Nucleic acid purification column

Abstract

The utility model discloses a nucleic acid purification column which comprises an adsorption column, a waste liquid pipe and fixing devices, the adsorption column is arranged above the waste liquid pipe, and the fixing devices are respectively arranged above the adsorption column and the waste liquid pipe and below the waste liquid pipe; the fixing device further comprises a top fixing mechanism and a base, the top fixing mechanism is arranged above the waste liquid pipe, and the base is arranged below the waste liquid pipe. The function of separating the adsorption column from the waste liquid pipe is added, and the adsorption column and the waste liquid pipe can be separated only by pressing a corresponding mechanism, so that the working efficiency is improved; a fixing and limiting function between parts is added, liquid in the device is prevented from leaking and polluting due to deviation of the parts in the centrifugal process, meanwhile, a new adsorption column and a waste liquid pipe can be replaced by the fixing mechanism to be repeatedly used, the safety and reliability of the device are improved, and the production cost is saved; and the base matched with the device is arranged, so that the device can be more conveniently placed on an operation surface during operation, and the working efficiency is improved.

Description

Technical Field

[0001] This invention belongs to the technical field of nucleic acid extraction devices, and particularly relates to a nucleic acid purification column. Background Technology

[0002] During the RNA extraction and sampling process, the virus sample needs to be mixed with the extraction solution, and then the mixture is added to the purification column. The sample is then centrifuged and extracted using a centrifuge. Finally, the sample obtained from the adsorption column is collected into a collection tube to obtain the viral RNA extract. During this process, the operator needs to remove the purification column multiple times and take the adsorption column from the waste liquid tube and place it into the collection tube to extract the sample.

[0003] In the prior art, such as the nucleic acid purification column disclosed in Chinese Patent (CN213141987U), a nucleic acid purification column includes a nucleic acid purification column and a waste liquid tube. The upper end of the nucleic acid purification column tapers to form a sample dispensing port, and the bottom of the nucleic acid purification column extends downward in the direction of decreasing cross-sectional area to form a liquid outlet. The outer surface of the nucleic acid purification column and the inner surface of the waste liquid tube are detachably connected by a snap-fit ​​structure. The inner wall of the nucleic acid purification column and the inner wall of the liquid outlet are completely covered with a nucleic acid adsorption porous membrane. The side of the cap that contacts the nucleic acid purification column has a chamber, and the inner wall of the chamber is covered with rubber. The chamber can seal the sample dispensing port when closed.

[0004] This method has the following drawbacks: First, the adsorption column needs to be repeatedly removed from the waste liquid tube and placed into new sample liquid to extract the sample. The removable part of the adsorption column in the existing solution is small, making it inconvenient to remove the adsorption column from the waste liquid tube. Second, during centrifugation, the adsorption column and the waste liquid tube are prone to displacement and separation under centrifugation, causing the liquid sample in the tube to flow out and cause contamination. Third, the purification column requires an additional fixing device to place it stably on the table, which increases the operating space, complicates the workflow, introduces additional operating steps, increases operating time, and reduces work efficiency.

[0005] Therefore, this paper provides a nucleic acid purification column. Utility Model Content

[0006] To address the aforementioned technical problems, this utility model discloses a nucleic acid purification column. This device adds a separation and removal function between the adsorption column and the waste liquid tube; simply pressing the corresponding mechanism separates the adsorption column and the waste liquid tube, improving work efficiency. It also adds a fixing and limiting function between components to prevent leakage and contamination of the liquid inside the device during centrifugation due to component deviation. Simultaneously, the fixing mechanism allows for replacement of new adsorption columns and waste liquid tubes for reuse, improving the safety and reliability of the device and saving production costs. Furthermore, a base adapted to the device is provided, allowing for easier placement of the device on the operating surface during operation, further improving work efficiency.

