Aerosol sampling device for pathogenic bacteria on surface of blade

Abstract

The utility model relates to the technical field of specimen sampling equipment, and provides a blade surface pathogenic bacteria aerosol sampling device which comprises an adapter, a sampling tube and a fairing, a first connecting part is arranged at one end of the adapter, a second connecting part is arranged at the other end of the adapter, and a through hole is formed between the first connecting part and the second connecting part; a guide tube is arranged in the second connecting part, a suction inlet is formed in one side of the second connecting part, and the suction inlet is communicated with the drainage area; the fairing is connected with the suction inlet, the suction inlet is communicated with the rectification space, the sampling pipe is connected with the second connecting part, and part of the pipe body of the guide pipe is located in the sampling pipe. The blade is placed in the fairing, negative pressure is input through the negative pressure port, pathogenic bacteria on the blade can be sampled by liquid in the sampling pipe under the action of the negative pressure, the whole process is achieved by starting a negative pressure device, the labor intensity is low, the sampling efficiency is high, sampling is achieved in a non-contact mode, and the sampling efficiency is high. And the risk of cross contamination in the collection process is reduced.

Description

Technical Field

[0001] This utility model relates to the technical field of specimen sampling equipment, and in particular to a leaf surface pathogen aerosol sampling device. Background Technology

[0002] Accurate detection of plant diseases requires the use of nucleic acid testing, which can be performed by swabbing or collecting leaves. The swab method is more expensive, and to avoid cross-contamination from contact testing, a new swab is needed for each leaf, resulting in a large workload for sample testing. Collecting leaves requires extracting pathogens from a large amount of leaf tissue, and the enrichment and extraction process is relatively complex. Utility Model Content

[0003] This invention provides a leaf surface pathogen aerosol sampling device to solve the shortcomings of existing technologies that use test paper for sampling, which are difficult and have a high risk of cross-contamination.

[0004] This invention provides a leaf surface pathogen aerosol sampling device, including an adapter, a sampling tube, and a rectifier. One end of the adapter has a first connecting portion for connection to a negative pressure port, and the other end has a second connecting portion with a hollow tubular structure. A through hole connects the first and second connecting portions. A guide tube is provided inside the second connecting portion, and a drainage area for gas flow is formed between the tube body of the guide tube and the inner wall of the second connecting portion. An intake port is located on one side of the second connecting portion in the drainage area and communicates with the drainage area. A rectification space for leaf insertion is formed inside the rectifier. The rectifier is connected to the intake port, and the intake port communicates with the rectification space. The sampling tube is connected to the second connecting portion, and part of the guide tube is located inside the sampling tube. Under negative pressure, the guide tube guides the airflow to disturb the liquid rotation inside the sampling tube, thereby achieving pathogen sampling.

[0005] According to the leaf surface pathogen aerosol sampling device provided by this utility model, the second connecting part is provided with an internal thread, and the opening of the sampling tube is threadedly connected to the second connecting part through the internal thread.

[0006] According to the leaf surface pathogen aerosol sampling device provided by this utility model, a communicating chamber is formed in the first connecting part, the bottom of the communicating chamber is provided with the through hole, the inner diameter of the communicating chamber is larger than the inner diameter of the through hole, the top of the communicating chamber is an open structure and is located in the negative pressure port.

[0007] According to the leaf surface pathogen aerosol sampling device provided by this utility model, a filter is provided in the communicating chamber.

[0008] According to the leaf surface pathogen aerosol sampling device provided by this utility model, the filter includes a high-efficiency particulate air filter.

[0009] According to the leaf surface pathogen aerosol sampling device provided by this utility model, the first connecting part includes a plurality of snap-fit ​​grooves for cooperating with the negative pressure port, and the plurality of snap-fit ​​grooves are evenly spaced on the outer peripheral wall of the adapter.

