Extrusion preparation device for extrusion vesicles

Abstract

The utility model belongs to the technical field of medical instruments, and particularly relates to an extrusion preparation device for extruded vesicles, which comprises a shell, a first piston is hermetically and slidably connected to one end of the shell, and a second piston is hermetically and slidably connected to the other end of the shell; the filter assembly is detachably installed in the middle of the shell, the interior of the shell is divided into a sample injection cavity and a filter cavity by the filter assembly, the sample injection cavity is located between the filter assembly and the second piston, and the filter cavity is located between the filter assembly and the first piston; the sample injection port is formed in the shell and communicated with the sample injection cavity, and the sample injection port is used for adding a sample before filtration into the sample injection cavity; the sample outlet is formed in the shell and communicated with the filtering cavity, and the sample outlet is used for discharging the filtered sample in the filtering cavity; and the size of the filter component is matched with the sizes of the sample injection cavity and the filter cavity. The vesicle preparation device can improve the vesicle preparation efficiency and prevent samples from being polluted.

Description

Technical Field

[0001] This utility model belongs to the field of medical device technology, and in particular relates to an extrusion preparation device for extruded vesicles. Background Technology

[0002] The extrusion method for preparing engineered vesicles involves passing cells through a large-pore filter membrane and then separating them into vesicles of the desired diameter via ultracentrifugation. Currently, laboratory equipment for preparing extruded vesicles consists of two dedicated, airtight syringes and a filter membrane fixing device in the middle. However, due to the small diameter of the syringe needle holes, the filter membrane at the syringe needle position experiences greater pressure than the filter membrane at other positions. When pushing samples with high viscosity or small filter membrane pore diameters, the resistance is high, and excessive force during the pushing process can cause filter membrane damage. Once the filter membrane is damaged, it will lead to sample contamination, and the extruder must be disassembled and the filter membrane reinstalled for extrusion again, which is not only time-consuming and labor-intensive but also prone to sample contamination.

[0003] Therefore, it is necessary to design an extrusion preparation device for extruded vesicles to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide an extrusion preparation device for extruded vesicles to solve the above-mentioned problems and achieve the goal of improving vesicle preparation efficiency and preventing sample contamination.

[0005] To achieve the above objectives, this utility model provides the following solution: an extrusion preparation apparatus for extruded vesicles, comprising...

[0006] The outer casing has a first piston that is sealed and slidably connected to one end, and a second piston that is sealed and slidably connected to the other end of the outer casing;

[0007] A filter assembly is detachably installed in the middle of the housing. The filter assembly divides the interior of the housing into a sample inlet chamber and a filter chamber. The sample inlet chamber is located between the filter assembly and the second piston, and the filter chamber is located between the filter assembly and the first piston.

[0008] An inlet is provided on the outer shell and communicates with the inlet chamber. The inlet is used to add the unfiltered sample into the inlet chamber.

[0009] A sample outlet is provided on the outer shell and communicates with the filter chamber. The sample outlet is used to discharge the filtered sample from the filter chamber.

[0010] The dimensions of the filter assembly are matched to the dimensions of the sample inlet chamber and the filter chamber.

[0011] According to the present invention, an extrusion preparation device for extruded vesicles is provided. The filter assembly includes a fixed shell, which is U-shaped. The inner wall size of the fixed shell is adapted to the outer wall size of the outer shell. A filter part is fixedly connected to the middle of the inner wall of the fixed shell, and the filter part is sealed and snapped into the middle of the outer shell.

[0012] According to the present invention, an extrusion preparation device for extruded vesicles is provided. The outer shell includes a first outer shell section and a second outer shell section fixedly connected. The filter chamber is opened in the first outer shell section, and the sample inlet chamber is opened in the sample inlet chamber. The ends of the first outer shell section and the second outer shell section that are close to each other are provided with sealing ring grooves. A gap is left between the first outer shell section and the second outer shell section, and the filter part is sealed and snapped into the gap.

[0013] According to this utility model, an extrusion preparation device for extruded vesicles is provided. The filter section includes a fixed base, which is fixedly connected to the middle of the inner sidewall of a fixed shell. A central hole is opened inside the fixed shell, and a filter membrane is fixedly connected to the inner sidewall of the central hole. The size of the filter membrane is adapted to the size of the filter cavity and the sample injection cavity. Sealing rings are fixedly connected to both side walls of the fixed shell. The sealing rings are coaxial with the central hole and are adapted to the sealing ring groove.

[0014] According to the present invention, an extrusion preparation device for extruded vesicles is provided, wherein the first piston is slidably disposed in the filter chamber, the end of the first piston located inside the filter chamber is fixedly connected to a first sealing part, and the end of the first piston located outside the filter chamber is fixedly connected to a first pusher plate.