[0007] To achieve the above-mentioned technical effects, this utility model provides a nucleic acid purification column, including an adsorption column, a waste liquid tube, and a fixing device. The adsorption column is disposed above the waste liquid tube, and the fixing device is disposed above the adsorption column and below the waste liquid tube, respectively. The fixing device also includes a top fixing mechanism and a base. The top fixing mechanism is disposed above the waste liquid tube, and the base is disposed below the waste liquid tube.

[0008] Preferably, the adsorption column and the upper outer side of the waste liquid pipe are respectively provided with limiting grooves that match the top fixing mechanism.

[0009] Preferably, the top fixing mechanism further includes a fixing cover plate, a tube fixing mechanism, and a connecting strap. The fixing cover plate is disposed above the adsorption column, the tube fixing mechanism is disposed on the top side of the adsorption column and the top side of the waste liquid pipe, and the connecting strap is disposed on the side of the fixing cover plate with its upper end disposed on the upper end of the fixing cover plate and its lower end disposed on the right side of the tube fixing mechanism.

[0010] Preferably, the fixed cover plate further includes a housing a, a sealing ring, a limiting rod, a lever plate, and a leaf spring. The housing a is positioned above the adsorption column, the sealing ring is positioned between the top opening of the adsorption column and the housing a, the limiting rod is positioned inside the left side of the housing a, and the middle part of the limiting rod is rotatably connected to the housing a. The right end of the limiting rod is engaged with the limiting groove on the outside of the adsorption column. The lever plate is positioned at the left end of the limiting rod and is rotatably connected to the limiting rod at its lower end. The lever plate and the housing a are slidably connected through a groove on the housing a, and the leaf spring is positioned below the limiting rod.

[0011] Preferably, the tube fixing mechanism further includes a shell b, a limiting mechanism a, and a limiting mechanism b. The shell b is located below the shell a. The limiting mechanisms a are respectively located on the left and right sides of the upper part of the shell b. The limiting mechanisms b are respectively located on the front and rear sides of the upper part of the shell b.

[0012] Preferably, the limiting mechanism a further includes a limiting card a, a connecting rod a, a button a, a spring a, and a limiting protrusion a. The limiting card a is located inside the lower part of the outer shell b. The connecting rod a is located on the left side of the limiting card a and is hinged to the bottom end of the connecting rod a and the left end of the limiting card a. The middle part of the connecting rod a is rotatably connected to the outer shell b. The button a is located on the upper end of the connecting rod a and is hinged to the lower end of the button a and the upper end of the connecting rod a. The spring a is located on the right side of the button a. The limiting protrusion a is located on the right side of the limiting card a and matches the limiting groove on the adsorption column.

[0013] Preferably, the limiting mechanism b further includes a limiting card b, a connecting rod b, a button b, a spring b, and a limiting protrusion b. The limiting card b is located below the outer edge of the top of the waste liquid pipe. The connecting rod b is located in front of the limiting card b and is hinged to the bottom end of the connecting rod b and the left end of the limiting card b. The middle part of the connecting rod b is rotatably connected to the outer shell b. The button b is located at the upper end of the connecting rod b and is hinged to the lower end of the button b and the upper end of the connecting rod b. The spring a is located in front of the button a. The limiting protrusion b is located on the right side of the limiting card b and matches the limiting groove at the lower end of the outer edge of the top of the waste liquid pipe.

[0014] Preferably, a support platform is also provided on the left and right sides above the base.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] The device employs a fixing mechanism that separates and secures the adsorption column and waste liquid pipe. Simply pressing the corresponding mechanism allows for separation, improving work efficiency. The top fixing mechanism within the fixing device adds a limiting function between components, preventing leakage and contamination of the liquid inside the device during centrifugation due to component misalignment. Simultaneously, the fixing mechanism allows for the replacement of new adsorption columns and waste liquid pipes for reuse, improving the device's safety and reliability and saving production costs. The base within the fixing device allows for easier placement of the device on the operating surface during operation, further enhancing work efficiency. Attached Figure Description

[0017] Figure 1 This is a front view of the present invention;

[0018] Figure 2 yes Figure 1 A sectional view of section a.