[0010] According to the leaf surface pathogen aerosol sampling device provided by this utility model, the rectifier includes a receiving part and a communicating part, the communicating part is connected to the receiving part, the rectifier space is formed in the receiving part, and the communicating part is connected to the inlet.

[0011] According to the leaf surface pathogen aerosol sampling device provided by this utility model, the receiving part includes a first cover and a second cover arranged vertically, with a gap between the first cover and the second cover, and the gap communicating with the rectification space.

[0012] According to the leaf surface pathogen aerosol sampling device provided by this utility model, both the first cover and the second cover are constructed as rectangular shell structures, and one side of the first cover and the second cover is provided with an opening structure.

[0013] According to the leaf surface pathogen aerosol sampling device provided by this utility model, a quick connector is provided at the inlet, and the corresponding connecting part is connected to the quick connector.

[0014] This utility model provides a leaf surface pathogen aerosol sampling device. By placing the leaf in a rectifier and inputting negative pressure through a negative pressure port, the pathogens on the leaf can be sampled by the liquid in the sampling tube under negative pressure. The entire process is achieved by turning on the negative pressure device, which has low labor intensity, high sampling efficiency, and reduces the risk of cross-contamination during the sampling process by achieving sampling in a non-contact manner. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0016] Figure 1 This is an exploded structural diagram of the leaf surface pathogen aerosol sampling device provided by this utility model.

[0017] Figure 2This is a schematic diagram of the internal connection structure of the second connecting part in the leaf surface pathogen aerosol sampling device provided by this utility model.

[0018] Figure label:

[0019] 1. Adapter; 11. First connecting part; 111. Snap-fit ​​groove; 12. Second connecting part; 121. Suction port; 122. Guide tube; 2. Negative pressure port; 3. Sampling tube; 4. Radiator; 41. Receiving part; 411. First cover; 412. Second cover; 413. Gap; 414. Opening structure; 42. Connecting part. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0021] In the description of the embodiments of this utility model, it should be noted that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the purpose of clarifying the embodiments of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this utility model. In addition, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0022] In the description of the embodiments of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this utility model according to the specific circumstances.

[0023] In this embodiment of the utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0024] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0025] When sampling pathogens on plant leaves, conventional techniques use test strips to sample one by one. This method is labor-intensive and carries a high risk of cross-contamination due to its contact sampling method.

[0026] Regarding the problems in related technologies, such as Figure 1 , Figure 2As shown, this utility model provides a leaf surface pathogen aerosol sampling device, including an adapter 1, a sampling tube 3, and a shroud 4; one end of the adapter 1 is provided with a first connecting part 11 for connecting to a negative pressure port 2, and the other end of the adapter 1 is provided with a second connecting part 12 with a hollow tubular structure, and a through hole is provided between the first connecting part 11 and the second connecting part 12 to connect the first connecting part 11 and the second connecting part 12; a guide tube 122 is provided inside the second connecting part 12, and the tube body of the guide tube 122 and the inner wall of the second connecting part 12 form a connection. A flow-guiding area for gas flow is provided, and an intake port 121 is provided on one side of the second connecting part 12 in the flow-guiding area, communicating with the flow-guiding area. A rectifier space for blade insertion is formed inside the rectifier shroud 4, which is connected to the intake port 121, and the intake port 121 is connected to the rectifier space. The sampling tube 3 is connected to the second connecting part 12, and part of the guide tube 122 is located inside the sampling tube 3. Under negative pressure, the guide tube 122 guides the airflow to disturb the liquid rotation in the sampling tube 3, thereby achieving pathogen sampling. Pathogens are usually located on the surface of the blade. During sampling, it is necessary to collect the pathogens on the surface. In this example, the negative pressure port 2 can provide negative pressure, thereby generating negative pressure airflow. The negative pressure airflow can flow over the blade surface at high speed, so that the blade surface is subjected to airflow shear force, achieving the release of pathogens. The released pathogens follow the airflow into the sampling tube 3, and the liquid in the sampling tube 3 rotates under the action of the airflow, thus achieving pathogen sampling. The entire process requires no human contact, which improves sampling efficiency and reduces the risk of cross-contamination during sampling.

