Sampling device used for bioreactor and capable of being repeatedly sterilized

Abstract

The utility model discloses a sampling device used for a bioreactor and capable of being repeatedly sterilized, and particularly relates to the technical field of bioreactor sampling, the sampling device comprises a sampling barrel, a sampling assembly is arranged on the sampling barrel, the sampling assembly comprises a discharge pipe orifice arranged on the outer side of the sampling barrel, a piston column is arranged in the sampling barrel, and the piston column is connected with the discharge pipe orifice. And a positioning block is arranged at one end of the piston column. According to the quantitative sampling device, the sampling assembly is arranged, the push rod is rotated to drive the positioning block, the material taking pipe fitting and the material taking cavities to rotate, then samples flow into the material taking cavities to be stored, the function of quantitative sampling of the samples is achieved, and the samples in the material taking cavities can be discharged through the discharging pipe opening by rotating the push rod; the sampling precision is ensured.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor sampling technology, and more specifically, to a sampling device for reusable sterilization in bioreactors. Background Technology

[0002] During aseptic culture, it is often necessary to sample the culture medium within the bioreactor. This typically includes aseptic sampling for aseptic monitoring and routine sampling to perform physicochemical analysis on indicators such as microbial or cell morphology, concentration, and product content. During the culture process, sampling is generally performed at least once a day or multiple times a day. The aseptic testing and physicochemical parameter analysis of the samples help determine the growth status of microorganisms or cells within the bioreactor, providing a basis for subsequent adjustment of culture parameters. Strict aseptic control must be maintained during both aseptic and routine sampling; otherwise, product contamination within the bioreactor can occur, resulting in irreparable and substantial economic losses.

[0003] Among them, the patent with announcement number CN219489998U discloses a sampling device for reusable sterilization of bioreactors, including a sterilization component with an air inlet connected to an external steam pipe and a bioreactor connected to the sterilization component; a sampling component with an air inlet connected to the air outlet of the sterilization component and an air outlet section, the sampling component including a sampling bottle, a three-way valve and an exhaust pipe, a valve pipe two connected to the exhaust pipe on the side of the three-way valve, a valve first connected in series on the exhaust pipe; a valve pipe three connected to the bottom of the three-way valve, and a connector connected to the valve pipe three on the top of the sampling bottle;

[0004] When in use, this structure sterilizes the samples offline and connects to the sampling port of the bioreactor. This allows the bioreactor to be sterilized simultaneously with the sampling pipes of the aseptic sampling device, ensuring the sterility of the sampling pipes, effectively avoiding energy waste, and reducing the workload of the staff. However, when sampling, the sample is prone to backflow, resulting in poor sampling results. Utility Model Content

[0005] In order to overcome the above-mentioned defects of the prior art, the present invention provides a sampling device for reusable sterilization in bioreactors, which aims to solve the problems mentioned in the background art.

[0006] This utility model provides the following technical solution: a sampling device for reusable sterilization of bioreactors, comprising a sampling cylinder, wherein a sampling component is provided on the sampling cylinder;

[0007] The sampling assembly includes a discharge port located on the outside of the sampling cylinder. A piston column is located inside the sampling cylinder. A positioning block is located at one end of the piston column. A material receiving tube is fixedly located on the outside of the positioning block. Two material receiving cavities are opened on the material receiving tube. A piston disc is located at the end of the positioning block away from the piston column. A push rod is located in the middle of the piston column, the positioning block, and the piston disc. One end of the push rod extends to the outside of the sampling cylinder.

[0008] As can be seen, in the above technical solution, the sample flows between the piston cylinder and the piston disc through the guide cavity between the piston disc and the piston rod. Rotating the push rod causes the positioning block, the sampling tube, and the sampling chamber to rotate, allowing the sample to flow into each sampling chamber for storage, thus achieving quantitative sampling. Afterwards, pulling back the push rod allows the sampling chamber to be transported to the discharge port. Rotating the push rod allows the sample in each sampling chamber to be discharged through the discharge port, ensuring sampling accuracy. Furthermore, when the sampling chamber and sampling tube are transported to the discharge port, the piston disc is positioned inside the sampling cylinder to seal it, preventing sample spillage.

