Rapid gel preparation device for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) gel electrophoresis

Abstract

The utility model relates to the technical field of SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) gel electrophoresis, and discloses a rapid gel preparation device for SDS-PAGE gel electrophoresis, which comprises a mixing pipe and a plurality of material storage pipes, a communication channel is fixedly arranged between the bottoms of the material storage pipes and the mixing pipe, plugging pieces are arranged in the material storage pipes, the plugging pieces are used for plugging the communication channel, and the material storage pipes are connected with the mixing pipe. A jacking part is slidably connected into the mixing pipe, and the jacking part can penetrate through the communicating channel and jack the plugging part upwards when sliding upwards along the inner wall of the mixing pipe, so that the storage pipe communicates with the mixing pipe. A plurality of material storage pipes are fixedly mounted at the top of the mixing pipe, the plugging pieces and the jacking pieces are arranged between the material storage pipes and the mixing pipe, and whether the material storage pipes are communicated with the mixing pipe or not can be controlled through the plugging pieces and the jacking pieces, namely whether raw material liquid is released into the mixing pipe or not to be mixed can be controlled; all the raw material liquid can be conveniently added into the mixing pipe for mixing at one time.

Description

TECHNICAL FIELD

[0001] The utility model relates to SDS -PAGE gel electrophoresis technical field, more specifically, especially, it is a kind of fast gel preparation device when SDS -PAGE gel electrophoresis. BACKGROUND

[0002] SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used protein separation technique, mainly used for separating according to the molecular weight of protein. Its principle is: molecular weight separation, SDS is an anionic detergent, which can combine with protein, so that protein carries consistent negative charge, thereby eliminating the original charge difference of protein. In this way, the mobility of protein in the electrophoresis process mainly depends on its molecular weight. Gel structure, SDS-PAGE usually adopts discontinuous gel system, including upper layer's concentrated gel and lower layer's separation gel. The concentrated gel has larger pore size, which is used to concentrate protein sample into narrow band, while the separation gel has smaller pore size, which is used to separate protein according to molecular weight.

[0003] Preparation of SDS protein gel needs to prepare A liquid, B liquid, etc. respectively, and in the preparation process, liquid gun is needed to add raw material liquid for many times, the operation is cumbersome, and after each solution is prepared by the traditional preparation method, liquid gun is used to drop on double-layer glass plate, the preparation solution and titration process are separated in this process, which takes a long time, and is not skilled in operation, which can cause the concentrated gel to solidify during gel preparation, and the gel cannot be poured. Polyacrylamide and methylenebisacrylamide are necessary chemical reagents for protein gel preparation, but they are also toxic substances. Generally, liquid gun is used to add solution to the glass plate, the liquid gun head is relatively thick, which can cause liquid outflow and glass plate breakage, and cause serious harm to the environment and human body.

[0004] Therefore, a convenient and fast gel preparation device capable of mixing raw material liquid at one time is needed. UTILITY MODEL CONTENT

[0005] The utility model aims at providing a kind of fast gel preparation device when SDS-PAGE gel electrophoresis, to solve the problems existing in prior art, several storage tubes are fixedly installed in the mixing pipe top in the device, and the storage tube is provided with plugging piece and jacking piece between the mixing pipe, whether the storage tube is communicated with the mixing pipe can be controlled by plugging piece and jacking piece, i.e. whether raw material liquid is released into mixing pipe for mixing can be controlled, raw material liquid is added into mixing pipe for mixing at one time.

[0006] To achieve the above object, the utility model provides the following scheme: the utility model provides a kind of fast gel preparation device when SDS-PAGE gel electrophoresis, comprising: mixing tube and several storage tubes, several The storage tube bottom is fixedly installed with intercommunication passage between the mixing tube, the storage tube is installed with obturator, the obturator is used to obturate the intercommunication passage, the mixing tube is slidably connected with jacking piece, the jacking piece can penetrate the intercommunication passage and jacking piece is jacked upward when sliding upward along the mixing tube inner wall, so that the storage tube is communicated with the mixing tube.

[0007] According to the fast gel preparation device when SDS-PAGE gel electrophoresis provided by the utility model, the obturator includes obturator ball and spring, one end of the spring is fixedly installed on the storage tube top wall, the other end is fixedly connected with the obturator ball, and the obturator ball is used to obturate the intercommunication passage.

