Device for improving transfer efficiency and safety of embryo culture dish
By using an automated control box lid device in ICSI operations, the cumbersome and safety issues of embryo transfer in culture dishes have been resolved, achieving more efficient and safer oocyte transfer.
Patent Information
- Application Number
- CN202520093311.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-15
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2035-01-15
AI Technical Summary
The current ICSI procedure involves a cumbersome process of transferring embryo culture dishes, requiring repeated opening and closing of the incubator lid. The transport distance is long, and there is a risk of bumps and knocks. Furthermore, prolonged opening of the lid causes fluctuations in temperature and CO2 concentration, which affects the developmental potential of the eggs.
Design a device that includes an incubator, a lid, and a foot pedal auxiliary switch. Utilize the lever principle and a servo motor to automatically control the opening and closing of the lid, reducing manual operation and improving transfer efficiency and safety.
It simplifies the transfer process of embryo culture dishes, shortens the operation time, reduces temperature and CO2 concentration fluctuations, improves transfer speed and safety, and protects the culture environment and developmental potential of eggs.
Smart Images
Figure CN223852641U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of assisted reproductive technology, and specifically relates to a device for improving embryo culture dish transfer efficiency and safety. BACKGROUND
[0002] Human assisted reproductive technology is one of the most effective techniques for treating infertility. In China, nearly 1 million infertile patients use assisted reproductive technology to have children every year, of which about 30% of infertility causes are male factors, and intracytoplasmic sperm injection (ICSI, commonly known as the second generation of in vitro fertilization technology) is mainly used to solve male factor infertility. At the same time, genetic testing technology (commonly known as the third generation of in vitro fertilization technology) also needs to use ICSI technology for fertilization. Intracytoplasmic sperm injection technology is to place sperm and eggs in an ICSI operation dish with the help of a micro-operation system and an inverted microscope, fix the egg with a fixed needle, grasp the sperm with another micro-injection needle, and inject a single sperm into the egg under a microscope to promote fertilization.
[0003] In assisted reproductive technology, all the above operations on eggs are to place the eggs in a culture dish, store the culture dish in a culture box, and then place the eggs in an ICSI operation dish to complete the operation under a microscope. The operation involves three types of culture dishes: ICSI operation dish, embryo culture dish (uninjected), and embryo culture dish (injected). The ICSI operation dish needs to be transferred back and forth between the stereoscopic microscope and the inverted microscope, and the embryo culture dish (uninjected) and the embryo culture dish (injected) need to be transferred back and forth between the stereoscopic microscope, the constant temperature plate, and the culture box to complete the transfer of the eggs between different culture dishes. Specifically, the following operation steps need to be completed for each ICSI operation:
[0004] 1. Prepare the embryo culture dish (uninjected) in advance, place the unoperated eggs in the embryo culture dish (uninjected), place the embryo culture dish (uninjected) containing the eggs in the culture box, open the box cover buckle with one hand, lift the culture box cover, take out the embryo culture dish (uninjected) with the other hand, transfer it to the stereoscopic microscope stage, immediately close the culture box cover, and buckle the buckle.
[0005] 2. Then open the buckle of the other culture box cover with one hand, lift the culture box cover, take out the ICSI operation culture dish with the other hand, transfer it to the stereoscopic microscope stage, immediately close the culture box cover, and buckle the buckle.
[0006] 3. Transfer 1-3 eggs from the embryo culture dish (uninjected) to the ICSI operation dish with a Pasteur pipette.
[0007] 4, Cover the embryo culture dish (not injected) dish cover, open the incubator cover buckle with one hand, put the embryo culture dish (not injected) with the remaining eggs on the inverted microscope stage to the incubator, close the incubator cover, buckle;
[0008] 5, the operator first go to the stereomicroscope side, with both hands to the ICSI operation dish containing 1-3 eggs from the stereomicroscope stage to the inverted microscope stage, after the completion of ICSI injection under the inverted microscope, slowly walk to the ICSI operation dish again transported to the stereomicroscope stage;
[0009] 6, use one hand to open the incubator cover buckle, the other hand to take out the dish cover marked "injected" embryo culture dish (injected), transported to the stereomicroscope stage, close the incubator cover, buckle;
[0010] 7, open the embryo culture dish (injected) dish cover, use Pasteur pipette to transfer the ICSI operation dish has completed the injection of 1-3 eggs to the embryo culture dish (injected) dish;
[0011] 8, cover the embryo culture dish (injected) dish cover, open the incubator cover buckle with one hand, put the embryo culture dish (injected) from the stereomicroscope stage to the incubator, so complete a ICSI operation.
