Efficient bioreactor

Abstract

The utility model relates to an efficient bioreactor which comprises a reactor main body and a gas circulating mechanism, the gas circulation mechanism comprises a gas transmission cover, the gas transmission cover is arranged in the reactor main body, the side, provided with an opening, of the gas transmission cover is connected with the top wall in the reactor main body, and a centrifugal impeller is arranged in the gas transmission cover; two ends of the centrifugal impeller respectively extend to the lower part of the gas transmission cover and the upper part of the reactor main body, and the top end of the reactor main body is provided with a motor of which an output shaft is connected with the centrifugal impeller. Through the arrangement of the reactor main body, the gas circulating mechanism and other parts, and through the matching relationship between the motor and the centrifugal impeller, air below the communicating hole is sucked into the cover body by the centrifugal impeller, and air flow is input into a nutrient solution, so that the nutrient solution is driven to move in an air flow manner; therefore, the effect of stirring the nutrient solution is achieved.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor technology, and specifically to a high-efficiency bioreactor. Background Technology

[0002] A bioreactor is a device that enables biological reactions. In the laboratory, bioreactors are mainly used to culture biological cells. These bioreactors consist of a tank and a stirring paddle, which stirs the various nutrient solutions and biological cells inside the tank.

[0003] Traditional bioreactors use mechanical methods such as stirring paddles to generate shear forces, which directly affect the biological cells in the nutrient solution. Some sensitive biological cells are easily damaged during the stirring process. Therefore, a high-efficiency bioreactor is proposed to solve the above-mentioned problems. Utility Model Content

[0004] Based on the above description, this utility model provides a high-efficiency bioreactor to solve the problem that some sensitive cells are easily damaged during the stirring process of existing bioreactors using traditional stirring paddles.

[0005] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: a high-efficiency bioreactor, comprising: a reactor body and a gas circulation mechanism;

[0006] The gas circulation mechanism includes a gas conveying hood, which is located inside the reactor body. The side of the gas conveying hood with an opening is connected to the top wall inside the reactor body. A centrifugal impeller is installed inside the gas conveying hood, with both ends of the centrifugal impeller extending to the bottom of the gas conveying hood and the top of the reactor body, respectively. A motor with an output shaft connected to the centrifugal impeller is installed at the top of the reactor body.

[0007] Based on the above technical solution, the present invention can be further improved as follows.

[0008] Furthermore, the reactor body includes a tank with the opening facing upwards, a top cover is provided at the opening of the tank, and a motor is provided at the top of the top cover.

[0009] Furthermore, the gas conveying hood includes a hood body, which is disposed inside the tank body, and one side with an opening is connected to the lower surface of the top cover. The lower surface of the hood body is provided with a connecting hole, and the centrifugal impeller is disposed inside the hood body, extending through the connecting hole to the lower surface of the hood body.

[0010] Furthermore, the circumferential surface of the cover is provided with at least one first air supply vertical pipe, which is connected to the cover and has an air outlet at its bottom end.

[0011] Furthermore, the circumferential surface of the cover is provided with at least one second air supply vertical pipe, which is connected to the cover. The second air supply vertical pipe includes a pipe body, the bottom end of which is a closed end, and the circumferential surface of which is provided with an exhaust hole.

[0012] Furthermore, a third gas supply vertical pipe is provided on the circumferential surface of the cover, which is in contact with the inner wall of the tank and includes a gas supply main pipe. A triangular prism is sleeved on the outside of the gas supply main pipe.

[0013] Furthermore, the triangular prism has a triangular cross-section, and the side of the triangular prism facing the inner wall of the tank has an arc-shaped surface that contacts the inner wall of the tank. At least one air jet hole is provided on the side wall of the triangular prism that does not contact the tank, and the air jet hole is connected to the gas supply main pipe.

[0014] Furthermore, the centrifugal impeller includes a rotating shaft located at the output shaft of the motor, passing through the top cover and extending into the interior of the housing.

[0015] Furthermore, an impeller body is provided at the bottom end of the rotating shaft. The impeller body is located inside the cover. The air inlet of the impeller body is located directly above the connecting hole. A connecting rod is provided on the top wall inside the impeller body. The connecting rod passes through the connecting hole and extends to the bottom of the cover, and a stirring paddle is provided at its bottom end.

[0016] Furthermore, the stirring paddle includes a connector, which is connected to the connecting rod by screws and nuts, and the bottom end of the connector is provided with a transmission rod and blades from top to bottom.

