Auxiliary device for nucleic acid extraction

Abstract

The utility model relates to an auxiliary device for nucleic acid extraction, which comprises a mounting plate, a first connecting plate and a second connecting plate, the plurality of magnetic attraction brackets are provided with magnetic blocks, and each magnetic attraction bracket can be arranged on the first plate surface in a manner of moving up and down; the heating supports are provided with temperature control parts, each temperature control part is provided with a containing groove with an opening in the top, and the heating supports can be arranged on the second plate face in an up-down moving mode; the different heating brackets are moved upwards, so that the sample treatment pipes located above the heating brackets are inserted into the corresponding accommodating grooves, and the to-be-tested samples in the sample treatment pipes are heated through the temperature control parts; the magnetic block is moved to a position close to or in contact with the temperature control part by moving different magnetic attraction brackets upwards, and the magnetic beads in the sample treatment pipe are attracted to the pipe wall through the magnetic block. The nucleic acid extraction device solves the technical problem that the detection effect is influenced by less released nucleic acid due to insufficient nucleic acid extraction.

Description

Technical Field

[0001] This invention relates to the field of molecular diagnostic technology, and in particular to an auxiliary device for nucleic acid extraction. Background Technology

[0002] Molecular diagnostics refers to technologies that use DNA and RNA as diagnostic materials and employ molecular biology techniques to diagnose human conditions and diseases by detecting the presence, defects, or abnormal expression of genes. Its basic principle is to detect changes in the structure, quantity, and expression function of DNA or RNA to determine whether the subject has abnormal changes at the gene level. This is of great significance for the prevention, prediction, diagnosis, treatment, and prognosis of diseases. In other words, all methodologies and techniques based on molecular biology fall under the category of molecular diagnostics, such as polymerase chain reaction (PCR) and gene sequencing.

[0003] To complete molecular diagnostic testing on a sample, nucleic acid extraction and nucleic acid amplification steps are required. Nucleic acid extraction refers to the process of releasing nucleic acid (DNA or RNA) from cells or viruses and removing impurities other than nucleic acid by using various reagents and instruments. Nucleic acid amplification refers to the replication and amplification of the extracted nucleic acid using PCR technology.

[0004] The instrument required for nucleic acid extraction from the sample to be tested is a nucleic acid extractor. However, existing nucleic acid extractors sometimes fail to extract enough nucleic acid, resulting in poor extraction efficiency and a smaller amount of released nucleic acid, which affects the detection results.

[0005] Therefore, this utility model proposes an auxiliary device for nucleic acid extraction to overcome the shortcomings of the prior art. Utility Model Content

[0006] The purpose of this invention is to provide an auxiliary device for nucleic acid extraction, which can improve the nucleic acid extraction effect, make the nucleic acid extraction of the sample to be tested more complete, and enable the sample to be tested to be extracted on demand, thereby speeding up the detection speed and improving the detection efficiency of the corresponding nucleic acid.

[0007] The objective of this utility model can be achieved by the following solutions:

[0008] This utility model provides an auxiliary device for nucleic acid extraction, used to heat and magnetically attract multiple sample processing tubes containing samples to be tested, arranged side-by-side above it. The auxiliary device for nucleic acid extraction includes:

[0009] Mounting plate, the mounting plate having opposing first and second surfaces;

[0010] Multiple magnetic brackets, each having a magnetic block, are mounted on the first plate surface and can be moved up and down.

[0011] Multiple heating brackets, each heating bracket having a temperature control device, each temperature control device having a receiving groove with a top opening, and each heating bracket being movable up and down on the second plate surface and positioned opposite to each of the magnetic brackets;

[0012] By moving the different heating brackets upward, the sample processing tube located above them is inserted into the corresponding receiving slot, and the sample to be tested in the sample processing tube is heated by the corresponding temperature control.

[0013] By moving the different magnetic supports upwards, the magnetic block is moved to a position close to or in contact with the temperature control opposite it, and the magnetic beads in the sample processing tube are attracted to the tube wall by the magnetic block.

