Separated half in-vitro culture device for researching subterraneous stems of plants

Abstract

The utility model relates to the technical field of plant subterranean stem culture, in particular to a separated half in-vitro culture device for studying plant subterranean stems, which comprises a box body, a covering component is arranged at the top of the box body, a partition plate is fixed in the box body, and the partition plate divides the inner wall of the box body into a first culture chamber and a second culture chamber. The subterranean stem fixing device comprises a partition plate, the partition plate is provided with slotted holes distributed at equal intervals, rubber parts are connected in the slotted holes in a clamped mode, and the rubber parts are provided with storage holes used for fixing subterranean stems, the ends, close to roots, of the subterranean stems are inserted into the storage holes of the rubber parts in a sliding mode, and then the rubber parts used for fixing the subterranean stems are sequentially fixed into the slotted holes in the partition plate. Multiple subterranean stems are treated at a time, the culture efficiency is improved, meanwhile, it is guaranteed that the treatment environment is completely consistent, and all phenotype indexes of the subterranean stems can be directly, continuously and dynamically observed in the culture period.

Description

Technical Field

[0001] This utility model relates to the field of plant underground stem culture technology, specifically to a semi-in vitro culture device for studying plant underground stems. Background Technology

[0002] Besides roots, some plants possess another special underground structure—the rhizome. The rhizome is a variant of the stem, and many plants rely on it for asexual reproduction, population expansion, and perennial traits. It plays a crucial role in agricultural production, weed control, and basic plant physiological research, particularly in agriculture. Current agricultural production is primarily based on annual food crops, but with global population growth and climate change, agricultural patterns are shifting, and the drawbacks of annual food crop cultivation are becoming increasingly apparent. Ecologically, tilling severely disrupts soil balance, leading to nutrient loss, soil erosion, and soil structure imbalances. Economically, it requires significant investment, including seeds, tools, and labor. Therefore, the continuous cultivation model has a negative impact on the ecological environment and is no longer entirely suitable for the current socio-economic situation, hindering sustainable agricultural development. To address these issues, shifting from annual to perennial food crop production is one of the most effective solutions. Currently, cultivated rice varieties capable of continuous growth have been successfully bred using perennial wild rice varieties with rhizomes, effectively promoting sustainable agricultural development. However, the mechanisms by which plants achieve perenniality through rhizomes are still unclear. Therefore, research on plant rhizomes is essential, not only to promote agricultural development and assist in weed control, but also to fill gaps in research on underground parts of plants. Currently, research on rhizomes is relatively lacking compared to that on above-ground parts, and a systematic methodology has not yet been established. Rhizome cultivation methods are mainly soil-based and hydroponically-based. Research on the effects of nutrient elements on the growth and development of rhizomes faces the following challenges:

[0003] 1. It is impossible to conduct continuous dynamic observation of the growth and development of the underground stem;

[0004] 2. The influence of other factors cannot be ruled out, including the environment and the condition of the above-ground parts of the plant. Utility Model Content

[0005] The purpose of this invention is to provide a semi-in vitro culture device for studying plant underground stems, in order to solve the problems mentioned in the background art.

[0006] The technical solution of this utility model is: a semi-in vitro culture device for studying plant underground stems, including a box, a cover assembly on the top of the box, a partition fixed inside the box, the partition dividing the inner wall of the box into a first culture chamber and a second culture chamber, slots evenly distributed on the partition, rubber parts snapped into the slots, and placement holes for fixing underground stems on the rubber parts.

[0007] The effect achieved by the above-mentioned components is as follows: by sliding one end of the underground stem near the root into the placement hole of the rubber component, and then fixing the rubber component that fixes the underground stem into the slots on the partition in sequence, multiple underground stems can be treated at once, improving the cultivation efficiency and ensuring complete consistency of the treatment environment. If the number of underground stems to be cultivated is less than the number of slots on the partition, an unperforated rubber component can be used for sealing. The end of the underground stem near the base is placed in the first cultivation chamber. Corresponding nutrient solutions are introduced into the first and second cultivation chambers respectively to simulate the nutrient environment above and below ground. Then, the covering component is placed on the top of the box. Finally, the device is placed in a greenhouse for light-blocking treatment to simulate the underground dark environment, or placed in a suitable environment as needed. During the cultivation period, various phenotypic indicators of the underground stem can be continuously and dynamically observed directly. After the cultivation is completed, subsequent experimental treatments can also be carried out to achieve the purpose of dynamic observation of multiple indicators, and no damage is caused to the underground stems during the process.

