Nucleic acid detection card box and system
By setting up a flow-blocking section and a flow-blocking cavity in the nucleic acid test cartridge, the problem of air bubbles being trapped in the sample solution is solved by utilizing the buoyancy of air bubbles to expel the gas, thus achieving a full reaction of the sample solution in the reaction chamber and accurate test results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-01
- Publication Date
- 2026-03-10
Smart Images
Figure CN223983648U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of in vitro medical diagnostic devices, in particular to a nucleic acid detection cartridge and system. BACKGROUND
[0002] As a means of in vitro nucleic acid detection, the nucleic acid detection cartridge has the characteristics of small reaction volume and saving reagent consumption, and has wide application value. The nucleic acid detection technology includes sample liquid processing and subsequent PCR (polymerase chain reaction, also known as in vitro DNA amplification technology) and optical detection steps.
[0003] The existing nucleic acid detection cartridge generally first injects sample liquid into the reaction chamber of the cartridge. After the sample liquid reacts with the reaction reagent in the reaction chamber to form a detection liquid, the detection result is obtained by optical detection analysis of the detection liquid in the reaction chamber. In the process of injecting sample liquid into the reaction chamber, air bubbles may be trapped in the sample liquid, resulting in air bubbles in the detection liquid formed in the reaction chamber, which will cause the reaction chamber to be unable to be filled with the detection liquid, thereby affecting the accuracy of the detection result. Practical new type content
[0004] In view of the above problems, the embodiments of the present application provide a nucleic acid detection cartridge and system, which can improve the accuracy of the detection result.
[0005] According to an aspect of the embodiments of the present application, a nucleic acid detection cartridge is provided, which is provided with a sample processing chamber, a first reaction chamber and a second reaction chamber. The sample processing chamber is in communication with the first reaction chamber and the second reaction chamber through a liquid injection channel on the cartridge. The liquid injection channel includes a main channel, a first branch channel and a second branch channel. The input end of the main channel is in communication with the sample processing chamber. The first branch channel is in communication between the output end of the main channel and the first reaction chamber. The second branch channel is in communication between the output end of the main channel and the second reaction chamber. The second branch channel has a flow resistance section in communication with the output end of the main channel and extending upward. The flow resistance section is provided with a flow resistance cavity with an increasing cross-sectional area.
[0006] In an optional manner, the cartridge is provided with an air hole in communication with the sample processing chamber. The air hole is used to drive the sample liquid in the sample processing chamber to be injected into the first reaction chamber and the second reaction chamber through the liquid injection channel when a positive pressure is applied.
[0007] In one alternative embodiment, the cartridge also includes a waste liquid chamber. The input end of the first reaction chamber is connected to the first branch flow channel, and the output end of the first reaction chamber is connected to the waste liquid chamber through a flow channel. The input end of the second reaction chamber is connected to the second branch flow channel, and the output end of the second reaction chamber is connected to the waste liquid chamber through a flow channel. A water-proof and gas-permeable membrane is provided in the flow channel between the output end of the first reaction chamber, the output end of the second reaction chamber, and the waste liquid chamber. The water-proof and gas-permeable membrane is used to block the sample liquid and allow the gas in the sample liquid to be discharged into the waste liquid chamber.
[0008] In one alternative embodiment, the flow channels between the output end of the first reaction chamber and the water-proof and breathable membrane, as well as the flow channels between the output end of the second reaction chamber and the water-proof and breathable membrane, are both connected to an exhaust flow channel above.
[0009] In one alternative approach, the exhaust channel extends in a serpentine, meandering pattern.
[0010] In one alternative embodiment, a sheet structure is formed on one side of the cartridge, and both the first reaction chamber and the second reaction chamber are disposed inside the sheet structure, wherein the wall thickness of the sheet structure is less than the wall thickness of the rest of the cartridge.
[0011] In one alternative embodiment, the cartridge includes a cartridge body and a film. The sample processing chamber, the first reaction chamber, the second reaction chamber, and the liquid injection channel are a plurality of grooves formed on the cartridge body. The film is attached to the cartridge body and closes the openings of the grooves.
