Automatic workstation for organ-like culture and drug sensitivity detection

Abstract

The utility model relates to the field of biological medicine, in particular to an automatic workstation for organoid culture and drug sensitivity detection. Comprising a rack, a pretreatment module for preparing a sample tissue into a cell suspension, an automatic culture module for culturing the cell suspension into an organoid, a drug sensitivity detection module for performing a drug contact experiment and drug sensitivity detection on the organoid, and a transfer system, wherein a workbench for placing experiment consumables is arranged on the rack; the transfer system is used for conveying a to-be-treated sample tissue into the pretreatment module, conveying a prepared cell suspension into the automatic culture module, conveying an organoid obtained by the automatic culture module into the workbench, and conveying the organoid on the workbench to the drug sensitivity detection module for corresponding drug sensitivity detection; according to the utility model, the problem that the existing culture scheme and drug sensitivity detection are more dependent on manual operation instead of automation can be effectively solved, the manual operation can be replaced, the working efficiency can be improved, and the error rate can be reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biological medicine technology especially, relate to a kind of organoid culture and the automation workstation of drug sensitivity detection. BACKGROUND

[0002] Organoid, namely in vitro culture, self-assembled micro three-dimensional structure, the structure is highly similar to the structure of in-vivo tissue organ, contains various cell types, and can be self-renewed, simulates the part function of in-vivo tissue organ.Organoid as a new biological model, with unique simulation performance, has great potential in disease mechanism research, drug screening, regenerative medicine, biological material evaluation and other aspects.In recent years, with the continuous deepening of related research, organoid technology develops very rapidly, and also provides more possibilities for the treatment of tumor patients, at present, organoid technology remains in laboratory stage, and in the process of medical transformation, the production of organoid needs to be standardized and standardized, and the best form of standardization is automation.At present, more culture programs and drug sensitivity detection rely on manual operation rather than automation, but manual operation has great limitations, not only needs to invest a lot of learning and training cost, and manual experiment operation is prone to error, leading to phenotype has high variability, which is more obvious in drug sensitivity detection process, and even may cover the results of drug screening, cannot achieve the expected results, how to stably and reliably carry out organoid production and drug sensitivity detection has become a problem to be solved in the field. CONTENT OF UTILITY MODEL

[0003] The utility model aims at the deficiency existing in prior art and provides a kind of automation workstation of organoid culture and drug sensitivity detection, can effectively solve the problem that more culture programs and drug sensitivity detection rely on manual operation rather than automation, not only can replace manual work, but also can improve work efficiency and reduce error rate.

[0004] To achieve the above object, the utility model provides a kind of automation workstation of organoid culture and drug sensitivity detection, including rack, the pre-treatment module of sample tissue into cell suspension, the automatic culture module of cell suspension into organoid, the drug sensitivity detection module of drug contact experiment and drug sensitivity detection to organoid, transfer system, rack is provided with the workbench for placing experimental consumables;The transfer system is used to send the sample tissue to be handled into pre-treatment module, and the prepared cell suspension is sent into automatic culture module, then the organoid obtained by automatic culture module is sent into workbench, and then the organoid on workbench is sent to drug sensitivity detection module to carry out corresponding drug sensitivity detection.

[0005] Further improvement of the above scheme is that the transfer system comprises a pipette transfer device, a microplate clamping transfer device for clamping microplates, and a pipetting device for pipetting and transferring reagent solutions, the pipette transfer device is used to clamp and transfer reagent tubes and / or open the caps between the pretreatment module and the automated culture module, the microplate clamping transfer device is used to clamp microplates between the drug sensitivity detection module, the automated culture module and the workbench, and the pipetting device is used to complete pipetting operations between the automated culture module, the drug sensitivity detection module and the workbench.

[0006] Further improvement of the above scheme is that the pipette transfer device comprises an XYZ-axis motion driving assembly arranged on a rack, a pipette assembly support seat, and a pipette assembly rotatably arranged on the pipette assembly support seat, the XYZ-axis motion driving assembly is drivingly connected to the pipette assembly support seat; the pipette assembly comprises a rotation driving assembly rotatably arranged on the pipette assembly support seat, a rotation seat connected to the power output end of the rotation driving assembly, a pipette driving assembly arranged on the rotation seat, and a pipette jaw slidingly connected to the rotation seat in the horizontal direction, the pipette driving assembly is drivingly connected to the pipette jaw, and an anti-skid pattern is further arranged on the pipette jaw.

[0007] Further improvement of the above scheme is that the microplate clamping transfer device comprises a multi-axis microplate clamping manipulator assembly, the multi-axis microplate clamping manipulator assembly comprises an X-axis sliding rail arranged on a rack, an X-axis driving assembly, a Y-axis sliding support seat slidingly arranged on the X-axis sliding rail in the X-axis direction, a Y-axis driving assembly, and a microplate clamping hand assembly slidingly arranged on the Y-axis sliding support seat in the Y-axis direction, the X-axis driving assembly is drivingly connected to the Y-axis sliding support seat, and the Y-axis driving assembly is drivingly connected to the microplate clamping hand assembly; the microplate clamping hand assembly comprises a microplate clamping hand support seat, a microplate clamping hand sliding seat slidingly arranged on the microplate clamping hand support seat in the Y-axis direction, a microplate clamping hand sliding driving assembly arranged on the microplate clamping hand support seat, a microplate clamping hand rotation seat rotatably arranged on the microplate clamping hand sliding seat in the horizontal direction, a microplate clamping hand rotation driving assembly arranged on the microplate clamping hand sliding seat, two L-shaped clamping jaws slidingly arranged on the microplate clamping hand sliding seat in the X-axis direction, and an L-shaped clamping jaw driving assembly arranged on the microplate clamping hand rotation seat, the microplate clamping hand sliding driving assembly is drivingly connected to the microplate clamping hand sliding seat, the microplate clamping hand rotation driving assembly is drivingly connected to the microplate clamping hand rotation seat, and the L-shaped clamping jaw driving assembly is drivingly connected to the L-shaped clamping jaw.

[0008] Further improvement of the above scheme is that the clamp plate transferring device further comprises a rotating plate transferring assembly arranged on the top of the rack, the rotating plate transferring assembly comprises a rotating plate transferring sliding rail arranged on the rack, a rotating plate transferring sliding seat slidably connected to the rotating plate transferring sliding rail, a rotating plate transferring sliding driving assembly arranged on the rotating plate transferring sliding rail, a Z-axis sliding arm slidably connected to the rotating plate transferring sliding rail along the Z-axis direction, a Z-axis sliding arm driving assembly arranged on the rotating plate transferring sliding seat, a rotating plate supporting hand assembly rotatably connected to the bottom of the Z-axis sliding arm, and a rotating plate supporting hand rotating driving assembly arranged on the Z-axis sliding arm, the rotating plate transferring sliding driving assembly is drivingly connected to the rotating plate transferring sliding seat, the Z-axis sliding arm driving assembly is drivingly connected to the Z-axis sliding arm, and the rotating plate supporting hand rotating driving assembly drives the rotating plate supporting hand assembly to rotate.

