Automatic sample adding and detecting device for biological chip

Abstract

An automatic sample adding and detecting device for a biological chip relates to the technical field of biological chip detection and comprises a platform, a coordinate positioning mechanism, a supporting plate, a biological chip, a sealing plate, a detection hole, an automatic buffer solution feeder and a biological sample feeder, and the automatic buffer solution feeder and the biological sample feeder are identical in structure. Each feeder comprises a feeder body and a driving mechanism used for controlling the feeder body to quantitatively discharge liquid, a support is further fixedly arranged at the top end of the platform, an optical fiber probe is arranged on the support, and a shooting camera is arranged on the support on one side of the optical fiber probe. The liquid adding device does not need frequent sampling, can ensure that the liquid adding amount each time is consistent, avoids the tedious structure and operation of repeated sampling, promotes the detection result to be more accurate due to the uniform liquid adding amount, and is simple in structure, convenient to use, low in manufacturing cost, convenient to maintain and suitable for popularization and use.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biochip detection technology, and particularly relates to a biochip automatic sample adding and detecting device. BACKGROUND

[0002] A biochip detection platform is a system that uses biochips (also known as bioarrays) for high-throughput detection of biological molecules. A biochip is a technology that immobilizes a large number of biological probes, such as DNA, RNA, proteins, cells, etc., on the surface of a small carrier (usually glass or silicon wafer). Using a biochip detection platform, researchers can simultaneously screen and analyze thousands of biomarkers.

[0003] The main components and workflow of a biochip detection platform are as follows: Biochip design and fabrication: First, design the biological probes contained in the chip, then use machines to accurately print or deposit the probes on specific locations on the chip surface. Sample preparation: After processing, the biological samples to be tested, such as patient blood or tissue samples, are prepared for detection. Hybridization / binding: After processing, the sample is contacted with the biochip, so that the target molecules (such as specific DNA fragments, RNA or proteins) in the sample bind or hybridize with the corresponding probes on the chip. Signal detection and reading: The bound biological molecules are usually labeled by fluorescence or other means, so that they can be read by detection equipment (such as a fluorescence scanner). Data analysis: The acquired signal data is processed and interpreted by specialized analysis software to obtain information about the expression level of the target molecules in the sample.

[0004] The prior art CN118393159A discloses a biological sample detection platform based on a biosensor, which comprises a workbench, a mechanical arm one and a mechanical arm two arranged on the workbench, a detection support arranged on the workbench, a fiber probe arranged on the detection support, a shooting camera arranged on the detection support, a microtube array arranged on the workbench, a buffer array arranged on the workbench, and a sample array arranged on the workbench, and further comprises a detection unit arranged on the mechanical arm two. The biological sample detection platform based on the biosensor is provided with a petal-shaped groove at the opening above the micro-well. After the sample is dropped into the micro-well, the mechanical arm two is used to gently shake the sample to make it turn in the petal-shaped groove, thereby increasing the contact probability between the sample and the biochip, accelerating the reaction speed and improving the signal-to-noise ratio of the chip detection.

[0005] The above patent document has the following defects:

[0006] (1) In the detection work, each type of sample needs to be detected multiple times, so repeated sampling is required, which is difficult to ensure the consistency of the sampling amount each time, not only the operation is complicated, but also it is easy to cause detection errors.

[0007] (2) The operation is carried out using complex robotic arm components, which results in high manufacturing costs, complex control systems, and inconvenient maintenance. Utility Model Content

[0008] This invention provides an automated sample addition and detection device for biochips, which aims to solve the problems described in (1) and (2) of the prior art.

[0009] To achieve the above objectives, the new technical solution is as follows:

[0010] An automated sample dispensing and detection device for a biochip includes a platform, a coordinate positioning mechanism at the top of the platform, a tray connected to the top of the coordinate positioning mechanism, a biochip at the top of the tray, and a sealing plate connected to the top of the biochip. The sealing plate has a plurality of detection holes arranged in an array and communicating with the biochip through the detection holes. The detection holes are sealed to each other. An automatic buffer feeder and a biological sample feeder are also arranged side by side at the top of the platform. The automatic buffer feeder and the biological sample feeder have the same structure, each including a feeder body and a drive mechanism for controlling the quantitative dispensing of liquid from the feeder body. A bracket is also fixed at the top of the platform, and an optical fiber probe is mounted on the bracket. A camera is mounted on the bracket on one side of the optical fiber probe.