[0012] In one alternative approach, the main channel is provided with a pressure relief chamber with an increased cross-sectional area, and the portion of the membrane that seals the pressure relief chamber is designed to deform under pressure from the sample liquid within the pressure relief chamber.
[0013] In one alternative configuration, the main channel extends horizontally, and the pressure relief chamber has a sedimentation tank formed by a downward indentation, which is used to deposit particulate matter in the flowing sample solution.
[0014] According to another aspect of the embodiments of this application, a nucleic acid detection system is provided, including a detection device and a nucleic acid detection cartridge as described above, wherein the detection device is used to fix and operate the nucleic acid detection cartridge to perform nucleic acid detection.
[0015] In the nucleic acid detection kit provided in this application embodiment, in addition to setting multiple reaction chambers (including a first reaction chamber and a second reaction chamber) to meet the testing needs of different items, a flow-blocking section is also set on the second branch channel used for injecting liquid into the second reaction chamber. This section is connected to the output end of the main channel and extends upward. A flow-blocking cavity with an increased cross-sectional area is set on the flow-blocking section. On the one hand, as the sample liquid enters the flow-blocking section and reaches the flow-blocking cavity, the height gradually increases and the flow resistance gradually increases. This causes the sample liquid to preferentially flow into the first reaction chamber through the first branch channel. After the first reaction chamber is filled, the sample liquid overcomes the resistance at the flow-blocking cavity and begins to flow into the second reaction chamber, thus filling the second reaction chamber. On the other hand, by utilizing the characteristic that air bubbles move upward due to buoyancy in the sample liquid, if air bubbles are mixed in the sample liquid during the injection of the sample liquid into the first reaction chamber, the air bubbles will move upward and enter the flow-blocking section. This ensures that there are as few air bubbles as possible in the sample liquid injected into the first reaction chamber. This ensures that the test liquid generated by the reaction between the sample liquid and the reaction reagent in the first reaction chamber can fill the internal space of the first reaction chamber, ensuring that the test results of the test liquid in the first reaction chamber are more accurate and reliable.
[0016] The above description is only an overview of the technical solution of this application. In order to better understand the technical means of this application and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this application more obvious and understandable, the following are specific embodiments of this application. Attached Figure Description
[0017] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:
[0018] Figure 1 A perspective view of one side of the nucleic acid detection card box provided in an embodiment of this utility model;
[0019] Figure 2 A perspective view of the nucleic acid testing card box from another side, provided in an embodiment of this utility model;
[0020] Figure 3 A perspective view of the nucleic acid detection card box provided in this embodiment of the utility model from the front view;
[0021] Figure 4 A bottom view of the nucleic acid testing card box provided in this embodiment of the utility model;
[0022] Figure 5 An exploded view of one side of the nucleic acid testing cartridge provided in an embodiment of this utility model;
[0023] Figure 6 An exploded view of the nucleic acid test kit provided in this embodiment of the present invention from another side.
[0024] The reference numerals in the detailed embodiments are as follows:
[0025] 500, card box;
[0026] 110. Sample processing chamber; 120. First reaction chamber; 130. Second reaction chamber;
[0027] 140. Injection channel; 141. Main channel; 1411. Pressure relief chamber; 1412. Sedimentation tank; 142. First branch channel; 143. Second branch channel; 1431. Flow-blocking section; 1432. Flow-blocking chamber;
[0028] 150. Vent; 160. Waste liquid chamber; 170. Waterproof and breathable membrane;
[0029] 181. First flow channel; 182. Second flow channel; 183. Third flow channel; 184. Exhaust flow channel;
[0030] 190. Shut-off valve; 200. Thin sheet structure; 210. Card holder body; 220. Membrane. Detailed Implementation
[0031] The embodiments of the technical solution of this application will now be described in detail with reference to the accompanying drawings. These embodiments are only used to more clearly illustrate the technical solution of this application and are therefore merely examples, and should not be used to limit the scope of protection of this application.
