Nucleic acid extraction suction head

Abstract

The utility model relates to the technical field of nucleic acid, and particularly discloses a nucleic acid extraction suction head which comprises a suction head body, and the suction head body comprises a head part, a neck part and a tube part which are sequentially connected from top to bottom; a water-sealed filter element is arranged in the head part, and when liquid enters the head part, the water-sealed filter element expands when meeting water and seals an internal channel of the water-sealed filter element; a spiral flow guide groove is formed in the inner wall of the neck part and extends from the upper end of the neck part to the lower end of the neck part. According to the utility model, the nucleic acid extraction process can be simplified, the extraction efficiency can be improved, the sample loss can be reduced, and cross contamination can be effectively prevented through the water-sealed filter element on the uppermost layer of the suction head.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid extraction technology, specifically to a nucleic acid extraction pipette tip. Background Technology

[0002] Nucleic acid extraction is a crucial fundamental procedure in many fields, including molecular biology research, medical diagnostics, and gene sequencing. Traditional nucleic acid extraction methods often rely on complex instruments and cumbersome procedures, such as centrifuges and magnetic racks. These methods have several drawbacks in sample processing, as detailed below:

[0003] Long operation time: Due to the use of multiple instruments and equipment and the complex operation process, the entire nucleic acid extraction process takes a lot of time, which affects work efficiency.

[0004] Significant sample loss: During sample transfer, centrifugation, and other operations, improper operation or equipment limitations can easily cause samples to adhere to the instrument surface or fail to be completely collected, resulting in sample loss and affecting the accuracy of subsequent testing.

[0005] Low extraction efficiency: The complex operation process and the coordinated operation of multiple devices increase the possibility of errors, and the connection between each step may not be smooth enough, which leads to low nucleic acid extraction efficiency.

[0006] Cross-contamination is likely to occur: During the processing of different samples, it is difficult to completely clean and disinfect the instruments and equipment used, especially in high-throughput detection. Cross-contamination between samples is likely to occur, which seriously affects the reliability of the test results.

[0007] Therefore, those skilled in the art have provided a nucleic acid extraction pipette tip. Utility Model Content

[0008] The purpose of this invention is to provide a nucleic acid extraction pipette tip that simplifies the nucleic acid extraction process, improves extraction efficiency, reduces sample loss, and effectively prevents cross-contamination through the water-sealed filter element at the top of the pipette tip.

[0009] The objective of this utility model can be achieved through the following technical solutions:

[0010] A nucleic acid extraction pipette tip includes a pipette tip body, wherein the pipette tip body comprises a head portion, a neck portion, and a tube portion connected sequentially from top to bottom;

[0011] The head section is equipped with a water-sealed filter element. When liquid enters the head section, the water-sealed filter element expands upon contact with water and seals its internal channels.

[0012] The inner wall of the neck portion is provided with a spiral guide groove, which extends from the upper end to the lower end of the neck portion.

[0013] Furthermore, the lower end of the tube portion is provided with a tube bevel, which is in the shape of a beveled cut.

[0014] Furthermore, a sample filter element is provided inside the tube portion;

[0015] Furthermore, the outer surface of the head portion is fixedly connected with multiple outwardly protruding strips at intervals;

[0016] Furthermore, the head portion, neck portion, and tube portion are integrally formed.

[0017] The beneficial effects of this utility model are:

[0018] 1. The nucleic acid extraction pipette tip proposed in this utility model effectively prevents cross-contamination through a water-sealed filter element inside the head part; during the nucleic acid extraction process, if the pipette tip accidentally comes into contact with other contaminants after aspirating a sample containing nucleic acid, the water-sealed filter element can prevent the contaminants from entering the pipette tip, ensuring the purity of the sample in the next aspiration operation.

[0019] 2. The present invention proposes a nucleic acid extraction pipette tip, wherein the spiral guide groove on the inner wall of the neck portion of the pipette tip improves the nucleic acid extraction efficiency; the liquid forms a spiral flow under the guidance of the spiral guide groove, which increases the contact opportunity and time between the nucleic acid and the adsorption material on the inner wall of the pipette tip, so that more nucleic acid can be effectively adsorbed. Compared with traditional pipette tips, it can significantly improve the efficiency of nucleic acid extraction.

[0020] 3. The nucleic acid extraction pipette tip proposed in this utility model has a beveled design at the lower end of the pipette tube to reduce sample loss. The beveled design can better reach the bottom corners of the sample container and more thoroughly extract the sample, avoiding the sample residue problem that may be left by traditional flat-cut pipette tips, ensuring the integrity of sample extraction, thereby reducing sample loss and improving the accuracy of subsequent test results.

[0021] 4. The present invention proposes a nucleic acid extraction pipette tip, wherein the pipette tube is provided with a sample filter element, which can filter sample impurities and ensure extraction efficiency. Attached Figure Description

[0022] The present invention will be further described below with reference to the accompanying drawings.

[0023] Figure 1 This is an overall structural diagram of a nucleic acid extraction pipette tip proposed in this utility model;

[0024] Figure 2This is a cross-sectional view of a nucleic acid extraction pipette tip proposed in this utility model;

[0025] Figure 3 This is a top view of the spiral guide groove in a nucleic acid extraction pipette tip proposed in this utility model.

