Device for conveniently collecting and measuring bemisia tabaci honeydew

Abstract

The utility model provides a device for conveniently collecting and measuring bemisia tabaci honeydew. The device comprises a transparent cylindrical tube, a top cover is arranged at the top of the cylindrical tube, a base is arranged at the bottom of the cylindrical tube, and the cylindrical tube is sealed up and down; an air circulation window is formed in one side of the side wall of the cylindrical pipe; and the other side is provided with a small hole. An interlayer is arranged on the lower surface of the top cover, and agar and cotton leaves are placed in the interlayer. A groove is formed in the upper surface of the base, and bromocresol green filter paper is placed in the groove. The device is simple and convenient, cotton leaves are used for feeding, a relatively independent environment can be formed, sufficient air and food are provided for growth of test insects, the test insects can be prevented from escaping, interference caused by factors such as overlapping of plant leaves is avoided, and convenience and accuracy of bemisia tabaci honeydew collection and determination are improved. According to the bemisia tabaci honey dew collecting device, honey dew is collected through the modified transparent tube, experimental errors caused by flying activities of bemisia tabaci are reduced by utilizing a limited space, the space is saved, the cost is low, and the operation is convenient.

Description

Technical Field

[0001] This utility model relates to a device for convenient collection and measurement of honeydew from whiteflies, belonging to the field of experimental equipment technology. Background Technology

[0002] The whitefly is a global agricultural pest that damages crops by sucking sap from the phloem of plants, causing chlorosis, spreading plant viruses, and secreting honeydew, which causes sooty mold. Honeydew secretion is an important indicator in the study of the biology and harmful characteristics of whiteflies. Whiteflies are small and secrete very little honeydew, making convenient collection and accurate measurement of honeydew particularly necessary. However, most existing whitefly honeydew collection devices collect honeydew dripping from the leaves of host plants by placing an aluminum foil under the leaves. But the shortcomings of this method are obvious: (1) Some of the honeydew secreted by whiteflies will remain on the leaves of the host plants, especially when the plant branches and leaves are dense or the location of the honeydew secretion is special. The honeydew may not be able to drip smoothly onto the aluminum foil, resulting in low collection efficiency and poor accuracy. (2) The physical properties of the aluminum foil itself may affect whiteflies. The aluminum foil may cause changes in the physiological activities of whiteflies due to its own reflection. (3) Whiteflies have a wide range of activity and flight range. It is difficult to control the number of insects per unit volume when collecting them by placing aluminum foil directly under their host plants, which will cause deviations in the accurate measurement of honeydew. (4) Whiteflies have a wide range of hosts. If the host is a plant with large leaves such as cucumber or eggplant, the collection area under the leaves of the aluminum foil is large. During the washing and collection, the honeydew concentration will be too low due to the large volume of deionized water used, which will increase the difficulty of measurement and increase the error.

[0003] Therefore, a convenient device for collecting and measuring honeydew from whiteflies is proposed to address the shortcomings of existing technologies. Summary of the Invention

[0004] To address the aforementioned technical problems, this utility model provides a convenient device for collecting and measuring honeydew from whiteflies, comprising: a transparent cylindrical tube, the top of which is provided with a top cover and the bottom with a base to seal the top and bottom of the cylindrical tube; an air circulation window is provided on one side of the cylindrical tube; and a small hole is provided on the other side.

[0005] The cylindrical tube is provided with a breathable mesh at the air circulation window.

[0006] The lower surface of the top cover is provided with a sandwich layer, and agar and cotton leaves are placed inside the sandwich layer.

[0007] The base has a groove on its upper surface, and bromocresol green filter paper is placed in the groove as a collection paper for collecting honeydew.

[0008] The small hole is equipped with a sealing film. The small hole is used to put the whitefly into a 0.2mL PCR tube with the bottom cut off. After the whitefly is put in, the small hole is sealed with the sealing film.

[0009] Preferably, the cylindrical tube has an inner diameter of 26mm, an outer diameter of 30mm, and a height of 40mm; the air circulation window is approximately 20mm long and 20mm wide; and the small hole has a diameter of 2-3mm.

