Chromatographic column packing device and column chromatography equipment

Abstract

The utility model relates to a chromatographic column packing device and column chromatography equipment. The chromatographic column packing device comprises a connecting part, the bottom of the connecting part is magnetically connected to a chromatographic column, and the diameter of the cross section of the connecting part is consistent with that of the cross section of the chromatographic column; the bottom of the liquid carrying module is connected to the top of the connecting part, the diameter of the cross section of the liquid carrying module is consistent with that of the cross section of the connecting part, and the liquid carrying module can adjust the volume of the carried liquid. According to the scheme provided by the invention, the liquid required to be subjected to chromatography can be loaded in advance, excessive filling or insufficient filling of the liquid caused in a manual column filling process is avoided, and forward-inclined peaks or trailing peaks appearing in a map are reduced.

Description

Technical Field

[0001] This application relates to the field of solid phase extraction technology, and in particular to a column packing apparatus and column chromatography equipment. Background Technology

[0002] A chromatography column (also known as a chromatographic column) is the main component of gel chromatography technology. It is generally used in glass or plexiglass tubes and is a widely used tool, primarily for the separation and purification of compounds.

[0003] However, in actual chromatography processes, "leading peaks" or "tailing peaks" often appear in the chromatograms. Leading peaks are usually caused by overpacking the chromatography column, while tailing peaks are usually caused by underpacking. These problems are mainly due to overpacking or underpacking caused by manual column packing, as well as differences between the inlet and the inner diameter of the chromatography column. Utility Model Content

[0004] To address or partially address the problems existing in the related technologies, this application provides a chromatography column packing device and column chromatography equipment, which can pre-load the liquid to be chromatographically analyzed, avoiding over- or under-filling of liquid during manual column packing, and reducing the appearance of "forward-sloping peaks" or "tailing peaks" in the chromatogram.

[0005] The first aspect of this application provides a chromatography column packing apparatus, including a connecting part, the bottom of which is magnetically connected to the chromatography column, and the cross-sectional diameter of the connecting part is the same as the cross-sectional diameter of the chromatography column; and a liquid-carrying module, the bottom of which is connected to the top of the connecting part, and the cross-sectional diameter of the liquid-carrying module is the same as the cross-sectional diameter of the connecting part, and the liquid-carrying module is capable of adjusting the liquid volume.

[0006] In conjunction with the first aspect, one possible implementation of the first aspect further includes a liquid inlet section, the bottom of which is connected to the upper part of the liquid-carrying column, and the cross-sectional diameter of the liquid inlet section gradually increases from the bottom to the top.

[0007] In conjunction with the first aspect, in one possible implementation of the first aspect, a top cover is provided on the top of the liquid inlet.

[0008] In conjunction with the first aspect, in one possible implementation of the first aspect, the bottom of the connecting part is provided with a plurality of magnetic connecting rings of different diameters.

[0009] In conjunction with the first aspect, in one possible implementation of the first aspect, the liquid-carrying module includes a plurality of liquid-carrying columns, the surface of the liquid-carrying columns is provided with graduations, and the bottom of the liquid-carrying columns is threaded to the top of the connecting part; when the liquid-carrying module includes at least two liquid-carrying columns, the bottom of the liquid-carrying column is threaded to the top of the other liquid-carrying column.

[0010] In conjunction with the first aspect, in one possible implementation of the first aspect, the surface roughness of the inner surfaces of the liquid-carrying column and the connecting portion is less than 0.1 micrometers.

[0011] In conjunction with the first aspect, in one possible implementation of the first aspect, the liquid-carrying volume of the liquid-carrying column is 30-60 ml.

[0012] In conjunction with the first aspect, in one possible implementation of the first aspect, the length of the liquid-carrying column is 5-20 cm.

[0013] In conjunction with the first aspect, in one possible implementation of the first aspect, both the connecting part and the liquid-carrying module are cylindrical.

[0014] A second aspect of this application provides a column chromatography apparatus, including the column packing device for chromatography as described above.

[0015] The technical solution provided in this application may include the following beneficial effects:

[0016] The column packing apparatus and column chromatography equipment of this application include a connecting part, the bottom of which is magnetically connected to the chromatography column, and the cross-sectional diameter of the connecting part is the same as that of the chromatography column; and a liquid-carrying module, the bottom of which is connected to the top of the connecting part, and the cross-sectional diameter of the liquid-carrying module is the same as that of the connecting part. The liquid-carrying module can adjust the liquid volume and can pre-load the required chromatography liquid, avoiding over- or under-filling of liquid during manual column packing and reducing the appearance of "forward-tilting peaks" or "tailing peaks" in the spectrum.