[0012] If there are remaining ICSI injection of eggs, repeat steps 1-8, until all the eggs are injected.
[0013] In order to avoid the eggs in the incubator stay too long, according to the operation specification, only 1-3 eggs for ICSI operation, the non-injected egg culture dish is still in the incubator. If a patient with 10-20 eggs, the whole ICSI operation process, need to repeat the above 1-8 steps 7-10 times, so the cycle until all the eggs are injected. Therefore, every day countless times to open and close the incubator cover, between the stereomicroscope and the incubator transport embryo culture dish, the workload is extremely complicated.
[0014] More complicated problem is that: now the ICSI operation is usually located outside the IVF workstation, the transfer of the egg is long distance, or the incubator is embedded in the IVF workstation, but the position is far from the stereoscopic microscope, and is sunk in the IVF workstation, and the transfer of the culture dish must need two hands to cooperate, open the box cover buckle - lift the box cover - take out the culture dish - close the box cover - buckle the buckle, and then open and close the box cover and access the culture dish in sequence after the ICSI operation is completed. The path of the culture dish has horizontal direction and vertical direction, the path is far, and there is a risk of knocking the culture dish in the process of repeatedly transferring the culture dish, and once the patient's egg is spilled, it is a major medical accident, which causes great loss to the patient.
[0015] In summary, the defects of the existing ICSI operation in the transfer of the culture dish are:
[0016] As described above, the culture dish needs to be transferred 4 times for each ICSI operation, and the culture dish needs to be opened and closed 8 times, which needs to be operated by two hands, which is extremely complicated; the culture dish transfer distance is far, the culture dish transfer has up and down direction path and horizontal direction path, and there is a risk of knocking the culture dish in the process of repeatedly transferring the culture dish, and once the patient's egg is spilled, it is a major medical accident, which causes great loss to the patient; the culture dish cover is opened for a long time, which causes the temperature and CO2 concentration in the box to decrease, and the temperature and CO2 concentration cannot be recovered in time, thereby causing the fluctuation of the egg culture environment and the adverse effect on the development potential of the egg. SUMMARY
[0017] In view of the problems in the prior art, the purpose of the present application is to provide a device for improving the transfer efficiency and safety of the embryo culture dish between the incubator and the stereoscopic microscope hot plate, so as to solve the technical problems mentioned in the background art.
[0018] In order to solve the above problems, the present application adopts the following technical scheme.
[0019] A device for improving the transfer efficiency and safety of the embryo culture dish, comprising an incubator arranged on the inner surface of the IVF workstation and a box cover arranged on the incubator and capable of opening and closing the incubator, and a stereoscopic microscope arranged on the inner surface of the IVF workstation, wherein the incubator is arranged on the right side of the fixed column, and the incubator is arranged on the right front side of the heating plate of the stereoscopic microscope, and the inner surface of the IVF workstation is provided with an opening for arranging the incubator, and the incubator is embedded and fixed in the opening, and the top surface of the box body of the incubator and the inner surface of the IVF workstation are on the same horizontal plane, and the bottom of the IVF workstation is provided with a foot pedal auxiliary switch for controlling the opening and closing of the box cover.
[0020] The box cover comprises an overweight box cover body, a lever fixedly connected with the overweight box cover body, a connecting rod rotatably connected at the tail of the lever, a vertical rod rotatably connected at the tail of the connecting rod and vertically movable, a cam block for driving the vertical rod to move up and down, a mounting shell for mounting the cam block, and a servo motor for driving the cam block.
[0021] As a further scheme of the utility model, the incubator and the box cover are rotatably connected through the long hinge pin.
[0022] As a further scheme of the utility model, a sealing strip is arranged between the periphery of the incubator body and the periphery of the box cover.
