Nucleic acid dye blue light photolysis experiment device

Abstract

The utility model provides a nucleic acid dye blue light photolysis experimental device, and belongs to the technical field of biological detection. The device comprises a door-shaped support, lamp panels and a cooling fan, the door-shaped support comprises a top plate and two side plates, the two side plates are arranged in parallel at intervals, the top plate is horizontally arranged at the tops of the side plates, mounting holes are formed in the top plate, the lamp panels are arranged below the top plate at intervals, lamp beads are arranged on the lamp panels, and the lamp beads face the mounting holes. And the cooling fan is arranged at the bottom of the lamp panel. By adopting the nucleic acid dye blue light photolysis experiment device provided by the embodiment of the utility model, the problem that the accuracy of an experiment result is relatively low due to relatively poor heat dissipation performance in the prior art can be solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological detection technical field, especially a nucleic acid dye blue light photolysis experimental device. BACKGROUND

[0002] PMA (propidium monoazide bromide) / EMA (ethidium monoazide bromide) and other nucleic acid pigments do not have membrane permeability and can selectively enter viruses / bacteria with incomplete membranes, and damaged viral capsid envelopes are one of the reasons for the loss of infectivity of viruses, and the incomplete cell wall and cell membrane of bacteria will cause the death of bacteria due to the outflow of membrane contents. PMA / EMA is an azide compound, which can crosslink with nucleic acids under corresponding excitation wavelength light irradiation conditions, and after blue light photolysis, the photoreactive azide group on PMA / EMA is converted into a highly reactive nitrene radical, which is inserted between the C-H bonds of nucleic acids, thereby causing permanent nucleic acid modification. This modification process makes nucleic acids insoluble and is removed in subsequent nucleic acid extraction, and inhibits the nucleic acid amplification process during detection. When PMA / EMA selectively enters non-infectious viruses / bacteria and crosslinks with their nucleic acids under blue light, the nucleic acids of non-infectious viruses / bacteria are removed or the amplification is inhibited, and then various detection methods are used to distinguish infectious and non-infectious viruses / bacteria.

[0003] Based on the above characteristics, PMA / EMA can be used to distinguish infectious and non-infectious viruses / bacteria, and an important link is blue light photolysis. In the prior art, the blue light photolysis experimental device is usually bulky, and a halogen lamp is usually used to irradiate the sample. The photolysis is time-consuming and laborious, it is difficult to meet the requirements of the precise excitation wavelength of PMA / EMA, and the sample is heated, which affects the crosslinking of PMA / EMA and nucleic acids.

[0004] The blue light photolysis experimental device in the prior art uses a halogen lamp for long-time light irradiation, which causes the sample to be heated during the photolysis experiment, thereby affecting the accuracy of the experimental results. UTILITY MODEL CONTENT

[0005] The utility model embodiment provides a nucleic acid dye blue light photolysis experimental device, can solve the problem that the accuracy of experimental results is low due to poor heat dissipation in the prior art. The technical scheme is as follows:

[0006] A nucleic acid dye blue light photolysis experimental device, comprising: a door type support, a lamp panel and a cooling fan,

[0007] The door type support comprises a top plate and two side plates, the two side plates are arranged in parallel and at intervals, the top plate is horizontally arranged at the top of the two side plates, mounting holes are formed in the top plate, a lamp plate is arranged below the top plate, lamp beads are arranged on the lamp plate, the lamp beads are arranged towards the mounting holes, and a heat dissipation fan is arranged at the bottom of the lamp plate.

[0008] Optionally, the mounting holes are arranged in a plurality of mounting holes, and the plurality of mounting holes are arranged in a rectangular array on the top plate.

[0009] Optionally, the lamp beads are arranged in a plurality of lamp beads, and the plurality of lamp beads are arranged in a rectangular array on the lamp plate.

[0010] Optionally, the door type support further comprises an aluminum foil, and the aluminum foil covers the opening formed by the top plate and the side plates.

[0011] Optionally, the opposite sides of the two side plates are provided with reflective materials.

[0012] Optionally, the lamp plate is provided with a lamp holder arranged on the heat dissipation fan.

[0013] Optionally, the lamp plate and the lamp holder are detachably connected.