[0009] Further improvement of the above scheme is that the pipetting device comprises a pipetting manipulator sliding rail arranged on the rack along the X-axis direction, a pipetting manipulator sliding seat slidably arranged on the pipetting manipulator sliding rail along the X-axis direction, a pipetting manipulator support seat arranged on the pipetting manipulator sliding seat, a pipette slidably arranged on the pipetting support seat along the Y-axis direction, and a pipetting manipulator driving assembly arranged on the pipetting manipulator support seat, the pipetting manipulator driving assembly is drivingly connected to the pipette, and the pipette is provided with a plurality of pipetting heads.

[0010] Further improvement of the above scheme is that the system further comprises a camera and a cell D imager, the pretreatment module comprises a tissue treatment instrument, a sample storage box, a centrifuge and a cell counter arranged on the rack, the automated culture module comprises a carbon dioxide incubator, the drug sensitivity detection module comprises a freezer and a chemiluminescence instrument, and the transferring system comprises a pinch pipe transferring device, a clamp plate transferring device and a pipetting device, and the clamp plate transferring device comprises a multi-axis clamp plate manipulator assembly and a rotating plate transferring assembly.

[0011] Further improvement of the above scheme is that the sample storage box, the tissue treatment instrument, the centrifuge, the workbench and the chemiluminescence instrument are arranged on the second layer of the rack, the cell counter is arranged on the first layer of the rack, the freezer and the carbon dioxide incubator are arranged at the back of the rack, the camera is arranged at the back of the tissue treatment instrument, and the multi-axis clamp plate manipulator assembly is arranged on the rear end face of the rack

[0012] Further improvement of the above scheme is that the sample storage box is arranged on the left side of the second layer of the rack, the sample storage box is arranged on the first side of the second layer of the rack, and the tissue processing instrument, the centrifuge, the workbench and the chemiluminescence instrument are sequentially arranged on the second layer of the rack along the process direction from the sample storage box.

[0013] Further improvement of the above scheme is that the pipetting device is provided with two, and the pinch pipe transfer device, the pipetting device, the transfer plate transfer assembly and the pipetting device are sequentially arranged along the process direction above the second layer of the rack.

[0014] Further improvement of the above scheme is that the carbon dioxide incubator is further provided with a rotating stack module capable of storing micro-hole plates, the rotating stack module comprises a stack base, a rotating platform rotatably arranged on the stack base, a rotating drive assembly arranged on the stack base, and a storage rack arranged on the rotating platform for storing materials, and the rotating drive assembly is drivingly connected with the rotating platform.

[0015] Further improvement of the above scheme is that the carbon dioxide incubator is further provided with a rotating stack module capable of storing micro-hole plates, the rotating stack module comprises a stack base, a rotating platform rotatably arranged on the stack base, a rotating drive assembly arranged on the stack base, and a storage rack arranged on the rotating platform for storing materials, and the rotating drive assembly is drivingly connected with the rotating platform.

[0016] Further improvement of the above scheme is that the rotating stack module further comprises a conveying mechanism for completing transportation of materials in different directions and a feeding mechanism for feeding materials to the conveying mechanism; the conveying mechanism comprises a support frame arranged on the stack base, a Z-axis drive assembly arranged on the support frame, a first sliding seat slidingly connected with the Z-axis drive assembly in the Z-axis direction, a conveying assembly arranged on the first sliding seat, and the Z-axis drive assembly is drivingly connected with the first sliding seat; and the feeding mechanism comprises a feeding drive assembly arranged on the outer side of the edge of the stack base, a second sliding seat slidingly connected with the feeding drive assembly in the Y-axis direction, and a material placing table arranged on the second sliding seat, and the feeding drive assembly is drivingly connected with the second sliding seat.

[0017] The utility model has the following beneficial effects:

[0018] 1: The utility model uses the transfer system to cooperate with the pretreatment module, the automation culture module, the drug sensitive detection module, replaces the manual operation and completes a series of processes between the organoid culture and the drug sensitive detection, realizes the automation control completely, thereby reduces the difference existing in the organoid culture process, improves precision, stability and repeatability greatly, reduces the artificial cost also simultaneously and can guarantee the high consistency and repeatability of the organoid, avoids the error brought by manual work, improves the drug sensitive detection work efficiency effectively.

[0019] 2: The utility model discloses a traditional manual experiment or the organoid workstation of present, and the organoid culture process is as a whole production line, and single equipment can only complete single experiment process, in the culture to drug sensitive detection process, can only wait for the next experiment step to continue the next experiment step, not only low work efficiency and not applicable to the work demand of large batch, and the automation workstation provided by the utility model splits the organoid from culture to drug sensitive detection into multiple process modules, and cooperates through the transfer system between them, and the transfer system completes the pipetting and clamping operation, when completing the large batch operation, can process a large number of samples simultaneously, need not wait for the previous process to be completed, and the idle module can alternate to complete the operation, can effectively be applicable to the large batch operation, not only improves the work efficiency, and replaces the manual operation to guarantee the stability. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 It is the structure schematic diagram of the utility model.

[0021] Figure 2 It is the structure schematic diagram of the utility model's pipe clamp transfer device.

[0022] Figure 3 It is the structure schematic diagram of the utility model's multi-axis clamp plate mechanical hand assembly.

[0023] Figure 4 It is the structure schematic diagram of the utility model's transfer plate transfer assembly.

[0024] Figure 5 It is the structure schematic diagram of the utility model's pipetting device.

[0025] Figure 6 It is the structure schematic diagram of the stack module in the carbon dioxide incubator of the utility model.

[0026] Figure 7 It is the structure schematic diagram of another embodiment of the utility model.

[0027] Figure 8 It is the comparison diagram of the utility model's workstation and the batch difference of manual experiment.

[0028] Figure 9The utility model discloses organ cell extraction cell acquisition rate and artificial processing contrast chart.