[0023] As a further scheme of the utility model, the upper end surface of the box cover is at an angle of 45 degrees to the center line of the lever.
[0024] As a further scheme of the utility model, a through hole is formed in the IVF workbench surface for the movement of the lever and the connecting rod.
[0025] As a further scheme of the utility model, the servo motor is mounted on the mounting shell, the output shaft of the servo motor is axially mounted on the shaft center of the cam block, a heart-shaped track sliding groove is formed in the side of the cam block away from the servo motor, and the bottom of the vertical rod is fixed with a connecting rod with a roller, the roller on the connecting rod is rotatably connected at the end of the connecting rod, and the roller on the connecting rod is inserted into the heart-shaped track sliding groove.
[0026] As a further scheme of the utility model, the servo motor is electrically connected with the foot pedal auxiliary switch through the cable.
[0027] As a further scheme of the utility model, the foot pedal auxiliary switch comprises a control foot pedal one for controlling the forward rotation of the servo motor and a control foot pedal two for controlling the reverse rotation of the servo motor.
[0028] Compared with the prior art, the utility model has the advantages that:
[0029] 1. The incubator arranged on the workbench surface in the IVF workbench surface enables the embryo culture dish to be transferred between the stereoscopic microscope heating plate and the incubator, only needs to be translated and has the shortest stroke, increases the embryo culture dish transfer speed and safety, and makes the staff no longer overly nervous but relaxed and happy.
[0030] 2, using the principle of lever, with foot pedal auxiliary switch to open the incubator cover at a constant speed, when the hand to take away the culture dish, again to step on the pedal auxiliary switch, the cover is automatically falling to seal the box, compared to the traditional hand open cover - open button - lift the cover - wait for the culture dish to be transferred to the thermostat under the stereomicroscope - close the cover - button, with foot pedal switch control cover handle to release, foot switch cover speed is significantly faster than hand switch cover, once the culture dish is taken out of the incubator, the cover can be closed, the cover opening time is significantly shortened, the temperature and CO2 gas concentration loss is less, the temperature and CO2 gas concentration recover faster, using overweight cover, lever thrust and the four around the sealing strip can guarantee the sealing of the incubator, save the button cover link, improve the work efficiency and safety of embryo culture dish transfer, also better protect the egg culture environment and egg development potential. BRIEF DESCRIPTION OF DRAWINGS
[0031] Figure 1 It is whole structure schematic diagram of the utility model;
[0032] Figure 2 It is incubator, cover, foot pedal auxiliary switch connection structure schematic diagram;
[0033] Figure 3 It is the local structure schematic diagram of cover;
[0034] Figure 4 It is the local structure schematic diagram of cover;
[0035] Figure 5 It is the schematic diagram of cover opening and closing state.
[0036] Mark explanation in drawing:
[0037] 1, incubator; 2, cover; 3, stereomicroscope; 301, fixed column; 302, hot plate; 4, opening; 5, foot pedal auxiliary switch; 201, overweight cover body; 202, lever; 203, connecting rod; 204, vertical rod; 205, cam block; 206, installation shell; 207, servo motor; 6, sealing strip; 7, through hole; 208, heart-shaped track sliding groove; 209, cable; 210, connecting rod; 501, control foot pedal one; 502, control foot pedal two. DETAILED DESCRIPTION
[0038] The technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application; obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments; based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application. Embodiment
[0039] Please refer to Figures 1 to 5 The device for improving the embryo culture dish transfer efficiency and safety comprises a culture box 1 arranged on the IVF workstation top surface and a box cover 2 arranged on the culture box 1 and capable of opening and closing the culture box 1, a stereomicroscope 3 arranged on the IVF workstation top surface, the culture box 1 being arranged on the right side of a fixing column 301 arranged below the stereomicroscope 3 and located at the right front of a heating plate 302 of the stereomicroscope 3, an opening 4 being arranged on the IVF workstation top surface for mounting the culture box 1, the culture box 1 being embedded and fixed in the opening 4, and the top surface of the culture box 1 being on the same horizontal plane as the IVF workstation top surface, and a foot pedal auxiliary switch 5 arranged at the bottom of the IVF workstation for controlling the opening and closing of the box cover 2.
