Skin fibroblast culture bottle

Abstract

The utility model discloses a skin fibroblast culture bottle which comprises a bottle body, a bottle neck is integrally formed at one end of the bottle body, a ventilation structure is in threaded connection with the outer side of one end of the bottle neck, a clamping seat is integrally formed at the top end of the bottle body, a clamping ring is integrally formed at the bottom end of the bottle body, and a culture structure is arranged on the outer side of the bottle body. A marking structure is installed on one side of the bottle body. According to the cell culture bottle, the rotatable ventilation valve is mounted at one end of the bottle cap, and the bottle cap and the ventilation valve are provided with the ventilation holes, so that air inside and outside the cell culture bottle can be exchanged when the ventilation holes in the bottle cap and the ventilation valve are overlapped, and air exchange can be stopped when the ventilation holes in the bottle cap and the ventilation valve are staggered by rotating the ventilation valve; and the polytetrafluoroethylene filter membrane in the bottle cap can ensure the entry of carbon dioxide and the discharge of oxygen and prevent microorganisms and pollutants from entering, so that the aim that the skin fibroblast culture bottle can ensure the air permeability of a growth environment is fulfilled.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a skin fibroblast culture flask. Background Technology

[0002] Skin fibroblasts, also known as fibroblasts, are the main cellular components of the dermis in human skin. They differentiate from mesenchymal cells during the embryonic period and are the main cells of loose connective tissue, accounting for more than 95% of all cells in the dermis. They are important cells for maintaining skin structure and function. Fibroblasts are spindle-shaped or flattened star-shaped with processes. The cells and nuclei are relatively large and have clear outlines. The nuclei are regularly oval and large and prominent. Fibroblasts can synthesize and secrete matrix components such as collagen, elastin, glycosaminoglycans, and glycoproteins. These matrix components are crucial for maintaining skin firmness, elasticity, and hydration. After epidermal damage, surgical incisions, chronic inflammation, infection, or tissue ischemia, fibroblasts become active to help repair damaged tissue. They promote wound healing by proliferating, migrating to the wound site, and secreting extracellular matrix to remodel the wound bed. Cell culture flasks are a commonly used instrument in biological experiments, mainly used for cell culture, expansion, and preservation. They are special containers, usually made of glass or plastic, with different bottom shapes and volumes to meet different experimental needs.

[0003] To address this, Chinese patent application CN207227449U discloses a cell culture flask, comprising a flask body and a cap. The flask body has a mouth, which includes a large cylindrical body and a small cylindrical body arranged coaxially. The large cylindrical body is connected to the flask body, and the small cylindrical body is made of tempered glass. The cap includes a first threaded section that is threadedly connected to the large cylindrical body and a second threaded section that is threadedly connected to the small cylindrical body. This structure of the cell culture flask ensures airtightness and prevents cell contamination during culture.

[0004] Current skin fibroblast culture flasks can basically meet people's needs, but some problems still exist, as detailed below:

[0005] 1. Skin fibroblast culture flasks have difficulty ensuring the permeability of the growth environment. During cell culture, cells need to carry out normal metabolic activities, including oxygen intake and carbon dioxide expulsion, to ensure that cells can obtain sufficient oxygen and expel the carbon dioxide produced by metabolism. A completely sealed culture environment will affect cell growth and metabolism.

[0006] 2. The problem of accurate and clear labeling of skin fibroblast culture flasks: During cell culture, each culture flask needs to be clearly labeled to distinguish cells from different batches, different treatment conditions or different experimental groups. However, cell culture flasks are all the same shape, which can easily cause confusion, thereby interfering with the culture structure and affecting the final data results. Utility Model Content

[0007] The purpose of this invention is to provide a skin fibroblast culture flask to solve the shortcomings of existing skin fibroblast culture flasks, which are difficult to guarantee the permeability of the growth environment and are difficult to accurately and clearly label.

[0008] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a skin fibroblast culture flask, including a flask body;

[0009] One end of the bottle body is integrally formed with a bottleneck, and the outer side of the bottleneck end is threaded with a breathable structure.

