Automatic pretreatment equipment for protein desalination

Abstract

The utility model discloses automatic pretreatment equipment for protein desalination, and relates to the technical field of protein desalination, the automatic pretreatment equipment comprises a reagent bearing frame and a proportioning assembly, the inner side of the reagent bearing frame is connected with a reagent bottle in a clamping manner, the right side of the reagent bearing frame is provided with a protective cavity, the proportioning assembly is arranged in the protective cavity, and the reagent bottle is connected with the proportioning assembly. The proportioning assembly comprises a supporting seat, a quaternary gradient proportional valve, a pure organic pipeline, a pure water phase pipeline, an eluent pipeline, a proportioning and mixing pipeline, a flowing pipe, a pumping pump, a connecting pipe and a mixer, and the supporting seat is arranged in the protective cavity. According to the automatic pretreatment equipment for protein desalination, organic solvents and water phases with different concentrations can be automatically or manually mixed according to requirements through the proportioning assembly, the reagents and the mixing proportion can be adjusted according to actual application scenes, accurate mixing of liquid phases in the elution process is guaranteed, and the accuracy of the pretreatment equipment is improved. Through the auxiliary assembly, the device can accurately control the needle head to move, and the desalting efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of protein desalting technology, specifically to an automated pretreatment device for protein desalting. Background Technology

[0002] In proteomics research, sample pretreatment is a crucial step in ensuring the accuracy and reliability of experimental results. The extraction, enzymatic digestion, separation, and analysis of proteins in cells or tissues often involve complex separation and purification processes, among which desalting is a vital step. Desalting typically removes salts, solvents, and small molecule impurities from the sample to ensure the accuracy and efficiency of subsequent analytical steps. Therefore, an automated protein desalting pretreatment device is needed. However, current automated protein desalting pretreatment devices still have the following shortcomings:

[0003] For example, patent document CN222174142U discloses a protein desalting device. This protein desalting device eliminates the need to hold the first tube sleeve during the entire process, improving the convenience of the experiment and solving the problem that the first tube sleeve lacks related fixing equipment, thus requiring manual operation and reducing the convenience of the experiment. However, it cannot accurately control the required reagent ratio and is not convenient to adjust the ratio of organic solvent to water phase as needed, resulting in poor desalting efficiency in actual use.

[0004] Therefore, in view of this, we have studied and improved the existing structure to address its shortcomings and proposed an automated protein desalting pretreatment device. Utility Model Content

[0005] The purpose of this invention is to provide an automated pretreatment device for protein desalting to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an automated pretreatment device for protein desalting, comprising a reagent carrier frame and a proportioning component. A reagent bottle is snapped into the inner side of the reagent carrier frame, and a protective cavity is provided on the right side of the reagent carrier frame. The proportioning component is located inside the protective cavity and includes a support base, a quaternary gradient proportioning valve, a pure organic pipeline, a pure aqueous phase pipeline, an elution buffer pipeline, a proportioning and mixing pipeline, a flow pipe, a pump, a connecting pipe, and a mixer. The support base is located inside the protective cavity, and a quaternary gradient proportioning valve is installed on the upper side of the support base. The left end of the quaternary gradient proportioning valve is connected to the pure organic pipeline, and the rear end of the quaternary gradient proportioning valve is provided with a pure aqueous phase pipeline.

[0007] Furthermore, the front end of the quaternary gradient proportional valve is connected to an eluent pipeline, and a proportioning and mixing pipeline is provided on the upper side of the quaternary gradient proportional valve.

[0008] Furthermore, the outer end of the mixing pipeline is connected to a flow pipe, and a pump is installed at the right end of the flow pipe.

[0009] Furthermore, the pump is connected to a connecting pipe on its right end, and a mixer is provided at the rear end of the connecting pipe.

[0010] Furthermore, a connecting base plate is installed at the bottom of the protective cavity, and a desalination treatment machine body is installed on the rear side of the upper surface of the connecting base plate. A conveying pipeline is connected to the rear side of the desalination treatment machine body, and the front end of the conveying pipeline is connected to the rear end of the mixer.

[0011] Furthermore, the upper hinge of the desalination machine body is connected to a protective cover, and telescopic support rods are rotatably connected to both sides inside the protective cover. The lower ends of the telescopic support rods are rotatably connected to both sides inside the desalination machine body.

