Cell separation and enrichment device

Abstract

The utility model provides a cell separation and enrichment device, which relates to the technical field of cell separation and comprises a device body and a circular positioning disc, three circles of insertion holes which are diffused along the radial direction of the circular positioning disc are formed in the circular positioning disc in a penetrating manner, and cell carrying tubes penetrate through the insertion holes; a mounting ring is welded to the center of the top end of the circular positioning disc. The torsion driving frame is jointly composed of two L-shaped sliding shafts and a torsion ring welded between the head ends of the two L-shaped sliding shafts, and the two L-shaped sliding shafts are in penetrating sliding fit with the two positioning shaft rings in a spring pushing positioning mode; when the torsion driving frame is in a high-position state, the torsion ring is higher than the cell carrying tube; and a cover plate is rotationally mounted at the top end of the device body, and when the circular positioning disc and the torsion driving frame are both in a high-position state, the cover plate is in pushing contact with the torsion ring in the closing process. According to the utility model, an additional adaptive space for the torsion driving frame of the convex support on the device body is omitted, and the miniaturization of the separation and enrichment device is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of cell separation technology, and in particular to a cell separation and enrichment device. Background Technology

[0002] It is urgent to develop a cell separation and enrichment device that can efficiently and accurately separate and enrich lymphocytes and is perfectly compatible with the determination of lymphocyte micronucleus rate by cytology.

[0003] Existing separation and enrichment devices, in order to facilitate the holding and positioning of cell tubes of different lengths, often design the structure for batch positioning of the tubes as a height-adjustable sliding mechanism. This batch positioning structure also includes a locking mechanism for the tube body. However, the unlocking components of the locking mechanism are not well-designed; they are mostly positioned below the top of the tube. This causes the ring of tubes surrounding the unlocking component to obstruct and interfere with the manual operation of the unlocking component, making it difficult to drive the unlocking component and indirectly affecting the sliding adjustment efficiency of the batch positioning structure. Furthermore, although some unlocking components are designed to protrude from the ring of tubes, increasing the height of these protruding components requires a larger installation space, which to some extent increases the size of the device. Utility Model Content

[0004] In view of this, the present invention provides a cell separation and enrichment device to solve the problem of insufficient optimization design of the unlocking component.

[0005] The technical solution proposed by this utility model is: a cell separation and enrichment device, which is suitable for separating cell suspension in cell carrier tubes, specifically including a device body and a circular positioning disk. A cover plate is rotatably installed on the top of the device body, and a circular positioning disk is slidably arranged inside the device body.

[0006] The circular positioning disk has three concentric holes extending radially outwards, through which the cell carrier tube passes. A mounting ring is welded to the center of the top of the circular positioning disk, and a rotating ring is rotatably fitted onto the bottom side of the disk. Two sets of L-shaped support rods are symmetrically welded to the outer circumference of the rotating ring, and positioning collars are welded to the top of each set of L-shaped support rods. A torsion drive frame is mounted on both positioning collars. The torsion drive frame consists of two L-shaped sliding shafts and a torsion ring welded between the ends of the two L-shaped sliding shafts. The two L-shaped sliding shafts are slidably engaged with the two positioning collars by spring-driven positioning. When the torsion drive frame is in a high position, the torsion ring is higher than the cell carrier tube. A cover plate is rotatably mounted on the top of the device body. When both the circular positioning disk and the torsion drive frame are in a high position, the cover plate contacts the torsion ring during closing.

[0007] Furthermore, the device body is provided with an installation groove, and a centrifugal rotating seat is rotatably arranged in the bottom space of the installation groove. The circular positioning plate is located in the installation groove and placed above the centrifugal rotating seat.

[0008] A hexagonal positioning shaft is welded to the center of the top of the centrifugal rotating seat, and the central part of the circular positioning disk slides in conjunction with the hexagonal positioning shaft.

[0009] Furthermore, the centrifuge rotating seat has three concentric insertion holes that spread radially outwards, and the bottom section of the cell carrier tube is inserted into the insertion holes.

