Centrifugal ultrafiltration equipment for exosome extraction and separation
By using a multidimensional centrifugation system and intelligent temperature control technology, the problems of centrifugation deviation and insufficient temperature control in existing equipment have been solved, achieving efficient and low-pollution exosome extraction and adapting to sample needs in multiple scenarios.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-16
- Publication Date
- 2026-03-10
AI Technical Summary
Existing exosome extraction equipment is prone to deviation during high-speed rotation, has difficulty in dynamically adjusting centrifugation parameters, and suffers from poor temperature control, which affects centrifugation efficiency and sample purity.
It adopts a multi-dimensional centrifugal system that combines electromagnetic repulsion with mechanical centrifugation. The centrifugal angle is adjustable from 0 to 90° by using the magnetic repulsion between the limiting electromagnet and the rotating ring. It is also equipped with an intelligent temperature control system that integrates dual-cycle heat dissipation and adjusts the magnetic field strength and rotation speed in real time.
It achieves efficient and low-pollution multi-angle centrifugation, ensuring sample purity and activity, and controlling the temperature difference within ±2℃ to adapt to different sample characteristics.
Smart Images
Figure CN223980609U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biopharmaceutical technology, specifically to a centrifugal ultrafiltration device for exosome extraction and separation. Background Technology
[0002] Exosomes, as key carriers of intercellular communication, require efficient and high-purity separation techniques, which are fundamental to biomedical research. Current exosome extraction techniques primarily rely on ultracentrifugation, density gradient centrifugation, and ultrafiltration. However, these methods face significant technical bottlenecks in practical applications, specifically: Existing centrifugation equipment often employs unidirectional fixed-angle centrifugation, which can easily cause the centrifuge tube to shift during high-speed rotation, requiring external clamping structures (such as movable beads and clamps) to maintain stability. However, mechanical friction can affect centrifugation efficiency and increase the risk of sample contamination. Fixed centrifugation angles (e.g., horizontal or vertical) make it difficult to dynamically adjust centrifugation parameters based on different sample characteristics (e.g., viscosity, particle distribution), leading to impurity residues or exosome loss. Traditional centrifugation equipment lacks efficient temperature control systems, and prolonged high-speed operation can easily cause sample denaturation due to heat. Some equipment uses a single cooling fan or passive cooling, resulting in large temperature fluctuations (often exceeding ±5℃), affecting exosome activity. While existing technologies may already address these issues, this paper aims to provide an alternative or replacement solution. Utility Model Content
[0003] To achieve the above objectives, this utility model is implemented through the following technical solution: a centrifugal ultrafiltration device for exosome extraction and separation, comprising: a centrifuge chamber, a centrifugal structure, and a cooling structure, wherein the centrifugal structure and the cooling structure are installed inside the centrifuge chamber, and the centrifugal structure includes: a rotary drive motor, a centrifugal ring, a limiting ring metal strip, a limiting electromagnet, and multiple centrifugal limiting components;
[0004] The rotary drive is installed inside the centrifuge chamber. The centrifugal ring is inserted into the inside of the centrifuge chamber via a bearing and is connected to the drive end of the rotary drive. Multiple centrifugal limiting components are installed on the centrifugal ring. The limiting ring metal strip is installed inside the centrifuge chamber. The limiting electromagnet is installed on the limiting ring metal strip.
[0005] The centrifugal limiting assembly includes: a concave limiting block, a fitted ring, a rotating ring, a pair of limiting shafts, a pair of angular rotating shafts, a pair of arc sliders, and an arc magnet;
[0006] The concave limiting blocks are evenly inserted into the centrifugal ring. The sleeve ring is inserted into the concave limiting blocks through a pair of limiting shafts. The concave limiting blocks are provided with a pair of arc-shaped convex grooves. A pair of arc-shaped sliders are respectively movably inserted into the inner side of the pair of arc-shaped convex grooves. A pair of angle rotation shafts are respectively inserted into both sides of the rotating ring, and the pair of angle rotation shafts are respectively connected to the pair of arc-shaped sliders. The arc magnet is installed on the rotating ring.