[0010] The breathable structure includes a bottle cap, a breathable valve, a breathable hole, a polytetrafluoroethylene (PTFE) filter membrane, a fixing ring, and anti-slip texture. The bottle cap is threaded to the outside of one end of the bottle neck. The breathable valve is rotatably connected to one end of the bottle cap. A PTFE filter membrane is installed inside the bottle cap. A fixing ring is installed at one end of the PTFE filter membrane.

[0011] The top of the bottle body is integrally formed with a retaining seat, the bottom of the bottle body is integrally formed with a retaining ring, a culture structure is provided on the outer side of the bottle body, and a marking structure is installed on one side of the bottle body.

[0012] When using, first ensure the cell culture flask is in a sterile environment. Twist the cap to remove it from the neck of the flask, place the flask upright, and use a pipette to inject the culture medium into the flask. You can determine the volume by checking the volume markings on the side of the flask. Then, screw the cap back onto the neck of the flask. Next, lay the cell culture flask flat with the frosted side facing down. Gently shake the flask to spread the culture medium evenly on the frosted surface. Adjust the vent valve according to the culture requirements to ensure the cell culture flask is breathable. Multiple cell culture flasks can be stacked by inserting the retaining ring into the retaining seat. Finally, place them all in the cell culture incubator.

[0013] Furthermore, one end of the bottle cap is provided with a sliding groove, and one end of the vent valve is provided with a slider. The bottle cap and the vent valve are slidably connected, which facilitates the rotation of the vent valve to adjust the air permeability of the cell culture flask.

[0014] Furthermore, the vent valve has vent holes inside, which are arranged in a ring shape, allowing the vent holes of the vent valve and the bottle cap to overlap or stagger each other.

[0015] Furthermore, a locking block is provided on the outer side of the fixing ring, and a locking groove is provided on the inner side of the bottle cap. The fixing ring and the bottle cap are connected by a locking mechanism to fix the polytetrafluoroethylene filter membrane, and the polytetrafluoroethylene filter membrane can also be replaced when it is damaged.

[0016] Furthermore, the bottle cap and the vent valve are both provided with anti-slip textures on their outer sides. These anti-slip textures are arranged at equal intervals on the outer sides of the bottle cap and the vent valve to increase friction and facilitate the opening and closing of the cell culture bottle and the adjustment of air permeability.

[0017] Furthermore, the culture structure includes volume markings, length markings, and a frosted layer. The volume markings are located on one side of the bottle, the length markings are located on one side and one end of the bottle, and the frosted layer is located at the bottom of the inner side of the bottle, which facilitates internal observation and cell growth.

[0018] Furthermore, the marking structure includes a label groove, a recess, and a label plate. The label groove is adhered to one side of the bottle body, and a recess is provided at the top of the label groove. The label plate is movably installed on the inner side of the label groove, making it easier for users to write on it and protect the marking.

[0019] This utility model provides a skin fibroblast culture flask with the following advantages: A rotatable vent valve is installed at one end of the cap. Both the cap and the vent valve have vent holes. When the vent holes on the cap and the vent valve overlap, gas exchange between the inside and outside of the cell culture flask is allowed. When the vent valve is rotated to separate the vent holes, gas exchange is prevented. The polytetrafluoroethylene (PTFE) filter membrane inside the cap ensures the entry of carbon dioxide and the exit of oxygen, while preventing the entry of microorganisms and contaminants. The PTFE filter membrane is fixed by a retaining ring. Anti-slip textures are provided on the outer sides of both the cap and the vent valve to facilitate opening and closing of the cell culture flask and adjusting permeability. This achieves the goal of ensuring the permeability of the growth environment for skin fibroblast culture flasks.

[0020] By installing a label slot on one side of the bottle, a label plate can be placed inside. The surface of the label plate can be repeatedly written on, making it easy for users to label cell culture flasks and avoid mixing up multiple cell culture flasks, which would affect the final data. The label slot is completely transparent, which can protect the label plate without affecting observation. The top of the label slot has a groove for easy removal and placement of the label plate. The label slot is installed on the side of the bottle, ensuring the integrity of the observation surface at the top of the cell culture flask. This achieves the goal of accurate and clear labeling of skin fibroblast culture flasks. Attached Figure Description

[0021] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0022] Figure 2This is a side view of the structure of this utility model;

[0023] Figure 3 This is a frontal cross-sectional view of the present invention.