[0008] Preferably, the rubber component includes a T-shaped rubber plug, the placement hole is located at the center of the T-shaped rubber plug, the outer side of the T-shaped rubber plug is provided with a groove that engages with the slot, and one end of the T-shaped rubber plug is provided with a conical inclined surface.

[0009] The above-mentioned components achieve the following effects: the conical inclined surface facilitates the squeezing and pushing of the T-shaped rubber plug into the slot of the partition; the slot can tightly engage the T-shaped rubber plug in the slot, preventing the nutrient solution from flowing between the two sides of the partition; in addition, the placement hole can be opened with a corresponding type of hole opener, so as to tightly fit and fix the underground stems of different thicknesses.

[0010] Preferably, the top of the housing has two symmetrical sliding grooves, and the covering assembly is slidably inserted between the two sliding grooves.

[0011] The effect achieved by the above-mentioned components is that the sliding groove facilitates the sliding and fixing of the covering component.

[0012] Preferably, the covering assembly includes a cover plate with two sets of ventilation holes that communicate with the first culture chamber and the second culture chamber respectively, and a handle strip is fixed to one side of the bottom of the cover plate.

[0013] The above components achieve the following effects: the cover plate can cover the top of the chamber, and the vents can facilitate air exchange inside the chamber, preventing excessive evaporation of the internal nutrient solution while also increasing oxygen exchange.

[0014] Preferably, a foot is fixed at the bottom corner of the box, and the bottom of the foot has an anti-slip texture.

[0015] The effect achieved by the above components is that the feet can support the chamber, avoiding excessive contact area with the support base, which would cause heat exchange imbalance and affect the internal culture temperature of the chamber.

[0016] Preferably, the bottom of the box has two drain outlets that are respectively connected to the first culture chamber and the second culture chamber, and plugs are inserted into the drain outlets.

[0017] The aforementioned components achieve the following effect: by opening the plug, the nutrient solution in the first and second culture chambers can be discharged from the box without tilting the box significantly, thus fully protecting the underground stem and preventing breakage caused by violent shaking.

[0018] This invention provides an improved, semi-in vitro culture device for studying plant rhizomes, which has the following improvements and advantages compared with the prior art:

[0019] Firstly, this invention allows for the simultaneous treatment of multiple underground stems by sliding one end of the underground stem near the root into the placement hole of a rubber component, and then fixing the rubber component that fixes the underground stem into the slots on the partition. This improves cultivation efficiency and ensures a completely consistent treatment environment. If the number of underground stems to be cultivated is less than the number of slots on the partition, an unperforated rubber component can be used for sealing. The end of the underground stem near the base is placed in the first cultivation chamber. Corresponding nutrient solutions are introduced into the first and second cultivation chambers to simulate the nutrient environment above and below ground, respectively. The covering component is then placed on top of the box, and the device is placed in a greenhouse for light-blocking treatment to simulate the underground dark environment, or placed in a suitable environment as needed. During cultivation, various phenotypic indicators of the underground stem can be continuously and dynamically observed. After cultivation, subsequent experimental treatments can also be carried out to achieve the purpose of dynamic observation of multiple indicators, without causing damage to the underground stem during the process.

[0020] Secondly, the conical inclined surface of this invention facilitates the pressing and pushing of the T-shaped rubber plug into the slot of the partition. The slot can tightly engage the T-shaped rubber plug in the slot, preventing the nutrient solution from flowing between the two sides of the partition. In addition, the placement hole can be opened with a corresponding type of hole opener, so that underground stems of different thicknesses can be tightly fitted and fixed.