[0011] Preferably, a portal frame is fixedly connected to the top of the platform, and the two free ends of the portal frame are fixedly connected to the middle of the two ends of the platform respectively. An automatic buffer feeder and a biological sample feeder are fixedly installed on one side of the lower surface of the top of the portal frame, and a support is installed on the other side. An optical fiber probe is fixedly connected to the outer wall of the support through a connector. The signal line of the optical fiber probe passes through the top of the portal frame and is connected to a spectrometer.

[0012] Preferably, the coordinate positioning mechanism includes first lead screws distributed at the front and rear ends of the platform top. The first lead screws are arranged in the left-right direction, and mounting plates are rotatably connected to both ends of the first lead screws. The mounting plates are fixedly connected to the top of the platform. A first servo motor is fixedly mounted on the outer side of one mounting plate, and two first servo motors are synchronously driven under the control of the detection device's control system. A movable support plate is screwed onto both first lead screws. The front and rear ends of the movable support plate are respectively screwed to the corresponding first lead screws. A fixed plate is fixedly connected to the top of the movable support plate. The fixed plate has a strip groove in the front-back direction. A second lead screw is arranged in the strip groove in the front-back direction. The two ends of the second lead screw are rotatably connected to the two ends of the strip groove. A second servo motor is also fixedly mounted on one end of the fixed plate. The output shaft of the second servo motor is fixedly connected to the end of the second lead screw and is used to drive the second lead screw to rotate. A movable seat is slidably connected in the strip groove. The movable seat is screwed to the second lead screw, and a support plate is fixedly mounted on the top of the movable seat. The second servo motor is electrically connected to the control system through wires.

[0013] Preferably, the feeder body includes a liquid storage bottle located at the top, a guide tube coaxially reduced in diameter connected to the bottom end of the liquid storage bottle, and a sealing tube coaxially reduced in diameter connected to the bottom end of the guide tube. A guide block is slidably connected inside the guide tube. A quantitative liquid dispensing rod is coaxially fixedly connected to the bottom end of the guide block. The quantitative liquid dispensing rod is slidably and sealingly connected to the sealing tube. A top rod is coaxially fixedly connected to the top end of the guide block. The top rod passes through a sliding hole at the top of the liquid storage bottle and is fixedly connected to a connecting plate.

[0014] Preferably, the outer wall surface of the quantitative liquid dispensing rod is evenly distributed with several liquid storage tanks along the axial direction, the top of the side wall of the liquid storage bottle is provided with a liquid addition pipe, the liquid addition pipe is provided with a sealing plug, and the guide block is provided with a through flow hole.

[0015] Preferably, the connecting plate is fixedly connected to the lower surface of the top of the gantry frame via a connecting rod, and the driving mechanism is an electrically controlled telescopic mechanism arranged longitudinally, which is connected between the lower surface of the top plate of the gantry frame and the top of the outer wall of the liquid storage bottle.

[0016] Preferably, the electrically controlled telescopic mechanism is an electric cylinder, and there are two electric cylinders symmetrically arranged on both sides of the connecting rod. The fixed end of the electric cylinder is fixedly connected to the lower surface of the top of the gantry frame, and the telescopic end is fixedly connected to the top of the outer wall of the liquid storage bottle. The connecting plate is detachably fixedly connected to the connecting rod, and the telescopic end of the electric cylinder is detachably fixedly connected to the outer wall of the liquid storage bottle.

[0017] Preferably, the upper part of the detection hole is enlarged to form a funnel-shaped splash-proof groove.

[0018] The beneficial effects of this novel automated biochip sampling and detection device are as follows:

[0019] This new type of device eliminates the need for frequent sampling, ensuring consistent liquid volume each time. It avoids the cumbersome structure and operation of multiple sampling, and the uniform liquid volume promotes more accurate test results. This new device has a simple structure, is easy to use, has low manufacturing cost, is easy to maintain, and is suitable for widespread use. Attached Figure Description

[0020] Figure 1 A schematic diagram of the front view of this novel structure;

[0021] Figure 2 A top view of the structure of this new platform;

[0022] Figure 3 A top view of the structure of this novel platform after it is connected to a tray, a biochip, and sealed.

[0023] Figure 4 A top view of the new platform with a portal frame connected to the top;

[0024] Figure 5 A schematic diagram of the structure of the feeder body of this novel device.

