Fermentation tank for secondary propagation of lactic acid bacteria yeast

Abstract

The fermentation tank comprises an inner cylinder and an outer cylinder, the outer surface of the inner cylinder is completely wrapped with an inner cylinder seal head, two groups of spiral jackets with different paths are distributed on the outer wall of the inner cylinder seal head, different steam coils and condensing coils are arranged in each group of spiral jackets, and the steam coils and the condensing coils are distributed in a staggered manner. The inner cylinder end socket is further provided with a disc cover, an acid-base inlet, an inoculation opening, a sterile air inlet, a stirring assembly, a feeding opening and a discharging opening. Through the steam coil and the condensing coil which are distributed in a staggered mode, heating and cooling of the inner cylinder are separated, steam flows from top to bottom, cooling water flows from bottom to top, and therefore it is guaranteed that the temperature change range is small and stable during temperature control. Secondly, sterile air is directly introduced into the inner cylinder through a sterile air inlet and an air inlet pipe, so that the air is in full contact with microorganisms in the stirring process of the stirring assembly, and the fermentation efficiency is further improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to fermentation equipment field especially relates to lactic acid bacteria yeast secondary expansion culture fermentation tank. BACKGROUND

[0002] Lactic acid bacteria yeast secondary expansion culture fermentation is used for improving biomass or specific metabolic product yield process, is often seen in food industry, brewing industry and biotechnology field, and the existing fermentation tank has temperature control system, stirring system, pH value adjustment and oxygen supply, but the existing expansion culture fermentation tank has following defects:

[0003] First, the temperature control of the existing fermentation tank is through steam temperature control, generally is through the heating of the coil to the tank body, makes the constant temperature in the process of fermentation, when needing to reduce temperature, generally still through the heating coil directly injects the cold water, this mode is easy to cause the service life reduction of the coil, second, through the uninterrupted heating and cooling and circulation, makes the control process more complicated, often leads to temperature instability, is not favorable for the expansion culture of microbial species and subsequent fermentation.

[0004] Second, in the expansion culture and fermentation process, generally all sterile air is directly into the tank body, but after the sterile air enters, basically is located above the liquid level, causes the insufficient contact of microorganism fermentation process and material, leads to the reduction of fermentation efficiency. UTILITY MODEL CONTENTS

[0005] The utility model mainly solves lactic acid bacteria yeast expansion culture and the problem of low fermentation efficiency and temperature control instability.

[0006] The utility model adopts the following technical scheme:

[0007] Lactic acid bacteria yeast secondary expansion culture fermentation tank, including inner tube and outer tube, the outer surface of the inner tube is all wrapped inner tube head, and the outer wall of the inner tube head is distributed with two groups of spiral shape clamping sleeves of different paths, one group of spiral shape clamping sleeve is provided with steam coil, another group of spiral shape clamping sleeve is provided with condensing coil, and the steam coil and condensing coil are staggered distribution, the outer tube and inner tube head are still provided with heat preservation rock wool between, the heat preservation rock wool is wrapped spiral shape clamping sleeve, the outer wall of the inner tube is fixedly provided with ear seat support, and the ear seat support all passes through the outer tube and extends outward, the inner tube head is still provided with dish cover, acid and base import, inoculation port, sterile air inlet, stirring assembly, feed inlet and discharge port respectively, and the dish cover, acid and base import, inoculation port, sterile air inlet, stirring assembly, feed inlet and discharge port all are connected with the inner tube, the feed inlet still passes through the outer tube, and the discharge port is located at the bottom of the inner tube.

[0008] Preferably, the bottom of the inner cylinder is further provided with a partition ring, the partition ring is a conical structure, the bottom of the outer cylinder is fixedly connected with the outer wall of the partition ring, and the discharge port is located in the partition ring.

[0009] Preferably, the input end and the output end of the steam coil are connected with a steam inlet and a steam outlet respectively, the steam inlet and the steam outlet are fixed on the outer cylinder, and the steam inlet is located above the steam outlet.

[0010] Preferably, the input end and the output end of the condensing coil are also connected with a cold water inlet and a cold water outlet respectively, the cold water inlet and the cold water outlet are fixed on the outer cylinder, and the cold water inlet is located below the cold water outlet.

[0011] Preferably, the upper portion of the inner cylinder is further provided with a viewing port, a tank washing device, a water inlet, an exhaust port, a temperature sensor, a sterile sampling port, a PH detection port and a safety valve, and the top of the inner cylinder is further provided with symmetrical lifting lugs.

[0012] Preferably, the sterile air inlet is further connected with an air inlet pipe, and the air inlet pipe is fixed in the inner cylinder through a connecting column.

