Preparation of protoplasts and conversion of functional islands

Abstract

The utility model belongs to the technical field of genetic gene experiment devices, and particularly relates to a protoplast preparation and transformation functional island. The filtering module is arranged at the top end of the integrating table, and the interior of the integrating table communicates with the outside through the filtering module; the transfer module is arranged in the center of the interior of the integration table, and the transfer module is used for transferring the protoplast and the consumables; the storage module is arranged in the integration table, and the storage module is used for storing the protoplast and the consumables; the preparation and conversion module is arranged in the integration table, and the preparation and conversion module is used for preparing and converting the protoplast; the preparation and conversion module and the storage module are located on the periphery of the transfer module. According to the utility model, the accurate control of key steps such as enzymolysis, counting, culture and conversion can be realized, the experiment efficiency and repeatability are obviously improved, the dosage of experiment reagents is reduced through miniaturization and parallel processing, and high-throughput parallel processing is realized.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of genetic experiment device, especially to the protoplast preparation and transformation function island. BACKGROUND

[0002] Plant protoplast is the cell without cell wall or naked cell surrounded by plasma membrane, including cell membrane, cytoplasm and nucleus. Because protoplast has no cell wall, it can be directly hybridized, and the distant hybridization incompatibility barrier can be easily overcome. At the same time, because protoplast has no cell wall protection, the damage of nuclease to foreign DNA is avoided, and it is easy to uptake organelles, proteins and other foreign macromolecules, which provides a new way for plant genetic transformation. Therefore, protoplast is considered as an ideal material for cell physiology, developmental biology, cell biology and cell genetics research.

[0003] Protoplast experiment usually includes two steps of preparation and transfection, and its efficiency and stability directly affect the reliability of experimental results. The traditional manual preparation method has problems such as complicated operation, large batch difference, easy protoplast rupture and low experimental throughput, which is difficult to meet the demand of high-throughput experiment.

[0004] Therefore, it is necessary to design a protoplast preparation and transformation function island to solve the above problems. CONTENT OF THE UTILITY MODEL

[0005] The utility model aims at providing a protoplast preparation and transformation function island, realizing accurate control of key steps such as enzymolysis, counting, culture and transformation, significantly improving experimental efficiency and repeatability, and reducing experimental reagent consumption through miniaturization and parallel processing to realize high-throughput parallel processing.

[0006] In order to achieve the above-mentioned purpose, the utility model provides the following scheme: a protoplast preparation and transformation function island, comprising: an integration table; a filter module is arranged at the top end of the integration table, and the inside of the integration table is communicated with the outside through the filter module; a transfer module is arranged in the center of the inside of the integration table, and the transfer module is used for transferring protoplast and consumables; a storage module is arranged in the inside of the integration table, and the storage module is used for storing protoplast and consumables; a preparation and transformation module is arranged in the inside of the integration table, and the preparation and transformation module is used for preparing and transforming protoplast; the preparation and transformation module and the storage module are located at the periphery of the transfer module.

[0007] Based on the protoplast preparation and transformation function island of the utility model, the storage module includes an automatic incubator, a refrigerator and two consumable stacks, and the automatic incubator, the refrigerator and the consumable stacks are located at the periphery of the transfer module.

[0008] The utility model provides a kind of protoplast preparation and conversion function island based on the utility model, the preparation conversion module includes preparation conversion part and detection part, the preparation conversion module is used to prepare and convert protoplast, the detection part is used to detect the protoplast of preparation conversion, the preparation conversion part and the detection part are located at the periphery of the transport module four quarters.

[0009] The utility model provides a kind of protoplast preparation and conversion function island based on the utility model, the preparation conversion part includes preparation liquid workstation, conversion liquid workstation, oscillation incubator, electric transfer instrument device, centrifugal tube switch cover machine, flow cytometer, automatic centrifuge, plate centrifuge, centrifugal concentrator located at the periphery of the transport module four quarters.

[0010] The utility model provides a kind of protoplast preparation and conversion function island based on the utility model, the detection part includes chemiluminescence detector, cell counter, and the chemiluminescence detector and the cell counter are located at the periphery of the transport module four quarters.

[0011] The utility model provides a kind of protoplast preparation and conversion function island based on the utility model, the transport module includes a plurality of slide rail track robots, and a plurality of the slide rail track robots are arranged in parallel in the inside center of the integrated table.