[0009] Optionally, in one possible implementation, the vertical cross-sectional shape of the discharge pipe is set to be conical, and the discharge pipe is connected to the sampling cylinder. A disinfection pipe is sleeved on the discharge pipe, and the disinfection pipe is connected to the discharge pipe by a clamp. A valve is provided on the disinfection pipe. A connecting sleeve is sleeved on the outside of the push rod. The connecting sleeve is located at one end of the sampling cylinder and is engaged with the sampling cylinder. An extension tube is fixedly provided at the end of the sampling cylinder away from the connecting sleeve. A guide cavity is formed between the extension tube and the piston disc.

[0010] As can be seen, in the above technical solution, an external water pipe is connected to the disinfection pipe opening, and then the valve is opened to allow water to flow into the sampling cylinder to rinse and disinfect the various structures inside the sampling cylinder, thus avoiding inaccurate sampling caused by repeated use.

[0011] The technical effects and advantages of this utility model are as follows:

[0012] By setting up a sampling component, the sample can flow between the piston column and the piston disc through the guide cavity between the piston disc and the extension tube. By rotating the push rod, the push rod drives the positioning block, the material picking tube and the material picking cavity to rotate, thereby allowing the sample to flow into each material picking cavity for storage, thus realizing the function of quantitative sampling of the sample.

[0013] By pulling back the push rod, the sampling chamber can be transported to the discharge port. By rotating the push rod, the samples in each sampling chamber can be discharged through the discharge port, ensuring sampling accuracy. When the sampling chamber and sampling tube are transported to the discharge port, the piston disc can be located inside the sampling cylinder to achieve the function of sealing the sampling cylinder and preventing the sample from spilling out. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in this disclosure, the accompanying drawings used in some embodiments will be briefly described below. Obviously, the drawings described below are only drawings of some embodiments of this disclosure, and those skilled in the art can obtain other drawings based on these drawings. In addition, the drawings described below can be regarded as schematic diagrams and are not intended to limit the actual size of the product, the actual flow of the method, the actual timing of the signals, etc. involved in the embodiments of this disclosure.

[0015] Figure 1 This is a front view of the overall structure of this utility model.

[0016] Figure 2 This is a cross-sectional view of the overall structure of this utility model.

[0017] Figure 3 This is a perspective view of the discharge pipe, sampling cylinder, extension pipe, disinfection pipe and valve of this utility model.

[0018] Figure 4 This is a perspective view of the push rod, piston column, positioning block, piston disc, and material handling tube of this utility model.

[0019] The attached diagram is labeled as follows: 1. Sampling cylinder; 2. Discharge port; 3. Piston column; 4. Positioning block; 5. Material taking fitting; 6. Material taking chamber; 7. Piston disc; 8. Push rod; 9. Disinfection port; 10. Valve; 11. Connecting sleeve; 12. Extension pipe. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] As attached Figure 1 - Figure 4The sampling device shown is for reusable sterilization in a bioreactor. Through the sampling components on the sampling cylinder 1, the sample flows between the piston column 3 and the piston disk 7 via the guide cavity between the piston disk 7 and the extension tube 12. Rotating the push rod 8 causes the positioning block 4, the material collection tube 5, and the material collection chamber 6 to rotate, allowing the sample to flow into each material collection chamber 6 for storage, thus achieving quantitative sampling. Afterwards, pulling back the push rod 8 allows the material collection chamber 6 to be transported to the discharge port 2. Rotating the push rod 8 allows the sample in each material collection chamber 6 to be discharged through the discharge port 2, ensuring sampling accuracy. When the material collection chamber 6 and the material collection tube 5 are transported to the discharge port 2, the piston disk 7 is positioned inside the sampling cylinder 1 to seal the sampling cylinder 1, preventing sample spillage. The specific structural configuration of the components is as follows.

[0022] The sampling assembly includes a discharge port 2 located on the outside of the sampling cylinder 1, a piston column 3 located inside the sampling cylinder 1, a positioning block 4 located at one end of the piston column 3, a material taking pipe 5 fixedly located on the outside of the positioning block 4, two material taking cavities 6 opened on the material taking pipe 5, a piston disc 7 located at the end of the positioning block 4 away from the piston column 3, and a push rod 8 located in the middle of the piston column 3, the positioning block 4 and the piston disc 7, with one end of the push rod 8 extending to the outside of the sampling cylinder 1.

[0023] The vertical cross-section of the discharge pipe 2 is set to be conical, and the discharge pipe 2 is connected to the sampling cylinder 1. A disinfection pipe 9 is sleeved on the discharge pipe 2. The disinfection pipe 9 is connected to the discharge pipe 2 by a clamp, and a valve 10 is provided on the disinfection pipe 9. A connecting sleeve 11 is sleeved on the outside of the push rod 8. The connecting sleeve 11 is located at one end of the sampling cylinder 1 and is engaged with the sampling cylinder 1. An extension tube 12 is fixedly provided at the end of the sampling cylinder 1 away from the connecting sleeve 11. A guide cavity is formed between the extension tube 12 and the piston disc 7.

