Quantitative liquid split charging system and split charging equipment thereof

Abstract

The utility model provides a liquid quantitative split charging system and split charging equipment, a split charging structure comprises a sample container, a plurality of quantitative containers, a split charging container, a switch valve and a power mechanism used for driving liquid in the sample container to flow into the quantitative containers, and the bottom of the sample container is connected with a main conveying pipeline. The main conveying pipeline is provided with a plurality of horizontal section branch pipelines, the horizontal section branch pipelines are connected with the bottoms of the corresponding quantitative containers through first vertical pipelines, and the tops of the quantitative containers are provided with second vertical pipelines communicated with the atmosphere. And a third vertical pipeline connected with a split charging container is also arranged on the horizontal section branch pipeline corresponding to the first vertical pipeline. The split charging equipment is used for being matched with a split charging structure for use and comprises a main body support and a pipe clamp used for fixing a corresponding pipeline. According to the utility model, the sub-packaging precision can be greatly improved, the sub-packaging power and the sub-packaging efficiency can be controlled as required, the sub-packaging operation is simple, and the operation efficiency is high.

Description

TECHNICAL FIELD

[0001] The utility model relates to liquid subpackaging technical field, especially a kind of liquid quantitative subpackaging system and subpackaging equipment. BACKGROUND

[0002] Cell therapy as a new treatment mode has developed rapidly in recent years, which mainly uses or modifies human body's own cells (such as stem cells, immune cells, etc.) to treat diseases. These cells can come from patients or others, or be produced by genetic engineering technology. At present, the cell therapy field mainly uses autologous cell therapy, but with the increase of treatment demand, the development of universal cell therapy has also become a trend. Universal cell therapy aims to make cell therapy products widely applicable to different patients through genetic modification, without considering the immune rejection between individuals. Compared with the preparation of autologous cell products, the production and preparation of universal cell therapy products have higher requirements in efficiency and throughput. The cell preparation subpackaging equipment on the market uses peristaltic pumps to subpackage the subpackaging bags one by one, which is not efficient, and due to factors such as peristaltic pump rubber tube wear and tear, it cannot meet the high-efficiency subpackaging demand of large throughput and multiple liquid bags.

[0003] The patent document with publication number CN218198915U discloses a medicine subpackager for western medicine research. Although it uses a communicating vessel to achieve the subpackaging function of medicine, it still has some shortcomings:

[0004] (1) In actual operation, the liquid level in each medicine subpackaging box cannot be absolutely level due to factors such as the levelness of the environment ground and the levelness of the medicine subpackaging box. After the medicine liquid is distributed to each medicine subpackaging box through the communicating vessel, the height difference (Δh) of the liquid in each medicine subpackaging box will cause a subpackaging precision error ΔV, ΔV = Δh × S, S is the cross-sectional area of the medicine subpackaging box. Similar to existing subpackaging equipment, the subpackaging precision error of the patent product is more than 2%.

[0005] (2) The patent cannot control the size of the subpackaging power, nor can it control the subpackaging efficiency.

[0006] (3) The communicating vessel is provided with a plurality of fourth valves, and when it is necessary to open or close the fourth valves, it is necessary to operate the opening and closing one by one, which is low in operation efficiency, and thus greatly reduces the subpackaging efficiency.

[0007] In addition, the existing quantitative dispensing method has low efficiency, such as the patent documents with publication numbers CN113022911A, CN118665799A, CN118665798A, etc. disclose a liquid dispensing method and equipment, but when implemented, they only simulate manual operation to realize automatic operation, and do not essentially solve the problem of rapid and accurate dispensing in a short time, especially the high-efficiency dispensing of large-volume cell liquid. Utility model content

[0008] In order to overcome the deficiencies of the prior art, the utility model provides a liquid quantitative dispensing system and dispensing equipment, which can greatly improve the dispensing precision, control the dispensing power and dispensing efficiency as needed, and has simple dispensing operation and high operation efficiency, thereby solving the problem of rapid and accurate liquid dispensing in a short time.

[0009] In order to at least achieve one of the above-mentioned purposes, the utility model adopts the technical scheme of:

[0010] The utility model discloses a liquid quantitative dispensing system first, comprising a sample container, a plurality of quantitative containers, a dispensing container, a switch valve and a power mechanism for driving the liquid in the sample container to flow into the quantitative container, the bottom of the sample container is connected with a total conveying pipeline, the total conveying pipeline has a plurality of horizontal branch pipeline, the horizontal branch pipeline is connected with the bottom of the corresponding quantitative container through the first vertical pipeline, the top of the quantitative container is provided with the second vertical pipeline which is connected with the atmosphere, and the horizontal branch pipeline is also provided with the third vertical pipeline which is connected with the dispensing container at the position corresponding to the first vertical pipeline.

[0011] Preferably, the power mechanism is a gravity driving mechanism, that is, the position of the sample container is higher than that of the quantitative container, and the height position of the sample container is adjustable, or is a peristaltic pump arranged on the total conveying pipeline, or is a blowing mechanism for blowing gas into the sample container.

[0012] Preferably, the second vertical pipeline is connected with the first dropper through the first communication pipeline, the top of the first dropper is connected with the first gas filter, and the first communication pipeline is also provided with a first air pressure sensor, a fourth switch valve and a second gas filter which are sequentially arranged and connected with the first communication pipeline.

[0013] Preferably, the sample container is connected with a plurality of preparation containers through branch pipelines respectively, and each branch pipeline is provided with a third switch valve.