[0013] Preferably, the stirring assembly comprises a stirring motor, a speed reducer, a stirring shaft, a first rotating assembly and a second rotating assembly, the speed reducer is fixed on the upper portion of the inner cylinder head, the speed reducer is cooperatively installed with the stirring motor, the output end of the speed reducer is connected with the stirring shaft, the stirring shaft extends into the inner cylinder, the first rotating assembly and the second rotating assembly are sequentially arranged on the stirring shaft from top to bottom, and the number of the second rotating assembly is not less than two groups.

[0014] Preferably, the first rotating assembly comprises a first mounting seat, a rotating rod and a stirring rod, the first mounting seat is movably installed on the stirring shaft, a plurality of rotating rods are arranged on the first mounting seat, and a plurality of stirring rods are fixedly arranged below each rotating rod.

[0015] Preferably, the second rotating assembly comprises a second mounting seat, a rotating disc and a second stirring paddle, the second mounting seat is also fixedly installed on the stirring shaft, a rotating disc is fixedly installed on the second mounting seat, and a second stirring paddle is arranged on the rotating disc.

[0016] Preferably, the inner cylinder is further provided with a first liquid level meter interface and a second liquid level meter interface, the first liquid level meter interface penetrates through the outer cylinder, and the second liquid level meter interface is located in the partition ring.

[0017] The beneficial effects of the utility model lie in:

[0018] 1. By distributing two sets of spiral jackets with different paths on the outer wall of the inner cylinder head, and setting different steam coils and condenser coils in each set of spiral jackets, and the two are distributed in an alternating manner, the heating and cooling of the inner cylinder are separated, and the steam flows from top to bottom and the cooling water flows from bottom to top. In this way, cooling can be carried out during the heating process, and heating can be carried out during the cooling process. This results in small temperature changes, which is conducive to improving the propagation efficiency and microbial fermentation efficiency.

[0019] 2. Sterile air is directly introduced into the inner cylinder through a sterile air inlet and air inlet pipe, and the air inlet pipe is in direct contact with the material. This allows the air to fully contact the microorganisms during the stirring process of the stirring components, thereby improving the fermentation efficiency. Attached Figure Description

[0020] Figure 1 This is a structural diagram of the secondary expansion fermentation tank for lactic acid bacteria and yeast provided in Example 1;

[0021] Figure 2 This is a diagram of the inner cylinder installation structure in Example 1;

[0022] Figure 3 This is a top view of the structure in Example 1;

[0023] Figure 4 for Figure 1 Cross-sectional view of the KK line;

[0024] Figure 5 This is an enlarged structural diagram of the first rotating component in Example 1;

[0025] Figure 6 This is a structural diagram of another lactic acid bacteria yeast secondary expansion fermentation tank provided in Example 2;

[0026] Figure 7 This is a diagram of the inner cylinder installation structure in Example 2;

[0027] Figure 8 This is a top view of the structure in Example 2;

[0028] Figure 9 for Figure 6 Cross-sectional view of the middle HH line;

[0029] Figure 10 This is an enlarged structural diagram of the first rotating component in Embodiment 2;

[0030] In the figure: inner cylinder 1, outer cylinder 2, inner cylinder head 3, spiral jacket 4, steam coil 5, condensation coil 6, thermal insulation rock wool 7, ear seat support 8, disc cover 9, acid and alkali inlet 10, inoculation port 11, sterile air inlet 12, stirring assembly 13, feed inlet 14, discharge outlet 15, spacer ring 16, steam inlet 17, steam outlet 18, cold water inlet 19, cold water outlet 20, observation port 21, tank washer 22, water inlet 23, exhaust port 24, temperature sensor 25, sterile sampling port 26, PH detection port 27, safety valve 28, lifting lug 29, air inlet pipe 30, connecting column 31, stirring motor 130, speed reducer 131, stirring shaft 132, first rotating assembly 133, second rotating assembly 134, first mounting seat 1330, rotating rod 1331, stirring rod 1332, second mounting seat 1340, rotating disc 1341, second stirring paddle 1342, first liquid level meter interface 32, second liquid level meter interface 33 and thermometer 34. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments.