[0010] Furthermore, two L-shaped inserts, positioned by spring pushes, are symmetrically installed through the peripheral wall of the mounting ring.

[0011] The horizontal part of the L-shaped insert has a hexagonal structure, and a row of hexagonal positioning holes is opened along the height direction on the hexagonal positioning shaft. The first end of the horizontal part of the L-shaped insert is inserted into the row of hexagonal positioning holes.

[0012] Furthermore, the upright portion of the L-shaped insert has a circular structure and abuts against the outer periphery of the mounting ring, and a conical slot is formed between the upright portion of the L-shaped insert and the outer periphery of the mounting ring.

[0013] Furthermore, the rotating ring is located between two L-shaped inserts and a circular positioning disc. Two L-shaped support rods are symmetrically welded to the top of the rotating ring, and two opposing arc-shaped drive plates are welded to the ends of the two L-shaped support rods.

[0014] Furthermore, the first end of the arc-shaped drive plate has a conical tip structure. When the arc-shaped drive plate slides toward the L-shaped insert, the first end is inserted into the conical slot and abuts against the vertical part of the L-shaped insert.

[0015] The cell separation and enrichment device provided by this utility model has the following beneficial effects:

[0016] First, because the torsion drive frame can slide to a high position under the push of springs on its two L-shaped sliding shafts, and its upper torsion ring rises and protrudes into the space formed between the top parts of the cell carrier tubes surrounding it, higher than the cell carrier tubes, the circular positioning disk can be locked or unlocked directly and conveniently by torsionally rotating the torsion ring in the high position. Compared with the existing technology of directly using the rotating ring to drive the two L-shaped inserts internally and externally or setting the torsion drive frame in a low position between the top parts of the cell carrier tubes, this avoids the trouble of having to reach into the confined space formed by the top parts of the cell carrier tubes to grasp the drive rotating ring or torsion drive frame to lock or unlock the circular positioning disk. It avoids the obstruction and interference caused by the confined space to the movement of the drive rotating ring or torsion drive frame by the hand, which helps to indirectly improve the sliding adjustment efficiency of the circular positioning disk.

[0017] 2. When both the circular positioning plate and the torsion drive frame are in the high position, the cover plate contacts the torsion ring during the closing process and drives the entire torsion drive frame to move down along the two positioning rings. This can push and hide the idle and high-positioned torsion drive frame inside the mounting groove on the device body, preventing the torsion drive frame from remaining stationary and protruding from the mounting groove when idle. This eliminates the need to set additional adaptation space on the device body for the protruding torsion drive frame, which helps to reduce the volume of the separation and enrichment device to a certain extent and is conducive to the miniaturization of the separation and enrichment device. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings of the embodiments will be briefly described below.

[0019] The accompanying drawings described below are only related to some embodiments of the present invention and are not intended to limit the scope of the present invention.

[0020] In the attached diagram:

[0021] Figure 1 A schematic diagram of the overall structure of this utility model is shown;

[0022] Figure 2 A half-section internal structure diagram of the device body in this utility model is shown;

[0023] Figure 3 A schematic diagram of the centrifugal rotating seat and circular positioning disk in this utility model is shown;

[0024] Figure 4 A schematic diagram of the bottom structure of the centrifugal rotating seat and the circular positioning disk in this utility model is shown.

[0025] Figure 5 A schematic diagram showing the installation position of the transfer ring in this utility model is provided.

[0026] Figure 6 The diagram shows the disassembled state of the transfer ring and L-shaped insert of this utility model.

[0027] List of reference numerals in the attached diagram:

[0028] 1. Device body; 101. Cover plate;

[0029] 2. Centrifugal rotating seat; 201. Hexagonal positioning shaft;

[0030] 3. Circular positioning disc; 301. Mounting ring; 302. L-shaped insert rod; 303. Rotary ring; 3031. Positioning collar; 3032. Arc-shaped drive plate;

[0031] 4. Cell carrier tube;

[0032] 5. Torsional drive frame. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the described embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0034] Please refer to Figures 1 to 6 ;

[0035] Example 1:

[0036] This invention proposes a cell separation and enrichment device suitable for separating cell suspension in cell carrier tube 4. It includes a device body 1 and a circular positioning disk 3. A cover plate 101 is rotatably installed on the top of the device body 1, and a circular positioning disk 3 is slidably arranged inside the device body 1.