[0014] Optionally, the door type support further comprises a safe flame-retardant power supply box and a plug, the safe flame-retardant power supply box is internally provided with a transformer, the plug is electrically connected to the lamp beads through the transformer, and the plug is electrically connected to the heat dissipation fan.

[0015] Optionally, the safe flame-retardant power supply box is arranged in two safe flame-retardant power supply boxes, and the two safe flame-retardant power supply boxes are arranged on the two sides of the lamp plate, respectively.

[0016] Optionally, the door type support further comprises a switch, and the switch is electrically connected to the plug.

[0017] The technical scheme provided by the embodiment of the utility model has at least the following beneficial effects:

[0018] The nucleic acid dye blue light photolysis experimental device provided by the embodiment of the utility model puts the sample tube into the mounting hole, so that the sample enters the space below the top plate, and the lamp beads on the lamp plate irradiate the sample in the sample tube with blue light, the fan below the lamp plate can dissipate heat for the lamp plate, thereby reducing the influence of the heat generated by the lamp plate on the sample. The blue light photolysis experiment is carried out by using the structure, and the structure is simple, light and has good ventilation and heat dissipation performance, and the problem that the poor heat dissipation in the prior art leads to low accuracy of experimental results can be effectively solved. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical scheme in the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without any creative effort.

[0020] Figure 1 is the overall structure schematic diagram of the device provided by the embodiment of the present application;

[0021] Figure 2 is the overall structure schematic diagram of the device provided by the embodiment of the present application after installing the sample;

[0022] Figure 3 is the overall structure schematic diagram of the device provided by the embodiment of the present application after removing the aluminum foil paper;

[0023] Figure 4 is the overall structure cross-sectional schematic diagram of the device provided by the embodiment of the present application;

[0024] Figure 5 is the overall structure schematic diagram of the device provided by the embodiment of the present application after removing the door type support;

[0025] Figure 6 is the top view structure schematic diagram of the device provided by the embodiment of the present application. Figure 5

[0026] In the figure: 1-door type support; 11-top plate; 111-mounting hole; 12-side plate; 2-lamp plate; 21-lamp bead; 22-lamp holder; 3-heat dissipation fan; 4-aluminum foil paper; 5-safety flame-retardant power supply box; 51-transformer; 6-plug; 7-switch. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the present application more clear, the following will further describe the embodiments of the present application in combination with the drawings.

[0028] Figure 1 is the overall structure schematic diagram of the device provided by the embodiment of the present application; Figure 2 is the overall structure schematic diagram of the device provided by the embodiment of the present application after installing the sample; Figure 3 is the overall structure schematic diagram of the device provided by the embodiment of the present application after removing the aluminum foil paper; Figure 4 is the overall structure cross-sectional schematic diagram of the device provided by the embodiment of the present application; Figure 5 is the overall structure schematic diagram of the device provided by the embodiment of the present application after removing the door type support; Figure 6 is the top view structure schematic diagram of the device provided by the embodiment of the present application. Figure 5 As shown in the drawings,​Figures 1 to 6 The nucleic acid dye blue light photolysis experiment device shown comprises a door-shaped support 1, a lamp plate 2 and a heat dissipation fan 3, the door-shaped support 1 comprises a top plate 11 and two side plates 12, the two side plates 12 are arranged in parallel and at intervals, the top plate 11 is horizontally arranged at the top of the two side plates 12, the top plate 11 is provided with a mounting hole 111, the lamp plate 2 is arranged below the top plate 11 at intervals, the lamp plate 2 is provided with lamp beads 21, the lamp beads 21 are arranged towards the mounting hole 111, and the heat dissipation fan 3 is arranged at the bottom of the lamp plate 2.