[0019] Figure 3 yes Figure 2 A partial schematic diagram of b in the middle;

[0020] Figure 4 This is the left view of this utility model;

[0021] Figure 5 yes Figure 4 A sectional view of section c in the middle;

[0022] Figure 6 yes Figure 5 A partial schematic diagram of d in the middle;

[0023] Figure 7 This is the isometric drawing of this utility model.

[0024] The attached diagram lists the components represented by each number as follows:

[0025] 1. Adsorption column; 2. Waste liquid pipe; 3. Base; 4. Limiting groove; 5. Fixing cover plate; 6. Pipe body fixing mechanism; 7. Connecting belt; 8. Outer shell a; 9. Sealing ring; 10. Limiting rod; 11. Paddle plate; 12. Leaf spring; 13. Outer shell b; 14. Limiting clip a; 15. Connecting rod a; 16. Button a; 17. Spring a; 18. Limiting protrusion a; 19. Limiting clip b; 20. Connecting rod b; 21. Button b; 22. Spring b; 23. Limiting protrusion b; 24. Lifting platform. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0027] like Figures 1 to 7 As shown, the prior art in this embodiment has the following problems: The inventors have found the following defects in the prior art: 1. The adsorption column needs to be repeatedly taken out of the waste liquid tube and placed into new sample liquid to extract the sample. The removable part of the adsorption column in the existing solution is small, making it inconvenient to take the adsorption column out of the waste liquid tube; 2. During the centrifugation process of the purification column in the centrifuge, the adsorption column and the waste liquid tube are prone to displacement and separation under the centrifugation action, causing the liquid sample in the tube to flow out and cause contamination; 3. The purification column requires an additional fixing device to be placed stably on the table, which increases the operating space, complicates the workflow, generates additional operating steps, increases the operating time, and reduces work efficiency.

[0028] Therefore, the inventor provides a nucleic acid purification column, including an adsorption column 1, a waste liquid tube 2, and a fixing device. The adsorption column 1 is positioned above the waste liquid tube 2, and the fixing device is positioned above the adsorption column 1 and below the waste liquid tube 2, respectively. The fixing device also includes a top fixing mechanism and a base 3. The top fixing mechanism is positioned above the waste liquid tube 2, and the base 3 is positioned below the waste liquid tube 2.

[0029] Using the above method, during the preparation stage, the operator can first fix the adsorption column 1 and the waste liquid tube 2 (for adding reagents) in the top fixing mechanism, then place the device on the base 3, open the fixing cover 5, add the mixed solution for extracting RNA samples to the adsorption column 1, then close the fixing cover 5, and remove the fixing device along with the adsorption column 1 and waste liquid tube 2 into the centrifuge for centrifugation. During centrifugation, the fixing device provides partial fixation for the adsorption column 1 and waste liquid tube 2, preventing leakage and contamination of the liquid inside the device due to component displacement, thus improving the safety and reliability of the device. After centrifugation, remove the device from the centrifuge and place it back on the base 3, press the limiting mechanism b to release the adsorption column 1 and remove it from the waste liquid tube 2. The unsecured waste liquid tube 2 automatically falls onto the base 3 and can be removed from the side of the base 3. Then, the adsorption column 1 is transferred to the collection tube. Pressing the limiting mechanism a lowers the adsorption column 1, completing a single sample extraction. During the process, the operator holds the fixing device and can separate and replace the adsorption column 1 and waste liquid tube 2. Simply pressing the corresponding mechanism separates the adsorption column 1 and waste liquid tube 2, improving work efficiency and enabling the fixing device to be reused, thus saving production costs. After cleaning the fixing device, the remaining mixture is extracted using the same steps to complete the extraction of RNA samples from the mixed solution. Throughout the process, the base 3 allows for more convenient placement of the device on the operating surface, improving work efficiency.

