Low-concentration protein detection kit

Abstract

The utility model discloses a low-concentration protein detection kit, which is applied to the technical field of protein detection, and is characterized in that a protein liquid sample and a buffer solution are poured into a dilution tank, the protein liquid sample is diluted by the buffer solution, and then a micro pump is started to convey the diluted protein liquid into a mixing tank; then the genipin reagent solution is poured into the mixing tank to be mixed with the protein liquid, then the stirring assembly is started to stir the protein liquid, convenience and rapidness are achieved, and the concentration of protein in the sample solution is determined according to the final color of the solution in combination with a colorimetric card.

Description

TECHNICAL FIELD

[0001] The utility model belongs to protein detection technical field, especially relate to a low concentration protein detection kit. BACKGROUND

[0002] Protein concentration determination is a basic experimental content in protein biological technology experiment, and it is a basic prerequisite for experiments such as SDS-PAGE electrophoresis, Western Blot, Elisa, proteinase activity determination, protein two-dimensional electrophoresis, protein mass spectrometry, protein molecular weight determination and protein sequencing, and the commonly used protein concentration determination methods include acrylamide gel electrophoresis (isoelectric point method), peptide map method, terminal amino acid sequence detection, isoelectric focusing electrophoresis and the like.

[0003] With the rapid development of collagen protein related product market, higher requirements are put forward for the identification and detection method of collagen protein, including the simplicity, accuracy and sensitivity of detection. The collagen protein concentration of the collagen protein products sold in the market, especially gel and cream products, is often lower than 0.01%, so low concentration can only be detected by using expensive instruments and equipment, and it is urgent to develop a low-cost, rapid and efficient device for detecting the content of collagen protein. CONTENT OF THE UTILITY MODEL

[0004] The utility model discloses a low concentration protein detection kit, which can quickly realize low concentration protein detection and automatically stir protein liquid samples, and is convenient and fast.

[0005] The above technical purpose of the utility model is realized by the following technical scheme: a low concentration protein detection kit, comprising a box body, a box cover is rotatably connected to the top of the box body through a hinge, dilution grooves and mixing grooves are respectively formed in the inner walls of the box body, a stirring assembly is arranged in the box body, and a lock catch assembly is arranged on the inner wall of the box body.

[0006] The above technical scheme can uniformly mix protein liquid and reagent solution, is convenient and fast, and does not need manual operation.

[0007] The utility model is further provided with the following technical scheme: the bottom end of the connecting shaft is rotatably connected to the bottom of the inner cavity of the box body through a bearing seat.

[0008] The above technical scheme can stably rotate the connecting shaft.

[0009] The utility model further sets up, the box body includes first micro pump, draws liquid pipe, infusion tube, second micro pump, filter box and connecting pipe, the inside of box body is provided with first micro pump, the liquid end of first micro pump is provided with draws liquid pipe, the liquid end of first micro pump is provided with infusion tube, one end of draws liquid pipe penetrates the inner wall of dilution tank, one end of infusion tube penetrates the inner wall of mixing tank.

[0010] Adopt the above technical scheme: convenient to draw out the solution in dilution tank.

[0011] The utility model further sets up, one side of box body is provided with second micro pump, the liquid end of second micro pump penetrates the inner wall of dilution tank and is connected with filter box, one side of filter box is connected with connecting pipe, the inner wall of filter box is provided with filter paper.

[0012] Adopt the above technical scheme: convenient to filter sample solution.

[0013] The utility model further sets up, the top of box cover is provided with observation window, the bottom of box cover is provided with sealing strip.

[0014] Adopt the above technical scheme: improved the leakproofness after the closure of box body and box cover, and convenient to observe in box body.

[0015] The utility model further sets up, the lock catch subassembly includes the positioning rod of fixed in the inner wall of box body, the outside of positioning rod is connected with fixed block sliding, one side of fixed block is fixedly connected with spring, the top of fixed block is connected with limiting rod, the inner wall of box cover is seted up with through groove, the inner wall of through groove is seted up with limiting slot.

[0016] Adopt the above technical scheme: convenient to fix box cover on box body.

[0017] The utility model further sets up, the number of fixed block is two, one side of fixed block is fixedly connected with the lever of pushing.

[0018] Adopt the above technical scheme: convenient to move fixed block.

[0019] The utility model further sets up, the limiting rod and limiting slot joint.

[0020] Summarized above, the utility model has the following beneficial effects:

[0021] 1. Pour the protein liquid sample and buffer solution into the dilution tank, dilute the protein liquid sample through the buffer solution, then start the second micro pump to extract the diluted liquid, intercept the protein through the filter paper, retain the protein with high concentration in the dilution tank, then start the first micro pump to transport the protein liquid in the dilution tank to the mixing tank, pour the genipin reagent solution into the mixing tank to mix with the protein liquid, then start the stirring assembly to stir the protein liquid, which is convenient and fast, the concentration of the protein in the sample solution is determined according to the final color combined with the colorimetric card, and the concentration of the collagen protein can be detected without complex detection methods.