[0014] Preferably, when the power mechanism is a peristaltic pump arranged on the total conveying pipeline, the peristaltic pump outlet is connected with the horizontal section branch pipeline through a second communication pipeline, the fifth switch valve and the first bubble sensor are arranged on the second communication pipeline in sequence from the direction away from the peristaltic pump, the third gas filter, the second bubble sensor, the quantitative container and the sixth switch valve are arranged on the second communication pipeline in sequence and are in communication with the second communication pipeline, and the pipeline between the total switch valve and the peristaltic pump is provided with an exhaust pipeline, the seventh switch valve and the fourth gas filter are arranged on the exhaust pipeline in sequence, and the second drip cup is arranged on the exhaust pipeline.

[0015] The utility model also provides a kind of liquid quantitative subpackaging equipment, which is used in cooperation with the above subpackaging structure, comprising: main support and pipe clamp for fixing corresponding pipeline.

[0016] Preferably, the main support includes a first support portion for fixing the sample container and adjustable in height, and a second support portion arranged in parallel with the first support portion; a plurality of connecting plates are arranged between the first support portion and the second support portion, and a plurality of pipe clamps are arranged on the connecting plates; the first switch valve includes a first fixed plate arranged on the back of the horizontal section branch pipeline and a first pressing plate arranged on the front of the horizontal section branch pipeline, and a plurality of protrusions for pressing the horizontal section branch pipeline are arranged on the first pressing plate; the second switch valve includes a second fixed plate arranged on the back of the third vertical pipeline and a second pressing plate arranged on the front of the third vertical pipeline; a pressing mechanism for pressing the corresponding pressing plate and fixed plate is arranged on the first pressing plate and the second pressing plate; and the first fixed plate and the second fixed plate are arranged on the main support.

[0017] Preferably, the first fixed plate and the second fixed plate are integrally formed or separately arranged; the pressing mechanism is a connecting rod pressing mechanism; the first pressing plate and the second pressing plate are detachably connected with the pressing mechanism; a gasket is arranged at the end of the protrusion; a fixing portion for fixing the drip cup is further arranged on the main support; the first support portion and the second support portion are fixedly arranged on a base; and support legs for adjusting the levelness are arranged under the base.

[0018] Preferably, the main support includes a support table, a housing frame and a fixing support for fixing the quantitative tube and the subpackaging container; the housing frame is divided into a first cavity and a second cavity; the first cavity is a heat preservation box, the sample container is arranged in the heat preservation box in a liftable manner, and a pressing plate for pressing the sample container is further arranged in the first cavity; and a display screen and switch valve control buttons are arranged on the front sidewall of the second cavity.

[0019] Preferably, the fixing support is a beam type, comprising horizontally arranged upper beam support and lower beam support, one end of the upper beam support and the lower beam support is hingedly connected with the shell frame, and the other end is a movable end; or a rotary type, comprising circular upper rotary frame, middle rotary frame, lower rotary frame and base arranged in sequence from top to bottom, the upper rotary frame and the middle rotary frame are connected through a plurality of supporting rods, and the middle rotary frame and the lower rotary frame are connected through a plurality of supporting rods, and the lower rotary frame and the base are connected through a rotary bearing.

[0020] The positive effects of the utility model are as follows:

[0021] (1) The utility model discloses a vertical pipeline in communication with the atmosphere is arranged on the top of the quantitative container, when the liquid in the sample container is distributed to each quantitative container by using the principle of communicating vessel, the vertical pipeline can allow the liquid distributed to the quantitative container to overflow the quantitative container in a small amount, so that the liquid column stops at the vertical pipeline, and since the inner cross-sectional area of the vertical pipeline is much smaller than the inner cross-sectional area of the quantitative container, the error ΔV of the distribution precision is greatly reduced, and experiments prove that the distribution error of the structure can be controlled within 0.2%, compared with the precision error of 2% of the existing distribution equipment, the distribution precision is greatly improved.

[0022] (2) The height of the sample container can be adjusted, and the height difference ΔH between the sample container and the quantitative container can be adjusted as required, the greater the ΔH, the greater the hydraulic pressure difference, the greater the power provided during distribution, the faster the liquid flows during distribution, and the higher the distribution efficiency, that is, the power and the distribution efficiency of the patent can be controlled as required. In addition, the patent also supports adding a peristaltic pump or other power device below the sample container, so as to provide distribution power by using the peristaltic pump or other device.

[0023] (3) The switch valve structure provided by the utility model can open and close multiple pipelines at one time, when the number of quantitative containers and distribution containers during distribution is large, the switch valve structure can greatly simplify the opening and closing operation of multiple pipelines, effectively improve the distribution efficiency, and reduce the risk of misoperation.

[0024] (4) The utility model adopts a fully enclosed pipeline consumable, can realize fully enclosed distribution, and better avoids the external pollution of the distribution liquid.

[0025] (5) On the basis of ensuring the accuracy of the sub-packaging, the utility model discloses can realize the fast sub-packaging of multiple bags simultaneously and the controllable speed, and through the test use, for the sub-packaging of 10ml / bag, can realize 100 bags sub-packaging in 10min (the whole operation process time). And such as the patent technology such as CN113022911A, CN118665799A, CN118665798A, for the sub-packaging of 10ml / bag, at least needs 1 bag in average 1 minute, and 100 bags need about 100 minutes, and compared with the prior art, the utility model has the remarkable improvement (efficiency improves ten times) in the sub-packaging efficiency while ensuring the accuracy of the sub-packaging.

[0026] (6) The utility model discloses can carry out the exhaust operation to the sub-packaging container after the sub-packaging, avoids the residual air in the sub-packaging container (the residual air is not favorable for the subsequent cryopreservation of cell).

[0027] In summary, compared with the prior art, the utility model can greatly improve the sub-packaging accuracy, and can also control the sub-packaging power and the sub-packaging efficiency as needed, and the sub-packaging operation is simple, the operation efficiency is high, the sub-packaging is fully closed, and is especially suitable for the sub-packaging application of biological preparations such as cells. BRIEF DESCRIPTION OF DRAWINGS

[0028] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, and obviously, the drawings in the following description are only the embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to the provided drawings without creating the creative labor.

