Flow type pipe module with CCD (Charge Coupled Device) detection function

Abstract

The utility model discloses a flow type pipe module with a CCD detection function. The flow type pipe module mainly comprises a flow type pipe assembly, a CCD detection assembly and a transmission assembly. The flow type pipe assembly comprises a flow type pipe rotating disc and a plurality of flow type pipe frames arranged on the flow type pipe rotating disc. The transmission assembly is installed below the flow type pipe rotating disc and used for driving the flow type pipe rotating disc to rotate. The transmission assembly is installed on the substrate, and the CCD detection assembly is installed above the substrate through a supporting column. The CCD detection assembly is used for scanning and identifying all the flow type pipes located on the flow type pipe frame in the rotating process of the flow type pipe rotating disc. According to the flow type pipe module with the CCD detection function, the types and the accurate positions of flow type pipes randomly placed on the flow type pipe frame can be automatically distinguished through an image recognition algorithm.

Description

Technical Field

[0001] This utility model relates to the field of medical testing equipment technology, and more specifically to a flow cytometer module with CCD detection function. Background Technology

[0002] Flow cytometer tubes, also known as flow cytometer tubes or flow cell tubes, are used in flow cytometry. A flow cytometer is a tool for cell analysis that performs statistical analysis of cells based on the intensity of light signals generated by cells of different sizes, shapes, and surface fluorescent markers. With technological advancements, scientists have placed higher demands on the number of fluorescence detections and the morphological data of cells in flow cytometry. This requires acquiring image information of samples while performing flow cytometry analysis, and accurately identifying flow cytometer tubes in different states, posing a significant challenge to current technologies. Utility Model Content

[0003] To address the shortcomings of existing technologies, the purpose of this utility model is to provide a flow cytometer module with CCD detection function, which can automatically distinguish the type and accurate position of randomly placed flow cytometers on the flow cytometer rack through image recognition algorithms.

[0004] The technical solution adopted by this utility model to solve the technical problem is: a flow cytometer module with CCD detection function, comprising a flow cytometer module mainly including a flow cytometer assembly, a CCD detection assembly, and a transmission assembly; the flow cytometer assembly includes a flow cytometer turntable and a plurality of flow cytometer supports disposed on the flow cytometer turntable; the transmission assembly is installed below the flow cytometer turntable and is used to drive the flow cytometer turntable to rotate; the transmission assembly is installed on a substrate, and the CCD detection assembly is installed above the substrate through a support column; the CCD detection assembly is used to scan and identify each flow cytometer located on the flow cytometer supports during the rotation of the flow cytometer turntable.

[0005] Furthermore, the flow meter rotary table is provided with at least two flow meter support zones, and each flow meter support zone may or may not be equipped with a flow meter support as needed.

[0006] Furthermore, a sector partition cover is provided at the center of the flow tube turntable, and the sector partition cover is marked with several sectors; each sector corresponds one-to-one with a section of the flow tube rack.

[0007] Furthermore, positioning protrusions are provided between each of the aforementioned flow tube rack sections, and positioning grooves matching the positioning protrusions are provided on both sides of each of the aforementioned flow tube racks.

[0008] Furthermore, each of the aforementioned flow tube supports is equipped with a handle.

[0009] Furthermore, the CCD detection component includes a debugging housing, a CCD camera disposed on the top of the debugging housing, and an optical component disposed inside the debugging housing.

[0010] Furthermore, the optical components include a fill light, a diffuser, and a reflective lens; the reflective lens is fixed by a lens mounting base, the fill light is fixed by a fill light mounting base, and the diffuser is tilted below the fill light.