[0029] Mark explanation: frame 1, tissue processing instrument 11, sample storage box 12, centrifuge 13, cell counter 14, freezer 15, chemiluminescence instrument 16, workbench 17, pretreatment module 18, automatic culture module 19, drug sensitivity detection module 20, transfer system 201, pinch pipe transfer device 2, XYZ axis movement drive assembly 21, pinch pipe assembly support seat 22, pinch pipe assembly 23, rotation drive assembly 231, rotation seat 232, pinch pipe drive assembly 233, pinch pipe claw 234, anti -slip line 235, clamp plate transfer device 3, multi -shaft clamp plate manipulator assembly 31, X -axis sliding rail 311, X -axis drive assembly 312, Y -axis sliding support seat 313, Y -axis drive assembly 314, clamp plate support hand assembly 315, clamp plate support hand support seat 3151, clamp plate support hand sliding seat 3152, clamp plate support hand sliding drive assembly 3153, clamp plate support hand rotation seat 3154, clamp plate support hand rotation drive assembly 3155, L -shaped clamp jaw 3156, L -shaped clamp jaw drive assembly 3157, rotation plate transfer assembly 32, rotation plate transfer sliding rail 321, rotation plate transfer sliding seat 322, rotation plate transfer sliding drive assembly 323, Z -axis sliding arm 324, Z -axis sliding arm drive assembly 325, rotation plate support hand rotation drive assembly 326, rotation plate support hand assembly 327, rotation plate clamp jaw support seat 3271, rotation plate clamp jaw 3272, rotation plate clamp jaw drive assembly 3273, pipetting device 4, pipetting manipulator sliding rail 41, pipetting manipulator sliding seat 42, pipetting manipulator support seat 43, pipette 44, pipetting head 441, pipetting manipulator drive assembly 45, carbon dioxide incubator 5, rotary stack module 51, stack base 511, rotary platform 512, rotary drive assembly 513, storage rack 514, conveying mechanism 52, support frame 521, Z -axis drive assembly 522, first sliding seat 523, conveying assembly 524, feeding mechanism 53, feeding drive assembly 531, second sliding seat 532, material placing table 533, camera 6, cell 3D imager 7, code scanning assembly 8, code assigning assembly 9. DETAILED DESCRIPTION

[0030] The utility model will be further explained in connection with the drawings, such as Figures 1-9As shown, the utility model is a kind of organoid culture and drug sensitivity detection's automation workstation, including rack 1, pretreatment module 18, automation culture module 19, drug sensitivity detection module 20, transfer system 201, rack 1 is provided with the workbench 17 for placing experimental consumables;The sample tissue to be handled is sent into pretreatment module 18 by transfer system 201, and the corresponding cell suspension is prepared by pretreatment module 18 from sample tissue, and the cell suspension prepared is sent into automation culture module 19 by transfer system 201 and is cultured to obtain organoid, and the obtained organoid is sent into workbench 17 by transfer system 201, and the organoid on workbench 17 is sent to drug sensitivity detection module 20 by transfer system 201 to carry out corresponding drug contact experiment, drug sensitivity detection.

[0031] The utility model transfer system 201 includes pipe clamp transfer device 2, clamp plate transfer device 3 for clamping microwell plate, pipette device 4 for sucking and transferring reagent solution, pipe clamp transfer device 2 is used to complete reagent tube clamping and / or uncapping operation between pretreatment module 18 and automation culture module 19, clamp plate transfer device 3 is used to complete the operation of clamping microwell plate between drug sensitivity detection module 20, automation culture module 19 and workbench 17, pipette device 4 is used to complete pipetting operation between automation culture module 19, drug sensitivity detection module 20 and workbench 17;Compared with prior organoid workstation, only simple stacking of processing device or system is carried out, and still need to be operated by staff, and sudden situation of processing equipment or system cannot be realized True sense of automation, and the utility model transfer system 201 is divided into pipe clamp transfer device 2, clamp plate transfer device 3 and pipette device 4, can complete a series of manual operations such as organoid culture to drug sensitivity detection internal transfer microwell plate, transfer reagent tube, pipetting, drop liquid, replace all manual operations that equipment in organoid workstation cannot complete, cooperate with the equipment module of the workstation, and can completely realize the complete automation of organoid from culture to drug sensitivity detection process;As shown below:

[0032] Organoid experimental workflow Average time required for automation (hours / case) Average time required for manual (hours / case) Sample pre-treatment 3.0 6.0 Media change 0.1 0.2 Quality control: AI image evaluation / manual measurement 0.1 0.4 Drug sensitivity plating 2.0 4.0 Drug sensitivity addition 0.3 1.0 Data review calculation 0.2 1.0 Data analysis, report issuance 0.3 1.0 Overall workflow 6.0 13.6

[0033] The utility model workstation different device division of labor cooperation module in workstation, working time is significantly shortened, can efficiently, quickly complete a series of work of organoid from culture to drug sensitivity detection, effectively avoid high failure rate and instability of manual operation, as shown below:

[0034] Sample number Automated Z-value Manual Z-value 0112101643 0.638672 0.622292 0112101808 0.76021 0.610336 0112101849 0.815608 0.700811 0112101964 0.778498 0.573141 0112101977 0.883144 0.738276 0112102015 0.573906 0.597906 0112102021 0.598051 0.429114 0112102030 0.812103 0.464789 0112102047 0.751359 0.749469 0112102052 0.718839 0.665776 0112102068 0.655512 0.686933 0112102089 0.899502 0.930788 0112102116 0.681686 0.785486 0112102141 0.847088 0.854693 0112102150 0.684615 0.768221 0112102151 0.613971 0.592601 0112102154 0.779722 0.524565 0112102155 0.780431 0.639858 0112102163 0.647895 0.759628 0112102165 0.489311 0.494593

[0035] Preparation 20 cases of handover drug sensitive sample, each sample manual and automation each 3 group of drugs, 3 complex holes in each group of drugs, after drug sensitivity detection, 40 cases of sample Z value is calculated, Z value is greater than or equal to 0.5, namely qualified, the workstation automation stability and manual stability of the utility model are calculated, as can be seen from the figure, the automation stability is 19 / 20=95%, the manual stability is 17 / 20=85%, the different device of the utility model is not affected, single device failure will not affect the work of another device, improve the work efficiency at the same time, ensure the stability, compared with the low efficiency, low stability manual operation experiment, further applicable to large quantities, large output batch organ culture and drug sensitive detection work occasion.

