Protein purification column convenient to clean

Abstract

The utility model relates to the technical field of protein purification, in particular to a protein purification column convenient to clean, which comprises a column body and a vibration component, the column body is divided into a mounting section and a purification section, a sieve plate is arranged in the purification section, and a filler column is arranged above the sieve plate; a driving cavity is formed in the mounting section, a collision convex block is arranged on the driving cavity, and a vibration assembly is mounted in the driving cavity; the vibration assembly generates vibration by colliding with the collision protruding block. Vibration can be stably generated through the vibration assembly, the vibration frequency is controlled according to the actual situation, the contact efficiency of NaCl and residual protein in the column body is improved, then the cleaning efficiency is improved, cleaning is more convenient, meanwhile, the sieve plate is cleaned through vibration, and the problem that the sieve plate is blocked is solved.

Description

Technical Field

[0001] This invention relates to the field of protein purification technology, specifically to a protein purification column that is easy to clean. Background Technology

[0002] Protein purification columns are based on the principle of separation. They utilize different separation media and adsorbents to selectively adsorb and elute target proteins, thereby achieving their separation and purification. Commonly used adsorbents include affinity agents, ion exchangers, gel filters, and dialysis membranes.

[0003] When using a protein purification column, a mixture sample is injected into the column. The various components in the mixture sample interact with the packing material inside the column. Depending on the interaction, the target protein can be selectively adsorbed onto the surface of the packing material, while other non-target components are eluted or flow out through the column, thereby achieving protein purification and separation.

[0004] After purification by a protein purification column, the target protein and other impurities may remain inside the column. These residues can accumulate and affect the efficiency and specificity of subsequent purification. Therefore, it is necessary to clean the protein purification column. However, the cleaning process for protein purification columns is cumbersome, time-consuming, and inconvenient.

[0005] In view of this, we propose a protein purification column that is easy to clean. Utility Model Content

[0006] The purpose of this invention is to provide a protein purification column that is easy to clean, so as to solve the problem of inconvenient cleaning of protein purification columns mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A protein purification column that is easy to clean includes a column body and a vibration assembly; the column body is divided into an installation section and a purification section. The installation section is used to fix the protein purification column on a clamp, and the purification section is used to purify the protein. The purification section has a sieve plate, and a packing column is provided above the sieve plate. The sieve plate serves as a support and filter, and the packing is used to separate specific proteins, thereby purifying the protein.

[0009] The installation section has a drive cavity, and the drive cavity has a collision protrusion. A vibration component is installed inside the drive cavity. The vibration component generates vibration by colliding with the collision protrusion. The vibration component and the collision protrusion generate stable vibration. The vibration force promotes the contact efficiency between NaCl and residual protein inside the column, thereby improving the cleaning efficiency of the column and ensuring the quality of cleaning.

[0010] Preferably, the installation section has symmetrical arc-shaped grooves that conform to the contour of the fingers, making it easy for staff to pick up and install the protein purification column and ensuring the stability of the protein purification column installation.

[0011] Preferably, the vibration assembly includes a drive column, a return spring, a drive wheel, and a vibration ring. The drive column is slidably installed in the drive cavity, with one end of the drive column outside the drive cavity and a drive protrusion on the end of the drive column inside the drive cavity. The drive column is connected to the inner wall of the drive cavity by a return spring, which is used to reset the drive column, thereby realizing the reciprocating motion of the drive column. The drive wheel is rotatably installed in the drive cavity and has a drive groove that cooperates with the drive column. When vibration is needed to speed up cleaning, the operator presses the drive column, and the drive column squeezes the drive groove on the drive wheel through the drive protrusion, thereby driving the drive wheel to rotate. A vibration ring is located below the drive wheel. The vibration ring has a vibration protrusion that cooperates with the collision protrusion. The drive wheel drives the vibration ring to rotate, and when the vibration ring rotates, the vibration protrusion on it contacts and collides with the collision protrusion, generating vibration to improve cleaning efficiency.

[0012] Preferably, the drive column has a pressing arc surface on one end outside the drive cavity. The pressing arc surface is used to improve the comfort of the operator and make it easier for the operator to press the drive column to drive the vibration ring to rotate and generate vibration.