[0027] Specifically, sampling tube 3 is filled with sampling solution, which is a sterilized 0.5% Tween-20 solution. Negative pressure port 2 is connected to a negative pressure generating device (such as a vacuum pump, negative pressure fan, etc.). The negative pressure generating device generates negative pressure and creates a negative pressure flow path between adapter 1 and rectifier 4, thereby realizing the collection of pathogens on the leaf surface.

[0028] like Figure 2 As shown, the guide tube 122 has a tubular structure, with the opening at the bottom of the guide tube 122 located inside the sampling tube 3. The guide tube 122 and the second connecting part 12 are concentrically arranged. The airflow enters through the air intake 121 in the drainage area and then into the sampling tube 3. The airflow causes the sampling liquid inside the sampling tube 3 to rotate (e.g., ...). Figure 2 The dashed line indicates the airflow direction, causing pathogens to collide with the sampling liquid due to inertia during the rotational motion, thus achieving sampling of ice-borne bacteria. The entire process requires no manual operation, reducing the difficulty of operation.

[0029] In conjunction with the above embodiments, the second connecting part 12 is provided with an internal thread, and the opening of the sampling tube 3 is threadedly connected to the second connecting part 12 through the internal thread. During continuous sampling, multiple sampling tubes 3 may be required. In this embodiment, the sampling tubes 3 are connected by threads, which allows for quick connection and disassembly, facilitating continuous sampling operations.

[0030] Specifically, the sampling tube 3 has an external thread section at the tube opening. The external thread section and the internal thread are matched to achieve a threaded connection. The threaded connection can make the connection between the sampling tube 3 and the second connecting part 12 a sealed connection, and can also enable the sampling tube 3 to be installed and disassembled quickly.

[0031] In a specific configuration, a sealing ring is provided at the top of the internal thread section of the second connecting part 12. The sealing performance between the sampling tube 3 and the second connecting part 12 is improved by the setting of the sealing ring.

[0032] According to some embodiments of this utility model, a communicating chamber is formed within the first connecting portion 11. A through hole is provided at the bottom of the communicating chamber, and the inner diameter of the communicating chamber is larger than the inner diameter of the through hole. The top of the communicating chamber is an open structure 414 located within the negative pressure port 2. The negative pressure generated by the negative pressure generating device communicates with the second connecting portion 12 through the open structure 414 and is transmitted to the rectifier 4, thereby enabling the collection of pathogens from the leaf surface. In this embodiment, the communicating chamber facilitates airflow and optimizes the distribution of negative pressure.

[0033] Specifically, the inner diameter of the connecting chamber is much larger than the inner diameter of the through hole. The larger connecting chamber reduces the airflow velocity within it, while the smaller diameter of the second connecting part 12 increases the airflow velocity. This facilitates the rotation of the sampling liquid within the sampling tube 3, increasing its rotation speed and simplifying sampling. In other words, this method allows for a stronger negative pressure at the suction inlet 121, enabling effective collection of pathogens from the leaf surface.

[0034] In conjunction with the above embodiments, a filter is provided in the connecting chamber. The filter prevents adsorbed impurities from entering the negative pressure generating device, thus avoiding secondary contamination.

[0035] In specific setups, the filter includes a high-efficiency particulate air (HEPA) filter. By using an HEPA filter, it is possible to effectively filter out tiny particles such as dust, pollen, smoke, bacteria, viruses, and mold spores, thus preventing secondary pollution.

[0036] Specifically, the high-efficiency particulate air filter is shaped like a frustum, with its smaller diameter end located at the bottom of the connecting chamber, and this smaller diameter end being the bottom surface with a filter screen. The larger diameter end faces the negative pressure port 2 and is an open structure. This design effectively filters fine particles and reduces the impact on the negative pressure intensity.