[0017] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0018] 1. This utility model, by setting up components such as the reactor body and the gas circulation mechanism, and through the cooperation between the motor and the centrifugal impeller, allows the air located below the connecting hole to be drawn into the interior of the cover by the centrifugal impeller, and generates airflow that is directed into the interior of the nutrient solution. The airflow agitates the nutrient solution, so that the different parts of the solution inside the nutrient solution are mixed, thus achieving the effect of stirring the nutrient solution.

[0019] 2. By setting up the first, second, and third air supply vertical pipes, different air supply hoods can have different air supply effects. The different airflow patterns inside the nutrient solution result in different mixing effects, thus meeting different usage requirements.

[0020] 3. By setting up the stirring paddle and connecting rod, the centrifugal impeller can be connected to the stirring paddle, thus ensuring that the device can still perform mechanical stirring by means of the stirring paddle. Attached Figure Description

[0021] Figure 1 A schematic diagram of the structure of a high-efficiency bioreactor provided in an embodiment of this utility model;

[0022] Figure 2 for Figure 1 Structural sectional view;

[0023] Figure 3 for Figure 2 Another structural diagram from a different perspective;

[0024] Figure 4 This is a schematic diagram showing the structural connection between the fixed cover and the first gas delivery vertical pipe in an embodiment of this utility model;

[0025] Figure 5 This is a schematic diagram showing the structural connection between the fixed cover and the second air supply vertical pipe in an embodiment of this utility model;

[0026] Figure 6 This is a schematic diagram showing the structural connection between the fixed cover and the third gas delivery vertical pipe in an embodiment of this utility model;

[0027] Figure 7 This is a schematic diagram of the centrifugal impeller in an embodiment of the present invention;

[0028] Figure 8 for Figure 7 Another structural diagram from a different perspective;

[0029] Figure 9 This is a schematic diagram of the structure of the stirring paddle in an embodiment of this utility model;

[0030] The attached diagram lists the components represented by each number as follows:

[0031] 1. Tank body; 2. Top cover; 3. Motor; 4. Gas supply hood; 41. Hood body; 42. Connecting hole; 43. First gas supply vertical pipe; 44. Second gas supply vertical pipe; 441. Pipe body; 442. Exhaust port; 45. Third gas supply vertical pipe; 451. Main gas supply pipe; 452. Triangular prism; 453. Jet nozzle; 5. Centrifugal impeller; 51. Rotating shaft; 52. Impeller body; 53. Connecting rod; 6. Agitator; 61. Connecting piece; 62. Transmission rod; 63. Blade. Detailed Implementation

[0032] To facilitate understanding of this application, a more complete description will be provided below with reference to the accompanying drawings, which illustrate embodiments of the present application. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of this application will be thorough and complete.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0034] When used herein, the singular forms of “a,” “an,” and “the” may also include the plural forms unless the context clearly indicates otherwise. It should also be understood that the terms “comprising,” “including,” or “having,” etc., specify the presence of the stated feature, whole, step, operation, component, part, or combination thereof, but do not preclude the possibility of the presence or addition of one or more other features, wholes, steps, operations, components, parts, or combinations thereof.

[0035] Example 1

[0036] Please see Figures 1-3 A high-efficiency bioreactor, comprising: a reactor body and a gas circulation mechanism;

[0037] The reactor body includes a tank 1 with the opening facing upwards, a top cover 2 is provided at the opening of the tank 1, and a motor 3 is provided at the top of the top cover 2;

[0038] Based on the above, the main body of the reactor is an existing high-efficiency bioreactor, in which the tank 1, the top cover 2 and the motor 3 are all parts of the existing technology.

[0039] like Figures 2-4 As shown, the gas circulation mechanism includes a gas conveying hood 4, which is disposed inside the reactor body. The side of the gas conveying hood 4 with an opening is connected to the top wall inside the reactor body. A centrifugal impeller 5 is disposed inside the gas conveying hood 4. The two ends of the centrifugal impeller 5 extend to the bottom of the gas conveying hood 4 and the top of the reactor body, respectively. A motor 3 with an output shaft connected to the centrifugal impeller 5 is disposed at the top of the reactor body.