[0008] According to the fast gel preparation device when SDS-PAGE gel electrophoresis provided by the utility model, the jacking piece includes slide rod, connecting rod and jacking column, the slide rod is slidably connected with the mixing tube inner wall at both ends, the connecting rod is fixedly connected on the slide rod, the jacking column is fixedly installed on the slide rod and the connecting rod, the jacking column corresponds to the position of the obturator ball, and the jacking column is used to jacking ball upward.

[0009] According to the fast gel preparation device when SDS-PAGE gel electrophoresis provided by the utility model, the mixing tube inner wall is provided with a sliding groove, and the slide rod slides along the sliding groove.

[0010] According to the fast gel preparation device when SDS-PAGE gel electrophoresis provided by the utility model, the slide rod is fixedly installed with magnetic block at both ends, the magnetic block is in contact with the sliding groove, and slides along the sliding groove.

[0011] According to the fast gel preparation device when SDS-PAGE gel electrophoresis provided by the utility model, the mixing tube bottom is provided with discharge port, and the discharge port is flat.

[0012] The utility model discloses the following technical effects:

[0013] The device is characterized in that a plurality of storage pipes are fixedly arranged on the top of the mixing pipe, different raw material components can be simultaneously added into the different storage pipes for storage, a communication channel is arranged between the storage pipe and the mixing pipe, and the opening or closing of the communication channel is controlled by the blocking piece arranged in the storage pipe and the jacking piece arranged in the mixing pipe; when the raw material liquid needs to be mixed to prepare glue, the jacking piece is controlled to rise to lift the blocking piece upward, so that the blocking piece is unblocked from the communication channel; at this time, the raw material liquid in the storage pipe can enter the mixing pipe simultaneously for mixing, which replaces the process of adding the raw material liquid multiple times by using the pipette for mixing in the prior art, and the device has the characteristics of simple and convenient operation and rapid glue mixing. BRIEF DESCRIPTION OF DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0015] Figure 1 It is a schematic diagram of the overall structure of the present application.

[0016] Figure 2 It is a sectional view of the overall structure of the present application.

[0017] Figure 3 It is a sectional view of another state in the present application.

[0018] Figure 4 It is a schematic diagram of the internal structure of the mixing pipe in the present application.

[0019] Figure 5 It is a schematic diagram of the structure of the jacking piece in the present application.

[0020] 1, storage pipe; 2, mixing pipe; 3, mixing bin; 4, discharge port; 5, chute; 6, blocking ball; 7, spring; 8, sliding rod; 9, jacking column; 10, connecting rod; 11, magnetic block. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0022] In order to make the above-mentioned purpose, characteristics and advantages of the utility model more apparent, obvious and easy to understand, the utility model will be explained in further detail below in combination with the drawings and specific embodiments.

[0023] As shown in Figures 1-5 The utility model provides a kind of fast gel preparation device when SDS-PAGE gel electrophoresis, comprising: mixing tube 2 and several storage tubes 1, several storage tubes 1 bottom and mixing tube 2 between fixed installation are connected with communication passage, storage tube 1 is installed with obturator, obturator is used to obturation to communication passage, obturator is slidably connected in mixing tube 2, and obturator can penetrate communication passage and be lifted up to obturator when being slid upward along mixing tube 2 inner wall, so that storage tube 1 is connected with mixing tube 2.

[0024] Different raw material liquids are placed in storage tube 1 respectively, mixing warehouse 3 is provided in mixing tube 2, for mixing different raw material liquids, obturator obturates communication passage, to prevent raw material liquid in storage tube 1 from flowing into mixing warehouse 3 in advance, obturator can be lifted up to obturator when being slid upward, at this time, obturator is away from communication passage, so that storage tube 1 is connected with mixing tube 2, and raw material liquid in different storage tubes 1 can flow into mixing warehouse 3 at this time simultaneously, and the process of mixing gel preparation is completed in mixing warehouse 3.

[0025] Obturator includes obturator ball 6 and spring 7, obturator ball 6 is fixedly installed below spring 7, and the top of spring 7 is fixedly connected to the inner top wall of storage tube 1, wherein the gravity of obturator ball 6 is greater than the tension of spring 7, in natural state, obturator ball 6 falls to communication passage, obturation is carried out to communication passage, to prevent raw material liquid in storage tube 1 from entering mixing tube 2 through communication passage, and the diameter of obturator ball 6 is greater than the diameter of communication passage.

