Kit for extracting low-abundance protein in blood

Abstract

The utility model relates to the technical field of kits, in particular to a kit for extracting low-abundance protein in blood, which comprises a shell and an inner bearing seat, the inner bearing seat is of a rectangular frame structure with an upper opening, and the technical key points are as follows: an upper horizontal distance plate and a lower horizontal supporting plate are arranged in the inner bearing seat, a plurality of distance holes are formed in the upper horizontal distance plate, and the lower horizontal supporting plate is arranged in the lower horizontal distance plate; rubber positioning rings are arranged on the hole walls of the fixed-distance holes, positioning grooves corresponding to the fixed-distance holes in a one-to-one mode are formed in the upper surface of the lower horizontal supporting plate, reagent extraction tubes are clamped and fixed in the fixed-distance holes through the rubber positioning rings, the bottom ends of the reagent extraction tubes are inserted into the corresponding positioning grooves, and transducers are fixed between the bottom face of the inner bearing base and the lower horizontal supporting plate. And an ultrasonic generator connected with the transducer is fixed on one side of the outer wall of the inner bearing seat. According to the utility model, the problem that the kit only has the functions of storage and transfer is solved, and the downstream detection speed is obviously improved by additionally arranging a mixed incubation auxiliary structure.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of reagent kit, specifically to a kind of extraction reagent kit of low abundance protein in blood. BACKGROUND

[0002] As a professional technology for finding biomarkers, plasma proteomics has higher clinical conversion value and strong correlation with clinical chemistry parameters. Both serum and plasma can be used for proteomics research. The abundant proteins in plasma include about 60% albumin, about 35% globulin, about 4% fibrinogen, about 1% lipoprotein and about 1% transferrin. There are 22 high-abundance proteins in blood, accounting for about 99% of the total amount of plasma proteins. The remaining 1% of blood proteins is composed of thousands of low-abundance circulating proteins and potential biomarker proteins released by living cells, apoptotic cells and necrotic cells. Due to the signal interference of high-abundance proteins, blood with complex components is more challenging in biomarker screening. Detecting low-abundance proteins often requires a detection challenge of 10-12 orders of magnitude.

[0003] The so-called extraction reagent kit for low-abundance proteins includes multiple extraction reagent tubes, which meet the extraction and detection requirements of multiple blood samples. After the extraction reagent tubes extract the samples, they are sent to the downstream for enrichment processing, and finally to the mass spectrometer for detection. This can reduce the effect of high-abundance proteins on low-abundance protein signal coverage, thereby improving the signal strength of low-abundance proteins.

[0004] CN 216071118 U discloses an extraction reagent kit for low-abundance proteins, which includes a reagent kit, a cover plate, a first storage compartment, a second storage compartment and a third storage compartment. The cover plate is connected to the top rear end of the reagent kit. The first, second and third storage compartments are distributed inside the reagent kit. The diameter of the first storage compartment is smaller than that of the second storage compartment. The inner side wall of the first storage compartment is fixedly distributed with silica gel pads. The two ends of the third storage compartment are fixedly provided with partitions. A through-hole slot is formed in the middle of the two sides of the third storage compartment. A screw rod is threadedly connected inside the through-hole slot. A positioning plate is fixedly arranged at the inner side end of the screw rod. A fixed plate is fixedly arranged at the outer end of the screw rod. A foam inner pad is fixedly arranged at the inner side end of the third storage compartment. This extraction reagent kit for low-abundance proteins is intended to facilitate the classification and storage of low-abundance protein extraction reagent tubes of different sizes and improve the containment stability. However, after the samples are extracted by the reagent kit, they still need to be transported to the downstream detection site for incubation with enrichment nanoparticles, which requires a long incubation time and is not conducive to rapid detection operations. SUMMARY

[0005] The utility model aims to provide a kind of extraction reagent kit of low abundance protein in blood with reasonable structure and reliable use, solve the problem that reagent kit only has the function of receiving and transporting, significantly improve downstream detection speed by adding mixed incubation auxiliary structure.