[0030] Furthermore, the upper outer sides of the adsorption column 1 and the waste liquid pipe 2 are respectively provided with limiting grooves 4 that match the top fixing mechanism;

[0031] The limiting groove 4 on the adsorption column 1 can prevent the adsorption column 1 from shifting during centrifugation while fixing the adsorption column 1 to the top fixing mechanism. The limiting groove 4 on the waste liquid pipe 2 can prevent the waste liquid pipe 2 from shifting during centrifugation while fixing the waste liquid pipe 2 to the top fixing mechanism.

[0032] Furthermore, the top fixing mechanism also includes a fixing cover plate 5, a tube fixing mechanism 6, and a connecting strap 7. The fixing cover plate 5 is located above the adsorption column 1. The tube fixing mechanism 6 is located on the top side of the adsorption column 1 and the top side of the waste liquid pipe 2, respectively. The connecting strap 7 is located on the side of the fixing cover plate 5, with the upper end of the connecting strap 7 located on the upper end of the fixing cover plate and the lower end of the connecting strap 7 located on the right side of the tube fixing mechanism 6.

[0033] The fixed cover plate 5 can fix the top of the adsorption column 1, and can be opened when adding solution. It can ensure that the internal solution will not flow out and cause contamination during centrifugation. The tube fixing mechanism 6 can fix the inserted adsorption column 1 and waste liquid tube 2. The connecting strap 7 is used to connect the fixed cover plate 5 and the tube fixing mechanism 6. The fixed cover plate 5 can provide a fulcrum for gripping when moving the purification column.

[0034] Furthermore, the fixed cover plate 5 also includes a housing a8, a sealing ring 9, a limiting rod 10, a lever 11, and a leaf spring 12. The housing a8 is located above the adsorption column 1. The sealing ring 9 is located between the top opening of the adsorption column 1 and the housing a8. The limiting rod 10 is located inside the left side of the housing a8, and the middle part of the limiting rod 10 is rotatably connected to the housing a8. The right end of the limiting rod 10 is engaged with the limiting groove 4 on the outside of the adsorption column 1. The lever 11 is located at the left end of the limiting rod 10 and is rotatably connected to the limiting rod 10 at its lower end. The lever 11 is slidably connected to the housing a8 through a sliding groove on the housing a8. The leaf spring 12 is located below the limiting rod 10.

[0035] The limiting rod 10 achieves its limiting function by engaging its right end into the limiting groove 4 on the adsorption column 1. The leaf spring 12 provides elastic support for the limiting rod 10 from below. When the cover needs to be opened, the operator moves the lever 11 upwards. The lever 11 drives the limiting rod 10 to rotate downwards about the connection point between the limiting rod 10 and the middle of the outer shell a8, causing the right end of the limiting rod 10 to disengage from the limiting groove 4. Then, the fixed cover 5 can be opened by lifting it upwards. To close the fixed cover 5, simply install the fixed cover 5 downwards, and the adsorption column 1 presses against the limiting card, causing the limiting card to rise upwards until it engages in the limiting groove 4. The leaf spring 12 then causes the limiting card to spring back, thus completing the sealing and limiting of the adsorption column 1.

[0036] Furthermore, the pipe fixing mechanism 6 also includes a housing b13, a limiting mechanism a, and a limiting mechanism b. The housing b13 is located below the housing a8. The limiting mechanisms a are respectively located on the left and right sides of the upper part inside the housing b13, and the limiting mechanisms b are respectively located on the front and rear sides of the upper part inside the housing b13.

[0037] Among them, limiting mechanism a can fix and limit the adsorption column 1 installed in the fixing device, and limiting mechanism b can fix and limit the waste liquid pipe 2 installed in the fixing device. The limiting mechanism a and limiting mechanism b are arranged separately in the left and right and front and back directions of the outer shell b13, which increases the space for operation and gripping on the fixing device. At the same time, the adsorption column 1 and waste liquid pipe 2 can be separated by simply pressing the corresponding mechanism, thus improving work efficiency.