[0029] Exemplarily, in the embodiment of the utility model, the lamp beads 21 adopt LED lamp beads, the lamp plate 2 adopts super-high heat-conducting aluminum substrate, the wavelength of the lamp beads 21 is 465-470nm, the electric power is 40W, and the light power is 40mW / cm 2To ensure the efficiency of photolysis of blue light. The overall length of the door-shaped bracket 1 is 20 cm, the width is 17 cm, the height is 30 cm, and the thickness is 5 cm. It is made of acrylic material and has high transparency, which will not absorb light sources and affect the photolysis effect. The sample is in duplicate, 200 μL each, placed in a 1.5 mL enzyme-free centrifuge tube. One of them is added with PMA / EMA dye under light protection. After short vortex centrifugation, dark incubation is carried out to allow PMA / EMA dye molecules to enter non-infectious viruses / bacteria. The other group of samples is used as a control without adding PMA / EMA, and DEPC water can be added as a control. The rest of the conditions are the same. Then, the sample is transferred to the installation hole 111 under light protection. The top plate 11 can be provided with a plurality of installation holes 111. If the sample is not too much, it is placed in the installation hole 111 in the middle position of the top plate 11. The cover of the sample tube is just clamped on the installation hole 111, and the sample liquid is at the bottom of the sample tube. The light plate 2 is irradiated from bottom to top without blocking the light, ensuring sufficient photolysis. The heat dissipation fan 3 is used to dissipate heat from the light plate 2 to avoid experimental errors caused by heating the sample. After photolysis, the sample nucleic acid is extracted and purified, and the nucleic acid is stored at -80°C. Then, one-step TaqMan fluorescent quantitative PCR method is used to distinguish infectious and non-infectious viruses / bacteria. The control group without adding PMA / EMA in the sample is subjected to ordinary qPCR process. Ordinary qPCR cannot distinguish nucleic acid from infectious viruses / bacteria, so the detection result indicates the total nucleic acid content in the sample, which includes infectious and non-infectious viruses / bacteria. The experimental group with PMA / EMA added to the sample is subjected to PMA / EMA-qPCR process. Because the nucleic acid of non-infectious viruses / bacteria is removed or the amplification is inhibited, the detection result indicates the nucleic acid content of infectious viruses / bacteria in the sample. By comparison, if the sample contains non-infectious viruses / bacteria, the cycle threshold (Ct) in the quantitative result is larger than that of the group without adding PMA / EMA. The detection method of nucleic acid is not limited to the method mentioned in this example. Methods such as PCR, LAMP, ddPCR, RPA, and RAA can be used to distinguish infectious and non-infectious viruses / bacteria. In this embodiment, the door-shaped bracket 1 is made of acrylic material, which is more portable and has lower manufacturing cost compared to the metal shell in the prior art. In the prior art, traditional halogen lamps have high power, are easy to heat, and consume a lot of electricity. In the process of long-time heating, fire is easy to occur, which poses a hidden danger to personnel, laboratory safety, and environmental safety. Most LED lamps on the market can provide a single light source, but the power is small and the light intensity is low, which prolongs the detection time and reduces the detection sensitivity. The lamp bead 21 in this embodiment uses a blue light high-power LED lamp bead, which can photolyze more quickly and more easily reach the requirement of PMA / EMA precise excitation wavelength, thereby improving the experimental efficiency.

[0030] The utility model discloses an experimental device of blue light photolysis of nucleic acid dye is provided, through putting sample tube on mounting hole 211, make sample enter the space below top plate 11, and adopt the lamp pearl 21 on the lamp board 2 to the sample in sample tube carries out blue light irradiation, the fan 3 below the lamp board 2 can be the heat dissipation for lamp board 2, thereby reducing the influence of lamp board 2 heating to sample.

[0031] Optionally, the mounting hole 111 is provided with a plurality of mounting holes 111, and the plurality of mounting holes 111 are arranged in a rectangular array on the top plate 11.

[0032] Exemplarily, in the utility model embodiment, twenty-five mounting holes 111 are arranged in five rows and five columns, which are used for placing 1.5ml sample tubes 10 containing samples for photolysis. By arranging a plurality of mounting holes 111, a plurality of sample tubes can be placed for simultaneous experiment, which can improve the experimental efficiency of blue light photolysis experiment. When the number of samples to be detected is small, the sample tubes can be placed in the mounting holes 111 close to the middle part, so that the blue light received by the samples is more uniform, and the photolysis efficiency is improved.

[0033] Optionally, the lamp pearl 21 is provided with a plurality of lamp pearls 21, and the plurality of lamp pearls 21 are arranged in a rectangular array on the lamp board 2.