[0006] The technical scheme of the utility model discloses

[0007] A kind of extraction kit of low abundance protein in blood, including shell and inner bearing seat, the inner bearing seat is the rectangular frame structure of upper opening, its technical key points are as follows: upper horizontal distance plate and lower horizontal support plate are equipped in the inner bearing seat, a plurality of distance holes are equipped on the upper horizontal distance plate, rubber positioning ring is equipped on the hole wall of distance hole, the upper surface of the lower horizontal support plate is equipped with the positioning groove corresponding with distance hole, extraction reagent tube is clamped and fixed by using rubber positioning ring in distance hole, the bottom end of the extraction reagent tube is inserted into corresponding positioning groove, transducer is fixed between the bottom surface of the inner bearing seat and lower horizontal support plate, ultrasonic generator connected with transducer is fixed on the outer wall side of the inner bearing seat.

[0008] The above-mentioned extraction kit of low abundance protein in blood, the outer wall side away from ultrasonic generator of the inner bearing seat is fixed with handle, the shell is the rectangular shell body of side opening, the inner bearing seat is pushed into the shell by the side opening of shell, the ultrasonic generator is located at the inner end of shell, and the handle is located at the side opening of shell.

[0009] The above-mentioned extraction kit of low abundance protein in blood, the top surface of the ultrasonic generator and the top surface of the inner bearing seat are paved with integrated damping pad, the integrated damping pad is attached to the inner top surface of the shell, and has damping anti-drop function.

[0010] The above-mentioned extraction kit of low abundance protein in blood, the lower surface of the upper horizontal distance plate is equipped with distance extension sleeve connected with distance hole, and the rubber positioning ring extends into the distance extension sleeve.

[0011] The above-mentioned extraction kit of low abundance protein in blood, the inner bearing seat is additionally provided with an upper opening receiving cavity to facilitate the accommodation of other extraction tools.

[0012] The utility model has the advantages of

[0013] The utility model adds the mixed incubation auxiliary structure formed by lower horizontal support plate, transducer and ultrasonic generator, after collecting sample and putting into extraction reagent tube, sample mixes together with pre-embedded nano particles and other adjuvants, high-frequency oscillation signal generated by ultrasonic generator is converted into high-frequency mechanical oscillation through transducer, and is transmitted to mixed liquid through lower horizontal support plate and extraction reagent tube, realizes efficient catalytic mixing effect, and then realizes pre-mixed incubation, when being transported to detection laboratory, detection time is saved, so as to significantly improve downstream detection speed. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1It is the structure schematic diagram of the utility model;

[0015] Figure 2 It is the structure schematic diagram of the inner bearing seat of the utility model;

[0016] Figure 3 It is the sectional view of the utility model;

[0017] Figure 4 It is Figure 3 The enlarged view of A part in the middle.

[0018] In the drawing: 1. shell, 2. inner bearing seat, 3. handle, 4. integral type damping pad, 5. ultrasonic generator, 6. end cover, 7. extraction reagent tube, 8. upper horizontal distance plate, 9. lower horizontal support plate, 10. positioning groove, 11. distance hole, 12. storage cavity, 13. transducer, 14. distance extension sleeve, 15. rubber positioning ring. DETAILED DESCRIPTION

[0019] The utility model is described in detail according to the drawing of the specification.

[0020] As Figures 1-4 Indicated, the extraction reagent box of low abundance protein in blood, including shell 1 and inner bearing seat 2, the inner bearing seat 2 is the rectangular frame structure of upper opening.

[0021] Among them, the inner bearing seat 2 is equipped with upper horizontal distance plate 8 and lower horizontal support plate 9, be equipped with a plurality of distance holes 11 on the upper horizontal distance plate 8, be equipped with rubber positioning ring 15 on the hole wall of distance hole 11, in the embodiment, the lower surface of upper horizontal distance plate 8 is equipped with distance extension sleeve 14 corresponding with distance hole 11, the rubber positioning ring 15 extends into distance extension sleeve 14.The upper surface of lower horizontal support plate 9 is equipped with positioning groove 10 corresponding with distance hole 11, the bottom of positioning groove 10 is arc-shaped.Extraction reagent tube 7 is clamped and fixed in distance hole 11 by rubber positioning ring 15, and the bottom end of extraction reagent tube 7 is inserted into the corresponding positioning groove 10.

