Sterile cell culture pore plate capable of being penetrated by ultraviolet rays

Abstract

The utility model relates to the technical field of medical apparatus and instruments, in particular to an ultraviolet-penetrable sterile cell culture pore plate which comprises a quartz seat, an ultraviolet light source, an ultraviolet light source, an ultraviolet light source and an ultraviolet light source, the partition plate is of a cylindrical structure and is arranged in the cavity, and at least one partition plate is arranged in the cavity; the quartz cover is arranged above the partition plate and can cover the partition plate; and the ultraviolet lamp assembly is arranged above the quartz cover or below the quartz seat. The ultraviolet lamp assembly is arranged above the quartz cover or below the quartz base, so that the distance between the ultraviolet lamp assembly and cells in the partition plate is fixed, and the quartz base and the quartz cover are arranged, so that ultraviolet waves can penetrate conveniently, the quartz cover does not need to be opened repeatedly in the cell culture process, and the cell culture efficiency is improved. The risk of cell pollution is greatly reduced, evaporation and flowing of water are guaranteed, and a better guarantee is provided for a stimulation in-vitro experiment of cells.

Description

Technical Field

[0001] This utility model belongs to the field of medical device technology and relates to a sterile cell culture plate that is permeable to ultraviolet light. Background Technology

[0002] In in vitro experiments studying the effects of ultraviolet (UV) radiation on cells, researchers typically use culture dishes / plates of varying pore sizes to seed suspension / adherent cells. Since UV waves cannot penetrate plastic or colored liquids, once the cells are stable, the culture lid is first opened in a biosafety cabinet, and the colored culture medium is removed. The cells are then rinsed several times with PBS buffer, leaving a small amount of liquid to prevent dehydration. Most researchers use UV lamps, placed at a certain distance from the cells. The irradiation dose (1J = 1Ws) is calculated based on the lamp's power, allowing them to determine the energy and intensity of UV radiation received by the cells. Experimental and control groups are set up, and changes in cell behavior patterns (cycle, apoptosis, proliferation) and the expression levels of DNA damage-related molecules are analyzed to evaluate photoaging / damage / malignant transformation caused by UV radiation. Therefore, UV irradiation is particularly important for in vitro cell studies.

[0003] The available ultraviolet irradiation equipment varies considerably. Some teams use clinical treatment equipment, such as narrow-band UVB for patients with psoriasis and vitiligo; others simply use ultraviolet lamps purchased online. These devices have variable wavelengths and power, resulting in significant differences in calculated radiation doses. Due to substantial human error in each operation, the experimental results are poorly reproducible, and the intensity of radiation stimulation received by cells in the central and peripheral regions is uneven, with adjacent holes easily influencing each other.

[0004] The distance between the ultraviolet light source and the cells is difficult to control, and measuring the distance before each treatment is cumbersome and difficult to standardize. If irradiation is performed from the bottom of the well plate, the ultraviolet waves cannot pass through due to the barrier material of the plate. Repeatedly opening the cell culture lid causes the cells to deviate from the optimal growth temperature and air environment, which is itself an adverse stimulus and increases the instability of experimental results. At the same time, the irradiation process increases the risk of cell contamination and causes culture medium evaporation and water loss.

[0005] Therefore, this invention proposes a sterile cell culture plate that is permeable to ultraviolet light. Utility Model Content

[0006] This utility model provides a sterile cell culture plate that is permeable to ultraviolet light, comprising: a quartz base 1 with an internal cavity; a partition 2, which is a cylindrical structure and is disposed in the cavity, with at least one partition 2 in the cavity; a quartz cover 3, disposed above the partition 2 and capable of covering the partition 2; and an ultraviolet lamp assembly 5, disposed above the quartz cover 3 or below the quartz base 1.

[0007] Furthermore, a polymethyl methacrylate nanocoating 6 is applied to the upper surface of the quartz base 1 and within the area enclosed by the partition 2.

[0008] Furthermore, a titanium dioxide coating 7 is applied to the outer wall of the partition 2.

[0009] Furthermore, a limiting ring 4 is provided on the side of the quartz cover 3 facing the partition 2, and the limiting ring 4 cooperates with the partition 2.

[0010] Furthermore, the number of partitions 2 is 6.

[0011] Furthermore, six partitions 2 are evenly spaced on the quartz base 1.