[0034] Exemplarily, in the utility model embodiment, 96 lamp pearls 21 are arranged in eight rows and twelve columns. By arranging a plurality of lamp pearls 21, the blue light received by the samples can be more uniform, so that the samples in each sample tube can receive sufficient blue light for decomposition, which further improves the photolysis efficiency and ensures the accuracy of the experimental results.

[0035] Optionally, the utility model further includes an aluminum foil 4, and the aluminum foil 4 is arranged at the opening formed by the top plate 11 and the side plate 12.

[0036] Exemplarily, in the utility model embodiment, the aluminum foil 4 has a light-reflecting surface and a non-light-reflecting surface. The light-reflecting surface of the aluminum foil 4 is arranged towards the inside of the door-shaped support 1. By additionally arranging the aluminum foil 4, the light beams generated by the lamp pearls 21 can be reflected, so that the light beams are concentrated in the inside of the door-shaped support 1, and thus the samples in the inside of the door-shaped support 1 can receive more sufficient blue light, which further improves the photolysis efficiency. After the sample tubes are placed on the mounting holes 111, a layer of aluminum foil 4 can also be laid on the top plate 11 to prevent the light beams from leaking out of the top plate, which further improves the photolysis efficiency.

[0037] Optionally, the opposite side of the two side plates 12 is provided with a light-reflecting material.

[0038] Exemplarily, in the embodiment of the utility model, the reflective material can be reflective stickers, or a reflective coating is brushed on the surface of the side plate 12, by setting the reflective material, the light generated by the lamp bead 21 will not be absorbed by the side plate 12, the light beam meets the reflective material and is reflected, and the sample is further irradiated, so that the photolysis efficiency is further improved.

[0039] Optionally, the lamp panel 2 is provided with a lamp holder 22 arranged on the heat dissipation fan 3.

[0040] Exemplarily, in the embodiment of the utility model, by setting the lamp holder 22, the lamp panel 2 can be arranged in parallel and spaced apart at the bottom of the top plate 11, which provides support for the lamp panel 2, and the lamp panel 2 can be arranged above the heat dissipation fan 3, so that the heat dissipation fan 3 can continuously cool the lamp panel 2, and the lamp holder 22 can be made of 304 stainless steel, which has the characteristics of corrosion resistance and no pollution. By setting the lamp holder 22, the structural stability of the device is improved.

[0041] Optionally, the lamp panel 2 and the lamp holder 22 are detachably connected.

[0042] Exemplarily, in the embodiment of the utility model, bolts are arranged at the four corners of the lamp panel 2, the top of the lamp panel 2 and the lamp holder 22 are installed through the bolts, the lamp panel 2 and the lamp holder 22 can be quickly disassembled or installed through this structure, and a nylon gasket can be added between the bolt and the lamp panel 2 for insulation to prevent short circuit. When the lamp panel 2 is worn out after a long time of use, the lamp panel 2 can be disassembled and a new lamp panel 2 can be installed for use, thereby prolonging the service life of the device.

[0043] Optionally, it also includes a safe flame-retardant power supply box 5 and a plug 6, the safe flame-retardant power supply box 5 is internally provided with a transformer 51, the plug 6 is electrically connected with the lamp bead 21 through the transformer 51, and the plug 6 is electrically connected with the heat dissipation fan 3.

[0044] Exemplarily, in the embodiment of the utility model, the plug 6 is used for plugging an external socket, which is convenient for directly connecting common 220V power supply, the transformer 51 is used for converting 220V power supply into low-voltage power supply for the lamp bead 21, and the safe flame-retardant power supply box 5 is also provided with a flame-retardant material to prevent fire caused by excessive voltage, by setting this structure, the operation is simple, it is convenient to connect with a common circuit, it is convenient to use the device for blue light experiment in various scenes, and the convenience of the device is improved.

[0045] Optionally, the safe flame-retardant power supply box 5 is provided with two, and the two safe flame-retardant power supply boxes 5 are arranged on the two sides of the lamp panel 2.

