Filtering and extracting equipment for stem cell exosome

Abstract

The utility model relates to filtering and extracting equipment for stem cell exosomes, which belongs to the technical field of filtering and extracting of the stem cell exosomes and comprises a storage tank, and a supporting hopper connected through a driving component is arranged in the storage tank. The cell sap discharging device has the advantages that the arranged discharging pipe is used for pouring out cell sap, when the discharging pipe is connected, the bolt plug is in threaded connection with the interior of the discharging pipe through the hand wheel block, and then the locking screw in the locking block at the upper end of the hand wheel block is rotated to be locked and installed with the threaded hole in the positioning ring on the outer wall of the discharging pipe; the connection stability of the bolt plug is ensured, the connection stability between the bolt plug and the discharge pipe is remarkably enhanced through a double-locking mechanism, it is ensured that the bolt plug cannot fall off due to vibration or centrifugal force in the centrifugal process, and the stable bolt plug connection can effectively prevent cell sap from leaking from the discharge pipe in the centrifugal process; and the bolt plug is stably connected, so that potential safety hazards caused by falling during centrifugal rotation are avoided.

Description

Technical Field

[0001] This utility model relates to the field of stem cell exosome filtration and extraction technology, specifically a stem cell exosome filtration and extraction device. Background Technology

[0002] Stem cell exosomes are extracellular secretions released by stem cells. They mainly contain bioactive molecules such as proteins, nucleic acids, and lipids. These biomolecules can be transferred between cells, thereby regulating physiological processes such as cell growth, differentiation, and apoptosis. They have a wide range of biological functions and medical applications.

[0003] Reference patent (CN202420334126.1) relates to a stem cell exosome filtration and extraction device, including a storage tank. A rotating rail is welded to the top of the inner side of the storage tank. A support bucket is embedded in the inner side of the rotating rail via a bearing. Several separation tanks are embedded in the outer surface of the top of the support bucket. A top cover is screwed to the top of the separation tank. A downward screw groove is opened in the middle of the inner side of the top cover. An extended rotating rod is embedded through the middle inner side of the top cover. This solution reduces the area of ​​cell fluid adhesion by storing stem cells in separate separation tanks. By setting an extended rotating rod that can move spirally at the top of the separation tank, the extended rotating rod drives the filtration mechanism to separate the centrifuged cell shell and cell fluid inside the separation tank. It can reduce the cleaning conditions without affecting the separation speed and has a more convenient cleaning function compared with the prior art.

[0004] Based on the aforementioned patent, the discharge pipe outlet is blocked by a rubber stopper, which pours out the cell fluid. However, when the discharge pipe is connected by a rubber stopper, it is easy for the rubber stopper to fall off during the centrifugal rotation of the separation tank, causing the cell fluid to flow out. In addition, the stability and sealing of the filter screen during installation are difficult to guarantee, and there is no effective obstruction at the edge, which easily causes the cell fluid to overflow at the edge when it is removed. Finally, during cleaning, cells or impurities are easily deposited at the bottom and inner wall of the separation tank, and there is no effective scraping mechanism, which makes internal cleaning inconvenient. In response to the above situations, we have launched a stem cell exosome filtration and extraction device. Utility Model Content

[0005] The purpose of this invention is to provide a stem cell exosome filtration and extraction device to solve the problems mentioned in the background art, which involve a rubber stopper blocking the discharge port of the discharge tube to pour out the cell fluid. However, when the discharge tube is connected by a rubber stopper, the rubber stopper is prone to falling off during centrifugal rotation of the separation tank, causing the cell fluid to flow out. Furthermore, the stability and sealing of the filter screen during installation are difficult to guarantee, and there is no effective obstruction at the edges, which easily causes the cell fluid to overflow at the edges when it is removed. Finally, during cleaning, cells or impurities easily accumulate at the bottom and inner wall of the separation tank, and there is no effective scraping mechanism, which makes internal cleaning inconvenient.