[0021] Thirdly, by culturing the underground stems after removing the above-ground parts, we can avoid the above-ground parts from transporting substances or transmitting signals to the underground stems, thus minimizing the influence of the above-ground parts on the underground stems, ensuring the singleness of experimental variables and the accuracy of results. Attached Figure Description

[0022] The present invention will be further explained below with reference to the accompanying drawings and embodiments:

[0023] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0024] Figure 2 This is a cross-sectional three-dimensional structural schematic diagram of the present invention;

[0025] Figure 3 This is a cross-sectional structural diagram of the rubber part in this utility model.

[0026] Explanation of reference numerals in the attached figures:

[0027] 1. Box body; 2. Slide groove; 3. Covering assembly; 31. Cover plate; 32. Vent hole; 33. Handle strip; 4. Foot pad; 5. First culture chamber; 6. Second culture chamber; 7. Partition; 8. Rubber parts; 81. T-shaped rubber plug; 82. Storage hole; 83. Slot; 84. Conical inclined surface; 9. Drain outlet; 10. Plug. Detailed Implementation

[0028] The present invention will now be described in detail, and the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0029] This invention provides an improved, semi-in vitro culture device for studying plant rhizomes. The technical solution of this invention is as follows:

[0030] In embodiments of this utility model, such as Figures 1-3As shown, a semi-in vitro culture device for studying plant rhizomes includes a box body 1. A foot 4 is fixed at the bottom corner of the box body 1, and the bottom of the foot 4 has anti-slip texture. The foot 4 supports the box body 1, preventing excessive contact area with the supporting base, which could cause heat exchange imbalance and affect the internal culture temperature of the box body 1. A cover assembly 3 is installed on the top of the box body 1. Two symmetrical sliding grooves 2 are opened on the top of the box body 1, and the cover assembly 3 slides between the two sliding grooves 2. The sliding grooves 2 facilitate the sliding and fixing of the cover assembly 3. The cover assembly 3 includes a cover plate 31, which has two sets of ventilation holes 32 communicating with the first culture chamber 5 and the second culture chamber 6 respectively. A handle 33 is fixed to one side of the bottom of the cover plate 31. The top of the box 1 can be covered, and the vent 32 can facilitate air exchange inside the box 1, preventing excessive evaporation of the nutrient solution and increasing oxygen exchange. A partition 7 is fixed inside the box 1, which divides the inner wall of the box 1 into a first culture chamber 5 and a second culture chamber 6. The partition 7 has equally spaced slots, and rubber parts 8 are snapped into the slots. The rubber parts 8 have placement holes 82 for fixing the underground stems. The rubber parts 8 include a T-shaped rubber plug 81. The placement hole 82 is located at the center of the T-shaped rubber plug 81. The outer side of the T-shaped rubber plug 81 has a groove 83 that engages with the slot. One end of the T-shaped rubber plug 81 has a conical inclined surface 84, which facilitates the squeezing of the T-shaped rubber plug 81. The T-shaped rubber plug 81 is pressed into the slot of the partition 7. The slot 83 can be used to tightly lock the T-shaped rubber plug 81 into the slot, preventing the nutrient solution from flowing between the two sides of the partition 7. In addition, the placement hole 82 can be opened with a corresponding type of hole opener to tightly fit and fix the underground stems of different thicknesses. By sliding the end of the underground stem near the root into the placement hole 82 of the rubber part 8, and then fixing the rubber parts 8 for fixing the underground stem in sequence into the slots on the partition 7, multiple underground stems can be treated at one time, while ensuring that the treatment environment is completely consistent. If the number of underground stems to be cultivated is less than the number of slots on the partition 7, an unopened rubber part 8 can be used for sealing. The end of the underground stem near the base is placed in the first culture chamber 5, and the nutrient solution is fed into the first culture chamber 5. Nutrient solutions are introduced into the first culture chamber 5 and the second culture chamber 6 to simulate the nutrient environment above and below ground, respectively. Then, the covering component 3 is placed on top of the box 1. Finally, the device is placed in a greenhouse for shading to simulate the underground dark environment, or placed in a suitable environment as needed. During the cultivation period, various phenotypic indicators of the underground stem can be continuously and dynamically observed directly. There are two drainage outlets 9 at the bottom of the box 1, which are connected to the first culture chamber 5 and the second culture chamber 6, respectively. A plug 10 is inserted into the drainage outlet 9. By opening the plug 10, the nutrient solution in the first culture chamber 5 and the second culture chamber 6 can be discharged from the box 1. There is no need to tilt the box 1 significantly to tilt it, which fully protects the underground stem and avoids breakage caused by violent shaking.