[0012] The utility model provides a kind of protoplast preparation and conversion function island based on the utility model, the filter module includes a plurality of filters, and a plurality of the filters are arranged in array in the top end of the integrated table.

[0013] The utility model provides a kind of protoplast preparation and conversion function island based on the utility model, robot interactive site is further provided in the integrated table, and the integrated table is interacted with another integrated table through the robot interactive site.

[0014] The utility model provides a kind of protoplast preparation and conversion function island based on the utility model, three-color lamp buzzer is further provided in the top end of the integrated table side wall.

[0015] Compared with prior art, the utility model has the following advantages and technical effects: the utility model realizes the accurate control of key steps such as enzymolysis, counting, culture and conversion through standardization program, significantly improves experimental efficiency and repeatability, reduces experimental reagent consumption through miniaturization and parallel processing, realizes high-throughput parallel processing, provides key technical support for gene editing, promoter activity analysis, protein interaction research and other frontier subjects. The utility model not only can automatically complete protoplast enzymolysis, separation, purification, transfection, screening and identification and other experiments, but also can effectively improve the accuracy and repeatability of experimental results, improve experimental throughput and reduce labor cost, so as to meet the application requirements of large-scale experiments. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed in the embodiments. Obviously, the drawings described below are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0017] Figure 1 It is an internal structure plan view of the present application.

[0018] Figure 2 It is an appearance schematic view of the present application.

[0019] Among them, 1, preparation liquid workstation; 2, conversion liquid workstation; 3, shaking incubator; 4, electrotransformation device; 5, centrifugal tube switch cover machine; 6, chemiluminescence detector; 7, flow cytometer; 8, automatic centrifuge; 9, plate centrifuge; 10, automatic incubator; 11, refrigerator; 12, consumable stack; 13, cell counter; 14, centrifugal concentrator; 15, sliding rail track robot; 16, filter; 17, robot interaction site; 18, three-color light buzzer; 19, mobile robot; 20, integration platform. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0021] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the present application will be further described in detail below with reference to the drawings and specific embodiments.

[0022] Referring to Figures 1-2 The present application provides a protoplast preparation and transformation function island, comprising: an integration platform 20; a filtration module arranged at the top end of the integration platform 20, and the inside of the integration platform 20 being in communication with the outside through the filtration module; a transfer module arranged in the center inside the integration platform 20, the transfer module being used for transferring protoplasts and consumables; a storage module arranged inside the integration platform 20, the storage module being used for storing protoplasts and consumables; a preparation and transformation module arranged inside the integration platform 20, the preparation and transformation module being used for preparing and transforming protoplasts; and the preparation and transformation module and the storage module being located at the periphery of the transfer module.

[0023] The integration table 20 is the bearing core of the whole island equipment, adopts 304 stainless steel integrated structure, has the characteristics of corrosion resistance and easy cleaning. The bottom of the integration table 20 is provided with a hidden wiring groove, the independent separation channels of the power line and the signal line are built-in, the signal interference is avoided and the line is not exposed; two auxiliary loading tables of pulling type and rotating type are configured, the pulling type loading table bears 50kg, the rotating type loading table bears 30kg, which is convenient for experimenters to supply consumables and maintain equipment; LED lighting lamp and 254nm ultraviolet lamp module are integrated, the brightness of the LED lighting lamp is 300lux, which meets the operation lighting demand, the ultraviolet lamp covers an area of 90% of the table surface, the disinfection cycle can be set through the central control system, the automatic disinfection is started, the single disinfection time is 30min, and the cleanliness of the experimental environment is ensured.

[0024] Further, the storage module includes an automatic incubator 10, a refrigerator 11 and two consumable stacks 12, and the automatic incubator 10, the refrigerator 11 and the consumable stacks 12 are located at the periphery of the transfer module.

[0025] The sample inlet and outlet of the automatic incubator 10 can interact with the transfer module. The automatic incubator 10 is built-in with a consumable carrier, and the cavity can accurately control the temperature. The carrier movement and sample access actions of the automatic incubator 10 are controlled by the central control system, and the automatic access and real-time management of the sample plate can be realized.

[0026] The refrigerator 11 is an automatic 4℃ refrigerator, and the sample inlet and outlet of the refrigerator 11 can interact with the transfer module. The refrigerator 11 is built-in with a consumable carrier, and the cavity is refrigerated to meet the low-temperature storage requirements of reagents or samples. The carrier movement and sample access actions of the refrigerator 11 are controlled by the central control system, and the automatic access and real-time management of the sample plate can be realized.