[0022] The transducer 13 is fixed between the bottom surface of inner bearing seat 2 and lower horizontal support plate 9, and the ultrasonic generator 5 connected with transducer 13 is fixed on one side of the outer wall of inner bearing seat 2.

[0023] In the embodiment, the inner bearing seat 2 is provided with an upper opening receiving cavity 12 away from the ultrasonic generator side, the receiving cavity 12 is fixed with a pull handle 3 away from the ultrasonic generator side, the shell 1 is a side opening rectangular shell, the inner bearing seat 2 is pushed into the shell 1 through the side opening of the shell, the ultrasonic generator 5 is located at the inner end of the shell 1, and the pull handle 3 is located at the side opening of the shell 1. The top surface of the ultrasonic generator 5 and the top surface of the inner bearing seat 2 are paved with an integrated damping pad 4, the integrated damping pad 4 is attached to the inner top surface of the shell 1, and has a damping anti-dropping function.

[0024] Working principle:

[0025] 1. When in use, the inner bearing seat 2 is pulled out, the end cover 6 of the extraction reagent tube 7 is opened, the collected blood sample and other auxiliary agents are sequentially added to the extraction reagent tube 7, and then the end cover 6 is covered.

[0026] 2. Sequentially complete the collection of multiple samples until completion.

[0027] 3. The ultrasonic generator 5 is started, the generated high-frequency oscillation signal is converted into a high-frequency mechanical oscillation through the transducer 13, is transmitted to the mixed liquid of nanoparticles and samples through the lower horizontal support plate 9 and the extraction reagent tube 7, realizes efficient catalytic mixing effect, uses the nanoparticles to pre-incubate the blood sample, the nanoparticles adsorb the protein in the blood to form a protein crown wrapped with nanoparticles, and is used for enrichment of low-abundance proteins in blood.

[0028] 4. During the transportation of the reagent kit, the incubation work is also in progress, and the processing time before downstream detection is greatly shortened.

[0029] The embodiments of the utility model are described in detail above, but the content described can only be the preferred embodiment of the utility model, and cannot be considered as limiting the implementation range of the utility model. Any equivalent change and improvement made in the scope of the utility model creation should still belong to the scope of the utility model.

Claims

1. A kit for extracting low-abundance proteins in blood, comprising a housing and an inner carrier, the inner carrier being an open-top rectangular frame structure, characterized in that: The inner bearing seat is provided with an upper horizontal distance plate and a lower horizontal support plate, the upper horizontal distance plate is provided with a plurality of distance holes, the hole wall of the distance hole is provided with a rubber positioning ring, the upper surface of the lower horizontal support plate is provided with a positioning groove corresponding to the distance hole, the extraction reagent tube is clamped and fixed in the distance hole by the rubber positioning ring, the bottom end of the extraction reagent tube is inserted into the corresponding positioning groove, the bottom surface of the inner bearing seat is fixed with a transducer between the lower horizontal support plate, and the outer wall side of the inner bearing seat is fixed with an ultrasonic generator connected with the transducer.

2. The kit for extracting low-abundance proteins in blood according to claim 1, characterized in that: The outer wall side of the inner bearing seat away from the ultrasonic generator is fixed with a handle, the outer shell is a rectangular shell with a side opening, the inner bearing seat is pushed into the outer shell through the side opening of the outer shell, the ultrasonic generator is located at the inner end of the outer shell, and the handle is located at the side opening of the outer shell.

3. The kit for extracting low-abundance proteins in blood according to claim 1, characterized in that: The top surface of the ultrasonic generator and the top surface of the inner bearing seat are paved with an integrated damping pad, and the integrated damping pad is attached to the inner top surface of the outer shell.

4. The kit for extracting low-abundance proteins in blood according to claim 1, characterized in that: The lower surface of the upper horizontal distance plate is provided with a distance extension sleeve connected with the distance hole, and the rubber positioning ring extends into the distance extension sleeve.

5. The kit for extracting low-abundance proteins in blood according to claim 1, characterized by: The inner bearing seat is additionally provided with an open upper receiving cavity.

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