Automatic stirring nanometer antibody culture fermentation tank

Abstract

The utility model relates to an automatic stirring nanometer antibody culture fermentation tank which comprises a nanometer antibody fermentation tank body, a stirring mechanism is arranged in the middle of the nanometer antibody fermentation tank body, and a group of limiting components are arranged outside the stirring mechanism in parallel; the stirring mechanism comprises a motor, and the motor is arranged at the top of the nano antibody fermentation tank body. The electric push rod effectively drives the movable frame and the stirring rod to move downwards until the stirring rod reaches the interior of a culture dish, and then the motor effectively drives the I-shaped shaft and the rotating shaft to rotate forwards and backwards in a reciprocating manner; after stirring is finished, a movable frame and the stirring rod are effectively driven to move upwards through an electric push rod, so that an auxiliary groove frame scrapes the residual microorganisms such as the bacteria or the yeasts on the surface of the stirring rod, the stirring rod is driven to rotate forwards and backwards in a reciprocating manner in the culture dish, and the fermentation efficiency is improved. The surface of the stirring rod can be cleaned while the waste of microorganisms such as bacteria or yeast is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to nanometer antibody culture technical field especially, it relates to a kind of automatic stirring nanometer antibody culture fermentation tank. BACKGROUND

[0002] Nanometer antibody is also called sdAb, is derived from single domain heavy chain antibody of Camelidae. Nanometer antibody its molecular weight is small, high stability, specificity is strong, easy to produce, weak immunogenicity, is widely used in biological medicine research and development, clinical in vitro diagnosis and nuclear medicine field. Nanometer antibody can be produced in large quantities in microorganism such as bacteria or yeast by genetic engineering technology, and the production cost is low. Fermentation tank needs to be used when nanometer antibody is cultured.

[0003] The utility model provides a kind of nanometer antibody filler preparation is used incubator, including incubator body, the front of the incubator body is hinged with sealing door by hinge, the left side of the lower end of the front of the incubator body is fixedly connected with PLC controller, the right side of the lower end of the front of the incubator body is fixedly connected with liquid crystal display, the left side of the incubator body inner chamber bottom is fixedly connected with semiconductor refrigerator, the right side of the incubator body inner chamber bottom is fixedly connected with electric heater;The utility model is by being provided with PLC controller, liquid crystal display, semiconductor refrigerator, electric heater, first temperature sensor, second temperature sensor and humidity sensor, has the effect that the incubation temperature of the incubator body inner chamber can be monitored, so that the incubation temperature of the incubator body inner chamber can be conveniently adjusted, and the incubation temperature of biological cell can be effectively guaranteed, and the incubation efficiency of biological cell is effectively improved.

[0004] However, the above-mentioned nanometer antibody filler preparation incubator still has the following disadvantages:

[0005] The above device is difficult to stir the nanometer antibody during the fermentation process, so the fermentation process is easily affected. If the incubator body is opened during the nanometer antibody fermentation process to stir the microorganisms such as bacteria or yeast, the fermentation temperature is easily changed, which affects the synthesis and secretion of nanometer antibody. Therefore, we propose an automatic stirring nanometer antibody culture fermentation tank. UTILITY MODEL CONTENTS

[0006] The utility model aims at overcoming the deficiencies of the prior art, adapting to the actual needs, providing an automatic stirring nanometer antibody culture fermentation tank to solve the technical problem that the current device is difficult to stir the nanometer antibody during the fermentation process, so the fermentation process is easily affected. If the incubator body is opened during the nanometer antibody fermentation process to stir the microorganisms such as bacteria or yeast, the fermentation temperature is easily changed, which affects the synthesis and secretion of nanometer antibody.