[0027] The two consumable stacks 12 are respectively located at the side of the preparation liquid workstation 1 and the transformation liquid workstation 2, and can meet the storage of multiple types of consumables such as deep well plates, shallow well plates and pipette tips. The function island is configured with a self-scanner, which can automatically identify the bar code of the consumables; through the Internet of Things communication module and the central control system, data interconnection is realized, combined with the cooperative work of the transfer module, automatic identification, inventory warning, accurate retrieval and supply of consumables are realized.

[0028] Further, the preparation and transformation module includes a preparation and transformation part and a detection part, and is used for preparing and transforming protoplasts. The detection part is used for detecting the prepared and transformed protoplasts, and the preparation and transformation part and the detection part are located at the periphery of the transfer module.

[0029] Further, the preparation and transformation part includes a preparation liquid workstation 1, a transformation liquid workstation 2, a shaking incubator 3, an electrotransformation instrument 4, a centrifugal tube cap opening and closing machine 5, a flow cytometer 7, an automatic centrifuge 8, a plate centrifuge 9 and a centrifugal concentration machine 14 located at the periphery of the transfer module.

[0030] Preparation liquid station 1 is equipped with pipetting channel, carrying clamp, tilt plate, consumable plate, interactive plate, liquid pipetting, consumable carrying, consumable tilt liquid suction operation can be carried out, through the interactive plate and the transfer module to interact, consumable in and out of the transfer; mainly to carry out the experiment of protoplast enzymolysis, cleaning, counting, concentration equalization, etc.

[0031] Transformation liquid station 2 is equipped with pipetting channel, carrying clamp, temperature control module, oscillation module, consumable plate, interactive plate, liquid pipetting, consumable carrying, low temperature preservation, oscillation incubation operation can be carried out, through the interactive plate and the transfer module to interact, complete the in and out of the transfer of consumables.

[0032] Oscillating incubator 3 is equipped with temperature control module and oscillation plate, which can complete the protoplast oscillation experiment under the condition of avoiding light.

[0033] The electroporator device 4 is a 96-well high-throughput electroporator with an external switch device, which can automatically complete the in and out of the 96-well electroporation plate and the electroporation experiment through the central control instruction.

[0034] The centrifuge tube switch cover machine 5 is used for opening or closing the 50ml centrifuge tube, and the transfer module transfers the centrifuge tube carrier into the centrifuge tube switch cover machine 5 interaction site, and the centrifuge tube switch cover machine 5 can automatically complete the opening or closing action of the centrifuge tube, providing support for subsequent centrifugation operation.

[0035] Flow cytometer 7 is equipped with a sample loading device, and the transfer module places the flow tube carrier in the sample loading device interaction site, and the sample loading device can transfer the flow tube to the flow cytometer 7 sample loading bin to execute the cell sorting function. The flow cytometer 7 can process one flow tube at a time, and can sort the sample into a sample tube or a 96-well plate for subsequent experiments.

[0036] Automatic centrifuge 8 is configured with automatic door opening and closing function; the transfer module puts the centrifuge tube into the centrifuge tube basket in the sample loading bin through the vertical clamp, and the automatic centrifuge 8 automatically positions and realizes the centrifugation function. The automatic centrifuge 8 is built-in temperature control module, which can adjust the temperature in the centrifuge cavity; contains four hangers, each hanger can place four centrifuge tubes.

[0037] Plate centrifuge 9 is built-in 2 centrifuge plate hangers, which can simultaneously realize centrifugation operation on 2 hole plates. The sample loading bin port can be automatically opened and closed, and the transfer module places the sample hole plate on the sample loading bin plate after the transfer module places the sample hole plate on the sample loading bin plate. Through the central control instruction, the centrifugation operation is automatically completed. Two centrifuge plate positions can be automatically positioned, and the working plate position is rotated to the sample loading bin, which is convenient for sample placement and removal.

[0038] The centrifugal concentrator 14 is used for concentrating and purifying the plasmid sample. The liquid is evaporated by heating the centrifugal cavity, the steam is guided out to the external cold trap through the air pipeline and is frozen, and the sample is dried and concentrated. The centrifugal door of the centrifugal concentrator 14 can be automatically opened and closed, the internal basket can be automatically positioned and recognized, and the sample is sent into the centrifugal basket through the transfer module to complete the operation.

