Nucleic acid purification device

Abstract

The utility model relates to the technical field of nucleic acid purification, in particular to a nucleic acid purification device which comprises a waste liquid pipe, a nucleic acid purification column and a filter pipe, a blocking ring is fixedly connected inside the waste liquid pipe and located below the nucleic acid purification column, and a liquid leakage opening is formed in the blocking ring. A sealing plug capable of moving up and down is arranged in the waste liquid pipe and located below the liquid leakage opening, and the sealing plug is designed to be a table body. The bottom of the waste liquid pipe is sleeved with a bearing pipe, the bottom of the inner wall of the waste liquid pipe is fixedly connected with a guide sleeve, the bottom of the waste liquid pipe is fixedly connected with a protruding block, the bearing pipe is taken down, the adjusting rod can be rotated to drive the sealing plug to move up and down, and after waste liquid completely falls below the blocking ring, the sealing plug can move upwards to seal the liquid leakage opening; the pollution to the nucleic acid purification column caused by waste liquid backflow due to careless dumping or excessive inclination of the device in the transferring process is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid purification technical field, concretely is a nucleic acid purification device. BACKGROUND

[0002] The main purpose of nucleic acid purification is to separate DNA and / or RNA from the sample and remove all other cellular and extracellular components to ensure the purity and integrity of nucleic acids.

[0003] In the prior art, such as patent application number "CN201821608314.X" proposes a nucleic acid purification device, including nucleic acid purification column, the sample adding port of nucleic acid purification column is equipped with sealing plug, the nucleic acid purification device still includes waste liquid pipe and filter pipe, the structure of waste liquid pipe is compatible with the structure of nucleic acid purification column, so that nucleic acid purification column is sleeved in waste liquid pipe.

[0004] But in the above patent application, the waste liquid produced by nucleic acid purification will finally enter the inside of the waste liquid pipe, and when the transfer device is moved, if the device is accidentally tilted or excessively inclined, it will cause the backflow of nuclear waste liquid and contaminate the nucleic acid purification column. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a nucleic acid purification device to solve the problems proposed in the above background.

[0006] The purpose of the utility model can be realized by the following technical scheme:

[0007] A nucleic acid purification device, including waste liquid pipe, nucleic acid purification column and filter pipe, the inside of waste liquid pipe and below nucleic acid purification column are fixedly connected with blocking ring, the blocking ring is provided with liquid leakage opening, the inside of waste liquid pipe and below liquid leakage opening are provided with seal plug that can move up and down, the seal plug is designed as a table body;

[0008] The bottom of waste liquid pipe is sleeved with bearing pipe, the bottom of waste liquid pipe inner wall is fixedly connected with guide sleeve, the bottom of waste liquid pipe is fixedly connected with protruding block, the protruding block is threadedly connected with adjusting rod, the top of adjusting rod penetrates waste liquid pipe, guide sleeve and extends to the inside of waste liquid pipe in proper order, the top of adjusting rod and seal plug are fixedly connected and used to drive seal plug to move up and down.

[0009] Preferably, the waste liquid pipe is sleeved on the outer surface of the nucleic acid purification column, and the nucleic acid purification column is sleeved on the outer surface of the filter pipe.

[0010] Preferably, the bottom of the seal plug is fixedly connected with a protection tube, the protection tube is sleeved on the outer surface of the guide sleeve, and the outer surface of the guide sleeve and below the protection tube is fixedly connected with a limiting ring.

[0011] Preferably, the inside of the waste liquid pipe is fixedly connected with a flow guide hopper, the inner surface and the outer surface of the flow guide hopper are designed as curved surfaces, so as to reduce waste liquid backflow.

[0012] Preferably, the outer surface of the waste liquid pipe is provided with an annular positioning groove, and the inner surface of the bearing pipe is fixedly connected with a rubber positioning ring matched with the annular positioning groove.

[0013] Preferably, the bottom of the filter pipe is provided with a filter hole, the bottom of the inner wall of the filter pipe is provided with a filter layer, the bottom of the inner wall of the nucleic acid purification column is fixedly connected with a nucleic acid adsorptive porous membrane, and the bottom of the nucleic acid purification column is fixedly connected with a discharge pipe.

[0014] The device has the advantages that:

[0015] The device has the advantages that: BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the drawings needed in the embodiments or the prior art description will be briefly introduced as follows, and obviously, other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0017] Figure 1 is a schematic diagram of the overall structure of the present application;

[0018] Figure 2 is a schematic diagram of the overall structure of the present application; Figure 1 is a schematic diagram of the connection of the bearing pipe and the waste liquid pipe in the present application;

[0019] Figure 3 is a schematic diagram of the connection of the bearing pipe and the waste liquid pipe in the present application; Figure 2 is a sectional view of the bearing pipe in the present application;

[0020] Figure 4 is a sectional view of the bearing pipe in the present application; Figure 3 is an enlarged view of A in the present application.

