Stem cell directional induction automatic liquid changing device

Abstract

The utility model relates to an automatic liquid changing device for directional induction of stem cells, and belongs to the technical field of cell culture devices. The upper part of the culture kettle is provided with a complete culture medium sample introduction pipe, an induction liquid sample introduction pipe and a standby sample introduction pipe, the upper part of the culture kettle is also provided with a gas adapter, the input end of the gas adapter is connected with an oxygen pipe, a nitrogen pipe and a carbon dioxide pipe, and the outlet end of the gas adapter is provided with a gas inlet pipe; the air inlet pipe extends into the lower part of the culture kettle; an emptying pipe is arranged on the side surface of the gas adapter, the tail end of the emptying pipe extends to the upper part of the culture kettle, and an emptying valve is arranged on the emptying pipe; a cell sample outlet pipe is arranged at the bottom of the culture kettle; a waste liquid pipe is arranged at the upper part of the culture kettle; the lower end of the waste liquid pipe extends to the bottom of the culture kettle. The device provided by the utility model can realize mechanical feeding and discharging, is convenient to control, reduces the manual operation process, is convenient and quick, and enables cell liquid replacement to be simpler, more convenient and cleaner.

Description

Technical Field

[0001] This utility model relates to an automatic fluid exchange device for stem cell directional induction, belonging to the technical field of cell culture devices. Background Technology

[0002] Stem cells are undifferentiated, immature cells with the potential to regenerate various tissues and organs, and are medically known as "universal cells." The reason stem cells are called "universal cells" is due to their multipotent differentiation mechanism; stem cells have the ability to differentiate into any type of cell, and through differentiation into different cells, they can form various organs and tissues. Directed differentiation of stem cells refers to the process by which, under specific conditions, stem cells gradually differentiate into one or more specific types of functional cells through gene regulation and intercellular interactions. The induced differentiation of stem cells into functional cells provides more ideas for clinical applications. Currently, in the process of induced differentiation of stem cells into functional cells, cell media replacement is mostly done manually, which is prone to contamination and affects the progress of subsequent applications. Therefore, it is necessary to design a device that can achieve mechanized media replacement, making cell media replacement simpler and cleaner. Summary of the Invention

[0003] To overcome the shortcomings of existing technologies, this invention proposes an automatic fluid exchange device for stem cell directional induction.

[0004] To achieve the above objectives, this utility model employs the following technical solution:

[0005] An automated medium-changing device for targeted stem cell induction includes a device body comprising a culture vessel. The upper part of the culture vessel is equipped with a complete culture medium inlet tube, an induction solution inlet tube, and a spare inlet tube, the outlet ends of which extend above the culture tubes. A gas adapter is also provided on the upper part of the culture vessel. The input end of the gas adapter is connected to an oxygen tube, a nitrogen tube, and a carbon dioxide tube, each equipped with an inlet valve. An inlet pipe is provided at the outlet end of the gas adapter, extending into the lower part of the culture vessel. A vent pipe is provided on the side of the gas adapter, its end extending to the upper part of the culture vessel, and a vent valve is provided on the vent pipe. A cell sample outlet tube is provided at the bottom of the culture vessel. A waste liquid pipe is also provided on the upper part of the culture vessel, equipped with a waste liquid pump, the lower end of which extends to the bottom of the culture vessel.

[0006] Preferably, a transparent observation window is provided on the side wall of the culture vessel.

[0007] Preferably, a sampling tube is also provided at the bottom of the culture vessel, and a sampling valve is provided on the sampling tube.

[0008] Preferably, the upper part of the culture vessel is provided with an upper cover plate, and the upper cover plate is connected to the body of the culture vessel by a flange.

[0009] Preferably, a filter screen is provided at the bottom of the waste liquid pipe.

[0010] Preferably, the upper part of the culture vessel is also provided with a high-pressure rinsing pipeline, and a rinsing nozzle is provided at the outlet of the high-pressure rinsing pipeline.

[0011] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0012] The device provided by this utility model can realize mechanized feeding and discharging, which is easy to control, reduces the manual operation process, and is convenient and quick, making cell culture exchange simpler and cleaner. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model.