[0012] Furthermore, the ultraviolet lamp assembly 5 includes: a lamp carrier plate 51, which is a flat plate structure; an ultraviolet bulb 52, which is disposed on the lamp carrier plate 51, and the position of the ultraviolet bulb 52 corresponds to the position of the partition plate 2; and a light shield 53, which is disposed on the periphery of the lamp carrier plate 51 and is detachably connected to the lamp carrier plate 51.

[0013] Furthermore, each partition 2 has 9 light mounting holes.

[0014] Furthermore, the nine lamp mounting holes are arranged such that there are three lamp mounting holes in each row and three lamp mounting holes in each column, with the same distance between two adjacent lamp mounting holes in each row or column.

[0015] Furthermore, the ultraviolet lamp assembly 5 includes a battery 8 disposed below the lamp carrier plate 51 and electrically connected to the ultraviolet bulb 52.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows.

[0017] 1. Quartz glass has high biocompatibility, chemical stability, and low biotoxicity. Furthermore, ultraviolet light can pass through quartz glass; therefore, the cell culture cap is changed to a UV-transmitting quartz material, while the material around the cap remains unchanged. Before each irradiation, the colored culture medium is blotted dry and washed with PBS buffer as usual. During irradiation, the cap is closed, and the machine irradiates from above the cap to avoid the risk of contamination and gas temperature imbalance caused by opening the cap.

[0018] 2. Replacing the plastic bottom of the culture plate with quartz glass can be problematic because quartz glass has both hydrophilic and hydrophobic properties, which can negatively impact cell growth and hinder proper cell adhesion. If the cultured cells are adherent, adding a polymethyl methacrylate (PMMA) nanoparticle film facilitates cell adhesion without affecting UV transmission. During UV irradiation, the cell plate is placed on a UV device, allowing UV light to directly irradiate the cells from the bottom. For adherent cells, direct UV stimulation eliminates the need for medium changes and buffer washing. Avoiding frequent medium changes reduces the impact of repeated alterations in secreted proteins or culture medium composition on the cells. It also minimizes carbon dioxide loss and temperature drops during opening.

[0019] 3. Both the bottom and the cover of the perforated plate are made of quartz material, with PMMA nanoparticles embedded in the bottom. The outer sidewall of the perforated plate is covered with a thin layer of titanium dioxide, which can absorb ultraviolet light waves, ensuring that each hole can only receive light from that location and avoid interference from neighboring light sources. Due to its photocatalytic properties, it can generate highly oxidizing active substances during ultraviolet irradiation, reducing the possibility of pollution and meeting the irradiation needs of different scenarios. Attached Figure Description

[0020] Figure 1 A schematic diagram of the overall perforated plate structure of this utility model.

[0021] Figure 2 A schematic diagram of the reverse side structure of the cover plate of this utility model.

[0022] Figure 3 A schematic diagram of the assembly of the partition and the quartz base of this utility model.

[0023] Figure 4 A schematic diagram of the structure of the ultraviolet lamp assembly of this utility model.

[0024] Figure 5 A schematic diagram of the internal structure of the ultraviolet lamp assembly of this utility model.

[0025] Figure 6 A schematic diagram of the structure of the ultraviolet lamp assembly of this utility model, which is set above the quartz cover.

[0026] Figure 7 A schematic diagram of the structure of the ultraviolet lamp assembly of this utility model, which is located below the quartz base.

[0027] Reference numerals: 1. Quartz base; 2. Divider; 3. Quartz cover; 4. Limiting ring; 5. UV lamp assembly; 51. Lamp carrier plate; 52. UV bulb; 53. Light shield; 6. Polymethyl methacrylate nano-coating; 7. Titanium dioxide coating; 8. Battery. Detailed Implementation

[0028] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this invention. Therefore, the drawings and description are considered exemplary in nature and not restrictive.

[0029] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0030] like Figures 1-7 As shown, this utility model embodiment provides an ultraviolet-permeable sterile cell culture plate, comprising: a quartz base 1 with an internal cavity; a partition 2, which is a cylindrical structure disposed within the cavity, with at least one partition 2 within the cavity; a quartz cover 3 disposed above the partition 2 and capable of covering the partition 2; and an ultraviolet lamp assembly 5 disposed above the quartz cover 3 or below the quartz base 1; the partition and the quartz base are glued together; the entire structure of the cell culture plate is transparent; and the cover and base are made of quartz material, solving the problem that ultraviolet waves cannot pass through traditional plastic materials, requiring repeated opening and closing of the cover for stimulation.