[0025] The above figures are used to explain the working principle of this invention and are not intended to limit the specific structure and dimensions of this invention.

[0026] In the diagram: 1. Platform; 2. First lead screw; 3. Mounting plate; 4. First servo motor; 5. Fixing plate; 6. Second servo motor; 7. Support plate; 8. Biochip; 9. Detection port; 10. Support; 11. Fiber optic probe; 12. Camera; 13. Automatic buffer feeder; 14. Biological sample feeder; 15. Feeder body; 151. Storage bottle; 152. Guide tube; 153. Guide block; 154. Flow hole; 155. Quantitative dispensing rod; 156. Storage tank; 157. Sealing tube; 158. Top rod; 159. Connecting plate; 1510. Dispensing tube; 1511. Sealing plug; 16. Moving support plate; 17. Second lead screw; 18. Moving seat; 19. Sealing plate; 20. Gantry frame; 21. Signal line; 22. Connecting rod; 23. Electric cylinder. Detailed Implementation

[0027] The following is a detailed description of the embodiments of the present invention in a step-by-step manner. This description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

[0028] In the description of this invention, it should be noted that the terms "upper," "lower," "left," "right," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or a specific orientational structure and operation. Therefore, they should not be construed as limitations on this invention.

[0029] The following embodiments can be understood as part of the technical structure and principle of this invention, or as a combination of multiple embodiments explaining a wider range of structures and principles of this invention.

[0030] Example 1

[0031] This novel automated sample dispensing and detection device for biochips, such as... Figures 1-5 As shown, the system includes a platform 1, a coordinate positioning mechanism at the top of the platform 1, a support plate 7 connected to the top of the coordinate positioning mechanism, a biochip 8 at the top of the support plate 7, and a sealing plate 19 connected to the top of the biochip 8. The sealing plate 19 has several arrayed detection holes 9, which communicate with the biochip 8. The detection holes 9 are sealed to each other to prevent liquid contamination. The top of the platform 1 also has an automatic buffer feeder 13 and a biological sample feeder 14 arranged side by side. The automatic buffer feeder 13 and the biological sample feeder 14 have the same structure, both including a feeder body 15 and a drive mechanism for controlling the quantitative liquid dispensing of the feeder body 15. A bracket 10 is also fixed at the top of the platform 1. The bracket 10 has an optical fiber probe 11, and a camera 12 is mounted on the bracket 10 on one side of the optical fiber probe 11.

[0032] In this embodiment, the coordinate positioning mechanism is used to move and position each detection well, so that the detection well is aligned with the positions of the automatic buffer feeder, the biological sample feeder, and the fiber optic probe, thereby achieving automated detection under the control of the control system. The feeder body 15 is used to store buffer solution or biological samples and also has the function of multiple quantitative dispensing, avoiding the cumbersome operation caused by frequent dispensing and ensuring that the dispensing volume is consistent each time.

[0033] Example 2

[0034] like Figure 1 , 4 As shown, a portal frame 20 is fixedly connected to the top of the platform 1. The two free ends of the portal frame 20 are fixedly connected to the middle of both ends of the platform 1. An automatic buffer feeder 13 and a biological sample feeder 14 are fixedly installed on one side of the lower top surface of the portal frame 20, and a support 10 is installed on the other side. An optical fiber probe 11 is fixedly connected to the outer wall of the support 10 through a connector. The signal line of the optical fiber probe 11 passes through the top of the portal frame 20 and is connected to a spectrometer.

[0035] Example 3

[0036] like Figures 1-4 As shown, the coordinate positioning mechanism includes first lead screws 2 distributed at the front and rear ends of the top of platform 1. The first lead screws 2 are arranged in the left-right direction, and mounting plates 3 are rotatably connected to both ends of the first lead screws 2. The mounting plates 3 are fixedly connected to the top of platform 1. A first servo motor 4 is fixedly mounted on the outside of one mounting plate 3. The two first servo motors 4 are synchronously driven under the control of the control system of the detection device (or synchronously drive the two first lead screws through sprocket and chain transmission). A movable support plate 16 is screwed onto both first lead screws 2. The front and rear ends of the movable support plate 16 are respectively screwed to the corresponding first lead screw 2. A fixed plate 5 is fixedly connected to the top of the movable support plate 16. The fixed plate 5 has a strip groove (such as...) opened in the front-back direction. Figure 2 As shown in the figure (not marked), a second lead screw 17 is provided in the strip groove along the front-back direction. The two ends of the second lead screw 17 are rotatably connected to the two ends of the strip groove. A second servo motor 6 is also fixedly provided at one end of the fixed plate 5. The output shaft of the second servo motor 6 is fixedly connected to the end of the second lead screw 17 and is used to drive the second lead screw 17 to rotate. A movable seat 18 is slidably connected in the strip groove. The movable seat 18 is screwed to the second lead screw 17. A support plate 7 is fixedly provided at the top of the movable seat 18. The second servo motor 6 is electrically connected to the control system through wires.