[0031] The working principle of the semi-in vitro culture device for studying plant underground stems provided by this utility model is as follows: The end of the underground stem near the root is slidably inserted into the placement hole 82 of the rubber component 8. The conical inclined surface 84 facilitates the pressing and pushing of the T-shaped rubber plug 81 into the slot of the partition 7. The slot 83 tightly engages the T-shaped rubber plug 81 within the slot, preventing cross-flow of nutrient solution between the two sides of the partition 7. Furthermore, the placement hole 82 can be opened using a corresponding type of hole opener to allow for tight fitting and fixing of underground stems of different thicknesses, enabling the processing of multiple underground stems at once, improving culture efficiency, and ensuring a completely consistent treatment environment. If the number of underground stems to be cultured is less than the number of slots on the partition 7, an unopened rubber component 8 can be used for sealing, with the end of the underground stem near the base positioned... In the first culture chamber 5, corresponding nutrient solutions are introduced into the first culture chamber 5 and the second culture chamber 6 respectively to simulate the nutrient environment above and below ground. Then, the covering component 3 is placed on top of the box 1. Finally, the device is placed in a greenhouse for light blocking to simulate the underground dark environment, or placed in a suitable environment as needed. The nutrient solution is changed every other day. The nutrient solution in the first culture chamber 5 and the second culture chamber 6 can be discharged from the box 1 by opening the plug 10. There is no need to tilt the box 1 significantly to tilt it, which fully protects the underground stem and avoids breakage caused by violent shaking. During the culture period, various phenotypic indicators of the underground stem can be continuously and dynamically observed. After the culture is completed, subsequent experimental treatments can be carried out to achieve the purpose of dynamic observation of multiple indicators, and no damage to the underground stem is caused in the process.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A semi-in vitro culture device for studying plant rhizomes, comprising a box (1), wherein a covering assembly (3) is provided on the top of the box (1), characterized in that: A partition (7) is fixed inside the box (1). The partition (7) divides the inner wall of the box (1) into a first culture chamber (5) and a second culture chamber (6). The partition (7) has equally spaced slots. A rubber part (8) is snapped into the slot. The rubber part (8) has a placement hole (82) for fixing the underground stem.

2. The semi-in vitro culture device for studying plant rhizomes according to claim 1, characterized in that: The rubber component (8) includes a T-shaped rubber plug (81), the storage hole (82) is located at the center of the T-shaped rubber plug (81), the outer side of the T-shaped rubber plug (81) is provided with a groove (83) that engages with the slot, and one end of the T-shaped rubber plug (81) is provided with a conical inclined surface (84).

3. The semi-in vitro culture device for studying plant rhizomes according to claim 1, characterized in that: The top of the box (1) has two symmetrical sliding grooves (2), and the covering component (3) is slidably inserted between the two sliding grooves (2).

4. The semi-in vitro culture device for studying plant rhizomes according to claim 3, characterized in that: The covering assembly (3) includes a cover plate (31), on which two sets of ventilation holes (32) are provided, which are respectively connected to the first culture chamber (5) and the second culture chamber (6). A handle strip (33) is fixed on one side of the bottom of the cover plate (31).

5. The semi-in vitro culture device for studying plant rhizomes according to claim 1, characterized in that: The bottom corner of the box (1) is fixed with a foot (4), and the bottom of the foot (4) has an anti-slip texture.

6. The semi-in vitro culture device for studying plant rhizomes according to claim 1, characterized in that: The bottom of the box (1) has two drain outlets (9) that are respectively connected to the first culture chamber (5) and the second culture chamber (6), and a plug (10) is inserted into the drain outlet (9).

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