[0007] In order to achieve the purpose of the utility model, the utility model adopts the technical scheme that designs an automatic stirring nanobody culture fermentation tank, which comprises a nanobody fermentation tank body, a stirring mechanism is arranged in the middle of the nanobody fermentation tank body, a group of limiting assemblies are arranged in parallel outside the stirring mechanism,

[0008] The stirring mechanism comprises a motor, the motor is arranged on the top of the nanobody fermentation tank body, a work-shaped shaft is connected to the movable end of the motor, a rotating shaft is fixedly connected to the bottom of the work-shaped shaft, a group of auxiliary groove frames are fixedly sleeved outside the rotating shaft, a group of movable frames are symmetrically clamped inside the auxiliary groove frames, an electric push rod is connected to the horizontal surface of the movable frame, and a stirring rod is fixedly connected to the bottom end of the vertical part of the movable frame.

[0009] Preferably, the group of auxiliary groove frames form a rotating structure with the rotating shaft, the work-shaped shaft and the motor, and the vertical part of the movable frame is clamped in the auxiliary groove frame.

[0010] Preferably, the movable frame and the auxiliary groove frame form a lifting structure through the electric push rod, and the stirring rod at the bottom end of the movable frame can be matched and clamped in the auxiliary groove frame.

[0011] Preferably, the limiting assembly comprises a limiting frame, a group of limiting frames are sleeved in parallel outside the rotating shaft, a group of connecting blocks are symmetrically fixedly arranged on the outer surface of the limiting frame, a group of clamping grooves are symmetrically arranged on the inner surface of the limiting frame, and a group of culture dishes are symmetrically clamped inside the limiting frame.

[0012] Preferably, the limiting frame is fixedly connected with the nanobody fermentation tank body through the connecting block, and the rotating shaft can rotate freely inside the limiting frame.

[0013] Preferably, the culture dish and the clamping block are integrally formed, and the culture dish and the clamping groove form a clamping structure through the clamping block.

[0014] Compared with the prior art, the utility model has the beneficial effects that:

[0015] 1. The electric push rod effectively drives the movable frame and the stirring rod to move downward until the stirring rod reaches the inside of the culture dish, then the motor effectively drives the work-shaped shaft and the rotating shaft to rotate forward and backward reciprocatingly, thereby driving the stirring rod to rotate forward and backward reciprocatingly in the culture dish, realizing the stirring of bacteria or yeast and other microorganisms in the culture dish, improving the fermentation efficiency, and after the stirring is completed, the electric push rod effectively drives the movable frame and the stirring rod to move upward, so that the auxiliary groove frame scrapes the bacteria or yeast and other microorganisms remaining on the surface of the stirring rod, reduces the waste of the bacteria or yeast and other microorganisms, and can clean the surface of the stirring rod.

[0016] 2. The utility model discloses when the stirring rod is in the auxiliary groove frame inside, and the operation space is left between auxiliary groove frame and spacing frame for the culture dish to go down and move and put or take out, and through the cooperation between the clamping block and the clamping groove, the culture dish can be effectively placed stably in the spacing frame, and the culture dish is prevented from rotating together with the stirring rod when the stirring rod rotates. BRIEF DESCRIPTION OF DRAWINGS

[0017] Fig. 1 It is the whole structure schematic diagram of the utility model;

[0018] Fig. 2 It is the local section structure schematic diagram of the nanometer antibody fermentation tank body of the utility model;

[0019] Fig. 3 It is the local partial dismounting structure schematic diagram of the utility model;

[0020] Fig. 4 It is the local partial dismounting structure schematic diagram of the utility model from another angle;

[0021] In the drawing: 1, nanometer antibody fermentation tank body; 2, stirring mechanism; 3, spacing component;

[0022] 201, motor; 202, I-shaped shaft; 203, rotating shaft; 204, auxiliary groove frame; 205, movable frame; 206, electric push rod; 207, stirring rod;

[0023] 301, spacing frame; 302, connecting block; 303, clamping groove; 304, culture dish; 305, clamping block. DETAILED DESCRIPTION