[0040] The gas supply hood 4 includes a hood body 41, which is disposed inside the tank body 1 and has an opening on one side connected to the lower surface of the top cover 2. The lower surface of the hood body 41 is provided with a connecting hole 42. The centrifugal impeller 5 is disposed inside the hood body 41 and extends through the connecting hole 42 to the lower surface of the hood body 41. At least one first gas supply vertical pipe 43 is provided on the circumferential surface of the hood body 41. The first gas supply vertical pipe 43 is interconnected with the hood body 41 and has a gas outlet at its bottom end.

[0041] Based on the above, the cover 41 provides sufficient space for the rotation of the centrifugal impeller 5, and the connection hole 42 allows the centrifugal impeller 5 to draw air from outside the cover 41 into the interior of the cover 41 during rotation. Under the action of the centrifugal impeller 5, the air is thrown out from the side. The thrown air forms an airflow and enters the nutrient solution from the air outlet at the bottom of the first air supply vertical pipe 43. The air flowing into the nutrient solution agitates the nutrient solution, thereby enabling the various components inside the nutrient solution to mix, thus achieving the stirring effect.

[0042] like Figure 2 , Figure 3 , Figure 7 and Figure 8 As shown, the bottom end of the rotating shaft 51 is provided with an impeller body 52, which is located inside the cover 41. The air inlet of the impeller body 52 is located directly above the connecting hole 42. The top wall inside the impeller body 52 is provided with a connecting rod 53, which extends through the connecting hole 42 to the bottom of the cover 41, and a stirring paddle 6 is provided at its bottom end.

[0043] Based on the above, the centrifugal impeller 5, driven by the motor 3, cooperates with the cover 41 to form a centrifugal fan. When the centrifugal impeller 5 rotates, it draws air from outside the cover 41 into the interior of the cover 41 through the connecting hole 42, and under the action of the centrifugal impeller 5, the airflow is output from the first air delivery vertical pipe 43.

[0044] like Figure 9 As shown, the stirring paddle 6 includes a connecting member 61, which is connected to the connecting rod 53 by screws and nuts. The bottom end of the connecting member 61 is provided with a transmission rod 62 and a blade 63 from top to bottom.

[0045] Based on the above, the stirring paddle 6 serves to stir the nutrient solution. The installation and use of the stirring paddle 6 can be selected according to the actual situation. When the reactor is used for less sensitive biological cells, the stirring paddle 6 can be used directly for stirring. If the biological cells are sensitive, the stirring paddle 6 can be disassembled to avoid mechanical stirring from damaging the biological cells.

[0046] Example 2

[0047] Based on Example 1, such as Figure 5 As shown, the circumferential surface of the cover 41 is provided with at least one second air supply vertical pipe 44. The second air supply vertical pipe 44 is connected to the cover 41. The second air supply vertical pipe 44 includes a pipe body 441. The bottom end of the pipe body 441 is a closed end. The circumferential surface of the pipe body 441 is provided with an exhaust hole 442.

[0048] Based on the above, the second air supply vertical pipe 44 allows the air supply hood 4 to input airflow into the nutrient solution in another way. Compared with the first air supply vertical pipe 43 directly inputting airflow into the nutrient solution, the second air supply vertical pipe 44, through the exhaust hole 442 opened on the circumferential surface of the pipe body 441, allows the airflow to enter the nutrient solution from the side when it is transported along the pipe body 441, so that the airflow can fully agitate the nutrient solution.

[0049] Example 3

[0050] Based on Example 1, such as Figure 6 As shown, a third air supply vertical pipe 45 is provided on the circumferential surface of the cover 41, which is in contact with the inner wall of the tank 1 and includes an air supply main pipe 451. A triangular prism 452 is sleeved on the outside of the air supply main pipe 451. The cross-section of the triangular prism 452 is triangular, and the side of the triangular prism 452 facing the inner wall of the tank 1 is an arc-shaped surface. The arc-shaped surface is in contact with the inner wall of the tank 1. At least one jet hole 453 is provided on the side wall of the triangular prism 452 that is not in contact with the tank 1. The jet hole 453 is in communication with the air supply main pipe 451.

[0051] Based on the above, the setting of the third gas supply vertical pipe 45 allows the airflow inside the gas supply hood 4 to be input into the nutrient solution in another way. After the airflow flows through the gas supply main pipe 451, it is ejected from the jet hole 453. The ejected airflow moves along the direction of the inner wall of the tank 1, that is, the airflow is an annular airflow. The annular airflow can drive the nutrient solution to move along the direction of the inner wall of the tank 1, thereby achieving the effect of mixing the components of the nutrient solution, that is, achieving the effect of shaftless stirring.