[0014] In a preferred embodiment of this invention, the auxiliary device for nucleic acid extraction further includes:

[0015] Multiple vertically extending first guide rails, each first guide rail corresponding to one of the multiple magnetic brackets, each first guide rail being arranged side by side and spaced apart on the first plate surface, and each magnetic bracket being movable up and down on its corresponding first guide rail;

[0016] Multiple vertically extending second guide rails are provided, each second guide rail corresponding to one of the multiple heating brackets. Each second guide rail is disposed on the second plate surface and is positioned opposite to each of the first guide rails. Each heating bracket is movable up and down on its corresponding second guide rail.

[0017] In a preferred embodiment of the present invention, the temperature control device has a side opening on the side facing the magnetic support that communicates with the receiving groove, so that the magnetic block on the magnetic support can contact the tube wall of the sample processing tube in the corresponding receiving groove through the side opening.

[0018] In a preferred embodiment of the present invention, a heating element and / or a heat-insulating element are provided at the bottom of the temperature control device.

[0019] In a preferred embodiment of the present invention, a first slider that can slide along the first guide rail is provided on the first guide rail, and the magnetic bracket is connected to the first slider.

[0020] The auxiliary device for nucleic acid extraction further includes a first motor and a vertically arranged first guide shaft. The output shaft of the first motor is vertically upward and connected to the bottom end of the first guide shaft. A first connecting block is provided between the first guide shaft and the magnetic support. One end of the first connecting block is connected to the magnetic support, and the other end of the first connecting block has a first threaded hole. The first connecting block is sleeved on the first guide shaft through the first threaded hole and screwed into the external thread on the first guide shaft.

[0021] In a preferred embodiment of this utility model, there are multiple first motors, and each of the multiple first motors corresponds to one of the multiple magnetic brackets, so as to drive the corresponding magnetic brackets to move up and down respectively.

[0022] In a preferred embodiment of the present invention, a first lower optocoupler switch and a first upper optocoupler switch are provided on one side of the first guide rail, and a first optocoupler baffle is provided on the first connecting block. The first optocoupler baffle is used to move up and down with the first connecting block and trigger the first lower optocoupler switch to limit the downward movement of the magnetic bracket, or to trigger the first upper optocoupler switch to limit the upward movement of the magnetic bracket.

[0023] In a preferred embodiment of this utility model, a second slider that can slide along the second guide rail is provided on the second guide rail, and the heating bracket is connected to the second slider;

[0024] The auxiliary device for nucleic acid extraction also includes a second motor and a vertically arranged second guide shaft. The output shaft of the second motor is vertically upward and connected to the bottom end of the second guide shaft. A second connecting block is provided between the second guide shaft and the heating bracket. One end of the second connecting block is connected to the heating bracket, and the other end of the second connecting block has a second threaded hole. The second connecting block is sleeved on the second guide shaft through the second threaded hole and screwed into the external thread on the second guide shaft.

[0025] In a preferred embodiment of this utility model, there are multiple second motors, and each of the multiple second motors corresponds to one of the multiple heating brackets, so as to drive the corresponding heating brackets to move up and down respectively.

[0026] In a preferred embodiment of the present invention, a second lower optocoupler switch and a second upper optocoupler switch are provided on one side of the second guide rail, and a second optocoupler baffle is provided on the second connecting block. The second optocoupler baffle is used to move up and down with the second connecting block and trigger the second lower optocoupler switch to limit the downward movement of the heating bracket, or to trigger the second upper optocoupler switch to limit the upward movement of the heating bracket.

[0027] As described above, the features and advantages of the auxiliary device for nucleic acid extraction of this utility model are:

[0028] Multiple magnetic holders and multiple heating holders are respectively positioned opposite each other on the first and second surfaces of the mounting plate. During use, different heating holders can be moved upwards to allow the sample processing tubes located above the corresponding heating holders to be inserted into the receiving slots of the corresponding heating holders. The temperature control on the heating holders heats the sample to be tested in the sample processing tubes, enabling the sample processing tubes to reach the set target temperature. This creates a suitable temperature environment for nucleic acid extraction, accelerates the lysis and release of nucleic acids from the sample to be tested, and ensures more thorough and efficient extraction of nucleic acids from the sample to be tested, thereby improving the nucleic acid detection efficiency. By moving different magnetic holders upwards, the magnetic blocks are moved to positions close to or in contact with their corresponding temperature control, and the magnetic blocks on the magnetic holders attract the magnetic beads in the sample processing tubes to the tube wall (the magnetic beads have released nucleic acids adsorbed on them), thus improving the nucleic acid extraction effect.

