Double-layer cover eight-connection pipe

The double-layer cap design of the inner and outer cap components solves the problem of the difficulty in opening the PCR eight-tube tube twice, enabling convenient reagent addition and sealing, and ensuring the safety and efficiency of the operation.

CN223963511UActive Publication Date: 2026-03-03TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-03-20
Publication Date
2026-03-03

AI Technical Summary

Technical Problem

The existing PCR eight-tube strips are difficult to open again after being capped, which makes reagent preparation difficult and poses a risk of splashing. The operation is time-consuming and laborious, and there is also a risk of sample contamination and infection.

Method used

A double-layer cap eight-tube assembly was designed, including an inner cap assembly and an outer cap assembly. The inner cap assembly consists of a long plate, a limiting ring, and a connecting ring, with a thin film inside the connecting ring. The outer cap assembly consists of a connecting plate, a tube cap, a limiting post, and a sealing gasket. The double sealing structure ensures airtightness and allows for easy opening of the outer cap to add reagents.

Benefits of technology

It achieves convenient reagent addition and sealing, avoids reagent spillage, reduces operational difficulty and infection risk, and improves operational efficiency and safety.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a double-layer cover eight-connection pipe which comprises an eight-connection pipe assembly, an inner cover assembly is installed on the top of the eight-connection pipe assembly in an inserted mode, and an outer cover assembly used for covering the eight-connection pipe assembly is installed on the upper surface of the inner cover assembly. The eight-connection-pipe assembly comprises a pipe body and a connecting cylinder, the connecting cylinder is installed at the top of the pipe body in a threaded mode, and a protruding block is installed at the end of the connecting cylinder in a surrounding mode. The inner cover assembly comprises a long plate, a limiting ring sleeve and a connecting ring, the outer cover assembly comprises a connecting plate, the limiting column and the limiting ring sleeve are installed in an inserted mode, and the sealing ring pad is installed in the connecting ring in an inserted mode. Double protection of the inner cover assembly and the outer cover assembly is designed through the double-layer cover, the sealing performance of the eight-connection tube is ensured, the double-layer tube cover is covered after reagent preparation is completed, the film is arranged in the middle of the inner-layer connecting ring, and when extracted nucleic acid needs to be added, only the outer-layer tube cover needs to be opened; then the sample adding gun punctures the thin film in the middle of the inner-layer connecting ring to be mixed with the reagent, and the outer-layer tube cover is covered, so that the operation is convenient.
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Description

Technical Field

[0001] The embodiments of this utility model belong to the field of medical auxiliary equipment technology, and more specifically, relate to a double-layered eight-section tube. Background Technology

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be viewed as a special form of DNA replication outside of living organisms. The biggest feature of PCR is its ability to significantly increase minute amounts of DNA. PCR technology is used in nucleic acid detection. After manually collecting samples, the sample solution is added to a PCR tube, followed by the system reagents. The PCR tube is then capped and placed in a PCR device for amplification and quantitative fluorescence detection. Because daily molecular biology tests involve a large number of samples and the volume of a single PCR tube is small, commercially available PCR tubes are usually arranged in an eight-tube array to achieve high-throughput detection and easy transfer. This means that eight PCR tubes are connected in a row, and correspondingly, the caps of the eight-tube PCR tubes are also connected in a row.

[0003] The PCR eight-tubes currently in use are single-use, fully sealed eight-tubes. Once the cap is tightened, it is difficult to open them a second time. If nucleic acid or system reagents need to be added, it is difficult to open the PCR eight-tubes again. Forcibly opening them a second time can easily cause the PCR reaction solution to splash out.

[0004] Manual capping is used to press the tube caps onto the PCR eight-tube strips, but this method requires highly skilled staff, is time-consuming, labor-intensive, inefficient, and carries the risk of sample contamination and infection. Utility Model Content

[0005] In view of the above-mentioned defects or improvement needs of the existing technology, the present invention provides a double-layer cover eight-pipe assembly, including an eight-pipe assembly, an inner cover assembly is inserted and installed on the top of the eight-pipe assembly, and an outer cover assembly for covering the eight-pipe assembly is installed on the upper surface of the inner cover assembly.