[0029] Figure 1 It is the structure schematic drawing of the liquid ration sub-packaging system provided by the first specific embodiment of the utility model;

[0030] Figure 2 It is the structure schematic drawing of the liquid ration sub-packaging system provided by the second specific embodiment of the utility model;

[0031] Figure 3 It is the structure schematic drawing of the liquid ration sub-packaging system provided by the third specific embodiment of the utility model;

[0032] Figure 4 It is the structure schematic drawing of the liquid ration sub-packaging system provided by the fourth specific embodiment of the utility model;

[0033] Figure 5 It is the structure schematic drawing of the mixed sub-packaging structure provided by the fifth specific embodiment of the utility model;

[0034] Figure 6It is the structure schematic view of liquid quantitative subpackaging system provided by the sixth specific embodiment of the utility model;

[0035] Figure 7 It is the liquid column stop position schematic view when the quantitative container is not filled;

[0036] Figure 8 It is the liquid column stop position schematic view when the liquid overflows the quantitative container;

[0037] Figure 9 It is the structure schematic view of liquid quantitative subpackaging equipment when being pressed of the seventh specific embodiment of the utility model;

[0038] Figure 10 It is the structure schematic view of the A area of the utility model; Figure 9

[0039] It is the structure schematic view of the B area of the utility model; Figure 11 Figure 9 It is the structure schematic view of the C area of the utility model;

[0040] Figure 12 Figure 9 It is the structure schematic view of the liquid quantitative subpackaging system when not being pressed of the seventh specific embodiment of the utility model;

[0041] Figure 13 It is the structure schematic view of the liquid quantitative subpackaging system when not being pressed of the seventh specific embodiment of the utility model;

[0042] Figure 14 It is the structure schematic view of the liquid quantitative subpackaging system (before liquid subpackaging) of the eighth specific embodiment of the utility model;

[0043] Figure 15 It is the structure schematic view of the liquid quantitative subpackaging system (in the process of liquid distribution to the quantitative pipe) of the eighth specific embodiment of the utility model;

[0044] Figure 16 It is the structure schematic view of the liquid quantitative subpackaging system (after liquid completely distributes to the quantitative pipe) of the eighth specific embodiment of the utility model;

[0045] Figure 17 It is the structure schematic view of the liquid quantitative subpackaging system (in the process of liquid subpackaging from the quantitative pipe to the subpackaging bag) of the eighth specific embodiment of the utility model;

[0046] Figure 18 It is the structure schematic view of the liquid quantitative subpackaging system of the ninth specific embodiment of the utility model;

[0047] Figure 19 It is the structure schematic view of the liquid quantitative subpackaging system of the tenth specific embodiment of the utility model; ​​

[0048] Figure 20 is the structure schematic view of the liquid quantitative sub-packaging equipment provided by the eleventh specific embodiment of the utility model;

[0049] Figure 21 is the structure schematic view of the rotary fixing support provided by the twelfth specific embodiment of the utility model;

[0050] Figure 22 is the structure schematic view of the liquid quantitative sub-packaging equipment provided by the thirteenth specific embodiment of the utility model.

[0051] The reference signs are as follows:

[0052] Sample container 1, quantitative container 2, sub-packaging container 3, first dropper 4, first gas filter 5, liquid column stop position 6, total conveying pipeline 7, horizontal section branch pipeline 8, first vertical pipeline 9, second vertical pipeline 10, third vertical pipeline 11, first communication pipeline 12, first on-off valve 13, second on-off valve 14, multi-way joint 16, third on-off valve 17, preparation container 18, peristaltic pump 19; eighth on-off valve 20, first air pressure sensor 21, fourth on-off valve 22, second gas filter 23, first air bubble sensor 24, fifth on-off valve 25, sixth on-off valve 26, quantitative pipe 27, second air bubble sensor 28, third gas filter 29, seventh on-off valve 30, second dropper 31, fourth gas filter 32, fourth gas filter 33, ninth on-off valve 34, air pump 35;

[0053] First support part 201, second support part 202, fixed plate 203, first pressing plate 204, second pressing plate 205, pressing mechanism 206, base 207, support leg 208, connecting plate 209, hanging rod 210, hook 211, height adjuster 212, fixed part 213, pipe clamp 214, base 215, first connecting rod 216, second connecting rod 217, driving rod 218, handle 219, protruding block 220;

[0054] Supporting table 301, shell frame 302, extrusion plate 303, heat preservation box 304, cross rod 305, hook 306, first bubble sensor fixed position 307, display screen 308, first air pressure sensor fixed position 309, fourth switch valve control key 310, sixth switch valve control key 311, second bubble sensor fixed position 312, fifth switch valve control key 313, second drop funnel fixed frame 314, peristaltic pump installation position 315, fixed frame 316 of quantitative tube, total switch valve control key 317, seventh switch valve control key 318, upper cross beam support 319, lower cross beam support 320, first drop funnel fixed frame 321, upper rotating frame 322, middle rotating frame 323, lower rotating frame 324, base 325, rotating bearing 326, supporting leg 327, drop one drop funnel fixed frame 328, third cavity 329, supporting rod 330. DETAILED DESCRIPTION

[0055] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0056] With reference to Figure 1 The utility model provides a kind of liquid quantitative subpackaging system, including sample container 1, several quantitative containers 2, subpackaging container 3, switch valve and power mechanism for driving the liquid in sample container to flow into quantitative container, the bottom of the sample container 1 is connected with total delivery pipeline 7, this total delivery pipeline 7 has several horizontal section branch pipeline 8, the horizontal section branch pipeline 8 is connected with the bottom of corresponding quantitative container 2 by first vertical pipeline 9, the top of the quantitative container 2 is equipped with second vertical pipeline 10 that is connected with atmosphere, the horizontal section branch pipeline 8 is also equipped with third vertical pipeline 11 that is connected with subpackaging container 3 with the position corresponding to first vertical pipeline 9;The switch valve includes first switch valve 13 for the horizontal section branch pipeline between adjacent first vertical pipeline 9 is opened and closed, second switch valve 14 for the third vertical pipeline 11 is opened and closed and total switch valve 15 for the total delivery pipeline 7 is opened and closed;The power mechanism of the embodiment is gravity drive mechanism, and the position of the sample container 1 is higher than quantitative container 2.