[0013] Preferably, the vibrating ring includes a driving wheel, a driven wheel, a pawl, and a reciprocating spring; the driving wheel is rotatably mounted in the drive cavity and meshes with the drive wheel; a ratchet groove is formed on the inner circumference of the driving wheel; the driven wheel is located on the inner circumference of the driving wheel, and a slot is formed on the driven wheel for rotatably mounting the pawl; the pawl and the ratchet groove cooperate with each other, and the pawl and the slot are connected by a reciprocating spring, the return spring is used to realize the reciprocating motion of the pawl, a vibrating protrusion is provided on the inner circumference of the driven wheel, and the pawl of the driven wheel cooperates with the ratchet groove on the driving wheel to realize the unidirectional transmission of the driven wheel, and the inner circumference of the driven wheel is provided with a vibrating protrusion. The device is equipped with a vibration bump. When the drive wheel rotates forward under the action of the drive column, it drives the driving wheel to rotate synchronously. The ratchet groove on the driving wheel engages with the pawl. The driving wheel pushes the driven wheel to rotate through the pawl. The driven wheel drives the vibration bump to contact the collision bump, generating vibration. When the drive wheel reverses to reset, the driving wheel squeezes the pawl into the groove. When the ratchet groove on the driving wheel corresponds to the pawl, the reciprocating spring drives the pawl to reset. The unidirectional transmission between the driving wheel and the driven wheel ensures the stability of the vibration and avoids the driven wheel from rotating in both directions, which would lead to vibration superposition, resulting in unstable vibration and causing the packing to collapse.

[0014] Preferably, the vibrating protrusion has a hemispherical structure, and the collision protrusion has the same shape as the vibrating protrusion. The hemispherical structure reduces the friction when the vibrating protrusion and the collision protrusion come into contact with each other, thereby improving the rotation efficiency of the driven wheel, thus reducing the pressing force when pressing the drive column, and making it easier to drive the vibration assembly.

[0015] Compared with the prior art, the beneficial effects of this utility model are: this utility model can stably generate vibration through the vibration component and control the vibration frequency according to the actual situation, which improves the contact efficiency between NaCl and residual protein inside the column, thereby improving the cleaning efficiency and making it easier to clean. At the same time, the vibration cleans the sieve plate, avoiding the problem of the sieve plate being blocked. Attached Figure Description

[0016] Figure 1 This is a half-sectional schematic diagram of the protein purification column of this utility model;

[0017] Figure 2 This utility model Figure 1 Enlarged view of point A;

[0018] Figure 3 This is a top cross-sectional view of the protein purification column of this invention;

[0019] Figure 4 This utility model Figure 3 Enlarged view of point B;

[0020] Figure 5 This is a schematic diagram of the vibration component of this utility model;

[0021] Figure 6 This utility model Figure 5 Enlarged view of point C;

[0022] Figure 7 This is a schematic diagram of the vibration ring of this utility model.

[0023] In the picture:

[0024] 1. Column body; 11. Mounting section; 111. Drive chamber; 1111. Collision protrusion; 112. Arc groove; 12. Purification section; 121. Sieve plate; 122. Packing column;

[0025] 2. Vibration assembly; 21. Drive column; 211. Drive protrusion; 212. Pressing arc surface; 22. Return spring; 23. Drive wheel; 231. Drive groove; 24. Vibration ring; 241. Drive wheel; 2411. Ratchet; 242. Driven wheel; 2421. Vibration protrusion; 243. Pawl; 244. Reciprocating spring. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] When using a protein purification column, a mixture sample is injected into the column. The various components in the mixture sample interact with the packing material inside the column. Depending on the interaction, the target protein can be selectively adsorbed onto the surface of the packing material, while other non-target components are eluted or flow out through the column, thereby achieving protein purification and separation.

[0028] After purification by a protein purification column, the target protein and other impurities may remain inside the column. These residues can accumulate and affect the efficiency and specificity of subsequent purification. Therefore, it is necessary to clean the protein purification column. When cleaning the protein purification column, NaCl needs to be added to the column and allowed to stand for 10-15 minutes to remove proteins with strong ion adsorption. This makes the protein purification column cleaning process cumbersome, time-consuming, and inconvenient.

[0029] like Figures 1 to 7 As shown, a protein purification column that is easy to clean includes a column body 1 and a vibration assembly 2;

[0030] The column 1 is divided into an installation section 11 and a purification section 12. The purification section 12 contains a sieve plate 121, and a packing column 122 is provided above the sieve plate 121. The installation section 11 has a driving cavity 111, and the driving cavity 111 has a collision protrusion 1111. A vibration component 2 is installed in the driving cavity 111. The vibration component 2 generates vibration by colliding with the collision protrusion 1111.

[0031] Specifically, the mounting section 11 of the column 1 is used to fix the protein purification column on the fixture, and the purification section 12 is used for protein purification; the sieve plate 121 serves as a support and filter, supporting the packing material to prevent it from collapsing, and filtering out large particles of impurities. The packing material is used to separate specific proteins, thereby purifying the protein; the vibration component 2 collides with the collision protrusion 1111 to generate stable vibration, and the vibration force promotes the contact efficiency between NaCl and residual proteins inside the column 1, thereby improving the cleaning efficiency of the column 1 and ensuring the quality of cleaning. At the same time, the vibration is transmitted to the sieve plate 121 to clean the sieve plate 121 and prevent large particles of impurities from clogging the sieve plate 121;

[0032] Preferably, the vibration component 2 generates a fixed vibration force, which ensures the stability of NaCl movement and thus improves the stability of the contact between NaCl and the residual protein inside the column 1. This avoids the phenomenon of packing collapse caused by excessive vibration force. Compared with tapping the column 1 by hand, this ensures the stability of the packing and avoids the situation where the contact effect between NaCl and the residual protein inside the column 1 is not good due to insufficient force when tapping, or the packing collapses due to excessive force when tapping.

