Virus detection device

Abstract

The utility model discloses a virus detection device and belongs to the technical field of kits. The virus detection device comprises a first shell, a first connecting rod and a second connecting rod are rotationally mounted on the side wall of the first shell, and the first connecting rod and the second connecting rod are arranged in parallel; the first connecting rod is provided with a test paper roll, the test paper roll comprises a plurality of test paper strips, the plurality of test paper strips are sequentially arranged on the surface of the test paper roll, the tail end of the test paper roll is wound on the periphery of the first connecting rod, the starting end of the test paper roll is wound on the periphery of the second connecting rod, and the test paper strips are used for detecting samples. The first connecting rod or the second connecting rod is rotated to enable the starting end of the test paper roll to rotate around the second connecting rod, so that the plurality of test paper strips are driven to move. According to the virus detection device, a new test strip is moved to a sample adding position through rotation of the first connecting rod or the second connecting rod, so that a plurality of samples are detected.

Description

Technical Field

[0001] This invention belongs to the field of reagent kits, and particularly relates to a virus detection device. Background Technology

[0002] Ebola virus (EBOV) is a negative-sense RNA filovirus with six reported subtypes: Zaire, Bundibugyo, Sudan, Tai Forest, Reston, and Bumbari. Ebola virus can be transmitted through direct contact with infectious blood, bodily fluids, and excrement between humans, between humans and animals, and between animals, exhibiting diverse transmission routes. Since its initial discovery in 1976, there have been more than 20 outbreaks. Infected patients initially present with flu-like symptoms such as fever, abdominal pain, diarrhea, and vomiting, later developing subcutaneous hemorrhage, multiple organ failure, and shock. The overall mortality rate is as high as 40%–50%. This high mortality rate is due to the virus's rapid attack on the human body once it enters, exceeding the speed of modern medical treatment. Currently, there are no effective treatments or preventative measures in the medical field. Therefore, failure to detect and effectively control the virus in a timely manner can easily lead to large-scale outbreaks, posing a significant threat to global public health and human health.

[0003] Most Ebola virus test kits currently available on the market use ELISA and fluorescent PCR methods. These two methods involve many steps, take a long time to produce results, and have a limited number of samples that can be tested, which can cause public health agencies to miss the best opportunity for infection control.

[0004] Therefore, developing an efficient, rapid, and convenient Ebola virus detection kit capable of quickly detecting multiple samples is of great significance for the prevention and control of Ebola virus transmission. Summary of the Invention

[0005] This invention provides a virus detection device that enables the detection of multiple samples using a single device, and allows for the insertion of new reagent rolls after the existing rolls are used up, thereby improving detection efficiency.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] This utility model discloses a virus detection device, including a first housing, a first connecting rod and a second connecting rod rotatably mounted on the side wall of the first housing, and the first connecting rod and the second connecting rod are arranged parallel to each other.

[0008] A test strip roll is provided on the first connecting rod. The test strip roll includes multiple test strips, which are sequentially arranged on the surface of the test strip roll. The end of the test strip roll is wrapped around the outer periphery of the first connecting rod, and the starting end of the test strip roll is wrapped around the outer periphery of the second connecting rod. The test strips are used to detect samples. Rotating the first or second connecting rod causes the starting end of the test strip roll to rotate around the second connecting rod, thereby moving the multiple test strips.

[0009] In another embodiment of this utility model, a first knob is provided at the end of the first connecting rod, and rotating the first knob causes the first connecting rod to rotate.

[0010] Furthermore, a first protrusion is mounted on the surface of the first knob, and a first groove is provided on the side wall of the first housing. The first protrusion can be engaged into the first groove to lock the first connecting rod.

[0011] In another embodiment of this utility model, a second knob is provided at the end of the second connecting rod, and rotating the second knob causes the second connecting rod to rotate.

[0012] Furthermore, a second protrusion is mounted on the surface of the second knob, and a second groove is provided on the side wall of the first housing. The second protrusion can be engaged into the second groove to lock the second connecting rod.

[0013] In another embodiment of this utility model, the test strip roll further includes a back plate, and multiple test strips are sequentially arranged on the surface of the back plate. The end of the back plate is wrapped around the outer periphery of the first connecting rod, and the starting end of the back plate is wrapped around the outer periphery of the second connecting rod. Rotating the first connecting rod or the second connecting rod causes the starting end of the back plate to rotate around the second connecting rod, thereby driving the multiple test strips to move.