[0039] Further, the detection part includes a chemiluminescence detector 6 and a cell counter 13, and the chemiluminescence detector 6 and the cell counter 13 are located at the periphery of the transfer module.

[0040] The chemiluminescence detector 6 integrates functions and modules such as heating, oscillation, liquid addition and chemiluminescence detection. The sample plate position can be automatically ejected and withdrawn, and the interaction with the transfer module is realized. Through the instruction issued by the central control, the liquid addition, heating and oscillation incubation and chemiluminescence detection and other operations can be automatically completed.

[0041] The cell counter 13 is provided with an automatic loading device. The transfer module transports the cell counting plate and its carrier to the automatic loading device temporary storage position through interaction. The automatic loading device loads the cell counting plate into the sample loading chamber of the cell counter 13 to perform the cell counting experiment.

[0042] Further, the transfer module includes a plurality of slide rail track robots 15, and the plurality of slide rail track robots 15 are arranged in parallel in the center of the integrated table 20.

[0043] The slide rail track robot 15 is a 6-axis mechanical arm robot, which is the core of the island transfer. One is arranged in the protoplast preparation area and the protoplast transformation area, and is laid along the high-precision slide rail. The 6-axis mechanical arm has a 360-degree full-space grabbing capability, and is mainly responsible for the automatic transmission and transfer of consumables and sample plates between instruments. The action instruction is automatically issued by the central control system according to the experimental process.

[0044] Further, the filter module includes a plurality of filters 16, and the plurality of filters 16 are arranged in an array at the top end of the integrated table 20.

[0045] The filter 16 selects a HEPA H14 high-efficiency air filter, and the number is 4 sets. The filter 16 is installed on the top of the island body and is connected with the island space environment control system through the positive pressure air supply system, so as to maintain the micro-positive pressure environment in the island, effectively block the dust and microorganisms in the external environment, and ensure the cleanliness of the experimental environment.

[0046] Further, the integrated table 20 is also provided with a robot interaction site 17, and the integrated table 20 interacts with another integrated table 20 through the robot interaction site 17.

[0047] The robot interaction site 17 is fixed at the edge area of the table top and serves as a connection node for the "island-in-island-out" transfer, and can be precisely docked with the mobile robot 19.

[0048] The mobile robot 19 performs the cross-island transfer operation according to the instruction, and moves in the special AGV channel outside the island body, and is used for sample transmission, consumable replenishment transfer and experiment series connection between different working islands.

[0049] Further, the three-color light buzzer 18 is also arranged at the top end of the side wall of the integration table 20.

[0050] The three-color light buzzer 18 is installed around the top edge of the integration table 20, and is linked with the alarm unit of the central control system through a signal cable. The module is configured with blue, green and red three-color indicator lights and a buzzer component. The state display accurately corresponds to the experiment process: blue when the experiment is static or paused, green when the experiment is normally running, and red when the experiment has an error, and the buzzer alarm is triggered synchronously, which directly indicates the equipment running state and guides the experiment personnel to intervene and handle in time.

[0051] In the top view direction, Figure 1 The arrangement of the utility model is as follows: two parallel slide rail track robots 15 are arranged at the center position, from left to right on the top are a consumable stack 12, a chemiluminescence detector 6, an automatic incubator 10, a centrifugal tube cap switching machine 5, another consumable stack 12, a plate-type centrifugal machine 9 is parallel to the chemiluminescence detector 6 and is between the chemiluminescence detector 6 and the slide rail track robot 15, an automatic centrifugal machine 8 is parallel to the centrifugal tube cap switching machine 5 and is between the centrifugal tube cap switching machine 5 and the slide rail track robot 15, from left to right on the bottom are a refrigerator 11, a robot interaction site 17, a centrifugal concentrator 14, a shaking incubator 3, a cell counter 13 and a flow cytometer 7, an electrotransformation instrument device 4 is parallel to the robot interaction site 17 and is between the robot interaction site 17 and the slide rail track robot 15, a preparation liquid workstation 1 is located between the flow cytometer 7 and the consumable stack 12, and a transformation liquid workstation 2 is located below the other consumable stack 12.

[0052] The working process of the utility model is as follows: the mobile robot 19 transfers the experimental consumables and experimental samples from the outside to the robot interaction site 17, and the island slide rail track robot 15 transfers them to the consumable stack 12, the automatic incubator 10 or the refrigerator 11 for temporary storage.