[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms “comprising” and “having”, and any variations thereof, in the specification, claims, and foregoing description of the drawings are intended to cover non-exclusive inclusion.
[0033] In the description of the embodiments of this application, technical terms such as "first" and "second" are used only to distinguish different objects and should not be construed as indicating or implying relative importance or implicitly specifying the number, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, "multiple" means two or more, unless otherwise explicitly defined.
[0034] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.
[0035] In the description of the embodiments in this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent three cases: A exists, A and B exist simultaneously, and B exists. In addition, the character " / " in this document generally indicates that the related objects before and after it have an "or" relationship.
[0036] In the description of the embodiments of this application, the term "multiple" refers to two or more (including two), similarly, "multiple sets" refers to two or more (including two sets), and "multiple pieces" refers to two or more (including two pieces).
[0037] In the description of the embodiments of this application, the technical terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of this application and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.
[0038] In the description of the embodiments of this application, unless otherwise expressly specified and limited, technical terms such as "installation," "connection," "joining," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. For those skilled in the art, the specific meaning of the above terms in the embodiments of this application can be understood according to the specific circumstances.
[0039] In order to meet the testing needs of different items for sample solutions, this application first adopts the method of setting up multiple reaction chambers, with different reaction chambers performing different testing items respectively.
[0040] Based on this, practical verification revealed that if multiple reaction chambers are injected with sample liquid simultaneously, air bubbles trapped within them are obstructed by the preceding and following sample liquids. After injection into the reaction chamber, these bubbles are difficult to expel, resulting in the presence of air bubbles that cannot be fully filled by the detection liquid. Therefore, this application also designs the injection channels for different reaction chambers accordingly. Specifically, taking two reaction chambers (the first and second reaction chambers) as an example, an upwardly extending flow-blocking section is provided on the second branch channel connecting the main channel output end and the second reaction chamber. The flow-blocking section is connected to the main channel output end, and a flow-blocking cavity with an increased cross-sectional area is provided on the flow-blocking section. This causes the flow resistance of the sample liquid flowing into the flow-blocking cavity to gradually increase as the height increases. This results in the sample liquid preferentially flowing into the first branch channel connecting the main channel output end and the first reaction chamber. In the flow process, when the sample liquid flows from the output end of the main channel into the first branch channel, the air bubbles mixed in with the sample liquid will move upward. Since the flow-blocking section is connected to the output end of the main channel and extends upward, the air bubbles in the sample liquid will enter the flow-blocking section and move upward to the liquid surface in the flow-blocking cavity. This ensures that there are basically no air bubbles in the sample liquid injected into the first reaction cavity through the first branch channel. This ensures that after the sample liquid injected into the first reaction cavity reacts with the reaction reagent in the first reaction cavity, the resulting detection liquid can fill the first reaction cavity, thus ensuring the accuracy of subsequent detection results.
[0041] According to one aspect of the embodiments of this application, a nucleic acid detection kit is provided; please refer to [the specific details]. Figures 1 to 3 , Figure 1 and Figure 2 The image shows the three-dimensional structure of both sides of the card case. Figure 3 The image shows the perspective structure of the card box from a frontal view. It should be noted that... Figure 3 In the specific embodiment shown, each flow channel and cavity is formed by a groove on the card box, and the groove opening is covered with a film or baffle to seal the cavity and flow channel. The black solid line in the figure represents the groove structure that can be seen from the front, and the gray dashed line represents the groove structure that cannot be seen from the back. The slender extension in the figure is the flow channel. The flow channels are interconnected by through holes that penetrate the card box at the junction of the solid and dashed lines. It should be understood that the figure is only an example of a card box provided in this application. In other embodiments, all flow channels and cavities may be provided on one side of the card box, or an integrally molded card box may be used, and the cavity and flow channel are formed inside the card box during the injection molding process.