[0029] In addition, since the positions of multiple magnetic holders and multiple heating holders can be controlled independently without affecting each other, the samples to be tested can be tested at any time, realizing the on-demand collection of samples, accelerating the detection speed and improving the efficiency of nucleic acid detection. Attached Figure Description

[0030] The following figures are intended only to illustrate and explain the present invention and do not limit the scope of the present invention. Wherein:

[0031] Figure 1 This is a perspective view of the auxiliary device for nucleic acid extraction according to this utility model;

[0032] Figure 2 This is one of the structural schematic diagrams of the auxiliary device for nucleic acid extraction of this utility model in a disassembled state;

[0033] Figure 3 This is the second structural schematic diagram of the auxiliary device for nucleic acid extraction of this utility model in a disassembled state;

[0034] Figure 4 This is the third structural diagram of the auxiliary device for nucleic acid extraction of this utility model in a partially disassembled state.

[0035] The reference numerals in the accompanying drawings of this utility model are:

[0036] 1. Mounting plate; 101. First panel;

[0037] 102. Second panel; 2. First guide rail;

[0038] 201. First slider; 3. Magnetic support;

[0039] 301. Magnetic block; 4. Second guide rail;

[0040] 401. Second slider; 5. Heating bracket;

[0041] 501, Temperature control unit; 5011, Receiving slot;

[0042] 502. Heating element; 503. Insulation element;

[0043] 6. First motor; 7. First guide shaft;

[0044] 8. First connecting block; 9. Second motor;

[0045] 10. Second guide shaft; 11. Second connecting block;

[0046] 12. Base plate; 13. First column;

[0047] 14. First lower optocoupler switch; 15. First upper optocoupler switch;

[0048] 16. First optocoupler baffle; 17. First lower circuit board;

[0049] 18. First upper circuit board; 19. Second column;

[0050] 20. Second lower circuit board; 21. Second upper circuit board;

[0051] 22. Second upper optocoupler switch. Detailed Implementation

[0052] The technical solution of this utility model will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that these embodiments are only used to illustrate this utility model and are not intended to limit the scope of this utility model. After reading this utility model, any modifications of this utility model in various equivalent forms by those skilled in the art will fall within the scope defined by the appended claims.

[0053] It should be noted that when an element is referred to as being "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0054] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0055] like Figures 1 to 4 As shown, this utility model provides an auxiliary device for nucleic acid extraction, which is used to heat and magnetically attract multiple sample processing tubes (not shown) arranged side by side above it, each containing a sample to be tested. The auxiliary device for nucleic acid extraction includes a base plate 12, a mounting plate 1, multiple magnetic supports 3 and multiple heating supports 5. The base plate 12 is arranged horizontally, and the mounting plate 1 is arranged vertically on the base plate 12. The bottom of the mounting plate 1 is fixedly connected to the top surface of the base plate 12. The mounting plate 1 has two opposing vertical surfaces, namely a first surface 101 and a second surface 102. Each magnetic bracket 3 is movably mounted on the first surface 101, and each magnetic bracket 3 has a magnetic block 301. Each heating bracket 5 is movably mounted on the second surface 102 and is positioned opposite to each magnetic bracket 3. By moving different heating brackets 5 upwards, the sample processing tube located above the corresponding heating bracket 5 is inserted into the receiving groove 5011 of the corresponding heating bracket 5, and the sample to be tested in the sample processing tube is heated by the temperature control 501 on the corresponding heating bracket 5. By moving different magnetic brackets 3 upwards, the magnetic block 301 on the corresponding magnetic bracket 3 is moved to a position close to or in contact with the temperature control 501 on the opposite heating bracket 5, and the magnetic bead in the sample processing tube is attracted to the tube wall by the magnetic block 301 on the corresponding magnetic bracket 3.