[0053] Plasmid concentration: The 96-well plasmid plate temporarily stored in the refrigerator 11 is sequentially taken out by the slide rail track robot 15 and placed in the centrifugal concentration machine 14 to concentrate the plasmid, so that the plasmid concentration meets the demand of the protoplast transformation experiment. After concentration, it is sent back to the refrigerator 11 by the slide rail track robot 15, waiting for the transfection experiment.

[0054] Protoplast preparation experiment: The reagent tank prepared in advance in the consumable stack 12 is transported to the preparation liquid workstation 1 by the slide rail track robot 15, and the preparation liquid workstation 1 adds enzyme solution to the reagent tank containing plant tissue materials. After mixing, the slide rail track robot 15 transports it to the shaking incubator 3 for dark shaking culture, which is used for tissue enzymolysis and protoplast release.

[0055] After the shaking culture is completed, the slide rail track robot 15 transports it to the preparation liquid workstation 1, and the preparation liquid workstation 1 transfers the digested enzyme solution to a 50ml centrifuge tube and removes large volume impurities through a cell screen. The 50ml centrifuge tube is placed in the centrifuge tube cap opening and closing machine 5 from the preparation liquid workstation 1 by the slide rail track robot 15. After removing the cell screen, the cap is closed and placed in the automatic centrifuge 8 to collect the protoplast by centrifugation. After centrifugation, the centrifuge tube cap opening and closing machine 5 is opened and returned to the preparation liquid workstation 1 by the slide rail track robot 15. The supernatant is removed, W5 liquid is added for resuspension and washing, and centrifugation is performed again. The preparation liquid workstation 1 removes the supernatant, adds MMG liquid, resuspends, takes a small amount of cell suspension, adds cell staining liquid, and places it in a cell counting plate. The cell counting plate is transferred to the sample plate position of the cell counter 13, and the sample is loaded into the cell counter 13 through the sample loading device. Cell counting is performed to obtain the protoplast concentration data. After calculating the concentration and dilution factor, the preparation liquid workstation 1 dilutes the protoplast stock solution and dispenses it into a 96-well plate, and the preparation is completed.

[0056] Protoplast transfection: PEG transfection: The prepared protoplast is transported to the transformation liquid workstation 2 by the slide rail track robot 15, and the concentrated plasmid plate is transferred from the refrigerator 11 to the transformation liquid workstation 2.

[0057] The transformation liquid workstation 2 mixes the plasmid and protoplast, and adds a PEG solution for incubation culture. After the time ends, the mixed liquid in the 96-well plate is added with W5 solution, mixed, and then placed into the plate centrifuge 9 by the slide rail track robot 15 for centrifugation. After the centrifugation ends, the slide rail track robot 15 is used to transfer the mixed liquid back to the transformation liquid workstation 2, remove the supernatant, add W5 resuspension, and then place it into the plate centrifuge 9 by the slide rail track robot 15 for centrifugation. After the centrifugation ends, the slide rail track robot 15 is used to transfer the mixed liquid back to the transformation liquid workstation 2, add W5 resuspension, and then transfer it to the automatic incubator 10 for overnight static culture.

[0058] Electrotransformation: The slide rail track robot 15 is used to transfer the prepared protoplast to the transformation liquid workstation 2, and the concentrated plasmid plate is transferred from the refrigerator 11 to the transformation liquid workstation 2.

[0059] The transformation liquid workstation 2 mixes the plasmid and protoplast, and transfers it to a pre-cooled 96-well electrotransformation plate. The slide rail track robot 15 is used to transfer the 96-well electrotransformation plate from the transformation liquid workstation 2 to the electrotransformation device 4, and the automatic device closes the electrotransformation chamber for an electrotransformation program. After the electrotransformation ends, the automatic device opens the electrotransformation chamber, and the slide rail track robot 15 is used to transfer it back to the transformation liquid workstation 2. The transformation liquid workstation 2 transfers the mixed liquid from the electrotransformation plate to a 96-well plate, adds W5 solution for mixing, and places it into the plate centrifuge 9 by the slide rail track robot 15 for centrifugation. After the centrifugation ends, the slide rail track robot 15 is used to transfer the mixed liquid back to the transformation liquid workstation 2, remove the supernatant, add W5 resuspension, and then transfer it to the automatic incubator 10 for overnight static culture.

