Multi-connected sampling device of bioreactor
The design of a multi-sampling device for bioreactors solves the problems of cross-contamination and energy waste caused by single sampling, and enables multiple aseptic sampling and flexible sample collection, ensuring the safe flow of samples and aseptic operation within the bioreactor.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-27
- Publication Date
- 2026-03-03
AI Technical Summary
The existing single sampling method in bioreactors is prone to cross-contamination and energy waste, and cannot achieve multiple sterile sampling.
Design a multi-sampling device for a bioreactor, using components such as a three-way sampling valve, a Luer three-way valve, a Robert clamp, and a disc filter to achieve series connection of multiple sampling containers, ensuring the safety and flexibility of the aseptic sampling process.
It enables multiple aseptic samplings, avoids cross-contamination, saves energy, adapts to the sampling needs of different process stages, and has a simple structure and is easy to operate.
Smart Images
Figure CN223963515U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of bioreactor culture and detection technology, and in particular relates to a multi-sampling device for bioreactors. Background Technology
[0002] Bioreactor culture is the mainstream method for production in the biopharmaceutical industry. During cell expansion culture, it is essential to observe cell growth over a specific period to ensure normal cell proliferation and growth, free from abnormalities or contamination. This includes monitoring and recording parameters such as culture medium pH, cell count, and viability. Real-time observation necessitates sampling cells from the bioreactor at prescribed intervals. Since the bioreactor is a sterile environment, strict aseptic techniques are required during sampling. Improper sampling, such as using a non-closed system or frequent container changes, can lead to contamination of the culture medium within the bioreactor, resulting in significant economic losses for the production company.
[0003] Currently, most bioreactor sampling is a single, one-time sampling. If cell culture in the bioreactor needs to be carried out for a long time, multiple samplings are required to observe the cell growth status. This requires changing the sampling bottle multiple times and sterilizing the sampling valve again before the next sampling can be carried out. Each sterilization process of the sampling valve requires a pure steam supply, resulting in a waste of energy and money. Utility Model Content
[0004] The purpose of this invention is to provide a multi-sampling device for bioreactors to address the shortcomings of existing technologies.
[0005] To achieve the above-mentioned objectives, the technical solution adopted by this utility model is as follows:
[0006] A multi-port sampling device for a bioreactor includes: a three-way sampling valve, the three ports of which are respectively connected to a bioreactor, a solenoid valve, and a stainless steel straight pipe via pipelines; the other end of the stainless steel straight pipe is connected to a first thermoplastic tube; the other end of the first thermoplastic tube is connected to a multi-port sampling container via a male Luer connector; the other end of the multi-port sampling container is connected to a waste liquid bag via a female Luer connector and pipelines; and a disc filter is connected to the waste liquid bag via a pipeline.
[0007] Furthermore, the multi-sampling container includes: multiple Luer three-way valves connected in series, each Luer three-way valve's output end being connected to a sampling bottle via a pipeline, and the cap of the sampling bottle being connected to a disc filter via another pipeline.
[0008] Furthermore, Robert clamps are installed on the pipeline between the first thermoplastic tube, the Luer three-way valve and the sampling bottle, and the pipeline between the Luer three-way valve and the waste liquid bag.
[0009] Furthermore, a stainless steel tee is installed on the pipeline between the three-way sampling valve and the stainless steel straight pipe, and the output end of the stainless steel tee is connected to a second thermoplastic tube, on which a Robert clamp is installed.
[0010] The multi-sampling device for bioreactors provided by this utility model has the following advantages compared with the prior art:
[0011] 1. This utility model perfectly combines a multi-sampling container and a three-way sampling valve through a first thermoplastic tube. The multi-sampling container effectively avoids cross-contamination during the sampling process, and can perform sterile and safe sampling at any stage of the process, achieving reliable process testing. It is also simple in structure, convenient and quick to operate.