[0010] This invention employs a cylindrical tube with a breathable mesh on one side to ensure proper air circulation within the device and facilitate timely observation of the test insects. Agar is placed inside the top cover to keep the cotton leaves fresh, allowing the test insects to feed and survive normally. The collection paper is positioned directly beneath the cotton leaves, allowing for direct collection of honeydew secreted by the whiteflies, and the paper can be replaced at any time. This device confines the whiteflies to a specific spatial area for feeding and honeydew secretion, reducing experimental errors caused by the whiteflies' excessive activity range. Furthermore, the device is simple in design, low in cost, reusable, and easy to operate. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the structure of the whitefly honeydew collection and measurement device of this utility model. Detailed Implementation

[0012] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0013] like Figure 1 As shown, the device for collecting and measuring honeydew from whiteflies using cotton leaves includes a transparent cylindrical tube with an inner diameter of 26 mm, an outer diameter of 30 mm, and a height of 40 mm; a breathable mesh 2 about 20 mm long and 20 mm wide is provided on the side of the cylindrical tube; a top cover 3 is provided at the top, and a base 4 is provided at the bottom; a small hole 5 with a diameter of 2-3 mm is provided on the side of the cylindrical tube.

[0014] The interlayer of the top cover 3 contains 0.1% agar, and cotton leaves with the same inner diameter as the top cover 3 are placed on the agar, with the back of the leaves facing outward.

[0015] A bromocresol green filter paper 6 with the same inner diameter as the base is placed in the groove of the base 4 for collecting and measuring honeydew.

[0016] The cylindrical tube has a small hole 5 on its side for inserting whiteflies, which are then sealed with sealing film.

[0017] Honeydew collection and measurement methods:

[0018] Prepare 0.1% agar and pour it into the interlayer of the top cover 3. Use a punch to make cotton leaves with the same size as the inner diameter of the top cover 3. After the agar solidifies, place the leaves with the back facing outwards on the agar to keep them moist.

[0019] To prepare a 0.1% bromocresol green solution, accurately weigh 0.1 g of bromocresol green, dissolve it thoroughly in 95% ethanol solution, and bring the volume to 100 mL.

[0020] Immerse the filter paper in a 0.1% bromocresol green solution, then remove it and air dry it naturally or dry it in an oven at 75°C. At this point, the filter paper will be a bright orange-yellow color. After drying, prepare a bromocresol green filter paper 6 with the same inner diameter as the base, and place the bromocresol green filter paper 6 in the base 4.

[0021] The male and female adult whiteflies were placed into the cylindrical tube through the small hole 5 on the side wall; after placement, the small hole 6 was sealed with sealing film.

[0022] The insect tubes were placed in an artificial climate chamber, and the culture conditions were 27°C, 60% relative humidity, and a photoperiod of 16 hours light: 8 hours darkness.

[0023] The 0.1% bromocresol green filter paper was replaced every 48 hours, and the area of ​​the stain was calculated using ImageJ software. Two samples were collected in total, and the results are as follows:

[0024] After 48 hours, the bromocresol green collection paper was removed and replaced with new paper. The area of ​​the bromocresol green spots on the collected honeydew was calculated using ImageJ software, yielding the honeydew secretion data of male and female whiteflies from 0 to 48 hours (Table). Analysis of the honeydew collection and measurement data of male and female whiteflies shows that the average honeydew secretion area per female was significantly higher than that per male. P <0.001).

[0025] Table 1. Honeydew secretion of male and female whiteflies from 0 to 48 hours.

[0026]

[0027] Table 2. Honeydew secretion of male and female whiteflies 48-96 h

[0028]

[0029] After 96 hours, the bromocresol green collection paper was removed, and the area of ​​the colored spots on the bromocresol green collection paper after honeydew collection was calculated using ImageJ software. The honeydew secretion of male and female whiteflies from 48 to 96 hours can be obtained (Table 2). The analysis of the honeydew collection and measurement of male and female whiteflies shows that the average honeydew secretion area per female is significantly higher than that per male. P <0.001).

Claims

1. A convenient device for collecting and measuring honeydew from whiteflies, characterized in that, include: A transparent cylindrical tube (1) is provided with a top cover (3) at the top and a base (4) at the bottom to seal the cylindrical tube (1) from top to bottom; an air circulation window is provided on one side of the cylindrical tube (1); and a small hole (5) is provided on the other side.

2. The device for convenient collection and measurement of honeydew from whiteflies according to claim 1, characterized in that, The cylindrical tube (1) is provided with a breathable mesh (2) at the air circulation window.

3. The device for convenient collection and measurement of honeydew from whiteflies according to claim 1, characterized in that, The lower surface of the top cover (3) is provided with a sandwich layer, and agar and cotton leaves are placed in the sandwich layer.

4. The device for convenient collection and measurement of honeydew from whiteflies according to claim 1, characterized in that, The upper surface of the base (4) is provided with a groove, and bromocresol green filter paper (6) is placed in the groove.

5. The device for convenient collection and measurement of honeydew from whiteflies according to claim 1, characterized in that, A sealing film is provided at the small hole (5). After the whitefly is put in, the small hole (5) is sealed with the sealing film.

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