[0006] The eight-pipe assembly includes a pipe body and a connecting cylinder. The connecting cylinder is threaded onto the top of the pipe body, and a protrusion is mounted around the end of the connecting cylinder.

[0007] The inner cover assembly includes a long plate, a limiting ring sleeve, and a connecting ring. The limiting ring sleeve is installed at both ends of the upper surface of the limiting ring sleeve. The connecting ring is evenly installed on the surface of the long plate. The surface of the connecting ring is surrounded by slots. A thin film is installed inside the connecting ring. The protrusion is inserted into the inside of the slot.

[0008] The outer cover assembly includes a connecting plate, a pipe cover groove, a pipe cover, a limiting post, and a sealing ring gasket. The limiting post is fixedly installed at both ends of the lower surface of the connecting plate and is inserted into the limiting ring. Several sets of pipe cover grooves are formed on the upper surface of the connecting plate. The pipe cover is hinged to one side of the connecting plate. Several sets of sealing ring gaskets are fixedly installed on the bottom surface of the connecting plate and are inserted into the inside of the connecting ring.

[0009] Preferably, the top of the tube is provided with an end cylinder, the outside of the end cylinder is provided with external threads, the inside of the connecting cylinder is provided with internal threads, and the protrusion and the end cylinder are detachably installed.

[0010] Preferably, the connecting plate has an opening groove in the middle, a cover ring is fixedly installed on the upper part of the opening groove, corner grooves are opened on both sides of the end of the pipe cover groove, and a hand-turning groove is opened in the middle of the end of the pipe cover groove.

[0011] Preferably, a connecting piece and a turning piece are provided on one side of the pipe cover, and both the connecting piece and the turning piece are fixedly connected to the connecting plate. A pipe cover cylinder is provided in the middle of the pipe cover, and the pipe cover cylinder is movably inserted into the inside of the connecting piece.

[0012] Preferably, the opening of the end tube is smaller than the opening of the tube body, and the diameter of the connecting tube is equal to the diameter of the tube body.

[0013] Preferably, the size of the tube cap cylinder matches the size of the opening slot.

[0014] Preferably, the connecting piece and the transition piece are made of a malleable material.

[0015] In summary, compared with the prior art, the above-described technical solution conceived by this utility model can achieve the following beneficial effects:

[0016] (1) This utility model ensures the sealing performance of the eight-tube by using a double-layer cap design with an inner cap assembly and an outer cap assembly for double protection. After the reagent is prepared, the double-layer cap is put on. The inner connecting ring has a thin film in the middle. When the extracted nucleic acid needs to be added, only the outer cap needs to be opened. Then, the pipette is used to puncture the thin film in the middle of the inner connecting ring to mix with the reagent. After putting on the outer cap, PCR amplification can be performed. Since the inner layer does not need to be broken open, the reagent can be avoided from splashing out. The cap of the outer cap assembly can be opened or closed easily, which is convenient for operation.

[0017] (2) The tube body of the eight-tube assembly of this utility model is used to contain PCR reaction solution and other samples. The limiting ring is installed at both ends of the upper surface of the long plate and is used to insert and cooperate with the limiting column in the outer cover assembly to realize the initial connection between the inner cover assembly and the outer cover assembly. The connecting ring is evenly installed on the surface of the long plate. Each connecting ring is equipped with a thin film to provide additional sealing performance. The limiting column is used to insert and cooperate with the limiting ring in the inner cover assembly to realize the stable connection between the outer cover assembly and the inner cover assembly. The sealing gasket is fixedly installed on the bottom surface of the connecting plate and inserted into the inside of the connecting ring to provide additional sealing performance and ensure that the reaction solution will not leak due to poor sealing during the PCR reaction. Attached Figure Description

[0018] Figure 1 This is a structural diagram of the present invention;

[0019] Figure 2 This is a diagram showing the overall structure of this utility model.

[0020] Figure 3 This is a structural diagram of a single eight-tube assembly of this utility model;

[0021] Figure 4 This is a structural diagram of the inner cover assembly of this utility model;

[0022] Figure 5 This is a bottom structural diagram of the inner cover assembly of this utility model;

[0023] Figure 6 This is a structural diagram of the outer cover assembly of this utility model.