[0037] The circular positioning disk 3 has three concentric holes that radiate radially through it, and the cell carrier tube 4 passes through the holes. A mounting ring 301 is welded to the center of the top of the circular positioning disk 3. A rotating ring 303 is rotatably fitted on the bottom side of the circular positioning disk 3. Two sets of L-shaped support rods are symmetrically welded to the outer periphery of the rotating ring 303. A positioning shaft ring 3031 is welded to the top of each of the two sets of L-shaped support rods. A torsion drive frame 5 is mounted on both positioning shaft rings 3031. The torsion drive frame 5 is composed of two L-shaped sliding shafts and a torsion ring welded between the ends of the two L-shaped sliding shafts. The two L-shaped sliding shafts are slidably engaged with the two positioning shaft rings 3031 by spring-push positioning. When the torsion drive frame 5 is in a high position, the torsion ring is higher than the cell carrier tube 4. A cover plate 101 is rotatably mounted on the top of the device body 1.

[0038] Preferably, the device body 1 has an installation groove, and a centrifugal rotating seat 2 is rotatably arranged in the bottom space of the installation groove. The circular positioning disk 3 is located in the installation groove and placed above the centrifugal rotating seat 2. A hexagonal positioning shaft 201 is welded to the center of the top of the centrifugal rotating seat 2, and the central part of the circular positioning disk 3 slides with the hexagonal positioning shaft 201.

[0039] Preferably, the centrifuge rotating seat 2 has three concentric insertion holes that spread radially outwards, and the bottom section of the cell carrier tube 4 is inserted into the insertion holes.

[0040] Based on Example 1, Example 2:

[0041] Two L-shaped inserts 302 are symmetrically installed through the periphery of the mounting ring 301 and positioned by spring push. The horizontal part of the L-shaped insert 302 has a hexagonal structure. A row of hexagonal positioning holes is opened on the hexagonal positioning shaft 201 along the height direction. The first end of the horizontal part of the L-shaped insert 302 is inserted into the row of hexagonal positioning holes.

[0042] Preferably, the upright portion of the L-shaped insert 302 has a circular structure and abuts against the outer periphery of the mounting ring 301, and a conical slot is formed between the upright portion of the L-shaped insert 302 and the outer periphery of the mounting ring 301.

[0043] Preferably, the rotating ring 303 is located between two L-shaped inserts 302 and the circular positioning disk 3. Two L-shaped support rods are symmetrically welded to the top of the rotating ring 303, and two opposing arc-shaped drive plates 3032 are welded to the first ends of the two L-shaped support rods.

[0044] Preferably, the first end of the arc-shaped drive plate 3032 has a tapered tip structure. When the arc-shaped drive plate 3032 slides toward the L-shaped insert 302, the first end is inserted into the tapered slot and abuts against the vertical part of the L-shaped insert 302.

[0045] A centrifugal motor is installed on the bottom side of the inner side of the device body 1, and the centrifugal motor is connected to the centrifugal rotating seat 2 for transmission.

[0046] It is worth noting that the structure, installation method, and transmission connection structure between the centrifugal motor and the centrifugal rotating seat 2 are existing technologies for those skilled in the art who are engaged in equipment design and modification, and therefore will not be described in detail here.

[0047] The following provides a detailed and coherent explanation of the specific details, implementation steps, functions and interrelationships of the above-mentioned features, and their roles in realizing this invention:

[0048] The circular positioning disk 3 can position the cell carrier tube 4 in an upright position through its insertion hole, so as to prevent the cell carrier tube 4 from being thrown out of the insertion hole and broken due to impact when it is centrifuged by the centrifuge spinner 2 rotating at high speed to separate the cell suspension inside.