[0056] It should be noted that the heating brackets 5 described above are movably mounted on the second plate 102 and are positioned opposite to each magnetic bracket 3. This can be understood as multiple heating brackets 5 and multiple magnetic brackets 3 being mounted opposite each other on two opposite plates of the mounting plate 1. The position of each heating bracket 5 in the width direction of the mounting plate 1 is the same as the position of each magnetic bracket 3 in the width direction of the mounting plate 1, so that one heating bracket 5 can be aligned and cooperate with one magnetic bracket 3 to achieve heating and magnetic treatment of the sample to be tested in a sample processing tube.

[0057] In this invention, multiple magnetic support brackets 3 and multiple heating brackets 5 are respectively and oppositely arranged on the first plate surface 101 and the second plate surface 102 of the mounting plate 1. During use, different heating brackets 5 can be moved upwards so that the sample processing tube located above the corresponding heating bracket 5 can be inserted into the receiving groove 5011 of the corresponding heating bracket 5. The sample to be tested in the sample processing tube is heated by the temperature control 501 on the heating bracket 5 so that the sample processing tube can reach the set target temperature, creating a suitable temperature environment for nucleic acid extraction, accelerating the lysis and nucleic acid release of the sample to be tested, ensuring more complete and efficient extraction of nucleic acid from the sample to be tested, and improving the nucleic acid detection efficiency. By moving different magnetic support brackets 3 upwards, the magnetic blocks 301 on the magnetic support bracket 3 can be moved to a position close to or in contact with the temperature control 501 on the heating bracket 5 opposite to it. The magnetic blocks 301 on the magnetic support bracket 3 adsorb the magnetic beads in the sample processing tube onto the tube wall (the magnetic beads adsorb the released nucleic acid), thereby improving the nucleic acid extraction effect.

[0058] In this invention, since the vertical movement of multiple magnetic support 3 and multiple heating support 5 can be controlled individually (i.e., the different magnetic support 3, the different heating support 5, and the magnetic support 3 and the heating support 5 are all controlled individually), they do not affect each other. Therefore, the sample to be tested can be tested at any time, realizing the sample to be tested can be picked up as soon as it arrives, speeding up the detection speed and improving the efficiency of nucleic acid detection.

[0059] In one optional embodiment of this utility model, such as Figures 2 to 4 As shown, the auxiliary device for nucleic acid extraction also includes multiple vertically extending first guide rails 2 and multiple vertically extending second guide rails 4. Each first guide rail 2 corresponds to a plurality of magnetic support brackets 3. The first guide rails 2 are arranged side by side and spaced apart on the first plate surface 101. Each magnetic support bracket 3 is movably mounted on its corresponding first guide rail 2. The first guide rail 2 provides a sliding guide for the magnetic support bracket 3, ensuring that the magnetic support bracket 3 can move back and forth vertically to achieve smooth position adjustment. Each second guide rail 4 corresponds to a plurality of heating brackets 5. Each second guide rail 4 is arranged on the second plate surface 102 and is positioned opposite to each first guide rail 2. Each heating bracket 5 is movably mounted on its corresponding second guide rail 4. The second guide rail 4 provides a sliding guide for the heating bracket 5, ensuring that the heating bracket 5 can move back and forth vertically to achieve smooth position adjustment.

[0060] Furthermore, such as Figure 2 As shown, at least one first slider 201 is provided on the first guide rail 2, which can slide along the first guide rail 2. The first slider 201 is slidably connected to the first guide rail 2, and the magnetic bracket 3 is connected to the first slider 201.

[0061] Furthermore, such as Figure 3 As shown, at least one second slider 401 is provided on the second guide rail 4, which can slide along the second guide rail 4. The second slider 401 is slidably connected to the second guide rail 4, and the heating bracket 5 is connected to the second slider 401.

[0062] In an optional embodiment of this utility model, the temperature control unit 501 has a side opening communicating with the receiving groove 5011 on the side facing the magnetic support 3. The width of the side opening is smaller than the diameter of the sample processing tube. Therefore, after the sample processing tube is inserted into the receiving groove 5011, the sample processing tube will not tilt due to the side opening, nor will it tip over and fall to the side of the side opening. Because of the side opening, when the magnetic block 301 on the magnetic support 3 moves to the same height as the temperature control unit 501 on the heating support 5, the magnetic block 301 can directly contact the wall of the sample processing tube in the receiving groove 5011, thereby ensuring that all the magnetic beads inside the sample processing tube are attracted to the wall of the sample processing tube. The magnetic block 301 can be, but is not limited to, a block magnet.