[0040] The box cover 2 comprises an overweight box cover body 201, a lever 202 fixedly connected with the overweight box cover body 201, a connecting rod 203 rotatably connected at the tail of the lever 202, a vertical rod 204 rotatably connected at the tail of the connecting rod 203 and capable of moving vertically, a cam block 205 for driving the vertical rod 204 to move up and down, a mounting shell 206 for mounting the cam block 205, and a servo motor 207 for driving the cam block 205.
[0041] In the embodiment, the culture box 1 and the box cover 2 are rotatably connected through a long hinge pin 8.
[0042] In the embodiment, a sealing strip 6 is arranged between the culture box 1 and the box cover 2.
[0043] In the embodiment, the upper end surface of the box cover 2 is at an angle of 45° with the center line of the lever 202.
[0044] In the embodiment, a through hole 7 is arranged on the IVF workstation top surface for the movement of the lever 202 and the connecting rod 203.
[0045] In the embodiment, the servo motor 207 is mounted on the mounting shell 206, and the output shaft of the servo motor 207 is mounted on the shaft center of the cam block 205, a heart-shaped track sliding groove 208 is arranged on the side of the cam block 205 away from the servo motor 207, a connecting rod 210 with a roller is fixed at the bottom of the vertical rod 204, the roller on the connecting rod 210 is rotatably connected at the end of the connecting rod 210, and the roller on the connecting rod 210 is inserted into the heart-shaped track sliding groove 208.
[0046] In the embodiment, the servo motor 207 is electrically connected with the foot pedal auxiliary switch 5 through a cable 209.
[0047] In the embodiment, the foot pedal auxiliary switch 5 comprises a control foot pedal one 501 for controlling the forward rotation of the servo motor 207 and a control foot pedal two 502 for controlling the reverse rotation of the servo motor 207.
[0048] The working process of the utility model is as follows:
[0049] The operator can
[0050] The three kinds of prepared embryo culture dishes are placed in the incubator 1, at the beginning of the operation, the incubator 1 box cover 2 is automatically opened at a uniform speed by stepping on the foot pedal auxiliary switch 5, after the culture dish (not injected) is taken out by hand, the box cover 2 is automatically and uniformly lowered to seal the box body by stepping on the foot pedal auxiliary switch 5 again, at this time, the embryo culture dish (not injected) has been transferred to the stage of the stereomicroscope 3, the ICSI operation culture dish is taken out from the incubator 1 and transferred to the stage of the stereomicroscope 3 in the same way; 1-3 ova are transferred from the embryo culture dish (not injected) to the ICSI operation dish by using a Pasteur pipette; the box cover 2 of the incubator 1 is opened by using the foot pedal, the embryo culture dish (not injected) with the remaining ova is transferred from the stage of the stereomicroscope 3 to the incubator 1; after the ICSI injection is completed under the inverted microscope, the ICSI operation dish is transferred to the stage of the stereomicroscope 3 by hand slowly; the box cover 2 of the incubator 1 is opened by using the foot pedal, the embryo culture dish (injected) with the label "injected" is taken out and transferred to the stage of the stereomicroscope 3, the ova in the ICSI dish that have been injected are transferred to the embryo culture dish (injected) by using a Pasteur pipette, the embryo culture dish (injected) is transferred to the incubator 1 by using the foot pedal to open the box cover 2 of the incubator 1, and the box cover 2 of the incubator 1 is closed, so that one ICSI operation is completed.
[0051] If there are remaining ova that have not been injected, the above steps are repeated until all the ova are injected.
[0052] 1. By arranging the incubator 1 on the table top in the IVF workstation table top, the embryo culture dish is transferred between the stage of the stereomicroscope 3 and the incubator 1, only the culture dish needs to be translated and the stroke is the shortest, the speed and safety of the embryo culture dish transfer are increased, the staff is no longer excessively nervous, but relaxed and happy.