[0032] Example 1:

[0033] Reference Figures 1-5The application discloses a lactic acid bacteria and yeast secondary expansion fermentation tank, which comprises an inner cylinder and an outer cylinder, the outer surface of the inner cylinder is entirely wrapped with an inner cylinder sealing head, the inner cylinder sealing head mainly plays a role of protecting and transferring heat, two groups of spiral-shaped clamping sleeves with different paths are distributed on the outer wall of the inner cylinder sealing head, the two groups of spiral-shaped clamping sleeves mainly separate heating and cooling to form different channels. In the fermentation process, a condensing coil is located in the middle of a steam coil, so that heating is more uniform, and cooling can be quickly performed when cooling is performed; the steam coil is arranged in one group of spiral-shaped clamping sleeves, the condensing coil is arranged in the other group of spiral-shaped clamping sleeves, the steam coil and the condensing coil are staggered, heat preservation rock wool is arranged between the outer cylinder and the inner cylinder sealing head, the heat preservation rock wool mainly plays a heat preservation role, and the purpose is to ensure that the inner cylinder is not affected by the external environment in the fermentation process; the heat preservation rock wool wraps the spiral-shaped clamping sleeves; ear seat supports are fixedly arranged on the outer wall of the inner cylinder; the bottom of the ear seat supports is supported by supporting rods or supporting columns, so that the fermentation tank is supported as a whole; the ear seat supports all extend outwards through the outer cylinder; a disc cover, an acid-alkali inlet, an inoculation port, a sterile air inlet, a stirring assembly, a feeding port and a discharging port are arranged on the inner cylinder sealing head in a distributed mode and are in communication with the inner cylinder; the feeding port also penetrates through the outer cylinder; raw materials are fed into the inner cylinder through the feeding port during expansion; the stirring assembly is collinear with the center line of the inner cylinder; the stirring assembly stirs the internal raw materials and additives; before stirring, the required acidic or alkaline substances are added through the acid-alkali inlet, the required strains are added into the fermentation tank through the inoculation port, and sterilized air is introduced through the sterile air inlet; fermentation is performed; the discharging port is located at the bottom of the inner cylinder; the discharging mode is automatic discharging under the action of sterile air positive pressure; and the distance between the discharging port and the ground should be higher than 0.4 m.

[0034] A partition ring is further arranged at the bottom of the inner cylinder and is in a conical structure; the bottom of the outer cylinder is fixedly connected with the outer wall of the partition ring; and the discharging port is located in the partition ring.

[0035] The input end and the output end of the steam coil are connected with a steam inlet and a steam outlet respectively; the steam inlet and the steam outlet are fixedly arranged on the outer cylinder and the steam inlet is located above the steam outlet; the steam coil has two functions, one is to provide the required temperature of the fermentation tank, and the other is to sterilize, that is, the inner cylinder can be sterilized at a high temperature of 121-126 DEG C through the steam coil before fermentation; the sterilization needs to sterilize the inner cylinder and the culture solution together for 30 minutes and then keep the temperature for 30 minutes, and the sterilization is generally completed within 3 hours.

[0036] The input end and the output end of the condensing coil 6 are also connected with a cold water inlet 19 and a cold water outlet 20 respectively, and the cold water inlet 19 and the cold water outlet 20 are also fixed on the outer cylinder 2, and the cold water inlet 19 is located below the cold water outlet 20. When the condensing coil 6 is cooling, the cooling liquid flows from the bottom around the condensing coil 6 to cool the inner cylinder 1 upwards, which is different from the traditional top-down cooling. The top-down cooling not only has low cooling effect, but also causes damage to the condensing coil 6 due to the flow rate of the cooling liquid, resulting in reduced service life. Through the bottom-up mode, it is ensured that the cooling liquid can be fully cooled, and the cooling effect is better.

[0037] The upper part of the inner cylinder 1 is also provided with an observation port 21, a tank washer 22, a water inlet 23, an exhaust port 24, a temperature sensor 25, a sterile sampling port 26, a PH detection port 27 and a safety valve 28. The top of the inner cylinder 1 is also symmetrically provided with lifting lugs 29. The tank washer 22 mainly works in the cleaning process of the inner cylinder 1. The temperature sensor 25 mainly detects the fermentation temperature of the fermentation tank, so as to adjust the fermentation temperature of the fermentation tank through the steam coil 5. The sterile sampling port 26 can detect samples during fermentation. The PH detection port 27 mainly detects the PH value of the expansion liquid and the material during the expansion process. The exhaust port 24 mainly discharges the gas generated during the fermentation process and can ensure the pressure inside the fermentation tank, avoiding the failure of the carbon dioxide to be discharged during the fermentation process, resulting in excessive internal pressure. The lifting lugs 29 can facilitate the movement of the fermentation tank during transportation.