[0007] It should be noted that, as described above, the operation of the rotary drive motor drives the centrifugal ring on the drive end of the rotary drive motor to rotate. The centrifugal ring rotates stably along the inner side of the centrifuge chamber, and drives multiple concave limiting blocks on it to rotate centrifugally. Through centrifugal force, the test tubes between the set ring and the rotating ring generate centrifugal force due to the centrifugal rotation, causing the rotating ring to drive a pair of angled rotating shafts on it. The pair of angled rotating shafts drive the arc-shaped sliders on them respectively. Through centrifugation, the limiting electromagnets on the limiting ring metal strips are energized, causing the limiting ring metal strips to become magnetic. The magnetism on the limiting ring metal strips magnetically repels the arc-shaped magnets on the rotating ring, so that the test tubes inside the concave limiting blocks can both rotate centrifugally and rotate by insertion and lifting repulsion, thereby performing multi-angle centrifugal sieving of the test tubes.
[0008] Preferably, the cooling structure includes: a cooler, a radiator, a cooling fan, and a cooling fan;
[0009] The cooler is installed inside the centrifuge chamber, the radiator is installed outside the centrifuge chamber and connected to the cooler, the cooling fan is installed on the radiator, and the cooling fan is installed on the cooler.
[0010] It should be noted that, as described above, the inside of the centrifuge is cooled by a cooler, and air is drawn around the cooler by a cooling fan. The cooler absorbs the heat, the radiator dissipates the heat, and the cooling fan quickly dissipates the heat from the radiator.
[0011] Preferably, the limiting electromagnet is equipped with a current regulator and a resistance regulator.
[0012] Preferably, a temperature sensor is provided on the inside of the centrifuge.
[0013] Preferably, both the fitted ring and the rotating ring are equipped with sealing rubber rings.
[0014] Preferably, the inner side of several of the arc-shaped grooves is provided with arc-shaped limiting shafts, and the several arc-shaped limiting shafts are respectively movably inserted into several of the arc-shaped sliders. Beneficial effects
[0015] This invention provides a centrifugal ultrafiltration device for exosome extraction and separation. Compared with existing technologies, this centrifugal ultrafiltration device for exosome extraction and separation offers the following advantages: The multidimensional centrifugation system employs a combination of electromagnetic repulsion and mechanical centrifugation. Through the dynamic repulsion between the limiting electromagnet and the rotating circular magnetic poles, combined with the arc trajectory adjustment of the angle rotation axis and the arc slider, the test tube can achieve an adjustable centrifugation angle of 0-90° during high-speed rotation. Combined with the elastic sealing structure of the centrifugation limiting component, it ensures centrifugation efficiency while preventing sample contamination. The intelligent temperature control system integrates dual-cycle heat dissipation. The temperature sensor provides real-time feedback, and the gradient heat dissipation architecture of cooler-radiator-dual fan accelerates refrigerant circulation through forced convection by the cooling fan. The cooling fan adopts a biomimetic impeller design to improve heat exchange efficiency by 30%, ensuring a continuous operating temperature difference of ≤±2℃. The electromagnetic adjustment system is equipped with current-resistance dual-parameter control, which can adjust the magnetic field strength and centrifugation speed in real time according to different sample characteristics, achieving multi-scenario adaptation from DNA extraction to cell sorting. This device breaks through the limitations of traditional centrifugal equipment's unidirectional centrifugation and pioneers a new paradigm that combines multidimensional dynamic centrifugation with intelligent temperature control. Attached Figure Description
[0016] Figure 1 This is a front cross-sectional view of a centrifugal ultrafiltration device for exosome extraction and separation according to the present invention.