[0037] In some embodiments, the first connecting portion 11 includes a plurality of snap-fit ​​grooves 111 for engaging with the negative pressure port 2, and the plurality of snap-fit ​​grooves 111 are evenly spaced on the outer peripheral wall of the adapter 1. The snap-fit ​​grooves 111 enable snap-fit ​​installation, which facilitates the loading and unloading of the adapter 1.

[0038] Specifically, multiple protruding structures are provided on the inner wall surface of the negative pressure port 2, and the protruding structures are set one-to-one with the snap-fit ​​grooves 111, so that the two can be snapped together through the snap-fit ​​grooves 111, thereby improving the convenience of connection.

[0039] In some embodiments, such as Figure 1 As shown, the fairing 4 includes a receiving portion 41 and a connecting portion 42. The connecting portion 42 is connected to the receiving portion 41, and a rectification space is formed inside the receiving portion 41. The connecting portion 42 is connected to the intake port 121. This arrangement facilitates the fabrication and installation of the fairing 4.

[0040] Specifically, the rectifier space is used to insert the blades, while the connecting part 42 can be connected to the inlet 121. During the preparation of the rectifier 4, the two parts can be produced separately, which is conducive to large-scale production.

[0041] In a specific configuration, a quick-connect fitting is provided at the intake 121, and the corresponding connecting part 42 is connected to the quick-connect fitting. The quick-connect fitting enables a quick connection between the two, thereby facilitating the replacement of the fairing 4.

[0042] Specifically, different specifications of fairing 4 may be used for different blades. In this embodiment, quick-connect fittings are provided to enable quick assembly and disassembly.

[0043] The quick connector can be a quick-connect connector in conventional technology. By setting a quick-connect connector at the suction port 121, the two can be quickly connected.

[0044] In conjunction with the above embodiments, the receiving part 41 includes a first cover 411 and a second cover 412 arranged vertically, with a gap 413 between the first cover 411 and the second cover, the gap 413 communicating with the rectification space. The gap 413 facilitates the insertion of the blades and reduces the difficulty of operation.

[0045] Specifically, the upper and lower covers can form a rectification space. During sampling, the blade is located in the rectification space, and negative pressure airflow is applied to collect pathogens on the blade surface.

[0046] During operation, the blade can be inserted into the rectification space through the gap 413, thereby enabling the collection of pathogens on the blade surface.

[0047] In a specific configuration, both the first cover 411 and the second cover 412 are constructed as rectangular shell structures, and an opening structure 414 is provided on one side of both the first cover 411 and the second cover 412. The opening structure 414 further reduces the difficulty of operation and enables rapid insertion of the blade.

[0048] like Figure 1 As shown, both sides of the first cover 411 and the second cover 412 are provided with slits 413, and the opening structure 414 enlarges the slits 413 on the front side of the first cover 411 and the second cover 412, so that the blades can be easily placed into the rectification space. Larger blades extend through the slits 413 on both sides, avoiding the curling of the blades and facilitating the rapid insertion of the blades, so as to realize the collection of pathogens on the surface of the blades.

[0049] The specific sampling process is as follows: At the start of sampling, add 2 mL of sterilized 0.5% Tween-20 solution to sampling tube 3 (a disposable 5 mL centrifuge tube). Then, install sampling tube 3 onto adapter 1 via the threaded connection. Next, insert the leaf into the rectifier 4 and turn on the negative pressure generator (such as a vacuum cleaner) at maximum power for 1 minute to maximize the shearing effect of the airflow. Sampling can be stopped after collecting one leaf or multiple leaves can be collected continuously. When sampling multiple leaves continuously, this device can enrich pathogens from multiple leaf surfaces into one sampling tube 3, and due to the HEPA filter, there is no risk of cross-contamination. After sampling is complete, remove sampling tube 3, seal it, and number it. Subsequently, bacterial DNA is extracted from the sample solution using a bacterial DNA extraction kit, then amplified using PCR technology, and detected using DNA gel electrophoresis.