[0011] The beneficial effects of this invention are as follows: Compared with the prior art, this invention provides a flow cytometer module with CCD detection function. It distinguishes different flow cytometer rack sections by sector-based partitioning. Users can randomly place flow cytometers at any position on any sector of the flow cytometer rack. A transmission component drives the flow cytometer turntable to complete a 360° rotation. After the CCD detection component scans the flow cytometers on the turntable, the acquired image data is transmitted to a PC via network cable for relevant calculations. An image recognition algorithm automatically distinguishes the type and precise position of the randomly placed flow cytometers on the rack. This invention achieves the design goal of fully automatic flow cytometer identification with a low-cost solution, eliminating the need for manual differentiation of different flow cytometers before experiments, thus significantly saving manpower. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the flow tube module provided by this utility model.

[0013] Figure 2 This is a schematic diagram of the CCD detection component in the flow tube module provided by this utility model.

[0014] The components are as follows: 1-CCD detection component; 2-support column; 3-transmission component; 4-substrate; 5-positioning groove; 6-positioning protrusion; 7-flow tube turntable; 8-flow tube rack; 9-flow tube; 10-handle; 11-sector partition cover; 12-module support base; 13-CCD camera; 14-lens mounting base; 15-adjustment housing; 16-reflective lens; 17-fill light mounting base; 18-diffuser; 19-fill light. Detailed Implementation

[0015] To facilitate understanding of this utility model, a more comprehensive description will be given below with reference to the accompanying drawings. The drawings illustrate preferred embodiments of this utility model. However, this utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of this utility model.

[0016] Example

[0017] like Figure 1 As shown, a flow cytometer module with CCD detection function includes a flow cytometer module, mainly comprising a flow cytometer assembly, a CCD detection assembly 1, and a transmission assembly 3. The flow cytometer assembly includes a flow cytometer turntable 7 and several flow cytometer supports 8 disposed on the flow cytometer turntable 7. The transmission assembly 3 is installed below the flow cytometer turntable 7 (the flow cytometer turntable 7 in the figure is transparent for clearer representation of the structure of the transmission assembly 3) and is used to drive the flow cytometer turntable 7 to rotate. The transmission assembly 3 is mounted on a base plate 4, and the CCD detection assembly 1 is mounted above the base plate 4 via a support column 2. The base plate 4 is mounted on the instrument (not shown in this embodiment) via a module support base 12. The CCD detection assembly 1 is used to scan and identify each flow cytometer 9 located on the flow cytometer supports 8 during the rotation of the flow cytometer turntable 7.

[0018] The flow cytometry turntable 7 has at least two flow cytometry support zones, and each flow cytometry support zone can be equipped with or without a flow cytometry support 8 as needed. A sector dividing cover 11 is located at the center of the flow cytometry turntable 7, and the sector dividing cover 11 is marked with several sectors; each sector corresponds one-to-one with a flow cytometry support zone. In this embodiment, there are four flow cytometry support zones, and the sector dividing cover 11 is marked with four sectors, each sector corresponding to one flow cytometry support zone. The sector dividing cover 11 is marked with Arabic numerals 1, 2, 3, and 4. The user randomly places the flow cytometry tube 9 at any position on the flow cytometry support 8 in any sector, and rotates the flow cytometry turntable 7 through the transmission component 3 to complete a full 360° rotation. After the CCD detection component 1 scans all the flow cytometry tubes 9 on the flow cytometry turntable 7, the type of flow cytometry tube 9 placed by the user will be automatically displayed on the software interface. In this embodiment, the transmission component 3 consists of a stepper motor, a synchronous belt, a synchronous pulley, and a code disk. The stepper motor, synchronous belt, and synchronous pulley are used to transmit power, and the code disk is used to maintain rotational accuracy.

[0019] Each of the aforementioned flow tube rack sections is provided with a positioning protrusion 6, and each of the aforementioned flow tube racks 8 has a positioning groove 5 on both sides that matches the positioning protrusion 6. When placing the flow tube rack 8, the positioning groove 5 and the positioning protrusion 6 are engaged and positioned to prevent the flow tube rack 8 placed on the flow tube turntable 7 from moving relative to each other due to the rotation of the flow tube turntable 7.