[0012] 2. This utility model uses a combination of Luer three-way valve and Robert clamp design, which allows for convenient and safe use. At the same time, the use of disc filter and pipeline connection ensures that the bioreactor and sampling bottle maintain the same atmospheric pressure, ensuring that the sample in the bioreactor flows smoothly into the sampling bottle (if a traditional sealed sampling bag is used, it is difficult for the sample to flow out of the bioreactor into the sampling bag), and avoids the entry of contaminants from the port, ensuring zero risk of cross-contamination.
[0013] 3. The sampling quantity of this utility model can be increased or decreased according to the actual number of culture days and sampling requirements. The size of the sampling bottle can also be designed in different sizes according to the actual sample demand. It is very flexible and can be fully applied to various types of sampling requirements, making it widely applicable. Attached Figure Description
[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.
[0015] Figure 1 This is a schematic diagram of the structure of this utility model;
[0016] Figure 2 This is a schematic diagram of the structure of the multi-unit sampling container of this utility model;
[0017] Figure 3 This is a diagram showing the positional relationship between the first thermoplastic tube and the second thermoplastic tube of this utility model.
[0018] In the diagram: 1-Three-way sampling valve, 2-Solenoid valve, 3-Stainless steel straight pipe, 4-First thermoplastic tube, 5-Waste liquid bag, 6-Luer three-way valve, 7-Sampling bottle, 8-Disc filter, 9-Robert clamp, 10-Stainless steel tee, 11-Second thermoplastic tube, 12-Male Luer connector, 13-Female Luer connector. Detailed Implementation
[0019] The technical solution of this utility model will be further described below with reference to the accompanying drawings and embodiments:
[0020] refer to Figure 1-3 As shown, this utility model provides a multi-port sampling device for a bioreactor, comprising: a three-way sampling valve 1, the three ports of which are respectively connected to a bioreactor, a solenoid valve 2, and a stainless steel straight pipe 3 via pipelines; the other end of the stainless steel straight pipe 3 is connected to a first thermoplastic tube 4; the other end of the first thermoplastic tube 4 is connected to a multi-port sampling container via a male Luer connector 12; the other end of the multi-port sampling container is connected to a waste liquid bag 5 via a female Luer connector 13 and pipelines; and a disc filter 8 is connected to the waste liquid bag 5 via a pipeline. By combining the multi-port sampling container, the three-way sampling valve 1, and the bioreactor, the potential risks of cross-contamination during sampling are effectively avoided, making it suitable for aseptic sampling at different process stages.
[0021] In a preferred embodiment, the multi-port sampling container includes multiple Luer three-way valves 6 connected in series. The output end of each Luer three-way valve 6 is connected to a sampling bottle 7 via a pipe. The cap of each sampling bottle 7 is connected to a disc filter 8 via another pipe. The number of Luer three-way valves 6 and sampling bottles 7 can be increased or decreased according to the actual number of culture days and sampling requirements. The size of the sampling bottles 7 can also be designed in different sizes according to the actual sample volume required, making it highly flexible and fully applicable to various types of sampling requirements, with wide applications.
[0022] In a preferred embodiment, Robert clamps 9 are provided on the pipeline between the first thermoplastic tube 4, the Luer three-way valve 6 and the sampling bottle 7, and on the pipeline between the Luer three-way valve 6 and the waste liquid bag 5. The combination of the Luer three-way valve 6 and the Robert clamp 9 allows for convenient and safe retrieval, while the use of a disc filter 8 prevents contaminants from entering through the port, ensuring zero risk of cross-contamination.
[0023] In a preferred embodiment, a stainless steel tee 10 is installed on the pipeline between the three-way sampling valve 1 and the stainless steel straight pipe 3. The output end of the stainless steel tee 10 is connected to a second thermoplastic tube 11, and a Robert clamp 9 is installed on the second thermoplastic tube 11. Through the stainless steel tee 10 and the second thermoplastic tube 11 at its output end, multiple sampling containers can be added according to sampling requirements, thereby improving the adaptability of the multi-sampling device.