[0017] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Attached Figure Description

[0018] The above and other objects, features and advantages of this application will become more apparent from the more detailed description of exemplary embodiments thereof in conjunction with the accompanying drawings, wherein the same reference numerals generally represent the same components in the exemplary embodiments thereof.

[0019] Figure 1 This is a schematic diagram of the structure of the column packing device and the connection state of the column in the chromatography embodiment shown in this application;

[0020] Figure 2 This is a schematic diagram of the threaded connection of the liquid-carrying column shown in the embodiments of this application;

[0021] Figure 3 This is another structural schematic diagram of the column packing device and the connection state of the chromatography column shown in the embodiments of this application.

[0022] Reference numerals: 1. Connecting part; 2. Chromatography column; 3. Liquid carrier module; 4. Liquid inlet; 5. Top cover; 6. Liquid carrier column. Detailed Implementation

[0023] Embodiments of this application will now be described in more detail with reference to the accompanying drawings. While embodiments of this application are shown in the drawings, it should be understood that this application may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to make this application more thorough and complete, and to fully convey the scope of this application to those skilled in the art.

[0024] It should be understood that although the terms "first," "second," "third," etc., may be used in this application to describe various information, this information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another. For example, without departing from the scope of this application, first information may also be referred to as second information, and similarly, second information may also be referred to as first information. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0025] Furthermore, in the embodiments of this application, "multiple" refers to two or more. Therefore, in the embodiments of this application, "multiple" can also be understood as "at least two". "At least one" can be understood as one or more, such as one, two, or more. For example, including at least one means including one, two, or more, and is not limited to which ones are included. For example, including at least one of A, B, and C, then it could include A, B, C, A and B, A and C, B and C, or A and B and C.

[0026] In the description of this application, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0027] Unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0028] A chromatography column (also known as a chromatographic column) is the main component of gel chromatography technology. It is generally used in glass or plexiglass tubes and is a widely used tool, primarily for the separation and purification of compounds.

[0029] A well-packed chromatography column 2 should exhibit complete symmetrical peaks in the chromatogram. However, due to equipment limitations, "leading peaks" or "tailing peaks" often appear in practice. Leading peaks are usually caused by overpacking of the chromatography column 2, while tailing peaks are usually caused by underpacking.

[0030] To address the aforementioned issues, this application provides a chromatography column packing device and a column chromatography apparatus, which can pre-load the liquid to be chromatographically analyzed, avoiding over- or under-filling of the liquid during manual column packing and reducing the appearance of "forward-sloping peaks" or "tailing peaks" in the chromatogram.

[0031] The technical solutions of the embodiments of this application are described in detail below with reference to the accompanying drawings.

[0032] Figure 1 This is a schematic diagram of the structure of the chromatography column packing device shown in the embodiments of this application.

[0033] See Figures 1-3 A chromatography column packing device includes a connecting part 1, the bottom of which is magnetically connected to a chromatography column 2. The cross-sectional diameter of the connecting part 1 is the same as that of the chromatography column 2. The bottom of the connecting part 1 can be magnetically connected to the upper end of the chromatography column 2, which facilitates the connection between the chromatography column packing device and the chromatography column 2. The fact that the cross-sectional diameter of the connecting part 1 is the same as that of the chromatography column 2 can maintain the same quality of the medium at the front and rear ends of the chromatography column 2, and avoid affecting the overall uniformity of the chromatography column 2.

[0034] The liquid-carrying module 3 is connected at its bottom to the top of the connecting part 1. The cross-sectional diameter of the liquid-carrying module 3 is the same as that of the connecting part 1. The liquid-carrying module 3 can adjust its liquid volume and can hold the liquid to be chromatographically analyzed. The volume of the liquid to be chromatographically analyzed can be predetermined, and the liquid volume of the liquid-carrying module 3 can be adjusted accordingly. Therefore, when performing chromatography, there is no need to manually add liquid again. The liquid-carrying module 3 stores the liquid to be chromatographically analyzed. The cross-sectional diameter of the liquid-carrying module 3 is the same as that of the connecting part 1, which can better control the flow rate of the liquid to keep the medium quality at the front and rear ends of the chromatography column 2 the same.