[0057] The sample container 1 is used to hold the liquid to be dispensed. It can be any container capable of holding liquid, such as a sample bag or sample box. This invention preferably uses a sample bag. Similarly, the quantitative container 2 can be any form, such as a quantitative tube, with the bottoms of all quantitative tubes at the same level. Since the sample container (sample bag) is higher than the quantitative container (quantitative tube), when the main switch is turned on, the hydraulic pressure created by the height difference will cause the sample liquid to flow towards each quantitative tube, while simultaneously feeding liquid into the quantitative tube. When the liquid in the sample bag has finished flowing down, according to the principle of communicating vessels, the liquid columns in each quantitative tube branch stop at the same level, thus enabling the sample solution to be evenly distributed into several quantitative containers at the same time, thereby achieving rapid dispensing.

[0058] In actual operation, due to factors such as the levelness of the surrounding ground and the levelness of the dispensing equipment, each quantitative container cannot achieve absolute levelness. The height difference (Δh) of the liquid in each quantitative tube after dispensing will cause a dispensing accuracy error ΔV, where ΔV = Δh * S, and S is the cross-sectional area of ​​the liquid column. Figure 7 As shown, when the metering tube is not full, the cross-sectional area of ​​the liquid column is equal to the internal cross-sectional area S1 of the metering tube, i.e., S = S1. To reduce the error in dispensing accuracy, when making the dispensing plan, a metering container with a volume slightly smaller than the volume of liquid to be dispensed is selected. Because this invention has a vertical pipe connected to the atmosphere at the top of the metering container, after the liquid in the sample container is distributed to each metering container using the principle of communicating vessels, a small amount of liquid in the metering container overflows, thus causing the liquid column to stop at position 6 in the vertical pipe (e.g., ...). Figure 8 As shown in the figure, since the inner cross-sectional area of ​​the vertical pipe is much smaller than that of the quantitative container, the dispensing accuracy error ΔV is greatly reduced. Experimental verification shows that the dispensing error can be controlled within 0.2%, which is a significant improvement in dispensing accuracy compared to the 2% accuracy error of existing dispensing equipment.

[0059] Preferably, such as Figure 1 As shown, the second vertical pipe is connected to a first dripping bucket 4 via a first connecting pipe 12, and the top of the first dripping bucket 4 is connected to a first gas filter 5. The first dripping bucket 4 serves as a buffer (if the liquid flows too quickly from the metering tube to the first gas filter 5, it may clog the first gas filter 5 and contaminate the cell preparation; therefore, a dripping bucket is placed between the metering pipe and the first gas filter 5 to prevent the liquid level from rising too quickly and reaching the filter). The first gas filter 5 prevents contamination of the dispensed liquid during the dispensing process.

[0060] Furthermore, the volume specifications of each quantitative container 2 can be the same or different (e.g., Figure 2As shown, the specification of the quantification container 2 can be self-adapted according to the requirement, such as 5-100ml specification, etc. In the same batch of dispensing process, if the quantification containers 2 with the same specification are selected, the communicating vessel can be used to realize the equal division, if the quantification containers 2 with different specifications are selected, the quantification and dispensing are carried out according to the volume of the quantification tube, such as Figure 2 As shown, 60ml sample solution is dispensed into 5 liquid bags, 20ml is dispensed into the No.2 bag, then the No.2 quantification tube is selected with the capacity specification of 20ml, 10ml is dispensed into the other 4 liquid bags, then the corresponding quantification tube is selected with the capacity specification of 10ml.

[0061] Preferably, as shown, a plurality of cross-shaped four-ways can be provided so that the horizontal section branch pipeline is respectively connected with the first vertical pipeline 9 above and the third vertical pipeline 11 below. Figure 1

[0062] Further, the horizontal section branch pipeline 8 can be one way or multiple ways, that is, the utility model can adapt to the dispensing requirement of dispensing any number of bags at one time. For example, as shown in the single-way horizontal section branch pipeline, 1 to n groups of quantification tubes (n is an integer greater than 1) can be connected; as shown in the multiple-way horizontal section branch pipeline, m groups of quantification tubes (m is an integer greater than 1) can be connected. Figure 1 Figure 3 In addition, a multi-way joint 16 can be further provided on the conveying pipeline below the sample bag to obtain the multi-way horizontal section branch pipeline which is star-shaped expanded in multiple horizontal directions, such as m direction expansion (m is an integer greater than 1), then the dispensing of m*n bags of liquid can be simultaneously realized; in addition, star+star type expansion can be carried out, as shown. Figure 4

[0063] As shown, the sample container 2 can also be connected with a plurality of preparation containers 18 through branch pipelines, different liquid preparations can be contained in each preparation container 18, and a third switch valve 17 is arranged on each branch pipeline to realize the mixed dispensing of multiple different liquid reagents. Figure 5

[0064] Preferably, the position of the sample container 1 is adjustable relative to the height of the position of the quantification container 2. As shown, since the hanging position of the sample bag is higher than that of the quantification tube, the liquid height difference ΔH can be used to provide power for the dispensing, the greater the ΔH, the greater the hydraulic pressure difference, the faster the liquid flow during the dispensing, and the higher the dispensing efficiency. That is, the utility model can control the speed of the dispensing by adjusting the position height of the sample container 1, thereby realizing the control of the dispensing power and the dispensing efficiency. In addition, since the dispensing speed can be controlled by adjusting the hanging height of the sample bag, the corresponding relationship table between the sample bag height and the dispensing flow rate can be sorted out through calculation and a large number of experimental records, and the appropriate dispensing speed can be selected according to the actual situation during the specific dispensing. Figure 1

[0065] ​​​​​In addition, a peristaltic pump or other device can be added below the sample container 2, such as a peristaltic pump 19 (as shown in Figure 6 ) provided on the total conveying pipeline, that is, the utility model can also use a peristaltic pump or other device to provide a split charging power.