[0036] The pipe clamping and transferring device 2 comprises an XYZ axis movement driving assembly 21 arranged on the rack 1, a pipe clamping assembly support seat 22, and a pipe clamping assembly 23 rotatably arranged on the support seat, the XYZ axis movement driving assembly 21 is drivingly connected with the pipe clamping assembly support seat 22; the pipe clamping assembly 23 comprises a rotary driving assembly 231 rotatably arranged on the support seat, a rotary seat 232 rotatably connected with a power output end of the rotary driving assembly 231, a pipe clamping driving assembly 233 arranged on the rotary seat 232, and a pipe clamping claw 234 slidingly connected with the rotary seat 232 in the horizontal direction, the pipe clamping driving assembly 233 is drivingly connected with the pipe clamping claw 234, and an antiskid pattern 235 is further arranged on the pipe clamping claw 234; the pipe clamping and transferring device 2 can complete the clamping and transferring operation of the reagent pipe between modules, the antiskid pattern 235 is arranged on the pipe clamping claw 234, compared with the ordinary mechanical hand, the arrangement of the antiskid pattern 235 can ensure that the clamped reagent pipe will not slip off, and can also complete the opening and closing operation of the reagent pipe, and can be better applied to the organ related work flow.

[0037] The utility model clamping plate transfer device 3 includes multiaxis clamping plate manipulator subassembly 31, multiaxis clamping plate manipulator subassembly 31 includes setting up on the X axle slide rail 311 of frame 1, X axle drive subassembly 312, along the Y axle sliding support seat 313 of X axle direction sliding setting on the X axle slide rail 311, Y axle drive subassembly 314, along the clamping plate hand support subassembly 315 of Y axle direction sliding setting on the Y axle sliding support seat 313, X axle drive subassembly 312 drive connection Y axle sliding support seat 313, Y axle drive subassembly 314 drive connection clamping plate hand support subassembly 315, clamping plate hand support subassembly 315 includes clamping plate hand support seat 3151, along the clamping plate hand support sliding seat 3152 of Y axle direction sliding setting on clamping plate hand support seat 3151, setting on clamping plate hand support seat 3151 clamping plate hand support sliding drive subassembly 3153, along the clamping plate hand support rotating seat 3154 of horizontal direction rotation setting on clamping plate hand support sliding seat 3152, setting on clamping plate hand support sliding seat 3152 clamping plate hand support rotating drive subassembly 3155, two along X axle direction opposite sliding setting on clamping plate hand support sliding seat 3152 L shape clamping jaw 3156, setting on clamping plate hand support rotating seat 3154 L shape clamping jaw drive subassembly 3157, clamping plate hand support sliding drive subassembly 3153 drive connection clamping plate hand support sliding seat 3152, clamping plate hand support rotating drive subassembly 3155 drive connection clamping plate hand support rotating seat 3154, L shape clamping jaw drive subassembly 3157 drive connection L shape clamping jaw 3156, L shape clamping jaw 3156 can hold up micro -hole plate also can with opposite L shape clamping jaw 3156 receive thus clamping micro -hole plate, complete each module micro -hole plate or the transportation of board.

[0038] The utility model discloses clamp plate transfer device 3 still include setting in the top of rack rotation plate transfer subassembly 32, rotation plate transfer subassembly 32 includes setting on the rotation plate transfer slide rail 321 of rack, the rotation plate transfer sliding seat 322 of sliding connection on rotation plate transfer slide rail 321, setting on rotation plate transfer slide rail 321 rotation plate transfer sliding drive subassembly 323, along Z axle direction sliding connection on rotation plate transfer slide rail 321 Z axle sliding arm 324, setting on rotation plate transfer sliding seat 322 Z axle sliding arm drive subassembly 325, rotationally connected in Z axle sliding arm 324 bottom rotation plate handle subassembly 327, setting on Z axle sliding arm 324 rotation plate handle rotation drive subassembly 326, rotation plate transfer sliding drive subassembly 323 drive connection rotation plate transfer sliding seat 322, Z axle sliding arm drive subassembly 325 drive connection Z axle sliding arm 324, rotation plate handle rotation drive subassembly 326 drive rotation plate handle subassembly 327 rotation, rotation plate handle subassembly 327 includes setting in Z axle sliding arm 324 bottom rotation plate clamp jaw support seat 3271, two along X axle direction opposite sliding setting on rotation plate clamp jaw support seat 3271 rotation plate clamp jaw 3272, setting on rotation plate clamp jaw support seat 3271 rotation plate clamp jaw drive subassembly 3273, rotation plate clamp jaw drive subassembly 3273 drive two rotation plate clamp jaw 3272 along X axle direction relative motion, and rotation plate transfer subassembly 32 can complete the work of rack's transfer micro -hole plate, can cooperate with multi -shaft clamp plate mechanical hand subassembly 31 work, complete each module between transport micro -hole plate operation, and when multi -shaft clamp plate mechanical hand subassembly 31 busy can complete the operation of the transport micro -hole plate to be completed, compared with traditional single mechanical arm complete work, the utility model rotation plate transfer subassembly's setting, not only can complete the work that multi -shaft clamp plate mechanical hand subassembly 31 cannot complete when busy, and can replace work when multi -shaft clamp plate mechanical hand subassembly 31 fails, that is, improve work efficiency, also improve the stability of the utility model workstation.

[0039] The utility model discloses pipette device 4 includes setting in the rack 1 on pipette mechanical hand slide rail 41 along X axle direction, sliding setting on pipette mechanical hand slide rail 41 along X axle direction pipette mechanical hand sliding seat 42, setting on pipette mechanical hand sliding seat 42 pipette mechanical hand support 43, along Y axle direction sliding setting on pipette support seat pipette 44, setting on pipette mechanical hand support 43 pipette mechanical hand drive subassembly 45, pipette mechanical hand drive subassembly 45 drive connection pipette 44, and pipette 44 is provided with a plurality of pipette head 441, and pipette mechanical hand cooperation pipette 44 can complete the operation that needs to transfer solution, reagent in the process of organoid culture to drug sensitivity detection, and pipette 44 is provided with a plurality of pipette head 441, and simultaneously completes a plurality of pipette operations, effectively promotes work efficiency, and can be used for pipetting different reagents, avoids mutual cross infection, and does not need to change pipette head 141, such as Figure 9As shown, 10 tissue samples are prepared, each sample is divided into two equal parts, and artificial digestion treatment and automatic sample treatment are carried out respectively, and the cell harvest amount of 10 samples is counted, after 7 days of culture, the average value of the number of organoids and the diameter of the organoids is detected, and it can be seen that, compared with the traditional worker operating a pipette, the workstation cooperates with the module device to work, which can be better applied to the work occasions such as organoid culture, drug sensitivity detection and other work occasions which need to frequently carry out pipetting and liquid changing.