[0037] In this embodiment, the principle of coordinate control is as follows: the rotation of the first servo motor precisely controls the X-axis coordinate of each detection hole 9, and the rotation of the second servo motor further precisely controls the Y-axis coordinate of each detection hole 9. Through coordinate control, the positions of the detection holes relative to the buffer automatic feeder, biological sample feeder, and fiber optic probe can be made relative, thereby realizing the automated control of the displacement and positioning of each process of buffer feeding, biological sample feeding, and biological detection.

[0038] Example 4

[0039] like Figure 1 , 5 As shown, the feeder body 15 includes a liquid storage bottle 151 located at the top, a guide tube 152 coaxially reduced in diameter connected to the bottom end of the liquid storage bottle 151, and a sealing tube 157 coaxially reduced in diameter connected to the bottom end of the guide tube 152. A guide block 153 is slidably connected inside the guide tube 152. A quantitative liquid dispensing rod 155 is coaxially fixedly connected to the bottom end of the guide block 153. The quantitative liquid dispensing rod 155 is slidably and sealed to the sealing tube 157. A top rod 158 is coaxially fixedly connected to the top end of the guide block 153. The top rod 158 passes through a sliding hole at the top of the liquid storage bottle 151 and is fixedly connected to a connecting plate 159.

[0040] like Figure 1 , 5 As shown, the outer wall surface of the quantitative liquid dispensing rod 155 is evenly distributed with several liquid storage tanks 156 along the axial direction. The top of the side wall of the liquid storage bottle 151 is provided with a liquid addition pipe 1510. The liquid addition pipe 1510 is provided with a sealing plug 1511. The guide block 153 is provided with a through flow hole 154.

[0041] like Figure 1 , 5 As shown, the connecting plate 159 is fixedly connected to the lower surface of the top of the gantry frame 20 via the connecting rod 22. The driving mechanism is an electrically controlled telescopic mechanism arranged in the longitudinal direction. The electrically controlled telescopic mechanism is connected between the lower surface of the top plate of the gantry frame 20 and the top of the outer wall of the liquid storage bottle 151.

[0042] like Figure 1 , 5 As shown, the electrically controlled telescopic mechanism is an electric cylinder 23. There are two electric cylinders 23, which are symmetrically arranged on both sides of the connecting rod 22. The fixed end of the electric cylinder 23 is fixedly connected to the lower top surface of the gantry frame 20, and the telescopic end is fixedly connected to the top of the outer wall of the liquid storage bottle 151. The connecting plate is detachably fixedly connected to the connecting rod, and the telescopic end of the electric cylinder is detachably fixedly connected to the outer wall of the liquid storage bottle.

[0043] In this embodiment, the principle of quantitative sample addition is as follows: when a detection well moves to below the automatic buffer feeder 13 and the biological sample feeder 14, the electric cylinder retracts, pulling the storage bottle upwards by a set distance. At this time, one or more storage tanks pass through the sealing tube and are exposed. Due to the loss of the constraint of the sealing tube, the liquid in the storage tank slides down along the quantitative sampling rod into the detection well 9, realizing quantitative liquid addition. When the quantitative sampling rod retracts into the guide tube, liquid re-enters the storage tank. This process is repeated to avoid frequent sampling actions and ensure that quantitative liquid addition is performed each time.

[0044] Example 5

[0045] like Figure 1 , 3 As shown, the upper part of the detection hole 9 forms a funnel-shaped anti-splash groove by expanding its diameter. Normally, the buffer solution needs to be thoroughly mixed with the biological sample to ensure the accuracy of the detection results. In this novel design, a coordinate positioning mechanism moves the detection hole repeatedly back and forth and left and right until a mixing effect is achieved. The anti-splash groove is to prevent liquid from splashing out of the detection hole; the amount of liquid added to the detection hole should be controlled to the portion below the anti-splash groove.