[0024] In all the accompanying drawings, the same reference numerals denote the same technical features, specifically: 1-Eight-pipe assembly; 11-Pipe body; 111-End cylinder; 112-External thread; 12-Connecting cylinder; 121-Internal thread; 122-Protrusion; 2-Inner cover assembly; 21-Long plate; 22-Restricting ring; 23-Connecting ring; 231-Slot; 232-Membrane; 3-Outer cover assembly; 31-Connecting plate; 32-Pipe cover groove; 321-Opening groove; 322-Cover protrusion; 323-Corner groove; 324-Hand-flip groove; 33-Pipe cover; 331-Connecting piece; 332-Turn piece; 333-Pipe cover cylinder; 34-Restricting post; 35-Sealing gasket. Detailed Implementation

[0025] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0026] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0027] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0028] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the present utility model and are not intended to limit the present utility model. Furthermore, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0029] A double-layered cap eight-joint pipe, including as follows Figures 1-6 As shown, in this embodiment of the utility model, an eight-tube assembly 1 is included, an inner cover assembly 2 is inserted and installed on the top of the eight-tube assembly 1, and an outer cover assembly 3 for covering the eight-tube assembly 1 is installed on the upper surface of the inner cover assembly 2.

[0030] The eight-pipe assembly 1 includes a pipe body 11 and a connecting sleeve 12. The connecting sleeve 12 is threaded onto the top of the pipe body 11, and a protrusion 122 is mounted around the end of the connecting sleeve 12.

[0031] The inner cover assembly 2 includes a long plate 21, a limiting ring 22 and a connecting ring 23. The limiting ring 22 is installed at both ends of the upper surface of the limiting ring 22. The connecting ring 23 is evenly installed on the surface of the long plate 21. The surface of the connecting ring 23 is surrounded by a slot 231. A thin film 232 is installed inside the connecting ring 23. The protrusion 122 is inserted into the inside of the slot 231.

[0032] The outer cover assembly 3 includes a connecting plate 31, a pipe cover groove 32, a pipe cover 33, a limiting post 34, and a sealing ring gasket 35. The limiting post 34 is fixedly installed at both ends of the lower surface of the connecting plate 31. The limiting post 34 is inserted into the limiting ring sleeve 22. Several sets of pipe cover grooves 32 are opened on the upper surface of the connecting plate 31. The pipe cover 33 is hinged to one side of the connecting plate 31. Several sets of sealing ring gaskets 35 are fixedly installed on the bottom surface of the connecting plate 31. The sealing ring gaskets 35 are inserted into the inside of the connecting ring 23.

[0033] In this embodiment, the tube body 11 of the eight-tube assembly 1 is used to contain samples such as PCR reaction solution. The connecting tube 12 is threaded onto the top of the tube body 11 for connection with the inner cover assembly 2. The end of the connecting tube 12 is surrounded by protrusions 122, which are used to engage with the slots 231 in the inner cover assembly 2 for initial fixation. The long plate 21 of the inner cover assembly 2 serves as the basic structure of the inner cover assembly 2, supporting and connecting other components. The limiting rings 22 are installed at both ends of the upper surface of the long plate 21 for engagement with the limiting posts 34 in the outer cover assembly 3. This achieves the initial connection between the inner cover assembly 2 and the outer cover assembly 3. Connecting rings 23 are evenly installed on the surface of the long plate 21. Each connecting ring 23 has a diaphragm 232 installed inside to provide additional sealing performance. Slots 231 are formed around the surface of the connecting rings 23 for insertion and engagement with the protrusions 122 at the end of the connecting cylinder 12, further securing the connection between the inner cover assembly 2 and the eight-tube assembly 1. The connecting plate 31 of the outer cover assembly 3 serves as the basic structure of the outer cover assembly 3. Limiting posts 34 are fixedly installed at both ends of the lower surface of the connecting plate 31 for engagement with the limiting rings 2 in the inner cover assembly 2. 2. Insertion and connection: The upper surface of the connecting plate 31 has multiple sets of tube cap grooves 32 for accommodating and fixing tube caps 33. The tube caps 33 are hinged to one side of the connecting plate 31, allowing for easy opening and closing to facilitate the addition or removal of samples. The limiting post 34 is used to insert and cooperate with the limiting ring 22 in the inner cap assembly 2, achieving a stable connection between the outer cap assembly 3 and the inner cap assembly 2. The sealing ring gasket 35 is fixedly installed on the bottom surface of the connecting plate 31 and inserted into the inside of the connecting ring 23 to provide additional sealing performance, ensuring that the PCR reaction will not be interrupted due to poor sealing. To prevent liquid leakage, the double-layer cap design, with inner cap assembly 2 and outer cap assembly 3 providing dual protection, ensures the sealing performance of the eight-tube strip. After reagent preparation, the double-layer cap 33 is closed. The inner connecting ring 23 has a thin film 232 in the middle. When the extracted nucleic acid needs to be added, simply open the outer cap 33, then use a pipette to puncture the thin film 232 in the middle of the inner connecting ring 23 to mix with the reagent. After closing the outer cap 33, PCR amplification can be performed. Since the inner layer does not need to be broken open, reagent splashing can be avoided. The cap 33 of the outer cap assembly 3 can be easily opened or closed for convenient operation.