[0014] The labels for each figure are as follows: 11 Complete culture medium injection tube, 12 Induction solution injection tube, 13 Spare injection tube, 2 Gas adapter, 21 Oxygen tube, 22 Nitrogen tube, 23 Carbon dioxide tube, 3 Exhaust tube, 4 Waste liquid tube, 5 Transparent observation window, 6 Sampling tube, 7 Cell sample outlet tube, 8 High-pressure flushing tubing, 9 Filter screen. Detailed Implementation

[0015] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0016] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0017] Example 1

[0018] The attached figure shows a specific embodiment of this utility model, such as... Figure 1As shown. The stem cell directed induction automatic medium replacement device provided in this embodiment includes a device body, which is a vessel-type structure. The vessel body and the vessel lid are connected by a flange. There are three sample inlet pipes: a complete culture medium inlet pipe 11, an induction solution inlet pipe 12, and a spare inlet pipe 13. Each of the three inlet pipes is equipped with an inlet pump and a flow meter. The ends of the complete culture medium inlet pipe 11, the induction solution inlet pipe 12, and the spare inlet pipe 13 are all located at the top of the culture vessel. The length of the three inlet pipes inside the culture vessel should be 1-3 cm to prevent excessive insertion and contact with the materials inside the vessel, which could contaminate the fresh culture medium. Different gases need to be introduced during cell culture. Therefore, this embodiment also includes an air intake structure, including an oxygen pipe 21, a nitrogen pipe 22, and a carbon dioxide pipe 23. Each of the three gas pipes is connected to a corresponding gas storage tank, and each gas pipe is equipped with an intake valve. The three intake pipes converge at a gas adapter 2 (commercially available custom-made). An intake pipe is located at the other end of the gas adapter 2, extending to the lower part of the culture vessel. An vent pipe 3 is located next to the gas adapter 2, with its end positioned at the upper part of the culture vessel for easy gas replacement.

[0019] For easy observation, a transparent observation window 5 is provided on the side of the culture vessel. A sampling tube 6 is located below the observation window, and a sampling valve is attached to the sampling tube 6. The outlet of the waste liquid pipe 4 is also located at the top of the culture vessel, with its inlet extending to the bottom of the culture vessel. A filter screen 9 is installed at the inlet of the waste liquid pipe 4. Below the sampling tube 6, a cell sample outlet pipe 7 for cell recovery is provided. After culture, excess culture medium is discharged from the waste liquid pipe 4. Cells are intercepted by the filter screen 9, and the culture medium containing a high concentration of cells is recovered from the cell sample outlet pipe 7. A high-pressure rinsing pipeline 8 is also located at the top of the culture vessel. A pressurized water pump is installed on the high-pressure rinsing pipeline 8. After cell culture and recovery are completed, the water pump of the high-pressure rinsing pipeline 8 can be turned on to rinse the culture vessel. The outlet of the high-pressure rinsing pipeline 8 is a nozzle type, which can effectively increase the cleaning area. If heat preservation is required during cell culture, the entire vessel can be placed in a water bath environment for culture. After the culture is completed, the vessel body is rinsed under high pressure, the vessel lid is removed, and all components inside the vessel body are sterilized by high-temperature steam.

[0020] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the technical solution of the present utility model.

Claims

1. An automated fluid exchange device for targeted stem cell induction, comprising a device body, characterized in that, The device body includes a culture vessel. The upper part of the culture vessel is equipped with a complete culture medium inlet tube, an induction solution inlet tube, and a spare inlet tube. The outlet ends of the complete culture medium inlet tube, the induction solution inlet tube, and the spare inlet tube extend into the upper part of the culture vessel. The upper part of the culture vessel is also equipped with a gas adapter. The input end of the gas adapter is connected to an oxygen tube, a nitrogen tube, and a carbon dioxide tube. Each of the oxygen tube, nitrogen tube, and carbon dioxide tube is equipped with an inlet valve. The outlet end of the gas adapter is equipped with an inlet pipe that extends into the lower part of the culture vessel. A vent pipe is located on the side of the gas adapter, with its end extending into the upper part of the culture vessel. A vent valve is installed on the vent pipe. A cell sample outlet tube is located at the bottom of the culture vessel. A waste liquid pipe is also located at the upper part of the culture vessel, and a waste liquid pump is installed on the waste liquid pipe. The lower end of the waste liquid pipe extends into the bottom of the culture vessel.

2. The automatic fluid exchange device for stem cell directed induction according to claim 1, characterized in that, A transparent observation window is provided on the side wall of the culture vessel.

3. The stem cell directed induction automatic fluid exchange device according to claim 2, characterized in that, A sampling tube is also provided at the bottom of the culture vessel, and a sampling valve is provided on the sampling tube.

4. The stem cell directed induction automatic fluid exchange device according to claim 3, characterized in that, The upper part of the culture vessel is provided with an upper cover plate, which is connected to the body of the culture vessel by a flange.

5. The automatic fluid exchange device for stem cell directed induction according to claim 3 or 4, characterized in that, A filter screen is installed at the bottom of the waste liquid pipe.

6. The automatic fluid exchange device for stem cell directed induction according to claim 5, characterized in that, The upper part of the culture vessel is also equipped with a high-pressure rinsing pipeline, and a rinsing nozzle is installed at the outlet of the high-pressure rinsing pipeline.

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