[0052] In the above embodiments, the air inside the tank 1 is circulated through a gas circulation mechanism. That is, the air above the nutrient solution is input into the nutrient solution under the action of the gas circulation mechanism and then returns to the top of the nutrient solution. During this process, the centrifugal impeller 5 and the cover 41 are both located above the nutrient solution, while the first gas supply pipe 43, the second gas supply pipe 44 and the third gas supply pipe 45 extend into the nutrient solution, so that the airflow inside the cover 41 can be input into the nutrient solution through the above three gas supply pipes.

[0053] This allows airflow to replace mechanical stirring, and compared to the shear force brought by mechanical stirring, airflow makes it easier for biological cells to be preserved intact in the nutrient solution.

[0054] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A high efficiency bioreactor characterized in that, The utility model relates to a reactor, including: a reactor body and a gas circulating mechanism; the gas circulating mechanism includes a gas distribution cover (4) arranged inside the reactor body, the gas distribution cover (4) is provided with the side of the opening and is connected with the top wall inside the reactor body, the inside of the gas distribution cover (4) is provided with a centrifugal impeller (5), both ends of the centrifugal impeller (5) extend to below the gas distribution cover (4) and above the reactor body respectively, and the top end of the reactor body is provided with a motor (3) connected with the centrifugal impeller (5).

2. The high efficiency bioreactor of claim 1, wherein, The reactor body includes a tank body (1) with the tank mouth upward, the tank mouth of the tank body (1) is provided with a top cover (2), and the top end of the top cover (2) is provided with a motor (3).

3. The high efficiency bioreactor of claim 2, wherein, The gas distribution cover (4) includes a cover body (41) arranged inside the tank body (1) and provided with the side of the opening and connected with the lower surface of the top cover (2), the lower surface of the cover body (41) is provided with a communication hole (42), the centrifugal impeller (5) is arranged inside the cover body (41) and extends to the lower surface of the cover body (41) after penetrating the communication hole (42).

4. The high efficiency bioreactor of claim 3, wherein, The circumferential surface of the cover body (41) is provided with at least one first gas distribution vertical pipe (43) in communication with the cover body (41) and provided with a gas outlet at the bottom end.

5. The high efficiency bioreactor of claim 3, wherein, The circumferential surface of the cover body (41) is provided with at least one second gas distribution vertical pipe (44) in communication with the cover body (41), and the second gas distribution vertical pipe (44) includes a pipe body (441) with a closed bottom end, and the circumferential surface of the pipe body (441) is provided with an exhaust hole (442).

6. The high efficiency bioreactor of claim 3, wherein, The circumferential surface of the cover body (41) is provided with a third gas distribution vertical pipe (45) in communication with the inner wall of the tank body (1) and including a gas distribution main pipe (451), and the outer part of the gas distribution main pipe (451) is sleeved with a triangular prism (452).

7. The high efficiency bioreactor of claim 6, wherein, The cross section of the triangular prism (452) is triangular, one side of the triangular prism (452) facing the inner wall of the tank body (1) is an arc surface, the arc surface is in contact with the inner wall of the tank body (1), at least one jet hole (453) is arranged on the side wall of the side of the triangular prism (452) not in contact with the tank body (1), and the jet hole (453) is in communication with the gas distribution main pipe (451).

8. The high efficiency bioreactor of claim 3, wherein, The centrifugal impeller (5) includes a rotating shaft (51) arranged at the output shaft of the motor (3) and extending to the inside of the cover body (41) after penetrating the top cover (2).

9. The high efficiency bioreactor of claim 8, wherein, The bottom end of the rotating shaft (51) is provided with an impeller body (52), the impeller body (52) is arranged in the inside of the cover body (41), the air inlet of the impeller body (52) is arranged directly above the communication hole (42), the top wall in the inside of the impeller body (52) is provided with a connecting rod (53), the connecting rod (53) extends to the below of the cover body (41) after penetrating the communication hole (42), and the bottom end is provided with a stirring paddle (6).

10. The high efficiency bioreactor of claim 9, wherein, The stirring paddle (6) comprises a connecting piece (61), the connecting piece (61) is connected with the connecting rod (53) through screws and nuts, and the bottom end of the connecting piece (61) is sequentially provided with a transmission rod (62) and a blade (63) from top to bottom.

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