[0038] The sterile air inlet 12 is also connected with an air inlet pipe 30, and different air outlets are distributed on the air inlet pipe 30. The air inlet pipe 30 is in a constant internal pressure when no sterile air is introduced, preventing backflow of the material. Only under the action of external pressure, sterile air can be introduced into the air inlet pipe 30 through the sterile air inlet 12, so that the internal pressure increases. In this way, the sterile air will be discharged from the air outlet to contact the material in the inner cylinder 1. The air inlet pipe 30 is fixed in the inner cylinder 1 through a connecting column 31. The sterile air can be directly introduced into the inner cylinder 1 through the air inlet pipe 30, so as to ensure that the sterile air can fully contact the material during the expansion and fermentation process, and the fermentation process is ensured.

[0039] The stirring assembly 13 comprises a stirring motor 130, a speed reducer 131, a stirring shaft 132, a first rotating assembly 133 and a second rotating assembly 134. The speed reducer 131 is fixed on the upper part of the inner cylinder head 3, and is matched with the stirring motor 130. The output end of the speed reducer 131 is connected with the stirring shaft 132. The stirring shaft 132 extends into the inner cylinder 1, and the first rotating assembly 133 and the second rotating assembly 134 are sequentially arranged on the stirring shaft 132 from top to bottom. The number of the second rotating assembly 134 is not less than two.

[0040] The first rotating assembly 133 and the second rotating assembly 134 mainly stir the material in the process of rotating the stirring shaft 132, so that the bacteria in the material can fully contact and react with the sterile air.

[0041] The first rotating assembly 133 comprises a first mounting base 1330, rotating rods 1331 and stirring rods 1332. The first mounting base 1330 is movably mounted on the stirring shaft 132. The first mounting base 1330 is provided with a plurality of rotating rods 1331. Each rotating rod 1331 is fixedly provided with a plurality of stirring rods 1332 below. The first rotating assembly 133 mainly stirs the liquid surface in the upper part of the inner cylinder 1. The stirring process mainly reduces the generation of air bubbles while ensuring that the upper material can also be fully fermented.

[0042] The second rotating assembly 134 comprises a second mounting base 1340, a rotating disc 1341 and a second stirring paddle 1342. The second mounting base 1340 is also fixedly mounted on the stirring shaft 132. The second mounting base 1340 is fixedly mounted with the rotating disc 1341. The second stirring paddle 1342 is obliquely arranged on the rotating disc 1341. The second stirring paddle 1342 is obliquely mounted on the rotating disc 1341, which can reduce the resistance of the second stirring paddle 1342 in the stirring process. It is suitable for high-speed state. If the second stirring paddle 1342 is perpendicular to the rotating disc 1341, the resistance of the second stirring paddle 1342 is larger, which is easy to damage. The second rotating assembly 134 mainly stirs the material from the inside. The stirring can improve the contact between the bacteria in the inside and the air, thereby improving the fermentation efficiency.

[0043] The inner cylinder 1 is also provided with a first liquid level meter interface 32 and a second liquid level meter interface 33. The first liquid level meter interface 32 penetrates the outer cylinder 2, and the second liquid level meter interface 33 is located in the partition ring 16. The first liquid level meter interface 32 and the second liquid level meter interface 33 are mainly used for installing a liquid level meter, which is convenient for monitoring the liquid level of the material in the expansion and fermentation process.

[0044] The inner cylinder 1 is also provided with a thermometer 34. The thermometer 34 can check the temperature detected by the temperature sensor 25. After the temperature sensor 25 is damaged, the thermometer 34 can also detect the temperature, which has higher adaptability.

[0045] Example 2:

[0046] Participation Figures 6-10, embodiment 2 provides another lactic acid bacteria and yeast secondary propagation fermentation tank, which is different from embodiment 1 in that the center line of the stirring assembly 13 is not collinear with the center line of the inner cylinder 1, which is beneficial to propagation. The eccentric arrangement can be beneficial to the slow propagation of the strain. If the collinear arrangement, although the stirring process is more uniform, it is not conducive to strain propagation. Secondly, the overall size of the lactic acid bacteria and yeast secondary propagation fermentation tank provided in embodiment 2 is smaller than that in embodiment 1, and the stirring rods 1332 are vertically and not connected to each other, while in embodiment 1, the stirring rods 1332 are connected to each other at the bottom. During the fermentation process, both are stirring the material liquid surface, but the stirring effect of the stirring rods 1332 that are not connected is lower than that of the form connected through the bottom. After being connected to each other, the stirring amount can be increased, which is convenient for fermentation. Although the stirring amount is small when the bottom is not connected, it will not produce too many bubbles. Both have advantages and disadvantages.

[0047] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art within the technical range disclosed by the present application, according to the technical scheme and the inventive concept of the present application, can make equivalent replacement or change, which should be covered within the protection scope of the present application.