[0049] The circular positioning disk 3 can slide up and down along the hexagonal positioning axis 201 to adjust the distance between it and the centrifugal rotating seat 2, which is suitable for positioning and holding cell carrier tubes 4 of different lengths; when the two L-shaped insertion rods 302 are inserted into the hexagonal positioning holes at the corresponding heights on the hexagonal positioning axis 201, the cell carrier tube 4 can be positioned and held in the usage state after the height adjustment; through the arc-shaped driving plate 3032, the two arc-shaped driving plates 3032 follow the rotating ring 303 towards the two When the L-shaped insert 302 slides, it can push and drive the vertical parts of the two L-shaped inserts 302 to slide outward along the radial direction of the mounting ring 301, control the horizontal parts of the two L-shaped inserts 302 to separate from the hexagonal positioning holes, release the circular positioning disk 3, and when the rotating ring 303 rotates, it drives the two arc-shaped drive plates 3032 to separate from the vertical parts of the two L-shaped inserts 302, and the two L-shaped inserts 302 re-engage with the hexagonal positioning holes of the corresponding height, restoring the positioning of the circular positioning disk 3.

[0050] Because the two L-shaped sliding shafts of the torsion drive frame 5 are slidably connected to the two positioning rings 3031 on the rotating ring 303, the torsion drive frame 5 is connected to the rotating ring 303. The torsion drive frame 5 can torsionally drive the rotating ring 303 and control the two L-shaped inserts 302 to slide inward or outward to lock or unlock the circular positioning disk 3. Furthermore, because the torsion drive frame 5 can slide upward to a high position under the push of the springs on its two L-shaped sliding shafts, its upper torsion ring rises and protrudes into the space formed between the top parts of the cell carrier tubes 4 surrounding it, higher than the cell carrier tubes 4 (see reference). Figure 2 and Figure 3 Furthermore, by twisting the high-positioned torsion ring, the circular positioning disk 3 can be locked or unlocked directly and conveniently without obstruction. Compared with the existing technology of directly using the rotating ring 303 to drive the two L-shaped inserts 302 internally and externally, or setting the torsion drive frame 5 in a low position between the top parts of a ring of cell carrier tubes 4, this avoids the trouble of having to reach into the confined space formed by the top parts of a ring of cell carrier tubes 4 and grasp the driving rotating ring 303 or the torsion drive frame 5 to lock or unlock the circular positioning disk 3. It also avoids the obstruction and interference caused by the confined space to the hand driving the rotating ring 303 or the torsion drive frame 5, which helps to indirectly improve the sliding adjustment efficiency of the circular positioning disk 3.

[0051] When both the circular positioning disk 3 and the torsion drive frame 5 are in the high position, the cover plate 101 contacts the torsion ring during the closing process and drives the torsion drive frame 5 to move downward along the two positioning rings 3031 (refer to...). Figure 1This allows the idle and elevated torsion drive frame 5 to be pushed and hidden inside the mounting slot on the device body 1, preventing the torsion drive frame 5 from remaining stationary and protruding from the mounting slot when idle. This eliminates the need to provide additional adaptation space on the device body 1 for the protruding torsion drive frame 5, which helps to reduce the volume of the separation and enrichment device to a certain extent and is conducive to the miniaturization of the separation and enrichment device.

[0052] The working principle of this embodiment is as follows: In use, the cell carrier tubes 4 containing cell suspension are first inserted into the insertion holes of the centrifuge rotating seat 2 through the insertion holes on the circular positioning plate 3. Then, the cover plate 101 is closed and the motor is started. Driven by the motor, the centrifuge rotating seat 2 and the cell carrier tubes 4 rotate at high speed to centrifuge the cells in the cell suspension. After centrifugation for a specified time, the cells in the cell suspension are centrifuged and concentrated in the lower space of the cell carrier tubes 4, while the clear liquid separated from the cell suspension is suspended in the upper space of the cell carrier tubes 4. Finally, the cover plate 101 is opened, the cell carrier tubes 4 are taken out from the device body 1, and the clear liquid in the upper space of the cell carrier tubes 4 is poured out, thus completing the collection of the separated cells.