[0034] like Figure 3 As shown, specifically, the top of the tube body 11 is provided with an end cylinder 111, the outside of the end cylinder 111 is provided with an external thread 112, the inside of the connecting cylinder 12 is provided with an internal thread 121, and the protrusion 122 and the end cylinder 111 are detachably installed.

[0035] In this embodiment, the top of the tube body 11 is equipped with an end cylinder 111. The end cylinder 111 is designed with an external thread 112 on the outside, and the connecting cylinder 12 is designed with an internal thread 121 on the inside. The internal thread 121 matches the external thread 112, so that the connecting cylinder 12 can be screwed and fixed on the end cylinder 111 of the tube body 11. The threaded connection is simple and effective, and is often used in occasions where frequent disassembly or reinstallation is required. The protrusion 122 further strengthens the connection between the connecting cylinder 12 and the tube body 11.

[0036] Specifically, the connecting plate 31 has an opening groove 321 in the middle, a cover ring 322 is fixedly installed on the upper part of the opening groove 321, corner grooves 323 are opened on both sides of the end of the pipe cover groove 32, and a hand-flipping groove 324 is opened in the middle of the end of the pipe cover groove 32.

[0037] In this embodiment, an opening groove 321 is formed in the middle of the connecting plate 31. A cover ring 322 is fixedly installed on the upper part of the opening groove 321. The cover ring 322 is a ring structure used to increase the strength and rigidity of the opening groove 321. Triangular corner grooves 323 are formed on both sides of the end of the tube cover groove 32, providing operating space when flipping the cover. A hand-flipping groove 324 is formed in the middle of the end of the tube cover groove 32, so that the fingers can flip the tube cover 33.

[0038] like Figure 6 As shown, specifically, a connecting piece 331 and a turning piece 332 are provided on one side of the pipe cover 33. Both the connecting piece 331 and the turning piece 332 are fixedly connected to the connecting plate 31. A pipe cover cylinder 333 is provided in the middle of the pipe cover 33. The pipe cover cylinder 333 can be movably inserted into the inside of the connecting piece 331.

[0039] In this embodiment, a connecting piece 331 and a turning piece 332 are provided on one side of the pipe cover 33. These two components are typically used to connect the pipe cover 33 to the connecting plate 31. The connecting piece 331 is an L-shaped component used to provide connection area and stability. The turning piece 332 is a component that extends from the connecting piece 331 and changes direction, used to adapt to specific installation spaces or provide additional connection points. Both the connecting piece 331 and the turning piece 332 are welded to the connecting plate 31. A pipe cover cylinder 333 is provided in the middle of the pipe cover 33. The pipe cover cylinder 333 can be movably inserted into the inside of the connecting piece 331 to close the opening slot 321.

[0040] Specifically, the opening of the end sleeve 111 is smaller than the opening of the pipe body 11, and the diameter of the connecting sleeve 12 is equal to the diameter of the pipe body 11.

[0041] In this embodiment, the end tube 111 is a part of the top of the tube body 11. The end tube 111 is used to connect with the connecting tube 12. The opening of the end tube 111 is smaller than the opening of the tube body 11. The diameter of the connecting tube 12 is equal to the diameter of the tube body 11, so that the end tube 111 is more tightly installed with the connecting tube 12, and the tube body 11 and the connecting tube 12 are continuous parts of the same cylinder.