[0024] In a preferred embodiment, all of the above-mentioned pipelines are made of silicone tubing.
[0025] The working process and principle of this utility model are as follows:
[0026] a. Connect the disposable multi-unit sampling container to the stainless steel straight tube 3 via the first thermoplastic tube 4 using the BWTC aseptic connector;
[0027] b. Ensure that the eight Luer three-way valves 6 on the disposable multi-port sampling container are in the closed state of the sampling bottle 7 and are interconnected and connected to the 1L waste liquid bag 5;
[0028] c. First open the Robert clamp 9 on the first thermoplastic tube 4 and the silicone tube connected to the 1L waste liquid bag 5, then open the three-way sampling valve 1. The waste liquid is then forced into the first thermoplastic tube 4 by the pressure in the bioreactor, and then reaches the Luer three-way valve 6 through the first thermoplastic tube 4, and directly to the 1L waste liquid bag 5 until the waste liquid is discharged.
[0029] d. Clamp the Robert clamp 9 on the silicone tube connecting the waste liquid bag 5;
[0030] e. Rotate the first Luer three-way valve 6 connected to the first thermoplastic tube 4 to connect the first sampling bottle 7 to the first thermoplastic tube 4;
[0031] f. Open the Robert clip 9 on the silicone tube connected to the first sampling bottle 7 and take a sample (random sample amount as needed);
[0032] g. Clamp the Robert clamp 9 on the silicone tube connected to the first sampling bottle 7;
[0033] h. Rotate the first Luer three-way valve 6 back to the state of step b, close the three-way sampling valve 1 and clamp the Robert clamp 9 on the first thermoplastic tube 4;
[0034] i. Disconnect the sampling bottle 7 from the disposable multi-unit sampling container to complete the sampling operation;
[0035] j. When taking a new sample, repeat step bi.
[0036] In the description of this application, it should be noted that the terms "upper," "lower," etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. Unless otherwise expressly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication between two elements. For those skilled in the art, the specific meaning of the above terms in this application can be understood according to the specific circumstances.
[0037] It should be noted that in this application, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.
[0038] The above description is merely a specific embodiment of this application, enabling those skilled in the art to understand or implement this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features claimed herein.
Claims
1. A multi-port sampling device for a bioreactor, comprising: The utility model relates to a kind of biological reaction system, including: Three-way sampling valve (1), three interfaces of the three-way sampling valve (1) are connected with biological reactor, solenoid valve (2) and stainless steel straight pipe (3) respectively by pipeline, the other end of the stainless steel straight pipe (3) is connected with first thermoplastic pipe (4), the other end of the first thermoplastic pipe (4) is connected with multiple sampling container by male luer joint (12), the other end of the multiple sampling container is connected with waste liquid bag (5) by female luer joint (13) and pipeline, disc filter (8) is connected on the waste liquid bag (5) by pipeline.
2. The multiport sampling device for a bioreactor of claim 1, wherein, The multiple sampling container includes: a plurality of luer three-way valves (6) are connected in series, the output end of each luer three-way valve (6) is connected with sampling bottle (7) by pipeline, the cover of the sampling bottle (7) is connected with disc filter (8) by another pipeline.
3. The multiport sampling device for a bioreactor of claim 2, wherein, Robert clamp (9) is arranged on the pipeline between the first thermoplastic pipe (4), luer three-way valve (6) and sampling bottle (7) and the pipeline between luer three-way valve (6) and waste liquid bag (5).
4. The multiport sampling device for a bioreactor of claim 2, wherein, Stainless steel tee (10) is arranged on the pipeline between three-way sampling valve (1) and stainless steel straight pipe (3), the output end of the stainless steel tee (10) is connected with second thermoplastic pipe (11), and Robert clamp (9) is arranged on the second thermoplastic pipe (11).