Embryo unfreezing plate

Abstract

The utility model relates to the technical field of embryo unfreezing plates, in particular to an embryo unfreezing plate which comprises an unfreezing plate body made of high-molecular low-temperature-resistant materials, a first small round hole is formed in the top of the unfreezing plate body and used for containing TS liquid in a vitrification unfreezing set, and a second small round hole is formed in the top of the unfreezing plate body and used for containing the TS liquid in the vitrification unfreezing set. The top of the thawing plate body is provided with five second round small holes in a linear array, the second round small holes are used for placing liquid drops of freezing liquid or thawing liquid used in the vitrification thawing process, and the radius of the first round small holes is larger than that of the second round small holes. The unfreezing plate body can be driven to move by manually taking the circular connecting rod, the unfreezing plate body is convenient to move and transfer, the unfreezing plate body does not need to be manually contacted in the whole process, and the situation that the unfreezing plate body is polluted due to manual contact, and finally the quality of embryos is affected is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of embryo thawing plate, specifically is a kind of embryo thawing plate. BACKGROUND

[0002] In modern assisted reproductive technology, vitrification freezing is the core technology for preserving embryos, oocytes and sperm, and embryo thawing requires the assistance of tools such as embryo thawing plates.

[0003] In the prior art, such as patent application number "CN201821982936.9" proposes a kind of embryo vitrification freezing dish: including freezing dish body and the freezing dish cover used in cooperation with freezing dish body, the inner cavity of freezing dish body is provided with area partition plate;The inner cavity of freezing dish body is divided into cleaning part, balance part, freezing part and placement part by area partition plate.

[0004] However, in the prior art, the activity and transfer of the embryo thawing plate need to manually take the embryo thawing plate, manually take the embryo thawing plate, and the hand contacts the embryo thawing plate, which may contaminate the embryo thawing plate and ultimately affect the quality of the embryo. UTILITY MODEL CONTENT

[0005] The utility model aims to provide a kind of embryo thawing plate, to solve the problems raised in the above background technology.

[0006] The purpose of the utility model can be realized by the following technical solutions:

[0007] An embryo thawing plate, comprising a thawing plate body made of a high-molecular low-temperature resistant material, a first circular hole is formed in the top of the thawing plate body, the first circular hole is used to place the TS liquid in the glass thawing set, five second circular holes are formed in the top of the thawing plate body in a linear array, the second circular holes are used to place the droplets of freezing liquid or thawing liquid used in the glass freezing and thawing process, the radius of the first circular hole is greater than the radius of the second circular hole.

[0008] The bottom of the thawing plate body is fixedly connected with two positioning brackets, a positioning groove is formed in the positioning bracket, a circular link is arranged at the bottom of the thawing plate body, the two ends of the circular link are fixedly connected with positioning plates, a horizontally movable positioning lug is arranged on one side of the positioning plate, and the positioning lug and the positioning groove are matched.

[0009] Preferably, a sample loading rod loading platform is arranged on the top of the thawing plate body, and flexible protective pads are fixedly connected around the outer surface of the thawing plate body.

[0010] Preferably, the circular connecting rod has a cavity inside, a fixed sleeve is fixedly connected to the inner surface of the circular connecting rod, and a movable shaft is slidably connected to the fixed sleeve; the positioning plate has a cavity inside, and one end of the movable shaft extends into the cavity of the positioning plate.

[0011] Preferably, the outer surface of the movable shaft is fixedly connected with a positioning ring, a tension spring is fixedly connected between the positioning ring and the fixed sleeve, and the tension spring is sleeved on the outer surface of the movable shaft.

[0012] Preferably, the circular connecting rod is provided with a movable notch on one side, one end of the movable shaft penetrates through the movable notch and is rotatably connected with a connecting plate, and one end of the connecting plate is rotatably connected with a control pressing plate.

[0013] Preferably, one end of the movable shaft inside the positioning plate is fixedly connected with a connecting shaft, and one end of the connecting shaft is fixedly connected with a positioning protrusion, so as to drive the positioning protrusion to move together.

[0014] The utility model discloses beneficial effects:

[0015] The utility model discloses a positioning protrusion is clamped into the inboard of positioning support, makes the manual taking circular connecting rod, can drive thawing plate body to be active, the activity and the removal of thawing plate body are convenient, in the whole process, need not manual contact thawing plate body, avoid thawing plate body because manual contact produces the pollution, finally influence the quality of embryo, press control pressing plate simultaneously, can separate positioning protrusion and positioning support, take down circular connecting rod and positioning plate from thawing plate body, convenient individual to circular connecting rod and positioning plate carry out the sterilization. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, obviously, for the ordinary skilled person in the art, without paying the creative labor, still can obtain other drawings according to these drawings;

[0017] Figure 1 It is the whole structure schematic diagram of the utility model;

[0018] Figure 2 It is the structure schematic diagram of the utility model Figure 1 thawing plate body bottom in the embodiment;

[0019] Figure 3 It is the rear view of thawing plate body in the utility model Figure 2

[0020] Figure 4 It is the structure schematic diagram of the utility model Figure 2 circular connecting rod and positioning plate inside in the embodiment.