Claims

1. A lactic acid bacteria and yeast secondary propagation fermentation tank comprising an inner cylinder (1) and an outer cylinder (2), characterized in that: The outer surface of the inner cylinder (1) is entirely wrapped by the inner cylinder head (3), and the outer wall of the inner cylinder head (3) is provided with two groups of spiral-shaped jackets (4) with different paths, one group of spiral-shaped jackets (4) is provided with steam coils (5), and the other group of spiral-shaped jackets (4) is provided with condensing coils (6), and the steam coils (5) and the condensing coils (6) are staggered, the outer cylinder (2) and the inner cylinder head (3) are further provided with thermal insulation rock wool (7), the thermal insulation rock wool (7) wraps the spiral-shaped jacket (4), the outer wall of the inner cylinder (1) is fixedly provided with an ear seat support (8), and the ear seat support (8) extends outward through the outer cylinder (2), the inner cylinder head (3) is further provided with a disc cover (9), an acid-alkali inlet (10), an inoculation port (11), a sterile air inlet (12), a stirring assembly (13), a feed inlet (14) and a discharge outlet (15), and the disc cover (9), the acid-alkali inlet (10), the inoculation port (11), the sterile air inlet (12), the stirring assembly (13), the feed inlet (14) and the discharge outlet (15) are in communication with the inner cylinder (1), the feed inlet (14) further passes through the outer cylinder (2), and the discharge outlet (15) is located at the bottom of the inner cylinder (1).

2. The lactic acid bacterial yeast sub-cultivation fermenter according to claim 1, characterized in that: The bottom of the inner cylinder (1) is further provided with a partition ring (16), and the partition ring (16) is a conical structure, the bottom of the outer cylinder (2) is fixedly connected with the outer wall of the partition ring (16), and the discharge outlet (15) is located in the partition ring (16).

3. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 1, characterized in that: The input end and the output end of the steam coil (5) are connected with a steam inlet (17) and a steam outlet (18) respectively, the steam inlet (17) and the steam outlet (18) are fixed on the outer cylinder (2), and the steam inlet (17) is located above the steam outlet (18).

4. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 1, characterized in that: The input end and the output end of the condensing coil (6) are also connected with a cold water inlet (19) and a cold water outlet (20) respectively, the cold water inlet (19) and the cold water outlet (20) are also fixed on the outer cylinder (2), and the cold water inlet (19) is located below the cold water outlet (20).

5. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 1, characterized in that: The upper part of the inner cylinder (1) is further provided with an observation port (21), a tank washing device (22), a water inlet (23), an exhaust port (24), a temperature sensor (25), a sterile sampling port (26), a PH detection port (27) and a safety valve (28), and the top of the inner cylinder (1) is further provided with a lifting lug (29) symmetrically.

6. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 1, characterized in that: The sterile air inlet (12) is further connected with an air inlet pipe (30), and the air inlet pipe (30) is fixed in the inner cylinder (1) through a connecting column (31).

7. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 1, characterized in that: The stirring assembly (13) comprises a stirring motor (130), a speed reducer (131), a stirring shaft (132), a first rotating assembly (133) and a second rotating assembly (134), the speed reducer (131) is fixed on the upper portion of the inner cylinder head (3), the speed reducer (131) is installed in cooperation with the stirring motor (130), the output end of the speed reducer (131) is connected with the stirring shaft (132), the stirring shaft (132) extends into the inner cylinder (1), and the first rotating assembly (133) and the second rotating assembly (134) are sequentially arranged on the stirring shaft (132) from top to bottom, and the number of the second rotating assembly (134) is not less than two groups.

8. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 7, characterized in that: The first rotating assembly (133) comprises a first mounting seat (1330), a rotating rod (1331) and a stirring rod (1332), the first mounting seat (1330) is movably installed on the stirring shaft (132), a plurality of rotating rods (1331) are arranged on the first mounting seat (1330), and a plurality of stirring rods (1332) are fixedly arranged below each rotating rod (1331).

9. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 7, characterized in that: The second rotating assembly (134) comprises a second mounting seat (1340), a rotating disc (1341) and a second stirring paddle (1342), the second mounting seat (1340) is also fixedly installed on the stirring shaft (132), the rotating disc (1341) is fixedly installed on the second mounting seat (1340), and the second stirring paddle (1342) is arranged on the rotating disc (1341).

10. The lactic acid bacteria-yeast secondary propagation fermenter according to claim 2, characterized in that: The inner cylinder (1) is also provided with a first liquid level meter interface (32) and a second liquid level meter interface (33), and the first liquid level meter interface (32) is located above the second liquid level meter interface (33).

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