[0035] In one possible implementation, a liquid inlet 4 is also included. The bottom of the liquid inlet 4 is connected to the upper part of the liquid-carrying column 6. The cross-sectional diameter of the liquid inlet 4 gradually increases from the bottom to the top. The liquid inlet 4 is funnel-shaped, which helps personnel to control the liquid addition speed and thus control the amount of liquid added.

[0036] In one possible implementation, a top cover 5 is provided on the top of the liquid inlet 4. The top cover 5 can be used to cover the liquid inlet 4 and prevent dust from entering during chromatography.

[0037] In one possible implementation, the bottom of the connecting part 1 is provided with several magnetic connecting rings of different diameters. The diameters of the multiple magnetic connecting rings are different and can be selected according to the different diameters of the upper end of the chromatography column 2 so that the chromatography column packing device matches the chromatography column 2.

[0038] In one possible implementation, the liquid-carrying module 3 includes a plurality of liquid-carrying columns 6, the surface of which is provided with a scale, and the bottom of the liquid-carrying column 6 is threadedly connected to the top of the connecting part 1; when the liquid-carrying module 3 includes at least two liquid-carrying columns 6, the bottom of the liquid-carrying column 6 is threadedly connected to the top of another liquid-carrying column 6.

[0039] Specifically, the liquid-carrying column 6 has a certain liquid-carrying capacity. The volume of the liquid to be chromatographically analyzed can be calculated in advance. Based on the volume of the liquid and the liquid-carrying capacity of the liquid-carrying column 6, the number of liquid-carrying columns 6 can be determined. For example, the compression ratio of the liquid to be chromatographically analyzed can be calculated. When the compression ratio is 1.1, the volume of the liquid to be chromatographically analyzed is 1.1 times the liquid-carrying volume of the chromatography column 2. If the liquid-carrying volume of the chromatography column 2 is 100 ml, then the volume of the liquid to be chromatographically analyzed is 110 ml. When the liquid-carrying capacity of the liquid-carrying column 6 is 40 ml, three liquid-carrying columns 6 can be connected by threaded connection to obtain a liquid-carrying module 3 with a liquid-carrying volume of 120 ml, which can fully carry the volume of the liquid to be chromatographically analyzed. By pre-loading the liquid to be chromatographically analyzed, the over-filling or under-filling of liquid caused by manual column packing can be avoided, which can reduce the "asymmetric peak shape" in the spectrum, such as "forward-tilting peak" or "tailed peak".

[0040] Specifically, the actual volume of the liquid to be chromatographically analyzed can be calculated based on the compressibility factor of the liquid. The compressibility factor is the ratio of the settled bed height to the column height. For example, if the compressibility factor is 1.1, the actual volume of the liquid to be chromatographically analyzed is 1.1 times the column volume. In practice, the volume of the suspension should be twice the volume of the liquid to be chromatographically analyzed. The actual volume of the liquid to be chromatographically analyzed is estimated based on the compressibility factor of the chromatographic medium. The definition of the compressibility factor is the ratio of the settled bed height to the column height. This ratio can be used to estimate the actual volume of the liquid to be chromatographically analyzed. After obtaining the volume of the liquid to be chromatographically analyzed, the total volume of the chromatographic column and the carrier column can be calculated, and purified water can be added to the liquid to be chromatographically analyzed so that the total volume of the liquid to be chromatographically analyzed is equal to the total volume of the chromatographic column and the carrier column.

[0041] Specifically, the inner diameter of the liquid carrier column 6 is the same as that of the chromatography column 2, which can avoid the problem of "forward peak" caused by excessive compression of the liquid to be chromatographically processed when manually packed into the column.

[0042] In one possible implementation, the surface roughness of the inner surfaces of the liquid carrier column 6 and the connecting part 1 is less than 0.1 micrometers. The inner surfaces of the liquid carrier column 6 and the connecting part 1 are smooth and there are no tiny protrusions or depressions that would cause packing residue. This ensures that the liquid enters the chromatography column 2 from the liquid carrier column 6, thus guaranteeing the accuracy of the packing of the chromatography column 2.

[0043] In one possible implementation, the liquid-carrying volume of the liquid-carrying column 6 is 30-60 ml.

[0044] In one possible implementation, the length of the liquid-carrying column 6 is 5-20 cm.

[0045] In one possible implementation, both the connecting part 1 and the liquid-carrying module 3 are cylindrical. The cylindrical shape can better fit the shape of the chromatography column 2. Both the connecting part 1 and the liquid-carrying module 3 can be made of transparent plastic, which has good chemical stability and will not react chemically with various reagents in the chromatography process. At the same time, the transparent material makes it easy for the experimenter to observe the packing material during the column packing process.