[0063] In one optional embodiment of this utility model, such as Figure 3 As shown, a heating element 502 is provided at the bottom of the temperature control unit 501. The heating element 502 is in direct contact with the bottom of the temperature control unit 501, and heats the temperature control unit 501 through the heating element 502, thereby ensuring that the sample processing tube in the receiving tank 5011 can reach the preset target temperature, so as to ensure the efficient and smooth extraction of nucleic acid. The target temperature can be set by the operator to ensure that the sample to be tested can be fully lysed and release nucleic acid. There is no limitation on the target temperature.

[0064] Furthermore, the heating element 502 may be, but is not limited to, a Peltier.

[0065] In one optional embodiment of this utility model, such as Figure 3 As shown, a heat preservation element 503 is also provided at the bottom of the temperature control unit 501. The heat preservation element 503 is connected to the heating element 502 and the heating bracket 5 respectively. The heat preservation element 503 can slow down the heat loss in the temperature control unit 501, play a role in keeping the sample processing tube warm, and prolong the time that the sample processing tube is in a suitable temperature environment, thereby ensuring the smooth progress of nucleic acid extraction.

[0066] Furthermore, the insulation element 503 may be, but is not limited to, a foam layer.

[0067] In one optional embodiment of this utility model, such as Figures 1 to 4As shown, the auxiliary device for nucleic acid extraction also includes a first motor 6 and a vertically arranged first guide shaft 7. The first motor 6 is fixedly mounted on the top surface of the base plate 12, and its output shaft is vertically upward. The top end of the output shaft is connected to the bottom end of the first guide shaft 7. A first connecting block 8 is provided between the first guide shaft 7 and the magnetic support 3. One end of the first connecting block 8 is connected to the magnetic support 3, and the other end of the first connecting block 8 has a first threaded hole. The first connecting block 8 is sleeved on the first guide shaft 7 through the first threaded hole and screwed into the external thread on the first guide shaft 7. The output shaft of the first motor 6 drives the first guide shaft 7 to rotate, thereby driving the first connecting block 8 to move up and down along the first guide shaft 7. Since the first connecting block 8 is connected to the magnetic support 3, the first connecting block 8 will drive the magnetic support 3 to move up and down. Thus, the first motor 6 provides the driving force for the magnetic support 3 to move up and down, thereby controlling the magnetic support 3 to slide along the first guide rail 2.

[0068] In this utility model, there are multiple first motors 6, and each of the multiple first motors 6 corresponds to a multiple magnetic brackets 3, so as to drive the corresponding magnetic brackets 3 to move up and down, thereby realizing individual control of different magnetic brackets 3.

[0069] In one optional embodiment of this utility model, such as Figure 1 and Figure 4 As shown, a first lower optocoupler switch 14 and a first upper optocoupler switch 15 are provided on one side of the first guide rail 2, and a first optocoupler baffle 16 is provided on the first connecting block 8. The first optocoupler baffle 16 is used to move up and down with the first connecting block 8 and trigger the first lower optocoupler switch 14 to limit the downward movement of the magnetic bracket 3, or trigger the first upper optocoupler switch 15 to limit the upward movement of the magnetic bracket 3.

[0070] Furthermore, such as Figure 1 and Figure 4 As shown, the number of first lower optocoupler switches 14 and the number of first upper optocoupler switches 15 are both multiple and equal. The multiple first lower optocoupler switches 14 correspond one-to-one with the multiple magnetic brackets 3, and the multiple first upper optocoupler switches 15 also correspond one-to-one with the multiple magnetic brackets 3, thereby limiting the upward and downward positions of different magnetic brackets 3 respectively.