[0066] Referring to Figure 9 , an embodiment of the utility model provides a kind of liquid ration split charging equipment, which is used to cooperate with the above split charging structure, comprising: main support and the pipe card 214 for fixing corresponding pipeline.Wherein, main support can be used to fix components such as sample container 1, ration container 2, split charging container 3, first dropper 4;Pipe card 214 can be used to fix each pipeline, ensure that pipeline keeps vertical or horizontal state during split charging process.

[0067] Specifically, as shown in Figure 9 And Figure 10 , the main support includes first support part 201 for fixing the sample container and height adjustable, and second support part 202 arranged in parallel with the first support part;A plurality of connecting plates 209 are provided between the first support part and the second support part, and a plurality of pipe cards 214 are provided on the connecting plates 209.As shown in Figure 10 , preferably, the first support part 201 can be in the form of a support rod, and the support rod can also be provided in a telescopic form;The top end of the support rod can be provided with a hanging rod 210, which is connected to the support rod by a height adjuster 212, and a plurality of hooks 211 can be provided on the hanging rod 210 for hanging sample bags and other sample containers;The height adjuster 212 can be a slider provided on the support rod and movable up and down along the support rod, and a locking mechanism such as locking bolt is provided on the slider to limit the movement of the slider.

[0068] Preferably, as shown in Figure 9 And Figure 13 , the first switch valve 13 includes a first fixed plate 203 arranged on the back of the horizontal section branch pipeline and a first pressing plate 204 arranged on the front of the horizontal section branch pipeline, and a plurality of protrusions 220 are provided on the first pressing plate 204 for extruding the horizontal section branch pipeline;The second switch valve 14 includes a second fixed plate arranged on the back of the third vertical pipeline and a second pressing plate 205 arranged on the front of the third vertical pipeline;The first pressing plate 204 and the second pressing plate 205 are both provided with a pressing mechanism 206 for pressing each other between the corresponding pressing plate and the fixed plate;The first fixed plate and the second fixed plate are both arranged on the main support.

[0069] Further, the first fixed plate and the second fixed plate are integrally formed (i.e. a plate, as shown in Figure 9The fixed plate is divided into two parts, one part is a first fixed plate, and the other part is a second fixed plate. The first fixed plate and the second fixed plate are arranged in a split manner (i.e. two plates arranged separately). Above the first fixed plate is a quantitative distribution area for installing quantitative tubes; below the second fixed plate is a liquid bag area for installing hanging liquid bags.

[0070] Preferably, the pressing mechanism 206 is a connecting rod pressing mechanism, and of course, other pressing mechanisms capable of moving the pressing plate towards the fixed plate and away from the fixed plate can also be used. Specifically, the connecting rod pressing mechanism has a structure as shown in the following figure: Figure 12 The structure of the connecting rod pressing mechanism includes a base 215, a driving rod 218, a first connecting rod 216, and a second connecting rod 217. The base 215 is arranged on the fixed plate, and the base 215 has an L-shaped top surface. One end of the first connecting rod 216 is hinged to one end of the L-shaped top surface, and the other end of the first connecting rod 216 is hinged to the driving rod 218. The second connecting rod 217 is L-shaped, one end of which is hinged to one end of the L-shaped top surface, the corner end is hinged to the end of the driving rod 218, and the other end is hinged to the corresponding pressing plate. The driving rod 218 is provided with a handle 219 for easy operation. When the first pressing plate 204 is not pressed against the first fixed plate (i.e. when the horizontal branch pipeline is in an open state), as shown in the following figure, the first pressing plate 204 and the first fixed plate are in a parallel state. When the handle 219 is pressed towards the first fixed plate, the first pressing plate 204 gradually moves towards the vertical direction of the fixed plate under the action of the connecting rods. When the first pressing plate 204 is perpendicular to the fixed plate, as shown in the following figure, the protrusions 220 on the first pressing plate 204 press and close the corresponding horizontal branch pipeline. Preferably, the end of the protrusion 220 can be provided with a gasket to assist the pressing block in pressing the corresponding pipeline. Figure 13 Figure 9 Figure 13 Figure 9

[0071] Preferably, the first pressing plate 204 and the second pressing plate 205 are detachably connected to the pressing mechanism 206, such as shown in the following figure, where the pressing plate is detachably connected to the second connecting rod. The detachable connection can be a bolt connection or the like. Figure 12

[0072] Figure 9 Figure 11 ​​​​​​​As shown, the main body support is further provided with a fixing part 213 for fixing the dropping funnel; the first support part 201 and the second support part 202 are fixedly arranged on a base 207; and the base 207 is provided with adjustable supporting legs 208.

[0073] Figure 14 to Figure 17 The liquid quantitative subpackaging system and the corresponding subpackaging method provided by the embodiment of the utility model, assuming that there is a sample solution of 1000ml, which needs to be evenly subpackaged into 50 cryopreservation bags, and each bag is subpackaged with 20ml, and the specific subpackaging operation steps are as follows:

[0074] (1) Subpackaging pipeline structure installation

[0075] Take a set of closed pipeline consumables containing 50 20ml quantitative pipes, connect 50 100ml cryopreservation bags (subpackaging containers) to the pipeline below each quantitative pipe (i.e. the third vertical pipeline) through a sterile connecting pipe, install each pipeline consumable (the subpackaging system) on the subpackaging equipment, and ensure that all the quantitative pipes are in the same horizontal position.