[0015] According to the present invention, an extrusion preparation device for extruded vesicles is provided. The first sealing part includes a first limiting ring and a first retaining ring. The first limiting ring is fixedly sleeved on the outer side of the end of the first piston. The first retaining ring is fixedly disposed on the inner side wall of the end of the filter chamber away from the sample inlet chamber. The outer side wall of the first limiting ring is in sealing sliding contact with the inner side wall of the filter chamber, and the inner side wall of the first retaining ring is in sealing sliding contact with the outer side wall of the first piston.

[0016] According to the present invention, an extrusion preparation device for extruded vesicles is provided, wherein the second piston is slidably disposed in the injection chamber, the end of the second piston located inside the injection chamber is fixedly connected to a second sealing part, and the end of the second piston located outside the injection chamber is fixedly connected to a second pusher plate.

[0017] According to the present invention, an extrusion preparation device for extruded vesicles is provided. The second sealing part includes a second limiting ring and a second retaining ring. The second limiting ring is fixedly sleeved on the outer side of the end of the second piston. The second retaining ring is fixedly disposed on the inner side wall of the end of the sample inlet chamber away from the filter chamber. The outer side wall of the second limiting ring is in sealing sliding contact with the inner side wall of the sample inlet chamber, and the inner side wall of the second retaining ring is in sealing sliding contact with the outer side wall of the second piston.

[0018] According to the present invention, an extrusion preparation device for extruded vesicles is provided, wherein a sample outlet plug is detachably provided in the sample outlet and a sample inlet plug is detachably provided in the sample inlet.

[0019] Compared with the prior art, the present invention has the following advantages and technical effects:

[0020] This invention solves the problem of filter membrane damage caused by high pressure at the syringe needle hole in the prior art by designing the dimensions of the sample inlet chamber and filter chamber of the outer shell to match the dimensions of the filter assembly. During the extrusion filtration process, the filter membrane will not be damaged due to high pressure at a certain point, thus avoiding sample contamination and improving the extrusion preparation efficiency of vesicles. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly described below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the entire utility model. Figure 1 ;

[0023] Figure 2 This is a schematic diagram of the entire utility model. Figure 2 ;

[0024] Figure 3 This is a schematic diagram of the outer shell of this utility model;

[0025] Figure 4 This is a schematic diagram of the filter section of this utility model;

[0026] Figure 5 This is a schematic diagram of the first piston of this utility model;

[0027] Figure 6 This is a schematic diagram of the second piston of this utility model.

[0028] The components are as follows: 1. Outer shell; 101. First outer shell section; 102. Second outer shell section; 103. Filter chamber; 104. Sample inlet chamber; 105. Sample outlet; 106. Sample inlet; 107. Sealing ring groove; 108. Gap; 2. First piston; 3. First push plate; 4. First limiting ring; 5. Second piston; 6. Second push plate; 7. Sample inlet plug; 8. Fixed shell; 9. Sample outlet plug; 10. Second limiting ring; 11. Fixed base; 12. Central hole; 13. Filter membrane; 14. Sealing ring; 15. First retaining ring; 16. Second retaining ring. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0031] Reference Figures 1 to 6 As shown, this utility model provides an extrusion preparation apparatus for extruded vesicles, including...

[0032] The outer casing 1 has a first piston 2 sealed and slidably connected to one end, and a second piston 5 sealed and slidably connected to the other end of the outer casing 1.

[0033] The filter assembly is detachably installed in the middle of the housing 1. The filter assembly divides the interior of the housing 1 into a sample inlet chamber 104 and a filter chamber 103. The sample inlet chamber 104 is located between the filter assembly and the second piston 5, and the filter chamber 103 is located between the filter assembly and the first piston 2.

[0034] The inlet 106 is located on the outer shell 1 and communicates with the inlet chamber 104. The inlet 106 is used to add the sample before filtration into the inlet chamber 104.

[0035] The sample outlet 105 is located on the outer shell 1 and communicates with the filter chamber 103. The sample outlet 105 is used to discharge the filtered sample from the filter chamber 103.

[0036] The dimensions of the filter assembly are matched with the dimensions of the injection chamber 104 and the filter chamber 103.

[0037] By designing the dimensions of the sample inlet chamber 104 and the filter chamber 103 of the outer shell 1 to match the dimensions of the filter assembly, the problem of filter membrane damage caused by high pressure at the syringe needle hole position in the prior art can be solved. During the extrusion filtration process, the filter membrane will not be damaged due to high pressure at a certain point, and the problem of sample contamination will not occur, thereby improving the extrusion preparation efficiency of vesicles.

