Reagent subpackaging auxiliary device

Abstract

The utility model provides a reagent sub-packaging auxiliary device, and relates to the technical field of auxiliary tools, the reagent sub-packaging auxiliary device comprises a main plate and an auxiliary plate, the main plate is provided with multiple columns or multiple rows of through grooves with upper and lower openings, each column or each row of through grooves respectively correspond to each column or each row of reagent holes of a deep hole plate, the reagent holes are used for containing reagents, and the auxiliary plate is used for containing the reagents. One auxiliary plate is arranged corresponding to each through groove, the auxiliary plates are connected with the main plate, and at least one end part of each auxiliary plate can move to shield or expose the reagent holes below the through grooves. According to the technical scheme, the technical problems that in the prior art, when a reagent is manually subpackaged to a deep hole plate by an experimenter, mistaken adding, missing adding, excessive adding, slow subpackaging and the like are likely to occur are solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of auxiliary tools, in particular to a reagent sub-packaging auxiliary device. BACKGROUND

[0002] Compared with a pipetting nucleic acid extractor, a magnetic rod nucleic acid extractor has the advantages of fast extraction speed and stable extraction result. More and more manufacturers have begun to research and develop such instruments, and some of the extractors have been put into market use. However, the following problems exist in the process of instrument research and use: no matching reagent in the early stage of research, and some laboratories choose to use bulk reagents. The above situation often requires manual reagent sub-packaging by experimenters, but due to too many and too dense hole positions of deep well plates (such as 96-hole deep well plates), the following problems often occur in the sub-packaging process: wrong addition, omission, excessive addition, and slow sub-packaging. SUMMARY

[0003] The present application provides a reagent sub-packaging auxiliary device to solve the technical problems that experimenters manually sub-packaging reagents to deep well plates in the prior art are prone to wrong addition, omission, excessive addition, and slow sub-packaging. The technical effects produced by the preferred technical solutions in the technical solutions provided by the present application are described in detail below.

[0004] To achieve the above-mentioned purpose, the present application provides a reagent sub-packaging auxiliary device, comprising: a main plate and a sub-plate, a plurality of columns or rows of through grooves with upper and lower openings are formed on the main plate, each column or each row of the through grooves corresponds to each column or each row of reagent holes of a deep well plate, the reagent holes are used to hold reagents, one sub-plate is arranged corresponding to each through groove, the sub-plate is connected with the main plate, and at least one end of the sub-plate can be moved to shield or expose the reagent holes below the through groove.

[0005] Optionally, a slide is arranged on the side wall of each through groove, an opening corresponding to each through groove is formed on the side of the main plate, and each sub-plate is arranged in the slide through the opening.

[0006] Optionally, a limiting protrusion is arranged at the end of the sub-plate in the through groove, and the width of the opening is smaller than the width of the slide.

[0007] Optionally, the limiting protrusion has an inclined surface inclined outward from the end of the sub-plate, and the limiting protrusion can be folded to the side of the sub-plate when the inclined surface is stressed.

[0008] Optionally, a handle for assisting in pushing and pulling the sub-plate is arranged at the end of the sub-plate outside the through groove.

[0009] Optionally, one end of the auxiliary plate is rotatably connected to one side of the upper surface of the main plate, and the other end is buckled to the other side of the main plate.

[0010] Optionally, the end of the auxiliary plate buckled to the main plate is provided with a hook, and the main plate is provided with a card hole corresponding to the hook position when the auxiliary plate is buckled.

[0011] Optionally, the bottom surface of the main plate is provided with a groove, and the main plate is buckled to the upper end of the deep hole plate through the groove.

[0012] Optionally, the four corners of the groove are provided with supporting wood.

[0013] Optionally, the surface of the main plate is provided with a channel mark corresponding to each of the through grooves.