[0014] In another embodiment of this utility model, the test strip includes a sample pad, a conjugate pad, and a detection pad. One end of the sample pad is overlapped with the front end of the conjugate point, and the rear end of the conjugate pad is overlapped with one end of the detection pad. The sample pad is used to receive the sample to be tested. The conjugate pad is coated with an antibody labeled against Ebola virus. The detection pad is provided with a control line and a detection line. The control line is coated with a secondary antibody, and the detection line is coated with an antibody against Ebola virus.

[0015] Furthermore, the quality control line is provided with multiple lines, and the detection line is provided with multiple lines.

[0016] Furthermore, the test strip also includes an absorbent pad, with the other end of the detection pad overlapping the absorbent pad.

[0017] In another embodiment of the present invention, a second housing is also included. A slot is installed on the outer surface of the first housing, and a protrusion is installed on the bottom of the side wall of the second housing. The protrusion is embedded in the slot, so that the first housing and the second housing are nested together.

[0018] Furthermore, the second shell surface is provided with a sample dispensing port and a reaction window, the sample dispensing port being used for sample dispensing and the reaction window being used for observing the detection results.

[0019] The advantages of this utility model compared with the prior art are:

[0020] The virus detection device disclosed in this utility model moves and unfolds the test strip roll wound on the first or second link by rotating the first or second link, thereby moving multiple test strips on the test strip roll to the sample application position for sample application. After observing and recording the test results, the first or second link is rotated again to move a new test strip to the sample application position, thus realizing the detection of multiple samples by one detection device. Attached Figure Description

[0021] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 A schematic diagram of the virus detection device provided in this embodiment of the utility model;

[0023] Figure 2 A schematic diagram of the knob and groove structure provided in the embodiment of this utility model;

[0024] Figure 3 This is a schematic diagram of the test paper roll structure provided in an embodiment of the present utility model;

[0025] Figure 4 Schematic diagrams of different combinations of quality control lines and detection lines provided in embodiments of this utility model;

[0026] Figure 5 A schematic diagram of the first and second housing structures provided in this embodiment of the utility model;

[0027] The labels for the attached figures are as follows:

[0028] 1. Backplate; 2. Absorbent pad; 3. Detection pad; 4. Binding pad; 5. Sample pad; 6. Quality control line; 7. Detection line; 8. First housing; 9. Second housing; 100. First connecting rod; 101. Second connecting rod; 11. Test paper roll; 110. Test paper strip; 120. First knob; 121. Second knob; 13. Slot; 140. First groove; 141. Second groove; 15. Reaction window; 16. Sample dispensing port; 17. Raised bar; 180. First protrusion; 181. Second protrusion. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] like Figures 1-5 As shown, this utility model discloses a virus detection device, including a first housing 8, a first connecting rod 100, a second connecting rod 101, a test strip roll 11, and test strips 110. The first connecting rod 100 and the second connecting rod 101 are installed through the side wall of the first housing 8, and the first connecting rod 100 and the second connecting rod 101 are arranged parallel to each other. A test strip roll 11 is disposed on the first connecting rod 100 and sleeved on the first connecting rod 100. Multiple test strips 110 are sequentially disposed on the surface of the test strip roll 11, and the multiple test strips 110 are spliced ​​together. The end of the test strip roll 11 is wrapped around the outer periphery of the first connecting rod 100, and the beginning end of the test strip roll 11 is wrapped around the outer periphery of the second connecting rod 101. In other words, the test strip roll 11 connects the first connecting rod 100 and the second connecting rod 101. Each test strip 110 is equipped with a test reagent to detect the sample. Rotating the first connecting rod 100 or the second connecting rod 101 causes the starting end of the test strip roll 11 to rotate around the second connecting rod 101, thereby driving multiple test strips 110 to move and realize the replacement of multiple test strips 110.

[0031] The virus detection device disclosed in this utility model rotates the first connecting rod 100 or the second connecting rod 101 to move and flatten the test strip roll 11 wound on the first connecting rod 100 and the second connecting rod 101, thereby moving multiple test strips 110 on the test strip roll 11 to the sample application position for sample application. After observing and recording the test results, the first connecting rod 100 or the second connecting rod 101 is rotated again to move a new test strip 110 to the sample application position for sample application, thus realizing the detection of multiple samples by one detection device.

[0032] It should be noted that the test strip roll 11 in the detection device can realize the function of one card for multiple tests. When the test strip roll 11 is used up, an unused test strip roll 11 can be reloaded to realize the function of reusing the shell.