[0040] The preprocessing module 18 of the utility model includes tissue processing instrument 11, sample storage box 12, centrifuge 13, cell counter 14 arranged on rack 1, tissue processing instrument 11 is used to cut and digest tissue sample, centrifuge 13 is used to filter tissue, cell counter 14 is used to detect cell quantity, and sample storage box 12 is used to temporarily store sample tissue;Wherein tissue processing instrument 11 is arranged on the upper layer of rack 1, centrifuge 13 is arranged on the side of tissue processing instrument 11, and the single cell suspension corresponding to the tissue sample treated by preprocessing module 18 is prepared by being placed in tissue processing instrument 11 and centrifuge 13 by transfer system 201, and cell counting is carried out through cell counter 14 to reach the required cell concentration.

[0041] The automatic culture module 19 of the utility model includes carbon dioxide incubator 5, and carbon dioxide incubator 5 is used to provide suitable culture environment, and of course carbon dioxide incubator 5 can also be provided with temperature control module and carbon dioxide concentration control module to adjust the temperature and carbon dioxide concentration in the box.

[0042] The organoid culture and drug sensitivity detection automatic workstation provided by the utility model further includes camera 6 and cell 3D imager 7, wherein preprocessing module 18 includes tissue processing instrument 11, sample storage box 12, centrifuge 13 and cell counter 14 arranged on rack 1, automatic culture module 19 includes carbon dioxide incubator 5, drug sensitivity detection module 20 includes freezer 15 and chemiluminescence instrument 16, and transfer system 201 includes pinch pipe transfer device 2, clamp plate transfer device 3 and pipetting device 4, and clamp plate transfer device 3 includes multi-axis clamp plate mechanical hand assembly 31 and rotating plate transfer assembly 32.

[0043] In some ways, the sample storage box 12, the tissue processing instrument 11, the centrifuge 13, the workbench 17, the chemiluminescence instrument 16 are all arranged on the second layer of the rack 1, the cell counter 14 is arranged on the first layer of the rack 1, the freezer 15 and the carbon dioxide incubator 5 are arranged at the back of the rack 1, the camera 6 is arranged at the back of the tissue processing instrument 11, the multi-axis clamp plate mechanical hand assembly 31 is arranged at the back end of the rack 1, the clamp tube transfer device 2, the pipette device 4 and the rotating plate transfer assembly 32 are arranged above the second layer of the rack, the rack is designed as double layers, the structure is more compact, the organoid drug sensitivity detection process can be used to arrange the pretreatment module 18 on the left side of the second layer of the rack 1, the clamp tube transfer device 2 and the pipette device 4 are also arranged above the left side of the second layer of the rack, the workbench 17 and the chemiluminescence instrument are arranged on the right side of the second layer of the rack 1, the rotating plate transfer assembly 31 and another pipette device 4 are arranged above the right side of the second layer of the rack 1, each mechanical hand cooperates and is arranged above the module needing to use the corresponding mechanical hand device, the working stroke of the mechanical hand device is short, the efficiency is improved, the equipment integration is high, the multi-axis clamp plate mechanical hand assembly 31 is arranged at the back of the rack 1, can complete the transfer of the first layer and the second layer of the rack 1, the work of the clamp plate, not only this, the multi-axis clamp plate mechanical shaft assembly 31 can be moved to the position of each module to complete the transfer and the work of the clamp plate, can also replace the rotating plate transfer assembly 32 to complete the transfer and the work of the clamp plate when idle, further improve the work efficiency, the freezer 15 and the carbon dioxide incubator 5 are arranged at the back of the rack 1, reduce the space occupation, and facilitate the multi-axis clamp plate mechanical hand assembly 31 to take out the consumables from the freezer 15 and complete the clamping and transferring operation of the culture dish with the carbon dioxide incubator 5; the structure of each module of the workstation is compact, and the modules are sequentially arranged or arranged adjacent to the position on the rack 1 according to the drug sensitivity detection process, the integration is higher, and the transfer system 201 is divided into different devices and cooperates to be arranged in the corresponding module working area, cooperates with each module in the workstation, can efficiently and quickly complete a series of work from culturing to drug sensitivity detection of the organoid, and different devices work independently, and a single device failure will not affect the work of another device, such as Figure 9 As shown in the figure, 10 organoids are prepared, and two independent organoid drug sensitivity detections are respectively carried out by automatic equipment and manual operation. The comparative detection results show that 9 samples with statistical results greater than 0.05 are obtained by comparing the batch difference of the automatic equipment detection results; 8 samples with statistical results greater than 0.05 are obtained by comparing the batch difference of the manual operation detection results. The difference between the multiple batches of the organoid workstation is smaller than that of the manual operation, which ensures the stability of the multiple batch sample processing. The arrangement order of the equipment mentioned in the utility model is not unique, the embodiment only provides a preferred implementation manner, and different equipment arrangements can be made according to different experimental processes, which can be understood as equivalent replacement of the embodiment of the utility model.

[0044] In other modes, the sample storage box 12 is arranged on the left side of the second layer of the rack 1, the tissue treatment instrument 11 is arranged on the right side of the sample storage box 12, the centrifuge 13 is arranged on the right side of the tissue treatment instrument 11, the workbench 17 is arranged on the right side of the centrifuge 13, the chemiluminescence instrument 16 is arranged on the right side of the workbench 17, and the cell counter 14 is arranged on the left side of the first layer of the rack 1. In this mode, the core links of organoid culture and drug sensitivity detection are arranged together, the internal space of the workstation is effectively utilized, the sample transmission distance between each process is greatly shortened, the pollution and transmission accident probability are reduced, the core device is arranged in the middle position which is easy to observe by the experimenter, and the whole culture and detection process is monitored by the experimenter to find problems in time. In addition, the auxiliary equipment and large volume equipment arranged in the lower part and the rear part are more conducive to the addition and maintenance of experimental consumables and auxiliary preparations, balance the center of gravity of the equipment, and facilitate installation and debugging.

[0045] As shown in the drawings, Figure 7 Figure 8 In other embodiments of the present application, the chemiluminescence instrument 16 is arranged on the left side of the first layer of the rack 1, which is different from the above-mentioned embodiments. Of course, different devices can be placed according to different experimental processes. The device placement order of the present application is not limited to this, and other reasonable position placement orders can be understood as equivalent replacements of the embodiments of the present application.

[0046] In other modes, two pipetting devices 4 are arranged, and the pinch pipe transfer device 2, the pipetting device 4, the transfer plate assembly 32 and the pipetting device 4 are sequentially arranged from left to right above the second layer of the rack 1. One pipetting device 4 is responsible for pretreatment culture, and the other is responsible for drug sensitivity detection processing, which improves work efficiency and avoids mixing of pipetting heads to affect experimental results. Sequentially placed can further work in the position of each module, reduce the working stroke of the corresponding device, optimize the working mode of the mechanical hand, thereby improving the work efficiency. Of course, the pipetting device 4 of the present application can also be arranged in other quantities according to specific needs, and two is only a preferred embodiment of the present application. The device placement position of the present application can be adjusted in different directions according to specific experimental processes, which can be linearly placed or nonlinearly placed, and can be understood as equivalent replacements of the embodiments of the present application.