[0042] like Figure 3As shown, the cartridge 500 contains a sample processing chamber 110, a first reaction chamber 120, and a second reaction chamber 130. The sample processing chamber 110 is connected to both the first reaction chamber 120 and the second reaction chamber 130 via a liquid injection channel 140 on the cartridge 500. The liquid injection channel 140 includes a main channel 141, a first branch channel 142, and a second branch channel 143. The input end of the main channel 141 is connected to the sample processing chamber 110. The first branch channel 142 is connected between the output end of the main channel 141 and the first reaction chamber 120. The second branch channel 143 is connected between the output end of the main channel 141 and the second reaction chamber 130. The second branch channel 143 has a flow-blocking section 1431 that is connected to the output end of the main channel 141 and extends upward. A flow-blocking cavity 1432 with an increased cross-sectional area is provided on the flow-blocking section 1431. It should be noted here that "the upwardly extending flow-blocking section 1431" refers to the fact that, along the direction of sample injection, the flow-blocking section 1431 generally trendes upward from the input end to the output end. For example, it could be... Figure 3 The upward tilt shown can also be a vertical upward tilt, but this is not a specific limitation here.
[0043] In the specific testing process, the sample solution processed in the sample processing chamber 110 is generally a sample solution mixed with nucleic acid. As the sample solution is injected from the sample processing chamber 110 into the first reaction chamber 120 and the second reaction chamber 130 through the injection channel 140, it first flows through the main flow channel 141 and then enters the first branch flow channel 142 and the second branch flow channel 143 respectively. Gas in the sample solution preferentially flows upward into the flow-blocking section 1431, resulting in very few air bubbles in the sample solution flowing into the first branch flow channel 142. As the sample solution enters the flow-blocking section 1431 and reaches the flow-blocking chamber 1432, its height gradually increases, and the flow resistance gradually increases. Based on this, the sample solution preferentially flows towards the position with less resistance, that is, it preferentially flows into the first reaction chamber 120 through the first branch flow channel 142, thus filling the first reaction chamber 120 first. After the first reaction chamber 120 is filled, the sample solution overcomes the resistance at the flow-blocking chamber 1432 and flows into the second reaction chamber 130 to fill it.
[0044] Therefore, in the nucleic acid detection kit provided in this application embodiment, in addition to setting multiple reaction chambers (including a first reaction chamber 120 and a second reaction chamber 130) to meet the testing needs of different items, a flow-blocking section 1431 connected to the output end of the main flow channel 141 and extending upward is set on the second branch channel 143 used for injecting liquid into the second reaction chamber 130. A flow-blocking cavity 1432 with an increased cross-sectional area is set on the flow-blocking section 1431. On the one hand, as the sample liquid enters the flow-blocking section 1431 and reaches the flow-blocking cavity 1432, the height gradually increases and the flow resistance gradually increases, so that the sample liquid will preferentially flow into the first reaction chamber 120 through the first branch channel 142. After the first reaction chamber 120 is filled, the sample liquid overcomes the resistance at the flow-blocking chamber 1432 and begins to flow into the second reaction chamber 130, thus filling the second reaction chamber 130. On the other hand, by utilizing the characteristic that bubbles move upward due to buoyancy in the sample liquid, if there are bubbles in the sample liquid during the injection of the sample liquid into the first reaction chamber 120, the bubbles will move upward into the flow-blocking section 1431, thereby ensuring that there are as few bubbles as possible in the sample liquid injected into the first reaction chamber 120. This ensures that the test liquid generated by the reaction between the sample liquid and the reaction reagent in the first reaction chamber 120 can fill the internal space of the first reaction chamber 120, ensuring that the test results of the test liquid in the first reaction chamber 120 are more accurate and reliable.
[0045] Regarding the driving mechanism of the sample solution during injection, this application further proposes an implementation method, please refer to [link to details]. Figure 1 and Figure 3 As shown in the figure, the cartridge 500 is provided with an air hole 150 that communicates with the sample processing chamber 110. The air hole 150 communicates with the sample processing chamber 110 through an air channel provided inside the cartridge 500. During liquid injection, positive pressure is applied to the air hole 150 to drive the sample liquid in the sample processing chamber 110 to be injected into the first reaction chamber 120 and the second reaction chamber 130 through the liquid injection channel 140.