[0024] The utility model is further explained in connection with the drawings and examples:

[0025] An automatic stirring nanometer antibody culture fermentation tank, see Figs. 1 to 4 Including nanometer antibody fermentation tank body 1, the middle part of nanometer antibody fermentation tank body 1 is equipped with stirring mechanism 2, and a group of spacing components 3 are arranged in parallel outside stirring mechanism 2;

[0026] The stirring mechanism 2 comprises a motor 201 arranged at the top of the nanobody fermentation tank body 1, a work-shaped shaft 202 connected to the movable end of the motor 201, a rotating shaft 203 fixedly connected to the bottom of the work-shaped shaft 202, a plurality of auxiliary groove frames 204 fixedly sleeved outside the rotating shaft 203, a plurality of movable frames 205 symmetrically clamped inside the auxiliary groove frames 204, wherein the plurality of auxiliary groove frames 204 form a rotating structure with the motor 201 through the rotating shaft 203 and the work-shaped shaft 202, the movable frame 205 is clamped through the vertical part of the movable frame 205 inside the auxiliary groove frame 204, the surface of the horizontal part of the movable frame 205 is connected with an electric push rod 206, the vertical part of the movable frame 205 is fixedly connected with a stirring rod 207 at the bottom end, further, the movable frame 205 forms a lifting structure with the auxiliary groove frame 204 through the electric push rod 206, and the stirring rod 207 at the bottom end of the movable frame 205 is matched and clamped inside the auxiliary groove frame 204. The electric push rod 206 effectively drives the movable frame 205 and the stirring rod 207 to move downward until the stirring rod 207 reaches the inside of the culture dish 304, then the work-shaped shaft 202 and the rotating shaft 203 are effectively driven by the motor 201 to rotate forward and backward reciprocatingly, so as to drive the stirring rod 207 to rotate forward and backward reciprocatingly inside the culture dish 304, realize the stirring of the bacteria or yeast and other microorganisms inside the culture dish 304, improve the fermentation efficiency, after the stirring is completed, the movable frame 205 and the stirring rod 207 are effectively driven by the electric push rod 206 to move upward, so that the bacteria or yeast and other microorganisms remaining on the surface of the stirring rod 207 are scraped by the auxiliary groove frame 204, which reduces the waste of the bacteria or yeast and other microorganisms and can clean the surface of the stirring rod 207.

[0027] It is worth noting that the limiting component 3 comprises a limiting frame 301, a plurality of limiting frames 301 are parallelly sleeved outside the rotating shaft 203, a plurality of connecting blocks 302 are symmetrically and fixedly arranged on the outer surface of the limiting frame 301, wherein the limiting frame 301 is fixedly connected with the nanobody fermentation tank body 1 through the connecting block 302, the rotating shaft 203 can freely rotate inside the limiting frame 301, a plurality of clamping grooves 303 are symmetrically arranged on the inner surface of the limiting frame 301, a plurality of culture dishes 304 are symmetrically clamped inside the limiting frame 301, a clamping block 305 is fixedly arranged on the surface of the culture dish 304, further, the culture dish 304 and the clamping block 305 are integrally formed, and the culture dish 304 forms a clamping structure with the clamping groove 303 through the clamping block 305. When the stirring rod 207 is inside the auxiliary groove frame 204, an operation space is left between the auxiliary groove frame 204 and the limiting frame 301 for the culture dish 304 to move downward and put in or move upward and take out, the culture dish 304 can be stably placed inside the limiting frame 301 through the cooperation between the clamping block 305 and the clamping groove 303, so as to avoid the culture dish 304 from rotating together with the stirring rod 207 when the stirring rod 207 rotates.