[0033] To facilitate rotation of the first connecting rod 100, a cylindrical first knob 120 is provided at the end of the first connecting rod 100. Rotating the first knob 120 causes the first connecting rod 100 to rotate. Understandably, for ease of gripping, the first knob 120 is relatively large and is located on the outside of the first housing 8. Furthermore, the first knob 120 can have various shapes, such as rectangular, elliptical, or octagonal. Additionally, the position of the first knob 120 can also vary; for example, it can be located at one end of the first connecting rod 100, or at both ends of the first connecting rod 100. Further details are omitted here.

[0034] During the replacement of test strip 110, it is sometimes necessary to fix the first connecting rod 100, such as... Figure 2 As shown, a first protrusion 180 is mounted on the surface of the first knob 120, and a first groove 140 is provided on the side wall of the first housing 8. The first protrusion 180 can be engaged with the first groove 140 to lock the first connecting rod 100. It is understood that the shape of the first protrusion 180 is one of a sphere, a cuboid, or a cube, and the shape of the first groove 140 is one of a sphere, a cuboid, or a square. When it is necessary to rotate the first connecting rod 100 again to use a new test strip 110, simply pull the first knob 120 away from the first housing 8, causing the first protrusion 180 to disengage from the first groove 140, thus easily releasing the locking of the first connecting rod 100. The operation is simple and quick.

[0035] It should be noted that when the first knob 120 is pulled away from the first housing 8, the first protrusion 180 moves away from the first groove 140. Rotating the first knob 120 will cause the first connecting rod 100 to rotate, which in turn will cause the test strip roll 11 to unfold. When the sample pad 5 on the test strip 110 appears below the sample application port 16, the first knob 120 can be pushed closer to the first housing 8, causing the first protrusion 180 to engage with the first groove 140. At this point, the first knob 120 will be fixed in place, and the first connecting rod 100 will not be able to rotate, thus preventing the test strip 110 from moving.

[0036] To facilitate rotation of the second connecting rod 101, a cylindrical second knob 121 is provided at the end of the second connecting rod 101. Rotating the second knob 121 causes the second connecting rod 101 to rotate. Understandably, for ease of gripping, the second knob 121 is relatively large and located on the outside of the first housing 8. Furthermore, the second knob 121 can have various shapes, such as rectangular, elliptical, or octagonal. Additionally, the position of the second knob 121 can also vary; for example, it can be located at one end of the second connecting rod 101, or at both ends of the second connecting rod 101. Further details are omitted here.

[0037] During the replacement of test strip 110, it is sometimes necessary to fix the second connecting rod 101, such as... Figure 2 As shown, a second protrusion 181 is mounted on the surface of the second knob 121, and a second groove 141 is provided on the side wall of the first housing 8. The second protrusion 181 can be engaged with the second groove 141 to lock the second connecting rod 101. It is understood that the shape of the second protrusion 181 is one of a sphere, a cuboid, or a cube, and the shape of the second groove 141 is one of a sphere, a cuboid, or a square. When it is necessary to rotate the second connecting rod 101 again to use a new test strip 110, simply pull the second knob 121 away from the first housing 8, causing the second protrusion 181 to disengage from the second groove 141, easily releasing the lock on the second connecting rod 101. The operation is simple and quick.

[0038] It should be noted that when the second knob 121 is pulled away from the first housing 8, the second protrusion 181 disengages from the second groove 141. Rotating the second knob 121 will cause the second connecting rod 101 to rotate, which in turn will cause the test strip roll 11 to unfold. When the sample pad 5 on the test strip 110 appears below the sample application port 16, the second knob 121 can be pushed closer to the first housing 8, causing the second protrusion 181 to engage with the second groove 141. At this point, the second knob 121 will be fixed in place, and the second connecting rod 101 will not be able to rotate, thus preventing the test strip 110 from moving.

[0039] like Figure 3 As shown, the test strip roll 11 also includes a back plate 1, and multiple test strips 110 are sequentially arranged on the surface of the back plate 1. The end of the back plate 1 is wrapped around the outer periphery of the first connecting rod 100, and the starting end of the back plate 1 is wrapped around the outer periphery of the second connecting rod 101. Rotating the first connecting rod 100 or the second connecting rod 101 causes the starting end of the back plate 1 to rotate around the second connecting rod 101, thereby driving the multiple test strips 110 to move. The two ends of the back plate 1 are respectively wrapped around the first connecting rod 100 and the second connecting rod 101. When rotating the connecting rod, the back plate 1 can move smoothly, reducing the shaking and collision of the test strips 110 and ensuring the stable operation of the equipment.