[0047] The utility model discloses carbon dioxide incubator 5 still be provided with the rotatory stack module 51 of the micro -hole board of storage, rotatory stack module 51 includes stack base 511, rotates and set on the rotatory platform 512 of stack base 511, set on the rotatory drive assembly 513 of stack base 511, set on the rotatory platform 512 for the storage rack 514 of depositing material, rotatory drive assembly 513 drive connection rotatory platform 512;Rotatory stack module 51 rotatable work, rotatory platform 512 can be equipped with a plurality of storage rack 514, and the micro -hole board or petri dish is stored in the partition of storage rack 514, and the stack efficiency is high, and the stack capacity is big.

[0048] The utility model discloses rotatory stack module 51 still includes the transport mechanism 52 for completing different orientation micro -hole board transportation, the feeding mechanism 53 for giving transport mechanism 52 to transport micro -hole board;Transport mechanism 52 includes setting on the support frame 521 of stack base 511, set on the Z axle drive assembly 522 of support frame 521, along Z axle direction sliding connection on the first sliding seat 523 of Z axle drive assembly 522, set on the transport assembly 524 of first sliding seat 523, and Z axle drive assembly 522 drive connection first sliding seat 523;Feeding mechanism 53 includes setting on the feeding drive assembly 531 of stack base 511 edge outside, along Y axle direction sliding connection on the second sliding seat 532 of feeding drive assembly 531, set on the material loading turntable 533 of second sliding seat 532, and feeding drive assembly 531 drive connection second sliding seat 532;Transport mechanism 52 and feeding mechanism 53 cooperate and work, and the micro -hole board is sent into the feeding mechanism 53 in incubator in the system of moving 201, and transport mechanism 52 cooperates rotatory platform 512 and divides the storage of micro -hole board, and the working efficiency is high.

[0049] The utility model discloses drug sensitivity detection module 20 includes freezer 15, chemiluminescence instrument 16, and freezer 15 is used to store various need to freeze and preserve reagent, sample, micro -hole board, and chemiluminescence instrument 16 is used for detecting the ATP of cell;Freezer 15 sets up at one side of rack 1, is used to deposit need to freeze and preserve reagent, reagent, equipment, need to move system 201 to take out from freezer 15 in need, and chemiluminescence instrument 16 is used for detecting the ATP in cell, and the embodiment preferably is enzyme mark appearance, and before operation, move system 201 first adds the light-emitting detection reagent in the micro -hole board of detection, and is detected in enzyme mark appearance through move system 201 to judge the effect of drug.

[0050] The utility model also includes process tracking management module, process tracking management module includes code assignment component 9, code scanning component 8, code assignment component 9 is used to stick the identification label of corresponding process stage to corresponding reagent tube or microplate, and code scanning component 8 is used to identify bar code label;Code assignment component 9 sticks identification label, and code scanning component 8 identifies label, which facilitates identifying which process the microplate is in, facilitates management, avoids the removal error of removal system 201 from affecting the whole culture and drug sensitivity detection process, and guarantees the accuracy and stability of the experiment.

[0051] The utility model also includes setting up on the camera 6 of rack 1, cell 3D imaging appearance 7, camera 6 is used for shooting cell photo to observe cell morphology, and cell 3D imaging appearance 7 and camera 6 are all set up on rack 1 and complete cell imaging with removal system 201 to judge whether the organoid of culture reaches the culture standard and obtain the state of organoid after dosing to carry out result analysis.

[0052] Working principle: the utility model first passes through the preprocessing module 18 and removal system 201 cooperation, and the tissue processing appearance 11 is used with centrifuge 13, and the tissue sample is washed, filtered, digested to obtain cell suspension, through the automatic culture module 19, after counting through cell counter 14, through removal system 201 and add carbon dioxide incubator 5 to culture, through camera 6, cell 3D imaging appearance 7 can distinguish whether to complete the culture, through drug sensitivity detection module 20, pipette device 4 and pinch pipe removal and clamp plate removal device 3 complete dosing, and respectively set up control group, and chemical luminescence appearance 16 discriminates drug action, and completes record. The whole experiment process uses removal system 201 and cooperates preprocessing module 18, automatic culture module 19, drug sensitivity detection module 20, replaces manual operation and completes a series of processes between organoid culture and drug sensitivity detection, and realizes automatic control completely, so that the difference existing in the process of organoid culture can be reduced, the precision, stability and repeatability are greatly improved. Meanwhile, the organoid workstation can realize mass production of organoids, reduce labor costs, ensure the yield of organoids, and ensure the high consistency and repeatability of organoids, avoid the error caused by manual operation;In addition, through the cooperation of process tracking management module and drug sensitivity detection module 20, automatic drug contact and automatic drug detection are realized, the accuracy and efficiency of drug detection are guaranteed, and the work efficiency is effectively improved.

[0053] The working method of the organoid culture and drug sensitivity detection workstation according to the embodiment of the application can include the following steps:

[0054] S1: the reagent tube stored in the freezer 15 is taken out by the pinch pipe removal device 2 and placed on the rack 1 for thawing;

[0055] S2: Put the cleaned tissue sample into the reagent tube, and use the pinch tube transfer device 2 in cooperation with the clamp plate transfer device 3 to add the reagent tube with the tissue sample into the tissue processor 11. After the digestion is completed, use the pipetting device 4 to add the HBSS buffer to terminate the digestion, and use the pinch tube transfer device 2 to add the digested reagent tube into the centrifuge 13 to centrifuge and discard the supernatant. Again, use the pipetting device 4 to add the HBSS buffer into the centrifuge 13 to resuspend the precipitate and obtain the cell precipitate;

[0056] S3: Use the pipetting device 4 to add the culture solution to the reagent tube containing the cell precipitate. Use the pinch tube transfer device 2 in cooperation with the pipetting device 4 to clamp the reagent tube into the cell counter 14 for counting. Then use the pipetting device 4 to add the culture solution to prepare the diluted cell suspension;

[0057] S4: Use the clamp plate transfer device 3 to take out the ice box from the freezer 15 and place it on the rack 1. Use the pipetting device 4 to drop the cell suspension into the culture dish, and use the clamp plate transfer device 3 to place the culture dish in the carbon dioxide incubator 5 for culture to obtain the organoids;