[0028] Working principle: when the stirring rod 207 is in the auxiliary groove frame 204, the auxiliary groove frame 204 and the limiting frame 301 leave an operation space for the culture dish 304 to move down and put in, the culture dish 304 containing bacteria or yeast and other microorganisms can be stably placed in the limiting frame 301 through the cooperation between the clamping block 305 and the clamping groove 303, then the front door of the nanobody fermentation tank body 1 is closed, and the required fermentation temperature, dissolved oxygen content, PH value and the like of the nanobody are set, in the culture fermentation process, the movable frame 205 and the stirring rod 207 are driven to move down by the electric push rod 206 until the stirring rod 207 reaches the inside of the culture dish 304, then the work shaft 202 and the rotating shaft 203 are driven to rotate forward and backward by the motor 201, so as to drive the stirring rod 207 to rotate forward and backward in the culture dish 304, the bacteria or yeast and other microorganisms in the culture dish 304 are stirred, the fermentation efficiency is improved, after the stirring is finished, the movable frame 205 and the stirring rod 207 are driven to move up by the electric push rod 206, so that the bacteria or yeast and other microorganisms remaining on the surface of the stirring rod 207 are scraped by the auxiliary groove frame 204, the bacteria or yeast and other microorganisms are reduced, and the surface of the stirring rod 207 can be cleaned.

[0029] The embodiments of the utility model disclose the better embodiment, but it is not limited to this, and the ordinary skill of the art, the spirit of the utility model is appreciated to the above-mentioned embodiment, and different extension and change are made, but as long as not departing from the spirit of the utility model, all are in the protection range of the utility model.

Claims

1. An automated stirred nanobody culture fermenter comprising a nanobody fermenter body (1) characterized by, The stirring mechanism (2) is externally provided with a set of limiting assemblies (3) in parallel. The stirring mechanism (2) comprises a motor (201), the motor (201) is arranged at the top of the nanobody fermentation tank body (1), the movable end of the motor (201) is connected with a H-shaped shaft (202), the bottom of the H-shaped shaft (202) is fixedly connected with a rotating shaft (203), a set of auxiliary groove frames (204) are fixedly sleeved outside the rotating shaft (203), a set of movable frames (205) are symmetrically clamped inside the auxiliary groove frames (204), the horizontal surface of the movable frame (205) is connected with an electric push rod (206), and the vertical bottom end of the movable frame (205) is fixedly connected with a stirring rod (207).

2. The automatic stirring nanobody culture fermenter according to claim 1, wherein, A set of the auxiliary groove frames (204) constitute a rotating structure through the rotating shaft (203), the H-shaped shaft (202) and the motor (201), and the vertical part of the movable frame (205) is clamped in the auxiliary groove frame (204).

3. The automatic stirring nanobody culture fermenter according to claim 1, wherein, The movable frame (205) constitutes a lifting structure through the electric push rod (206) and the auxiliary groove frame (204), and the stirring rod (207) at the bottom of the movable frame (205) can be matched and clamped in the auxiliary groove frame (204).

4. The automatic stirring nanobody culture fermenter according to claim 1, wherein, The limiting assembly (3) comprises a limiting frame (301), a set of the limiting frames (301) are sleeved outside the rotating shaft (203) in parallel, a set of connecting blocks (302) are symmetrically and fixedly arranged on the outer surface of the limiting frame (301), a set of clamping grooves (303) are symmetrically and formed on the inner surface of the limiting frame (301), a set of culture dishes (304) are symmetrically clamped in the limiting frame (301), and clamping blocks (305) are fixedly arranged on the surface of the culture dish (304).

5. An automated stirred nanobody culture fermenter according to claim 4, wherein, The limiting frame (301) is fixedly connected with the nanobody fermentation tank body (1) through the connecting block (302), and the rotating shaft (203) can freely rotate in the limiting frame (301).

6. The automatic agitating nanobody culture fermenter according to claim 4, wherein, The culture dish (304) and the clamping block (305) are integrally formed, and the culture dish (304) and the clamping block (305) constitute a clamping structure through the clamping block (305) and the clamping groove (303).

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