[0024] When using the above structure, the staff holds the sampling tube 1 and pushes the push rod 8 to move. When the push rod 8 moves, it drives the piston column 3, piston disc 7, positioning block 4 and material collection tube 5 to move, so that the piston disc 7 can extend into the extension tube 12. Through the guide cavity between the piston disc 7 and the extension tube 12, the sample can flow between the piston column 3 and the piston disc 7. By rotating the push rod 8, the push rod 8 drives the positioning block 4, material collection tube 5 and material collection cavity 6 to rotate, so that the sample can flow into each material collection cavity 6 for storage, realizing the function of quantitative sampling of the sample.

[0025] Then, the push rod 8 is pulled back so that the sampling chamber 6 can be transported to the discharge port 2. By rotating the push rod 8, the samples in each sampling chamber 6 can be discharged through the discharge port 2 to ensure sampling accuracy. When the sampling chamber 6 and the sampling tube 5 are transported to the discharge port 2, the piston plate 7 can be located inside the sampling cylinder 1 to achieve the function of sealing the sampling cylinder 1 and preventing sample spillage.

[0026] Furthermore, during subsequent cleaning, an external water pipe is connected to the disinfection pipe 9, and then the valve 10 is opened to allow water to flow into the sampling cylinder 1 to rinse and disinfect the various structures inside the sampling cylinder 1, thus avoiding inaccurate sampling caused by repeated use.

[0027] Unlike existing technologies, this application discloses a sampling device for reusable sterilization in bioreactors. Through the flow guide cavity between the piston disc 7 and the extension tube 12, the sample flows between the piston column 3 and the piston disc 7. Rotating the push rod 8 causes the positioning block 4, the sampling tube 5, and the sampling chamber 6 to rotate, allowing the sample to flow into each sampling chamber 6 for storage, thus achieving quantitative sampling. Afterwards, pulling back the push rod 8 allows the sampling chamber 6 to be transported to the discharge port 2. Rotating the push rod 8 allows the sample in each sampling chamber 6 to be discharged through the discharge port 2, ensuring sampling accuracy. Furthermore, when the sampling chamber 6 and the sampling tube 5 are transported to the discharge port 2, the piston disc 7 is positioned inside the sampling cylinder 1 to seal it, preventing sample spillage.

[0028] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A sampling device for reusable sterilization in bioreactors, comprising a sampling cylinder (1), characterized in that: The sampling tube (1) is equipped with a sampling component; The sampling assembly includes a discharge port (2) located outside the sampling cylinder (1), a piston column (3) is provided inside the sampling cylinder (1), a positioning block (4) is provided at one end of the piston column (3), a material taking pipe (5) is fixedly provided on the outside of the positioning block (4), two material taking cavities (6) are opened on the material taking pipe (5), and a piston disc (7) is provided at the end of the positioning block (4) away from the piston column (3).

2. The sampling device for reusable sterilization in a bioreactor according to claim 1, characterized in that: A push rod (8) is provided in the middle of the piston rod (3), the positioning block (4) and the piston disc (7), and one end of the push rod (8) extends to the outside of the sampling cylinder (1).

3. The sampling device for reusable sterilization in a bioreactor according to claim 1, characterized in that: The vertical cross-sectional shape of the discharge pipe (2) is set to be conical, and the discharge pipe (2) is connected to the sampling cylinder (1).

4. A sampling device for reusable sterilization in a bioreactor according to claim 1, characterized in that: The discharge port (2) is fitted with a disinfection port (9), which is connected to the discharge port (2) by a clamp, and a valve (10) is provided on the disinfection port (9).

5. A sampling device for reusable sterilization in a bioreactor according to claim 2, characterized in that: The push rod (8) is fitted with a connecting sleeve (11) on its outer side. The connecting sleeve (11) is located at one end of the sampling cylinder (1) and is engaged with the sampling cylinder (1).

6. A sampling device for reusable sterilization in a bioreactor according to claim 5, characterized in that: An extension tube (12) is fixedly provided at the end of the sampling tube (1) away from the connecting sleeve (11), and a flow guide cavity is formed between the extension tube (12) and the piston disc (7).

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