[0024] Figure 4 This is a three-dimensional exploded view of the air-permeable structure of this utility model;

[0025] Figure 5 This is a partial cross-sectional view of the breathable structure of this utility model.

[0026] The following are the labels in the attached diagram: 1. Bottle body; 2. Bottle neck; 3. Ventilation structure; 301. Bottle cap; 302. Ventilation valve; 303. Ventilation hole; 304. PTFE filter membrane; 305. Fixing ring; 306. Anti-slip texture; 4. Card holder; 5. Card ring; 6. Culture structure; 601. Volume scale; 602. Length scale; 603. Frosted layer; 7. Marking structure; 701. Label groove; 702. Groove; 703. Label plate. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] Please see Figures 1-5 One embodiment of this utility model is a skin fibroblast culture flask, comprising a flask body 1.

[0029] One end of the bottle body 1 has a neck 2 integrally formed. A venting structure 3 is threadedly connected to the outer side of one end of the neck 2. The venting structure 3 includes a bottle cap 301, a vent valve 302, a vent hole 303, a polytetrafluoroethylene filter membrane 304, a fixing ring 305, and anti-slip texture 306. The bottle cap 301 is threadedly connected to the outer side of one end of the neck 2. A vent valve 302 is rotatably connected to one end of the bottle cap 301. One end of the bottle cap 301 has a sliding groove, and one end of the vent valve 302 has a slider. The bottle cap 301 and the vent valve 302 are slidably connected. The bottle cap 302 has a vent 303 inside, which is arranged in a ring. The bottle cap 301 has a polytetrafluoroethylene filter membrane 304 installed inside. A fixing ring 305 is installed at one end of the polytetrafluoroethylene filter membrane 304. A locking block is provided on the outside of the fixing ring 305. A locking groove is provided on the inside of the bottle cap 301. The fixing ring 305 and the bottle cap 301 are locked together. Anti-slip textures 306 are provided on the outside of both the bottle cap 301 and the vent valve 302. The anti-slip textures 306 are arranged at equal intervals on the outside of the bottle cap 301 and the vent valve 302.

[0030] See attached document Figure 1-2 and attached Figure 4-5 As shown, a rotatable vent valve 302 is installed at one end of the bottle cap 301. Both the bottle cap 301 and the vent valve 302 have vent holes 303. When the vent holes 303 on the bottle cap 301 and the vent valve 302 overlap, the gas inside and outside the cell culture bottle can be exchanged. When the vent valve 302 is rotated to offset the vent holes 303, the gas exchange can be prevented. The polytetrafluoroethylene filter membrane 304 inside the bottle cap 301 can ensure the entry of carbon dioxide and the exit of oxygen, while preventing the entry of microorganisms and contaminants. The polytetrafluoroethylene filter membrane 304 is fixed by a fixing ring 305. The outer sides of the bottle cap 301 and the vent valve 302 are provided with anti-slip textures 306 to facilitate the opening and closing of the cell culture bottle and the adjustment of the permeability.

[0031] The top of the bottle body 1 has a one-piece molded seat 4, and the bottom of the bottle body 1 has a one-piece molded retaining ring 5.

[0032] A culture structure 6 is provided on the outside of the bottle body 1. The culture structure 6 includes a volume scale 601, a length scale 602 and a frosted layer 603. The volume scale 601 is provided on one side of the bottle body 1, the length scale 602 is provided on one side and one end of the bottle body 1, and the frosted layer 603 is provided at the bottom of the inner side of the bottle body 1.