[0060] Identification: The mixed plasmid-transformed and cultured protoplast is transferred out of the automatic incubator 10 by the slide rail track robot 15, and then transferred into the preparation liquid workstation 1. The preparation liquid workstation 1 is used to transfer the protoplast into a flow tube, which is then transferred from the preparation liquid workstation 1 to the sample loading device of the flow cytometer 7 by the slide rail track robot 15. The sample loading device places the flow tube into the flow cytometer 7, and the flow cytometer 7 performs sample sorting to screen out positive protoplast that has been successfully transformed and expresses a signal. The positive protoplast is then subjected to external sequencing identification.

[0061] Chemiluminescence detection: The slide rail track robot 15 is used to transfer the transformed and cultured protoplast out of the automatic incubator 10 and into the transformation liquid workstation 2. The transformation liquid workstation 2 is used to transfer the protoplast into a 96-well enzyme-labeled plate, add cell lysis solution to release internal expression products, and then add chemiluminescent substrate. The slide rail track robot 15 is used to transfer the 96-well enzyme-labeled plate from the transformation liquid workstation 2 to the chemiluminescence detector 6, and the chemiluminescence detector 6 is used to determine the luminescence of each well. The data is transmitted back to the central control.

[0062] In the description of the utility model, need understanding is, the orientation or position relation that the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate is based on the orientation or position relation shown in the drawing, just is for the convenience of describing the utility model, and is not indicate or imply the device or element that is indicated must have a particular orientation, construct and operate with a particular orientation, therefore can not be understood as the limitation to the utility model.

[0063] The above-described embodiments are only preferred modes of the utility model, and do not limit the scope of the utility model, and various deformations and improvements of the technical scheme of the utility model made by those skilled in the art without departing from the design spirit of the utility model should fall within the protection scope of the utility model.

Claims

1. A protoplast preparation and transformation functional island, characterized by, The integrated platform (20) comprises: a filter module arranged at the top end of the integrated platform (20), the inside of the integrated platform (20) being in communication with the outside through the filter module; a transfer module arranged at the center of the inside of the integrated platform (20), the transfer module being used for transferring protoplasts and consumables; a storage module arranged in the inside of the integrated platform (20), the storage module being used for storing protoplasts and consumables; a preparation and transformation module arranged in the inside of the integrated platform (20), the preparation and transformation module being used for preparing and transforming protoplasts; the preparation and transformation module and the storage module are located around the periphery of the transfer module; the transfer module comprises a plurality of slide rail track robots (15), and the plurality of slide rail track robots (15) are arranged side by side at the center of the inside of the integrated platform (20); the filter module comprises a plurality of filters (16), and the plurality of filters (16) are arranged in an array at the top end of the integrated platform (20). The storage module comprises an automatic incubator (10), a refrigerator (11) and two consumable stacks (12), and the automatic incubator (10), the refrigerator (11) and the consumable stacks (12) are located around the periphery of the transfer module.

2. The protoplast preparation and transformation functional island according to claim 1, wherein, The preparation and transformation module comprises a preparation and transformation part and a detection part, the preparation and transformation module being used for preparing and transforming protoplasts, the detection part being used for detecting the prepared and transformed protoplasts, and the preparation and transformation part and the detection part being located around the periphery of the transfer module.

3. The protoplast preparation and transformation functional island according to claim 1, wherein, The preparation and transformation part comprises a preparation liquid workstation (1), a transformation liquid workstation (2), a shaking incubator (3), an electrotransformation device (4), a centrifugal tube cap opening and closing machine (5), a flow cytometer (7), an automatic centrifuge (8), a plate centrifuge (9) and a centrifugal concentrator (14) located around the periphery of the transfer module.

4. The protoplast preparation and transformation functional island according to claim 3, wherein, The detection part comprises a chemiluminescence detector (6) and a cell counter (13), and the chemiluminescence detector (6) and the cell counter (13) are located around the periphery of the transfer module.

5. The protoplast preparation and transformation functional island according to claim 3, wherein, The integrated platform (20) further comprises a robot interaction site (17), and the integrated platform (20) interacts with another integrated platform (20) through the robot interaction site (17).

6. The protoplast preparation and transformation functional island of claim 1, wherein, The top end of the side wall of the integrated platform (20) further comprises a three-color light buzzer (18).

7. The protoplast preparation and transformation functional island of claim 1, wherein, ​

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