[0012] Furthermore, the desalination machine body is equipped with auxiliary components for assistance, including a servo motor, a threaded screw, a connector, a support plate, a hydraulic push rod, and a conveying needle. The servo motor is located on the right side inside the desalination machine body, and the output end of the servo motor is connected to the threaded screw through a coupling.

[0013] Furthermore, the outer surface of the threaded screw is threaded with a connector, and a support plate is installed on the front side of the connector. A hydraulic push rod is provided on the upper surface of the support plate, and a conveying needle is installed at the lower end of the hydraulic push rod. A sample placement plate is installed on the inner surface of the desalination machine body.

[0014] This invention provides an automated pretreatment device for protein desalting, which has the following advantages:

[0015] 1. This utility model incorporates a mixing assembly, which includes a support base, a quaternary gradient proportioning valve, a pure organic pipeline, a pure aqueous phase pipeline, an eluent pipeline, a mixing pipeline, a flow tube, a pump, a connecting pipe, and a mixer. In use, a high-concentration acetonitrile solution is provided through the pure organic pipeline for separating and removing small molecule impurities incompatible with the target molecules. The pure aqueous phase pipeline provides an aqueous phase containing 0.1% formic acid for sample washing and dilution. A 60% acetonitrile and 0.1% formic acid aqueous solution is mixed through the eluent pipeline for desalting and further separation. The quaternary gradient proportioning valve adjusts the reagent ratios provided by the three pipelines, and the reagents are delivered to the flow tube through the mixing pipeline. The pump is activated to deliver the reagents through the connecting pipe to the mixer for thorough mixing. This allows the device to automatically or manually mix different concentrations of organic solvents and aqueous phases as needed, and to adjust the reagents and mixing ratios according to the actual application scenario, ensuring precise mixing of the liquid phase during elution.

[0016] 2. This utility model incorporates auxiliary components, including a servo motor, a threaded screw, a connector, a support plate, a hydraulic push rod, and a delivery needle. In use, the servo motor is activated, causing the threaded screw to rotate. This, in turn, moves the connector along the surface of the threaded screw, moving the delivery needle above the sample. The hydraulic push rod is then activated, causing the delivery needle to move downwards. This allows the delivery needle body to draw the reagent from the corresponding reagent bottle into the sample well for desalination. This allows the device to precisely control the needle movement, improving desalination efficiency. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural diagram of an automated protein desalting pretreatment device according to the present invention;

[0018] Figure 2 This is a rear-view three-dimensional structural diagram of an automated protein desalting pretreatment device according to the present invention;

[0019] Figure 3 This is a three-dimensional structural diagram of the proportioning component of an automated protein desalting pretreatment device according to the present invention;

[0020] Figure 4 This is a schematic diagram of the internal three-dimensional structure of the desalting machine body of an automated protein desalting pretreatment device according to the present invention;

[0021] Figure 5 This is a three-dimensional structural diagram of the auxiliary components of an automated protein desalting pretreatment device according to the present invention.

[0022] In the diagram: 1. Reagent carrier frame; 2. Reagent bottle; 3. Protective cavity; 4. Proportioning component; 401. Support base; 402. Quaternary gradient proportional valve; 403. Pure organic phase pipeline; 404. Pure aqueous phase pipeline; 405. Elution buffer pipeline; 406. Proportioning and mixing pipeline; 407. Flow tube; 408. Pump; 409. Connecting pipe; 410. Mixer; 5. Connecting base plate; 6. Desalination treatment body; 7. Delivery pipeline; 8. Protective cover; 9. Telescopic support rod; 10. Auxiliary component; 1001. Servo motor; 1002. Threaded screw; 1003. Connector; 1004. Support plate; 1005. Hydraulic push rod; 1006. Delivery needle; 11. Sample placement plate. Detailed Implementation