[0038] Furthermore, the limiting mechanism a also includes a limiting card a14, a connecting rod a15, a button a16, a spring a17, and a limiting protrusion a18. The limiting card a14 is located inside the lower part of the outer shell b13. The connecting rod a15 is located on the left side of the limiting card a14 and is hinged to the bottom end of the connecting rod a15 and the left end of the limiting card a14. The middle part of the connecting rod a15 is rotatably connected to the outer shell b13. The button a16 is located on the upper end of the connecting rod a15 and is hinged to the lower end of the button a16 and the upper end of the connecting rod a15. The spring a17 is located on the right side of the button a16. The limiting protrusion a18 is located on the right side of the limiting card a14 and matches the limiting groove 4 on the adsorption column 1.

[0039] In the fixed state, the limiting protrusion a18 on the right end of the limiting card a14 engages with the limiting groove 4 on the adsorption column 1. The spring a17 provides a leftward elastic force to the button a16. The spring a17 converts the elastic force into a rightward supporting force through the connecting rod a15. At this time, the limiting card a14 engages with the limiting groove 4 on the adsorption column 1 to prevent the adsorption column 1 from shifting during centrifugation. When it is necessary to release the fixation, the button a16 is pressed. The button a16 drives the connecting rod a15 to rotate around the connection between the connecting rod a15 and the outer shell b13, causing the limiting card a14 to lift to the left, releasing the restriction on the adsorption column 1 and removing the adsorption column 1 from the fixing device. When it is necessary to install the adsorption column 1, simply press the button a16 to align and install the adsorption column 1.

[0040] Furthermore, the limiting mechanism b also includes a limiting card b19, a connecting rod b20, a button b21, a spring b22, and a limiting protrusion b23. The limiting card b19 is located below the top outer edge of the waste liquid pipe 2. The connecting rod b20 is located in front of the limiting card b19 and is hinged to the bottom end of the connecting rod b20 and the left end of the limiting card b19. The middle part of the connecting rod b20 is rotatably connected to the outer shell b13. The button b21 is located at the upper end of the connecting rod b20 and is hinged to the lower end of the button b21 and the upper end of the connecting rod b20. The spring a17 is located in front of the button a16. The limiting protrusion b23 is located on the right side of the limiting card b19 and matches the limiting groove 4 at the lower end of the top outer edge of the waste liquid pipe 2.

[0041] In the fixed state, the limiting protrusion b23 on the right end of the limiting card b19 engages with the limiting groove 4 on the adsorption column 1. The spring b22 provides a forward elastic force to the button b21. The spring b22 converts the elastic force into a backward supporting force through the connecting rod b20. At the same time, the limiting card b19 engages below the top outer edge of the waste liquid tube 2, fixing the waste liquid tube 2. Simultaneously, the limiting protrusion b23 at the front end of the limiting card b19 engages with the limiting groove on the waste liquid tube 2, preventing the waste liquid tube 2 from shifting during centrifugation. When it is necessary to release the fixation, press the button b21. The button b21 drives the connecting rod b20 to rotate around the connection between the connecting rod b20 and the outer shell b13, causing the limiting card b19 to lift forward, releasing the restriction on the waste liquid tube 2 and removing the waste liquid tube 2 from the fixing device. When it is necessary to install the waste liquid tube 2, simply press the button b21 to align and install the waste liquid tube 2.

[0042] Furthermore, support platforms 24 are provided on the left and right sides above the base 3;

[0043] The waste liquid pipe 2 will fall onto the lifting platform 24 after it is released from its fixed position. The waste liquid pipe 2 can be temporarily placed on the lifting platform 24. When it is necessary to move the waste liquid pipe 2, it can be picked up from the space in the middle of the lifting platform 24.

[0044] In summary, this device employs a fixing mechanism that separately secures the adsorption column 1 and the waste liquid pipe 2. Simply pressing the corresponding mechanism allows for partial separation of the adsorption column 1 and waste liquid pipe 2, improving work efficiency. The top fixing mechanism in the fixing device adds a fixing and limiting function between components, preventing leakage and contamination of the liquid inside the device during centrifugation due to component deviation. Simultaneously, the fixing mechanism allows for the replacement of new adsorption columns 1 and waste liquid pipe 2 for reuse, improving the safety and reliability of the device and saving production costs. The base 3 in the fixing device allows for more convenient placement of the device on the operating surface during operation, further improving work efficiency.