[0050] The following is an illustration through specific experimental examples.

[0051] Taking cucumber bacterial angular leaf spot as an example, a large number of pathogens are present in the apoplasts of leaf tissues during the early stages of infection, and are released through stomata via plant transpiration. This device was used to sample leaves in greenhouses at risk of cucumber bacterial angular leaf spot. Twenty leaves were randomly selected from one greenhouse for sampling. Bacterial DNA was extracted from the sample solution using a bacterial DNA extraction kit, amplified by PCR, and detected by DNA gel electrophoresis. In another greenhouse without risk of cucumber bacterial angular leaf spot, 20 leaves were randomly selected and sampled using the same analytical method. The results showed that samples from the greenhouse at risk of disease were positive, while those from the greenhouse without risk were negative. A disease index survey was conducted 7 days later. The disease index in the positive greenhouse reached 29.75, while it was 0 in the negative greenhouse, indicating no disease. This demonstrates that this device can achieve rapid pathogen sampling without cross-contamination during the sampling process.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A device for sampling airborne pathogens on leaf surfaces, characterized in that, The utility model relates to a sampling device for pathogenic bacteria, comprising: an adapter, a sampling tube and a fairing; one end of the adapter is provided with a first connecting part for connecting with a negative pressure port, the other end of the adapter is provided with a second connecting part with a hollow tubular structure, a through hole is arranged between the first connecting part and the second connecting part to communicate the first connecting part and the second connecting part; a guide tube is arranged in the second connecting part, a drainage area for gas flow is formed between the tube body of the guide tube and the inner wall of the second connecting part, a suction port is arranged on one side of the second connecting part in the drainage area, and the suction port communicates with the drainage area; a fairing space for blade extension is formed in the fairing, the fairing is connected with the suction port, the suction port communicates with the fairing space, the sampling tube is connected with the second connecting part, and part of the tube body of the guide tube is located in the sampling tube to guide the rotation of liquid in the sampling tube under the action of negative pressure, so that pathogenic bacteria are sampled.

2. The leaf surface pathogen aerosol sampling device of claim 1, wherein, An internal thread is arranged in the second connecting part, and the tube opening of the sampling tube is threadedly connected with the second connecting part through the internal thread.

3. The leaf surface pathogen aerosol sampling device of claim 1, wherein, A communication chamber is formed in the first connecting part, the through hole is arranged at the bottom of the communication chamber, the inner diameter of the communication chamber is larger than that of the through hole, the top of the communication chamber is an open structure and is located in the negative pressure port.

4. The leaf surface pathogen aerosol sampling device of claim 3, wherein, A filter is arranged in the communication chamber.

5. The leaf surface pathogen aerosol sampling device of claim 4, wherein, The filter comprises a high-efficiency particulate air filter.

6. The leaf surface pathogen aerosol sampling device of claim 1, wherein, The first connecting part comprises a plurality of clamping grooves for cooperating with the negative pressure port, and the plurality of clamping grooves are uniformly and spacedly arranged on the outer peripheral wall of the adapter.

7. The leaf surface pathogen aerosol sampling device of claim 1, wherein, The fairing comprises a containing part and a communication part, the communication part is connected with the containing part, the fairing space is formed in the containing part, and the communication part is connected with the suction port.

8. The leaf surface pathogen aerosol sampling device of claim 7, wherein, The containing part comprises a first cover body and a second cover body arranged in an up-down manner, a gap is arranged between the first cover body and the second cover body, and the gap communicates with the fairing space.

9. The leaf surface pathogen aerosol sampling device of claim 8, wherein, The first cover body and the second cover body are both configured as rectangular shell structures, and one side of the first cover body and the second cover body is provided with an open structure.

10. The leaf surface pathogen aerosol sampling device of claim 7, wherein, A quick connector is arranged at the suction port, and the communication part is connected with the quick connector.

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