[0058] S5: After the culture period is over, use the clamp plate transfer device 3 to take out the culture dish from the carbon dioxide incubator 5, and use the cell 3D imaging instrument 7 to identify whether the culture is complete. If not, return to S4. If yes, place the gel bottom in the tissue processor 11 for dispersion until the organoids are digested into uniform cell clusters containing 3-5 cells. Then terminate the digestion and place it into the centrifuge 13 through the mechanical clamp to centrifuge and discard the supernatant;

[0059] S6: Use the clamp plate transfer device 3 in cooperation with the pinch tube transfer device 2 to inoculate the cell clusters into the corresponding cancer medium taken out from the freezer 15 and set up the control group. Place the inoculated medium into the carbon dioxide incubator 5 for culture. After the culture is completed, use the pipetting device 4 to add the physiological saline solution and take photos under the microscope for record;

[0060] S7: Take out the culture plate and use the pipetting device 4 to add the drug for drug sensitivity detection. Add the luminescence enhancer and place it into the centrifuge 13 for shaking to promote cell lysis. Wait for the luminescence signal to approach stability, use the chemiluminescence instrument 16 for chemiluminescence detection. After the detection is completed, export the data and record for analysis;

[0061] According to the organoid culture and drug sensitivity detection method provided in the embodiments of the present application, the embodiments of the present application build a multi-module and automated workbench 17 for the collection, processing, detection, sub-packaging of organoids, the culture, amplification, digestion, and drug sensitivity detection of organoids. Through the cooperation between the pretreatment module 18, the automated culture module 19, the drug sensitivity detection module 20, and the transfer system 201, the entire organoid culture and drug sensitivity detection process is managed and arranged through the process tracking management module, ensuring the accuracy and safety of the entire organoid culture and drug sensitivity detection process, thereby ensuring that the experiment is efficiently, accurately, and safely performed, effectively preventing human errors from causing failure, and being conducive to the development of drug screening or omics experiments, and providing an important basis for clinical diagnosis and treatment.

[0062] In the embodiments of the present specification, the "first side" is understood as the left side of the workstation, and the "second side" is understood as the right side of the workstation, which are only described as relative positions and are not limited to the utility model. It should be understood that the described positional relationship can be replaced by an appropriate manner in any one or N embodiments or examples.

[0063] In the embodiments of the present specification, the "first layer" is understood as the lower layer of the workstation, and the "second layer" is understood as the upper layer of the workstation.

[0064] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or N embodiments or examples in a suitable manner. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples without contradiction.

[0065] In addition, the terms "first", "second" are only for description purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "N" is at least two, such as two, three, etc., unless otherwise specifically limited.

[0066] Any processes or methods described in the flow charts or otherwise described herein can be understood as representing code modules, segments, or portions of code which include one or more executable instructions for implementing specific logic functions (or steps) of the processes. The various embodiments of the application can include additional or fewer processes, steps, operations, modules, or portions of code, and the various embodiments of the application can include different arrangements, orders, or combinations of the processes, steps, operations, modules, or portions of code.

[0067] Furthermore, each function module in each embodiment of the present application can be integrated in one processing module, or each module can exist physically, or two or more modules can be integrated in one module. The integrated module can be realized in the form of hardware or in the form of a software function module. When the integrated module is realized in the form of a software function module and sold or used as an independent product, it can also be stored in a computer readable storage medium, which can be a read-only memory, a magnetic disk, or an optical disk, etc.

[0068] Although the embodiments of the present application have been shown and described above, it should be understood by those skilled in the art that the above embodiments are exemplary and cannot be understood as limiting the present application, and those skilled in the art can make changes, modifications, replacements, and variations to the above embodiments within the scope of the present application.

Claims

1. An automated workstation for organoid culturing and drug susceptibility testing, characterized in that: The device comprises a rack (1), a pretreatment module (18) for preparing sample tissues into cell suspension, an automatic culture module (19) for culturing the cell suspension into organoids, a drug sensitivity detection module (20) for performing drug contact experiment and drug sensitivity detection on the organoids, and a transfer system (201); the rack (1) is provided with a workbench (17) for placing experimental consumables; the transfer system is used for transferring sample tissues to be treated into the pretreatment module (18), transferring the prepared cell suspension into the automatic culture module (19), then transferring the organoids obtained by the automatic culture module (19) onto the workbench (17), and then transferring the organoids on the workbench (17) to the drug sensitivity detection module (20) for corresponding drug contact experiment and drug sensitivity detection.

2. An automated workstation for organoid culturing and drug sensitivity testing according to claim 1, characterized in that: The transfer system (201) comprises a pipette clamping transfer device (2), a plate clamping transfer device (3) for clamping microplates, and a pipetting device (4) for pipetting and transferring reagent solutions; the pipette clamping transfer device (2) is used for clamping and transferring reagent tubes and / or opening the caps between the pretreatment module (18) and the automatic culture module (19); the plate clamping transfer device (3) is used for clamping microplates between the drug sensitivity detection module (20), the automatic culture module (19) and the workbench (17); and the pipetting device (4) is used for pipetting between the automatic culture module (19), the drug sensitivity detection module (20) and the workbench (17).

3. An automated workstation for organoid culturing and drug sensitivity testing according to claim 2, characterized in that: The pipette clamping transfer device (2) comprises an XYZ-axis motion driving assembly (21) arranged on the rack (1), a pipette assembly support seat (22), and a pipette assembly (23) rotatably arranged on the pipette assembly support seat (22); the XYZ-axis motion driving assembly (21) is drivingly connected to the pipette assembly support seat (22); the pipette assembly (23) comprises a rotation driving assembly (231) rotatably arranged on the pipette assembly support seat (22), a rotation seat (232) connected to the power output end of the rotation driving assembly (231), a pipette driving assembly (233) arranged on the rotation seat (232), and a pipette claw (234) slidingly connected to the rotation seat (232) in the horizontal direction; the pipette driving assembly (233) is drivingly connected to the pipette claw (234); and the pipette claw (234) is further provided with anti-skid lines (235).