[0033] See attached document Figure 1-3As shown, a volume scale 601 is provided on one side of the bottle body 1, and a length scale 602 is provided on one end and one side. Both the volume scale 601 and the length scale 602 are embossed to avoid the problem of the scale falling off during operation. At the same time, it allows users to more intuitively observe the changes inside the bottle body 1 and accurately measure and add culture medium or other solutions. The frosted layer 603 provided at the bottom of the inner side of the bottle body 1 can increase the contact area between the cells and the bottom of the bottle body 1, making it easier for the cells to find attachment points, which is conducive to the cell adhesion and growth.

[0034] A marking structure 7 is installed on one side of the bottle body 1. The marking structure 7 includes a label groove 701, a recess 702 and a label plate 703. The label groove 701 is adhered to one side of the bottle body 1. A recess 702 is provided at the top of the label groove 701. A label plate 703 is movably installed on the inner side of the label groove 701.

[0035] See attached document Figure 1-2 As shown, a label slot 701 is installed on one side of the bottle body 1. A label plate 703 can be placed in the label slot 701. The surface of the label plate 703 can be repeatedly written on, which makes it convenient for users to label the cell culture flasks and avoid mixing up multiple cell culture flasks, thus affecting the final data. The label slot 701 is completely transparent, which can protect the label plate 703 without affecting observation. A groove 702 is provided at the top of the label slot 701 to facilitate the picking up and placing of the label plate 703. The label slot 701 is installed on the side of the bottle body 1 to ensure the integrity of the observation surface at the top of the cell culture flask.

[0036] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A skin fibroblast cell culture bottle, comprising a bottle body (1); Characterized in that: One end of the bottle body (1) is integrally formed with a bottle neck (2), and the outer side of one end of the bottle neck (2) is threadedly connected with a breathable structure (3); The breathable structure (3) comprises a bottle cap (301), a breathable valve (302), a breathable hole (303), a polytetrafluoroethylene filter membrane (304), a fixing ring (305) and an anti-skid pattern (306), the bottle cap (301) is threadedly connected to the outer side of one end of the bottle neck (2), one end of the bottle cap (301) is rotatably connected with the breathable valve (302), the inside of the bottle cap (301) is provided with the polytetrafluoroethylene filter membrane (304), and one end of the polytetrafluoroethylene filter membrane (304) is provided with the fixing ring (305); The top end of the bottle body (1) is integrally formed with a clamping seat (4), the bottom end of the bottle body (1) is integrally formed with a clamping ring (5), the outer side of the bottle body (1) is provided with a culture structure (6), and one side of the bottle body (1) is provided with a marking structure (7).

2. The skin fibroblast cell culture flask according to claim 1, wherein: One end of the bottle cap (301) is provided with a sliding groove, one end of the breathable valve (302) is provided with a sliding block, and the bottle cap (301) and the breathable valve (302) are slidably connected.

3. The skin fibroblast cell culture flask according to claim 1, wherein: The inside of the breathable valve (302) is provided with a breathable hole (303), and the breathable hole (303) is annularly distributed.

4. The skin fibroblast cell culture flask according to claim 1, wherein: The outer side of the fixing ring (305) is provided with a clamping block, the inner side of the bottle cap (301) is provided with a clamping groove, and the fixing ring (305) and the bottle cap (301) are clampedly connected.

5. The skin fibroblast cell culture flask according to claim 1, wherein: The outer sides of the bottle cap (301) and the breathable valve (302) are provided with anti-skid patterns (306), and the anti-skid patterns (306) are arranged at equal intervals on the outer sides of the bottle cap (301) and the breathable valve (302).

6. The skin fibroblast culture flask according to claim 1, wherein: The culture structure (6) comprises a volume scale (601), a length scale (602) and a frosted layer (603), the volume scale (601) is arranged on one side of the bottle body (1), the length scale (602) is arranged on one side and one end of the bottle body (1), and the frosted layer (603) is arranged at the bottom end of the inner side of the bottle body (1).

7. The skin fibroblast culture flask according to claim 1, wherein: The marking structure (7) comprises a label slot (701), a groove (702) and a label plate (703), the label slot (701) is adhered to one side of the bottle body (1), the top end of the label slot (701) is provided with the groove (702), and the inner side of the label slot (701) movably installs the label plate (703).

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