[0017] Figure 2 This is a top cross-sectional view of a centrifugal ultrafiltration device for exosome extraction and separation according to the present invention.
[0018] Figure 3 This is a schematic diagram of the centrifugal structure of a centrifugal ultrafiltration device for exosome extraction and separation according to the present invention.
[0019] In the diagram: 1. Centrifuge; 2. Rotary drive motor; 3. Centrifugal ring; 4. Limiting ring metal strip; 5. Limiting electromagnet; 6. Concave limiting block; 7. Set ring; 8. Rotating ring; 9. Limiting shaft; 10. Angle rotation shaft; 11. Arc slider; 12. Arc magnet; 13. Cooler; 14. Radiator; 15. Cooling fan; 16. Cooling fan. Detailed Implementation
[0020] Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.
[0021] Those skilled in the art should connect all electrical components and their compatible power supplies in this case via wires. Appropriate controllers and encoders should be selected according to the actual situation to meet control requirements. The specific connection and control sequence should refer to the working principle described below, where the electrical components are connected in sequence. The detailed connection methods are well-known in the art. The following mainly introduces the working principle and process, and will not describe the electrical control further. Example
[0022] The present invention will now be described in detail with reference to the accompanying drawings, such as... Figure 1-3 As shown, the centrifugal structure and the cooling structure are installed inside the centrifuge chamber 1. The centrifugal structure includes: a rotary drive motor 2, a centrifugal ring 3, a limiting ring metal strip 4, a limiting electromagnet 5, and multiple centrifugal limiting components. The rotary drive motor 2 is installed inside the centrifuge chamber 1. The centrifugal ring 3 is inserted into the inside of the centrifuge chamber 1 via bearings, and the centrifugal ring 3 is connected to the drive end of the rotary drive motor 2. Multiple centrifugal limiting components are installed on the centrifugal ring 3, and the limiting ring metal strip... 4 is installed inside the centrifuge chamber 1, and the limiting electromagnet 5 is installed on the limiting ring metal strip 4; the centrifugal limiting assembly includes: a concave limiting block 6, a fitted ring 7, a rotating ring 8, a pair of limiting shafts 9, a pair of angular rotating shafts 10, a pair of arc-shaped sliders 11, and an arc-shaped magnet 12; the concave limiting block 6 is evenly inserted into the centrifugal ring 3, and the fitted ring 7 is inserted into the concave limiting block 6 through a pair of limiting shafts 9; the concave limiting block 6 has a pair of arc-shaped convex grooves, and the pair of arc-shaped sliders 9 are installed on the inner side of the centrifuge chamber 1. Arc-shaped sliders 11 are movably inserted into the inner sides of a pair of arc-shaped convex grooves, and a pair of angle rotation shafts 10 are respectively inserted into the two sides of the rotating ring 8, and the pair of angle rotation shafts 10 are respectively connected to the pair of arc-shaped sliders 11. Arc-shaped magnets 12 are mounted on the rotating ring 8. The cooling structure includes: a cooler 13, a radiator 14, a cooling fan 15, and a cooling fan 16. The cooler 13 is mounted inside the centrifuge chamber 1, and the radiator 14 is mounted outside the centrifuge chamber 1. The radiator 14 is connected to the cooler 13, the cooling fan 15 is mounted on the radiator 14, and the cooling fan 16 is mounted on the cooler 13; the limiting electromagnet 5 is provided with a current regulator and a resistance regulator; a temperature sensor is provided on the inner side of the centrifuge 1; the sleeve ring 7 and the rotating ring 8 are respectively sealed with rubber rings; the inner side of several arc-shaped grooves is provided with arc-shaped limiting shafts 9, and several arc-shaped limiting shafts 9 are respectively movably inserted into several arc-shaped sliders 11.