[0006] The technical solution of this utility model is:

[0007] The system includes a storage tank, the interior of which is provided with a support bucket connected by a drive assembly, the inner wall of which is fixed with an installation block, and the interior of which is provided with a separation tank connected by a locking assembly.

[0008] The upper end of the separator is provided with a connecting rod connected by a threaded assembly. A filter plate is fixed to the lower end of the connecting rod. A cylinder is fixed to the outer wall of the filter plate. A support ring is fixed to the inner wall of the separator. A rubber ring is fixed to the upper end face of the support ring. The lower end of the separator is provided with a first scraper connected by a rotating assembly. A second scraper is fixed to the end of the first scraper. A discharge pipe is fixed through the side of the separator. A bolt plug is threaded into the discharge pipe. A handwheel block is fixed to one end of the bolt plug. A locking assembly for fixing is provided at the upper end of the handwheel block.

[0009] Furthermore, the threaded assembly includes a bolt block with an internal threaded connection at the upper end of the separator tank, a handle fixed to the upper end of the bolt block, and a connecting rod fixedly connected to the lower end of the bolt block.

[0010] The filter plate and separator are installed and disassembled by manually rotating the bolt block, facilitating equipment maintenance and cleaning. The filter plate and separator are fitted to the rubber ring surface on the upper end of the support ring, providing support during installation and ensuring the stability of the internal filter plate. A stable filter plate can effectively prevent displacement or damage caused by vibration or pressure changes. The rubber ring ensures the seal between the filter plate and the separator, and the filtered cell liquid after centrifugation is filtered inside the filter plate and separator, reducing cell liquid overflow at the edges during removal. This reduction in overflow not only reduces cell liquid loss but also avoids the risk of contamination caused by overflow, thereby improving the reliability and safety of the entire filtration and extraction process.

[0011] Furthermore, the rotating assembly includes a drive rod that runs through the lower end of the separation tank, and the first scraper is fixedly connected to the output end of the drive rod.

[0012] The bottom drive rod of the separation tank is driven by a motor, which in turn drives the first scraper to rotate and adhere to the bottom surface of the separation tank. This effectively scrapes up the cells or impurities deposited at the bottom, allowing them to re-enter the solution for centrifugation, thereby improving separation efficiency. Then, the second scraper rotates and adheres to the inner wall of the separation tank, removing cells or impurities attached to the inner wall and ensuring the smooth progress of the centrifugation process, further improving separation efficiency. The mechanical scraping action can more thoroughly remove residues inside the separation tank, enhancing the cleaning effect. The scraper can cover the bottom and inner wall of the separation tank, ensuring thorough cleaning without dead corners and avoiding the impact of residues on subsequent experiments.

[0013] This invention provides an improved filtration and extraction device for stem cell exosomes, which has the following improvements and advantages compared with the prior art:

[0014] Firstly, this invention allows for easy installation and disassembly of the separator by manually rotating the bolt block, facilitating equipment maintenance and cleaning. The filter plate and cylinder are fitted onto the rubber ring surface on the upper end of the support ring, providing support during installation and ensuring the stability of the internal filter plate. A stable filter plate effectively prevents displacement or damage caused by vibration or pressure changes. The rubber ring ensures a tight seal between the filter plate and the separator, and the filtered cell liquid after centrifugation is contained within the filter plate and cylinder, reducing overflow at the edges during removal. This reduction in overflow not only minimizes cell liquid loss but also avoids the risk of contamination, thereby improving the reliability and safety of the entire filtration and extraction process.

