Holding micro-mode biological large-scale synchronization collector
By designing a handheld miniature model organism collector that integrates a negative pressure pump and centrifuge tubes, the problems of low efficiency and poor synchronization in the synchronous collection of miniature model organism samples in existing technologies have been solved, enabling rapid and accurate large-scale sample collection and improving experimental efficiency and result accuracy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-03-06
AI Technical Summary
Existing technologies are insufficient for the rapid and precise large-scale synchronous collection of samples from tiny model organisms such as *C. elegans* and *Drosophila melanogaster*. Furthermore, existing methods can affect the synchronicity and physiological function of the samples, leading to inaccurate experimental results.
A handheld collector for large-scale synchronization of micro-model organisms was designed, integrating a negative pressure pump and centrifuge tubes. It is connected via disposable pipette tips and tubing, and uses the negative pressure pump for electric collection, reducing cross-contamination and improving efficiency.
It enables rapid and accurate large-scale sample collection, reduces operation time and synchronization differences, avoids interference with the physiological functions of samples, and improves experimental efficiency and the accuracy of results.
Smart Images
Figure CN223968514U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of experimental auxiliary equipment technology, specifically a collector for large-scale synchronization of handheld micro-model organisms. Background Technology
[0002] *C. elegans* and *Drosophila melanogaster* are two common model organisms in the life sciences. Unlike model organisms such as mice and rats, these two organisms are tiny, so researchers need to be able to rapidly collect large numbers of samples at the same developmental or adult stage for various types. Current technical solutions include:
[0003] 1. Using platinum wire to pick out individual nematodes is suitable for experiments requiring small sample sizes. A skilled researcher with over five years of experience can identify and pick out a maximum of 300 nematodes at a specific developmental stage over 30 minutes. Furthermore, the developmental time window for larval synchronization cannot be accurately determined, typically exceeding 6 hours. For adults, the time window is even more difficult to pinpoint; most studies requiring adult synchronization must use late-stage larvae instead of adults or employ drug treatment.
[0004] 2. The method of collecting by elution with buffer solution requires long-term starvation or synchronization pretreatment with drugs (drugs that inhibit the development of the reproductive system).
[0005] Similar to nematodes, collecting fruit fly larvae requires researchers to cultivate a large number of parent fruit flies in the early stages for egg laying, hatching, and collecting larvae at the same developmental stage one by one.
[0006] In existing technologies: 1. Using platinum wire to pick samples requires high speed and quality from the experimenter, is time-consuming, and results in poor synchronization between different individuals. This method is labor-intensive, slow, time-consuming, and has poor synchronization effects. It places high demands on the experimenter's skills and cannot achieve large-scale synchronized sample collection, thus hindering the progress of related research.
[0007] 2. Using buffer elution increases the error in controlling synchronization between individuals, and samples from different stages are easily mixed into the synchronized samples, contaminating the experimental results. More seriously, using long-term starvation or drugs (drugs that inhibit reproductive system development) for synchronization pretreatment can suppress the normal physiological function of the reproductive system, leading to significant changes in gene expression in the samples and affecting the research results and conclusions.
[0008] Neither of the above two technical solutions can achieve rapid and accurate synchronous collection of samples.
[0009] In view of this, the present invention provides a collector for large-scale synchronization of handheld micro-model organisms to solve the problems existing in the prior art. Utility Model Content
[0010] This invention greatly improves work efficiency by integrating a negative pressure pump and centrifuge tube into a handheld device. Therefore, this invention is completed.
[0011] This invention provides a collector for large-scale synchronization of handheld micro-model organisms, comprising a transparent shell 6 and a disposable pipette tip 1, a disposable centrifuge tube 4, and a negative pressure pump 5 disposed inside the transparent shell 6 and connected in sequence. The disposable pipette tip 1, the disposable centrifuge tube 4, and the negative pressure pump are connected by a disposable hose 2. The bottom end of the disposable centrifuge tube 4 is exposed outside the transparent shell 6 and is fixedly connected to the transparent shell 6 by a detachable cap 3. The detachable cap 3 is threadedly connected to the disposable centrifuge tube 4. The disposable centrifuge tube 4 is sealed by the detachable cap. The detachable cap 3 has two openings for fixing the disposable hose 2. The disposable hose 2 at one opening extends into the disposable centrifuge tube 4, and the disposable hose 2 at the other opening extends out of the disposable centrifuge tube 4 and is connected to the negative pressure pump 5. A switch 8 is also provided on the outer surface of the transparent shell 8, which is used to control the working state of the negative pressure pump 5.
[0012] Furthermore, the transparent outer shell 6 is also provided with a grip 7, which is made of rubber to prevent the device from slipping from the user's hand during use.
[0013] Furthermore, the transparent outer shell 6 is made of polypropylene, which facilitates the installation of internal components.
[0014] Furthermore, the outer surface of the disposable centrifuge tube 4 is also provided with scale lines for observing the capacity inside the disposable centrifuge tube 4.
[0015] Furthermore, the end of the disposable hose 2 inserted into the disposable centrifuge tube 4 is close to the bottom of the disposable centrifuge tube 4 to prevent splashing inside the disposable centrifuge tube 4 after being sucked in.
[0016] Furthermore, the negative pressure pump 5 is equipped with an internal power source to provide kinetic energy to the negative pressure pump 5.