[0014] The technical scheme of the application can include the following beneficial effects:

[0015] The reagent dispensing auxiliary device provided by the application is provided with a main plate and an auxiliary plate, the main plate is provided with a through groove corresponding to the reagent hole of the deep hole plate, an auxiliary plate is provided corresponding to each through groove, and at least one end of the auxiliary plate is movably arranged. Therefore, the reagent hole can be covered or exposed, that is, the auxiliary plate can be moved as needed to facilitate the addition of reagents to the position where the reagents need to be added, thereby preventing misaddition. It can be seen that the reagent dispensing auxiliary device solves the technical problems of misaddition, omission, overaddition, slow dispensing and the like in the prior art when the experimental personnel manually dispense reagents to the deep hole plate. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical scheme in the embodiments of the application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor.

[0017] Figure 1 is a structural schematic diagram of a deep hole plate in an embodiment of the application;

[0018] Figure 2 is a structural schematic diagram of a reagent dispensing auxiliary device in an embodiment of the application;

[0019] Figure 3 is a structural schematic diagram of a reagent dispensing auxiliary device buckled to a deep hole plate in an embodiment of the application;

[0020] Figure 4 is a structural schematic diagram of an auxiliary plate in an embodiment of the application;

[0021] Figure 5is a sectional view of the end of the auxiliary plate in an embodiment of the present application;

[0022] Figure 6 is a structural schematic diagram of a reagent sub-packaging auxiliary device in another embodiment of the present application;

[0023] Figure 7 is a structural schematic diagram of a reagent sub-packaging auxiliary device in another embodiment of the present application when buckled on a deep-well plate;

[0024] Figure 8 is a structural schematic diagram of the bottom of a reagent sub-packaging auxiliary device in an embodiment of the present application.

[0025] In the figure: 1, main plate; 2, auxiliary plate; 3, through slot; 4, slide; 5, handle; 6, limiting protrusion; 7, extension spring; 8, opening; 9, passage mark; 10, support wood; 11, deep-well plate; 12, reagent well; 13, clamping hook; 14, clamping hole. DETAILED DESCRIPTION

[0026] To make the purpose, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described in detail below. Obviously, the described embodiments are only some of the embodiments of the present application, not all. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of the present application.

[0027] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or there can be a middle element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there can be a middle element.

[0028] As shown in Figures 1 to 8 , the present application provides a reagent sub-packaging auxiliary device and provides the following two embodiments.

[0029] Embodiment one:

[0030] The reagent sub-packaging auxiliary device of the present embodiment includes a main plate 1 and an auxiliary plate 2. The main plate 1 is provided with a plurality of columns or rows of through slots 3 with openings from top to bottom. Each column or row of through slots 3 corresponds to each column or row of reagent wells 12 of a deep-well plate 11. The reagent wells 12 are used to hold reagents. An auxiliary plate 2 is provided for each through slot 3. The auxiliary plate 2 is connected to the main plate 1, and at least one end of the auxiliary plate 2 can be moved to cover or expose the reagent wells 12 below the through slot. A slide is provided on the sidewall of each through slot. An opening is provided on the side of the main plate corresponding to the end of each through slot and communicates with the corresponding through slot. An auxiliary plate is provided for each through slot. The auxiliary plate is disposed in the slide through the opening.

[0031] Specifically, the device is provided with a main plate 1 and a sub-plate 2, the main plate 1 is provided with a through groove 3 corresponding to the reagent holes 12 of the deep-well plate, and a sub-plate 2 is arranged on both sides of each through groove 3. The sub-plate 2 is arranged in the slide 4 of the two side walls of the through groove 3, and the sub-plate 2 can be hidden or exposed by sliding in the through groove 3, that is, the sub-plate 2 can be pulled out according to the need to add reagents to the position where the reagents need to be added.

[0032] Further, as shown in the figure, Figure 5 the end of the sub-plate 2 in the through groove 3 is provided with a limiting protrusion 6, and the width of the opening is smaller than the width of the slide. In order to prevent the sub-plate 2 from being pulled out of the through groove 3 during the pulling process. The end of the sub-plate 2 in the through groove 3 is not directly connected with the main plate, and the sub-plate 2 has a drawer function similar to the relationship between the drawer and the case. The drawer function is realized by the cooperation of the slide 4 on the main plate 1 and the sub-plate 2. The front end of the sub-plate 2 is designed as a variable angle, which is used to prevent the sub-plate 2 from being pulled out completely.