[0076] (2) Preparation of liquid agent to be subpackaged

[0077] Prepare a 1L sample liquid bag, and take 100ml of cell liquid, 300ml of human white solution and 600ml of cryopreservation solution according to the ratio of 1:3:6 and add them into the sample liquid bag in sequence.

[0078] (3) Cell liquid mixing

[0079] Put the sample bag containing the cell liquid on a shaker, mix thoroughly for 5 minutes, connect the sample liquid bag to the main conveying pipeline through a sterile connecting pipe, and adjust to the set height.

[0080] (4) Siphon equal division

[0081] Close the second switch valve to close each third vertical pipeline, open the first switch valve to make the horizontal branch pipeline between adjacent first vertical pipelines in an open state, and open the sample bag total switch valve. Due to the height difference, the cell liquid in the sample container flows quickly into the 50 quantitative pipes (as shown in Figure 15 ), each quantitative pipe realizes equal division based on the siphon principle, and when the equal division is completed and each liquid column stops at the same position (as shown in Figure 16 ), the total switch valve is closed.

[0082] (5) Transfer into the bag

[0083] Close the first switch valve to make the horizontal branch pipeline between adjacent first vertical pipelines in a closed state, open the second switch valve to open each third vertical pipeline, and the cell liquid in the quantitative pipe flows downward into the corresponding cryopreservation bag under the action of gravity.

[0084] (6) Heat-sealed bag

[0085] Manually squeeze to expel the air from each individual packaging bag, then heat seal and remove.

[0086] Reference Figure 18 This specific embodiment provides a liquid quantitative dispensing system, including a sample container 1 and several quantitative containers ( Figure 18 The middle section contains a quantitative tube, a dispensing container, a switching valve, and a power mechanism for driving the liquid in the sample container into the quantitative container. Figure 1 The difference between the embodiments shown is that, Figure 18 The power mechanism in the illustrated embodiment is a peristaltic pump 19 on the main delivery pipeline 7. Simultaneously, the first connecting pipeline 12 is also equipped with a first pressure sensor 21, a fourth switching valve 22, and a second gas filter 23, which are sequentially connected to it. The outlet of the peristaltic pump 19 is connected to the horizontal branch pipeline via a second connecting pipeline. On the second connecting pipeline, starting from the direction away from the peristaltic pump 19, a fifth switching valve 25 and a first bubble sensor 24 are sequentially arranged. The second connecting pipeline is also equipped with a third gas filter 29, a second bubble sensor 28, a metering tube 27, and a sixth switching valve 26, which are sequentially arranged from far to near. An exhaust pipeline is also provided between the main switching valve 15 and the peristaltic pump 19, and this exhaust pipeline is sequentially equipped with a seventh switching valve 30, a second drip chamber 31, and a fourth gas filter 32.

[0087] The specific usage process is as follows: First, open the main switch valve 15 and the sixth switch valve 26. The peristaltic pump 19 pumps the liquid in the cell bag (sample container 1) into the quantitative tube 27. The peristaltic pump stops immediately after the second bubble sensor 28 detects the liquid. Calculate the liquid volume L = V / n per rotation of the peristaltic pump using the volume V of the quantitative tube 27 and the number of rotations n of the peristaltic pump. This completes the peristaltic pump calibration step and is used to achieve precise liquid volume control of the peristaltic pump (the peristaltic pump, in conjunction with the bubble sensor, achieves high-precision quantitative transmission). Then, open the fourth switch valve 22, the fifth switch valve 25, the main switch valve 15, the first bubble sensor 24, and all first switch valves 13 to control the peristaltic pump to pump the cell fluid in the cell bag into the quantitative tubes 1-30 (if the liquid in the cell bag is not completely pumped in, it can be pumped through the peristaltic pump). The pump precisely controls the total volume of the dispensed cell solution. Complete dispensing requires the second bubble sensor 24 to detect the presence of bubbles. Once sufficient liquid has been dispensed, the peristaltic pump stops and the corresponding valves are closed, completing the peristaltic pump transport of the cell solution. Waiting for the liquid levels in the metering tubes 1-30 to reach the same height (and stop at the same liquid column position), all first valves 13 are closed, and all second valves 14 are opened. After all the cell solution in the metering tubes 1-30 has flowed into the bag 1-30, all second valves 14 are closed, completing the quantitative dispensing of the cell solution. If there is still a large amount of cell solution in the cell bag that needs to be dispensed, the tubing on the right side of the second bubble sensor 24 can be replaced with a set of tubing consumables on the right side using a sterile connector, allowing for continued quantitative dispensing.

[0088] After each dispensing of the filling bag is completed, air may remain in the liquid bag. The seventh switch valve 30, the fifth switch valve 25, all the first switch valves 13 and the second switch valve 14, and the first pressure sensor 21 can be opened. The eighth switch valve 20 below the first dripping bucket 4, the main switch valve 15, the sixth switch valve 26, and the fourth switch valve 22 can be closed. The peristaltic pump is controlled to rotate to expel the gas in the liquid bag 1-30 and the metering tube 1-30. The first pressure sensor 21 is used to determine whether the gas has been completely expelled (when the air is completely expelled, a negative pressure will be formed in the pipeline, such as -0.1 kPa, which indicates that the air in the liquid bag has been completely expelled).