[0026] Lift includes slide 8, connecting rod 10 and top column 9, slide 8 both sides are fixedly connected with connecting rod 10, and slide 8 and connecting rod 10 are all fixedly connected with top column 9, the length of slide 8 is greater than the length of connecting rod 10, and the width of slide 8, connecting rod 10 and top column 9 will not affect the flow of raw material liquid, and the position of top column 9 corresponds to the position of obturator ball 6, and top column 9 is used to lift obturator ball 6 upward.

[0027] Corresponding sliding slot 5 is provided in the inner wall of mixing tube 2, slide 8 is slid along sliding slot 5, and magnetic block 11 is fixedly connected at both ends of slide 8, and in the embodiment, magnetic block 11 is magnet, and magnetic block 11 is in sliding slot 5 and can slide up and down along sliding slot 5.Sliding slot 5 bottom supports magnetic block 11, to prevent lift from falling to the bottom of mixing tube 2.

[0028] When the lifting piece needs to be lifted, the two magnetic blocks 11 are respectively attached to the positions corresponding to the outer wall of the mixing pipe 2 and the sliding groove 5, and the magnetic blocks 11 are attracted by the opposite magnets, the two opposite magnets are simultaneously moved upward to drive the magnetic blocks 11 in the sliding groove 5 and the sliding groove 5 and the connecting rod 10 to move upward, the jacking column 9 penetrates the communication channel upward and contacts the blocking ball 6, and the blocking ball 6 is lifted upward to unblock the communication channel, at this time, the raw material liquid in different storage pipes 1 can flow into the mixing pipe 2 at the same time to mix and complete the glue making process. The pipe wall of the mixing pipe 2 will not affect the magnetic attraction force of the magnetic piece and the opposite magnet. The device can replace the process of using a pipette to add liquid multiple times in the prior art, accelerate the mixing rate, quickly glue, and mix in a closed mixing bin 3, avoiding the operator from contacting the generated toxic gas.

[0029] The bottom of the mixing pipe 2 is connected with a discharge port 4, the discharge port 4 is flat, and is a flat port capable of directly filling glue between 1.5mm glass plates, thereby omitting the process of adding liquid again after mixing.

[0030] In the description of the present application, it should be understood that the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only used to facilitate the description of the present application, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0031] The above-described embodiments are only preferred modes of the present application, and do not limit the scope of the present application. Without departing from the design spirit of the present application, various modifications and improvements to the technical solutions of the present application made by those skilled in the art shall fall within the protection scope of the present application.

Claims

1. A rapid gel preparation device for SDS-PAGE gel electrophoresis, characterized in that, include: A mixing pipe (2) and several storage pipes (1) are provided. A communication channel is fixedly installed between the bottom of the several storage pipes (1) and the mixing pipe (2). A sealing component is installed inside the storage pipe (1) to block the communication channel. A lifting component is slidably connected inside the mixing pipe (2). When the lifting component slides upward along the inner wall of the mixing pipe (2), it can penetrate the communication channel and lift the sealing component upward, so that the storage pipe (1) and the mixing pipe (2) are connected.

2. The rapid gel preparation device for SDS-PAGE gel electrophoresis according to claim 1, characterized in that: The sealing component includes a sealing ball (6) and a spring (7). One end of the spring (7) is fixedly installed on the top wall of the storage pipe (1), and the other end is fixedly connected to the sealing ball (6). The sealing ball (6) is used to seal the connecting channel.

3. The rapid gel preparation device for SDS-PAGE gel electrophoresis according to claim 2, characterized in that: The lifting component includes a slide rod (8), a connecting rod (10), and a top column (9). The two ends of the slide rod (8) are slidably connected to the inner wall of the mixing pipe (2). The connecting rod (10) is fixedly connected to the slide rod (8). The top column (9) is fixedly installed on both the slide rod (8) and the connecting rod (10). The top column (9) corresponds to the position of the sealing ball (6). The top column (9) is used to lift the sealing ball (6) upward.

4. The rapid gel preparation device for SDS-PAGE gel electrophoresis according to claim 3, characterized in that: The inner wall of the mixing tube (2) is provided with a corresponding groove (5), and the slide rod (8) slides along the groove (5).

5. The rapid gel preparation device for SDS-PAGE gel electrophoresis according to claim 4, characterized in that: Magnetic blocks (11) are fixedly installed at both ends of the slide rod (8). The magnetic blocks (11) are in contact with the slide groove (5) and slide along the slide groove (5).

6. The rapid gel preparation device for SDS-PAGE gel electrophoresis according to claim 1, characterized in that: The mixing tube (2) is provided with a discharge port (4) at the bottom, and the discharge port (4) is flat.

Images