[0071] Specifically, such as Figure 1 and Figure 4As shown, multiple first columns 13 are provided on one side of the first guide rail 2. The bottom end of the first column 13 is connected to the top surface of the base plate 12. A first lower circuit board 17 and a first upper circuit board 18 are respectively provided at the lower and upper parts of the first column 13. Multiple first lower optocoupler switches 14 are spaced apart and evenly distributed on the first lower circuit board 17, and multiple first upper optocoupler switches 15 are spaced apart and evenly distributed on the first upper circuit board 18. During operation, the first connecting block 8 will drive the first optocoupler baffle 16 to move up and down. When the first optocoupler baffle 16 contacts the first lower optocoupler switch 14, the first lower optocoupler switch 14 receives a signal and transmits it to the controller. The controller then controls the corresponding first motor 6 to stop the magnetic suction bracket 3 from moving down. When the first optocoupler baffle 16 contacts the first upper optocoupler switch 15, the first upper optocoupler switch 15 receives a signal and transmits it to the controller. The controller then controls the corresponding first motor 6 to stop the magnetic suction bracket 3 from moving up.

[0072] In one optional embodiment of this utility model, such as Figures 1 to 4 As shown, the auxiliary device for nucleic acid extraction also includes a second motor 9 and a vertically arranged second guide shaft 10. The second motor 9 is fixedly mounted on the top surface of the base plate 12, and its output shaft is vertically upward. The top end of the output shaft is connected to the bottom end of the second guide shaft 10. A second connecting block 11 is provided between the second guide shaft 10 and the heating support 5. One end of the second connecting block 11 is connected to the heating support 5, and the other end of the second connecting block 11 has a second threaded hole. The second connecting block 11 is sleeved on the second guide shaft 10 through the second threaded hole and screwed into the external thread on the second guide shaft 10. The output shaft of the second motor 9 drives the second guide shaft 10 to rotate, thereby driving the second connecting block 11 to move up and down along the second guide shaft 10. Since the second connecting block 11 is connected to the heating support 5, it drives the heating support 5 to move up and down. Thus, the second motor 9 provides the driving force for the heating support 5 to move up and down, thereby controlling the heating support 5 to slide along the second guide rail 4.

[0073] In this invention, there are multiple second motors 9, and each of the multiple second motors 9 corresponds to a multiple heating bracket 5, so as to drive the corresponding heating bracket 5 to move up and down, thereby realizing individual control of different heating brackets 5.

[0074] In one optional embodiment of this utility model, such as Figure 1 and Figure 4As shown, a second lower optocoupler switch (not shown) and a second upper optocoupler switch 22 are provided on one side of the second guide rail 4. A second optocoupler baffle (not shown) is provided on the second connecting block 11. The second optocoupler baffle is used to move up and down with the second connecting block 11 and trigger the second lower optocoupler switch to limit the downward movement of the heating bracket 5, or to trigger the second upper optocoupler switch 22 to limit the upward movement of the heating bracket 5.

[0075] Furthermore, such as Figure 1 and Figure 4 As shown, the number of the second lower optocoupler switches and the number of the second upper optocoupler switches 22 are both multiple and equal. The multiple second lower optocoupler switches correspond one-to-one with the multiple heating brackets 5, and the multiple second upper optocoupler switches 22 also correspond one-to-one with the multiple heating brackets 5, thereby limiting the upward and downward positions of different heating brackets 5 respectively.

[0076] Specifically, such as Figure 1 and Figure 4 As shown, multiple second columns 19 are provided on one side of the second guide rail 4. The bottom end of the second column 19 is connected to the top surface of the base plate 12. A second lower circuit board 20 and a second upper circuit board 21 are respectively provided at the lower and upper parts of the second column 19. Multiple second lower optocoupler switches are spaced apart and evenly distributed on the second lower circuit board 20, and multiple second upper optocoupler switches 22 are spaced apart and evenly distributed on the second upper circuit board 21. During operation, the second connecting block 11 will drive the second optocoupler baffle to move up and down. When the second optocoupler baffle contacts the second lower optocoupler switch, the second lower optocoupler switch receives a signal and transmits it to the controller. The controller then controls the corresponding second motor 9 to stop the heating bracket 5 from moving down. When the second optocoupler baffle contacts the second upper optocoupler switch 22, the second upper optocoupler switch 22 receives a signal and transmits it to the controller. The controller then controls the corresponding second motor 9 to stop the heating bracket 5 from moving up.