[0020] Each of the aforementioned flow tube racks 8 is equipped with a handle 10. The handle 10 is designed to facilitate the taking and placing of the flow tube racks 8.

[0021] like Figure 2 As shown, to more clearly illustrate the internal structure of the CCD detection component 1, the attached diagram... Figure 2The debugging housing 15 is transparent. The CCD detection component 1 includes the debugging housing 15, a CCD camera 13 located on the top of the debugging housing 15, and an optical component located inside the debugging housing 15. The optical component includes a fill light 19, a diffuser 18, and a reflective lens 16; the reflective lens 16 is fixed by a lens mounting base 14, the fill light 19 is fixed by a fill light mounting base 17, and the diffuser 18 is obliquely disposed below the fill light 19. In this embodiment, there are four reflective lenses 16, divided into two groups, which are obliquely disposed on both sides inside the debugging housing 15. The fill light 19 has a U-shaped structure and is fixed below the fill light mounting base 17. There are two diffusers 18, and the two diffusers 18 are tilted in opposite directions.

[0022] The light from the supplementary light 19 is diffused into a uniform white soft light after passing through the diffuser 18, illuminating the flow cytometry tube array to be photographed on the flow cytometry tube rack 8. The illuminated flow cytometry tubes 9 form an image through the reflector 16 and are transmitted to the CCD camera 13. The image captured by the CCD camera 13 is transmitted to the PC via a network cable for relevant processing. Through image recognition algorithms, the instrument can automatically distinguish the type and precise position of the randomly placed flow cytometry tubes 9 on the flow cytometry tube rack 8 (empty flow cytometry tubes, absolute counting tubes, flow cytometry tubes with caps, and holes without flow cytometry tubes).

[0023] The above embodiments are only used to illustrate the present utility model and are not intended to limit the present utility model. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present utility model. Therefore, all equivalent technical solutions also fall within the scope of the present utility model. The patent protection scope of the present utility model should be defined by the claims.

Claims

1. A flow cytometer module with CCD detection function, characterized in that: The flow cytometry module mainly includes a flow cytometry tube assembly, a CCD detection assembly, and a transmission assembly. The flow cytometry tube assembly includes a flow cytometry tube turntable and several flow cytometry tube supports mounted on the turntable. The transmission assembly is installed below the turntable and is used to drive the turntable to rotate. The transmission assembly is mounted on a substrate, and the CCD detection assembly is mounted above the substrate via support columns. The CCD detection assembly is used to scan and identify each flow cytometry tube located on the flow cytometry tube supports during the rotation of the turntable.

2. A flow cytometer module with CCD detection function as described in claim 1, characterized in that: The flow meter turntable is provided with at least two flow meter support zones, and each flow meter support zone may or may not be equipped with a flow meter support as needed.

3. A flow cytometer module with CCD detection function as described in claim 2, characterized in that: The flow tube turntable has a sector dividing cover at its center, and the sector dividing cover is marked with several sectors; each sector corresponds one-to-one with a section of the flow tube rack.

4. A flow cytometer module with CCD detection function as described in claim 2, characterized in that: Each of the aforementioned flow rack sections is provided with a positioning protrusion, and each of the aforementioned flow racks is provided with a positioning groove on both sides that matches the positioning protrusion.

5. A flow cytometer module with CCD detection function as described in claim 1, characterized in that: Each of the aforementioned flow tube supports is equipped with a handle.

6. A flow cytometer module with CCD detection function as described in claim 1, characterized in that: The CCD detection component includes a debugging housing, a CCD camera located on the top of the debugging housing, and optical components located inside the debugging housing.

7. A flow cytometer module with CCD detection function as described in claim 6, characterized in that: The optical components include a fill light, a diffuser, and a reflective lens; the reflective lens is fixed by a lens mounting base, the fill light is fixed by a fill light mounting base, and the diffuser is tilted below the fill light.

Images