[0023] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0024] like Figures 1 to 5As shown, an automated protein desalting pretreatment device includes a reagent carrier frame 1 and a proportioning component 4. A reagent bottle 2 is snapped into the inner side of the reagent carrier frame 1, and a protective cavity 3 is located on the right side of the reagent carrier frame 1. The proportioning component 4 is disposed inside the protective cavity 3 and includes a support base 401, a quaternary gradient proportioning valve 402, a pure organic phase pipeline 403, a pure aqueous phase pipeline 404, an eluent pipeline 405, a proportioning and mixing pipeline 406, a flow tube 407, a pump 408, a connecting pipe 409, and a mixer 410. The support base 401 is disposed inside the protective cavity 3, and the support base 401 is snapped into the inner side of the reagent carrier frame 1. A quaternary gradient proportional valve 402 is installed on the upper side of the support 401. A pure organic pipeline 403 is connected to the left end of the quaternary gradient proportional valve 402, and a pure aqueous phase pipeline 404 is provided at the rear end of the quaternary gradient proportional valve 402. An eluent pipeline 405 is connected to the front end of the quaternary gradient proportional valve 402, and a proportioning and mixing pipeline 406 is provided on the upper side of the quaternary gradient proportional valve 402. A flow pipe 407 is connected to the outer end of the proportioning and mixing pipeline 406, and a pump 408 is installed at the right end of the flow pipe 407. A connecting pipe 409 is connected to the right end of the pump 408, and a mixer 410 is provided at the rear end of the connecting pipe 409.

[0025] The specific operation is as follows: During use, a high-concentration acetonitrile solution is provided through the pure organic pipeline 403 to separate and remove small molecule impurities that are incompatible with the target molecules. The pure aqueous phase pipeline 404 provides an aqueous phase containing 0.1% formic acid for washing and diluting the sample. A 60% acetonitrile and 0.1% formic acid aqueous solution is mixed through the eluent pipeline 405 for desalting and further separation. The reagent ratio provided by the three pipelines is adjusted through the quaternary gradient proportional valve 402 and delivered to the flow tube 407 through the proportioning and mixing pipeline 406. The pump 408 is started to deliver the reagent to the mixer 410 through the connecting pipe 409 for thorough mixing.

[0026] Please refer to Figure 2 , Figure 4 and Figure 5A connecting base plate 5 is installed at the bottom of the protective cavity 3, and a desalination treatment machine body 6 is installed on the rear side of the upper surface of the connecting base plate 5. A conveying pipe 7 is connected to the rear side of the desalination treatment machine body 6, and the front end of the conveying pipe 7 is connected to the rear end of the mixer 410. A protective cover 8 is hinged on the upper side of the desalination treatment machine body 6, and telescopic support rods 9 are rotatably connected to both sides inside the protective cover 8. The lower end of the telescopic support rods 9 is rotatably connected to both sides inside the desalination treatment machine body 6. An auxiliary component 10 for auxiliary purposes is set inside the desalination treatment machine body 6, and the auxiliary component 10 includes a servo motor 1001, a threaded screw 1002, and a connecting... The desalination machine body 6 includes a component 1003, a support plate 1004, a hydraulic push rod 1005, and a conveying needle 1006. A servo motor 1001 is located inside the right side of the desalination machine body 6. The output end of the servo motor 1001 is connected to a threaded screw 1002 via a coupling. A connector 1003 is threaded onto the outer surface of the threaded screw 1002. A support plate 1004 is installed on the front side of the connector 1003. A hydraulic push rod 1005 is installed on the upper surface of the support plate 1004. A conveying needle 1006 is installed at the lower end of the hydraulic push rod 1005. A sample placement plate 11 is installed on the inner surface of the desalination machine body 6.

[0027] The specific operation is as follows: When in use, start the servo motor 1001, which drives the threaded screw 1002 to rotate, thereby driving the connector 1003 to move along the surface of the threaded screw 1002, so that the delivery needle 1006 moves above the sample. Start the hydraulic push rod 1005, which drives the delivery needle 1006 to move downward, so that the body of the delivery needle 1006 draws the reagent in the corresponding reagent bottle 2 into the sample well for desalting treatment.