[0031] In one embodiment, a polymethyl methacrylate (PMMA) nanocoating 6 is applied to the upper surface of the quartz base 1, within the area enclosed by the partition 2. The PMMA nanocoating facilitates cell adhesion without affecting ultraviolet light transmission.

[0032] In one embodiment, a titanium dioxide coating 7 is applied to the outer wall of the partition 2. The titanium dioxide coating can absorb ultraviolet light waves, ensuring that a hole can only receive light from that location and avoid interference from neighboring light sources. Furthermore, due to its photocatalytic properties, it can generate highly oxidizing active substances during ultraviolet irradiation, reducing the possibility of pollution and meeting the irradiation needs of different scenarios.

[0033] In one embodiment, a limiting ring 4 is provided on the side of the quartz cover 3 facing the partition 2, and the limiting ring 4 cooperates with the partition 2. The cooperation between the limiting ring and the partition allows the quartz cover to be completely closed with the partition, meeting the requirements of a sterile environment.

[0034] In one embodiment, there are six partitions 2; the six partitions 2 are evenly spaced on the quartz base 1. Multiple cell experiments can be performed within the same cell culture plate.

[0035] In one embodiment, the ultraviolet lamp assembly 5 includes: a lamp carrier plate 51, which is a flat plate structure; an ultraviolet bulb 52, disposed on the lamp carrier plate 51, the position of the ultraviolet bulb 52 corresponding to the position of the partition plate 2; and a light-shielding plate 53, disposed on the periphery of the lamp carrier plate 51, and detachably connected to the lamp carrier plate 51. Each partition plate 2 has nine lamp mounting holes; the nine lamp mounting holes are arranged in a row of three and a column of three, with the distance between any two adjacent lamp mounting holes in each row or column being the same.

[0036] In one embodiment, the ultraviolet lamp assembly 5 includes a battery 8 disposed below the lamp carrier plate 51 and electrically connected to the ultraviolet bulb 52; the battery may be an externally connected wire type or a rechargeable type.

Claims

1. A sterile cell culture plate that is permeable to ultraviolet light, characterized in that, include: Quartz base (1), with an internal cavity; The partition (2) is a cylindrical structure and is disposed in the cavity, and there is at least one partition (2) in the cavity. A quartz cover (3) is disposed above the partition (2) and can be closed with the partition (2); The ultraviolet lamp assembly (5) is located above the quartz cover (3) or below the quartz base (1).

2. The ultraviolet-permeable sterile cell culture plate according to claim 1, characterized in that: On the upper surface of the quartz base (1) and within the area enclosed by the partition plate (2), a polymethyl methacrylate nanocoating (6) is applied.

3. The ultraviolet-permeable sterile cell culture plate according to claim 1, characterized in that: The outer wall of the partition (2) is covered with a titanium dioxide coating (7).

4. The ultraviolet-permeable sterile cell culture plate according to claim 1, characterized in that: The quartz cover (3) has a limiting ring (4) on the side facing the partition (2), and the limiting ring (4) cooperates with the partition (2).

5. The ultraviolet-permeable sterile cell culture plate according to claim 1, characterized in that: The number of partitions (2) is 6.

6. The ultraviolet-permeable sterile cell culture plate according to claim 5, characterized in that: The six partitions (2) are evenly spaced on the quartz base (1).

7. The ultraviolet-permeable sterile cell culture plate according to claim 1, characterized in that, The ultraviolet lamp assembly (5) includes: The lamp carrier plate (51) is a flat plate structure; An ultraviolet bulb (52) is disposed on the lamp carrier plate (51), and the position of the ultraviolet bulb (52) corresponds to the position of the partition plate (2); A light shield (53) is disposed on the periphery of the lamp carrier plate (51) and is detachably connected to the lamp carrier plate (51).

8. The ultraviolet-permeable sterile cell culture plate according to claim 7, characterized in that: Each of the partitions (2) has 9 lamp mounting holes at its corresponding position.

9. The ultraviolet-permeable sterile cell culture plate according to claim 8, characterized in that: The nine lamp mounting holes are arranged such that three lamp mounting holes are set in each row and three lamp mounting holes are set in each column, and the distance between two adjacent lamp mounting holes in each row or column is the same.

10. The ultraviolet-permeable sterile cell culture plate according to claim 7, characterized in that, The ultraviolet lamp assembly (5) includes: The battery (8) is located below the lamp carrier plate (51) and is electrically connected to the ultraviolet bulb (52).

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