[0046] Specifically, when conducting nucleic acid testing, the cartridge 500 can be used in conjunction with testing equipment. The testing equipment is equipped with a gas pipeline for connecting to the vent 150. Gas is blown into the vent 150 through the gas pipeline to achieve positive pressure access to the vent 150.
[0047] In this embodiment, by providing an air hole 150 on the cartridge 500 that communicates with the sample processing chamber 110, and by using the air hole 150 to apply positive pressure to drive the sample liquid, the liquid injection process is made efficient and controllable.
[0048] In addition to using the positive pressure through the vent 150 to drive the liquid injection, the first reaction chamber 120 and the second reaction chamber 130 can be evacuated during the production of the cartridge 500, and the sample liquid can be injected into the first reaction chamber 120 and the second reaction chamber 130 by utilizing the pressure difference between the sample processing chamber 110 and the first reaction chamber 120 and the second reaction chamber 130.
[0049] During the injection process, if gas is present in the sample solution, in order to smoothly expel the gas and ensure that the first reaction chamber 120 and the second reaction chamber 130 are better filled with the sample solution, venting can also be performed at the tail end of the first reaction chamber 120 and the second reaction chamber 130. For details, please refer to [link to relevant documentation]. Figure 2 and Figure 3 As shown in the figure, the cartridge 500 is also equipped with a waste liquid chamber 160. The input end of the first reaction chamber 120 is connected to the first branch channel 142, and the output end is connected to the waste liquid chamber 160 through a channel. Similarly, the input end of the second reaction chamber 130 is connected to the second branch channel 143, and the output end is connected to the waste liquid chamber 160 through a channel. Furthermore, a water-proof and gas-permeable membrane 170 is provided in the channel between the first reaction chamber 120, the second reaction chamber 130, and the waste liquid chamber 160. The water-proof and gas-permeable membrane 170 is used to block the sample liquid and allow the gas in the sample liquid to be discharged into the waste liquid chamber 160. Specifically, the gas present in the sample liquid located between the liquid inlet channel of the first reaction chamber 120 and the second reaction chamber 130 and the water-proof and gas-permeable membrane 170 can be discharged into the waste liquid chamber 160 through the water-proof and gas-permeable membrane 170 to ensure that there is no gas in the first reaction chamber 120 and the second reaction chamber 130, thus ensuring the accuracy of the detection.
[0050] exist Figure 3 In the specific embodiment shown, to simplify the structure, the first reaction chamber 120, the second reaction chamber 130, and the waste liquid chamber 160 share the same water-proof and breathable membrane 170. Specifically, the cartridge 500 has three closely spaced through holes. The output end of the first reaction chamber 120 is connected to one of these through holes via a first flow channel 181, the output end of the second reaction chamber 130 is connected to another through hole via a second flow channel 182, and the last through hole is connected to the waste liquid chamber 160 via a third flow channel 183. The water-proof and breathable membrane 170 covers all three through holes, allowing gas arriving at the water-proof and breathable membrane 170 from the first flow channel 181 and the second flow channel 182 to pass through the membrane and then be discharged into the waste liquid chamber 160 via the third flow channel 183 during the injection process. This ensures the stability of the pressure in the first reaction chamber 120 and the second reaction chamber 130 during the injection process. The sample liquid reaching the water-proof and breathable membrane 170 from the first flow channel 181 and the second flow channel 182 will be blocked by the water-proof and breathable membrane 170 to ensure that the sample liquid can be filled in the first reaction chamber 120 and the second reaction chamber 130.
[0051] In another embodiment, the three through holes may be omitted, and the water-proof and breathable membrane 170 may be directly disposed in the third flow channel 183, or the water-proof and breathable membrane 170 may be disposed in the first flow channel 181 and the second flow channel 182 respectively. Of course, the third flow channel 183 may also be omitted, and the output end of the first reaction chamber 120 may be directly connected to the waste liquid chamber 160 through the first flow channel 181, and the output end of the second reaction chamber 130 may be directly connected to the waste liquid chamber 160 through the second flow channel 182, with the water-proof and breathable membrane 170 disposed in the first flow channel 181 and the second flow channel 182 respectively.