[0021] ​The reference signs in the drawings are as follows:

[0022] 1. thawing plate body; 101. first circular hole; 102. second circular hole; 103. sample carrier loading platform; 104. flexible protective pad; 2. positioning bracket; 3. circular connecting rod; 301. movable notch; 4. positioning plate; 5. positioning block; 6. fixed sleeve; 7. movable shaft; 8. positioning ring; 9. tension spring; 10. connecting plate; 11. control pressing plate; 12. connecting shaft. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0024] As Figure 1 An embryo thawing plate includes a thawing plate body 1 made of a high-molecular low-temperature-resistant material, which can stably operate between -196°C and 37°C without deformation or decomposition, and the surface thereof is treated with hydrophilicity to help stable distribution of liquid drops. The thawing plate body 1 is sterilized by gamma rays and meets the sterile and low-endotoxin standards (<0.5 EU / set) of the Pharmacopoeia.

[0025] A first circular hole 101 is formed in the top of the thawing plate body 1 and is used to place TS liquid in a vitrification thawing kit. Five second circular holes 102 in linear array are formed in the top of the thawing plate body 1 and are used to place liquid drops of freezing or thawing liquid used in the vitrification freezing and thawing process. The radius of the first circular hole 101 is greater than that of the second circular hole 102.

[0026] The first circular hole 101 is a TS hole, and the five second circular holes are respectively DS1 / ES1 holes, DS2 / ES2 holes, DS3 / ES3 holes, WS1 / VS1 holes and WS2 / VS2 holes.

[0027] The diameter of the second circular hole 102 is 2-5 mm, and the depth is 1-3 mm. The diameter of the first circular hole 101 is 10-15 mm, and the depth is 5-8 mm.

[0028] The freezing operation is as follows:

[0029] Preparation: According to the experimental procedure, the freezing liquid is respectively added to the first circular hole 101 and the second circular hole 102.

[0030] Operation: Place the gametes or embryos in the droplets in the first circular well 101, the second circular well 102, and observe the state changes in the droplets.

[0031] Freezing: Finally, quickly transfer the gametes or embryos to the freezing carrier and then place them in liquid nitrogen.

[0032] Thawing operation is as follows:

[0033] Thawing solution preparation: Add the thawing solution to the first circular well 101 (TS well), and add the dilution solution to the second circular well 102 (WS well and DS well).

[0034] Operation flow:

[0035] Quickly place the sample taken out of liquid nitrogen into the TS well for initial thawing.

[0036] Transfer to the DS and WS droplets in turn to complete dilution and equilibration.

[0037] As Figure 1 and Figure 2 , the bottom of the thawing plate body 1 is fixedly connected with two positioning supports 2, the positioning supports 2 are provided with positioning notches, the bottom of the thawing plate body 1 is provided with a circular connecting rod 3, both ends of the circular connecting rod 3 are fixedly connected with positioning plates 4, one side of the positioning plate 4 is provided with a horizontally movable positioning lug 5, and the positioning lug 5 is matched with the positioning notches.

[0038] As Figure 1 , the top of the thawing plate body 1 is provided with a sample carrier rod loading table 103, and flexible protective pads 104 are fixedly connected around the outer surface of the thawing plate body 1, which can provide flexible protection for the thawing plate body 1.

[0039] As Figure 1 , the circular connecting rod 3 has a cavity, a fixed sleeve 6 is fixedly connected to the inner surface of the circular connecting rod 3, the fixed sleeve 6 is slidably connected with a movable shaft 7, so that the movable shaft 7 can move in the circular connecting rod 3, the positioning plate 4 has a cavity, one end of the movable shaft 7 extends into the cavity of the positioning plate 4, and the positioning plate 4 has an opening for the movable shaft 7 to pass through.

[0040] As Figure 1 , the outer surface of the movable shaft 7 is fixedly connected with a positioning ring 8, a tension spring 9 is fixedly connected between the positioning ring 8 and the fixed sleeve 6, the tension spring 9 has a pulling force on the positioning ring 8 in the direction of the fixed sleeve 6, and the tension spring 9 is sleeved on the outer surface of the movable shaft 7.

[0041] As Figure 3 and Figure 4The side of the circular connecting rod 3 is provided with a movable notch 301, one end of the movable shaft 7 penetrates through the movable notch 301 and is rotationally connected with the connecting plate 10, one end of the connecting plate 10 is rotationally connected with the control pressing plate 11, and the movable notch 301 facilitates the movement of the movable shaft 7.