[0033] In this embodiment, arc-shaped grooves 112 are symmetrically provided on the mounting section 11;

[0034] Specifically, the arc-shaped groove 112 conforms to the contour of the finger, making it easy for staff to pick up and install the protein purification column, and ensuring the stability of the protein purification column installation.

[0035] In this embodiment, the vibration assembly 2 includes a drive column 21, a return spring 22, a drive wheel 23, and a vibration ring 24. The drive column 21 is slidably installed in the drive cavity 111, with one end of the drive column 21 located outside the drive cavity 111 and the other end of the drive column 21 located inside the drive cavity 111 having a drive protrusion 211. The drive column 21 is connected to the inner wall of the drive cavity 111 by the return spring 22. The drive wheel 23 is rotatably installed in the drive cavity 111, and the drive wheel 23 has a drive groove 231 that cooperates with the drive column 21. The vibration ring 24 is located below the drive wheel 23. The vibration ring 24 has a vibration protrusion 2421 that cooperates with the collision protrusion 1111.

[0036] Specifically, a limiting device is provided between the drive column 21 and the drive cavity 111, such as a limiting block on the drive column 21 and a limiting groove on the drive cavity 111. The limiting device is used to restrict the movement trajectory of the drive column 21 and ensure the stable operation of the drive column 21. The return spring 22 is used to reset the drive column 21, thereby realizing the reciprocating motion of the drive column 21, which in turn drives the drive wheel 23 to rotate in both directions. The drive wheel 23 is provided with a limiting ring, and the inner wall of the drive cavity 111 is provided with a limiting ring groove. The drive wheel 23 is rotatably mounted on the drive cavity 111 through the limiting ring. Inside cavity 111, when vibration is needed to speed up cleaning, the operator presses the drive column 21. The drive column 21, through the drive protrusion 211, presses the drive groove 231 on the drive wheel 23, thereby driving the drive wheel 23 to rotate. A vibration ring 24 is provided below the drive wheel 23. The vibration ring 24 is provided with a vibration protrusion 2421 that cooperates with the collision protrusion 1111. The drive wheel 23 drives the vibration ring 24 to rotate. When the vibration ring 24 rotates, the vibration protrusion 2421 on it contacts and collides with the collision protrusion 1111, generating vibration to improve cleaning efficiency.

[0037] In this embodiment, the driving column 21 has a pressing arc surface 212 on one end outside the driving cavity 111;

[0038] Specifically, the pressing arc surface 212 is used to improve the comfort of the staff and make it easier for the staff to press the drive column 21 to drive the vibration ring 24 to rotate and generate vibration.

[0039] In this embodiment, the vibrating ring 24 includes a driving wheel 241, a driven wheel 242, a pawl 243, and a reciprocating spring 244. The driving wheel 241 is rotatably mounted in the drive cavity 111 and meshes with the drive wheel 23. A ratchet groove 2411 is formed on the inner circumference of the driving wheel 241. The driven wheel 242 is located on the inner circumference of the driving wheel 241, and a slot is formed on the driven wheel 242 for rotatably mounting the pawl 243. The pawl 243 and the ratchet groove 2411 cooperate with each other, and the pawl 243 and the slot are connected by the reciprocating spring 244. A vibration protrusion 2421 is formed on the inner circumference of the driven wheel 242.

[0040] Specifically, the driving wheel 241 is arranged perpendicularly to the drive wheel 23, thus changing the direction of rotation and transforming the vertical rotation of the drive wheel 23 into the horizontal rotation of the driving wheel 241. A limiting ring groove is provided at the bottom of the drive cavity 111. Limiting rings are provided at the bottom of the driving wheel 241 and the driven wheel 242. The driving wheel 241 and the driven wheel 242 are rotatably mounted with the drive wheel 23 through the cooperation of the limiting rings and the limiting ring groove. The pawl 243 is rotatably mounted with the driven wheel 242 through a slot. An installation hole is provided in the slot of the driven wheel 242. A pin is mounted on the pawl 243, and the pawl 243 is rotatably mounted with the driven wheel 242 through the pin and the installation hole. When the drive wheel 23 rotates forward under the action of the drive column 21, the drive wheel... Drive wheel 23 drives drive wheel 241 to rotate synchronously. The ratchet groove 2411 on drive wheel 241 engages with pawl 243. Drive wheel 241 pushes driven wheel 242 to rotate through pawl 243. Drive wheel 242 drives vibrating protrusion 2421 to contact collision protrusion 1111 to generate vibration. When drive wheel 23 reverses and resets, drive wheel 241 squeezes pawl 243 into the groove. When ratchet groove 2411 on drive wheel 241 corresponds to pawl 243, reciprocating spring 244 drives pawl 243 to reset. The unidirectional transmission between drive wheel 241 and driven wheel 242 ensures the stability of vibration and avoids the driven wheel 242 from reversing, which would lead to vibration superposition, resulting in unstable vibration and causing packing collapse.