[0038] Furthermore, the filter assembly includes a fixed shell 8, which is U-shaped. The inner wall size of the fixed shell 8 is adapted to the outer wall size of the outer shell 1. A filter part is fixedly connected to the middle of the inner wall of the fixed shell 8, and the filter part is sealed and snapped into the middle of the outer shell 1.

[0039] Furthermore, the outer shell 1 includes a first outer shell section 101 and a second outer shell section 102 that are fixedly connected. A filter chamber 103 is opened in the first outer shell section 101, and a sample inlet chamber 104 is opened in the sample inlet chamber 104. Sealing ring grooves 107 are opened at the ends of the first outer shell section 101 and the second outer shell section 102 that are close to each other. A gap 108 is left between the first outer shell section 101 and the second outer shell section 102, and the filter part is sealed and snapped into the gap 108.

[0040] Furthermore, the filtration unit includes a fixing seat 11, which is fixedly connected to the middle of the inner wall of the fixing shell 8. A central hole 12 is opened inside the fixing shell 8, and a filter membrane 13 is fixedly connected to the inner wall of the central hole 12. The size of the filter membrane 13 is adapted to the size of the filter chamber 103 and the sample inlet chamber 104. Sealing rings 14 are fixedly connected to both sides of the fixing shell 8. The sealing rings 14 are coaxial with the central hole 12 and are adapted to the sealing ring groove 107.

[0041] After the fixing seat 11 is inserted into the gap 108, the sealing rings 14 on both sides of the fixing seat 11 are engaged in the sealing ring groove 107 to form a sealing effect, preventing the sample in the filter chamber 103 and the sample injection chamber 104 from leaking.

[0042] Furthermore, the first piston 2 is slidably disposed inside the filter chamber 103, with a first sealing part fixedly connected to one end of the first piston 2 inside the filter chamber 103, and a first push plate 3 fixedly connected to one end of the first piston 2 outside the filter chamber 103.

[0043] Furthermore, the first sealing part includes a first limiting ring 4 and a first retaining ring 15. The first limiting ring 4 is fixedly sleeved on the outer side of the end of the first piston 2, and the first retaining ring 15 is fixedly disposed on the inner side wall of the end of the filter chamber 103 away from the sample injection chamber 104. The outer side wall of the first limiting ring 4 is in sealing sliding contact with the inner side wall of the filter chamber 103, and the inner side wall of the first retaining ring 15 is in sealing sliding contact with the outer side wall of the first piston 2.

[0044] The first limiting ring 4 and the first retaining ring 15 not only seal the inside of the filter chamber 103, but also prevent the first piston 2 from being pulled out of the filter chamber 103.

[0045] Furthermore, the second piston 5 is slidably disposed in the injection chamber 104, and a second sealing part is fixedly connected to one end of the second piston 5 inside the injection chamber 104, while a second pusher 6 is fixedly connected to one end of the second piston 5 outside the injection chamber 104.

[0046] Furthermore, the second sealing part includes a second limiting ring 10 and a second retaining ring 16. The second limiting ring 10 is fixedly sleeved on the outer side of the end of the second piston 5, and the second retaining ring 16 is fixedly disposed on the inner side wall of the end of the sample injection chamber 104 away from the filter chamber 103. The outer side wall of the second limiting ring 10 is in sealing sliding contact with the inner side wall of the sample injection chamber 104, and the inner side wall of the second retaining ring 16 is in sealing sliding contact with the outer side wall of the second piston 5.

[0047] The second limiting ring 10 and the second retaining ring 16 not only seal the inside of the injection chamber 104, but also prevent the second piston 5 from being pulled out of the injection chamber 104.

[0048] Furthermore, a sample outlet plug 9 is detachably installed inside the sample outlet 105, and a sample inlet plug 7 is detachably installed inside the sample inlet 106.

[0049] The working process of this utility model is as follows:

[0050] First, the fixing seat 11 is installed in the gap 108 of the outer shell 1 through the fixing shell 8. The sealing ring 14 and the sealing ring groove 107 form a sealed environment between the filter chamber 103 and the sample inlet chamber 104. Then, the sample inlet plug 7 is opened, and the sample is injected into the sample inlet chamber 104 through the sample inlet 106. After that, the sample inlet 106 is sealed again with the sample inlet plug 7. The second piston 5 is pushed by the second pusher 6 to apply a squeezing force to the sample in the sample inlet chamber 104. Under the action of the squeezing force, the sample passes through the filter membrane 13 and enters the filter chamber 103. The sample outlet plug 9 is opened, and the squeezed sample is discharged from the filter chamber 103 through the sample outlet 105 and collected for use.