[0089] Reference Figure 19 This specific embodiment provides a liquid quantitative dispensing system, including a sample container 1 and several quantitative containers ( Figure 18 The middle section contains a quantitative tube, a dispensing container, a switching valve, and a power mechanism for driving the liquid in the sample container into the quantitative container. Figure 1 The difference between the embodiments shown is that, Figure 19The power mechanism of the embodiment shown is a gas blowing mechanism for blowing gas into the sample container 1. As an example, the gas blowing mechanism is a gas pump 35 (in the form of a peristaltic pump) in communication with the sample container, and a ninth on-off valve 34 and a fourth gas filter 33 are provided in communication with the gas pump 35. When the power mechanism in this form is used, the ninth on-off valve 34, the total on-off valve 15 and the gas pump 35 are opened, and gas is blown into the sample container by the gas pump. When the pressure in the sample container reaches a certain level, the cell solution is pushed into the quantitative tube 1-30, and the subsequent steps are the same as described above.

[0090] With reference to Figure 20 The embodiment provides another liquid quantitative dispensing device, which is used in cooperation with the dispensing structure and includes a main support and a tube clamp for fixing corresponding pipelines. The main support includes a support table 301, a housing frame 302 and a fixing support for fixing the quantitative tube and the sample container. The internal cavity of the housing frame 302 is divided into a first cavity and a second cavity. The first cavity is a heat preservation box 304. The sample container is arranged in the heat preservation box in a liftable manner. An extrusion plate 303 for extruding the sample container is arranged in the first cavity. Specifically, the heat preservation box 304 is of an automatic temperature control type. A hook 306 for hanging the sample container 1 is arranged in the heat preservation box 304. The hook 306 is arranged on a horizontal rod 305. The two ends of the horizontal rod 305 are connected to vertical frames in a liftable manner. At the same time, the extrusion plate 303 is arranged in the heat preservation box 304. The extrusion plate can reciprocate in a direction perpendicular to the sample container (for example, an electric sliding table mechanism can be used to realize the reciprocation of the extrusion plate). When the device is used, the cell bag is first hung on the hook. The height of the horizontal rod 305 is adjusted so that the cell solution bag is at the position of the extrusion plate (the liftable horizontal rod can be adapted to different sizes of cell bags). A temperature control module (for example, a temperature of 4°C with an accuracy of ±0.5°C) is arranged to realize constant temperature storage of the cell bag. The reciprocation of the extrusion plate is started to continuously extrude the sample container, so that the cells in the sample container 1 are uniformly mixed.

[0091] Further, a display screen 308, on-off valve control buttons, sensor fixing clamps and the like are arranged on the front side wall of the second cavity. For example, a first gas pressure sensor fixing position 309, a fourth on-off valve control key 310, a first bubble sensor fixing position 307, a second bubble sensor fixing position 312, a quantitative tube 27 fixing frame 316, a sixth on-off valve control key 311, a fifth on-off valve control key 313, a total on-off valve control key 317, a seventh on-off valve control key 318, a second dropper fixing frame 314 and a peristaltic pump mounting position 315 are arranged on the front side wall of the second cavity. Figure 18 The first gas pressure sensor fixing position 309, the fourth on-off valve control key 310, the first bubble sensor fixing position 307, the second bubble sensor fixing position 312, the quantitative tube 27 fixing frame 316, the sixth on-off valve control key 311, the fifth on-off valve control key 313, the total on-off valve control key 317, the seventh on-off valve control key 318, the second dropper fixing frame 314 and the peristaltic pump mounting position 315 are arranged on the front side wall of the second cavity.

[0092] As Figure 20As shown, in a preferred embodiment, the fixing bracket is a beam type, including a horizontally arranged upper beam bracket 319 and a lower beam bracket 320. One end of the upper beam bracket 319 and the lower beam bracket 320 is hinged to the side wall of the shell frame, and the other end is a movable end. Several tube clamps can be installed on both the upper beam bracket 319 and the lower beam bracket 320. The upper beam bracket 319 is equipped with a fixing frame 321 for fixing the first dripping bucket. The upper beam bracket 319 is used to fix the position above the metering tube, and the lower beam bracket 320 is used to fix the position below the metering tube. When not in use, the upper beam bracket 319 and the lower beam bracket 320 can be folded down to fit tightly against the side wall of the shell frame, thereby reducing the space occupied by the device.

[0093] like Figure 21 As shown, in another preferred embodiment, the fixed support is rotatable, comprising, from top to bottom, an upper circular rotating frame 322 (which can be configured as an annular ring), a middle rotating frame 323, a lower rotating frame 324, and a base 325. The upper rotating frame 322 and the middle rotating frame 323, and the middle rotating frame 323 and the lower rotating frame 324, are connected by several support rods 330, respectively. The lower rotating frame 324 and the base 325 are connected by a rotary bearing 326. Preferably, a drip-feeder fixing frame 328 is also provided above the upper rotating frame 322, and several feet 327 are provided at the bottom of the base 325.

[0094] This rotating mounting bracket has a rotating bearing at its bottom, allowing the entire bracket to rotate 360 ​​degrees. Multiple mounting and positioning holes (the number can be set as needed) are evenly distributed circumferentially along the upper and lower rotating brackets. During the overall assembly of the modular structure, the piping consumables can be installed by rotating the rotating brackets. The upper rotating bracket secures the upper part of the metering tube, and the lower rotating bracket secures the lower part. Compared to other rotating structures... Figure 20 The beam-type structure shown can make more efficient use of space. More liquid bags can be hung in the same space. For example, the diameter of the rotating fixing bracket for 30 liquid bags is about 0.5 meters, while the length of the beam-type bracket when unfolded is about 1.2 meters (the beam-type fixing bracket can rotate and fit tightly against the equipment, which is suitable for dispensing a smaller number of liquid bags, such as 20 bags or less).

[0095] The rotating fixing bracket can be placed separately next to the dispensing equipment or placed inside the dispensing equipment, for example... Figure 22 The dispensing equipment shown is used for Figure 20 The difference in the illustrated embodiment is that the internal cavity of the housing frame 302 also includes a third cavity 329, in which the rotating fixing bracket can be placed for use.