[0040] The structure of test strip 110 is as follows: Figure 3 As shown, the test strip 110 includes a sample pad 5, a conjugate pad 4, and a detection pad 3, which are overlapped end-to-end. One end of the sample pad 5 overlaps with the front end of the conjugate pad, and the rear end of the conjugate pad 4 overlaps with one end of the detection pad 3. The sample pad 5 is used to receive the sample to be tested and to uniformly diffuse the sample. The conjugate pad 4 is coated with an antibody labeled against Ebola virus. The detection pad 3 has a control line 6 and a detection line 7. The control line 6 is coated with a secondary antibody, and the detection line 7 is coated with an antibody against Ebola virus. As the starting point for sample detection, the sample pad 5 is responsible for uniformly receiving and diffusing the sample to be tested, ensuring that the sample can uniformly contact the subsequent conjugate pad 4 and detection pad 3. The conjugate pad 4 is coated with an antibody labeled against Ebola virus, which can specifically bind to the Ebola virus antigen in the sample to form an antigen-antibody complex, providing a basis for subsequent detection. The material coated on the conjugate pad 4 includes colloidal gold or quantum dots. The control line 6 on the test pad 3 is coated with a secondary antibody to verify whether the detection device is working properly. The test line 7 on the test pad 3 is coated with an anti-Ebola virus antibody for specific detection of Ebola virus antigen in the sample. The test result is determined by observing the color development of the test line 7 and the control line 6: if both the test line 7 and the control line 6 change color, Ebola virus antigen is present in the sample; if only the control line 6 changes color, Ebola virus antigen is not present in the sample; and if the control line 6 does not change color, the test is invalid. The detection principle of the detection device disclosed in this utility model is one of the following: double antibody sandwich method for antigen detection, competitive method for antigen detection, or small molecule sandwich method for antigen detection.

[0041] The detection pad 3 is a strip-shaped fiber chromatography material, which can be any one of nitrocellulose membrane, nylon membrane, polyvinylidene fluoride membrane, polyamide membrane, or DEAE cellulose membrane. The material sprayed onto the binding pad 4 can be any one of fluorescent particles, enzymes, chemiluminescent substances, nanomaterials (carbon nanoparticles, non-metallic nanoparticles, metallic nanoparticles, latex microspheres, quantum dots), magnetic microparticles, or rare earth ions. The fluorescent particles include, but are not limited to, anthocyanin series fluorescent dyes, carboxyfluorescein, fluorescein isothiocyanate, 2-methoxyfluorescein, rhodamine, phycoerythrin, and lanthanide chelates. The enzymes include, but are not limited to, horseradish peroxidase, alkaline phosphatase, and luciferase. The chemiluminescent substances include, but are not limited to, acridinium ester, isoluminol, and ruthenium tripyridine. The carbon nanoparticles include, but are not limited to, carbon nanotubes, carbon nanofibers, and carbon nanospheres. The non-metallic nanoparticles include, but are not limited to, colloidal selenium. The metallic nanoparticles include, but are not limited to, nano-gold particles, nano-silver particles, nano-nickel particles, and nano-palladium particles. The latex microspheres are polystyrene latex microspheres modified with chemical groups, including but not limited to carboxyl, amino, hydroxyl, thiol, and sulfate groups. The quantum dots include, but are not limited to, elemental semiconductor quantum dots, compound semiconductor quantum dots, alloy quantum dots, core-shell structure quantum dots, and doped quantum dots. The magnetic particles include, but are not limited to, magnetic metals / magnetic metal oxides (such as Fe, Co, Ni and their corresponding oxides and alloys) and their composites formed with organic or inorganic materials through surface modification. The rare earth ions include, but are not limited to, scandium ions, yttrium ions, and cerium ions.

[0042] Furthermore, the test strip 110 also includes an absorbent pad 2, with the other end of the detection pad 3 overlapping the absorbent pad 2. The absorbent pad 2 is used to assist the sample in flowing on the test strip 110 and control the sample flow rate, preventing the sample from flowing too fast or too slow, and ensuring that the sample stays in the detection area for a sufficient time to react fully.

[0043] like Figure 4 As shown, the number of control lines 6 and test lines 7 can vary. Multiple control lines 6 and multiple test lines 7 can be configured to meet different testing scenarios and needs. Specifically, one test line 7 and one control line 6 can be sprayed onto the test pad 3 of a test strip 110, with one test line 7 detecting Ebola virus. Alternatively, two test lines 7 and two control lines 6 can be sprayed onto the test pad 3 of a test strip 110, with both test lines 7 used to detect Ebola virus. (See appendix.) Figure 4 One test strip 110 has a test pad 3 coated with one test line 7 and two control lines 6. The test line 7 can detect Ebola virus. Alternatively, one test strip 110 has a test pad 3 coated with two test lines 7 and one control line 6. Both test lines 7 are used to detect Ebola virus.