[0026] Figure label:

[0027] 1. Head section; 2. Neck section; 3. Tube section; 4. Outward protrusion; 5. Slanted opening of the tube; 6. Spiral guide groove; 7. Water-sealed filter element; 8. Sample filter element. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0029] Please see the appendix Figures 1 to 3 As shown in the figure, a nucleic acid extraction pipette tip in this embodiment of the present invention includes a pipette tip body, which includes a head portion 1, a neck portion 2 and a tube portion 3 connected sequentially from top to bottom. The head portion 1, the neck portion 2 and the tube portion 3 are integrally formed and are conical, which is strong and easy to use.

[0030] The head part 1 is equipped with a water-sealed filter element 7. When liquid enters the head part 1, the water-sealed filter element 7 expands upon contact with water and seals its internal channels. The water-sealed filter element 7 has the characteristic of sealing upon contact with water. When liquid enters the head part 1, the water-sealed filter element 7 expands upon contact with water and seals its internal channels, preventing liquid backflow and external contaminants from entering the suction head.

[0031] The inner wall of the neck portion 2 is provided with a spiral guide groove 6, which extends from the upper end to the lower end of the neck portion 2. Its function is to guide the liquid to form a spiral flow along the guide groove during the liquid aspiration and discharge process, increase the contact area between the liquid and the inner wall of the pipette tip, thereby improving the binding efficiency of nucleic acid with the adsorption material on the inner wall of the pipette tip (which can be achieved by pre-setting a specific nucleic acid adsorption coating on the inner wall of the pipette tip, etc.), and thus improving the nucleic acid extraction efficiency.

[0032] The nucleic acid adsorption coating can be made of polymer materials, silicon-based materials, etc.

[0033] As one embodiment of this utility model, the lower end of the tube portion 3 is provided with a tube bevel 5. The tube bevel 5 is in the shape of a bevel cut. Compared with the traditional flat cut, the tube bevel 5 in the shape of a bevel cut can be inserted into the corner of the bottom of the sample container more conveniently when aspirating the sample, reducing sample residue, improving the integrity of sample aspiration, and thus reducing sample loss.

[0034] As one embodiment of this utility model, the tube portion 3 is provided with a sample filter element 8 inside, which can filter sample impurities and ensure extraction efficiency.

[0035] As one embodiment of this utility model, a plurality of protruding strips 4 are fixedly connected at intervals on the outer surface of the head part 1. The protruding strips 4 can improve the roughness of the outer surface of the head part 1, thereby enhancing the friction and making it easier to disassemble and replace the suction head.

[0036] Working principle: The nucleic acid extraction tip of this invention is installed on a pipette. When a sample containing nucleic acid needs to be aspirated, the beveled end of the tube section 3 is inserted into the sample container. Due to the beveled design, it can be easily inserted into various corners of the bottom of the sample container, ensuring maximum sample aspiration. During aspiration, the sample solution enters the neck section 2 through the tube section 3. At this time, the spiral guide groove 6 on the inner wall of the neck section 2 guides the sample solution to form a spiral flow, increasing the contact area between the sample solution and the adsorbent material on the inner wall of the tip (if a nucleic acid adsorption coating has been pre-set), causing some nucleic acids to begin to bind to the adsorbent material.

[0037] In addition, to prevent cross-contamination:

[0038] When the tip 1 comes into contact with a sample containing nucleic acid, the water-reactive filter 7 inside the tip 1 expands upon contact with water and seals its internal channels. During subsequent operations, even if the pipette tip accidentally comes into contact with other contaminants, such as other samples or the surface of the workbench, the water-reactive filter 7 prevents these contaminants from entering the pipette tip, thus effectively preventing cross-contamination.

[0039] Post-nucleic acid extraction procedures:

[0040] After sample aspiration, subsequent operations can be performed according to the specific nucleic acid extraction method, such as adding lysis buffer and washing solution. Throughout the process, the spiral guide groove 6 in the neck section 2 has already facilitated the binding of some nucleic acids with the adsorbent material, which can further improve the integrity and efficiency of nucleic acid extraction in subsequent processing.

[0041] In summary, the nucleic acid extraction pipette tip of this invention plays an important role in all aspects of nucleic acid extraction, effectively solving many problems existing in traditional nucleic acid extraction, and achieving the goals of simplifying the process, improving efficiency, reducing sample loss, and preventing cross-contamination.

[0042] The above description provides a detailed account of one embodiment of the present invention. However, this description is merely a preferred embodiment and should not be construed as limiting the scope of the present invention. All equivalent variations and improvements made within the scope of the claims of the present invention should still fall within the patent coverage of the present invention.

Claims

1. A nucleic acid extraction pipette tip, comprising a pipette tip body, characterized in that: The suction head body includes a head part (1), a neck part (2) and a tube part (3) connected from top to bottom; The head part (1) is provided with a water-sealed filter element (7). When liquid enters the head part (1), the water-sealed filter element (7) expands upon contact with water and seals its internal channels. The inner wall of the neck portion (2) is provided with a spiral guide groove (6), which extends from the upper end to the lower end of the neck portion (2).

2. The nucleic acid extraction pipette tip according to claim 1, characterized in that: The lower end of the tube section (3) is provided with a tube bevel (5), which is a beveled cut.

3. The nucleic acid extraction pipette tip according to claim 1, characterized in that: The tube section (3) is equipped with a sample filter element (8).

4. The nucleic acid extraction pipette tip according to claim 1, characterized in that: The outer surface of the head portion (1) is fixedly connected with multiple protruding strips (4) at intervals.

5. A nucleic acid extraction pipette tip according to claim 1, characterized in that: The head portion (1), neck portion (2), and tube portion (3) are integrally formed.

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