[0042] Specifically, the dimensions of the tube cap 333 and the opening groove 321 are matched.

[0043] In this embodiment, the diameter of the tube cap 333 is matched with the width of the opening groove 321, so that the tube cap 333 can be smoothly inserted into the opening groove 321 without being subjected to excessive resistance or being damaged.

[0044] Specifically, the connecting piece 331 and the turning piece 332 are made of malleable material.

[0045] In this embodiment, the malleable material allows the connecting piece 331 and the bending piece 332 to adapt to different shapes and sizes during installation, thereby ensuring that the tube cap 33 can be firmly connected to the connecting plate 31.

[0046] The above are merely preferred embodiments of this application and are not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application. The above are merely preferred embodiments of this application. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of this application, and these improvements and modifications should also be considered within the protection scope of this application.

Claims

1. A double-layered cap eight-connector pipe, characterized in that: Includes an eight-pipe assembly (1), with an inner cover assembly (2) inserted into the top of the eight-pipe assembly (1), and an outer cover assembly (3) for covering the eight-pipe assembly (1) mounted on the upper surface of the inner cover assembly (2). The eight-pipe assembly (1) includes a pipe body (11) and a connecting cylinder (12). The connecting cylinder (12) is threaded onto the top of the pipe body (11), and a protrusion (122) is mounted around the end of the connecting cylinder (12). The inner cover assembly (2) includes a long plate (21), a limiting ring (22) and a connecting ring (23). The limiting ring (22) is installed at both ends of the upper surface of the limiting ring (22). The connecting ring (23) is evenly installed on the surface along the long plate (21). The surface of the connecting ring (23) is surrounded by a slot (231). A thin film (232) is installed inside the connecting ring (23). The protrusion (122) is inserted into the inside of the slot (231). The outer cover assembly (3) includes a connecting plate (31), a pipe cover groove (32), a pipe cover (33), a limiting post (34), and a sealing gasket (35). The limiting post (34) is fixedly installed at both ends of the lower surface of the connecting plate (31). The limiting post (34) is inserted into the limiting ring sleeve (22). Several sets of the pipe cover groove (32) are opened on the upper surface of the connecting plate (31). The pipe cover (33) is hinged to one side of the connecting plate (31). Several sets of the sealing gasket (35) are fixedly installed on the bottom surface of the connecting plate (31). The sealing gasket (35) is inserted into the inside of the connecting ring (23).

2. The double-layered cap eight-connector pipe according to claim 1, characterized in that: The top of the tube body (11) is provided with an end cylinder (111), the outside of the end cylinder (111) is provided with an external thread (112), the inside of the connecting cylinder (12) is provided with an internal thread (121), and the protrusion (122) and the end cylinder (111) are detachably installed.

3. The double-layered cap eight-connector pipe according to claim 1, characterized in that: The connecting plate (31) has an opening groove (321) in the middle, and a cover ring (322) is fixedly installed on the upper part of the opening groove (321). Angle grooves (323) are opened on both sides of the end of the pipe cover groove (32), and a hand-turning groove (324) is opened in the middle of the end of the pipe cover groove (32).

4. The double-layered cap eight-connector pipe according to claim 1, characterized in that: A connecting piece (331) and a turning piece (332) are provided on one side of the pipe cover (33). The connecting piece (331) and the turning piece (332) are fixedly connected to the connecting plate (31). A pipe cover cylinder (333) is provided in the middle of the pipe cover (33). The pipe cover cylinder (333) can be movably inserted into the inside of the connecting piece (331).

5. A double-layered cap eight-connector pipe according to claim 2, characterized in that: The opening of the end tube (111) is smaller than the opening of the tube body (11), and the diameter of the connecting tube (12) is equal to the diameter of the tube body (11).

6. The double-layered cap eight-connector pipe according to claim 4, characterized in that: The dimensions of the tube cap (333) are matched with the opening groove (321).

7. A double-layered cap eight-connector pipe according to claim 4, characterized in that: The connecting piece (331) and the turning piece (332) are made of malleable material.