[0045] The working principle of this utility model:

[0046] During the preparation phase, the operator can first fix the adsorption column 1 and the waste liquid tube 2 containing the reagent into the top fixing mechanism. The limiting protrusion a18 on the right end of the limiting clip a14 engages with the limiting groove 4 on the adsorption column 1. The spring a17 provides a leftward elastic force to the button a16. The spring a17 converts the elastic force into a rightward supporting force through the connecting rod a15. At the same time, the limiting clip a14 engages with the limiting groove 4 on the adsorption column 1 to prevent the adsorption column 1 from shifting during centrifugation. The limiting protrusion b23 on the right end of the time limit card b19 is engaged in the limiting groove 4 on the adsorption column 1. The spring b22 provides a forward elastic force to the button b21. The spring b22 converts the elastic force into a backward supporting force through the connecting rod b20. The time limit card b19 is engaged below the top outer edge of the waste liquid tube 2 to fix the waste liquid tube 2. At the same time, the limiting protrusion b23 at the front end of the time limit card b19 is engaged in the limiting groove on the waste liquid tube 2 to prevent the waste liquid tube 2 from shifting during centrifugation.

[0047] The device is then placed on the base 3. The operator moves the lever 11 upwards, causing the limiting rod 10 to rotate downwards around the connection point between the limiting rod 10 and the middle of the outer shell a8, so that the right end of the limiting rod 10 disengages from the limiting groove 4. Then, the fixing cover 5 is lifted upwards to open the fixing cover 5. The mixed solution for extracting RNA samples is added to the adsorption column 1. The fixing cover 5 is then closed, and the fixing device, along with the adsorption column 1 and the waste liquid tube 2, is taken out and placed in a centrifuge for centrifugation. During centrifugation, the fixing device provides fixation for the adsorption column 1 and the waste liquid tube 2, preventing leakage and contamination of the liquid inside the device due to the deviation of the components during centrifugation, thus improving the safety and reliability of the device.

[0048] After centrifugation, remove the device from the centrifuge and place it back on the base 3. Press button b21. Button b21 drives the connecting rod b20 to rotate around the connection between the connecting rod b20 and the outer shell b13, causing the limit card b19 to lift forward, releasing the restriction on the waste liquid tube 2. After being released, the waste liquid tube 2 will fall onto the lifting platform 24. The waste liquid tube 2 can be temporarily placed on the lifting platform 24. When it is necessary to transfer the waste liquid tube 2, it can be picked up from the space in the middle of the lifting platform 24. Then, transfer the adsorption column 1 into the collection tube and press... Button A16 drives connecting rod A15 to rotate around the connection point between connecting rod A15 and outer casing B13, causing limit card A14 to lift to the left, releasing the restriction on adsorption column 1 and removing adsorption column 1 from the fixing device, completing a single sample extraction. This adds the function of separating and retrieving adsorption column 1 and waste liquid tube 2. Only the corresponding mechanism needs to be pressed to partially separate adsorption column 1 and waste liquid tube 2, improving work efficiency. At the same time, the fixing mechanism can be replaced with new adsorption column 1 and waste liquid tube 2 for reuse, saving production costs.

[0049] After cleaning the fixation device, the remaining mixture was extracted using the same steps to complete the extraction of RNA samples from the mixture. Throughout the process, the base 3 allows for easier placement of the device on the operating surface, improving work efficiency.

[0050] This concludes the description of the operating principle of the device.

[0051] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0052] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid purification column, comprising an adsorption column (1), a waste liquid tube (2), and a fixing device, wherein the adsorption column (1) is disposed above the waste liquid tube (2), and the fixing device is disposed above the adsorption column (1) and below the waste liquid tube (2), respectively, characterized in that: The fixing device also includes a top fixing mechanism and a base (3). The top fixing mechanism is located above the waste liquid pipe (2), and the base (3) is located below the waste liquid pipe (2).