[0023] According to the appendix Figure 1-3It is concluded that the operation of the rotary drive 2 drives the centrifugal ring 3 on the drive end of the rotary drive 2 to rotate. The centrifugal ring 3 rotates stably along the inner side of the centrifuge chamber 1. The centrifugal ring 3 drives the multiple concave limiting blocks 6 on it to rotate centrifugally. Through centrifugal force, the test tube between the set ring 7 and the rotating ring 8 generates centrifugal force due to the centrifugal rotation. This causes the rotating ring 8 to drive a pair of angled rotation shafts 10 on it. The pair of angled rotation shafts 10 drive the arc sliders 11 on them respectively. Through centrifugation, the limiting electromagnets 5 on the limiting ring metal strips 4 are energized. The limiting ring metal strip 4 becomes magnetic, and the magnetism on the limiting ring metal strip 4 magnetically repels the arc magnet 12 on the rotating ring 8, so that the test tube inside the concave limiting block 6 can be centrifugally rotated or inserted and lifted to repel rotation, thereby centrifuging and sieving the test tube at multiple angles; the inside of the centrifuge box 1 is cooled by the cooler 13, and at the same time, the air is drawn to the area around the cooler 13 by the cooling fan 16, the cooler 13 absorbs the heat, the heat is dissipated by the radiator 14, and the heat is quickly dissipated by the cooling fan 15.
[0024] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A centrifugal ultrafiltration apparatus for exosome extraction and isolation, comprising: Centrifugal box, centrifugal structure and cooling structure are installed on the inner side of the centrifugal box, characterized in that the centrifugal structure comprises a rotating drive machine, a centrifugal ring, a limiting ring metal strip, a limiting electromagnet and a plurality of centrifugal limiting components. The rotating drive machine is installed on the inner side of the centrifugal box, the centrifugal ring is inserted into the inner side of the centrifugal box through a bearing, and the centrifugal ring is connected to the driving end of the rotating drive machine, a plurality of centrifugal limiting components are installed on the centrifugal ring, the limiting ring metal strip is installed on the inner side of the centrifugal box, and the limiting electromagnet is installed on the limiting ring metal strip. The centrifugal limiting component comprises a concave limiting block, a sleeving ring, a rotating ring, a pair of limiting shafts, a pair of angle rotating shafts, a pair of arc sliders and an arc magnet. The concave limiting block is uniformly inserted into the centrifugal ring, the sleeving ring is inserted into the concave limiting block through a pair of limiting shafts, a pair of arc convex grooves are formed in the concave limiting block, a pair of arc sliders are movably inserted into the inner side of a pair of arc convex grooves, a pair of angle rotating shafts are inserted into the two sides of the rotating ring, and a pair of angle rotating shafts are connected to a pair of arc sliders, and the arc magnet is installed on the rotating ring.
2. The centrifugal ultrafiltration apparatus for exosome extraction and separation according to claim 1, characterized in that, The cooling structure comprises a cooler, a radiator, a heat dissipation fan and a cooling fan. The cooler is installed on the inner side of the centrifugal box, the radiator is installed on the outer side of the centrifugal box, and the radiator is connected to the cooler, the heat dissipation fan is installed on the radiator, and the cooling fan is installed on the cooler.
3. The centrifugal ultrafiltration apparatus for exosome extraction and separation according to claim 2, characterized in that, The limiting electromagnet is provided with a current regulator and a resistance regulator.
4. The centrifugal ultrafiltration apparatus for exosome extraction and separation according to claim 3, characterized in that, The inner side of the centrifugal box is provided with a temperature sensor.
5. The centrifugal ultrafiltration apparatus for exosome extraction and separation according to claim 4, characterized in that, The sleeving ring and the rotating ring are respectively sealed with rubber rings.
6. The centrifugal ultrafiltration apparatus for exosome extraction and separation according to claim 5, characterized in that, A plurality of arc limiting shafts are arranged on the inner side of the arc convex groove, and a plurality of arc limiting shafts are movably inserted into a plurality of arc sliders.