[0015] Secondly, in this invention, the bottom drive rod of the separation tank is driven by a motor, which in turn drives the first scraper to rotate and adhere to the bottom surface of the separation tank. This effectively scrapes up the cells or impurities deposited at the bottom, allowing them to re-enter the solution for centrifugation, thereby improving separation efficiency. Then, the second scraper rotates and adheres to the inner wall of the separation tank, removing cells or impurities attached to the inner wall and ensuring the smooth progress of the centrifugation process, further improving separation efficiency. The mechanical scraping action can more thoroughly remove residues inside the separation tank, enhancing the cleaning effect. The scraper can cover the bottom and inner wall of the separation tank, ensuring thorough cleaning without dead corners and avoiding the impact of residues on subsequent experiments.

[0016] Thirdly, in this invention, the discharge tube is used to pour out the cell fluid. When connecting the discharge tube, the bolt plug is threaded into the inside of the discharge tube via a handwheel block. Then, the locking screw inside the locking block at the upper end of the handwheel block is rotated to lock the bolt plug in place with the threaded hole inside the positioning ring on the outer wall of the discharge tube. This ensures the stability of the bolt plug during connection. The double locking mechanism significantly enhances the connection stability between the bolt plug and the discharge tube, ensuring that the bolt plug will not fall off due to vibration or centrifugal force during centrifugation. The stable bolt plug connection effectively prevents cell fluid from leaking from the discharge tube during centrifugation. The secure connection of the bolt plug avoids potential safety hazards caused by it falling off during centrifugation. Attached Figure Description

[0017] The present invention will be further explained below with reference to the accompanying drawings and embodiments:

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the internal structure of the storage tank of this utility model;

[0020] Figure 3 This is a schematic diagram of the connection structure of the separator tank of this utility model;

[0021] Figure 4 This is a schematic cross-sectional view of the separation tank of this utility model;

[0022] Figure 5 This is a schematic diagram of the bolt plug connection structure of this utility model.

[0023] Explanation of reference numerals in the attached drawings: 1. Storage tank; 2. Cover; 3. Drive shaft; 301. Support bucket; 302. Slip ring; 303. Positioning slide rail; 4. Mounting block; 401. Separation tank; 402. Limiting plate; 5. Bolt block; 501. Handle; 502. Connecting rod; 503. Filter plate; 504. Cylinder; 6. Support ring; 601. Rubber ring; 7. Drive rod; 701. First scraper; 702. Second scraper; 8. Discharge pipe; 801. Positioning ring; 802. Threaded hole; 803. Bolt plug; 804. Handwheel block; 805. Locking block. Detailed Implementation

[0024] The following will be combined with the appendix Figures 1 to 5 This utility model will be described in detail, and the technical solutions in the embodiments of this utility model will be clearly and completely described. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0025] This invention provides an improved filtration and extraction device for stem cell exosomes, such as... Figures 1-5 As shown, it includes a storage tank 1, and a support bucket 301 connected to the storage tank 1 via a drive assembly is provided inside the storage tank 1. An installation block 4 is fixed to the inner wall of the support bucket 301, and a separation tank 401 connected to the installation block 4 via a locking assembly is provided inside the installation block 4.

[0026] The upper end of the separator 401 is provided with a connecting rod 502 connected by a threaded assembly. The lower end of the connecting rod 502 is fixed with a filter plate 503. The outer wall of the filter plate 503 is fixed with a cylinder 504. The inner wall of the separator 401 is fixed with a support ring 6. The upper end face of the support ring 6 is fixed with a rubber ring 601. The lower end of the separator 401 is provided with a first scraper 701 connected by a rotating assembly. The end of the first scraper 701 is fixed with a second scraper 702. The side of the separator 401 is fixed with a discharge pipe 8. The discharge pipe 8 is internally threaded with a bolt plug 803. One end of the bolt plug 803 is fixed with a handwheel block 804. The upper end of the handwheel block 804 is provided with a locking assembly for fixing.