2. The nucleic acid purification column according to claim 1, characterized in that: The upper outer sides of the adsorption column (1) and the waste liquid pipe (2) are respectively provided with limiting grooves (4) that match the top fixing mechanism.

3. The nucleic acid purification column according to claim 1, characterized in that: The top fixing mechanism also includes a fixing cover plate (5), a tube fixing mechanism (6), and a connecting strap (7). The fixing cover plate (5) is set above the adsorption column (1). The tube fixing mechanism (6) is set on the top side of the adsorption column (1) and the top side of the waste liquid pipe (2). The connecting strap (7) is set on the side of the fixing cover plate (5), with the upper end of the connecting strap (7) set on the upper end of the fixing cover plate and the lower end of the connecting strap (7) set on the right side of the tube fixing mechanism (6).

4. A nucleic acid purification column according to claim 3, characterized in that: The fixed cover plate (5) also includes a shell a (8), a sealing ring (9), a limiting rod (10), a lever (11), and a leaf spring (12). The shell a (8) is located above the adsorption column (1). The sealing ring (9) is located between the top opening of the adsorption column (1) and the shell a (8). The limiting rod (10) is located inside the left side of the shell a (8), and the middle part of the limiting rod (10) is rotatably connected to the shell a (8). The right end of the limiting rod (10) is engaged with the limiting groove (4) on the outside of the adsorption column (1). The lever (11) is located at the left end of the limiting rod (10) and is rotatably connected to the limiting rod (10) at the lower end of the lever (11). The lever (11) is slidably connected to the shell a (8) through the sliding groove on the shell a (8). The leaf spring (12) is located below the limiting rod (10).

5. A nucleic acid purification column according to claim 3, characterized in that: The tube fixing mechanism (6) further includes a shell b (13), a limiting mechanism a, and a limiting mechanism b. The shell b (13) is located below the shell a (8). The limiting mechanism a is located on the left and right sides of the upper part of the shell b (13), and the limiting mechanism b is located on the front and rear sides of the upper part of the shell b (13).

6. A nucleic acid purification column according to claim 5, characterized in that: The limiting mechanism a further includes a limiting card a (14), a connecting rod a (15), a button a (16), a spring a (17), and a limiting protrusion a (18). The limiting card a (14) is located inside the lower part of the outer shell b (13). The connecting rod a (15) is located on the left side of the limiting card a (14) and is hinged to the bottom end of the connecting rod a (15) and the left end of the limiting card a (14). The middle part of the connecting rod a (15) is rotatably connected to the outer shell b (13). The button a (16) is located on the upper end of the connecting rod a (15) and is hinged to the lower end of the button a (16) and the upper end of the connecting rod a (15). The spring a (17) is located on the right side of the button a (16). The limiting protrusion a (18) is located on the right side of the limiting card a (14) and matches the limiting groove (4) on the adsorption column (1).

7. A nucleic acid purification column according to claim 5, characterized in that: The limiting mechanism b also includes a limiting card b (19), a connecting rod b (20), a button b (21), a spring b (22), and a limiting protrusion b (23). The limiting card b (19) is located below the top outer edge of the waste liquid pipe (2). The connecting rod b (20) is located in front of the limiting card b (19) and is hinged between the bottom end of the connecting rod b (20) and the left end of the limiting card b (19). The middle part of the connecting rod b (20) is rotatably connected to the outer shell b (13). The button b (21) is located at the upper end of the connecting rod b (20) and is hinged between the lower end of the button b (21) and the upper end of the connecting rod b (20). The spring a (17) is located in front of the button a (16). The limiting protrusion b (23) is located on the right side of the limiting card b (19) and matches the limiting groove (4) at the lower end of the top outer edge of the waste liquid pipe (2).

8. A nucleic acid purification column according to claim 1, characterized in that: The base (3) is also provided with support platforms (24) on the left and right sides above it.

Images