[0022] 2. The device does not need to use expensive instrument equipment, and the collagen protein can be identified only through simple chemical reaction and conventional detection means, so that the identification cost is reduced, and the device is more easy to popularize and apply.

[0023] 3. The technical operation process of the device is simple and clear, and the experimental period is short, so that the required time for identification is greatly shortened, and the device is particularly important for application scenes, such as quality control and product research and development, which need to quickly identify the collagen protein content. DETAILED DESCRIPTION

[0024] Figure 1 is the overall structure schematic view of the utility model;

[0025] Figure 2 is the structure schematic view of the stirring assembly of the utility model;

[0026] Figure 3 is the structure schematic view of the box cover and sealing strip of the utility model;

[0027] Figure 4 is the utility model Figure 3 A place structure amplification schematic view.

[0028] Fig. 1, box body; 101, first micro pump; 102, liquid suction pipe; 103, infusion tube; 104, second micro pump; 105, filter box; 106, connecting pipe; 2, box cover; 201, observation window; 202, sealing strip; 3, dilution tank; 4, mixing tank; 5, connecting plate; 6, motor; 7, driving wheel; 8, driven wheel; 9, connecting shaft; 10, impeller; 11, positioning rod; 12, fixed block; 13, spring; 14, limiting rod; 15, through groove; 16, limiting groove. DETAILED DESCRIPTION

[0029] The utility model will be further explained in detail in combination with the drawings.

[0030] Example 1:

[0031] Reference Figure 1 and Figure 2The utility model provides a low concentration protein detection kit, including box 1, the top of box 1 is connected with box cover 2 through the hinge rotation, the inner wall of box 1 is seted up dilution groove 3 and mixing groove 4 respectively, the inside of box 1 is provided with stirring subassembly, stirring subassembly includes the connecting plate 5 fixed in the inner chamber bottom of box 1, one side of connecting plate 5 is provided with motor 6, the output of motor 6 is fixedly connected with driving wheel 7, the outside of driving wheel 7 is engaged with from driving wheel 8, and the inner ring of from driving wheel 8 is fixedly connected with connecting shaft 9, and one end of connecting shaft 9 penetrates through the bottom of mixing groove 4 and is fixedly connected with impeller 10.

[0032] The capacity of the dilution groove 3 and the mixing groove 4 is several times of the capacity of the glass reagent bottle 60ML, so that the protein sample after detection can be at least packed into three reagent bottles, thereby improving the detection efficiency.

[0033] Reference Figure 2 The bottom end of the connecting shaft 9 is rotatably connected with the bottom of the inner cavity of the box 1 through a bearing seat.

[0034] Reference Figure 2 The box 1 comprises a first micro pump 101, a liquid suction pipe 102, a liquid delivery pipe 103, a second micro pump 104, a filter box 105 and a connecting pipe 106, the inside of the box 1 is provided with the first micro pump 101, the liquid suction end of the first micro pump 101 is provided with the liquid suction pipe 102, the liquid delivery end of the first micro pump 101 is provided with the liquid delivery pipe 103, one end of the liquid suction pipe 102 penetrates through the inner wall of the dilution groove 3, and one end of the liquid delivery pipe 103 penetrates through the inner wall of the mixing groove 4.

[0035] Reference Figure 2 One side of the box 1 is provided with the second micro pump 104, the liquid suction end of the second micro pump 104 penetrates through the inner wall of the dilution groove 3 and is communicated with the filter box 105, one side of the filter box 105 is communicated with the connecting pipe 106, and the inner wall of the filter box 105 is provided with filter paper.

[0036] Reference Figure 1 And Figure 3 The top of the box cover 2 is provided with an observation window 201, and the bottom of the box cover 2 is provided with a sealing strip 202.

[0037] The use process is briefly described as follows: the protein liquid sample and the buffer solution are poured into the dilution groove 3, the protein liquid sample is diluted through the buffer solution, then the second micro pump 104 is started to draw out the diluted liquid through the connecting pipe 106 and the filter box 105, the filter paper in the filter box 105 is used for intercepting the protein, so that the protein with high concentration is retained in the dilution groove 3, then the first micro pump 101 is started to draw out the protein liquid in the dilution groove 3 through the liquid suction pipe 102 and deliver the protein liquid to the mixing groove 4 through the liquid delivery pipe 103.

[0038] Then the genipin reagent solution is poured into the mixing tank 4 to mix with the protein solution, and the motor 6 is started to drive the driving wheel 7 to rotate the driven wheel 8, so that the connecting shaft 9 drives the wave wheel 10 to rotate, thereby facilitating the stirring of the protein solution, which is convenient and fast.