[0028] In summary, as Figures 1 to 5 As shown, this automated protein desalting pretreatment equipment, in use, firstly provides a high-concentration acetonitrile solution through the pure organic phase line 403 to separate and remove small molecule impurities incompatible with the target molecule. The pure aqueous phase line 404 provides an aqueous phase containing 0.1% formic acid for sample washing and dilution. The eluent line 405 mixes a 60% acetonitrile and 0.1% formic acid aqueous solution for desalting and further separation. The reagent ratios provided by the three lines are adjusted by the quaternary gradient proportional valve 402 and then delivered to the flow tube 407 through the mixing line 406. The pump 408 is started to deliver the reagent through the connecting tube 409 to the mixer 410 for thorough mixing. Then, the servo motor 1001 is started to drive the threaded screw 1002 to rotate, which in turn drives the connector 1003 to move along the surface of the threaded screw 1002, so that the delivery needle 1006 moves above the sample. The hydraulic push rod 1005 is started to drive the delivery needle 1006 to move downward, so that the body of the delivery needle 1006 draws the reagent in the corresponding reagent bottle 2 into the sample well for desalting.

[0029] The embodiments of this utility model are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the utility model to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical applications of this utility model, and to enable those skilled in the art to understand this utility model and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A protein desalting automated pre-treatment apparatus comprising a reagent carrying frame (1) and a proportioning assembly (4), characterized in that: The inner side of the reagent carrying frame (1) is clamped and connected with a reagent bottle (2), and the right side of the reagent carrying frame (1) is provided with a protection cavity (3), the proportioning assembly (4) is arranged in the protection cavity (3), and the proportioning assembly (4) comprises a supporting seat (401), a four-element gradient proportional valve (402), a pure organic pipeline (403), a pure water phase pipeline (404), an eluent pipeline (405), a proportioning and mixing pipeline (406), a flow pipe (407), a pumping pump (408), a connecting pipe (409) and a mixer (410), the supporting seat (401) is arranged in the protection cavity (3), the four-element gradient proportional valve (402) is arranged on the upper side of the supporting seat (401), the pure organic pipeline (403) is connected to the left end of the four-element gradient proportional valve (402), the pure water phase pipeline (404) is arranged at the rear end of the four-element gradient proportional valve (402), the eluent pipeline (405) is connected to the front end of the four-element gradient proportional valve (402), the proportioning and mixing pipeline (406) is arranged on the upper side of the four-element gradient proportional valve (402), the flow pipe (407) is connected to the outer end of the proportioning and mixing pipeline (406), the pumping pump (408) is arranged on the right end of the flow pipe (407), the connecting pipe (409) is connected to the right end of the pumping pump (408), and the mixer (410) is arranged at the rear end of the connecting pipe (409).

2. The automated protein desalting pre-treatment apparatus according to claim 1, wherein, The bottom of the protection cavity (3) is provided with a connecting bottom plate (5), the rear side of the upper surface of the connecting bottom plate (5) is provided with a desalination treatment machine body (6), the rear side of the desalination treatment machine body (6) is connected with a conveying pipeline (7), and the front end of the conveying pipeline (7) is connected with the rear end of the mixer (410).

3. The automated protein desalting pre-treatment apparatus of claim 2, wherein, The upper side of the desalination treatment machine body (6) is hingedly connected with a protection cover (8), the inner sides of the protection cover (8) are rotatably connected with telescopic supporting rods (9), and the lower ends of the telescopic supporting rods (9) are rotatably connected with the inner sides of the desalination treatment machine body (6).

4. The automated protein desalting pre-treatment apparatus according to claim 3, wherein, The inside of the desalination treatment machine body (6) is provided with an auxiliary assembly (10) for assisting, and the auxiliary assembly (10) comprises a servo motor (1001), a threaded screw rod (1002), a connecting piece (1003), a supporting plate (1004), a hydraulic push rod (1005) and a conveying needle (1006), the servo motor (1001) is arranged on the right side of the inside of the desalination treatment machine body (6), and the output end of the servo motor (1001) is connected with the threaded screw rod (1002) through a shaft coupling.

5. The automated protein desalting pre-treatment apparatus according to claim 4, wherein, The outer surface of the threaded screw rod (1002) is threadedly connected with the connecting piece (1003), the front side of the connecting piece (1003) is provided with the supporting plate (1004), the upper surface of the supporting plate (1004) is provided with the hydraulic push rod (1005), and the lower end of the hydraulic push rod (1005) is provided with the conveying needle (1006). The inner surface of the desalination treatment machine body (6) is provided with a sample placing plate (11).

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