[0021] The reference signs in the drawings are as follows:

[0022] 1, waste pipe; 2, nucleic acid purification column; 201, nucleic acid adsorption porous membrane; 202, discharge port; 3, filter tube; 301, filter hole; 302, filter layer; 4, blocking ring; 401, liquid leakage opening; 5, sealing plug; 6, protruding block; 7, adjusting rod; 701, limiting ring; 8, protection tube; 9, flow guide hopper; 10, annular positioning groove; 11, rubber positioning ring; 12, bearing tube; 13, guide sleeve. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0024] As Figure 1 , Figure 2 and Figure 3 , a nucleic acid purification device comprises a waste pipe 1, a nucleic acid purification column 2 and a filter tube 3, the waste pipe 1, the nucleic acid purification column 2 and the filter tube 3 are sequentially arranged from outside to inside, the inside of the waste pipe 1 and below the nucleic acid purification column 2 are fixedly connected with a blocking ring 4, the blocking ring 4 is provided with a liquid leakage opening 401, the inside of the waste pipe 1 and below the liquid leakage opening 401 are provided with a sealing plug 5 which can move up and down, the sealing plug 5 is designed as a table body, if the sealing plug 5 moves up, the liquid leakage opening 401 can be closed;

[0025] As Figure 1 , Figure 2 and Figure 3 , the bottom of the waste pipe 1 is sleeved with a bearing tube 12, the bottom of the inner wall of the waste pipe 1 is fixedly connected with a guide sleeve 13, the bottom of the waste pipe 1 is fixedly connected with a protruding block 6, the protruding block 6 is threadedly connected with an adjusting rod 7, the top of the adjusting rod 7 penetrates the waste pipe 1, the guide sleeve 13 in sequence and extends into the inside of the waste pipe 1, on one hand, the guide sleeve 13 can avoid the deviation of the adjusting rod 7, on the other hand, the guide sleeve 13 can avoid the waste liquid passing through the opening at the bottom of the adjusting rod 7 and the waste pipe 1, the bottom of the adjusting rod 7 has a handle, which facilitates the manual rotation of the adjusting rod 7, the top of the adjusting rod 7 is fixedly connected with the sealing plug 5, for driving the sealing plug 5 to move up and down.

[0026] As Figure 1 , Figure 2 and Figure 3The waste liquid pipe 1 is sleeved on the outer surface of the nucleic acid purification column 2, the nucleic acid purification column 2 is sleeved on the outer surface of the filter pipe 3, and the edges of the top of the nucleic acid purification column 2 and the filter pipe 3 are all annular protrusions, so that the nucleic acid purification column 2 cannot completely fall into the waste liquid pipe 1, and the filter pipe 3 cannot completely fall into the nucleic acid purification column 2.

[0027] As Figure 1 , Figure 2 and Figure 3 The bottom of the sealing plug 5 is fixedly connected with a protection pipe 8, the protection pipe 8 is sleeved on the outer surface of the guide sleeve 13, the protection pipe 8 can protect the opening at the top of the guide sleeve 13, the outer surface of the guide sleeve 13 and below the protection pipe 8 is fixedly connected with a limiting ring 701, and the limiting ring 701 can limit the maximum distance of the downward movement of the protection pipe 8 and the sealing plug 5.

[0028] As Figure 1 , Figure 2 and Figure 3 The inner surface and the outer surface of the deflector 9 are both designed as curved surfaces, so that the deflector 9 can reduce the backflow of waste liquid when the waste liquid pipe 1 is poured.

[0029] As Figure 3 and Figure 4 An annular positioning groove 10 is formed in the outer surface of the waste liquid pipe 1, and a rubber positioning ring 11 is fixedly connected to the inner surface of the bearing pipe 12; the rubber positioning ring 11 is matched with the annular positioning groove 10; the elasticity of the rubber positioning ring 11 can make the rubber positioning ring 11 be clamped into the annular positioning groove 10 when the bearing pipe 12 is sleeved on the bottom of the waste liquid pipe 1, so that the positioning between the bearing pipe 12 and the waste liquid pipe 1 is realized; otherwise, the waste liquid pipe 1 can be separated from the bearing pipe 12 by being pulled upward.