[0046] Working principle:

[0047] First, calculate the volume of the chromatography column 2, then calculate the volume of the liquid to be chromatographically analyzed. After determining the volume of the liquid to be chromatographically analyzed, determine the number of liquid-carrying columns 6 in the liquid-carrying module 3 according to the required volume. When the number of liquid-carrying columns 6 is greater than 1, connect multiple liquid-carrying columns 6 by means of threaded connection. Then connect the liquid-carrying module 3 to the chromatography column 2 through the connecting part 1, adjust the liquid-carrying columns 6 and the chromatography column 2 to be on the same vertical line, add purified water to the liquid-carrying columns 6 through the liquid inlet part 4, and remove the purified water from the liquid-carrying columns 6 through the chromatography column 2 until the column bed interface is stable, and remove the chromatography column packing device. Add the liquid to be chromatographically analyzed to the chromatography column packing device and store it in the liquid-carrying columns 6, then connect the chromatography column packing device to the chromatography column 2, and then use the chromatography column 2 for chromatography.

[0048] The column packing apparatus of this application includes a connecting part 1, the bottom of which is magnetically connected to the column 2, and the cross-sectional diameter of the connecting part 1 is the same as that of the column 2; and a liquid-carrying module 3, the bottom of which is connected to the top of the connecting part 1, and the cross-sectional diameter of the liquid-carrying module 3 is the same as that of the connecting part 1. The liquid-carrying module 3 can adjust the liquid volume and can pre-load the required liquid for chromatography, avoiding over- or under-filling of liquid during manual column packing and reducing the appearance of "forward-tilting peaks" or "tailing peaks" in the spectrum.

[0049] This application also provides a column chromatography apparatus, including the column packing device for chromatography as described above.

[0050] The solution of this application has been described in detail above with reference to the accompanying drawings. In the above embodiments, the descriptions of each embodiment have different focuses; for parts not described in detail in a certain embodiment, please refer to the relevant descriptions of other embodiments. Those skilled in the art should also understand that the actions and modules involved in the specification are not necessarily essential to this application. Furthermore, it is understood that the steps in the method of this application embodiment can be adjusted, combined, and deleted according to actual needs, and the modules in the device of this application embodiment can be combined, divided, and deleted according to actual needs.

[0051] The various embodiments of this application have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A chromatography column packing apparatus, characterized in that, include: A connecting part, the bottom of which is magnetically connected to the chromatography column, and the cross-sectional diameter of the connecting part is the same as the cross-sectional diameter of the chromatography column; A liquid-carrying module is provided, with its bottom connected to the top of the connecting part. The cross-sectional diameter of the liquid-carrying module is the same as that of the connecting part, and the liquid-carrying module is capable of adjusting the liquid volume.

2. The chromatography column packing apparatus according to claim 1, characterized in that, It also includes a liquid inlet section, the bottom of which is connected to the upper part of the liquid-carrying module, and the cross-sectional diameter of the liquid inlet section gradually increases from the bottom to the top.

3. The chromatography column packing apparatus according to claim 2, characterized in that, The liquid inlet section is provided with a top cover.

4. The chromatography column packing apparatus according to claim 1, characterized in that, The bottom of the connecting part is provided with several magnetic connecting rings of different diameters.

5. The chromatography column packing apparatus according to claim 1, characterized in that, The liquid-carrying module includes several liquid-carrying columns, each with a graduation on its surface, and the bottom of each liquid-carrying column is threaded to the top of the connecting part. When the liquid-carrying module includes at least two liquid-carrying columns, the bottom of one liquid-carrying column is threadedly connected to the top of the other liquid-carrying column.

6. The chromatography column packing apparatus according to claim 5, characterized in that, The surface roughness of the inner surface of the liquid-carrying column and the connecting part is less than 0.1 micrometers.

7. The chromatography column packing apparatus according to claim 5, characterized in that, The liquid-carrying volume of the liquid-carrying column is 30-60 ml.

8. The chromatography column packing apparatus according to claim 5, characterized in that, The length of the liquid-carrying column is 5-20 cm.

9. The chromatography column packing apparatus according to claim 1, characterized in that, Both the connecting part and the liquid-carrying module are cylindrical.

10. A column chromatography apparatus, characterized in that, Includes the chromatography column packing apparatus as described in any one of claims 1-9.

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