue using protein gel according to claim 1, characterized in that: An adding tube (6010) is provided above the sealing plug (7). The bottom end of the adding tube (6010) is fixedly inserted through the top surface of the sealing plug (7) and extends to the bottom of the sealing plug (7). A first tube cap is threadedly connected to the top surface of the adding tube (6010).

6. The apparatus for rapid staining and destaining of Coomassie Brilliant Blue using protein gel according to claim 2, characterized in that: A water inlet pipe (608) is provided above the cover (3). The bottom end of the water inlet pipe (608) is fixedly inserted through the top surface of the cover (3) and extends to the bottom of the cover (3). A drain pipe (609) is provided on the right side of the water bath (1). The left end of the drain pipe (609) is fixedly inserted through the right side of the water bath (1) and extends to the inside of the water bath (1). A first valve is provided inside the drain pipe (609).

7. The apparatus for rapid staining and destaining of Coomassie Brilliant Blue using protein gel according to claim 3, characterized in that: A liquid inlet pipe (614) is provided above the treatment box (612). The bottom end of the liquid inlet pipe (614) is fixedly inserted through the top surface of the treatment box (612) and extends into the interior of the treatment box (612). A liquid outlet pipe (615) is provided on the left side of the treatment box (612). The right end of the liquid outlet pipe (615) is fixedly inserted through the left side of the treatment box (612) and extends into the interior of the treatment box (612).

8. The apparatus for rapid staining and destaining of Coomassie Brilliant Blue using protein gel according to claim 7, characterized in that: The drain pipe (615) is equipped with a second valve inside, and the top surface of the filling pipe (614) is threaded with a second pipe cap.

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