[0017] Compared with existing technologies, the present invention provides a collector for large-scale synchronization of handheld micro-model organisms, which has the following technical advantages:
[0018] This invention provides a handheld collector for large-scale synchronization of micro-model organisms. By setting up a negative pressure pump 5 and connecting it with a pipeline, it can perform electric collection, increasing the work efficiency of operators. The collection operation can be carried out by holding the transparent shell 6, which is convenient and flexible to use. By setting up disposable tubing 2 and disposable pipette tip 1, it can realize rapid switching when collecting different samples, prevent cross-contamination between different samples and improve work efficiency.
[0019] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0021] The attached figures are labeled as follows:
[0022] 1-Disposable suction tip; 2-Disposable hose; 3-Removable cap; 4-Disposable centrifuge tube; 5-Negative pressure pump; 6-Transparent casing; 7-Grip; 8-Switch. Detailed Implementation
[0023] To further illustrate the technical means and effects adopted by this utility model in order to achieve the intended utility model purpose, the following detailed description of the specific implementation methods, structure, features and effects of this utility model is provided in conjunction with the accompanying drawings and preferred embodiments.
[0024] An embodiment of a collector for large-scale synchronization of handheld miniature model organisms
[0025] like Figure 1 As shown
[0026] A collector for large-scale synchronization of handheld micro-model organisms includes a transparent shell 6 and a disposable pipette tip 1, a disposable centrifuge tube 4, and a negative pressure pump 5, which are disposed inside the transparent shell 6 and connected in sequence. The disposable pipette tip 1, the disposable centrifuge tube 4, and the negative pressure pump are connected by a disposable hose 2. The bottom end of the disposable centrifuge tube 4 is exposed outside the transparent shell 6 and is fixedly connected to the transparent shell 6 by a removable cap 3. The removable cap 3 is threadedly connected to the disposable centrifuge tube 4. The disposable centrifuge tube 4 is sealed by the removable cap. The removable cap 3 has two openings for fixing the disposable hose 2. The disposable hose 2 at one opening extends into the disposable centrifuge tube 4, and the disposable hose 2 at the other opening extends out of the disposable centrifuge tube 4 and is connected to the negative pressure pump 5. A switch 8 is also provided on the outer surface of the transparent shell 6. The switch 8 is used to control the working state of the negative pressure pump 5.
[0027] In this embodiment, the transparent outer shell is also provided with a grip 7, which is made of rubber to prevent the device from slipping from the user's hand. The transparent outer shell 6 is made of polypropylene, which facilitates high-temperature and high-pressure sterilization of the transparent outer shell 6. The transparent outer shell 6 also facilitates the installation of components inside.
[0028] In this embodiment, the outer surface of the disposable centrifuge tube 4 is also provided with scale lines for observing the capacity inside the disposable centrifuge tube 4.
[0029] In this embodiment, the disposable hose 2 inserted into the disposable centrifuge tube 4 is located near the bottom of the disposable centrifuge tube 4 to prevent splashing inside the disposable centrifuge tube 4 after being sucked in.
[0030] In this embodiment, the negative pressure pump 5 is equipped with an internal power source to provide kinetic energy to the negative pressure pump 5.
[0031] This embodiment provides a collector for large-scale synchronization of handheld micro-model organisms, one method of which is as follows:
[0032] Depending on the specific situation, the operator adds an appropriate amount of solution required for sample collection to the disposable centrifuge tube 4 and assembles all components of the device. Then, the biological sample to be collected is placed under a microscope, and the device is held with one hand for selection and collection. The status of the negative pressure pump 5 is controlled by switch 8.
[0033] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to a preferred embodiment, it is not intended to limit the present utility model. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present utility model. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model shall still fall within the scope of the present utility model.
Claims
1. A collector for large-scale synchronization of gripped microorganisms, characterized in that: The utility model relates to a kind of centrifuge tube, including transparent shell (6) and be arranged in the inside of transparent shell (6) and connect in turn disposable suction head (1), disposable centrifuge tube (4) and negative pressure pump (5), the disposable suction head (1), disposable centrifuge tube (4) and negative pressure pump between by disposable hose (2) connection, the bottom end of the disposable centrifuge tube (4) is exposed in the outside of the transparent shell (6), by detachable pipe cap (3) and the transparent shell (6) fixed connection, the detachable pipe cap (3) is screwed with the disposable centrifuge tube (4);The disposable centrifuge tube (4) is sealed by the detachable pipe cap, the detachable pipe cap (3) is provided with two openings, for fixing disposable hose (2), one disposable hose (2) in one opening enters the disposable centrifuge tube (4) inside, another disposable hose (2) is connected outside the disposable centrifuge tube (4) in another opening, and is connected with the negative pressure pump (5), switch (8) is further provided on the outer surface of the transparent shell (6), and the switch (8) is used to control the working condition of negative pressure pump (5).
2. The holder for large-scale synchronization of microorganisms according to claim 1, characterized in that: The transparent shell is further provided with a holding portion (7), and the holding portion (7) is made of rubber.
3. The holder for large-scale synchronization of microorganisms according to claim 1, wherein: The disposable centrifuge tube (4) is further provided with a scale line on the outer surface.
4. The holder for large-scale synchronization of microorganisms according to claim 1, wherein: The disposable hose (2) connected to the disposable centrifuge tube (4) is close to the bottom of the disposable centrifuge tube (4).
5. The holder for large-scale synchronization of microorganisms according to claim 1, wherein: The negative pressure pump (5) is provided with an internal power supply.