[0046] Exemplarily, in the embodiment of the utility model, two safe flame -retardant power boxes 5 are arranged, namely two transformers 51 are arranged, on the one hand, can share the current, guarantee power, on the other hand, one transformer 51 is powered to odd row lamp pearl 21, another transformer 51 is powered to even row lamp pearl 21, by setting this structure, when one transformer 51 fails, another transformer 51 still can work and is powered to lamp pearl 21, still can carry out photolysis experiment under reducing half quantity lamp pearl 21, to this improves the practicality of the device.

[0047] Optionally, further comprising a switch 7, the switch 7 is electrically connected with the plug 6.

[0048] Exemplarily, in the embodiment of the utility model, by setting switch 7, the plug 6 can be plugged with external socket for a long time, by controlling switch 7, the starting and stopping of the device can be carried out, and the plug 6 does not need to be frequently plugged and unplugged, to improve the operation convenience of the device.

[0049] Unless otherwise defined, technical terms or scientific terms used herein should be understood as having the common meaning in the field of the utility model. The "first", "second" and similar words used in the utility model patent application specification and claims do not represent any order, quantity or importance, but are used to distinguish different components. Similarly, "one" or "a" and similar words do not represent quantity limitation, but represent the existence of at least one. "Include" or "contain" and similar words mean that the elements or objects appearing before "include" or "contain" cover the elements or objects listed after "include" or "contain" and their equivalents, and do not exclude other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connection, but can include electrical connection, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to represent relative positional relationship, when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0050] The above is only optional embodiment of the utility model, and does not limit the utility model, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. A device for blue light photolysis experiments of nucleic acid dyes, characterized in that, The utility model relates to a kind of LED street lamp, including: Door type support (1), lamp panel (2) and heat dissipation fan (3), The door type support (1) includes top plate (11) and two side plates (12), two the side plate (12) is arranged in parallel spacing, the top plate (11) is horizontally arranged at the top of two the side plate (12), mounting hole (111) is opened in the top plate (11), the lamp panel (2) is arranged below the top plate (11), lamp pearl (21) is provided on the lamp panel (2), the lamp pearl (21) is arranged towards the mounting hole (111), the heat dissipation fan (3) is arranged at the bottom of the lamp panel (2).

2. The device for blue light photolysis experiment of nucleic acid dyes according to claim 1, characterized in that, The mounting hole (111) is provided with multiple, multiple the mounting hole (111) is distributed in rectangular array on the top plate (11).

3. The device for blue light photolysis experiment of nucleic acid dyes according to claim 1, characterized in that, The lamp pearl (21) is provided with multiple, multiple the lamp pearl (21) is distributed in rectangular array on the lamp panel (2).

4. The device for blue light photolysis experiment of nucleic acid dyes according to claim 1, characterized in that, It further includes aluminum foil paper (4), and the aluminum foil paper (4) is covered at the opening formed by the top plate (11) and the side plate (12).

5. The apparatus of claim 1, wherein the apparatus is configured to perform a blue light photolysis experiment on a nucleic acid dye. Two the side plate (12) is provided with reflective material on opposite side.

6. The device of claim 1, wherein the device is configured to perform a blue light photolysis experiment on a nucleic acid dye. The lamp panel (2) bottom is provided with lamp stand (22), and the lamp stand (22) is erected on the heat dissipation fan (3).

7. The device of claim 6, wherein the device is configured to perform a blue light photolysis experiment on a nucleic acid dye. The lamp panel (2) and the lamp stand (22) are detachably connected.

8. The device of claim 1, wherein the device is configured to perform a blue light photolysis experiment on a nucleic acid dye. It further includes safety flame-retardant power supply box (5) and plug (6), the safety flame-retardant power supply box (5) is provided with transformer (51) inside, the plug (6) is electrically connected with the lamp pearl (21) by the transformer (51), and the plug (6) is electrically connected with the heat dissipation fan (3).

9. The apparatus of claim 8, wherein the apparatus further comprises a blue light source. The safety flame-retardant power supply box (5) is provided with two, and two the safety flame-retardant power supply box (5) is arranged at the two sides of the lamp panel (2) respectively.

10. The apparatus of claim 8, wherein the apparatus is configured to perform a blue light photolysis experiment on a nucleic acid dye. It further includes switch (7), and the switch (7) is electrically connected with the plug (6).

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