[0051] In the description of this utility model, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0052] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Various modifications and improvements to the technical solutions of the present utility model made by those skilled in the art without departing from the spirit of the present utility model should fall within the protection scope of the present utility model.

Claims

1. An extrusion device for the extrusion production of extruded vesicles, characterized in that Comprising A housing (1) is sealed and slidably connected with a first piston (2) at one end, and sealed and slidably connected with a second piston (5) at the other end; A filter assembly is detachably installed in the middle of the housing (1), which separates the inside of the housing (1) into a sample inlet cavity (104) and a filter cavity (103), the sample inlet cavity (104) is located between the filter assembly and the second piston (5), and the filter cavity (103) is located between the filter assembly and the first piston (2); A sample inlet (106) is provided on the housing (1) and communicates with the sample inlet cavity (104), which is used to add unfiltered samples into the sample inlet cavity (104); A sample outlet (105) is provided on the housing (1) and communicates with the filter cavity (103), which is used to discharge filtered samples from the filter cavity (103); The size of the filter assembly matches the size of the sample inlet cavity (104) and the filter cavity (103).

2. An extrusion device for the production of extruded vesicles according to claim 1, characterized in that The filter assembly includes a fixed shell (8), which is U-shaped, the inner side wall size of the fixed shell (8) matches the outer side wall size of the housing (1), and a filter part is fixedly connected to the middle of the inner side wall of the fixed shell (8), which is sealingly connected in the middle of the housing (1).

3. An extrusion device for the production of extruded vesicles according to claim 2, characterized in that The housing (1) includes a first housing segment (101) and a second housing segment (102) fixedly connected, the filter cavity (103) is provided in the first housing segment (101), the sample inlet cavity (104) is provided in the sample inlet cavity (104), and the end of the first housing segment (101) and the second housing segment (102) close to each other is provided with a sealing ring groove (107), and a gap (108) is left between the first housing segment (101) and the second housing segment (102), and the filter part is sealingly connected in the gap (108).

4. An extrusion device for the production of extruded vesicles according to claim 3, characterized in that The filter part includes a fixed seat (11) fixedly connected to the middle of the inner side wall of the fixed shell (8), a center hole (12) is provided in the fixed shell (8), a filter membrane (13) is fixedly connected to the inner side wall of the center hole (12), the size of the filter membrane (13) matches the size of the filter cavity (103) and the sample inlet cavity (104), and a sealing ring (14) is fixedly connected to the two side walls of the fixed shell (8), the sealing ring (14) is coaxial with the center hole (12), and the sealing ring (14) matches the sealing ring groove (107).

5. An apparatus for the extrusion production of extruded vesicles according to claim 1, wherein The first piston (2) is slidably arranged in the filter cavity (103), and a first sealing part is fixedly connected to one end of the first piston (2) inside the filter cavity (103), and a first push disc (3) is fixedly connected to the other end of the first piston (2) outside the filter cavity (103).

6. An extrusion device for the production of extruded vesicles according to claim 5, characterized in that The first sealing part comprises a first limiting ring (4) and a first clamping ring (15), the first limiting ring (4) is fixedly sleeved outside the end of the first piston (2), the first clamping ring (15) is fixedly arranged on the inner side wall of the end of the filter cavity (103) away from the sample inlet cavity (104), the outer side wall of the first limiting ring (4) is in sealing sliding contact with the inner side wall of the filter cavity (103), and the inner side wall of the first clamping ring (15) is in sealing sliding contact with the outer side wall of the first piston (2).

7. An apparatus for the extrusion production of extruded vesicles according to claim 1, wherein The second piston (5) is slidably arranged in the sample inlet cavity (104), and the second piston (5) is fixedly connected with a second sealing part at one end inside the sample inlet cavity (104) and is fixedly connected with a second push disc (6) at one end outside the sample inlet cavity (104).

8. An extrusion device for the production of extruded vesicles according to claim 7, characterized in that The second sealing part comprises a second limiting ring (10) and a second clamping ring (16), the second limiting ring (10) is fixedly sleeved outside the end of the second piston (5), and the second clamping ring (16) is fixedly arranged on the inner side wall of the end of the sample inlet cavity (104) away from the filter cavity (103); the outer side wall of the second limiting ring (10) is in sealing sliding contact with the inner side wall of the sample inlet cavity (104), and the inner side wall of the second clamping ring (16) is in sealing sliding contact with the outer side wall of the second piston (5).

9. An apparatus for the extrusion production of extruded vesicles according to claim 1, wherein, A sample outlet plug (9) is detachably arranged in the sample outlet (105), and a sample inlet plug (7) is detachably arranged in the sample inlet (106).

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