[0027] In a preferred embodiment, the drive assembly includes a drive shaft 3 that runs through the bottom of the storage tank 1, a support bucket 301 that is fixedly connected to the output end of the drive shaft 3, a slip ring 302 that is fixed to the outer wall of the support bucket 301, and a positioning slide rail 303 that is fixed to the inner wall of the storage tank 1. The slip ring 302 and the positioning slide rail 303 are slidably connected. A cover 2 that is rotatably connected to the upper end of the storage tank 1 via a rotating shaft is provided. The operator pours the culture medium containing stem cells into the separation tank 401. The drive shaft 3 is driven by a motor, which drives the support bucket 301 to rotate along the positioning slide rail 303 inside the storage tank 1 via the slip ring 302, ensuring the stability of the storage mechanism during centrifugation. The cell fluid inside the separation tank 401 will be separated by centrifugal force, in which the outer shell of the stem cells and the internal particles will be separated from the cell fluid. The cover 2 is then rotated to open.

[0028] In a preferred embodiment, the locking assembly includes a limiting plate 402 fixedly connected to the outer wall of the separation tank 401. The separation tank 401 is slidably connected inside the mounting block 4. The limiting plate 402 is fitted to the upper end face of the mounting block 4. A set of locking screws is provided inside one side of the limiting plate 402. The separation tank 401 is locked inside the mounting block 4 through the limiting plate 402. Then, the locking screws inside the side of the limiting plate 402 are locked to the mounting block 4 to achieve the installation of the separation tank 401 and improve the stability during operation.

[0029] In a preferred embodiment, the threaded assembly includes a bolt block 5 internally threaded to the upper end of the separator 401, a handle 501 fixed to the upper end of the bolt block 5, a connecting rod 502 fixedly connected to the lower end of the bolt block 5, a cylindrical body 504 fixedly connected to the filter plate 503, and a filter plate 503 fixedly connected to the connecting rod 502. The bolt block 5 is installed and disassembled from the separator 401 by rotating the handle 501, facilitating equipment maintenance and cleaning. The filter plate 503 and the cylindrical body 504 are fitted against the surface of the rubber ring 601 on the upper end face of the support ring 6, improving the height of the filter plate 503 and the cylindrical body 504. 4. During installation, a certain level of support is provided to ensure the stability of the internal filter plate 503. A stable filter plate 503 can effectively prevent displacement or damage caused by vibration or pressure changes. Then, the rubber ring 601 ensures the sealing between the filter plate 503 and the separation tank 401, and the filtered cell liquid after centrifugation is filtered inside the filter plate 503 and the cylinder 504, reducing the overflow of cell liquid at the edge when it is removed. The reduction of overflow not only reduces the loss of cell liquid, but also avoids the risk of contamination caused by overflow, thereby improving the reliability and safety of the entire filtration and extraction process.

[0030] In a preferred embodiment, the rotating assembly includes a drive rod 7 that runs through the lower end of the separation tank 401. A first scraper 701 is fixedly connected to the output end of the drive rod 7. The drive rod 7 at the bottom of the separation tank 401 is driven by a motor, which in turn drives the first scraper 701 to rotate against the bottom surface of the separation tank 401. This effectively scrapes up cells or impurities deposited at the bottom, allowing them to re-enter the solution for centrifugation, thereby improving separation efficiency. Then, a second scraper 702 rotates against the inner wall of the separation tank 401, removing cells or impurities attached to the inner wall and ensuring the smooth progress of the centrifugation process, further improving separation efficiency. The mechanical scraping action can more thoroughly remove residues inside the separation tank 401, enhancing the cleaning effect. The scraper can cover the bottom and inner wall of the separation tank 401, ensuring thorough cleaning without dead angles and avoiding the impact of residues on subsequent experiments.