4. The automated workstation for organoid culture and drug susceptibility testing of claim 2, wherein: The clamp plate conveying device (3) comprises a multi-axis clamp plate manipulator assembly (31), the multi-axis clamp plate manipulator assembly (31) comprises an X-axis sliding rail (311) arranged on the rack (1), an X-axis driving assembly (312), a Y-axis sliding support seat (313) arranged on the X-axis sliding rail (311) and sliding in the X-axis direction, a Y-axis driving assembly (314), a clamp plate supporting hand assembly (315) arranged on the Y-axis sliding support seat (313) and sliding in the Y-axis direction, the X-axis driving assembly (312) is drivingly connected with the Y-axis sliding support seat (313), and the Y-axis driving assembly (314) is drivingly connected with the clamp plate supporting hand assembly (315); the clamp plate supporting hand assembly (315) comprises a clamp plate supporting hand support seat (3151), a clamp plate supporting hand sliding seat (3152) arranged on the clamp plate supporting hand support seat (3151) and sliding in the Y-axis direction, a clamp plate supporting hand sliding driving assembly (3153) arranged on the clamp plate supporting hand support seat (3151), a clamp plate supporting hand rotating seat (3154) arranged on the clamp plate supporting hand sliding seat (3152) and rotating in the horizontal direction, a clamp plate supporting hand rotating driving assembly (3155) arranged on the clamp plate supporting hand sliding seat (3152), two L-shaped clamping jaws (3156) arranged on the clamp plate supporting hand sliding seat (3152) and sliding in the X-axis direction, and an L-shaped clamping jaw driving assembly (3157) arranged on the clamp plate supporting hand rotating seat (3154); the clamp plate supporting hand sliding driving assembly (3153) is drivingly connected with the clamp plate supporting hand sliding seat (3152), the clamp plate supporting hand rotating driving assembly (3155) is drivingly connected with the clamp plate supporting hand rotating seat (3154), and the L-shaped clamping jaw driving assembly (3157) is drivingly connected with the L-shaped clamping jaw (3156).

5. An automated workstation for organoid culturing and drug sensitivity testing according to claim 4, characterized in that: The clamp plate transfer device (3) further comprises a rotating plate transfer assembly (32) arranged on the top of the rack, the rotating plate transfer assembly (32) comprises a rotating plate transfer sliding rail (321) arranged on the rack, a rotating plate transfer sliding seat (322) slidingly connected to the rotating plate transfer sliding rail (321), a rotating plate transfer sliding drive assembly (323) arranged on the rotating plate transfer sliding rail (321), a Z-axis sliding arm (324) slidingly connected to the rotating plate transfer sliding rail (321) along the Z-axis direction, a Z-axis sliding arm drive assembly (325) arranged on the rotating plate transfer sliding seat (322), a rotating plate supporting hand assembly (327) rotatably connected to the bottom of the Z-axis sliding arm (324), and a rotating plate supporting hand rotating drive assembly (326) arranged on the Z-axis sliding arm (324), the rotating plate transfer sliding drive assembly (323) is drivingly connected to the rotating plate transfer sliding seat (322), the Z-axis sliding arm drive assembly (325) is drivingly connected to the Z-axis sliding arm (324), and the rotating plate supporting hand rotating drive assembly (326) drives the rotating plate supporting hand assembly (327) to rotate; the rotating plate supporting hand assembly (327) comprises a rotating plate clamp jaw support seat (3271) arranged on the bottom of the Z-axis sliding arm (324), two rotating plate clamp jaws (3272) slidingly arranged on the rotating plate clamp jaw support seat (3271) in the X-axis direction, and a rotating plate clamp jaw drive assembly (3273) arranged on the rotating plate clamp jaw support seat (3271), and the rotating plate clamp jaw drive assembly (3273) drives the two rotating plate clamp jaws (3272) to move in the X-axis direction.

6. An automated workstation for organoid culturing and drug sensitivity testing according to claim 2, characterized in that: The pipetting device (4) comprises a pipetting manipulator sliding rail (41) arranged on the rack (1) in the X-axis direction, a pipetting manipulator sliding seat (42) slidingly arranged on the pipetting manipulator sliding rail (41) in the X-axis direction, a pipetting manipulator support seat (43) arranged on the pipetting manipulator sliding seat (42), a pipette (44) slidingly arranged on the pipetting support seat in the Y-axis direction, and a pipetting manipulator drive assembly (45) arranged on the pipetting manipulator support seat (43), the pipetting manipulator drive assembly (45) is drivingly connected to the pipette (44), and the pipette (44) is provided with a plurality of pipetting heads (441).

7. The automated workstation for organoid culture and drug susceptibility testing of claim 1, wherein: Further comprising a camera (6) and a cell 3D imager (7), the pretreatment module (18) comprises a tissue treatment instrument (11), a sample storage box (12), a centrifuge (13), and a cell counter (14) arranged on the rack (1), the automated culture module (19) comprises a carbon dioxide incubator (5), the drug sensitivity detection module (20) comprises a freezer (15) and a chemiluminescence instrument (16), and the transfer system (201) comprises a clamp tube transfer device (2), a clamp plate transfer device (3), and a pipetting device (4), and the clamp plate transfer device (3) comprises a multi-axis clamp plate manipulator assembly (31) and a rotating plate transfer assembly (32).

8. An automated workstation for organoid culturing and drug sensitivity testing according to claim 7, characterized in that: The sample storage box (12), tissue treatment instrument (11), centrifuge (13), workbench (17), chemiluminescence instrument (16) are arranged on the second layer of the rack (1), the cell counter (14) is arranged on the first layer of the rack (1), the freezer (15) and the carbon dioxide incubator (5) are arranged at the back of the rack (1), the camera (6) is arranged at the back of the tissue treatment instrument (11), and the multi-axis clamping plate mechanical hand assembly (31) is arranged at the back end surface of the rack (1).

9. An automated workstation for organoid culturing and drug sensitivity testing according to claim 7, characterized in that: The carbon dioxide incubator (5) is further provided with a rotating stack module (51) capable of storing well plates, the rotating stack module (51) comprises a stack base (511), a rotating platform (512) rotatably arranged on the stack base (511), a rotating drive assembly (513) arranged on the stack base (511), and a storage rack (514) arranged on the rotating platform (512) for storing materials, and the rotating drive assembly (513) is drivingly connected with the rotating platform (512).

10. An automated workstation for organoid culturing and drug sensitivity testing according to claim 9, characterized in that: The rotating stack module (51) further comprises a conveying mechanism (52) for transporting materials in different directions and a feeding mechanism (53) for feeding materials to the conveying mechanism (52); the conveying mechanism (52) comprises a support frame (521) arranged on the stack base (511), a Z-axis drive assembly (522) arranged on the support frame (521), a first sliding seat (523) slidingly connected with the Z-axis drive assembly (522) in the Z-axis direction, and a conveying assembly (524) arranged on the first sliding seat (523), and the Z-axis drive assembly (522) is drivingly connected with the first sliding seat (523); the feeding mechanism (53) comprises a feeding drive assembly (531) arranged outside the edge of the stack base (511), a second sliding seat (532) slidingly connected with the feeding drive assembly (531) in the Y-axis direction, and a material placing table (533) arranged on the second sliding seat (532), and the feeding drive assembly (531) is drivingly connected with the second sliding seat (532).

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