[0042] As Figure 3 and Figure 4 One end of the movable shaft 7 is fixedly connected with the connecting shaft 12 inside the positioning plate 4, one end of the connecting shaft 12 is fixedly connected with the positioning block 5, the positioning block 5 penetrates through one side of the positioning plate 4 and is clamped into the inside of the positioning support 2, and the positioning block 5 drives the positioning support 2 to move together.

[0043] The working principle of the embryo thawing plate is as follows:

[0044] When the thawing plate body 1 needs to be moved, the positioning block 5 is clamped in the inside of the positioning support 2, at this time, the circular connecting rod 3 is manually taken, the circular connecting rod 3 drives the positioning plate 4 to move, the positioning plate 4 drives the positioning support 2 to move, the positioning support 2 drives the thawing plate body 1 to move, and the thawing plate body 1 is conveniently moved to the microscope or other thawing equipment;

[0045] The control pressing plate 11 is pressed, the control pressing plate 11 drives the movable shaft 7 to move through the connecting plate 10, one end of the movable shaft 7 drives the connecting shaft 12 to move, the connecting shaft 12 drives the positioning block 5 to move into the positioning plate 4, and the positioning block 5 is separated from the positioning support 2, at this time, the thawing plate body 1 can be taken off, and the circular connecting rod 3 and the positioning plate 4 can be sterilized separately.

[0046] Conversely, the control pressing plate 11 is loosened, the positioning ring 8 is driven by the tension spring 9, the positioning ring 8 drives the movable shaft 7, the connecting shaft 12 and the positioning block 5 to move, the positioning block 5 is clamped into the inside of the positioning support 2, at this time, the thawing plate body 1 can be driven by the circular connecting rod 3 to move.

[0047] Compared with the related art, the embryo thawing plate has the following beneficial effects:

[0048] The positioning block 5 is clamped into the inside of the positioning support 2, the circular connecting rod 3 is manually taken, the thawing plate body 1 is driven to move, the movement and movement of the thawing plate body 1 are facilitated, the thawing plate body 1 does not need to be manually contacted in the whole process, the thawing plate body 1 is prevented from being polluted due to manual contact, the quality of the embryo is finally affected, the control pressing plate 11 is pressed, the positioning block 5 and the positioning support 2 are separated, the circular connecting rod 3 and the positioning plate 4 are taken off from the thawing plate body 1, and the circular connecting rod 3 and the positioning plate 4 are sterilized separately.

[0049] The basic principle, main features and advantages of the present application are shown and described above. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application.

Claims

1. An embryo thawing plate comprising a thawing plate body (1) made of a high-molecular cryogenic material, characterized in that, The top of the thawing plate plate body (1) is provided with a first circular hole (101) for placing TS liquid in the vitrification thawing kit, and the top of the thawing plate plate body (1) is provided with five second circular holes (102) arranged in a linear array for placing droplets of freezing liquid or thawing liquid used in the vitrification freezing and thawing process, and the radius of the first circular hole (101) is greater than the radius of the second circular hole (102). The bottom of the thawing plate plate body (1) is fixedly connected with two positioning supports (2), the positioning supports (2) are provided with positioning notches, the bottom of the thawing plate plate body (1) is provided with a circular connecting rod (3), both ends of the circular connecting rod (3) are fixedly connected with positioning plates (4), one side of the positioning plates (4) is provided with horizontally movable positioning lugs (5), and the positioning lugs (5) and the positioning notches are matched.

2. An embryo thawing plate according to claim 1, wherein The top of the thawing plate plate body (1) is provided with a sample carrier loading table (103), and the outer surface of the thawing plate plate body (1) is fixedly connected with flexible protective pads (104) around.

3. The embryo thawing plate of claim 1, wherein The inside of the circular connecting rod (3) has a cavity, the inner surface of the circular connecting rod (3) is fixedly connected with a fixed sleeve (6), the fixed sleeve (6) is slidably connected with a movable shaft (7), the inside of the positioning plate (4) is provided with a cavity, and one end of the movable shaft (7) extends into the inside of the positioning plate (4).

4. An embryo thawing plate according to claim 3, wherein, The outer surface of the movable shaft (7) is fixedly connected with a positioning ring (8), the positioning ring (8) and the fixed sleeve (6) are fixedly connected with a tension spring (9), and the tension spring (9) is sleeved on the outer surface of the movable shaft (7).

5. An embryo thawing plate according to claim 3, wherein One side of the circular connecting rod (3) is provided with a movable notch (301), one end of the movable shaft (7) penetrates through the movable notch (301) and is rotatably connected with a connecting plate (10), and one end of the connecting plate (10) is rotatably connected with a control pressing plate (11).

6. An embryo thawing plate according to claim 5, wherein, One end of the movable shaft (7) inside the positioning plate (4) is fixedly connected with a connecting shaft (12), one end of the connecting shaft (12) is fixedly connected with the positioning lug (5), and the connecting shaft (12) is used to drive the positioning lug (5) to move together.

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