[0031] In a preferred embodiment, the locking assembly includes a locking block 805 fixed to the upper end of the handwheel block 804. A set of locking screws is disposed inside the locking block 805. A positioning ring 801 is fixed to the outer wall of the discharge pipe 8. Threaded holes 802 are evenly distributed on the outer wall of the positioning ring 801. The discharge pipe 8 is used to pour out cell fluid. When connecting the discharge pipe 8, the handwheel block 804 is threaded onto the bolt plug 803 inside the discharge pipe 8. Then, the locking screws inside the locking block 805 at the upper end of the handwheel block 804 are rotated. The bolt plug 803 is locked in place with the threaded hole 802 inside the positioning ring 801 on the outer wall of the discharge tube 8 to ensure the stability of the connection. The double locking mechanism significantly enhances the connection stability between the bolt plug 803 and the discharge tube 8, ensuring that the bolt plug 803 will not fall off due to vibration or centrifugal force during centrifugation. The stable connection of the bolt plug 803 can effectively prevent cell fluid from leaking from the discharge tube 8 during centrifugation. The solid connection of the bolt plug 803 avoids potential safety hazards caused by falling off during centrifugation.

[0032] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A filtration and extraction device for stem cell exosomes, characterized in that: Includes a storage tank (1), the storage tank (1) is provided with a support bucket (301) connected by a drive assembly inside the storage tank (1), the inner wall of the support bucket (301) is fixed with an installation block (4), the installation block (4) is provided with a separation tank (401) connected by a locking assembly inside the installation block (4); The upper end of the separator (401) is provided with a connecting rod (502) connected by a threaded assembly. The lower end of the connecting rod (502) is fixed with a filter plate (503). The outer wall of the filter plate (503) is fixed with a cylinder (504). The inner wall of the separator (401) is fixed with a support ring (6). The upper end face of the support ring (6) is fixed with a rubber ring (601). The lower end of the separator (401) is provided with a first scraper (701) connected by a rotating assembly. The end of the first scraper (701) is fixed with a second scraper (702). The side of the separator (401) is fixed with a discharge pipe (8). The discharge pipe (8) is internally threaded with a bolt plug (803). One end of the bolt plug (803) is fixed with a handwheel block (804). The upper end of the handwheel block (804) is provided with a locking assembly for fixing.

2. The stem cell exosome filtration and extraction device according to claim 1, characterized in that: The drive assembly includes a storage tank (1) with a drive shaft (3) running through the bottom. The support bucket (301) is fixedly connected to the output end of the drive shaft (3). A slip ring (302) is fixed to the outer wall of the support bucket (301). A positioning slide rail (303) is fixed to the inner wall of the storage tank (1). The slip ring (302) and the positioning slide rail (303) are slidably connected. A cover (2) is provided at the upper end of the storage tank (1) and is rotatably connected by a rotating shaft.

3. The stem cell exosome filtration and extraction device according to claim 1, characterized in that: The locking assembly includes a limiting plate (402) fixedly connected to the outer wall of the separation tank (401). The separation tank (401) is slidably connected inside the mounting block (4). The limiting plate (402) is fitted to the upper end face of the mounting block (4). A set of locking screws is provided inside one side of the limiting plate (402).

4. The stem cell exosome filtration and extraction device according to claim 1, characterized in that: The threaded assembly includes a bolt block (5) with an internal threaded connection at the upper end of the separator (401), a handle (501) fixed at the upper end of the bolt block (5), and a connecting rod (502) fixedly connected at the lower end of the bolt block (5).

5. The stem cell exosome filtration and extraction device according to claim 1, characterized in that: The rotating assembly includes a drive rod (7) that runs through the lower end of the separation tank (401), and the first scraper (701) is fixedly connected to the output end of the drive rod (7).

6. The stem cell exosome filtration and extraction device according to claim 1, characterized in that: The locking assembly includes a locking block (805) fixed to the upper end of the handwheel block (804), and a set of locking screws is provided inside the locking block (805).

7. The stem cell exosome filtration and extraction device according to claim 1, characterized in that: The outer wall of the discharge pipe (8) is fixed with a positioning ring (801), and the outer wall of the positioning ring (801) is uniformly provided with threaded holes (802).

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