[0033] Further, as shown in the figure, Figure 6 the limiting protrusion 6 has an inclined surface inclined outward from the end of the sub-plate 2, and the limiting protrusion 6 can be folded to the side of the sub-plate 8 when the inclined surface is stressed. The two side surfaces of the sub-plate 2 are respectively provided with an opening 8, and the limiting protrusion 6 is arranged in the opening 8. The bottom end of the limiting protrusion 6 abuts against the extension spring 7. When the sub-plate 2 is inserted into the slide 4 through the opening on the front side of the main plate 1, the limiting protrusion 6 is retracted into the opening 8. When the sub-plate 2 moves in the slide 4, the limiting protrusion 6 is located on both sides of the sub-plate 2, preventing the sub-plate 2 from being pulled out of the through groove 3 completely.

[0034] Further, the end of the sub-plate 2 outside the through groove 3 is provided with a handle 5 for assisting in pulling and pushing the sub-plate; the handle 5 is plate-shaped and perpendicular to the surface of the sub-plate 2. The end of the sub-plate 2 is designed with the handle 5 connected thereto, which facilitates the pulling and pushing of the sub-plate 2.

[0035] Further, the bottom surface of the main plate 1 is provided with a groove, and the main plate 1 is buckled to the upper end of the deep-well plate 11 through the groove. By buckling the groove with the main plate 1, the stability between the sub-plate 2 and the main plate 1 is increased.

[0036] Further, as shown in the figure, Figure 4 the four corners of the groove are provided with supporting wood 10. By setting a groove on the bottom surface of the main plate 1, the main plate 1 is buckled with the deep-well plate 11 through the groove, which increases the stability between the main plate 1 and the deep-well plate 11.

[0037] Further, the surface of the main plate 1 is provided with a channel mark 9 corresponding to each through groove 3. The front side above the main plate 1 is provided with a row of numerical marks 1-12 arranged in order from left to right corresponding to the through groove 3. The 12 sub-plates 2 correspond one by one to the 12 columns of reagent hole positions on the deep-well plate 11, which facilitates the identification of the reagent addition hole position.

[0038] Embodiment Two:

[0039] The reagent dispensing auxiliary device of the embodiment includes a main plate 1 and a sub-plate 2. The main plate 1 is provided with a plurality of columns or rows of through grooves 3 with openings upward and downward. Each column or row of the through grooves 3 corresponds to each column or row of reagent holes 12 of a deep-well plate 11. The reagent holes 12 are used to contain reagents. A sub-plate 2 is provided corresponding to each through groove 3. The sub-plate 2 is connected with the main plate 1, and at least one end of the sub-plate 2 can be moved to shield or expose the reagent holes 12 below the through groove. One end of the sub-plate 2 is rotationally connected with one side of the upper surface of the main plate 1, and the other end is bucklingly connected with the other side of the main plate 1.

[0040] The rotationally connected part of the sub-plate 2 and the main plate 1 is provided with a rotating shaft and a torsional spring (not shown in the figure). The sub-plate 2 and the main plate 1 are opened at an angle of 106 degrees without external force. The movable end of each sub-plate 2 is provided with a hook 13, and the side of the main plate 1 is provided with a clamping hole 14 corresponding to the hook 13 of the sub-plate 2. When the sub-plate 2 is pressed to 0 degrees by external force, the hook 13 and the clamping hole 14 are buckled to lock the sub-plate 2. By lifting gently, the sub-plate 2 will be bounced up to return to an angle of 106 degrees. In this embodiment, the structure of the bottom of the main plate 1 and the related structure of the supporting wood 11 are the same as those of the above-mentioned embodiment one, and will not be described here.

[0041] The reagent dispensing auxiliary device in this application can reduce the operation errors of the experimental personnel during the reagent dispensing process and improve the speed of dispensing reagents when the experimental personnel manually dispense and extract reagents.

[0042] For the device of the application, an example of a specific implementation operation case is provided, including the following steps:

[0043] (1) Take out the nucleic acid extraction reagent, the deep-well plate, the device, and the pipettor and Tip head required for dispensing the nucleic acid extraction reagent; place the device above the deep-well plate.

[0044] (2) Pull open / close the sub-plates 1 and 7, and use the pipettor to sequentially add the eluent into the corresponding holes of the deep-well plate.