[0077] In this invention, multiple sample processing tubes are fixed above multiple heating supports 5 using fasteners. Each sample processing tube is vertically aligned with a receiving groove 5011 on one of the heating supports 5, ensuring that the sample processing tubes can be smoothly inserted into the corresponding receiving groove 5011 during the upward movement of the heating supports 5. During the detection process, the sample to be tested, lysis buffer, magnetic beads, washing solution, and other substances are sequentially added to the sample processing tube according to the nucleic acid extraction steps, thereby performing nucleic acid extraction processing on the sample to be tested. The fasteners can be of any structure, as long as they can fix the sample processing tubes in a preset position and ensure their stability; the specific structure of the fasteners is not limited here. Alternatively, a multi-degree-of-freedom robotic arm or other gripper structure can be used to clamp and fix the sample processing tubes.

[0078] The working process of the auxiliary device for nucleic acid extraction of this utility model is as follows:

[0079] According to the actual testing needs, the corresponding heating bracket 5 is controlled to move upward. When the sample processing tube located above the heating bracket 5 is inserted into the receiving groove 5011 on the heating bracket 5 (the sample processing tube is fixed in the receiving groove 5011 and does not move), the heating element 502 on the corresponding heating bracket 5 can be controlled to heat the sample processing tube (this can be the lysis and release of nucleic acid stage). The temperature inside the sample processing tube rises, thereby accelerating the lysis of the sample to be tested and speeding up the release of nucleic acid. In addition, heating the sample processing tube can also make the sample to be tested and the reagents in the processing tube fully mixed, making the release of nucleic acid from the sample to be tested more thorough on the basis of accelerating the release of nucleic acid.

[0080] When the magnetic block 301 on the magnetic support 3 moves to contact the wall of the sample processing tube on the corresponding heating support 5, the magnetic block 301 on the magnetic support 3 can adsorb the magnetic beads with adsorbed nucleic acid in the sample processing tube onto the wall of the sample processing tube (this can be the washing stage for the released nucleic acid), thus facilitating the discharge of waste liquid in the sample processing tube, leaving only the magnetic beads with adsorbed nucleic acid in the sample processing tube. Then, new reagents (such as washing solution) can be injected into the sample processing tube. After the new reagent is injected, the magnetic block 301 can be moved by the up-and-down reciprocating movement of the magnetic support 3, and then the magnetic block 301 can move the adsorbed magnetic beads along the wall of the sample processing tube, so that the magnetic beads and the reagent in the sample processing tube are more evenly mixed, thereby improving the nucleic acid extraction effect.

[0081] The features and advantages of the auxiliary device for nucleic acid extraction of this invention are as follows:

[0082] This auxiliary device for nucleic acid extraction can, on the one hand, enable the immediate extraction of samples without the need for centralized processing of multiple samples, thereby improving the efficiency of nucleic acid extraction; on the other hand, it can enhance the effect of nucleic acid extraction by controlling independent temperature control and magnetic suction, making the nucleic acid extraction of the samples more complete and accelerating the detection speed.

[0083] It should be noted that in the description of this application, the terms "first," "second," etc., are used only for descriptive purposes and to distinguish similar objects; there is no order between them, nor should they be construed as indicating or implying relative importance. Furthermore, in the description of this application, unless otherwise stated, "multiple" means two or more.

[0084] The various embodiments described in this specification are presented in a progressive manner. The same or similar parts between the embodiments can be referred to each other. Each embodiment focuses on the differences from other embodiments.

[0085] The above are merely several embodiments of this utility model. Although the embodiments disclosed in this utility model are as described above, the content is only for the purpose of facilitating understanding of this utility model and is not intended to limit this utility model. Any equivalent changes and modifications made by those skilled in the art without departing from the concept and principles of this utility model should fall within the protection scope of this utility model.

Claims

1. An auxiliary device for nucleic acid extraction, used for heating and magnetically attracting multiple sample processing tubes, each containing a sample to be tested, arranged side-by-side above it, characterized in that... The auxiliary device for nucleic acid extraction includes: Mounting plate, the mounting plate having opposing first and second surfaces; Multiple magnetic brackets, each having a magnetic block, are mounted on the first plate surface and can be moved up and down. Multiple heating brackets, each heating bracket having a temperature control device, each temperature control device having a receiving groove with a top opening, and each heating bracket being movable up and down on the second plate surface and positioned opposite to each of the magnetic brackets; By moving the different heating brackets upward, the sample processing tube located above them is inserted into the corresponding receiving slot, and the sample to be tested in the sample processing tube is heated by the corresponding temperature control. By moving the different magnetic supports upwards, the magnetic block is moved to a position close to or in contact with the temperature control opposite it, and the magnetic beads in the sample processing tube are attracted to the tube wall by the magnetic block.