[0039] Example 2:

[0040] Reference Figure 1 、 Figure 3 and Figure 4 A low-concentration protein detection kit, comprising a box body 1, a box cover 2 is rotatably connected to the top of the box body 1 through a hinge, a locking assembly is arranged on the inner wall of the box body 1, the locking assembly comprises a positioning rod 11 fixed to the inner wall of the box body 1, a fixed block 12 is slidably connected to the outer side of the positioning rod 11, a spring 13 is fixedly connected to one side of the fixed block 12, a limiting rod 14 is connected to the top of the fixed block 12, a through groove 15 is formed in the inner wall of the box cover 2, a limiting groove 16 is formed in the inner wall of the through groove 15, the number of the fixed blocks 12 is two, a pull rod is fixedly connected to one side of the fixed block 12, and the limiting rod 14 is clamped with the limiting groove 16.

[0041] Brief description of the use process: move the pull rod to make the two fixed blocks 12 approach each other through the positioning rod 11, so that the spring 13 is compressed, then close the box cover 2, at this time the limiting rod 14 is located in the through groove 15, then release the pull rod, the two fixed blocks 12 drive the limiting rod 14 to automatically reset through the spring 13, and are clamped into the limiting groove 16, so that the box body 1 and the box cover 2 are fixed, and the operation is convenient.

[0042] The specific embodiment is only an explanation of the utility model, and is not a limitation of the utility model. Those skilled in the art can make modifications to the embodiment without creative contribution after reading the specification, but as long as the modifications are within the scope of the claims of the utility model, they are protected by the patent law.

Claims

1. A low concentration protein detection kit comprising a box (1), characterized in that: The top of the box body (1) is rotatably connected with a box cover (2) through a hinge, inner walls of the box body (1) are respectively provided with a dilution groove (3) and a mixing groove (4), capacities of the dilution groove (3) and the mixing groove (4) are several times of a 60ML capacity of a glass reagent bottle, an inside of the box body (1) is provided with a stirring assembly, and inner walls of the box body (1) are provided with a locking assembly. The stirring assembly comprises a connecting plate (5) fixed to a bottom of an inner cavity of the box body (1), one side of the connecting plate (5) is provided with a motor (6), an output end of the motor (6) is fixedly connected with a driving wheel (7), an outer side of the driving wheel (7) is meshingly connected with a driven wheel (8), an inner ring of the driven wheel (8) is fixedly connected with a connecting shaft (9), and one end of the connecting shaft (9) penetrates through the bottom of the mixing groove (4) and is fixedly connected with an impeller (10).

2. The low concentration protein detection kit according to claim 1, characterized in that: A bottom end of the connecting shaft (9) is rotatably connected with the bottom of the inner cavity of the box body (1) through a bearing seat.

3. The low concentration protein detection kit according to claim 1, characterized in that: The box body (1) comprises a first micro pump (101), a liquid suction pipe (102), a liquid delivery pipe (103), a second micro pump (104), a filter box (105) and a connecting pipe (106), the inside of the box body (1) is provided with the first micro pump (101), the liquid suction end of the first micro pump (101) is provided with the liquid suction pipe (102), the liquid delivery end of the first micro pump (101) is provided with the liquid delivery pipe (103), one end of the liquid suction pipe (102) penetrates through an inner wall of the dilution groove (3), and one end of the liquid delivery pipe (103) penetrates through an inner wall of the mixing groove (4).

4. The low concentration protein detection kit according to claim 3, characterized in that: One side of the box body (1) is provided with the second micro pump (104), the liquid suction end of the second micro pump (104) penetrates through the inner wall of the dilution groove (3) and is communicated with the filter box (105), one side of the filter box (105) is communicated with the connecting pipe (106), and an inner wall of the filter box (105) is provided with filter paper.

5. The low concentration protein detection kit according to claim 1, characterized in that: A top of the box cover (2) is provided with an observation window (201), and a bottom of the box cover (2) is provided with a sealing strip (202).

6. The low concentration protein detection kit according to claim 1, characterized in that: The locking assembly comprises a positioning rod (11) fixed to an inner wall of the box body (1), an outer side of the positioning rod (11) is slidably connected with a fixed block (12), one side of the fixed block (12) is fixedly connected with a spring (13), a top of the fixed block (12) is connected with a limiting rod (14), an inner wall of the box cover (2) is provided with a through groove (15), and an inner wall of the through groove (15) is provided with a limiting groove (16).

7. The low concentration protein detection kit according to claim 6, characterized in that: The number of the fixed blocks (12) is two, and one side of each fixed block (12) is fixedly connected with a push rod.

8. The low concentration protein detection kit according to claim 6, characterized in that: The limiting rod (14) is clamped with the limiting groove (16).

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