[0045] (3) Push / close the sub-plates 1 and 7, pull open / close the sub-plates 2, 3, 8, and 9, and use the pipettor to sequentially add the rinsing liquid 2 into the corresponding holes of the deep-well plate.

[0046] (4) Push / close the sub-plates 2, 3, 8, and 9, pull open / close the sub-plates 4 and 10, and use the pipettor to sequentially add the rinsing liquid 1 into the corresponding holes of the deep-well plate.

[0047] (5) Push / close the sub-plates 4 and 10, pull open / close the sub-plates 5 and 11, and use the pipettor to sequentially add the rinsing liquid containing magnetic beads into the corresponding holes of the deep-well plate.

[0048] (6) Push / pull the sub-plates 5 and 11, pull open the sub-plates 6 and 12, and use a pipette to sequentially add proteinase K, lysis solution, and samples into the corresponding holes of the deep-well plate.

[0049] (7) Remove the device, transfer the deep-well plate to a nucleic acid extractor or an all-in-one machine integrating nucleic acid extraction, nucleic acid quality inspection, and library construction, and perform nucleic acid extraction.

[0050] (8) After the nucleic acid extraction is completed, remove the deep-well plate, place the device above the deep-well plate, pull open the sub-plates 1 and 7, and use a pipette to sequentially remove the nucleic acid, and the experiment is completed.

[0051] The technical solution of the present application has the following beneficial effects:

[0052] 1. The technical solution of the present application has simple structure, convenient use, low cost, and wide application range.

[0053] 2. The sub-plate design of the technical solution of the present application facilitates pushing and pulling or opening the sub-plates away from the person, and facilitates the operation of the experimental personnel.

[0054] 3. The technical solution of the present application can effectively reduce the mistakes of the experimental personnel during the reagent dispensing process, and at the same time improve the speed of reagent dispensing.

[0055] In the description of the present application, it should be noted that the orientations or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", "third" are only for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0056] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0057] The above merely provides the specific implementation of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A reagent aliquoting aid, characterized by, The utility model relates to a deep well plate, which comprises: a main plate and a sub-plate, a plurality of columns or rows of through grooves with upper and lower openings are formed on the main plate, each column or row of the through grooves corresponds to each column or row of reagent wells of the deep well plate, the reagent wells are used for containing reagents, one sub-plate is arranged corresponding to each through groove, the sub-plate is connected with the main plate, and at least one end of the sub-plate can be moved to shield or expose the reagent wells below the through groove.

2. The reagent sub-packing assisting device according to claim 1, wherein A slide is arranged on the side wall of each through groove, an opening corresponding to each through groove is formed on the side of the main plate, and the sub-plate is arranged in the slide through the opening.

3. The reagent sub-packing assisting device according to claim 2, wherein The end of the sub-plate in the through groove is provided with a limiting protrusion, and the width of the opening is smaller than the width of the slide.

4. The reagent sub-packing assisting device according to claim 3, wherein The limiting protrusion has an inclined surface inclined outward from the end of the sub-plate, and the limiting protrusion can be folded to the side of the sub-plate when the inclined surface is stressed.

5. The reagent sub-packing assisting device according to claim 2, wherein The end of the sub-plate outside the through groove is provided with a handle for assisting in pushing and pulling the sub-plate.

6. The reagent sub-packing assisting device according to claim 1, wherein One end of the sub-plate is rotatably connected with one side of the upper surface of the main plate, and the other end is buckled with the other side of the main plate.

7. The reagent sub-packing assisting device according to claim 6, wherein The end of the sub-plate buckled with the main plate is provided with a hook, and the main plate is provided with a hole corresponding to the position of the hook when the sub-plate is buckled.

8. The reagent sub-packing assisting device according to any one of claims 1 to 7, characterized by, The bottom surface of the main plate is provided with a groove, and the main plate is buckled to the upper end of the deep well plate through the groove.

9. The reagent sub-packing assisting device according to claim 8, wherein The four corners of the groove are provided with supporting woods.

10. The reagent sub-packing assisting device according to any one of claims 1 to 7, characterized by, A channel mark is arranged on the surface of the main plate corresponding to each through groove.

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