[0046] In use, sufficient buffer solution and biological sample are added to the automatic buffer solution feeder 13 and the biological sample feeder 14, respectively. When the detection well moves under the drive of the coordinate positioning mechanism to below the automatic buffer solution feeder 13 and the biological sample feeder 14, quantitative amounts of buffer solution and biological sample are added sequentially. Then, the detection well moves under the fiber optic probe for biochip detection. During the above process, buffer solution and biological sample can be added to several detection wells in batches, and then the wells can be moved under the fiber optic probe for batch biological detection.

[0047] It should be noted that this invention is an improvement based on existing technology, and other unmentioned aspects are addressed by existing solutions.

Claims

1. An automated sample dispensing and detection device for biochips, characterized in that: The platform is provided with a coordinate positioning mechanism at the top end, a supporting plate connected to the top of the coordinate positioning mechanism, a biochip provided at the top end of the supporting plate, and a sealing plate connected to the top end of the biochip, wherein the sealing plate is provided with a plurality of detection holes arranged in an array and in communication with the biochip through the detection holes, and the detection holes are sealed from each other.

2. The automatic biochip sample adding and detecting device according to claim 1, characterized in that: The top of the platform is further provided with a bracket, and the bracket is provided with a fiber probe and a shooting camera.

3. The biochip automated sample dispensing and detection device as described in claim 2, characterized in that: The coordinate positioning mechanism comprises first lead screws distributed at the front and rear ends of the top of the platform, the first lead screws are arranged in the left-right direction, and the two ends of each first lead screw are rotatably connected to a mounting plate fixedly connected to the top of the platform.

4. The automatic biochip sample adding and detecting device according to claim 3, characterized in that: The first servo motors are fixedly arranged outside one of the mounting plates and are synchronously driven under the control of the control system of the detection device.

5. The automatic biochip sample adding and detecting device according to claim 4, characterized in that: The first lead screws are screw-connected to a moving supporting plate, the moving supporting plate is screw-connected to the front and rear ends of the corresponding first lead screws, and the top of the moving supporting plate is fixedly connected to a fixed plate. The fixed plate is provided with a strip-shaped groove in the front-rear direction, and a second lead screw is arranged in the strip-shaped groove in the front-rear direction. The two ends of the second lead screw are rotatably connected to the two ends of the strip-shaped groove, one end of the fixed plate is further fixedly provided with a second servo motor, the output shaft of the second servo motor is fixedly connected to the end of the second lead screw, and the second servo motor is used to drive the second lead screw to rotate. The moving seat is screw-connected to the second lead screw, and the top end of the moving seat is fixedly provided with a supporting plate. The second servo motor is electrically connected to the control system through wires. The liquid taking rod is sealingly and slidingly connected to the sealing tube, the top end of the guide block is fixedly connected to a jack, and the jack penetrates the sliding hole in the top of the liquid storage bottle and is fixedly connected to a connecting plate. The outer wall surface of the liquid taking rod is uniformly distributed with a plurality of liquid storage grooves in the axial direction. The side wall of the liquid storage bottle is provided with a liquid adding pipe at the top, the liquid adding pipe is provided with a sealing plug, and the guide block is provided with a through flow hole.

6. The automatic biochip sample adding and detecting device according to claim 5, characterized in that: The connecting plate is fixedly connected with the lower surface of the top of the door-shaped frame through a connecting rod, the driving mechanism is an electric control telescopic mechanism arranged along the longitudinal direction, and the electric control telescopic mechanism is connected between the lower surface of the top plate of the door-shaped frame and the top end of the outer wall of the liquid storage bottle.

7. The automatic biochip sample adding and detecting device according to claim 6, characterized in that: The electric control telescopic mechanism is an electric cylinder, there are two electric cylinders, the two electric cylinders are symmetrically arranged on the two sides of the connecting rod, the fixed end of the electric cylinder is fixedly connected with the lower surface of the top of the door-shaped frame, the telescopic end is fixedly connected with the top of the outer wall of the liquid storage bottle, the connecting plate is detachably fixedly connected with the connecting rod, and the telescopic end of the electric cylinder is detachably fixedly connected with the outer wall of the liquid storage bottle.

8. The automatic biochip sample adding and detecting device according to claim 7, characterized in that: The upper part of the detection hole is formed with a splash-proof groove in the shape of a flared mouth.

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