2. The auxiliary device for nucleic acid extraction as described in claim 1, characterized in that, The auxiliary device for nucleic acid extraction also includes: Multiple vertically extending first guide rails, each first guide rail corresponding to one of the multiple magnetic brackets, each first guide rail being arranged side by side and spaced apart on the first plate surface, and each magnetic bracket being movable up and down on its corresponding first guide rail; Multiple vertically extending second guide rails are provided, each second guide rail corresponding to one of the multiple heating brackets. Each second guide rail is disposed on the second plate surface and is positioned opposite to each of the first guide rails. Each heating bracket is movable up and down on its corresponding second guide rail.

3. The auxiliary device for nucleic acid extraction as described in claim 1, characterized in that, The temperature control has a side opening facing the magnetic support that communicates with the receiving groove, so that the magnetic block on the magnetic support can contact the wall of the sample processing tube in the corresponding receiving groove through the side opening.

4. The auxiliary device for nucleic acid extraction as described in claim 1, characterized in that, The bottom of the temperature control device is provided with a heating element and / or a heat preservation element.

5. The auxiliary device for nucleic acid extraction as described in claim 2, characterized in that, The first guide rail is provided with a first slider that can slide along the first guide rail, and the magnetic bracket is connected to the first slider; The auxiliary device for nucleic acid extraction further includes a first motor and a vertically arranged first guide shaft. The output shaft of the first motor is vertically upward and connected to the bottom end of the first guide shaft. A first connecting block is provided between the first guide shaft and the magnetic support. One end of the first connecting block is connected to the magnetic support, and the other end of the first connecting block has a first threaded hole. The first connecting block is sleeved on the first guide shaft through the first threaded hole and screwed into the external thread on the first guide shaft.

6. The auxiliary device for nucleic acid extraction as described in claim 5, characterized in that, There are multiple first motors, and each of the multiple first motors corresponds to one of the multiple magnetic brackets, so as to drive the corresponding magnetic bracket to move up and down.

7. The auxiliary device for nucleic acid extraction as described in claim 5, characterized in that, A first lower optocoupler switch and a first upper optocoupler switch are provided on one side of the first guide rail. A first optocoupler baffle is provided on the first connecting block. The first optocoupler baffle is used to move up and down with the first connecting block and trigger the first lower optocoupler switch to limit the downward movement of the magnetic bracket, or to trigger the first upper optocoupler switch to limit the upward movement of the magnetic bracket.

8. The auxiliary device for nucleic acid extraction as described in claim 2, characterized in that, The second guide rail is provided with a second slider that can slide along the second guide rail, and the heating bracket is connected to the second slider; The auxiliary device for nucleic acid extraction also includes a second motor and a vertically arranged second guide shaft. The output shaft of the second motor is vertically upward and connected to the bottom end of the second guide shaft. A second connecting block is provided between the second guide shaft and the heating bracket. One end of the second connecting block is connected to the heating bracket, and the other end of the second connecting block has a second threaded hole. The second connecting block is sleeved on the second guide shaft through the second threaded hole and screwed into the external thread on the second guide shaft.

9. The auxiliary device for nucleic acid extraction as described in claim 8, characterized in that, There are multiple second motors, and each of the multiple second motors corresponds to one of the multiple heating brackets, so as to drive the corresponding heating bracket to move up and down.

10. The auxiliary device for nucleic acid extraction as described in claim 8, characterized in that, A second lower optocoupler switch and a second upper optocoupler switch are provided on one side of the second guide rail. A second optocoupler baffle is provided on the second connecting block. The second optocoupler baffle is used to move up and down with the second connecting block and trigger the second lower optocoupler switch to limit the downward movement of the heating bracket, or to trigger the second upper optocoupler switch to limit the upward movement of the heating bracket.

Images