[0044] To protect the internal test strip 110 and other components, such as Figure 5 As shown, the detection device also includes a second housing 9. A slot 13 is installed on the outer surface of the first housing 8, and a protrusion 17 is installed on the bottom of the side wall of the second housing 9. The protrusion 17 is embedded in the slot 13, so that the first housing 8 and the second housing 9 are nested together. The first housing 8 and the second housing 9 are tightly integrated, making the whole device more compact and lightweight. At the same time, the design of the slot 13 and the protrusion 17 makes it easy to disassemble and reassemble the first housing 8 and the second housing 9.

[0045] Furthermore, the surface of the second housing 9 is provided with a sample dispensing port 16 and a reaction window 15. The sample dispensing port 16 is used for sample dispensing, and the reaction window 15 is used for observing the test results. The sample dispensing port 16 and the reaction window 15 on the surface of the second housing 9 allow users to complete sample dispensing and result observation on an integrated platform. The sample dispensing port 16 ensures that the sample is accurately dispensed onto the sample pad 5 of the test strip 110. It should be noted that the shape of the sample dispensing port 16 is one of the following: ellipse, circle, rectangle, and square, or any combination of two shapes; the shape of the reaction window 15 is one of the following: ellipse, circle, rectangle, and square, or any combination of two shapes.

[0046] The detection device disclosed in this utility model only requires rotating a knob to use one reagent device to test multiple samples. After the test strip 11 is used up, a new test strip 11 can be loaded for continued use, and results are produced quickly, which greatly improves detection efficiency and is of great significance for preventing and controlling the infection and spread of Ebola virus.

[0047] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this utility model, and these modifications or substitutions should all be covered within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.

Claims

1. A virus detection device, characterized in that: The system includes a first housing, on which a first connecting rod and a second connecting rod are rotatably mounted, with the first connecting rod and the second connecting rod arranged parallel to each other. A test strip roll is provided on the first connecting rod. The test strip roll includes multiple test strips, which are sequentially arranged on the surface of the test strip roll. The end of the test strip roll is wrapped around the outer periphery of the first connecting rod, and the starting end of the test strip roll is wrapped around the outer periphery of the second connecting rod. The test strips are used to detect samples. Rotating the first or second connecting rod causes the starting end of the test strip roll to rotate around the second connecting rod, thereby moving the multiple test strips.

2. The virus detection device according to claim 1, characterized in that, The first link is provided with a first knob at its end, and rotating the first knob causes the first link to rotate.

3. The virus detection device according to claim 2, characterized in that, The surface of the first knob is provided with a first protrusion, and the side wall of the first housing is provided with a first groove. The first protrusion can be engaged into the first groove to lock the first connecting rod.

4. The virus detection device according to claim 1, characterized in that, The second link is provided with a second knob at its end. Rotating the second knob causes the second link to rotate.

5. The virus detection device according to claim 4, characterized in that, The surface of the second knob is provided with a second protrusion, and the side wall of the first housing is provided with a second groove. The second protrusion can be engaged into the second groove to lock the second connecting rod.

6. The virus detection device according to claim 1, characterized in that, The test strip roll also includes a back plate, and multiple test strips are sequentially arranged on the surface of the back plate. The end of the back plate is wrapped around the outer periphery of the first connecting rod, and the starting end of the back plate is wrapped around the outer periphery of the second connecting rod. Rotating the first connecting rod or the second connecting rod causes the starting end of the back plate to rotate around the second connecting rod, thereby driving the multiple test strips to move.

7. The virus detection device according to claim 1, characterized in that, The test strip includes a sample pad, a conjugate pad, and a detection pad. One end of the sample pad is overlapped with the front end of the conjugate pad, and the rear end of the conjugate pad is overlapped with one end of the detection pad. The sample pad is used to receive the sample to be tested. The conjugate pad is coated with an antibody labeled against Ebola virus. The detection pad is provided with a control line and a detection line. The control line is coated with a secondary antibody, and the detection line is coated with an antibody against Ebola virus.

8. The virus detection device according to claim 7, characterized in that, The quality control line has multiple lines, and the testing line has multiple lines.

9. The virus detection device according to claim 7, characterized in that, The test strip also includes an absorbent pad, and the other end of the test pad is pressed against the absorbent pad.

10. The virus detection device according to claim 1, characterized in that, It also includes a second housing, wherein a slot is installed on the outer surface of the first housing, and a protrusion is installed on the bottom of the side wall of the second housing, the protrusion being embedded in the slot, so that the first housing and the second housing are nested and connected.

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