[0052] As mentioned in the above embodiments, during the injection process, the sample solution is preferentially injected into the first reaction chamber 120, and the gas in the sample solution will first move upward to the liquid surface above the flow barrier 1432. On this basis, after the first reaction chamber 120 is filled, the sample solution begins to flow into the second reaction chamber 130. At this time, the gas above the liquid surface of the flow barrier 1432 will be pushed by the sample solution and pass through the second reaction chamber 130, the second flow channel 182, and the water-proof and breathable membrane 170 in sequence before entering the third flow channel 183. This allows the second reaction chamber 130 to be better filled with sample solution, thus ensuring the accuracy of the detection results.
[0053] In addition, in order to improve the accuracy of detection, such as Figure 3 As shown, a shut-off valve 190 can be installed on the first branch channel 142, the second branch channel 143, the first channel 181, and the second channel 182. The shut-off valve 190 is configured to close after liquid injection is completed in the first reaction chamber 120 and the second reaction chamber 130.
[0054] Specifically, the shut-off valve 190 can be adopted Figure 3 The membrane valve shown specifically has a through-hole at the blocking valve 190 in the cartridge 500. The corresponding flow channels are located at both ends on both sides of the cartridge 500, and the flow channels on both sides are connected through this through-hole. The blocking valve 190 is formed by a membrane located at the through-hole. After liquid injection, the through-hole on the corresponding flow channel is sealed by pressing the membrane, thus isolating the flow channels on both sides from each other. After liquid injection into the first reaction chamber 120 and the second reaction chamber 130 is completed, the blocking valve 190 closes, sealing the first reaction chamber 120 and the second reaction chamber 130. This ensures that the first reaction chamber 120 and the second reaction chamber 130 maintain a certain volume for thermal cycling and detection during subsequent thermal cycling, thus ensuring the accuracy of the detection results.
[0055] In order to better expel the gas mixed in the sample solution from the first reaction chamber 120 and the second reaction chamber 130, such as Figure 1As shown, the flow channel between the output end of the first reaction chamber 120 and the water-proof and breathable membrane 170 (i.e., the first flow channel 181) and the flow channel between the output end of the second reaction chamber 130 and the water-proof and breathable membrane 170 (i.e., the second flow channel 182) are both connected to an exhaust flow channel 184 above. As mentioned above, air bubbles mixed in the sample liquid will move upwards under the action of buoyancy. Based on this, in this embodiment, by connecting the exhaust flow channel 184 above the first flow channel 181 and the second flow channel 182, air bubbles mixed in the sample liquid can move upwards and enter the exhaust flow channel 184, thereby ensuring that there is as little gas as possible in the first reaction chamber 120 and the second reaction chamber 130.
[0056] In addition, the exhaust channel 184 has no output end. During the process of injecting liquid into the first reaction chamber 120 and the second reaction chamber 130, as the gas enters the exhaust channel and the liquid level rises during the injection process, the gas in the exhaust channel 184 will be compressed, causing the pressure inside the exhaust channel 184 to gradually increase. Based on the gradual increase in pressure inside the exhaust channel 184, the sample liquid cannot enter the exhaust channel 184 in large quantities.
[0057] Specifically, such as Figure 3 As shown, the exhaust channel 184 can extend in a serpentine shape. This serpentine extension can provide more space for gas to enter while occupying a smaller size, thus achieving better exhaust performance.
[0058] To improve detection efficiency, this application further proposes an implementation method, which can be found in the following details. Figure 2 The figure shows the bottom view of the card holder 500. As shown in the figure, a thin sheet structure 200 is formed on one side of the card holder 500. The first reaction chamber 120 is disposed inside the thin sheet structure 200, and the wall thickness of the thin sheet structure 200 is less than the wall thickness of the rest of the card holder 500. The second reaction chamber 130 is similar, and will not be described in detail here.