[0053] 2, using the principle of lever, with foot pedal auxiliary switch 5 automatic uniform speed open incubator 1 box cover 2, when using hand to take away the culture dish, again kick pedal auxiliary switch 5, box cover 2 automatic uniform speed down seal box, at the same time, using the principle of lever and sealing strip further increases the force of the box cover seal incubator, compared with the traditional hand open box cover 2 - open button - lift up the box cover 2 - wait for the culture dish to be transferred to the stereoscopic microscope 3 under the constant temperature plate - close box cover 3 - button, with foot pedal switch 5 control box cover 2 handle liberation, foot switch box cover 2 speed is obviously faster than hand switch box cover 2, the box cover 2 opening time is obviously shortened, the temperature and CO2 gas concentration loss is less, the temperature and CO2 gas concentration recovers predetermined value faster, improves the work efficiency and safety of embryo culture dish transfer, also better protects the egg culture environment and egg development potential.
[0054] The above merely provides the preferred embodiments of the present application; however, the protection scope of the present application is not limited to this. Any person skilled in the art, within the technical range disclosed by the present application, according to the technical scheme and improvement concept of the present application, should be covered in the protection scope of the present application.
Claims
1. A device for improving embryo culture dish transfer efficiency and safety, comprising a culture box (1) arranged on the countertop in an IVF workstation and a box cover (2) arranged on the culture box (1) and capable of opening and closing the culture box (1), wherein a stereomicroscope (3) is arranged on the countertop in the IVF workstation, and the stereomicroscope (3) is installed on the countertop in the IVF workstation through a fixed column (301) arranged below the stereomicroscope (3), characterized in that: The incubator (1) is arranged at the right side of the fixed column (301), and the incubator (1) is located at the right front of the heating plate (302) below the objective lens of the stereomicroscope (3), the opening (4) for installing the incubator (1) is arranged on the inner tabletop of the IVF workstation, the incubator (1) is fixedly arranged in the opening (4), and the top surface of the incubator (1) is on the same horizontal plane as the inner tabletop of the IVF workstation, and the auxiliary foot switch (5) for controlling the opening and closing of the box cover (2) is arranged at the bottom of the IVF workstation. The box cover (2) comprises an overweight box cover body (201), a lever (202) fixedly connected with the overweight box cover body (201), a connecting rod (203) rotatably connected at the tail of the lever (202), a vertical rod (204) vertically movably connected at the tail of the connecting rod (203), a cam block (205) for driving the vertical rod (204) to move up and down, a mounting shell (206) for mounting the cam block (205), and a servo motor (207) for driving the cam block (205).
2. The device for improving the efficiency and safety of embryo dish transfer according to claim 1, characterized in that: The incubator (1) and the box cover (2) are rotatably connected through the long hinge pin (8).
3. The device for improving the efficiency and safety of embryo dish transfer according to claim 2, characterized in that: Sealing strips (6) are arranged between the top surface of the incubator (1) and the bottom surface of the box cover (2).
4. The device for improving the efficiency and safety of embryo dish transfer according to claim 1, characterized in that: The upper end surface of the box cover (2) is at an angle of 45° with the center line of the lever (202).
5. The device for improving the efficiency and safety of embryo dish transfer according to claim 4, characterized in that: A through hole (7) for the movement of the lever (202) and the connecting rod (203) is arranged on the IVF workstation tabletop.
6. The device for improving the efficiency and safety of embryo dish transfer according to claim 5, characterized in that: The servo motor (207) is mounted on the mounting shell (206), and the output shaft of the servo motor (207) is axially mounted on the shaft center of the cam block (205), the cam block (205) is provided with a heart-shaped track sliding groove (208) at the side away from the servo motor (207), and the bottom of the vertical rod (204) is fixedly provided with a connecting rod (210) provided with a roller, that is, the roller on the connecting rod (210) is rotatably connected at the end of the connecting rod (210), and the roller on the connecting rod (210) is inserted into the heart-shaped track sliding groove (208).
7. The device for improving the efficiency and safety of embryo dish transfer according to claim 1, characterized in that: The servo motor (207) is electrically connected with the auxiliary foot switch (5) through the cable (209).
8. The device for improving the efficiency and safety of embryo dish transfer according to claim 7, characterized in that: The auxiliary foot switch (5) comprises a control foot switch one (501) for controlling the forward rotation of the servo motor (207) and a control foot switch two (502) for controlling the reverse rotation of the servo motor (207).