[0096] In the description of the utility model, need understanding is, the orientation or position relation that the term "bottom", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "internal", "external" and the like indicate is based on the orientation or position relation shown in the drawing, only is for facilitating the description of the utility model and simplifying the description, and is not indicate or imply that the device or element indicated must have a particular orientation, construct and operate in a particular orientation, therefore can not be understood as the restriction of the utility model. In addition, the term "first", "second" and the like are only for the purpose of description, and can not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" and the like can explicitly or implicitly include one or more features. In the description of the utility model, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0097] The liquid quantitative sub-packaging system and sub-packaging equipment provided by the utility model are described in detail above, the principle and implementation mode of the utility model are described in this paper by applying specific examples, the description of the above examples is only for helping to understand the method and core idea of the utility model; at the same time, for the general technical personnel in the field, according to the idea of the utility model, the specific implementation mode and application range will have changes, so, the content of the specification should not be understood as the restriction of the utility model.

Claims

1. A liquid dosing system comprising a sample container, a number of dosing containers, a dispensing container, a switch valve and a power mechanism for driving the liquid in the sample container into the dosing containers, the sample container being connected to a common delivery line, characterized in that: The total conveying pipeline has several horizontal branch pipelines, the horizontal branch pipelines are connected with the bottoms of corresponding quantitative containers through first vertical pipelines, the tops of the quantitative containers are provided with second vertical pipelines in communication with the atmosphere, and the horizontal branch pipelines are also provided with third vertical pipelines connected with sub-packaging containers at positions corresponding to the first vertical pipelines; the switch valve comprises a first switch valve for opening and closing the horizontal branch pipelines between adjacent first vertical pipelines, a second switch valve for opening and closing the third vertical pipelines, and a total switch valve for opening and closing the total conveying pipeline.

2. The liquid dosing system according to claim 1, characterized in that: The power mechanism is a gravity driving mechanism, i.e. the position of the sample container is higher than that of the quantitative container and the height position of the sample container is adjustable; or a peristaltic pump arranged on the total conveying pipeline; or a pumping mechanism for pumping gas into the sample container.

3. The liquid dosing system according to claim 1 or 2, characterized in that: The second vertical pipeline is connected with a first dropper through a first communication pipeline, and the top of the first dropper is connected with a first gas filter; the first communication pipeline is also provided with a first air pressure sensor, a fourth switch valve and a second gas filter arranged in sequence and connected with the first communication pipeline.

4. The liquid dosing system according to claim 1 or 2, characterized in that: The sample container is connected with several preparation containers through branch pipelines, and each branch pipeline is provided with a third switch valve.

5. The liquid dosing system of claim 2, wherein: When the power mechanism is a peristaltic pump arranged on the total conveying pipeline, the outlet of the peristaltic pump is connected with the horizontal branch pipeline through a second communication pipeline, the second communication pipeline is provided with a fifth switch valve and a first bubble sensor arranged in sequence from the direction away from the peristaltic pump; the second communication pipeline is also provided with a third gas filter, a second bubble sensor, a quantitative container and a sixth switch valve arranged in sequence and connected with the second communication pipeline from the direction close to the peristaltic pump; an exhaust pipeline is arranged between the total switch valve and the peristaltic pump, and the exhaust pipeline is provided with a seventh switch valve, a second dropper and a fourth gas filter arranged in sequence.

6. A liquid dosing apparatus, characterized in that The device is used in cooperation with the liquid quantitative sub-packaging system in any one of claims 1-5, and comprises a main body support and pipe clamps for fixing corresponding pipelines.

7. The liquid dosing apparatus according to claim 6, characterized in that: The main body support comprises a first support part for fixing the sample container and adjustable in height, and a second support part arranged in parallel with the first support part; a plurality of connecting plates are arranged between the first support part and the second support part, and a plurality of pipe clamps are arranged on the connecting plates; the first switch valve comprises a first fixing plate arranged on the back of the horizontal branch pipeline and a first pressing plate arranged on the front of the horizontal branch pipeline, and a plurality of protrusions for pressing the horizontal branch pipeline are arranged on the first pressing plate; the second switch valve comprises a second fixing plate arranged on the back of the third vertical pipeline and a second pressing plate arranged on the front of the third vertical pipeline; a pressing mechanism for pressing the corresponding pressing plate and the fixing plate is arranged on the first pressing plate and the second pressing plate; and the first fixing plate and the second fixing plate are arranged on the main body support.

8. The liquid dosing apparatus according to claim 7, characterized in that: The first fixed plate and the second fixed plate are integrally formed or separately arranged; the pressing mechanism is a connecting rod pressing mechanism; the first pressing plate and the second pressing plate are detachably connected with the pressing mechanism; the end of the protruding block is provided with a gasket; the main support is further provided with a fixing part for fixing a dropping funnel; the first support part and the second support part are fixedly arranged on a base; the base is provided with supporting legs for adjusting the levelness.

9. The liquid dosing apparatus according to claim 6, characterized in that: The main support comprises a support table, a shell frame and a fixing support for fixing a dosing tube and a split container, the shell frame is internally partitioned into a first cavity and a second cavity; the first cavity is a heat preservation box, the sample container is arranged in the heat preservation box in a liftable manner, the first cavity is further provided with a pressing plate for pressing the sample container; the front sidewall of the second cavity is provided with a display screen and a switch valve control button.

10. The liquid dosing apparatus according to claim 9, characterized in that: The fixing support is a beam type, comprising horizontally arranged upper beam support and lower beam support, one end of the upper beam support and the lower beam support is hingedly connected with the shell frame, and the other end is a movable end; or is a rotary type, comprising circular upper rotary frame, middle rotary frame, lower rotary frame and base arranged in sequence from top to bottom, the upper rotary frame and the middle rotary frame, the middle rotary frame and the lower rotary frame are connected through a plurality of supporting rods respectively, and the lower rotary frame and the base are connected through a rotary bearing.

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