[0059] In this embodiment, by placing the first reaction chamber 120 and the second reaction chamber 130 within a thin sheet structure 200 with a thinner wall thickness, the heat loss when passing through the walls of the first reaction chamber 120 and the second reaction chamber 130 can be reduced during PCR detection of the detection solution in the first reaction chamber 120 and the second reaction chamber 130, thereby improving thermal conductivity, shortening amplification time, and ultimately achieving the goal of improving detection efficiency.
[0060] Considering the complexity and high cost of the one-piece molding of the microchannels inside the card holder 500, this application proposes a manufacturing method that facilitates mass production of the card holder 500 and effectively reduces costs. Please refer to [link / reference] for details. Figure 3 and Figure 3The exploded structure of the card holder 500 is shown from both sides in the figure. As shown in the figure, the card holder 500 includes a card holder body 210 and a film 220. The sample processing chamber 110, the first reaction chamber 120, the second reaction chamber 130 and the liquid injection channel 140 are several grooves opened on the card holder body 210. The film 220 is attached to the card holder body 210 and closes the groove openings.
[0061] In the specific production process, in order to ensure structural strength, the card box body 210 can be made of rigid material, while in order to ensure sealing performance, the film 220 can be made of flexible material. The two can be assembled and fixed by means of bonding, heat fusion and other methods.
[0062] Compared to the method of integrally molding the flow channel and cavity inside, the method provided in this embodiment of opening a groove in the card box body 210 and sealing the groove with a film 220 is more convenient for the production and manufacturing of the card box 500, which is conducive to improving production efficiency and reducing production costs.
[0063] Considering the high pressure driving the sample fluid flow during the injection process, and the relatively thin wall thickness at the first reaction chamber 120 and the second reaction chamber 130, to prevent excessive hydraulic pressure from damaging the first reaction chamber 120 and the second reaction chamber 130, such as... Figure 3 and Figure 3 As shown, a pressure relief chamber 1411 with an increased cross-sectional area can be provided on the main flow channel 141. During liquid injection, the pressure relief chamber 1411 will contain more sample liquid. Therefore, the hydraulic pressure in the pressure relief chamber 1411 is higher than that in other locations in the liquid injection channel 140. Based on this, the membrane 220 that seals the pressure relief chamber 1411 can release a portion of the pressure relief chamber 1411 through deformation. This ensures that the pressure in the liquid injection channel 140 is stable and prevents the pressure from concentrating in the first reaction chamber 120 and the second reaction chamber 130, which would damage the structure of the first reaction chamber 120 and the second reaction chamber 130.
[0064] The sample solution obtained in the sample processing chamber 110 is generally a liquid mixed with nucleic acid and magnetic beads. During the injection, the magnetic beads (i.e., lyophilized beads) are first adsorbed, and then the liquid mixed with nucleic acid is injected into the first reaction chamber 120 and the second reaction chamber 130. However, since there are a large number of magnetic beads, when some magnetic beads are not completely adsorbed, they may be mixed in with the liquid and injected into the first reaction chamber 120 and the second reaction chamber 130 together, which will affect the detection results.
[0065] To prevent any unadsorbed magnetic beads from entering the first reaction chamber 120 and the second reaction chamber 130, such as... Figure 3 and Figure 5 Figure 5 Figure 6 Figure 3 Figure 6 Figure 3 Figure 6As shown, the main channel 141 extends horizontally to allow the sample liquid to flow horizontally within it. The pressure relief chamber 1411 has a downwardly recessed sedimentation tank 1412, which is used to deposit particulate matter in the flowing sample liquid. Specifically, when the magnetic beads mixed in the sample liquid move horizontally to the sedimentation tank 1412 within the main channel 141, they sink to the bottom of the sedimentation tank 1412 under their own gravity. The sample liquid then flows through the sedimentation tank 1412 and enters the first reaction chamber 120 and the second reaction chamber 130. This filters out unadsorbed magnetic beads during the injection process, preventing them from entering the first reaction chamber 120 and the second reaction chamber 130 and affecting the detection results.