[0053] The following points should be noted in this article:

[0054] 1. The accompanying drawings of this utility model embodiment only involve the structure involved in this utility model embodiment; other structures can refer to general designs.

[0055] 2. Where there is no conflict, the embodiments of this utility model and the features in the embodiments can be combined with each other to obtain new embodiments.

[0056] The above are merely specific embodiments of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.

Claims

1. A cell separation and enrichment device, suitable for separating cell suspension in cell carrier tube (4), comprising a device body (1) and a circular positioning disk (3), wherein a cover plate (101) is rotatably installed on the top of the device body (1), and a circular positioning disk (3) is slidably arranged inside the device body (1); Its features are: The circular positioning disk (3) has three concentric insertion holes that radiate radially from it, through which the cell carrier tube (4) passes. A mounting ring (301) is welded to the center of the top of the circular positioning disk (3), and a rotating ring (303) is rotatably fitted onto the bottom side of the circular positioning disk (3). Two sets of L-shaped support rods are symmetrically welded to the outer periphery of the rotating ring (303), and positioning shaft rings (3031) are welded to the top of each of the two sets of L-shaped support rods. A torsion drive frame (5) is mounted on both positioning shaft rings (3031). The torsion drive frame (5) 5) It is composed of two L-shaped sliding shafts and a torsion ring welded between the two L-shaped sliding shafts. The two L-shaped sliding shafts are respectively connected to the two positioning shaft rings (3031) through the spring push positioning form. When the torsion drive frame (5) is in the high position, the torsion ring is higher than the cell carrier tube (4). The top of the device body (1) is rotatably installed with a cover plate (101). When both the circular positioning disk (3) and the torsion drive frame (5) are in the high position, the cover plate (101) is pushed and contacted by the torsion ring during the closing process.

2. The cell separation and enrichment device according to claim 1, characterized in that, The device body (1) is provided with an installation groove, and a centrifugal rotating seat (2) is rotatably arranged in the bottom space of the installation groove. The circular positioning plate (3) is located in the installation groove and is placed above the centrifugal rotating seat (2). A hexagonal positioning shaft (201) is welded to the center of the top of the centrifugal rotating seat (2), and the center of the circular positioning disk (3) slides with the hexagonal positioning shaft (201).

3. The cell separation and enrichment device according to claim 2, characterized in that, The centrifugal rotating seat (2) has three rings of insertion holes that spread radially outwards, and the bottom section of the cell carrier tube (4) is inserted into the insertion holes.

4. The cell separation and enrichment device according to claim 1, characterized in that, Two L-shaped inserts (302) are symmetrically installed through the peripheral wall of the mounting ring (301) and positioned by spring push; The horizontal part of the L-shaped insert (302) has a hexagonal structure, and a row of hexagonal positioning holes is opened on the hexagonal positioning shaft (201) along the height direction. The first end of the horizontal part of the L-shaped insert (302) is inserted into the row of hexagonal positioning holes.

5. The cell separation and enrichment device according to claim 4, characterized in that, The upright part of the L-shaped insert (302) has a circular structure and abuts against the outer periphery of the mounting ring (301). A conical slot is formed between the upright part of the L-shaped insert (302) and the outer periphery of the mounting ring (301).

6. The cell separation and enrichment device according to claim 4, characterized in that, The rotating ring (303) is located between two L-shaped inserts (302) and a circular positioning disk (3). Two L-shaped support rods are symmetrically welded to the top of the rotating ring (303), and two opposing arc-shaped drive plates (3032) are welded to the first ends of the two L-shaped support rods.

7. The cell separation and enrichment device according to claim 6, characterized in that, The first end of the arc-shaped drive plate (3032) has a cone-shaped structure. When the arc-shaped drive plate (3032) slides toward the L-shaped plug (302), the first end is inserted into the cone-shaped slot and abuts against the vertical part of the L-shaped plug (302).

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