[0030] As Figure 1 A filter hole 301 is formed in the bottom of the filter pipe 3, a filter layer 302 is arranged on the inner wall of the filter pipe 3, the filter layer 302 is made of loose cotton-like material, a nucleic acid adsorption porous membrane 201 is fixedly connected to the inner wall of the nucleic acid purification column 2, and a discharge pipe 202 is fixedly connected to the bottom of the nucleic acid purification column 2; so that the filter layer 302 can filter impurities when nucleic acid purification is carried out, and the nucleic acid adsorption porous membrane 201 can adsorb DNA in filtrate in the nucleic acid purification column 2.

[0031] The working principle of the nucleic acid purification device is as follows:

[0032] When the waste liquid falls into the position below the blocking ring 4 in the waste liquid pipe 1 through the filtering layer 302 and the nucleic acid adsorption porous membrane 201 and passes through the liquid leakage opening 401, the bearing pipe 12 and the waste liquid pipe 1 can be separated, and the adjusting rod 7 is manually rotated to drive the sealing plug 5 to move upwards, and the sealing plug 5 seals the liquid leakage opening 401;

[0033] When the device is poured or excessively inclined, the waste liquid cannot pass through the blocking ring 4 due to the sealing of the liquid leakage opening 401 by the sealing plug 5, and the waste liquid reflux and the nucleic acid purification column 2 are avoided, and the waste liquid cannot contact the nucleic acid purification column 2 to cause the nucleic acid purification column 2 to be polluted.

[0034] Compared with the related art, the nucleic acid purification device has the following beneficial effects:

[0035] The nucleic acid purification device has the following beneficial effects:

[0036] The basic principle, main features and advantages of the nucleic acid purification device are shown and described above. It should be understood by those skilled in the art that the nucleic acid purification device is not limited to the above embodiments, and the above embodiments and descriptions in the specification are only to illustrate the principle of the nucleic acid purification device. Without departing from the spirit and scope of the nucleic acid purification device, various changes and improvements can be made to the nucleic acid purification device, and these changes and improvements all fall within the scope of the claimed nucleic acid purification device.

Claims

1. A nucleic acid purification device comprising a waste tube (1), a nucleic acid purification column (2) and a filter tube (3), characterized in that, The inside of the waste liquid pipe (1) is fixedly connected with a blocking ring (4) below the nucleic acid purification column (2), the blocking ring (4) is provided with a liquid leakage opening (401), the inside of the waste liquid pipe (1) is provided with a seal plug (5) which can move up and down below the liquid leakage opening (401), and the seal plug (5) is designed as a table body. The bottom of the waste liquid pipe (1) is sleeved with a bearing pipe (12), the bottom of the inner wall of the waste liquid pipe (1) is fixedly connected with a guide sleeve (13), the bottom of the waste liquid pipe (1) is fixedly connected with a protruding block (6), the protruding block (6) is threadedly connected with an adjusting rod (7), the top of the adjusting rod (7) penetrates the waste liquid pipe (1), the guide sleeve (13) and extends into the inside of the waste liquid pipe (1) in sequence, and the top of the adjusting rod (7) is fixedly connected with the seal plug (5) and is used for driving the seal plug (5) to move up and down.

2. The nucleic acid purification device of claim 1, wherein, The waste liquid pipe (1) is sleeved on the outer surface of the nucleic acid purification column (2), and the nucleic acid purification column (2) is sleeved on the outer surface of the filter pipe (3).

3. The nucleic acid purification device of claim 1, wherein, The bottom of the seal plug (5) is fixedly connected with a protection pipe (8), the protection pipe (8) is sleeved on the outer surface of the guide sleeve (13), and the outer surface of the guide sleeve (13) and below the protection pipe (8) is fixedly connected with a limiting ring (701).

4. The nucleic acid purification device of claim 1, wherein, The inside of the waste liquid pipe (1) is fixedly connected with a flow guide hopper (9), the inner surface and the outer surface of the flow guide hopper (9) are designed as curved surfaces, and the flow guide hopper (9) is used for reducing waste liquid backflow.

5. The nucleic acid purification device of claim 1, wherein, The outer surface of the waste liquid pipe (1) is provided with an annular positioning groove (10), the inner surface of the bearing pipe (12) is fixedly connected with a rubber positioning ring (11), and the rubber positioning ring (11) and the annular positioning groove (10) are matched.

6. The nucleic acid purification device of claim 1, wherein, The bottom of the filter pipe (3) is provided with a filter hole (301), the bottom of the inner wall of the filter pipe (3) is provided with a filter layer (302), the bottom of the inner wall of the nucleic acid purification column (2) is fixedly connected with a nucleic acid adsorption porous membrane (201), and the bottom of the nucleic acid purification column (2) is fixedly connected with a discharge pipe (202).

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