[0066] According to another aspect of the embodiments of this application, a nucleic acid detection system is also provided, the system including a detection device (not shown) and a nucleic acid detection cartridge 500 as described in any of the above embodiments, the detection device being used to fix and operate the nucleic acid detection cartridge for nucleic acid detection.
[0067] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and not to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application. In particular, as long as there is no structural conflict, the various technical features mentioned in the various embodiments can be combined in any way.
Claims
1. A nucleic acid testing cartridge, characterized by, The cartridge is provided with a sample processing cavity, a first reaction cavity and a second reaction cavity, the sample processing cavity is communicated with the first reaction cavity and the second reaction cavity through liquid injection channels on the cartridge respectively; The liquid injection channels comprise a main channel, a first branch channel and a second branch channel, the input end of the main channel is communicated with the sample processing cavity, the first branch channel is communicated between the output end of the main channel and the first reaction cavity, and the second branch channel is communicated between the output end of the main channel and the second reaction cavity; The second branch channel has a resistance flow section communicated with the output end of the main channel and extending upward, and the resistance flow section is provided with a resistance flow cavity with an increased cross-sectional area.
2. The nucleic acid testing cartridge of claim 1, wherein, The cartridge is provided with an air hole communicated with the sample processing cavity, the air hole is used to drive sample liquid in the sample processing cavity to be injected into the first reaction cavity and the second reaction cavity through the liquid injection channels when a positive pressure is applied.
3. The nucleic acid testing cartridge of claim 1, wherein, The cartridge is further provided with a waste liquid cavity, the input end of the first reaction cavity is communicated with the first branch channel, the output end of the first reaction cavity is communicated with the waste liquid cavity through a channel, the input end of the second reaction cavity is communicated with the second branch channel, and the output end of the second reaction cavity is communicated with the waste liquid cavity through a channel; A water-proof air-permeable membrane is arranged in the channel between the output end of the first reaction cavity, the output end of the second reaction cavity and the waste liquid cavity, the water-proof air-permeable membrane is used to block the sample liquid and allow the gas in the sample liquid to be discharged into the waste liquid cavity.
4. The nucleic acid testing cartridge of claim 3, wherein, The channel between the output end of the first reaction cavity and the water-proof air-permeable membrane and the channel between the output end of the second reaction cavity and the water-proof air-permeable membrane are both communicated with an exhaust channel upward.
5. The nucleic acid testing cartridge of claim 4, wherein, The exhaust channel extends in a serpentine shape.
6. The nucleic acid testing cartridge of claim 1, wherein, One side of the cartridge is formed with a sheet structure, the first reaction cavity and the second reaction cavity are arranged inside the sheet structure, and the wall thickness of the sheet structure is smaller than that of the rest of the cartridge.
7. The nucleic acid testing cartridge of claim 6, wherein, The cartridge comprises a cartridge main body and a film, the sample processing cavity, the first reaction cavity, the second reaction cavity and the liquid injection channels are a plurality of grooves formed on the cartridge main body, and the film is attached to the cartridge main body and closes the groove openings.
8. The nucleic acid testing cartridge of claim 7, wherein, The main channel is provided with a pressure relief cavity with an increased cross-sectional area, and the part of the film closing the pressure relief cavity is used to deform when subjected to the pressure of the sample liquid in the pressure relief cavity.
9. The nucleic acid testing cartridge of claim 8, wherein, The main channel extends in a horizontal direction, the pressure relief cavity has a sedimentation pool formed by being concave downward, and the sedimentation pool is used to deposit particulate matters in the sample liquid flowing therethrough.
10. A nucleic acid detection system, characterized by, The nucleic acid detection cartridge comprises a detection device and a nucleic acid detection cartridge according to any one of claims 1-9, and the detection device is used to fix and operate the nucleic acid detection cartridge to perform nucleic acid detection.