[0041] In this embodiment, the vibration bump 2421 is a hemispherical structure, and the collision bump 1111 has the same shape as the vibration bump 2421;

[0042] Specifically, the hemispherical structure reduces the friction between the vibrating protrusion 2421 and the collision protrusion 1111, thereby improving the rotation efficiency of the driven wheel 242, thus reducing the pressing force when pressing the drive column 21, and making it easier to drive the vibration assembly 2.

[0043] Preferably, the vibration bump 2421 is made of rubber material. The high elasticity of the rubber material can effectively absorb and release vibration, thereby enhancing the vibration generated when the vibration bump 2421 comes into contact with the collision bump 1111 and deforms.

[0044] In the use of this easy-to-clean protein purification column, the operator adds NaCl into the protein purification column, then presses the drive column 21. The drive column 21 compresses the reset spring 22. At the same time, the drive column 21 compresses the drive groove 231 on the drive wheel 23 through the drive protrusion 211, thereby driving the drive wheel 23 to rotate forward. The forward rotation of the drive wheel 23 drives the drive wheel 241 to rotate synchronously. The ratchet groove 2411 of the drive wheel 241 engages with the pawl 243, thereby driving the driven wheel 242 to rotate synchronously through the pawl 243. The driven wheel 242 drives the vibrating protrusion 2421 to contact the collision protrusion 1111 to generate vibration.

[0045] After the operator presses the drive shaft into the drive chamber 111, they release their finger. The reset spring 22 pushes the drive column 21 to reset. The drive column 21 drives the drive wheel 23 to reverse, and the drive wheel 23 drives the drive wheel 241 to reverse. The drive wheel 241 squeezes the pawl 243 into the slot of the driven wheel 242. The driven wheel 242 does not rotate and does not vibrate. After the drive shaft has been reset, it is pressed again.

[0046] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A protein purification column that is easy to clean, characterized in that: Includes a column (1) and a vibration assembly (2); The column (1) is divided into an installation section (11) and a purification section (12). The purification section (12) contains a sieve plate (121), and a packing column (122) is provided above the sieve plate (121). The installation section (11) is provided with a drive cavity (111), the drive cavity (111) is provided with a collision protrusion (1111), and a vibration component (2) is installed in the drive cavity (111). The vibration component (2) generates vibration by colliding with the collision bump (1111).

2. The protein purification column according to claim 1, characterized in that: The installation section (11) is symmetrically provided with arc-shaped grooves (112).

3. The protein purification column according to claim 1, characterized in that: The vibration assembly (2) includes a drive column (21), a return spring (22), a drive wheel (23), and a vibration ring (24). The drive column (21) is slidably installed with the drive cavity (111). One end of the drive column (21) is located outside the drive cavity (111), and a drive protrusion (211) is provided on the end of the drive column (21) located inside the drive cavity (111). The drive column (21) and the inner wall of the drive cavity (111) are connected by a return spring (22). The drive wheel (23) is rotatably mounted in the drive cavity (111). The drive wheel (23) has a drive groove (231) that cooperates with the drive column (21). A vibration ring (24) is provided below the drive wheel (23). The vibration ring (24) is provided with a vibration bump (2421) that cooperates with the collision bump (1111).

4. The protein purification column according to claim 3, characterized in that: The drive column (21) has a pressing arc surface (212) on one end outside the drive cavity (111).

5. The protein purification column according to claim 3, characterized in that: The vibrating ring (24) includes a driving wheel (241), a driven wheel (242), a pawl (243), and a reciprocating spring (244). The drive wheel (241) is rotatably mounted in the drive cavity (111) and meshes with the drive wheel (23); a ratchet groove (2411) is provided on the inner side of the circumference of the drive wheel (241). The driven wheel (242) is located inside the circumference of the driving wheel (241), and a slot is provided on the driven wheel (242) for rotating and installing the pawl (243). The pawl (243) and the ratchet groove (2411) cooperate with each other. The pawl (243) and the groove are connected by a reciprocating spring (244). The driven wheel (242) has a vibrating protrusion (2421) on its inner circumference.

6. The protein purification column according to claim 5, characterized in that: The vibration bump (2421) has a hemispherical structure, and the collision bump (1111) has the same shape as the vibration bump (2421).

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